A pressure-controllable rat coccygeal intervertebral disc annular compression device

Abstract

A pressure-controllable rat coccygeal intervertebral disc annular pressurizing device belongs to the field of testing. It includes a fixing component, a controllable pressure component, a pressure detection component and an on-site display component; the fixing component includes an annular first and second fixing plates; a pair of side ear type fixing seats and / or a pair of ring ear type fixing seats are arranged on each fixing plate; the controllable pressure component includes a fixing screw; one end of the fixing screw is fixedly connected to the first fixing plate, and the other end passes through the second fixing plate, and a spring and an adjusting nut are set in the package; the pressure detection component includes a pressure sensor; the on-site display component includes a pressure sensor display module with a display screen; the signal output end of the pressure sensor is correspondingly connected to the signal input end of the pressure sensor display module. It adopts a thin-film fixing component structure and a lightweight controllable pressure component, and uses mice as experimental subjects. The pressure process is continuous, stable and visualized on-site, which can meet the needs of multiple groups of simultaneous and experimental data comparison.

Description

Technical Field

[0001] The invention belongs to the field of testing devices, and in particular relates to an annular pressurizing device for continuously applying pressure to a rat coccygeal intervertebral disc and performing measurement. Background Art

[0002] The intervertebral disc is an important component of the spine. Its main function is to absorb, cushion and transfer the load on the human body. At the same time, its existence allows the spine to move within a certain range.

[0003] In order to further clarify the relationship between load and intervertebral disc degeneration, as early as the 1990s, researchers installed loading devices on the caudal vertebrae of experimental animals such as mice to conduct loading experiments on intervertebral discs.

[0004] At that time, rats (also known as large white rats; Rat; Rattusnorvegicus) were usually selected as research subjects for this type of experimental animals. Male rats can weigh up to 300-400g, female rats can weigh up to 180-270g, and adult rats are generally not less than 18-20cm in length, with a coccygeal disc diameter of about 2.5-3.5mm, which is convenient for operation when establishing animal models, so they are ideal experimental animals.

[0005] In this regard, in the utility model patent with the authorization announcement date of August 10, 2018 and the authorization announcement number CN 207708039 U, a clear explanation is given: "Animal experiments play a very important role in understanding, improving and testing the research on biological interventions for human intervertebral disc degeneration. It is also the main way to study various treatment methods for blocking or reversing intervertebral disc degeneration. At present, rats are mostly used as experimental animals in the study of intervertebral disc degeneration. The anatomical position of the rat's coccygeal disc is relatively ideal, and degeneration can be induced through easy experimental operations, making it an ideal model for intervertebral disc degeneration / regeneration research" (see paragraph

[0002] of its specification).

[0006] However, with the development of science and technology, in order to reduce experimental costs and increase the number of sample comparisons, attempts have been made in recent years to use mice (Mouse; Musculus) as experimental animals.

[0007] Because mice are small in size (adult males weigh 20-40g, females weigh 18-35g), they are easy to raise and manage and easy to control; they reproduce quickly, and we have a large number of inbred strains, mutant strains and closed groups with different characteristics, and have formed a variety of internationally recognized standard strains. They are gradually being widely used in various experimental research.

[0008] More importantly, for the study of gene-related intervertebral disc diseases, the current genetically engineered mice are mostly based on mice as the background to carry out knockdown or adjustment of target genes. Therefore, establishing a mouse intervertebral disc experimental model is an indispensable and important tool for studying gene-related intervertebral disc diseases.

[0009] The Chinese utility model patent with the authorization announcement date of December 23, 2015 and the authorization announcement number of CN 204890247 U discloses a rat coccygeal pressure device, which is composed of a fixing device and a support and pressure external frame. The fixing device has two head and tail parts with the same structure, both of which are composed of left and right parts. The upper and lower horizontal areas of the main body layer of the left part each have a hollow groove, and the tooth groove inside it is buckled with the tooth groove on the right part of the tongue piece, so that the rubber layer on the inner surface of the main body layer is in close contact with the rat's tail. By pressing down the buckle lock on the tongue piece, it enters the buckle hole, further increasing the buckle between the tooth grooves, so that the entire fixing device is in closer contact with the rat's tail. The support and pressure external frame of this technical solution can be inserted into the support and pressure groove on the side of the fixing device, and by adjusting the distance between the stud groove rod and the socket-shaped connecting rod on the head side foot frame, pressure can be applied to a single side or the entire intervertebral disc and cartilage end plate. However, this technical solution is a unilateral force application and cannot achieve balanced annular pressurization. During the pressurization process, there are lateral or rotational axial stresses that cannot be eliminated and measured, and it is impossible to achieve measurable and precise pressurization, and it cannot be used for comparison between groups with different treatments and different treatments.

[0010] The Chinese utility model patent with the authorization announcement date of August 10, 2018 and the authorization announcement number of CN 207708039 U discloses a dynamic pressurization device for the rat coccygeal intervertebral disc, including a guide rod, a pressurization motor, and three concentrically arranged fixing rings mounted on the guide rod; the pressurization motor is used to drive the first fixing ring and the third fixing ring to move relative to each other in the left and right directions; the third fixing ring is provided with a pressure sensor, and the sensing head of the pressure sensor is against the second fixing ring; the first fixing ring and the second fixing ring are each equipped with two Kirschner wires, and the two fixing rings are provided with radial through holes for the two Kirschner wires to radially penetrate the fixing rings in a cross manner. This technical solution can achieve accurate and timed pressurization of the rat coccygeal intervertebral disc and induce disc degeneration. However, due to the complex structure of the entire device, the high implementation cost, and the use of a motor drive, there are many risks that affect the experimental results, such as a high failure rate and pressurization failure. In addition, due to the large overall weight of the device, it is only suitable for rats as experimental subjects. When it is used on mice, due to the large size and weight of the test device, the mouse's tail cannot move, which will cause gravity-induced intervertebral disc degeneration. Therefore, this technical solution cannot be implemented and applied on mice.

[0011] The Chinese invention patent application with the application publication date of February 22, 2019 and the application publication number of CN 109363790 A discloses a coccyx intervertebral disc degeneration experimental device, including a pull rod seat, and a coccyx sleeve on the left and the right, a guide needle cylinder is fixed to the lower part of the left coccyx sleeve, and a guide needle rod is fixed to the lower part of the right coccyx sleeve; a pin insertion hole is provided at the lower part of the cylinder wall of the left coccyx sleeve, and the pin insertion hole is connected to the upper end of the cylinder mouth of the guide needle cylinder; a pin insertion guide hole is provided on the cylinder wall of the right coccyx sleeve, and the pin insertion guide hole is a line segment-shaped through hole extending from bottom to top along the circumference of the right coccyx sleeve; a straight groove is provided on the outer surface of the guide needle rod, which runs through from top to bottom, and the upper end of the straight groove is connected to the lower end of the pin insertion guide hole; a pull rod on the left and the right is hinged on the pull rod seat, the left end of the left pull rod is hinged to the guide needle cylinder, and the right end of the right pull rod is hinged to the guide needle rod. This technical solution involves bending and pressurizing the mouse intervertebral disc, rather than axial pressurization in four directions. Therefore, uniform pressure cannot be achieved for the disc-shaped intervertebral disc. Furthermore, since the elastic modulus of the tail of each mouse is different, the repeatability of bending and pressurization is poor, which is not conducive to data comparison between groups of test subjects (or between different mice).

[0012] The Chinese invention patent application with application publication date of July 12, 2019 and application publication number of CN 109998727 A discloses an experimental device for rat coccygeal disc degeneration. It includes four fixing bolts, a fixing unit and a clamping unit. The fixing unit is used to fix the coccyx of the experimental rat, and the clamping unit is used to apply pressure to part of the coccyx of the fixed rat. It can be used to fix the coccyx of rats of different lengths. It has a wide range of applications and is convenient for in-depth exploration of the influencing factors, pathogenesis and effectiveness of various treatment methods of intervertebral disc degeneration. Similarly, due to the large overall weight of the device, it is only suitable for rats as experimental subjects. It cannot be applied to mice, which limits the use of knockout mice as experimental subjects.

[0013] With the continuous development and improvement of experimental methods and data statistical models, the model of modern animal testing projects is moving towards low cost and refinement, with increasingly higher requirements for the consistency and reproducibility of test data, and the need for differentiated comparison of test data. In this situation, large statistical samples, multiple groups and refinement of test conditions are becoming the development trend of animal testing. At the same time, as the important role of genomic research in the treatment of human diseases continues to emerge, the establishment of a mouse intervertebral disc model suitable for cultivation and production by genetic modification technology, and the guarantee of the consistency, comparability, measurability and economy of the model, have important scientific research and clinical practice significance for the cognition and treatment of intervertebral disc-related diseases.

[0014] Since the aforementioned various existing technical solutions have not taken into account the physiological structure characteristics of mice, when mice are used as animal test subjects, the aforementioned various test devices cannot meet the actual needs when mice are used as test subjects. It is difficult to obtain various test data that conform to the survival of mice in natural conditions, and it is unable to meet the test requirements under modern test models.

[0015] How to meet the new requirements of modern animal experiments, such as large statistical samples, multiple groups simultaneously, and refined experimental data comparison, when using mice as experimental subjects is an urgent problem to be solved in actual experimental work. Summary of the invention

[0016] The technical problem to be solved by the present invention is to provide a pressure-controllable rat tail intervertebral disc annular pressure device. The device uses mice as experimental pressure objects, adopts a thin-film fixed component structure and a lightweight controllable pressure component, and performs stable and continuous annular pressure loading on the intervertebral disc of the mouse tail in an experimental environment that does not affect the normal activities of the mouse. At the same time, the experimental data is visualized on-site, so that the pressure process is continuous, stable and visualized, which is conducive to ensuring the consistency of experimental data, facilitating the simultaneous comparison of multiple groups and the differentiated comparison of experimental data, and can meet the actual needs of modern animal experiments such as multi-sample data statistics, multiple groups at the same time, and refined experimental data comparison.

[0017] The technical solution of the present invention is to provide a pressure-controllable rat coccygeal intervertebral disc annular pressurizing device, which is characterized by:

[0018] The rat coccygeal intervertebral disc annular pressurizing device is composed of a fixing component, a controllable pressure component, a pressure detection component and an on-site display component;

[0019] The fixing assembly includes a first annular fixing plate and a second fixing plate; the first fixing plate and the second fixing plate are arranged parallel to each other; two puncture needles are arranged on each fixing plate, and the two puncture needles include a first puncture needle and a second puncture needle arranged crosswise; the first puncture needle is arranged parallel to the plane where the fixing plate is located; the second puncture needle is arranged to coincide with the plane where the fixing plate is located.

[0020] The controllable pressure component includes at least two sets of fixed screws; one end of the fixed screw is fixedly connected to the first fixed plate; the other end of the fixed screw passes through the second fixed plate and is fitted with a spring, and an adjusting nut is provided on the fixed screw at the end side of the spring.

[0021] The pressure detection assembly includes a gasket arranged outside the second fixing plate and a pressure sensor arranged between the second fixing plate and the gasket; the gasket is set on at least one fixing screw; the gasket is arranged between the outside of the second fixing plate and the spring;

[0022] The local display component comprises a pressure sensor display module with a display screen; the signal output end of the pressure sensor is correspondingly connected to the signal input end of the pressure sensor display module through a signal transmission line.

[0023] By changing the positions of the corresponding adjusting nuts on the fixing screws, the at least two sets of fixing screws can adjustably change the magnitude of the pressure applied to the first and second fixing plates;

[0024] The pressure value detected by the pressure sensor is displayed on-site through the pressure sensor display module.

[0025] Specifically, the first fixing plate and the second fixing plate are respectively arranged to correspond to the coccygeal vertebrae of a mouse tail; the first fixing plate or the second fixing plate is arranged to intersect with the longitudinal axis of the corresponding coccygeal vertebrae.

[0026] Furthermore, the first fixing plate and the second fixing plate are respectively and correspondingly mounted on two adjacent coccygeal vertebrae of the mouse tail.

[0027] Furthermore, the first fixing plate and the second fixing plate are fixed to the coccygeal vertebrae of two adjacent rat tails respectively through the first puncture needle and the second puncture needle which are arranged crosswise.

[0028] A pair of side ear type fixing seats are arranged on each fixing plate; a side ear puncture needle fixing hole is arranged through each side ear type fixing seat, and the first puncture needle passes through the side ear puncture needle fixing hole and is arranged on one side of the fixing plate.

[0029] Specifically, the first puncture needle passes through the auricular puncture needle fixing hole and is arranged on one side of the fixing plate parallel to the plane where the fixing plate is located.

[0030] A pair of ring-ear type fixing seats are arranged on the body of each fixing plate; a ring-ear type fixing seat is penetrated by a ring-ear puncture needle fixing hole, and the second puncture needle penetrates the ring-ear puncture needle fixing hole and is arranged on the body of the fixing plate to overlap with the plane where the fixing plate is located.

[0031] Specifically, the thickness of the fixing plate body is smaller than the diameter of the puncture needle.

[0032] The rat coccygeal intervertebral disc annular pressurizing device adopts a side-ear or ring-ear puncture needle fixing seat structure, so that the thickness of the fixing plate body can be smaller than the diameter of the puncture needle, thereby realizing the thinning of the fixing plate.

[0033] Specifically, the pressure sensor is a flexible film pressure sensor, a resistive film pressure sensor or a piezoresistor.

[0034] The controllable pressure-applying assembly comprises four fixing screws, a spring sleeved on each fixing screw, and an adjusting nut arranged on each fixing screw; the four fixing screws are evenly arranged along the circumferential direction of the first and second fixing plates; by adjusting the adjusting nut on each fixing screw, continuous and stable annular pressure loading on the first and second fixing plates can be achieved;

[0035] The rat coccygeal intervertebral disc annular pressurizing device adopts additive manufacturing technology or 3D printing technology to manufacture the first and second fixing plate bodies of high-strength resin. On the one hand, it can reduce the weight of the overall device and avoid gravity-induced intervertebral disc degeneration; on the other hand, it can improve the manufacturing accuracy of the device and can be produced and used inexpensively and in large quantities.

[0036] Compared with the prior art, the advantages of the present invention are:

[0037] 1. This technical solution adopts a side-ear and ring-ear puncture needle fixing seat structure, so that the thickness of the fixing plate body can be smaller than the diameter of the puncture needle, realizing the thinning and lightness of the fixing plate, reducing the weight and volume of the largest component in the entire pressure-applying device, and will not hinder the normal movement of the mouse tail, so that the entire experimental device can be applied to mice weighing only tens of grams, and will not cause gravity-induced intervertebral disc degeneration, which is conducive to obtaining more realistic experimental data; at the same time, the application of the thin-film device can establish single-segment intervertebral disc pressurization of mice, solving the dilemma that only multi-segment mouse intervertebral disc pressurization can be used in previous literature;

[0038] 2. With this technical solution, the applied pressure is constant and adjustable, the applied pressure can be measured in real time, the operation is convenient, and the fixed ring bears pressure evenly, which can achieve continuous pressure on the coccyx of mice without bias pressure. The vertical double-needle puncture can avoid shear stress and axial stress during the pressurization process, and can simulate the actual pressure of the normal intervertebral disc;

[0039] 3. This technical solution can achieve constant and controllable pressure on the intervertebral disc between the two coccygeal vertebrae of the mouse's tail, thereby inducing pressure-induced intervertebral disc degeneration and establishing an animal intervertebral disc degeneration model, thereby providing favorable conditions for the study of intervertebral disc degeneration;

[0040] 4. This technical solution adopts a thin-film fixed component structure and a lightweight controllable pressure component to apply stable and continuous annular pressure to the intervertebral disc of the mouse's tail in an experimental environment that does not affect the normal activities of the mouse. At the same time, the experimental data is visualized on-site, which is particularly suitable for spinal experimental projects using mice as experimental subjects;

[0041] 5. Since the entire pressure-applying device of this technical solution is light in weight and has little impact on the test object, data collection of gradient injury experiments can be realized;

[0042] 6. As the whole experimental device is precisely positioned and has low preparation cost, it can display pressure data in real time, which is particularly suitable for simultaneous multiple and multiple group tests with large samples, and helps to achieve consistency of test data and compare the differences of test data;

[0043] 7. This technical solution uses an adjustable and visualized solution, which can establish intervertebral disc degeneration models with different compression degrees and can be used for research on different degrees of intervertebral disc degeneration.

[0044] 8. Since the anatomical structure of mice is smaller than that of rats, the traditional metal processing technology cannot achieve the precision and batch requirements required by the pressurized device, and the processing cost is relatively high; this technical solution uses additive manufacturing technology (3D printing) to make a high-strength resin fixing plate body, which on the one hand greatly reduces the weight of the overall device and avoids gravity-induced intervertebral disc degeneration; on the other hand, it can improve the accuracy of the device and can be produced and used inexpensively and in large quantities. BRIEF DESCRIPTION OF THE DRAWINGS

[0045] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0046] Figure 2 It is a structural schematic diagram of an embodiment of a fixing plate of the present invention;

[0047] Figure 3 It is a schematic diagram of the structure of the gasket of the present invention;

[0048] Figure 4 is a schematic structural diagram of another embodiment of a fixing plate of the present invention;

[0049] Figure 5 This is a schematic diagram of the structure of the fixing plate after the puncture needle is inserted into the present invention;

[0050] Figure 6 It is a reference diagram of the use status of the entire device of the present invention.

[0051] In the figure, 1-1 is the first fixing plate, 1-2 is the second fixing plate, 1-3 is the fixing screw through hole, 1-4 is the side ear type fixing seat, 1-5 is the side ear puncture needle fixing hole, 1-6 is the second puncture needle fixing hole, 1-6a is the ring ear puncture needle fixing hole, 1-7 is the ring ear type fixing seat, 2-1 is the first puncture needle, 2-2 is the second puncture needle, 3 is the fixing screw, 4 is the spring, 5 is the adjusting nut, 6 is the flexible film pressure sensor, 7 is the signal transmission line, 8 is the detection display module, 9-1 is the first coccygeal vertebra, 9-2 is the second coccygeal vertebra, 10 is the intervertebral disc, 11 is the gasket, and 11-1 is the gasket fixing screw hole. DETAILED DESCRIPTION

[0052] The present invention will be further described below in conjunction with the accompanying drawings.

[0053] Figure 1 The technical solution of the present invention provides a pressure-controllable rat coccygeal intervertebral disc annular pressurizing device, the invention point of which is:

[0054] The rat coccygeal intervertebral disc annular pressurizing device is composed of a fixing component, a controllable pressure-applying component, a pressure detection component and an on-site display component.

[0055] The fixing assembly includes a first annular fixing plate 1-1 and a second fixing plate 1-2; the first fixing plate and the second fixing plate are arranged parallel to each other; and two puncture needles (also called Kirschner wires) are arranged on each fixing plate.

[0056] The two puncture needles are arranged crosswise.

[0057] Depend on Figure 5 It can be seen from the above that the two puncture needles are arranged parallel to or coincident with the plane where the radial axis of the corresponding fixing plate is located (referred to as the plane where the fixing plate is located, the same below).

[0058] Among them, the first puncture needle 2-1 of the two puncture needles is arranged parallel to the plane where the fixing plate is located, and the second puncture needle 2-2 of the two puncture needles is arranged to coincide with the plane where the fixing plate is located.

[0059] like Figure 1 As shown in the figure, the controllable pressure assembly includes at least two sets of fixed screws 3; one end of the fixed screw is fixedly connected to the first fixed plate ( Figure 1 The structure shown in the figure is that the fixing screw is fixed by means of two nuts locking relative to each other), the other end of the fixing screw passes through the second fixing plate, and a spring 4 is set in the suit, and an adjusting nut 5 is set on the fixing screw at the end side of the spring.

[0060] Since one end of the fixing screw is fixedly connected to the first fixing plate, the pressure of the spring on the second fixing plate can be adjusted by adjusting the adjusting nut on the fixing screw at the end side of the spring, that is, the inward pressure between the first and second fixing plates is adjusted.

[0061] Since the first and second positioning pieces are respectively fixed to the coccygeal vertebrae of the experimental mice, the first and second positioning pieces can exert pressure towards each other to achieve the effect of positioning the two adjacent coccygeal vertebrae ( Figure 1 The pressure exerted by the first coccygeal vertebra 9-1 and the second coccygeal vertebra 9-2 eventually causes the intervertebral disc 10 between the two adjacent coccygeal vertebrae to be squeezed, thereby achieving uniform pressure on the disc-shaped intervertebral disc.

[0062] Depend on Figure 1 and Figure 5As shown, in the present technical solution, based on the consideration of uniform force distribution, the controllable pressure components composed of a fixed screw, a spring sleeved on the fixed screw, and an adjusting nut arranged on the outer side of the spring end are usually arranged in two or four groups. Each controllable pressure component is evenly distributed on the annular sheet of the first fixed sheet and the second fixed sheet along the circumference of the same concentric circle with the axial center of the fixed sheet as the center. By changing the position of the corresponding adjusting nut on the fixed screw, the at least two groups of fixed screws can adjustably change the magnitude of the pressure applied to the first and second fixed sheets.

[0063] like Figure 1 As shown, in actual implementation, four groups of controllable pressure components are used; four fixing screws are evenly distributed along the circumferential direction of the first and second fixing plates; by adjusting the adjusting nut on each fixing screw, continuous and stable annular pressure loading on the first and second fixing plates can be achieved.

[0064] The pressure detection assembly includes a gasket arranged outside the second fixing plate and a pressure sensor 6 arranged between the second fixing plate and the gasket.

[0065] The gasket set is arranged on at least one fixing screw rod, such as Figure 3 As shown in , at least one gasket fixing screw hole 11 - 1 is opened on the gasket 11 , the gasket is arranged between the outer side of the second fixing plate and the spring, and the pressure sensor is arranged between the outer side of the second fixing plate and the gasket.

[0066] In this technical solution, the purpose of providing the gasket is to enable the pressure sensor to evenly sense the pressure between the outer side of the second fixing plate and the spring. In other words, it is to balance the pressure applied by the end of the spring to the second fixing plate.

[0067] like Figure 1 As shown in , the local display component includes a pressure sensor display module 8 with a display screen; the signal output end of the pressure sensor 6 is correspondingly connected to the signal input end of the pressure sensor display module through a signal transmission line 7.

[0068] The pressure value detected by the pressure sensor is displayed locally through the pressure sensor display module.

[0069] like Figure 1 As shown in the figure, the first fixing plate and the second fixing plate respectively correspond to the coccygeal vertebrae of a mouse tail and are arranged in a set; the first fixing plate or the second fixing plate is arranged to intersect with the longitudinal axis of the corresponding coccygeal vertebrae.

[0070] Specifically, the first fixing plate and the second fixing plate are respectively and correspondingly mounted on two adjacent coccygeal vertebrae of the mouse tail.

[0071] Furthermore, the first fixing plate and the second fixing plate are fixed to the coccygeal vertebrae of two adjacent rat tails respectively through the first puncture needle and the second puncture needle which are arranged crosswise.

[0072] like Figure 2 and Figure 5 As shown in the figure, on each fixing sheet (the first fixing sheet 1-1 is taken as an example in the figure), fixing screw through holes 1-3 corresponding to the number of the fixing screws are provided.

[0073] At the same time, on each fixing plate (to be precise, on one side of each fixing plate), a pair (i.e. two) side ear type fixing seats 1-4 are arranged, and on each side ear type fixing seat, a side ear puncture needle fixing hole (also called the first puncture needle fixing hole) 1-5 is arranged through it, and the first puncture needle passes through the side ear puncture needle fixing hole and is arranged on one side of the fixing plate.

[0074] Further, the first puncture needle passes through the auricular puncture needle fixing hole 1-5 and is arranged on one side of the fixing plate in parallel with the plane where the fixing plate is located (see Figure 5 as shown).

[0075] exist Figure 2 In the embodiment, a second puncture needle fixing hole 1-6 is provided on the fixed body, and the second puncture needle passes through the second puncture needle fixing hole and is arranged on the body of the fixing plate so as to coincide with the plane where the radial axis of the fixing plate is located.

[0076] Or, if Figure 4 As shown in the figure, a pair (i.e. two) of ring-ear type fixing seats 1-7 are arranged on the body of each fixing plate, and a ring-ear puncture needle fixing hole 1-6a is arranged through each ring-ear type fixing seat, and the second puncture needle 2-2 passes through the ring-ear puncture needle fixing hole and is arranged on the body of the fixing plate so as to coincide with the plane where the fixing plate is located.

[0077] Depend on Figure 2 , Figure 4 or Figure 5 As shown in the figure, the side ear type fixing seat 1-4, the second puncture needle fixing hole 1-6 or the ring ear type fixing seat 1-7 in the present technical scheme are all arranged along the radial direction of the fixing plate. Naturally, the side ear puncture needle fixing hole 1-5 or the ring ear puncture needle fixing hole 1-6a is also a radial through hole.

[0078] Use Figure 4 In the case of the fixing plate of the structure shown, the thickness of the fixing plate body may be smaller than the diameter of the puncture needle.

[0079] The technical solution of the present invention adopts a side-ear or ring-ear type puncture needle fixing seat structure and a fixing method for the puncture needle, so that the thickness of the fixing plate body can be smaller than the diameter of the puncture needle, thereby realizing the thinning of the fixing plate, and thus fundamentally reducing the weight and volume of the largest component in the experimental device (referring to the fixing ring or the fixing plate), so that the entire experimental device can be suitable for mice weighing only tens of grams, and will not hinder the normal movement of the mouse's tail, will not cause gravity-induced intervertebral disc degeneration, and is conducive to obtaining more realistic experimental data.

[0080] Figure 6 In the figure, a reference diagram of the usage status of the device described in the present technical solution is given. From the ratio of the overall volume of the test subject mouse and the present device shown in the figure, it can be seen that the implementation of the present technical solution can minimize the impact of the pressurizing device on the normal activity of the mouse's tail.

[0081] The technical solution of the present invention, due to the use of an adjustable and visualized solution, can establish intervertebral disc degeneration models with different compression degrees, and can be used for research on different degrees of intervertebral disc degeneration.

[0082] Since the anatomical structure of mice is smaller than that of rats, the traditional metal processing technology cannot achieve the precision and batch production requirements required by the pressurizing device in this technical solution, and the processing cost is relatively high.

[0083] The technical solution of the present invention adopts additive manufacturing technology (3D printing) to manufacture a high-strength resin pressurizing device (which mainly involves the manufacturing method and manufacturing process of a fixing ring or a fixing plate). On the one hand, it greatly reduces the weight of the overall device and can avoid gravity-induced intervertebral disc degeneration; on the other hand, it can improve the accuracy of the device and can be produced and used inexpensively and in large quantities.

[0084] In the technical solution of the present invention, the pressure sensor is a flexible film pressure sensor, and various commercially available resistive film pressure sensors or varistors can be selected as long as their measuring range meets the permitted range of the product test pressure.

[0085] The pressure sensor display module can select the commercially available flexible film pressure sensor detection module MY2801, which can display the piezoresistance AD ​​value digital display curve, has a small size, light weight, long service life, and low price, and can fully meet the needs of conducting multiple groups of experiments simultaneously.

[0086] The technical solution of the present invention adopts a thin-film fixing component structure and a lightweight controllable pressure component to perform stable and continuous annular pressure loading on the intervertebral disc of the mouse's tail in an experimental environment that minimizes the impact on the normal activities of the mouse. At the same time, the experimental data is visualized on-site, so that the pressure process is continuous, stable and visualized, which is conducive to ensuring the consistency of experimental data. It is particularly suitable for the simultaneous implementation of multiple and multiple groups of experiments with large samples, which helps to achieve consistency of experimental data and compare the differences of experimental data. It can meet the actual needs of modern animal experiments such as multi-sample data statistics, simultaneous implementation of multiple groups and refined experimental data comparison.

[0087] The invention can be widely used in various rat tail intervertebral disc test fields using mice as test objects.

Claims

1. A pressure-controllable rat coccygeal intervertebral disc annular pressurizing device, comprising a fixing assembly and a controllable pressure-applying assembly, wherein the fixing assembly comprises an annular first fixing plate and a second fixing plate, the first fixing plate and the second fixing plate are arranged parallel to each other, and two puncture needles are arranged on each fixing plate, and the two puncture needles include a first puncture needle and a second puncture needle arranged crosswise; wherein: The rat coccygeal intervertebral disc annular pressurizing device also includes a pressure detection component and an on-site display component; The thickness of the fixing plate bodies of the first fixing plate and the second fixing plate is smaller than the diameter of the puncture needle; A pair of side ear type fixing seats are arranged on each fixing plate; a side ear puncture needle fixing hole is arranged through each side ear type fixing seat, and the first puncture needle passes through the side ear puncture needle fixing hole and is arranged on one side of the fixing plate; A pair of ring-ear type fixing seats are arranged on the body of each fixing plate; a ring-ear type fixing seat is penetrated by a ring-ear puncture needle fixing hole, and the second puncture needle penetrates the ring-ear puncture needle fixing hole and is arranged on the body of the fixing plate so as to overlap with the plane where the fixing plate is located; The first puncture needle is arranged parallel to the plane where the fixing plate is located; the second puncture needle is arranged to coincide with the plane where the fixing plate is located; The rat coccygeal intervertebral disc annular pressurizing device adopts a side-ear or ring-ear puncture needle fixing seat structure and a puncture needle fixing method, so that the thickness of the fixing plate body can be smaller than the diameter of the puncture needle, thereby realizing the thinning of the fixing plate; The controllable pressure assembly includes at least two sets of fixed screws; one end of the fixed screw is fixedly connected to the first fixed plate; the other end of the fixed screw passes through the second fixed plate and is fitted with a spring, and an adjusting nut is provided on the fixed screw at the end side of the spring; The pressure detection assembly includes a gasket arranged outside the second fixing plate and a pressure sensor arranged between the second fixing plate and the gasket; The gasket set is arranged on at least one screw rod; The gasket is arranged between the outer side of the second fixing plate and the spring; The local display assembly includes a pressure sensor display module with a display screen; the signal output end of the pressure sensor is connected to the signal input end of the pressure sensor display module via a signal transmission line; By changing the position of the corresponding adjusting nut on the screw rod, the at least two sets of fixing screw rods can adjustably change the pressure applied to the first and second fixing plates; The pressure value detected by the pressure sensor is displayed on-site through the pressure sensor display module; The rat tail intervertebral disc annular pressurizing device makes the entire experimental device suitable for mice weighing only tens of grams, and will not hinder the normal movement of the mouse's tail, will not cause gravity-induced intervertebral disc degeneration, and is conducive to obtaining more realistic experimental data; The pressure-controllable rat tail intervertebral disc annular pressurizing device adopts a thin-film fixed component structure and a lightweight controllable pressure component. In an experimental environment that does not affect the normal activities of the mouse, it can load the intervertebral disc of the mouse tail with stable and continuous annular pressure, and at the same time visualize the experimental data on the spot, so that the pressure application process is continuous, stable and visualized, which is conducive to ensuring the consistency of experimental data, facilitating the simultaneous comparison of multiple groups and the differentiated comparison of experimental data, and can meet the actual needs of multi-sample data statistics, multiple groups at the same time and refined test data comparison.

2. The pressure-controllable rat coccygeal intervertebral disc annular pressurizing device according to claim 1, characterized in that The first fixing plate and the second fixing plate are respectively set corresponding to the coccygeal vertebrae of a mouse tail; The first fixing plate or the second fixing plate is arranged to intersect with the longitudinal axis of the corresponding coccygeal vertebra.

3. The pressure-controllable rat coccygeal intervertebral disc annular pressurizing device according to claim 2, characterized in that The first fixing plate and the second fixing plate are respectively and correspondingly mounted on two adjacent coccygeal vertebrae of the mouse tail.

4. The pressure-controllable rat coccygeal intervertebral disc annular pressurizing device according to claim 3, characterized in that The first fixing plate and the second fixing plate are fixed to the coccygeal vertebrae of two adjacent rat tails respectively through the first puncture needle and the second puncture needle which are arranged crosswise.

5. The pressure-controllable rat coccygeal intervertebral disc annular pressurizing device according to claim 1, characterized in that The first puncture needle passes through the auricular puncture needle fixing hole and is arranged on one side of the fixing plate parallel to the plane where the fixing plate is located.

6. The pressure-controllable rat coccygeal intervertebral disc annular pressurizing device according to claim 1, characterized in that The pressure sensor is a flexible film pressure sensor, a resistive film pressure sensor or a piezoresistor; The controllable pressure assembly includes four fixed screws, a spring sleeved on each fixed screw, and an adjusting nut arranged on each fixed screw; The four fixing screws are evenly arranged along the circumferential direction of the first and second fixing plates; by adjusting the adjusting nut on each fixing screw, continuous and stable annular pressure loading on the first and second fixing plates can be achieved; The rat coccygeal intervertebral disc annular pressurizing device adopts additive manufacturing technology or 3D printing technology to manufacture the first and second fixing plate bodies of high-strength resin. On the one hand, it can reduce the weight of the overall device and avoid gravity-induced intervertebral disc degeneration; on the other hand, it can improve the manufacturing accuracy of the device and can be produced and used inexpensively and in large quantities.

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