Antibacterial Chinese medicine composition and its application

Abstract

The present invention relates to the technical field of traditional Chinese medicine, and in particular to an antibacterial Chinese medicine composition and its application. The Chinese medicine composition of the present invention includes 1-10 parts of sappan wood extract, 1-3 parts of sanguisorba officinalis charcoal extract, 1-10 parts of rose extract, 1-5 parts of blood-seeing shrew extract, 1-5 parts of madder charcoal extract, 1-5 parts of hawthorn extract, and 1-5 parts of stir-fried tribulus extract. The Chinese medicine composition has a very significant inhibitory effect on Acinetobacter baumannii, and can play a synergistic effect with antibiotics such as imipenem and minocycline, significantly improving the therapeutic effect of Acinetobacter baumannii infection.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicines, and in particular to an antibacterial traditional Chinese medicine composition and application thereof. Background Art

[0002] Acinetobacter baumannii is a strictly aerobic, non-lactose fermenting Gram-negative bacterium that does not have flagella and has strong survival ability. It is a conditional pathogen. Acinetobacter baumannii is a common Acinetobacter bacteria in hospital infections and has become one of the important pathogens of hospital infections such as intensive care units. It mainly causes respiratory tract infections and can also cause sepsis, urinary tract infections, secondary meningitis, etc.

[0003] The treatment of Acinetobacter baumannii infection mainly uses antibiotics, but Acinetobacter baumannii is very easy to develop resistance to antibiotics. The emergence of multidrug-resistant Acinetobacter baumannii or extensively drug-resistant Acinetobacter baumannii in clinical practice has caused great difficulties in the control and treatment of Acinetobacter baumannii infection. Compared with antibiotics, traditional Chinese medicine has the advantages of more targets and lower risk of drug resistance. Therefore, it is of great significance to develop a traditional Chinese medicine composition that has a significant inhibitory effect on Acinetobacter baumannii. Summary of the invention

[0004] The purpose of the present invention is to provide an antibacterial Chinese medicine composition, which has a very significant inhibitory effect on Acinetobacter baumannii. Another purpose of the present invention is to provide an application of the antibacterial Chinese medicine composition.

[0005] To achieve the above purpose, the present invention is based on the study of the physiological metabolic characteristics of Acinetobacter baumannii and its interaction mechanism with the human body, and develops a Chinese medicine composition with a strong inhibitory effect for Acinetobacter baumannii. The present invention found in the research and development process that although many Chinese medicine components have different degrees of inhibitory effects on Acinetobacter baumannii, the inhibitory effects of these Chinese medicine components on Acinetobacter baumannii still need to be improved; the present invention attempts to compound different Chinese medicine components, and finds that many Chinese medicine components with inhibitory effects on Acinetobacter baumannii cannot significantly improve their antibacterial effects after compounding, and even some components compounding can reduce the antibacterial effect. The present invention unexpectedly found that the rose extract, which has a weak inhibitory effect on Acinetobacter baumannii, is compounded with the sappanwood extract, which has an inhibitory effect on Acinetobacter baumannii, which can significantly improve the inhibitory effect of sappanwood on Acinetobacter baumannii, and can also improve the body's immunity, and the antibacterial effect and immunomodulatory effect promote each other, and the treatment effect of infection by the conditional pathogen Acinetobacter baumannii is significant.

[0006] Based on the above findings, the present invention provides an antibacterial Chinese medicine composition, which comprises a sappan wood extract and a rose extract in a mass ratio of (1-10):2.

[0007] The present invention further found that, compared with the extracts of other rose tissues, the rose flower extract can cooperate better with the sappan wood extract to produce a synergistic effect on the inhibition of Acinetobacter baumannii.

[0008] Preferably, the rose extract is a rose flower extract;

[0009] If rose extract is used, the mass ratio of the sappan wood extract to the rose extract in the traditional Chinese medicine composition is (1-5):2.

[0010] On the basis of the above-mentioned composite composition of sappan wood extract and rose flower extract, adding Sanguisorba officinalis charcoal extract can further improve the inhibitory effect of the composition on Acinetobacter baumannii.

[0011] The traditional Chinese medicine composition of the present invention also includes Sanguisorba officinalis charcoal extract. In the traditional Chinese medicine composition, the mass ratio of the Sanguisorba officinalis charcoal extract to the Sappan wood extract is 1:(1-10).

[0012] Preferably, the mass ratio of the Sanguisorba officinalis charcoal extract to the Sappan wood extract is 1:(1-5).

[0013] Specifically, the present invention provides an antibacterial Chinese medicine composition, which comprises the following components in parts by weight: 1-10 parts of Sappan wood extract, 1-3 parts of rose flower extract, and 1-5 parts of Sanguisorba officinalis charcoal extract.

[0014] The sappan wood extract, rose extract and sanguisorba officinalis charcoal extract described in the invention are all water extracts.

[0015] The water extracts of Sappanwood, Rose and Sanguisorba officinalis charcoal can be purchased from the market or extracted by conventional methods in the art.

[0016] The water extracts of Sappan wood, Rose and Sanguisorba officinalis charcoal are preferably prepared by the following method: Sappan wood, Rose or Sanguisorba officinalis charcoal are decocted in water for 1-3 times, each time for 30-60 minutes, and the decoctions are combined and dried to a solid state; the solid-liquid ratio (g:ml) of each decoction is 1:(10-30).

[0017] The water extracts of sappan wood, rose and sanguisorba officinalis obtained by the above extraction method can work together better, significantly improve the inhibitory effect on Acinetobacter baumannii, and at the same time improve the body's immunity, thereby jointly promoting the treatment of Acinetobacter baumannii infection.

[0018] Preferably, the decoction is performed three times, the material-liquid ratio of the first decoction is 1:(20-25), the decoction time is 30-35 minutes, and the material-liquid ratio of the second and third decoctions is 1:(10-15), and the decoction time is 40-45 minutes.

[0019] In the above extraction methods, drying can be performed by freeze drying.

[0020] On the basis of the above composition, further adding Sanguisorba officinalis extract, Rubia cordifolia charcoal extract, Crataegus pinnatifida extract and Tribulus terrestris extract can obtain a traditional Chinese medicine composition with better inhibitory effect on Acinetobacter baumannii.

[0021] Specifically, the Chinese medicine composition comprises the following components in parts by weight: 1-10 parts of Sappan wood extract, 1-3 parts of Sanguisorba officinalis charcoal extract, 1-10 parts of rose extract, 1-5 parts of Sanguisorba officinalis charcoal extract, 1-5 parts of Crataegus pinnatifida extract, 1-5 parts of stir-fried Tribulus terrestris extract. The Chinese medicine composition of the present invention can only consist of the above components. The Chinese medicine composition can significantly inhibit Acinetobacter baumannii and improve the body's immunity at the same time, thereby improving the therapeutic effect of Acinetobacter baumannii infection from two aspects: direct antibacterial and body immunity.

[0022] As a preferred embodiment of the present invention, the Chinese medicine composition comprises the following components in parts by weight: 1-5 parts of Sappan wood extract, 1-3 parts of Sanguisorba officinalis charcoal extract, 1-5 parts of rose flower extract, 1-3 parts of Sanguisorba officinalis charcoal extract, 1-3 parts of Crataegus pinnatifida extract, 1-3 parts of Crataegus pinnatifida extract, and 1-5 parts of stir-fried Tribulus terrestris extract. The Chinese medicine composition of the present invention may consist only of the above components.

[0023] The extract of Sanguisorba officinalis, the extract of Rubia cordifolia, the extract of Crataegus pinnatifida and the extract of Tribulus terrestris described in the present invention are preferably water extracts.

[0024] The water extracts of Rhizoma Cyperi, Rhizoma Rubiae, Fructus Crataegi and Rhizoma Tribuluse can be purchased from the market or extracted by conventional methods in the art.

[0025] The water extracts of Sanguisorba officinalis, Rubia cordifolia charcoal, Hawthorn and stir-fried Tribulus terrestris are preferably prepared by the following method: Sanguisorba officinalis, Rubia cordifolia charcoal, Hawthorn or stir-fried Tribulus terrestris are decocted in water for 1-3 times, each decocting for 30-60 minutes, and the decoctions are combined and dried to a solid; the solid-liquid ratio (g:ml) of each decoction is 1:(10-30).

[0026] Preferably, the decoction is performed three times, the material-liquid ratio of the first decoction is 1:(20-25), the decoction time is 30-35 minutes, and the material-liquid ratio of the second and third decoctions is 1:(10-15), and the decoction time is 40-45 minutes.

[0027] In the above extraction methods, drying can be performed by freeze drying.

[0028] The present invention also provides application of the antibacterial Chinese medicine composition in preparing antibacterial drugs.

[0029] Regarding the above-mentioned traditional Chinese medicine composition, the present invention also found that the composition has a synergistic effect with antibiotics such as imipenem and minocycline. The combined use of the composition with antibiotics such as imipenem and minocycline can significantly improve the inhibitory effect of Acinetobacter baumannii when used alone.

[0030] The present invention provides an application of the antibacterial Chinese medicine composition in combination with an antibiotic in the preparation of an antibacterial drug, wherein the antibiotic is one or more selected from imipenem, minocycline, rifampicin, chloramphenicol and tetracycline.

[0031] The invention provides an antibacterial drug, which comprises the antibacterial traditional Chinese medicine composition and one or more selected from imipenem, minocycline, rifampicin, chloramphenicol and tetracycline.

[0032] The antibacterial drug prepared from the antibacterial Chinese medicine composition of the present invention can be in the dosage form commonly used in the pharmaceutical field, including but not limited to pills, powders, ointments, pills, tablets, granules, capsules, etc.

[0033] The antibacterial drug of the present invention consists of the traditional Chinese medicine composition and a pharmaceutically acceptable carrier.

[0034] The beneficial effects of the present invention are:

[0035] (1) The Chinese medicine composition of the present invention has a significant inhibitory effect on Acinetobacter baumannii: the in vitro antibacterial experiment verified that the diameter of the inhibition zone of the Chinese medicine composition of the present invention was significantly increased, and the minimum inhibitory concentration (MIC) was significantly reduced; the in vivo antibacterial experiment verified that the Chinese medicine composition of the present invention can inhibit the growth and reproduction of Acinetobacter baumannii in vivo, and reduce the mortality rate of animals infected with Acinetobacter baumannii.

[0036] (2) The Chinese medicine composition of the present invention can exert a synergistic effect with antibiotics such as imipenem and minocycline. Combined use with antibiotics can significantly improve the therapeutic effect of Acinetobacter baumannii infection and alleviate the development of drug resistance. BRIEF DESCRIPTION OF THE DRAWINGS

[0037] Figure 1The results of the inhibition zone experiment of the combined drug sensitivity experiment in Experimental Example 2 of the present invention, wherein A is the synergistic effect of the Chinese medicine composition of Example 1 with minocycline (MH), levofloxacin (LEV), cefoperazone sodium sulbactam (SCF), meropenem (MEM), and amikacin (AK), 1 is minocycline, 2 is amikacin, 3 is levofloxacin, 4 is cefoperazone sulbactam, and 5 is meropenem; B is the synergistic effect of the Chinese medicine composition of Example 1 with piperacillin (PRL), rifampicin (RD), cefazolin (KZ), imipenem (IPM), clindamycin (DA), ampicillin sulbactam (SAM), oxacillin (OX), ceftriaxone (CRO) , 1 is ceftriaxone, 2 is piperacillin, 3 is rifampicin, 4 is oxacillin, 5 is ampicillin sulbactam, 6 is clindamycin, 7 is imipenem, and 8 is cefazolin; C is the synergistic effect of the Chinese medicine composition of Example 1 and erythromycin (E), ampicillin (AMP), amikacin (AK), tetracycline (TE), levofloxacin (LEV), penicillin (P), cefoperazone sodium sulbactam (SCF), cefoxitin (FOX), and chloramphenicol (C), 1 is cefoperazone sulbactam, 2 is cefoxitin, 3 is erythromycin, 4 is penicillin, 5 is levofloxacin, 6 is tetracycline, 7 is amikacin, 8 is ampicillin, and 9 is chloramphenicol. DETAILED DESCRIPTION

[0038] The following examples are used to illustrate the present invention but are not intended to limit the scope of the present invention.

[0039] In the following examples, the extract of Sappan wood, the extract of Sanguisorba officinalis charcoal, the extract of Rose, the extract of Sanguisorba officinalis charcoal, the extract of Rubia cordifolia charcoal, the extract of Crataegus pinnatifida, and the extract of Tribulus terrestris were prepared by the following method:

[0040] (1) Soak the Chinese herbal medicine pieces (sappan wood, charcoal from Sanguisorba officinalis, rose, smilax glabra, charcoal from madder, hawthorn or stir-fried Tribulus terrestris) in cold water for 1 hour;

[0041] (2) Add the Chinese herbal medicine pieces soaked in step (1) and water in a stainless steel container at a solid-liquid ratio (g:ml) of 1:25, and use bamboo chopsticks to turn the medicine floating on the water surface up and down to make the Chinese herbal medicine pieces completely immersed in the water, and ensure that the water surface is 2-3 cm higher than the Chinese herbal medicine; boil the medicine over high heat and then simmer over low heat, decocting each dose three times, the first decocting time is maintained for 30 minutes after boiling, and the second and third decocting times are added with boiling water at a solid-liquid ratio (g:ml) of 1:15, and the decocting time is maintained for 40 minutes after boiling.

[0042] (3) After combining the three decoctions of the medicinal liquid in step (2), freeze-drying the mixture to a solid state, grinding the solid into powder using a grinder, putting the powder into a sealed bag, and storing the powder in a refrigerator at 4°C for later use.

[0043] Example 1

[0044] The present embodiment provides an antibacterial Chinese medicine composition, which is composed of the following components in parts by weight: 5 parts of Sappan wood extract, 1 part of Sanguisorba officinalis charcoal extract, 2 parts of rose extract, 1 part of Sanguisorba officinalis extract, 1 part of Rubia cordifolia charcoal extract, 1 part of Crataegus pinnatifida extract, and 1 part of stir-fried Tribulus terrestris extract.

[0045] Example 2

[0046] The present embodiment provides an antibacterial Chinese medicine composition, which is composed of the following components in parts by weight: 1 part of Sappan wood extract, 1 part of Sanguisorba officinalis charcoal extract, 2 parts of rose extract, 1 part of Sanguisorba officinalis extract, 5 parts of Rubia cordifolia charcoal extract, 1 part of Crataegus pinnatifida extract, and 1 part of stir-fried Tribulus terrestris extract.

[0047] Example 3

[0048] The present embodiment provides an antibacterial Chinese medicine composition, which is composed of the following components in parts by weight: 1 part of Sappan wood extract, 1 part of Sanguisorba officinalis charcoal extract, 2 parts of rose extract, 1 part of Sanguisorba officinalis extract, 1 part of Rubia cordifolia charcoal extract, 1 part of Crataegus pinnatifida extract, and 5 parts of stir-fried Tribulus terrestris extract.

[0049] Comparative Example 1

[0050] This comparative example provides an antibacterial Chinese medicinal composition, which consists only of the Sappan wood extract.

[0051] Comparative Example 2

[0052] The present comparative example provides an antibacterial Chinese medicine composition, which is composed of the following components in parts by weight: 5 parts of black plum extract, 1 part of Sanguisorba officinalis charcoal extract, 2 parts of rose extract, 1 part of Sanguisorba officinalis charcoal extract, 1 part of Rubia cordifolia charcoal extract, 1 part of hawthorn extract, and 1 part of stir-fried Tribulus terrestris extract.

[0053] Experimental Example 1 In vitro antibacterial experiment of Chinese medicine composition

[0054] 1. Detection of antibacterial activity of traditional Chinese medicine composition by inhibition zone method (diffusion method)

[0055] The antibacterial activity of the Chinese medicine composition was detected by the inhibition zone method (diffusion method), and the specific method is as follows:

[0056] (1) Activation of bacterial strains and preparation of bacterial solution: streak frozen Acinetobacter baumannii strains onto nutrient agar plates and culture at 37°C overnight; pick a single colony and inoculate into 10 ml nutrient broth liquid culture medium and culture at 37°C, 200 rpm, in a shaking incubator overnight for later use.

[0057] (2) Plate coating: dilute the bacterial solution from step (1) with sterile saline to 0.5 McFarland turbidity, take the diluted bacterial solution with a sterile swab and squeeze it on the tube wall, remove the excess bacterial solution and evenly smear it on the surface of a nutrient agar plate (product of Guangzhou Dijing Company) for three times, rotate the plate 60 degrees each time, and finally circle around the plate twice to ensure that the entire surface of the culture medium is evenly coated.

[0058] (3) Preparation of Chinese medicine composition solution: The powder of the Chinese medicine composition of each example and comparative example was dissolved in deionized water to a concentration of 200 mg / ml. The solution was sterilized by high pressure and then used.

[0059] (4) Use a sterile puncher to punch holes with a diameter of 6 mm on the solid culture medium coated with the bacterial solution; add the liquid of the Chinese medicine composition prepared in step (3) into the holes, 80 μl / well.

[0060] (5) The plate was placed in a 35°C constant temperature incubator for continued incubation overnight and the diameter of the inhibition zone was measured. The results of the inhibition zone diameter measurement are shown in Table 1.

[0061] Table 1 Diameter of inhibition zone

[0062]

[0063] 2. Microbroth dilution method to detect the antibacterial activity of Chinese medicine composition

[0064] The antibacterial activity of the Chinese medicine composition was detected by the microbroth dilution method, and the specific method is as follows:

[0065] (1) Preparation of Chinese medicine composition and culture medium: The powder of the Chinese medicine composition of each example and comparative example was dissolved in deionized water to 400 mg / ml to obtain a Chinese medicine composition solution; nutrient broth (product of Qingdao Haibo Biotechnology Co., Ltd.) was prepared according to the instructions, and sterilized by high pressure for later use.

[0066] (2) The Chinese medicine composition solution of each embodiment and comparative example was diluted in multiple ratios with sterile nutrient broth, the concentration of the first well was 400 mg / ml, and there were 11 dilutions in total; the overnight cultured Acinetobacter baumannii liquid was diluted with nutrient broth to 0.5 McLaren turbidity standard, 100 μl was added to each well, and then 100 μl of the Chinese medicine composition solution was added, and the concentrations of the Chinese medicine composition from the 1st well to the 11th well were 200, 100, 50, 25, 12.5, 6.25, 3.13, 1.57, 0.79, 0.4 and 0.2 mg / ml, respectively, and 100 μl of physiological saline was added to the positive control well; the culture plate was placed in a 35°C constant temperature incubator for overnight culture, and the results were measured.

[0067] (3) Result determination: Determine the MIC (minimum drug concentration that can completely inhibit bacterial growth) of each group of Chinese medicine compositions. The bacterial growth test in the positive control wells must be significant. If multiple skipped wells occur, the results will not be reported and the test should be repeated.

[0068] The MIC test results of the Chinese medicine compositions of various embodiments and comparative examples are shown in Table 2.

[0069] Table 2 MIC determination results of Chinese medicine composition

[0070]

[0071] Experimental Example 2 Combined drug sensitivity test of Chinese medicine composition and antibiotics

[0072] The combined drug sensitivity test of the Chinese medicine composition and antibiotics was carried out using the diffusion method. The specific method is as follows:

[0073] (1) Activation of bacterial strains and preparation of bacterial solution: streak frozen Acinetobacter baumannii strains onto nutrient agar plates and culture at 37°C overnight; pick a single colony and inoculate into 10 ml nutrient broth liquid culture medium and culture at 37°C, 200 rpm, in a shaking incubator overnight for later use.

[0074] (2) Plate coating: dilute the bacterial solution from step (1) with sterile saline to 0.5 McFarland turbidity, use a sterile swab to dip the bacterial solution into the tube wall and squeeze it by rotation, remove the excess bacterial solution and evenly smear it on the surface of a nutrient agar plate (product of Guangzhou Dijing Company) for three times, rotate the plate 60 degrees each time, and finally circle around the plate twice to ensure that the entire surface of the culture medium is evenly coated.

[0075] (3) Use a sterile puncher to punch holes with a diameter of 6 mm on the solid culture medium coated with the bacterial solution; add the Chinese medicine composition solution of each embodiment and comparative example into the hole, with a concentration of 200 mg / ml, 80 μl / hole. Stick imipenem, minocycline, rifampicin, chloramphenicol and tetracycline sensitive paper sheets (products of OXOID) at appropriate distances around the Chinese medicine composition solution holes.

[0076] (4) Place the plate in a 35°C constant temperature incubator and continue to culture overnight, then measure the diameter of the inhibition zone.

[0077] The results showed that the Chinese medicine composition of Examples 1-3 had different degrees of synergistic effects with imipenem, minocycline, rifampicin, chloramphenicol and tetracycline on the inhibition of Acinetobacter baumannii. The results of the in vitro antibacterial experiment of the Chinese medicine composition of Example 1 with each antibiotic were as follows: Figure 1 shown.

[0078] Experimental Example 3 In vivo antibacterial experiment of Chinese medicine composition and its combination with antibiotics

[0079] The in vivo antibacterial experiment of the Chinese medicine composition and its combination with antibiotics was conducted according to the method reported in the reference ("Pharmacological Test Methodology of Traditional Chinese Medicine", editor-in-chief: Li Yikui).

[0080] Experimental materials: Kunming mice weighing 22±2g (purchased from Jinan Pengyue Experimental Animal Breeding Co., Ltd.); Acinetobacter baumannii clinical isolate 19101113; imipenem (Hangzhou Merck Pharmaceutical Co., Ltd., approval number: National Medicine Standard J20180060, production batch number: S040704); minocycline (Hanhui Pharmaceutical Co., Ltd., approval number: National Medicine Standard H20174081, production batch number: 19032011).

[0081] 1. Determination of bacterial LD90

[0082] (1) Pick a single colony of Acinetobacter baumannii from the plate and place it in 10 ml of nutrient broth and culture it overnight at 160 rpm and 35°C;

[0083] (2) Take 100 μl of the overnight culture solution and inoculate it into 10 ml of fresh nutrient broth. Cultivate at 160 rpm and 35°C until the OD value is 1.0;

[0084] (3) diluting the bacterial solution obtained in step (2) by 10 times in 7 gradients, inoculating nutrient agar plates, 100 μl / plate, and culturing at 35° C. overnight;

[0085] (4) Count the number of colonies on the plate and convert the bacterial solution concentration to 8.6×10 9 cfu / ml;

[0086] (5) Kunming mice were randomly divided into 7 groups, with 5 mice in each group; the bacterial solution obtained in step (2) was cultured from 2×10 9 cfu / ml, and then 5 dilutions were made into 5 concentrations; the bacterial solutions of different concentrations were injected intraperitoneally into the infected mice, 0.5 ml per mouse; the control group was injected with an equal amount of normal saline.

[0087] (6) The mortality of mice was recorded (Table 3). The median lethal dose was calculated by SPSS software BLISS method and was 1.975×10 8 cfu, 95% confidence interval is 1.139×10 8 -4.464×10 10 cfu.

[0088] Table 3 Determination of bacterial LD90

[0089] Group Inoculation amount (cfu / each) Number of mice Number of deaths (nearly) mortality rate(%) 1 <![CDATA[10 9 ]]> 5 5 100 2 <![CDATA[10 8 ]]> 5 2 40 3 <![CDATA[10 7 ]]> 5 1 20 4 <![CDATA[10 6 ]]> 5 0 0 5 <![CDATA[10 5 ]]> 5 0 0 6 <![CDATA[10 4 ]]> 5 0 0 7 0 5 0 0

[0090] 2. In vivo antibacterial experiment

[0091] The in vivo antibacterial experiment was conducted on the Chinese medicine composition of Examples 1-3, and the specific method is as follows:

[0092] (1) Kunming mice were randomly divided into 8 groups, namely, control group, high-dose Chinese medicine group, medium-dose Chinese medicine group, low-dose Chinese medicine group, imipenem group, medium-dose combined with imipenem treatment group, minocycline group, and medium-dose combined with minocycline treatment group, with 15 mice in each group.

[0093] (2) Dilute the overnight culture of Acinetobacter baumannii to a concentration of 5 LD90 (9.6×10 8 / ml), and mice were infected by intraperitoneal injection, 200μl / mouse.

[0094] (3) 4 hours before infection, the control group was intraperitoneally injected with 200 μl of normal saline, the high-dose Chinese medicine group, the medium-dose Chinese medicine group, and the low-dose Chinese medicine group were intraperitoneally injected with the corresponding doses shown in Table 4, the imipenem group was intraperitoneally injected with imipenem 11.34 mg / animal, the medium-dose combined with imipenem group was intraperitoneally injected with imipenem 11.34 mg / animal and the corresponding dose of the Chinese medicine composition, the minocycline group was intraperitoneally injected with minocycline 0.286 mg / animal, and the medium-dose combined with minocycline group was intraperitoneally injected with minocycline 0.286 mg / animal and the corresponding dose of the Chinese medicine composition; the injection volume was 200 μl; once a day, for 5 consecutive days. The first dose of minocycline was double dose (0.572 mg / animal).

[0095] (4) The death of mice was recorded, and the experimental results were analyzed by Fisher method using SPSS software. P = 0.025 (< 0.05).

[0096] The results are shown in Table 4, which show that the Chinese medicine composition of each embodiment can play a significant inhibitory effect on Acinetobacter baumannii in vivo, and effectively reduce the mortality rate of animals infected with Acinetobacter baumannii; at the same time, the Chinese medicine composition of each embodiment has a good synergistic effect with imipenem and minocycline.

[0097] Table 4 In vivo antibacterial effect of Chinese medicine composition and its combination with antibiotics

[0098]

[0099]

[0100] Although the present invention has been described in detail above with general descriptions and specific embodiments, it is obvious to those skilled in the art that some modifications or improvements may be made thereto based on the present invention. Therefore, these modifications or improvements made without departing from the spirit of the present invention all fall within the scope of protection claimed by the present invention.

Claims

1. A Chinese medicine composition for inhibiting Acinetobacter baumannii, characterized in that: The invention is composed of the following components in parts by weight: 1-10 parts of sappan wood extract, 1-3 parts of sanguisorba officinalis charcoal extract, 1-10 parts of rose flower extract, 1-5 parts of sansevieria scabra extract, 1-5 parts of madder charcoal extract, 1-5 parts of hawthorn extract and 1-5 parts of stir-fried tribulus terrestris extract.

2. The Chinese medicine composition according to claim 1, characterized in that: The Herba Cynanchums officinalis extract, the Herba Rubiae charcoal extract, the Herba Crataeguse extract and the Herba Tribuluse terrestris extract are water extracts; The preparation method of the water extract is as follows: put the blood scorching, madder charcoal, hawthorn or stir-fried tribulus in water and boil for 1-3 times, each time boiling for 30-60 minutes, and then combine the decoctions and dry them to a solid state; The mass ratio of material to liquid for each decoction is 1:(10-30).

3. Use of the Chinese medicine composition according to any one of claims 1 to 2 in the preparation of drugs for inhibiting Acinetobacter baumannii.

4. Use of the Chinese medicine composition according to any one of claims 1 to 2 in combination with antibiotics in the preparation of drugs for inhibiting Acinetobacter baumannii, characterized in that: The antibiotic is one or more selected from imipenem, minocycline, rifampicin, chloramphenicol and tetracycline.

5. A drug for inhibiting Acinetobacter baumannii, characterized in that: The invention is composed of the traditional Chinese medicine composition according to any one of claims 1 to 2 and one or more selected from imipenem, minocycline, rifampicin, chloramphenicol and tetracycline.

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