Airtight culture devices, rotating racks, and biological sample imaging equipment

Abstract

The embodiments of the present disclosure disclose an airtight culture device, a rotating rack, and a biological sample imaging device. The airtight culture device includes: a frame, a rotating shaft is installed at one end, and a back cover is installed at the back; an inner box body is embedded in the accommodating space formed by the frame and the back cover, and is used to accommodate the sample culture dish; a front cover is rotatably connected to the frame by means of the rotating shaft to realize the opening and closing of the airtight culture device; wherein, the inner box body is provided with grooves around it, and a sealing component is installed in the groove, and the front cover can be rotated by means of the rotating shaft until it is in close contact with the sealing component, and at this time, the culture device can form an airtight space. According to the embodiments of the present disclosure, not only can the sealing of the culture device be guaranteed to prevent external gas from entering and polluting the internal environment of the culture device, but also the concentration of gas components in the culture device can be accurately controlled during the sample culture process, supporting the observation of a large number of samples at one time.

Description

Technical Field

[0001] The present disclosure relates to the technical field of biological instruments, and in particular to an airtight culture device, a rotating rack and a biological sample imaging device. Background Art

[0002] In botanical scientific research, continuous observation of the temporal and spatial specificity of plant growth and development is an indispensable research activity. In the same study, it is often necessary to continuously observe, photograph or videotape a large number of samples in order to fully record the entire process of sample growth and control the variables within a reasonable range. Based on the above requirements, it is necessary to design an observation device that can accommodate a large number of samples, and a culture device that can be used for the observation device to accommodate a single sample. The existing culture device is difficult to observe a large number of samples at one time, and it is impossible to accurately control the concentration of gas components in the culture device during the sample culture process while ensuring the sealing of the culture device. That is, if the sealing of the culture device is guaranteed, it is difficult to accurately control the concentration of gas components in the culture device during the sample culture process; if the concentration of gas components in the culture device is accurately controlled, it is difficult to ensure the sealing of the culture device, which is easy to cause pollution to the internal environment of the culture device. Summary of the invention

[0003] In order to solve the problems in the related art, the embodiments of the present disclosure provide an airtight culture device, a rotating rack, and a biological sample imaging device.

[0004] In a first aspect, an airtight culture device is provided in an embodiment of the present disclosure.

[0005] Specifically, the airtight culture device comprises:

[0006] The frame has a rotating shaft at one end and a back cover at the back;

[0007] An inner box body is embedded in the accommodation space formed by the frame and the rear cover, and is used to accommodate the sample culture dish;

[0008] A front cover is rotatably connected to the frame via the rotating shaft to realize the opening and closing of the airtight culture device;

[0009] Among them, the inner box body is provided with grooves around it, and a sealing component is installed in the groove. The front cover can be rotated by the rotating shaft to be in close contact with the sealing component, and at this time, the culture device can form an airtight space.

[0010] Optionally, a groove is provided at the connection between the frame and the rotating shaft, and an elastic component is installed between the frame and the rotating shaft, so that the rotating shaft moves back and forth in the groove under the action of the elastic component.

[0011] Optionally, a first fastener is installed on the other side of the frame, i.e., the side opposite to the rotating shaft, and a second fastener is installed on the side of the front cover away from the rotating shaft. The first fastener and the second fastener are fastened together, and with the help of the fastening connection between the first fastener and the second fastener, the front cover can fit tightly with the sealing assembly to form an airtight space for the culture device.

[0012] Optionally, an air inlet and an air outlet are provided on the back of the inner box body, wherein the air inlet is connected to an external air pump for pumping external air into the culture device, and an air filter assembly is provided in the air path for pumping in air to filter bacteria in the input air so that the air pumped into the culture device is sterile air; and the air outlet is used to discharge the air in the culture device.

[0013] Optionally, a water storage tank is provided at the top of the frame near the air inlet hole for storing sterile water, and a water transfer hole is provided on the side wall of the water storage tank toward the interior of the inner box body for guiding the sterile water stored in the water storage tank to the inner box body. After the guided water evaporates, it is brought into the interior of the inner box body by the air pumped in through the air inlet hole, so as to increase the humidity of the air inside the inner box body.

[0014] Optionally, a first lighting assembly is installed on the top of the frame to provide a light source for the sample to be observed; and / or,

[0015] A second lighting assembly 48 is installed at the side end of the frame, between the inner box body and the external device, so as to change the direction of light felt by the sample to be observed.

[0016] Optionally, the light sources of the first lighting component and the second lighting component adopt an LED aluminum substrate, and multiple lamp beads are arranged on the LED aluminum substrate, and at least two of the white light, red light, far red light, blue light, ultraviolet light, and green light sources are combined and encapsulated in the lamp beads.

[0017] Optionally, a filter installation groove is provided on the inner side surface of the frame close to the back of the sample culture dish for installing a filter.

[0018] In a second aspect, a rotating frame is provided in an embodiment of the present disclosure.

[0019] Specifically, the rotating frame comprises:

[0020] Rotating platform;

[0021] A bracket body, fixed to the rotating platform, comprises a first bracket and a second bracket, wherein the second bracket is radially arranged, and the first bracket is fixed to the second bracket;

[0022] The airtight culture device as described in any one of the first aspect and the first to seventh implementations of the first aspect is detachably installed between two adjacent first brackets.

[0023] In a third aspect, a biological sample imaging device is provided in an embodiment of the present disclosure.

[0024] Specifically, the biological sample imaging device comprises:

[0025] an incubator having an observation window;

[0026] The rotating rack as described in the second aspect is arranged inside the incubator;

[0027] An imaging module is used to collect images of the sample to be observed inside the culture device on the rotating rack through the observation window.

[0028] According to the technical solution provided by the embodiment of the present disclosure, the culture device forms an airtight space by means of the grooves arranged around the inner box body, the sealing components installed in the grooves, and the close contact between the front cover and the sealing components after rotation. The above technical solution can not only ensure the sealing of the culture device and prevent the external gas from entering and polluting the internal environment of the culture device, but also can accurately control the concentration of gas components in the culture device during the sample culture process, supporting the observation of a large number of samples at one time.

[0029] It is to be understood that the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the present disclosure. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Other features, objectives and advantages of the present disclosure will become more apparent through the following detailed description of non-limiting embodiments in conjunction with the accompanying drawings. In the accompanying drawings:

[0031] Figure 1 A front view showing an airtight culture device according to an embodiment of the present disclosure;

[0032] Figure 2 A side view of an airtight culture device according to an embodiment of the present disclosure is shown;

[0033] Figure 3 A top view of an airtight culture device according to an embodiment of the present disclosure is shown;

[0034] Figure 4 A rear view of an inner box body according to an embodiment of the present disclosure is shown;

[0035] Figure 5 A schematic diagram showing the structure of the top of the frame according to an embodiment of the present disclosure;

[0036] Figure 6A schematic diagram showing the installation of a second lighting assembly according to an embodiment of the present disclosure is shown;

[0037] Figure 7 A schematic structural diagram of an LED aluminum substrate according to an embodiment of the present disclosure is shown;

[0038] Figure 8 A schematic diagram showing the structure of a rotating frame according to an embodiment of the present disclosure is shown;

[0039] Fig. 9 A schematic structural diagram of a biological sample imaging device according to an embodiment of the present disclosure is shown. DETAILED DESCRIPTION

[0040] Hereinafter, exemplary embodiments of the present disclosure will be described in detail with reference to the accompanying drawings so that those skilled in the art can easily implement them. In addition, for the sake of clarity, parts not related to the description of the exemplary embodiments are omitted in the accompanying drawings.

[0041] In the present disclosure, it should be understood that terms such as "include" or "have" are intended to indicate the presence of features, numbers, steps, behaviors, components, parts, or a combination thereof disclosed in the present specification, and are not intended to exclude the possibility that one or more other features, numbers, steps, behaviors, components, parts, or a combination thereof exist or are added.

[0042] It should also be noted that, in the absence of conflict, the embodiments and features in the embodiments of the present disclosure may be combined with each other. The present disclosure will be described in detail below with reference to the accompanying drawings and in combination with the embodiments.

[0043] In botanical scientific research, continuous observation of the temporal and spatial specificity of plant growth and development is an indispensable research activity. In the same study, it is often necessary to continuously observe, photograph or videotape a large number of samples in order to fully record the entire process of sample growth and control the variables within a reasonable range. Based on the above requirements, it is necessary to design an observation device that can accommodate a large number of samples, and a culture device that can be used for the observation device to accommodate a single sample. The existing culture device is difficult to observe a large number of samples at one time, and it is impossible to accurately control the concentration of gas components in the culture device during the sample culture process while ensuring the sealing of the culture device. That is, if the sealing of the culture device is guaranteed, it is difficult to accurately control the concentration of gas components in the culture device during the sample culture process; if the concentration of gas components in the culture device is accurately controlled, it is difficult to ensure the sealing of the culture device, which is easy to cause pollution to the internal environment of the culture device.

[0044] In order to solve the problems in the related art, the inventors have designed an airtight culture device, which includes: a frame, with a rotating shaft installed at one end and a back cover installed at the back; an inner box body, embedded in the accommodating space formed by the frame and the back cover, for accommodating the sample culture dish; a front cover, rotatably connected to the frame by means of the rotating shaft, so as to realize the opening and closing of the airtight culture device; wherein the inner box body is provided with grooves around it, and a sealing component is installed in the groove, and the front cover can be rotated by means of the rotating shaft until it is in close contact with the sealing component, and at this time, the culture device can form an airtight space. The airtight culture device can not only ensure the sealing of the culture device and prevent external gas from entering and polluting the internal environment of the culture device, but also support the observation of a large number of samples at one time.

[0045] Figure 1 A front view showing an airtight culture device according to an embodiment of the present disclosure, Figure 2 A side view of an airtight culture device according to an embodiment of the present disclosure is shown, Figure 3 FIG. 2 shows a top view of an airtight culture device according to an embodiment of the present disclosure. Figure 1 , Figure 2 and Figure 3 As shown, the airtight culture device comprises:

[0046] The frame 101 has a rotating shaft 62 mounted on one side and a rear cover (not shown) mounted on the back;

[0047] The inner box body 59 is embedded in the accommodation space formed by the frame 101 and the rear cover, and is used to accommodate the sample culture dish 40;

[0048] The front cover 57 is rotatably connected to the frame 101 by means of the rotating shaft 62 to realize the opening and closing of the airtight culture device;

[0049] The inner box body 59 is provided with grooves 60 around it, and a sealing component is installed in the groove 60. The front cover 57 can be rotated by the rotating shaft 62 to be in close contact with the sealing component, and the culture device can form an airtight space.

[0050] The sample culture dish 40 is used to hold the sample to be observed. The sample to be observed may be, for example, a plant seedling sample. In this embodiment, the sample culture dish 40 may be sterilized by ethylene oxide or cobalt-60 radiation before use, and a high-temperature sterilized culture medium is added, and the culture medium contains the plant seedling sample.

[0051] In one embodiment of the present disclosure, the frame 101 is a hollow structure to form a receiving space with the back cover to accommodate the inner box body 59 .

[0052] In one embodiment of the present disclosure, the back cover is made of a transparent material to facilitate observation and photography of the internal conditions of the culture device.

[0053] In one embodiment of the present disclosure, a groove 109 is provided at the connection between the frame 101 and the rotating shaft 62, and an elastic component 110 is installed between the frame 101 and the rotating shaft 62, so that the rotating shaft 62 moves forward and backward in the groove 109 under the action of the elastic component 110. The elastic component 110 may be, for example, a spring.

[0054] In one embodiment of the present disclosure, the sealing component is a sealing component with sealing performance and made of an elastic material, for example, the sealing component may be a rubber ring.

[0055] In one embodiment of the present disclosure, a first fastener 61 or 113 is installed on the other side of the frame 101, that is, the side opposite to the rotating shaft 62, and a second fastener 102 is installed on the side of the front cover 57 away from the rotating shaft 62. The first fastener 61 or 113 can be fastened to the second fastener 102. With the fastening connection between the first fastener 61 or 113 and the second fastener 102, the front cover 57 can be tightly fitted with the sealing component to form an airtight space for the culture device. The first fastener 61 or 113 can be, for example, a rotatable wrench, and the second fastener 102 can be, for example, a hook, a circle or other attachable component that can be fastened to the rotatable wrench. When the rotatable wrench is rotated, the rotatable wrench can buckle the attachable component to present a fixed connection state.

[0056] Based on the above technical solution, when it is necessary to realize an internal airtight culture device, the front cover 57 can be first stretched outward with the rotating shaft 62 as the force point, that is, the front cover 57 is stretched toward the outside of the culture device to overcome the force of the elastic component 110. When the front cover 57 is rotated to contact the sealing component installed around the inner box body 59, the first fastener 61 or 113 is fastened to the second fastener 102 so that the front cover 57 is tightly fitted with the sealing component. At this time, the first fastener 61 or 113 is fastened to the second fastener 102, and the force of the elastic component 110 can be used to pull the front cover 57 tightly toward the frame 101 and the inner box body 59, ensuring that the surfaces of the front cover 57 and the inner box body 59 form a closed space through the sealing component.

[0057] In addition, since the culture device is provided with a front cover to form a closed space, the sample culture dish 40 can be placed in the culture device without its own upper cover, and directly placed in the inner box body 59 of the culture device. Furthermore, since the upper cover of the sample culture dish 40 is omitted, the condensation on the upper cover of the sample culture dish 40 during the long-term culture of the sample can be effectively avoided to affect the imaging of the sample to be observed, and the detail resolution of the imaging of the sample to be observed can also be significantly improved.

[0058] In one embodiment of the present disclosure, a light-transmitting observation window is also provided on the front cover 57, so as to observe the sample to be observed placed in the sample culture dish 40 through the observation window. Furthermore, in one embodiment of the present disclosure, the observation window is equipped with double-sided coated glass 58 to enhance the transmittance of the visible light band and the 940nm infrared light band.

[0059] Figure 4 A rear view of the inner box body according to an embodiment of the present disclosure is shown, Figure 4 As shown, in one embodiment of the present disclosure, the back of the inner box body 59 is provided with an air inlet 63 and an air outlet 64, wherein the air inlet 63 is connected to an external air pump for pumping external air into the culture device, and an air filter assembly, such as a filter membrane with a pore size of 0.22 μm (not shown in the figure), may be provided in the air path for pumping in air to filter microorganisms in the input air, so that the air pumped into the culture device is sterile air. The air outlet 64 is used to discharge the air in the culture device.

[0060] During operation, air is continuously pumped in through the air inlet 63, and air is discharged through the air outlet 64 to relieve pressure, so that the air pressure inside the culture device is always slightly greater than that outside, thereby preventing the entry of sterile air from the outside, and ensuring that the inside of the culture device is always in a sterile environment. In addition, since the air pump can quickly exchange the air components inside the culture device with the air components outside, and the sample to be observed is directly exposed to the air inside the culture device, when it is necessary to adjust the concentration of the gas components inside the culture device with the help of other control equipment, the sample to be observed can quickly feel the change in the concentration of the gas components.

[0061] It should be noted that the gas flow rate pumped into the culture device can be set according to the needs of actual application. For example, for the plant sample to be observed with a culture medium, the gas flow rate pumped into the culture device should be controlled at about 30-100 mL / min, because if the gas flow rate is too high, it is easy to cause the culture medium to dry up, and if the gas flow rate is too low, it is not conducive to the rapid balance between the internal environment of the culture device and the external environment.

[0062] Figure 5 A schematic diagram showing the structure of the top of the frame according to an embodiment of the present disclosure is shown in FIG. Figure 5 As shown, in one embodiment of the present disclosure, a water storage tank 65 is provided at the top of the frame 101 near the air inlet 63 for storing sterile water, wherein a water delivery hole 114 is provided on the side wall of the water storage tank 65 toward the interior of the inner box body 59, for guiding the sterile water stored in the water storage tank to the inner box body 59; after the volatilization of the guided water, it can be brought into the interior of the inner box body 59 by the air pumped in by the air inlet 63, so as to increase the humidity of the air inside the inner box body 59, slow down the evaporation of water required for the sample culture medium to be observed, and prevent the evaporation of water and the drying up of the sample culture medium to be observed due to long-term ventilation.

[0063] In one embodiment of the present disclosure, the water storage tank 65 is also equipped with a water tank cover 66, for example, Figure 5 As shown in the T-shaped water tank cover, after the water storage tank 65 is injected with sterile water, the water tank cover 66 can be covered to prevent the sterile water in the water storage tank 65 from being contaminated by microorganisms in the outside air.

[0064] In one embodiment of the present disclosure, a first lighting assembly 44 may be installed at the top of the frame 101 to provide a light source for the sample to be observed.

[0065] In one embodiment of the present disclosure, the culture device may further include at least two support members 50, the support members 50 are relatively arranged on both sides of the top of the frame 101, and a slot is provided on the top of the support member 50, so that the two ends of the first lighting component 44 can be pushed into the slot and then fixed to the top of the frame 101 with the help of the support member 50. Taking the case where there are two support members 50 as an example, the two support members 50 are respectively fixed at two right angles at the top of the frame 101, and a slot is provided on the top of the opposite side of the two support members 50, and the slot can cooperate with the protrusions at both ends of the first lighting component 44 to detachably fix the first lighting component 44 between the two support members 50. In addition, through the slot design of the support member 50, the first lighting component 44 can be quickly disassembled and assembled to meet the different requirements for illumination at different time periods or different test items during the sample culture process. In this embodiment, the frame 101 may be U-shaped, that is, only the two ends of the top of the frame 101 are connected to the first lighting component 44 through the support member 50, and the other parts of the top of the frame 101 are hollow structures, so that the light emitted by the first lighting component 44 can directly irradiate the culture dish 20 and the sample to be observed.

[0066] Considering that the existing equipment cannot guarantee that each plant sample in a large-scale experiment can be illuminated with the same distance, angle and intensity. In the process of plant growth, light is a very important influencing factor. Changes in the intensity, angle and wavelength of light directly affect the growth of plant samples. Therefore, in one embodiment of the present disclosure, a second lighting assembly 48 can be installed at the side end of the frame 101, such as the side end of the frame 101 close to the shaft 62, between the inner box body 59 and the external device, such as Figure 6 As shown, the light direction felt by the sample to be observed is changed, which can be used to study the phototropism of plants. The second lighting assembly 48 can be fixed to the frame 101 by screws or buckles.

[0067] In one embodiment of the present disclosure, the first lighting assembly 44 and the second lighting assembly 48 may both include a light source and a light diffuser, and the light diffuser is disposed on the light propagation path of the light source. In order to prevent differences in lighting effects at different positions of the lighting assembly, which may cause errors in experimental results, light diffusers may be installed on the lighting assembly so that light can be evenly irradiated onto the sample to be observed, thereby reducing experimental errors caused by uneven light.

[0068] In one embodiment of the present disclosure, Figure 7 As shown, the light source of the first lighting component 44 and the second lighting component 48 can adopt an LED aluminum substrate 43, wherein a plurality of lamp beads are arranged on the LED aluminum substrate. The lamp beads are combined and packaged with at least two of white light, red light, far red light, blue light, ultraviolet light, and green light sources, and can provide light sources of far red light, red light, green light, blue light, white light, ultraviolet light, etc. with different wavelengths, and the control circuit of the device can control the on-off and light intensity of each light separately. Multiple monochromatic lights, especially wavelengths that may be turned on at the same time such as red light, far red light, blue light, etc., are combined and packaged in a lamp bead to avoid differences in the position and direction of the light source when different wavelengths are turned on, thereby affecting the consistency of the experimental conditions. For white light and each monochromatic light that will not be turned on at the same time, they can be divided into two groups of lamp beads for packaging and arranged alternately to meet the requirements of greater output power. A LED light source for cultivation can simultaneously control at least 4 different color light sources.

[0069] In one embodiment of the present disclosure, in order to allow the light emitted by the lighting assembly to pass through the inner box body 59, the inner box body 59 is made of a transparent material, such as a transparent acrylic material, etc.

[0070] In one embodiment of the present disclosure, a filter installation groove is provided on the inner side surface of the frame 101 near the back of the sample culture dish 40 for installing a filter. Since the inner box body 59 is made of a transparent material, when a large number of samples need to be studied and photographed under lighting conditions, the light emitted by the lighting component of the culture device is likely to interfere with the samples to be observed in other adjacent or close culture devices, or the light emitted by the lighting component is reflected by the material of the frame 101 inside the culture device, so that the light received by the sample to be observed is not only in the direction of the lighting component, resulting in errors in the study of the phototropism of the sample. Among them, the filter can be selected from different types according to the needs of the actual application. For example, the filter can be an ultraviolet filter, a visible filter, an infrared filter, etc.

[0071] In one embodiment of the present disclosure, fixing holes may be opened at both ends of the frame 101 or the back surface of the back cover to connect with the rotating frame, wherein the rotating frame may be connected to multiple culture devices to achieve simultaneous observation of a large number of samples to be observed.

[0072] Figure 8 A schematic structural diagram of a rotating frame according to an embodiment of the present disclosure is shown.

[0073] like Figure 8 As shown, the rotating frame includes:

[0074] Rotating platform 14;

[0075] A support body, fixed to the rotating platform 14, includes a first support 111 and a second support 112, wherein the second support 112 is radially arranged, and the first support 111 is fixed to the second support 112; and

[0076] The airtight culture device as described above can be detachably installed between two adjacent first brackets 111 .

[0077] According to the embodiment of the present disclosure, at least one rack fixing member 12 is fixed on each first bracket 111. The airtight culture device can be fixed between the rack fixing members 12 of two adjacent first brackets 111. For example, at least two mounting holes are respectively provided on both sides of the rack fixing member 12, which cooperate with the fixing holes of the airtight culture device, and the airtight culture device is fixed by screws, nuts and other parts. It should be noted that the rotating rack can be used to fix a variety of culture devices. In addition to the airtight culture device 38 described above, it can also be used for other types of culture devices, such as a standard small vertical culture device 36, a large horizontal culture dish holder 37, a gravity culture dish holder 39, and the like. Among them, the airtight culture device 38, the standard small vertical culture device 36, and the gravity culture dish holder 39 can occupy one rack position on the rotating rack, and the large horizontal culture dish holder 37 can occupy two rack positions on the rotating rack.

[0078] In one embodiment of the present disclosure, an electrical socket interface 13 may also be provided on the first bracket 111 , and the wiring of the lighting assembly of the airtight culture device may be connected to the electrical socket interface 13 .

[0079] The rotating rack provided in the embodiment of the present disclosure can fix multiple culture devices on several adjacent installation positions through universal rack fixing parts. Driven by the rotating platform, different culture devices can be respectively aligned with imaging devices (such as CCD cameras or CMOS cameras), so that imaging analysis of samples to be observed in multiple culture devices can be conveniently performed at the same time, thereby improving sample analysis throughput and improving sample analysis efficiency.

[0080] Fig. 9 A schematic structural diagram of a biological sample imaging device according to an embodiment of the present disclosure is shown.

[0081] like Fig. 9 As shown, the biological sample imaging device comprises:

[0082] An incubator 4, on which an observation window is provided;

[0083] The rotating frame 5 is disposed inside the incubator 4; and

[0084] The imaging module 3 is used to collect images of the sample to be observed inside the culture device on the rotating frame 5 through the observation window.

[0085] In an embodiment of the present disclosure, the biological sample imaging device may further include a housing 1 and an optical seismic isolation platform 2 , wherein the rotating frame 5 may pass through an opening at the bottom of the incubator 4 and be fixed on the optical seismic isolation platform 2 .

[0086] As described above, the sample to be observed can be a plant sample, especially a plant seedling sample. The biological sample imaging device provided in the embodiment of the present disclosure can be a plant seedling imaging device, which is used to analyze the growth status of plant seedlings. The culture device can be installed on the rotating frame 5 to fix the sample culture dish. The incubator 4 can flexibly adjust the environment for the growth of plant seedlings, including temperature, gas component concentration, etc., to meet the control requirements of various experimental condition variables. The observation window on the side wall of the incubator 4 allows the imaging module 3 outside the incubator 4 to collect image data of the plant seedlings inside the incubator 4, which is used to study changes in the growth of plant seedlings.

[0087] The above description is only a preferred embodiment of the present disclosure and an explanation of the technical principles used. Those skilled in the art should understand that the scope of the invention involved in the present disclosure is not limited to the technical solution formed by a specific combination of the above technical features, but should also cover other technical solutions formed by any combination of the above technical features or their equivalent features without departing from the inventive concept. For example, the above features are replaced with the technical features with similar functions disclosed in the present disclosure (but not limited to) by each other.

Claims

1. An airtight culture device, characterized in that: include: The frame has a rotating shaft at one end and a back cover at the back; An inner box body is embedded in the accommodation space formed by the frame and the rear cover and is used to accommodate the sample culture dish; A front cover is rotatably connected to the frame by means of the rotating shaft to realize the opening and closing of the airtight culture device; the front cover is also provided with a light-transmitting observation window; The inner box body is provided with grooves around its periphery, and a sealing component is installed in the groove. The front cover can be rotated by the rotating shaft until it is in close contact with the sealing component, and at this time, the culture device can form an airtight space; An air inlet and an air outlet are provided on the back of the inner box body, wherein the air inlet is connected to an external air pump for pumping external air into the culture device, and an air filter assembly is provided in the air path for pumping in air to filter bacteria in the input air so that the air pumped into the culture device is sterile air; the air outlet is used to discharge the air in the culture device.

2. The airtight culture device according to claim 1, characterized in that: A groove is provided at the connection between the frame and the rotating shaft, and an elastic component is installed between the frame and the rotating shaft, so that the rotating shaft moves forward and backward in the groove under the action of the elastic component.

3. The airtight culture device according to claim 2, characterized in that: A first fastener is installed on the other side of the frame, i.e., the side opposite to the rotating shaft, and a second fastener is installed on the side of the front cover away from the rotating shaft. The first fastener is fastened to the second fastener, and with the help of the fastening connection between the first fastener and the second fastener, the front cover can fit tightly with the sealing assembly to form an airtight space for the culture device.

4. The airtight culture device according to any one of claims 1 to 3, characterized in that: The frame is provided with a water storage tank at the top near the air inlet for storing sterile water. A water delivery hole is provided on the side wall of the water storage tank toward the interior of the inner box body for directing the sterile water stored in the water storage tank to the inner box body. After the water is volatilized, it is brought into the interior of the inner box body by the air pumped in through the air inlet, so as to increase the humidity of the air inside the inner box body.

5. The airtight culture device according to claim 4, characterized in that: A first lighting assembly is installed on the top of the frame to provide a light source for the sample to be observed; and / or, A second lighting assembly is installed between the side end of the frame, the inner box body and the external device to change the direction of light felt by the sample to be observed.

6. The airtight culture device according to claim 5, characterized in that: The light sources of the first lighting component and the second lighting component adopt an LED aluminum substrate, on which a plurality of lamp beads are arranged, and at least two of white light, red light, far red light, blue light, ultraviolet light, and green light sources are combined and encapsulated in the lamp beads.

7. The airtight culture device according to any one of claims 1-3, 5-6, characterized in that: The inner side surface of the frame close to the back of the sample culture dish is provided with a filter installation groove for installing the filter.

8. A rotating frame, characterized in that: include: Rotating platform; A bracket body, fixed to the rotating platform, comprises a first bracket and a second bracket, wherein the second bracket is radially arranged, and the first bracket is fixed to the second bracket; The airtight culture device according to any one of claims 1 to 7 is detachably installed between two adjacent first brackets.

9. A biological sample imaging device, characterized in that: include: an incubator having an observation window; The rotating rack according to claim 8, arranged inside the incubator; An imaging module is used to collect images of the sample to be observed inside the culture device on the rotating rack through the observation window.

Images