Laboratory Animal Nose Dropper

Abstract

The invention relates to the field of veterinary medicine. The invention aims to solve the problem that the current experimental animal nasal dropper causes the drug liquid to overflow and damage the animal's nasal cavity. The nasal dropper of the invention comprises an injection tube and a latex capsule bubble, and the latex capsule bubble is sleeved on the injection tube. The invention adopts the latex capsule bubble as the animal's nasal cavity expansion component, blows it up by the latex capsule bubble inflation method of the soft part, and then supports the nasal cavity, so that the nasal drops can smoothly enter the animal's nasal cavity. The latex capsule bubble is tightly fitted with the inner wall of the nasal cavity in the circumferential direction to prevent the nasal drops from overflowing and causing the loss of the drug amount. Moreover, compared with the components made of metal and other materials, it will not damage the nasal cavity. The invention is applied to the field of experimental animals.

Description

Technical Field

[0001] The invention relates to the field of veterinary medicine, in particular to a nose dropper for experimental animals. Background Art

[0002] There are many ways to administer drugs during animal experiments, and nasal drops are a common method of administration. Currently, there is no special device for nasal drops, and existing nasal droppers are prone to cause drip overflow, or stretching the nasal cavity can easily cause nasal damage to animals. Summary of the invention

[0003] The purpose of the present invention is to solve the problem that the current experimental animal nose dropper causes liquid medicine to overflow and damages the animal's nasal cavity, and to provide an experimental animal nose dropper.

[0004] The experimental animal nose dropper of the present invention comprises an injection tube and a latex capsule bubble;

[0005] The injection tube is composed of an injection tube body, an air inlet push rod and a liquid inlet push rod; an air inlet groove is arranged on the outer periphery of the injection tube body along its circumference, and both ends of the air inlet groove are open, and one end of the air inlet push rod is placed in the air inlet groove; one end of the liquid inlet push rod is placed in the tube cavity of the injection tube, and the push handle of the liquid inlet push rod drives the push handle of the air inlet push rod to move forward together when advancing;

[0006] The front end of the emulsion capsule bubble is sleeved on the air inlet groove of the head of the injection tube body, and the air inlet is connected with the air outlet of the air inlet groove;

[0007] The front end of the injection tube is provided with a needle tube, the liquid inlet of the needle tube is connected with the liquid outlet of the injection tube body, the liquid outlet passes through the emulsion capsule bubble and is located outside the emulsion capsule bubble, and an air outlet is opened on the emulsion capsule bubble near the injection tube liquid outlet.

[0008] Furthermore, the experimental animal nasal dropper is also provided with a limit screw and a scale indicator rod; the outer wall of the injection tube body is engraved with scales, and the handle of the air intake push rod is connected to the front end of the limit screw and is provided with a scale indicator rod, and the front end of the scale indicator rod indicates the scale on the outer wall; the scale indicator rod matches the outer wall of the injection tube body.

[0009] Furthermore, a plurality of air outlet blocking beads are arranged in the emulsion capsule bubble.

[0010] Furthermore, the front and rear ends of the latex capsule bubble are open, the needle tube head passes through the front end opening of the latex capsule bubble, and the outer wall is pasted together with the latex capsule bubble, the rear end opening of the latex capsule bubble is sleeved on the outer wall of the air outlet of the air inlet groove and pasted together with it, and a plurality of air outlet blocking beads are placed in the latex capsule bubble.

[0011] Furthermore, the diameter of the air outlet blocking bead is larger than the diameter of the air outlet and the diameter of the air outlet of the air inlet groove.

[0012] Furthermore, the push handle of the air inlet push rod is opened in the middle, and the end of the liquid inlet push rod passes through the opening of the push handle of the air inlet push rod and is placed in the injection tube body.

[0013] Furthermore, the experimental animals are mice.

[0014] The present invention has the following beneficial effects:

[0015] The present invention adopts a milk capsule bubble as an animal nasal cavity expansion component, and after the milk capsule bubble is blown up by the soft part of the milk capsule bubble inflating mode, the nasal cavity is supported, so that the nasal drops can smoothly enter the animal nasal cavity. The milk capsule bubble is closely fitted with the inner wall of the nasal cavity circumferentially to prevent the nasal drops from overflowing, resulting in a loss of medicine, and compared with components made of metal and other materials, the nasal cavity will not be damaged. More importantly, the front end of the milk capsule bubble of the present invention is provided with an air outlet, and the direction of the gas discharged from the air outlet is the same as the direction of the nasal drops entering the nasal cavity. By controlling the opening amount, the gas is blown out while the milk capsule bubble is not rapidly reduced, ensuring that the milk capsule bubble is always closely fitted with the inner wall of the nasal cavity circumferentially when the nasal drops are injected. Moreover, the blown gas will push the nasal drops into the nasal cavity to reduce outflow. The present invention arranges an air inlet groove in the circumferential direction of the outer periphery of the injection tube, and arranges an air inlet push rod therein, and the air inlet push rod is pushed by the liquid inlet push rod to push the gas into the emulsion capsule bubble, and the emulsion capsule bubble expands rapidly while the nasal drops also enter the nasal cavity together, and since the air inlet groove and the cavity of the injection tube are similar in length, the liquid inlet push rod and the air inlet push rod reach the bottom almost at the same time, and therefore, after the nasal drops almost completely enter the nasal cavity, the gas entering the emulsion capsule bubble is almost completed, and the emulsion capsule bubble is gradually reduced at this time, thereby completing the smooth dripping of the nasal drops and preventing the nasal drops from overflowing.

[0016] The solution of the present invention can effectively solve the problem that the nasal dropper for experimental animals causes liquid medicine to overflow and damages the nasal cavity of the animals, and has a simple structure and strong practicality. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 This is a schematic diagram of the structure of the experimental animal nose dropper of the present invention;

[0018] Figure 2 The comparison photos of the experimental animal mice dripping nose before and after the nose dripping are taken in Example 1; wherein, the left picture in each group of pictures is before the nose dripping, and the right picture is after the nose dripping;

[0019] Figure 3 The following are HE staining images of the blank group and the infected group of mice after the nasal dripping of the experimental animal mice in Example 1. DETAILED DESCRIPTION

[0020] Those skilled in the art will appreciate that the above-mentioned embodiments are specific examples for implementing the present invention, and in actual applications, various changes may be made thereto in form and detail without departing from the spirit and scope of the present invention.

[0021] In order to make the objectives, technical solutions and advantages of the embodiments of the present invention more clearly understood, the spirit of the contents disclosed in the present invention will be described in detail below. After understanding the embodiments of the contents of the present invention, any technician in the relevant technical field can change and modify the techniques taught by the contents of the present invention without departing from the spirit and scope of the contents of the present invention.

[0022] The exemplary embodiments of the present invention and the description thereof are used to explain the present invention, but are not intended to limit the present invention.

[0023] Example 1

[0024] The experimental animal nose dropper of this embodiment is characterized by comprising an injection tube 1 and a latex capsule bubble 2;

[0025] The syringe 1 is composed of a syringe body 11, an air inlet push rod 12 and a liquid inlet push rod 13; an air inlet groove 5 is arranged on the outer periphery of the syringe body 11 along its circumference, and both ends of the air inlet groove 5 are open, and one end of the air inlet push rod 12 is placed in the air inlet groove 5; one end of the liquid inlet push rod 13 is placed in the lumen of the syringe 1, and the push handle of the liquid inlet push rod 13 drives the push handle of the air inlet push rod 12 to advance together when advancing; the front end of the emulsion capsule bubble 2 is sleeved on the air inlet groove 5 of the head of the syringe body 11, and the air inlet is connected to the air outlet of the air inlet groove 5;

[0026] A needle tube 14 is provided at the front end of the injection tube 1. The liquid inlet of the needle tube 14 is connected to the liquid outlet of the injection tube body 11. The liquid outlet passes through the emulsion capsule bubble 2 and is located outside the emulsion capsule bubble 2. An air outlet 21 is opened on the emulsion capsule bubble 2 near the liquid outlet of the injection tube 1.

[0027] The experimental animal nose dropper is also provided with a limit screw 3 and a scale indicator rod 4; the outer tube wall of the injection tube body 11 is engraved with scales, and the handle of the air inlet push rod 12 is connected to the front end of the limit screw 3 and is provided with a scale indicator rod 4, and the front end of the scale indicator rod 4 indicates the scale on the outer tube wall; the scale indicator rod 4 matches the outer tube wall of the injection tube body 11. The emulsion capsule bubble 2 is also provided with a plurality of outlet blocking beads.

[0028] The emulsion capsule bubble 2 is opened at both ends, the head of the needle tube 14 passes through the front end opening of the emulsion capsule bubble 2, and the outer wall is pasted together with the emulsion capsule bubble 2, the rear end opening of the emulsion capsule bubble 2 is sleeved on the outer wall of the air outlet of the air inlet groove 5 and pasted together with it, and a plurality of air outlet blocking beads are placed in the emulsion capsule bubble 2.

[0029] The diameter of the air outlet blocking bead is larger than the diameter of the air outlet 21 and the air outlet diameter of the air inlet groove 5 .

[0030] The push handle of the air inlet push rod 12 is opened in the middle, and the end of the liquid inlet push rod 13 passes through the opening of the push handle of the air inlet push rod 12 and is placed in the injection tube body 11.

[0031] The nose dropper of this embodiment was used to perform nose drops on mice, and the nose drop effect was observed. The results showed that no nose drop overflow was found, and no nasal cavity damage occurred.

[0032] 1 Materials and methods

[0033] 1.1 Main test instruments

[0034] Experimental animal nose dropper of Example 1

[0035] 1.2 Animals

[0036] Twenty BALB / c mice weighing 18-22 g, half male and half female, were provided by the Department of Experimental Animal Science, Harbin Medical University, license number SCXK(Black)2019-001.

[0037] 1.3 Main Reagents

[0038] The standard strain of Mycoplasma pneumoniae (ATCC15531) was purchased from American Type Culture Collection, cultured in fetal bovine serum for 24 hours, and then grown in PPLO medium containing 20% ​​fetal bovine serum and 10% yeast, and passaged every 7 days. PPLO medium (Becton, Dickinson and Company, USA); yeast (Angel Yeast Co., Ltd.); fetal bovine serum (Gibco, USA); hematoxylin and eosin stain (Nanjing Jiancheng Company); physiological saline.

[0039] 1.4 Main instruments and equipment

[0040] KD-TS3A automatic tissue dehydrator (Zhejiang Jinhua Kedi Instrument Equipment Co., Ltd.); KD-BM biological tissue embedding machine (Zhejiang Jinhua Kedi Instrument Equipment Co., Ltd.); RM2235 tissue slicer (German Leica); BX4-1 biological microscope (OLYMPUS); MDF-382E-80℃ low-temperature refrigerator (Japan SANYO); ST-16R low-temperature high-speed centrifuge (ThermoFisher Scientific, USA); LXJ-n ordinary low-speed centrifuge (Shanghai Medical Analytical Instrument Factory); 20uL, 200uL, 1000uL micropipette (Eppendoff); BP211D analytical balance (Sartorius).

[0041] 1.5 Methods

[0042] 1.5.1 Animal model was established mainly by nose dropper: 20 BALB / c mice weighing 20-22g were randomly divided into 2 groups, 10 mice in infection group (infection with Mycoplasma pneumoniae) and 10 mice in blank group. The mice in infection group were anesthetized with ether, and 20uL10CCU / mL Mycoplasma pneumoniae fluid was sucked by the experimental animal nose dropper. The mice were caught and injected into the nasal cavity through the mouse nose dropper for 3 consecutive days. The blank group was inhaled with the same volume of MP liquid culture medium by the experimental animal nose dropper. After the end, the mice were killed by myelotomy, and the lung tissue was separated. The left lung tissue was fixed with 10% formaldehyde for HE staining. The experiment was repeated 3 times.

[0043] 1.5.2HE staining: the most common method for detecting changes in tissue structure. The lung tissue is fixed in 10% formaldehyde for more than 48 hours, dehydrated, transparent, embedded, sliced, dewaxed, hydrated, stained with hematoxylin and eosin, dehydrated, and sealed with neutral gum. HE staining of lung tissue sections dyes the cell nucleus blue, and the cytoplasm, connective tissue and muscle are all dyed red to varying degrees. The pathological changes of lung tissue are observed under an optical microscope. The pathological score ranges from 0 (no change) to 16 (maximum change), and is rated from 1 to 4 points from mild to severe according to bronchiolitis, perivasculitis, interstitial pneumonia and alveolitis.

[0044] 2 Results

[0045] 2.1 During the nasal drip process, no liquid medicine flowed out, ensuring that all the medicine was injected into the nasal cavity; after the nasal drip, the nasal cavity of the mouse was observed, and except for drug irritation, no damage was caused to the nasal cavity of the mouse. Figure 2 .

[0046] 2.2 Pathological examination of lung tissue

[0047] HE staining showed that the lung tissue of mice in the blank group had no abnormal changes, the structure was clear, the alveolar cavity was intact and arranged regularly, and there was no inflammatory cell infiltration. In the infected group, the epithelial cells in the lung tissue of mice fell off, the bronchial space was thickened, there were excessive red blood cells in the alveolar wall capillaries, a large number of inflammatory cells infiltrated the alveolar wall and the septum, the alveolar wall collapsed, and focal atelectasis may occur. Figure 3 .

[0048] 3 Discussions

[0049] The experimental results show that the use of experimental animal nose droppers to drip models on mice, HE staining proves that the modeling is successful, indicating that the drug enters the mouse lungs to infect them, and does not damage the nasal cavity, greatly reducing the impact of other objective factors on the modeling during the modeling period. The experimental animal nose dropper plays a great role in the process of experimental animal nose drop modeling. The drug arrives accurately and accurately without error, minimizing the damage to the experimental animals, and does not affect the external factors of the objective environment of the experimental animals.

Claims

1. Experimental animal nose dropper, characterized in that It comprises an injection tube (1) and a milk capsule bubble (2); The injection tube (1) is composed of an injection tube body (11), an air inlet push rod (12) and a liquid inlet push rod (13); an air inlet groove (5) is arranged along the circumference of the outer periphery of the injection tube body (11), the air inlet groove (5) is open at both ends, and one end of the air inlet push rod (12) is placed in the air inlet groove (5); one end of the liquid inlet push rod (13) is placed in the tube cavity of the injection tube (1), and the push handle of the liquid inlet push rod (13) drives the push handle of the air inlet push rod (12) to be pushed together when being pushed; The front end of the emulsion capsule bubble (2) is sleeved on the air inlet groove (5) at the head of the injection tube body (11), and the air inlet is connected to the air outlet of the air inlet groove (5); The front end of the injection tube (1) is provided with a needle tube, the liquid inlet of the needle tube is connected with the liquid outlet of the injection tube body (11), the liquid outlet passes through the emulsion capsule bubble (2) and is located outside the emulsion capsule bubble (2), and an air outlet (21) is provided on the emulsion capsule bubble (2) near the liquid outlet of the injection tube (1); a plurality of air outlet blocking beads are also provided in the emulsion capsule bubble (2); the front and rear ends of the emulsion capsule bubble (2) are open, the needle tube head passes through the front end opening of the emulsion capsule bubble (2), and the outer wall is adhered to the emulsion capsule bubble (2), the rear end opening of the emulsion capsule bubble (2) is sleeved on the outer wall of the air outlet of the air inlet groove (5) and adhered to it, and a plurality of air outlet blocking beads are placed in the emulsion capsule bubble (2); the diameter of the air outlet blocking beads is larger than the diameter of the air outlet (21) and the diameter of the air outlet of the air inlet groove (5).

2. The experimental animal nose dropper according to claim 1, characterized in that The experimental animal nasal dropper is also provided with a limit screw (3) and a scale indicating rod (4); the outer tube wall of the injection tube body (11) is engraved with scales, and the front end of the limit screw (3) connected to the handle of the air inlet push rod (12) is provided with a scale indicating rod (4), and the front end of the scale indicating rod (4) indicates the scale on the outer tube wall; the scale indicating rod (4) matches the outer tube wall of the injection tube body (11).

3. The experimental animal nose dropper according to claim 1, characterized in that The push handle of the air inlet push rod (12) is opened in the middle, and the end of the liquid inlet push rod (13) passes through the opening of the push handle of the air inlet push rod (12) and is placed in the injection tube body (11).

4. The experimental animal nose dropper according to claim 1, characterized in that The experimental animals are rats and mice.

Images