Method and formula for in-situ construction of PRP gel tissue engineering compound for regeneration of different tissues

Abstract

The invention relates to the technical field of tissue and organ regeneration, and discloses a method and a formula for in-situ construction of an autologous PRP gel tissue engineering compound for regeneration of different tissues. The formula comprises thrombin freeze-dried powder, a calcium gluconate injection and a PRP liquid, and the method comprises the steps of under the sterile condition, injecting 2ml of the calcium gluconate injection into a 2000U thrombin freeze-dried powder ampoule bottle, uniformly shaking until the mixture is fully dissolved into a calcium gluconate / thrombin mixed solution, respectively connecting injectors with 0.5 ml of the prepared calcium gluconate / thrombin mixed solution and 4.5 ml of a PRP solution with trocar injection interfaces inside and outside a transplantation gun, and simultaneously and synchronously injecting the calcium gluconate / thrombin mixed solution and the autologous PRP, specifically, (1) injecting into an in-vitro saline water empty ampoule bottle, and standing to obtain PRP gel; and (2) conducting in-vivo damaged tissue and organ in-situ injection (the activity of thrombin is 100 U / ml, the concentration of calcium gluconate injection is 10 mg / ml, and the concentration of PRP liquid is 4.5 ml). According to the method, the PRP gel is rapidly prepared at a time by using trocar inside and outside the transplantation gun through a local injection method.

Description

technical field

[0001] The invention relates to the technical field of tissue and organ regeneration, in particular to a method and a formula for in situ construction of an autologous PRP gel tissue engineering compound for regeneration of different tissues. Background technique

[0002] The regeneration and repair of large tissue defects is extremely difficult, and the use of autologous tissue transplantation will cause bleeding, infection and new damage, and the source is limited. Large-area tissue defects caused by trauma, infection, tumors, burns, etc. are thorny problems in clinical treatment. Traditional autologous tissue or allogeneic tissue transplantation and biomaterial filling, due to various drawbacks, have unsatisfactory therapeutic effects and serious limit its clinical application.

[0003] At present, most tissue repair materials are scaffold materials loaded with cell growth factors. The selection of scaffold materials and growth factors is the focus of ti...

Examples

Embodiment 1

[0043] A formulation for the in situ construction of a PRP gel tissue engineering complex for regeneration of different tissues, comprising the following raw materials: freeze-dried thrombin powder, calcium gluconate injection, and PRP solution.

[0044] A method for in situ construction of a PRP gel tissue engineering complex for regeneration of different tissues, comprising the steps of:

[0045] The construction of PRP gel in vitro includes the following steps:

[0046] S1: Under sterile conditions, inject 2ml calcium gluconate injection (calcium gluconate 1g / 10ml) into 2000U thrombin freeze-dried powder ampoule, shake well until fully dissolved into calcium gluconate / thrombin mixture, use Draw 0.5ml of the solution with a 1ml syringe;

[0047] S2: Draw 4.5ml of PRP solution with a 10ml syringe, and the PRP is obtained by existing mechanical collection or manual collection.

[0048] S3: Connect the prepared syringes of 0.5ml of calcium gluconate / thrombin mixed solution an...

Embodiment 2

[0050] The difference between embodiment two and embodiment one is that a kind of method for the in situ construction of the PRP gel tissue engineering compound used for different tissue regeneration,

[0051] The construction of PRP gel in vivo includes the following steps:

[0052] S1: Under sterile conditions, inject 2ml of calcium gluconate injection (calcium gluconate 1g / 10ml) into 2000U thrombin freeze-dried powder ampoule, shake well to fully dissolve into calcium gluconate / thrombin mixture, use Draw 0.5ml of the solution with a 1ml syringe;

[0053] S2: Draw 4.5ml of PRP solution with a 10ml syringe

[0054] S3: Under the guidance of musculoskeletal ultrasound, connect the prepared syringes of 0.5ml of calcium gluconate / thrombin mixed solution and 4.5ml of PRP solution to the injection ports of the inner and outer catheters of the transplant gun, and simultaneously inject the calcium gluconate / thrombin The enzyme mixture and PRP are injected into the lesion, and PRP ...

Embodiment 3

[0057] The difference between Example 3 and Example 2 is that it is a method for in situ construction of PRP gel tissue engineering complexes for regeneration of different tissues. In this example, PRP gel is performed on lumbar disc degeneration in situ. Shaping, including the following steps:

[0058] S1: Under image guidance, the stem cell transplantation gun is used to puncture the trocar, and the needle tip is precisely positioned on the target site;

[0059] S2: Synchronously inject the platelet activator, namely 0.2ml calcium gluconate solution and 2ml PRP, into the target site in the body through two mutually isolated channels of the puncture trocar at the same time;

[0060] S3: The two liquids are fully mixed immediately before flowing out of the needle tip, forming a cell-gel composite scaffold at the target site that matches the shape and size of the lesion.

[0061] The experiment is as follows:

[0062] A. Connect the prepared 0.5ml NS syringe and 5ml PRP syrin...

technical field

[0001] The invention relates to the technical field of tissue and organ regeneration, in particular to a method and a formula for in situ construction of an autologous PRP gel tissue engineering compound for regeneration of different tissues. Background technique

[0002] The regeneration and repair of large tissue defects is extremely difficult, and the use of autologous tissue transplantation will cause bleeding, infection and new damage, and the source is limited. Large-area tissue defects caused by trauma, infection, tumors, burns, etc. are thorny problems in clinical treatment. Traditional autologous tissue or allogeneic tissue transplantation and biomaterial filling, due to various drawbacks, have unsatisfactory therapeutic effects and serious limit its clinical application.

[0003] At present, most tissue repair materials are scaffold materials loaded with cell growth factors. The selection of scaffold materials and growth factors is the focus of ti...

Examples

Embodiment 1

[0043] A formulation for the in situ construction of a PRP gel tissue engineering complex for regeneration of different tissues, comprising the following raw materials: freeze-dried thrombin powder, calcium gluconate injection, and PRP solution.

[0044] A method for in situ construction of a PRP gel tissue engineering complex for regeneration of different tissues, comprising the steps of:

[0045] The construction of PRP gel in vitro includes the following steps:

[0046] S1: Under sterile conditions, inject 2ml calcium gluconate injection (calcium gluconate 1g / 10ml) into 2000U thrombin freeze-dried powder ampoule, shake well until fully dissolved into calcium gluconate / thrombin mixture, use Draw 0.5ml of the solution with a 1ml syringe;

[0047] S2: Draw 4.5ml of PRP solution with a 10ml syringe, and the PRP is obtained by existing mechanical collection or manual collection.

[0048] S3: Connect the prepared syringes of 0.5ml of calcium gluconate / thrombin mixed solution an...

Embodiment 2

[0050] The difference between embodiment two and embodiment one is that a kind of method for the in situ construction of the PRP gel tissue engineering compound used for different tissue regeneration,

[0051] The construction of PRP gel in vivo includes the following steps:

[0052] S1: Under sterile conditions, inject 2ml of calcium gluconate injection (calcium gluconate 1g / 10ml) into 2000U thrombin freeze-dried powder ampoule, shake well to fully dissolve into calcium gluconate / thrombin mixture, use Draw 0.5ml of the solution with a 1ml syringe;

[0053] S2: Draw 4.5ml of PRP solution with a 10ml syringe

[0054] S3: Under the guidance of musculoskeletal ultrasound, connect the prepared syringes of 0.5ml of calcium gluconate / thrombin mixed solution and 4.5ml of PRP solution to the injection ports of the inner and outer catheters of the transplant gun, and simultaneously inject the calcium gluconate / thrombin The enzyme mixture and PRP are injected into the lesion, and PRP ...

Embodiment 3

[0057] The difference between Example 3 and Example 2 is that it is a method for in situ construction of PRP gel tissue engineering complexes for regeneration of different tissues. In this example, PRP gel is performed on lumbar disc degeneration in situ. Shaping, including the following steps:

[0058] S1: Under image guidance, the stem cell transplantation gun is used to puncture the trocar, and the needle tip is precisely positioned on the target site;

[0059] S2: Synchronously inject the platelet activator, namely 0.2ml calcium gluconate solution and 2ml PRP, into the target site in the body through two mutually isolated channels of the puncture trocar at the same time;

[0060] S3: The two liquids are fully mixed immediately before flowing out of the needle tip, forming a cell-gel composite scaffold at the target site that matches the shape and size of the lesion.

[0061] The experiment is as follows:

[0062] A. Connect the prepared 0.5ml NS syringe and 5ml PRP syrin...

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