Breeding method and application of blue-marked two-line method hybrid wheat system

Abstract

The invention discloses a breeding method and application of a blue-marked two-line method hybrid wheat system. The invention creates exogenous translocation chromosomes [T4AgL (a segment containing Ba)-4BL (a segment close to centromere). 4thS and T4AgL (a segment containing Ba)-4BL (a segment close to centromere). 4thS (a segment containing Rf)] and exogenous translocation telosomes [T4AgL (a segment containing Ba)-4thS (a segment containing Rf), T4thS (a segment containing Rf)-4AgL (a segment containing Ba), and T4thS (a segment containing Rf)-4AgL (a segment containing Ba)-4BL (a segment close to a centromere) which has complete restoring capacity for a recessivegenic male sterility ms1 and containing a blue grain gene and an exogenous restoring gene Rf expressing dosage effect and endosperm texture. The blue-marked two-line method hybrid wheat system is improved.

Description

technical field

[0001] The invention belongs to the field of crop genetics and breeding, and relates to a breeding method and application of a blue standard two-line hybrid wheat system. Background technique

[0002] Wheat is the most consumed food crop in the world and one of the main food crops in my country. With the decrease of arable land and the increase of population, it is imperative to increase the yield of wheat per unit area. The use of heterosis is an important way to increase the yield of crops. Rice, corn, sorghum and other crops have already been hybridized, and they have been widely used in production one after another, greatly increasing the yield. The utilization of heterosis in wheat has always been a difficult problem in the world, and no breakthrough has been made in production until now. The main reason is that wheat is polyploid, and it uses a large amount of seeds, and its heterosis is not as strong as that of rice, corn, sorghum and other crops. The...

Examples

Embodiment 1

[0183] Example 1. Creation of Translocation Chromosome T4AgL Fragment (Ba)-4BL·4thS(Rf)

[0184] 1. Obtain translocation chromosome T4AgL(Ba)-4BL·4BS(Rht3-ms1b).

[0185] (1) Recombine 4AgL(Ba) onto 4B to obtain translocation chromosome T4AgL(Ba)-4BL·4BS.

[0186] (2) The white-grained sterile plant of the 06L6109-3 dual-purpose line was crossed with the wheat with translocation chromosome T4AgL(Ba)-4BL·4BS (tall and male-fertile wheat grown from blue-grained seeds), and screened Dwarf, male-sterile plants grown from blue-seeded seeds.

[0187] White-grained sterile plant of 06L6109-3 dual-purpose line: it is a dwarf and male sterile plant grown from white-grained seeds obtained from selfing of 06L6109-3 dual-purpose line.

[0188] The 06L6109-3 dual-purpose line is a hybrid of ms1b-Rht3-containing wheat grown from white seeds with semi-dwarf and male sterility traits and the dual-purpose line obtained by the method in ZL200610042629.8. Rht3-ms1b blue-grain dual-purpose lin...

Embodiment 2

[0202] Example 2, Creation of Translocation Chromosome T4AgL Fragment (Ba)-4BL·4thS Fragment (Rf)

[0203] 1. The establishment of the endosome addition line containing the Rf gene and the endosome addition line containing the Ba gene.

[0204] From the translocation line 14T35 obtained in Example 1, the 15L4167 dual-purpose line was bred through generation selection (except for the poor stability of the additional chromosome, the rest of the agronomic traits have been stabilized). The 15L4167 dual-purpose line is an addition line (dual-purpose line) containing the translocation chromosome T4AgL fragment (Ba)-4BL·4thS(Rf). The karyotype of 15L4167 dual-purpose line is: 21Ⅱ(msms)+Ⅰ T4AgL片段(Ba)-4BL·4thS(Rf) .

[0205] The 15L4167 dual-purpose line was selfed, and then the male sterile plants grown from blue-grained seeds were screened, that is, the plants with the translocated terminal body T4AgL fragment (Ba)-4BL·. This plant is crossed with dark blue grain fertile plants (1...

Embodiment 3

[0215] Example 3, Translocation Endosome T4AgL Fragment (Ba)-4thS Fragment (Rf), Translocation Endosome T4thS Fragment (Rf)-4AgL Fragment (Ba) Or Translocation Endosome T4thS Fragment (Rf)-4AgL Fragment Creation of (Ba)-4BL·

[0216] 1. Creation of Translocation Endosome T4AgL Fragment (Ba)-4thS Fragment (Rf) and Translocation Endosome T4thS Fragment (Rf)-4AgL Fragment (Ba)

[0217] 1. Obtain the additional line of double end body.

[0218] (1) The karyotype of the dual-purpose line cultivated in the invention patent (ZL200610042629.8) is: 21Ⅱ(msms)+Ⅰ T4thS·4AgL ; That is, the addition line wheat (16L6386 dual-purpose line) containing the translocation chromosome T4thS·4AgL.

[0219] (2) The 16L6386 dual-purpose line was selfed, the blue-grained seeds were collected, planted and cultivated into plants, and the male sterile plants were selected, and the plants with the terminal body 4AgL(Ba)· were screened by microscopy.

[0220] (3) The 16L6386 dual-purpose line was selfed,...

technical field

[0001] The invention belongs to the field of crop genetics and breeding, and relates to a breeding method and application of a blue standard two-line hybrid wheat system. Background technique

[0002] Wheat is the most consumed food crop in the world and one of the main food crops in my country. With the decrease of arable land and the increase of population, it is imperative to increase the yield of wheat per unit area. The use of heterosis is an important way to increase the yield of crops. Rice, corn, sorghum and other crops have already been hybridized, and they have been widely used in production one after another, greatly increasing the yield. The utilization of heterosis in wheat has always been a difficult problem in the world, and no breakthrough has been made in production until now. The main reason is that wheat is polyploid, and it uses a large amount of seeds, and its heterosis is not as strong as that of rice, corn, sorghum and other crops. The...

Examples

Embodiment 1

[0183] Example 1. Creation of Translocation Chromosome T4AgL Fragment (Ba)-4BL·4thS(Rf)

[0184] 1. Obtain translocation chromosome T4AgL(Ba)-4BL·4BS(Rht3-ms1b).

[0185] (1) Recombine 4AgL(Ba) onto 4B to obtain translocation chromosome T4AgL(Ba)-4BL·4BS.

[0186] (2) The white-grained sterile plant of the 06L6109-3 dual-purpose line was crossed with the wheat with translocation chromosome T4AgL(Ba)-4BL·4BS (tall and male-fertile wheat grown from blue-grained seeds), and screened Dwarf, male-sterile plants grown from blue-seeded seeds.

[0187] White-grained sterile plant of 06L6109-3 dual-purpose line: it is a dwarf and male sterile plant grown from white-grained seeds obtained from selfing of 06L6109-3 dual-purpose line.

[0188] The 06L6109-3 dual-purpose line is a hybrid of ms1b-Rht3-containing wheat grown from white seeds with semi-dwarf and male sterility traits and the dual-purpose line obtained by the method in ZL200610042629.8. Rht3-ms1b blue-grain dual-purpose lin...

Embodiment 2

[0202] Example 2, Creation of Translocation Chromosome T4AgL Fragment (Ba)-4BL·4thS Fragment (Rf)

[0203] 1. The establishment of the endosome addition line containing the Rf gene and the endosome addition line containing the Ba gene.

[0204] From the translocation line 14T35 obtained in Example 1, the 15L4167 dual-purpose line was bred through generation selection (except for the poor stability of the additional chromosome, the rest of the agronomic traits have been stabilized). The 15L4167 dual-purpose line is an addition line (dual-purpose line) containing the translocation chromosome T4AgL fragment (Ba)-4BL·4thS(Rf). The karyotype of 15L4167 dual-purpose line is: 21Ⅱ(msms)+Ⅰ T4AgL片段(Ba)-4BL·4thS(Rf) .

[0205] The 15L4167 dual-purpose line was selfed, and then the male sterile plants grown from blue-grained seeds were screened, that is, the plants with the translocated terminal body T4AgL fragment (Ba)-4BL·. This plant is crossed with dark blue grain fertile plants (1...

Embodiment 3

[0215] Example 3, Translocation Endosome T4AgL Fragment (Ba)-4thS Fragment (Rf), Translocation Endosome T4thS Fragment (Rf)-4AgL Fragment (Ba) Or Translocation Endosome T4thS Fragment (Rf)-4AgL Fragment Creation of (Ba)-4BL·

[0216] 1. Creation of Translocation Endosome T4AgL Fragment (Ba)-4thS Fragment (Rf) and Translocation Endosome T4thS Fragment (Rf)-4AgL Fragment (Ba)

[0217] 1. Obtain the additional line of double end body.

[0218] (1) The karyotype of the dual-purpose line cultivated in the invention patent (ZL200610042629.8) is: 21Ⅱ(msms)+Ⅰ T4thS·4AgL ; That is, the addition line wheat (16L6386 dual-purpose line) containing the translocation chromosome T4thS·4AgL.

[0219] (2) The 16L6386 dual-purpose line was selfed, the blue-grained seeds were collected, planted and cultivated into plants, and the male sterile plants were selected, and the plants with the terminal body 4AgL(Ba)· were screened by microscopy.

[0220] (3) The 16L6386 dual-purpose line was selfed,...

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