A real-time blood lipid detection system based on magnetic microdimer robot

Abstract

The present invention relates to a blood lipid measurement system, more specifically, a blood lipid instant detection system based on a magnetic micro-dimer robot. The external magnetic field generating device controls the movement of the micro-dimer robot, collects the movement behavior information of the micro-dimer robot through the movement information acquisition unit and transmits it to the PC module, the PC module analyzes the movement posture and movement speed of the micro-dimer robot, and measures the concentration of blood lipids in the blood, that is, the concentration of cholesterol and triglycerides by calculating the peak speed of the micro-dimer robot and the change trend of the precession angle during the movement. The magnetic micro-dimer robot is controlled to move in blood with different blood lipid concentrations through an external magnetic field, and its movement behavior is collected in time, the relationship between the movement speed and movement posture and the blood lipid inverse concentration and magnetic field parameters is analyzed, and the parameters with the strongest functional relationship are found as the measurement parameters used in blood lipid detection to achieve timely detection of the blood lipid concentration of the blood sample to be tested.

Description

Technical Field

[0001] The invention relates to a blood lipid measurement system, more specifically to a blood lipid instant detection system based on a magnetic micro-dimer robot. Background Art

[0002] Blood lipids are the general term for neutral fats and lipids in the blood. They are an important indicator for measuring the health of the human body. Changes in blood lipids can basically reflect the state of lipid metabolism in the body. Diseases such as atherosclerosis, coronary heart disease, cerebral thrombosis, hypertension, and hyperlipidemia can be prevented in advance by testing blood lipid content. Nowadays, the commonly used blood lipid detection method is to extract the patient's plasma or serum and complete the blood lipid determination on a fully automatic biochemical analyzer. This method has high detection accuracy, but the instrument operation is complicated, the reaction time is long, and the sample blood collection volume is large, which brings great pain to the patient and is not conducive to bedside instant detection. Blood lipid testing mainly detects cholesterol and triglycerides, so it is very important to design a blood lipid detection method with a high degree of automation and fast response time. Summary of the invention

[0003] In order to overcome the shortcomings of the prior art, the present invention provides an instant blood lipid detection system based on a magnetic micro-dimer robot, which controls the magnetic micro-dimer robot to move in blood with different blood lipid concentrations through an external magnetic field, and timely collects its movement behavior, analyzes the relationship between the movement speed and movement posture and the blood lipid inverse concentration and magnetic field parameters, and finds the parameters with the strongest functional relationship as the measurement parameters used in blood lipid detection to achieve timely detection of the blood lipid concentration of the blood sample to be tested.

[0004] The technical solution adopted by the present invention to solve its technical problem is:

[0005] A blood lipid instant detection system based on a magnetic micro-dimer robot comprises an external magnetic field generating device, a motion information acquisition unit and a PC module. The PC module controls the external magnetic field generating device to generate a magnetic field, thereby controlling the movement of the micro-dimer robot. The motion information acquisition unit collects the motion behavior information of the micro-dimer robot and transmits it to the PC module. The PC module analyzes the motion posture and motion speed of the micro-dimer robot, and measures the blood lipid concentration, that is, the concentration of cholesterol and triglycerides, in the blood by calculating the peak speed of the micro-dimer robot and the change trend of the dynamic angle during the movement.

[0006] As a further optimization of the present invention, the external magnetic field generating device generates rotating magnetic fields of different intensities and frequencies to control the movement of the micro-dimer robot.

[0007] As a further optimization of the present invention, the PC module sends a command signal to an external magnetic field generating device to generate a magnetic field to drive the micro-dimer robot to move in the blood.

[0008] As a further optimization of the present invention, the external magnetic field generating device includes three groups of orthogonally distributed Helmholtz coils, and the three groups of Helmholtz coils are respectively arranged in three axial directions of spatial coordinates x, y and z.

[0009] As a further optimization of the present invention, an aluminum plate with a hollow center is fixed in the middle of the three groups of Helmholtz coils as an observation platform, and the centers of the three groups of Helmholtz coils overlap with the center of the observation platform.

[0010] As a further optimization of the present invention, sinusoidal currents are applied in the x-axis and z-axis directions to control three groups of Helmholtz coils to generate a rotating magnetic field in the xoz plane, thereby driving the micro-dimer robot to move.

[0011] As a further optimization of the present invention, the magnetic micro-dimer robot is a micro-dimer formed by self-assembly of magnetic Janus microspheres.

[0012] The peak speed is the maximum movement speed that the micro-nano robot can reach under a fixed magnetic field strength as the magnetic field frequency changes, and has a linear function relationship with a large slope to the cholesterol concentration in the blood.

[0013] The precession angle is the angle between the long axis of the micro-nano robot and the direction of motion. At the mid-range frequency, the precession angle changes significantly with the change of triglyceride concentration.

[0014] As a further optimization of the present invention, based on the measurement of the movement speed, the peak speed of the micro-dimer robot is analyzed to calculate the concentration of cholesterol in the blood. The measurable cholesterol concentration range reaches 1000 mg / dL.

[0015] As a further optimization of the present invention, based on the analysis of the change process of the precession angle, the precession angle of the micro-dimer robot at the mid-segment magnetic field frequency of 9, 10 and 11 Hz is measured to calculate the concentration of triglycerides in the blood. The measurable triglyceride concentration range reaches 3000 mg / mL.

[0016] The beneficial effects of the instant blood lipid detection system based on the magnetic micro-dimer robot of the present invention are:

[0017] 1. This detection system only requires 0.1 ml of blood sample each time;

[0018] 2. The detection system has a high degree of automation, which can greatly reduce the workload of doctors. It is also easy to operate and convenient for doctors to use for bedside detection.

[0019] 3. Compared with traditional biochemical analyzers, this detection system requires significantly less time, making it easier for doctors to take appropriate medical measures in a timely manner;

[0020] 4. The blood lipid concentration can be accurately measured by measuring the peak speed and precession angle change trend of the magnetic micro-dimer robot during movement under the action of the mid-frequency magnetic field. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] The present invention is further described in detail below with reference to the accompanying drawings and specific implementation methods.

[0022] Figure 1 Provide a blood lipid test flow chart for the test system;

[0023] Figure 2 This is the function graph of the maximum speed of the micro-dimer robot and the cholesterol concentration;

[0024] Figure 3 This is the function image of the relationship between the movement speed of the micro-nano robot and the frequency at different triglyceride concentrations;

[0025] Figure 4 This is the function graph of the relationship between the step angle and triglyceride concentration at the mid-frequency. DETAILED DESCRIPTION

[0026] A blood lipid instant detection system based on a magnetic micro-dimer robot comprises an external magnetic field generating device, a motion information acquisition unit and a PC module. The external magnetic field generating device controls the movement of the micro-dimer robot. The motion information acquisition unit collects the motion behavior information of the micro-dimer robot and transmits it to the PC module. The PC module analyzes the motion posture and motion speed of the micro-dimer robot, and measures the concentrations of cholesterol and triglycerides in the blood by calculating the peak speed of the micro-dimer robot and the change trend of the precession angle during the movement at the mid-frequency.

[0027] The PC module sends a command signal to the external magnetic field generating device to generate a magnetic field to drive the micro-dimer robot to move in the blood. The command signal is a command sent by the computer through a program to control the data board to generate a sinusoidal voltage, which is amplified by the power amplifier and input into the coil group, and the driving coil generates a magnetic field to drive the dimer micro-nano robot to move. The parameters of the magnetic field can be controlled by controlling the amplitude, phase and frequency of the output voltage.

[0028] Figure 1The figure shows the process of blood lipid detection. The blood sample is collected and dripped on a glass slide. The glass slide is placed in a magnetic field driving device and a magnetic micro-dimer robot is added. The external magnetic field generating device generates magnetic fields of different strengths and frequencies to drive the micro-dimer robot to move. The motion information acquisition unit collects the motion behavior information of the micro-dimer robot under the action of external magnetic fields of different strengths and frequencies in real time and returns it to the PC calculation module to analyze the motion posture of the micro-dimer robot, calculate the peak speed of the micro-dimer robot and the change trend of the precession angle during the movement, and thus measure the concentration of cholesterol and triglycerides in the blood.

[0029] like Figure 2 As shown, through a large number of experimental studies, it was found that the peak speed of the micro-dimer robot and the cholesterol concentration are a linear function with a large slope. The cholesterol concentration in the blood can be accurately determined by measuring the peak speed of the micro-dimer robot.

[0030] like Figure 3 The magnetic micro-dimer robot shown in the figure has an insignificant change in precession angle with frequency at high and low frequencies, but has a significant change in precession angle with frequency at mid-range frequencies, and the precession angle varies with different triglyceride concentrations.

[0031] like Figure 4 As shown in the figure, the experiment concluded that the precession angle of the magnetic micro-dimer machine at the mid-frequency has a good linear relationship with the triglyceride concentration. The predetermined magnetic field is used to drive the dimer micro-nano robot to move, and the image processing software can be used to obtain the precession angle of the dimer micro-nano robot at the mid-frequency. Then, combined with the experimental data, the triglyceride concentration in the blood can be determined.

[0032] Among them, through a large number of studies, it was found that the step angle of the micro-dimer robot under the action of a magnetic field of 9, 10, and 11 Hz decreases linearly with the decrease of triglyceride concentration. The triglyceride concentration in the blood can be determined by measuring the step angle of the micro-dimer robot under the action of a magnetic field of 9, 10, and 11 Hz.

[0033] Among them, the external magnetic field generating device includes three groups of orthogonally distributed Helmholtz coils, which are arranged in the three axial directions of the spatial coordinates x, y and z, respectively, to form a three-dimensional Helmholtz coil, which can generate various types of magnetic fields in any plane. An aluminum plate with a center left empty is fixed in the middle of the three groups of Helmholtz coils as an observation platform, and the centers of the three groups of Helmholtz coils overlap with the center of the observation platform. A glass slide is placed in the center of the observation platform, and the sample is dripped on the glass slide, so that a uniform rotating magnetic field can be generated in the sample area to ensure the accuracy and stability of the test. The intensity and frequency of the magnetic field generated by the three groups of coils are regulated by adjusting the magnitude and frequency of the current. When a sinusoidal current is applied to the x-axis and z-axis, a rotating magnetic field in the xoz plane is generated to drive the micro-dimer robot to move to feedback information.

[0034] Among them, the motion information acquisition unit transmits the collected motion behavior back to the PC control module based on the biological living multimodal imaging technology for further analysis and calculation.

[0035] Among them, the PC module mainly includes image analysis software and motion command output software. The motion command output software is used to issue instructions to the external magnetic field generating device to generate a magnetic field with an intensity of 5mT and different frequencies. Using the image analysis software, the motion images of the micro-dimer robot under different magnetic fields are collected, and the collected images are analyzed using the difference method to analyze the motion posture of the micro-dimer robot, and the peak speed of the micro-dimer robot and the precession angle at the mid-range frequency of 9, 10, and 11Hz are calculated. Combined with the calibration data, the concentration of blood lipids in the blood is determined. This method can measure the cholesterol and triglyceride concentrations in the range of 1000mg / dL and 3000mg / mL, respectively.

[0036] The peak speed is the maximum movement speed that the micro-nano robot can reach under a fixed magnetic field strength as the magnetic field frequency changes, and has a linear function relationship with a large slope to the cholesterol concentration in the blood.

[0037] The precession angle is the angle between the long axis of the micro-nano robot and the direction of motion. At the mid-range frequency, the precession angle changes significantly with the change of triglyceride concentration.

[0038] The magnetic micro-dimer robot is a micro-dimer formed by self-assembly of magnetic Janus microspheres.

[0039] The preparation process of the magnetic Janus microspheres comprises the following steps:

[0040] Step 1: evenly spread a single layer of silica microspheres on a glass slide;

[0041] Step 2: using an ion sputtering device to plate a 100 nm thick nickel layer on the silicon dioxide microspheres;

[0042] Step 3: Using a water bath sonicator, the microparticles were released from the matrix, washed three times, and then dispersed in deionized water;

[0043] Step 4: Place the microparticles in a 2 mg / ml PEI solution, shake for 20 minutes, and then wash with DIW three times; then place the microparticles in a 2 mg / ml PSS solution, wash with DIW three times, and then place in a 2 mg / ml PAH solution. This process requires six cycles;

[0044] Step 5: The whole blood was centrifuged at 4°C, and washed three times with 4°C 1×PBS ice water. The washed red blood cells were placed in an ice bath in a 0.25×PBS solution for 20 minutes, centrifuged, and the pink substance at the bottom was collected. Subsequently, an extruder was used to pass the red blood cell membrane through 5μm, 3μm, 1μm, 800nm, 600m, 400nm, 200nm and 100nm polycarbonate porous membranes respectively.

[0045] Step 6: Mix the treated microspheres with the red blood cell membranes, sonicate for 2 hours, and continue to oscillate for 24 hours;

[0046] Step seven: applying an external magnetic field to allow the microspheres to self-assemble into microdimers.

[0047] Using this process to coat the red blood cell membrane on the surface of the micro-nano robot can effectively prevent the micro-nano robot from agglomerating in the blood. Step 4: Apply an external magnetic field to make the microspheres self-assemble to form micro dimers.

Claims

1. A blood lipid instant detection system based on a magnetic micro-dimer robot, comprising an external magnetic field generating device, a motion information acquisition unit and a PC module, characterized in that: The external magnetic field generating device generates a rotating uniform magnetic field to control the movement of the micro-dimer robot, and the movement behavior information of the micro-dimer robot is collected by the movement information collection unit and transmitted to the PC module. The PC module analyzes the movement posture and movement speed of the micro-dimer robot, and measures the concentration of cholesterol and triglyceride in the blood by calculating the peak speed of the micro-dimer robot and the change trend of the precession angle during the movement under the action of the mid-frequency magnetic field; The external magnetic field generating device comprises three groups of orthogonally distributed Helmholtz coils, wherein the three groups of Helmholtz coils are arranged in three axial directions of spatial coordinates x, y and z respectively; The magnetic micro-dimer robot is a micro-dimer formed by self-assembly of magnetic Janus microspheres; The peak speed is the maximum movement speed that the micro-nano robot can reach under a fixed magnetic field strength as the magnetic field frequency changes, and has a linear function relationship with the cholesterol concentration in the blood; The precession angle is the angle between the long axis of the micro-nano robot and the moving direction.

2. The blood lipid instant detection system based on the magnetic micro-dimer robot according to claim 1 is characterized in that: The external magnetic field generating device generates rotating magnetic fields of different intensities and frequencies to control the movement of the micro-dimer robot.

3. The instant blood lipid detection system based on a magnetic micro-dimer robot according to claim 1 is characterized in that: The PC module sends a command signal to an external magnetic field generating device to generate a rotating magnetic field of the same magnitude and increasing frequency to drive the micro-dimer robot to move in the blood.

4. The instant blood lipid detection system based on a magnetic micro-dimer robot according to claim 1 is characterized in that: An aluminum plate with a hollow center is fixed in the middle of the three groups of Helmholtz coils as an observation platform, and the centers of the three groups of Helmholtz coils overlap with the center of the observation platform.

5. The instant blood lipid detection system based on the magnetic micro-dimer robot according to claim 4 is characterized in that: By applying sinusoidal current in the x-axis and z-axis directions, three groups of Helmholtz coils are controlled to generate a rotating magnetic field in the xoz plane, thereby driving the micro-dimer robot to move.

6. The instant blood lipid detection system based on a magnetic micro-dimer robot according to claim 1, characterized in that: The preparation process of the magnetic Janus microspheres comprises the following steps: Step 1: evenly spread a single layer of silica microspheres on a glass slide; Step 2: using an ion sputtering device to plate a 100 nm thick nickel layer on the silicon dioxide microspheres; Step 3: Using a water bath sonicator, the microparticles were released from the matrix, washed three times, and then dispersed in deionized water; Step 4: Place the microparticles in a 2 mg / ml PEI solution, shake for 20 minutes, and then wash with DIW three times; then place the microparticles in a 2 mg / ml PSS solution, wash with DIW three times, and then place in a 2 mg / ml PAH solution. This process requires six cycles; Step 5: The whole blood was centrifuged at 4°C, and washed three times with 4°C 1×PBS ice water. The washed red blood cells were placed in an ice bath in a 0.25×PBS solution for 20 minutes, centrifuged, and the pink substance at the bottom was collected. Subsequently, an extruder was used to pass the red blood cell membrane through 5μm, 3μm, 1μm, 800nm, 600m, 400nm, 200nm and 100nm polycarbonate porous membranes respectively. Step 6: Mix the treated microspheres with the red blood cell membranes, sonicate for 2 hours, and continue to oscillate for 24 hours; Step seven: applying an external magnetic field to allow the microspheres to self-assemble into microdimers.

Images