Mass spectrometry data acquisition device and method based on multi-channel technology

Abstract

The present invention provides a mass spectrometry data acquisition device and method based on multi-channel technology, the mass spectrometry data acquisition device based on multi-channel technology includes a repeller and a first detector; further includes: the first detector and the second detector are arranged in sequence, and the tolerance to ion intensity is gradually improved; a calculation unit is used to obtain a first mapping relationship between the output ion intensity of the first detector and the second detector according to the output of ions of the same intensity on the first detector and the second detector respectively; and the output ion intensity of the second detector is converted according to the first mapping relationship; a memory is used to store the mapping relationship; a control unit is used to adjust the repeller voltage so that the incident ions are deflected to the selected detector; an output unit is used to obtain the ion intensity and flight time according to the conversion results output by the first detector and the calculation unit. The present invention has the advantages of long service life.

Description

Technical Field

[0001] The present invention relates to mass spectrometry analysis, and in particular to a mass spectrometry data acquisition device and method based on multi-channel technology. Background Art

[0002] Time of Flight Mass Spectrometer (TOFMS) is a commonly used mass spectrometer. The technical principle is: give ions of different mass-to-charge ratios the same initial kinetic energy, so that the ions reach the detector through the same flight path, and distinguish ions of different mass-to-charge ratios according to the arrival time, so as to complete the qualitative and quantitative analysis of the substance. In the time-of-flight mass spectrometer, a high-gain microchannel plate is generally used as a detector. However, the characteristics of the full spectrum analysis of the time-of-flight result in that if the microchannel plate is subjected to the impact of high-intensity ion flow for a long time during use, it is easy to cause performance degradation and greatly shorten the service life.

[0003] To solve the above problems, the following technical solutions are usually adopted:

[0004] 1. Before the ions enter the time-of-flight mass spectrometer, the high-intensity ions are filtered using quadrupole transmission;

[0005] 2. Apply pulses to the grid at the front of the detector to remove unwanted ions before they reach the microchannel plate

[0006] 3. By applying a pulse voltage to the outer sector shell of the sector energy analyzer, some ions are screened out or selected for mass spectrometry detection to eliminate the influence of interfering ions.

[0007] In the above-mentioned solutions, different methods are used to filter high-intensity ions to reduce damage to the microchannel plate detector. However, the disadvantage is that these methods will cause the loss of filtered ion information and fail to obtain full spectrum data. Summary of the invention

[0008] In order to solve the deficiencies in the above-mentioned prior art solutions, the present invention provides a mass spectrometry data acquisition device based on multi-channel technology.

[0009] The objective of the present invention is achieved through the following technical solutions:

[0010] The mass spectrometry data acquisition device based on multi-channel technology comprises a repeller and a first detector; the mass spectrometry data acquisition device based on multi-channel technology further comprises:

[0011] A second detector, wherein the first detector and the second detector are arranged in sequence and have gradually increasing tolerance to ion strength;

[0012] A calculation unit, the calculation unit is used to obtain a first mapping relationship for conversion between the output ion intensities of the first detector and the second detector according to the outputs of ions of the same intensity on the first detector and the second detector respectively; and convert the output ion intensity of the second detector according to the first mapping relationship;

[0013] A memory, wherein the memory is used to store the mapping relationship;

[0014] a control unit, the control unit being used to adjust the repeller voltage so that incident ions are deflected to a selected detector;

[0015] An output unit is used to obtain ion intensity and flight time according to the conversion results output by the first detector and the calculation unit.

[0016] The object of the present invention is also to provide a mass spectrometry data acquisition method based on multi-channel technology, and the object of the invention is achieved through the following technical solutions:

[0017] A mass spectrometry data acquisition method based on multi-channel technology, the mass spectrometry data acquisition method based on multi-channel technology comprising the following steps:

[0018] (A1) Ions enter the first detector, which outputs ion intensity information and flight time;

[0019] (A2) dividing the intensity intervals according to the intensity distribution in the ion intensity information, each intensity interval corresponds to a detector, and the greater the ion intensity, the higher the tolerance of the corresponding detector to the ion intensity, thereby obtaining the corresponding relationship between the ion, the detector, and the flight time;

[0020] (A3) ion incidence, repelling the ions to a detector corresponding to the ions according to the corresponding relationship, and obtaining an output ion intensity of the detector;

[0021] (A4) obtaining a mapping relationship for conversion between detector outputs according to ion intensities of ions with the same intensity outputted by different detectors, and saving the mapping relationship;

[0022] (A5) ion incidence, repelling the ions to a detector corresponding to the ions according to the corresponding relationship, and obtaining an output ion intensity of the detector;

[0023] (A6) converting the ion intensity output by each detector according to the mapping relationship;

[0024] (A7) Output ion intensity and flight time.

[0025] Compared with the prior art, the present invention has the following beneficial effects:

[0026] 1. Long service life;

[0027] The ions are separated twice by using the different flight times, so that ions of different intensities enter the appropriate detection channel (each detector has different tolerance to ion intensity) for detection, thus avoiding the damage of the MCP detector by high-intensity ions, extending its service life, and obtaining full spectrum data at the same time;

[0028] 2. Obtain accurate data;

[0029] A two-stage pre-scan + formal scan method is used to solve the problem of different responses of different detectors (detection channels) and improve the accuracy of the obtained data;

[0030] 3. Comprehensive data;

[0031] The multi-channel detection mode (multiple detectors) is adopted to avoid the problem that the MCP detector cannot detect supersaturated ions and improve the dynamic range of ion detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] The disclosure of the present invention will become easier to understand with reference to the accompanying drawings. It is easy for those skilled in the art to understand that these drawings are only used to illustrate the technical solution of the present invention and are not intended to limit the scope of protection of the present invention. In the drawings:

[0033] Figure 1 It is a flow chart of a mass spectrometry data acquisition method based on multi-channel technology according to an embodiment of the present invention. DETAILED DESCRIPTION

[0034] Figure 1 The following description describes the optional embodiments of the present invention to teach those skilled in the art how to implement and reproduce the present invention. In order to explain the technical solution of the present invention, some conventional aspects have been simplified or omitted. Those skilled in the art will understand that variations or replacements derived from these embodiments will be within the scope of the present invention. Those skilled in the art will understand that the following features can be combined in various ways to form multiple variations of the present invention. Thus, the present invention is not limited to the following optional embodiments, but is only limited by the claims and their equivalents.

[0035] Embodiment 1:

[0036] The mass spectrometry data acquisition device based on multi-channel technology according to the embodiment of the present invention comprises:

[0037] a repeller and a first detector;

[0038] A second detector, wherein the first detector and the second detector are arranged in sequence, and the tolerance to ion intensity gradually increases, that is, the tolerance to ion intensity of the second detector is higher than that of the first detector;

[0039] A calculation unit, the calculation unit is used to obtain a first mapping relationship for conversion between the output ion intensities of the first detector and the second detector according to the outputs of ions of the same intensity on the first detector and the second detector respectively; and convert the output ion intensity of the second detector according to the first mapping relationship;

[0040] A memory, wherein the memory is used to store the mapping relationship;

[0041] a control unit, the control unit being used to adjust the repeller voltage so that incident ions are deflected to a selected detector;

[0042] An output unit is used to obtain ion intensity and flight time according to the conversion results output by the first detector and the calculation unit.

[0043] In order to adapt to the reception of ions of different intensities, the mass spectrometry data acquisition device further includes:

[0044] A third detector, wherein the first detector, the second detector and the third detector are arranged in sequence, and the tolerance to ion strength is gradually increased;

[0045] The calculation unit is used to obtain a second mapping relationship between the output ion intensities of the first detector and the third detector according to the output of ions of the same intensity on the first detector, the second detector and the third detector, and send it to the memory for storage; and convert the output ion intensity of the third detector according to the second mapping relationship;

[0046] The output unit is used to obtain the ion intensity according to the conversion results output by the first detector and the calculation unit.

[0047] Mass spectrometry data acquisition methods based on multi-channel technology, such as Figure 1 As shown, the mass spectrometry data acquisition method based on multi-channel technology includes the following steps:

[0048] (A1) Ions enter the first detector, which outputs ion intensity information and flight time;

[0049] (A2) dividing the intensity intervals according to the intensity distribution in the ion intensity information, each intensity interval corresponds to a detector, and the greater the ion intensity, the higher the tolerance of the corresponding detector to the ion intensity, thereby obtaining the corresponding relationship between the ion, the detector, and the flight time;

[0050] (A3) ion incidence, repelling the ions to a detector corresponding to the ions according to the corresponding relationship, and obtaining an output ion intensity of the detector;

[0051] (A4) obtaining a mapping relationship for conversion between detector outputs according to ion intensities of ions with the same intensity outputted by different detectors, and saving the mapping relationship;

[0052] (A5) ion incidence, repelling the ions to a detector corresponding to the ions according to the corresponding relationship, and obtaining an output ion intensity of the detector;

[0053] (A6) converting the ion intensity output by each detector according to the mapping relationship;

[0054] (A7) Output ion intensity and flight time.

[0055] In order to reduce the complexity of the structure and the difficulty of operation, further, along the arrangement direction of each detector, the tolerance of the detector to the ion strength is gradually improved.

[0056] Embodiment 2:

[0057] An application example of the mass spectrometry data acquisition device and method based on multi-channel technology according to Example 1 of the present invention.

[0058] In this application example, the first detector is a microchannel plate detector, the second detector is a Faraday cup, and the third detector is a charge receiving plate, and the individual detectors are arranged in sequence.

[0059] The mass spectrometry data acquisition method based on multi-channel technology, that is, the working method of the mass spectrometry data acquisition device of this embodiment, is as follows: Figure 1 As shown, the mass spectrometry data acquisition method based on multi-channel technology includes the following steps:

[0060] (A1) Pre-scan 1, ions enter the first detector, and output ion intensity information and flight time;

[0061] (A2) according to the intensity distribution in the ion intensity information, the intensity intervals are divided into: the intensity below 10,000 is a low intensity interval, 10,000 to 20,000 is a medium intensity interval, and the intensity above 20,000 is a high intensity interval; each intensity interval corresponds to a detector, the greater the ion intensity, the higher the tolerance of the corresponding detector to the ion intensity, the low intensity interval corresponds to a microchannel plate detector, the medium intensity interval corresponds to a Faraday cup, and the high intensity interval corresponds to a charge receiving plate, thereby obtaining a corresponding relationship between the ion and the detector and the flight time;

[0062] (A3) Pre-scan 2, ion incidence, according to the corresponding relationship, the ions are repelled to the detector corresponding to the ions, and the output ion intensity of the detector is obtained;

[0063] (A4) obtaining a mapping relationship for conversion between detector outputs, usually a linear relationship, based on the ion intensities of ions with the same intensity outputted by different detectors, and saving the mapping relationship;

[0064] (A5) Formal scanning, ion incidence, and according to the corresponding relationship, the ions are repelled to the detector corresponding to the ions, and the output ion intensity of the detector is obtained;

[0065] (A6) converting the ion intensity output by each detector according to the mapping relationship;

[0066] (A7) Output ion intensity and flight time.

Claims

1. A mass spectrometry data acquisition method based on a multi-channel technology according to a mass spectrometry data acquisition device based on a multi-channel technology, wherein the mass spectrometry data acquisition device comprises a repeller and a first detector; The mass spectrometry data acquisition device also includes: A second detector, wherein the first detector and the second detector are arranged in sequence and have gradually increasing tolerance to ion strength; A calculation unit, the calculation unit is used to obtain a first mapping relationship for conversion between the output ion intensities of the first detector and the second detector according to the outputs of ions of the same intensity on the first detector and the second detector respectively; and convert the output ion intensity of the second detector according to the first mapping relationship; A memory, wherein the memory is used to store the first mapping relationship; A control unit, the control unit is used to adjust the repeller voltage so that the incident ions are deflected to the selected detector; the first detector and the second detector correspond to different ion intensity intervals, respectively, and the ion intensity in the ion intensity interval corresponding to the second detector is greater than the ion intensity in the ion intensity interval corresponding to the first detector; an output unit, the output unit being used to obtain ion intensity and flight time according to the conversion results output by the first detector and the calculation unit; The mass spectrometry data acquisition method based on multi-channel technology comprises the following steps: (A1) Pre-scan 1, ions enter the first detector, and output ion intensity information and flight time; (A2) according to the intensity distribution in the ion intensity information, intensity intervals are divided, each intensity interval corresponds to a detector, the greater the ion intensity, the higher the tolerance of the corresponding detector to the ion intensity, thereby obtaining a corresponding relationship between the ion, the detector, and the flight time; the first detector and the second detector correspond to different ion intensity intervals, respectively, and the ion intensity in the ion intensity interval corresponding to the second detector is greater than the ion intensity in the ion intensity interval corresponding to the first detector; (A3) Pre-scan 2, ion incidence, according to the corresponding relationship, the ions are repelled to the detector corresponding to the ions, and the output ion intensity of the detector is obtained; (A4) obtaining a mapping relationship for conversion between detector outputs based on the ion intensities of ions with the same intensity outputted by different detectors, and saving the mapping relationship; (A5) ion incidence, repelling the ions to a detector corresponding to the ions according to the corresponding relationship, and obtaining an output ion intensity of the detector; (A6) converting the ion intensity output by each detector according to the mapping relationship; (A7) Output ion intensity and flight time.

2. The mass spectrometry data acquisition method based on multi-channel technology according to claim 1, characterized in that: Along the arrangement direction of each detector, the tolerance of the detector to ion intensity gradually increases.

3. The mass spectrometry data acquisition method based on multi-channel technology according to claim 1, characterized in that: The mass spectrometry data acquisition device also includes: A third detector, wherein the first detector, the second detector and the third detector are arranged in sequence, and the tolerance to ion strength is gradually increased; The calculation unit is used to obtain a second mapping relationship between the output ion intensities of the first detector and the third detector according to the output of ions of the same intensity on the first detector, the second detector and the third detector, and send it to the memory for storage; and convert the output ion intensity of the third detector according to the second mapping relationship; The output unit is used to obtain the ion intensity according to the conversion results output by the first detector and the calculation unit.

4. The mass spectrometry data acquisition method based on multi-channel technology according to claim 3, characterized in that: The first detector is a microchannel plate detector, the second detector is a Faraday cup, and the third detector is a charge receiving plate.

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