Large yellow tea polysaccharide with anti-inflammatory activity, preparation method and application of large yellow tea polysaccharide and anti-inflammatory pharmaceutical composition

A composition and big tea technology, applied in the direction of drug combination, organic active ingredients, medical preparations containing active ingredients, etc., can solve the lack of anti-inflammatory active polysaccharides, unclear chemical composition and structure of anti-inflammatory active polysaccharides of Huoshan yellow big tea, Restrict research and development and other issues to achieve the effect of novel structure, environmental friendliness and maintaining activity

CN114751997AActive Publication Date: 2022-07-15ANHUI AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2022-07-15

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Abstract

The invention provides a large yellow tea polysaccharide with anti-inflammatory activity, a preparation method and application of the large yellow tea polysaccharide and an anti-inflammatory pharmaceutical composition, and belongs to the technical field of application of active substances of large yellow tea. The large yellow tea polysaccharide with the anti-inflammatory activity is novel in structure, has a definite chemical structure, is uniform in composition, has an obvious anti-inflammatory function, can remarkably reduce expression of inflammatory factors in bone marrow-derived macrophages caused by lipopolysaccharide, and has no toxic or side effect on normal macrophages. As the polysaccharide with natural anti-inflammatory activity, the problem that the Huangdan tea lacks an active component with a single composition and a clear structure in the aspect of research and development of new anti-inflammatory drugs is solved, and a reliable material basis is laid for research on the structure-function relationship of the anti-inflammatory action of the Huangdan tea polysaccharide and research and development of the anti-inflammatory drugs.
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Description

technical field

[0001] The invention relates to the technical field of application of active substances in yellow tea, in particular to a yellow tea polysaccharide with anti-inflammatory activity, a preparation method and application thereof, and an anti-inflammatory pharmaceutical composition. Background technique

[0002] Tea (Camellia sinensis) is a perennial evergreen plant of the Camellia family Camellia. Tea made from its leaves and twigs through a special process enjoys unique flavor charm, diverse health care functions, and profound cultural heritage, and is popular all over the world. It has become one of the three major non-alcoholic beverages in the world, and its consumption is second only to drinking water. Literature records that tea has been used as a medicinal product for thousands of years. Drinking tea has the effects of "quenching thirst, digesting food, removing phlegm, reducing sleep, diuretic, improving eyesight, improving thinking, eliminating vexation...

Examples

preparation example Construction

[0036] like figure 1 As shown, the present invention provides the preparation method of the yellow tea polysaccharide with anti-inflammatory activity described in the above technical scheme, comprising the following steps:

[0037] After decolorizing and degreasing Huoshan Huangda tea, Huoshan Huangdacha powder is obtained;

[0038] Mixing the Huoshan huang da tea powder with water, and performing water extraction to obtain a huang da tea water extract;

[0039] Mixing the Huangda tea water extract with ethanol, and carrying out alcohol precipitation to obtain the Huangda tea alcohol deposit;

[0040] Deproteinization and dialysis are carried out successively to the alcohol precipitate of Huangdacha, to obtain a Huangdacha polysaccharide extract;

[0041] The polysaccharide extract of Huang Da tea is subjected to anion exchange column chromatography, and the polysaccharide-containing eluent is collected to obtain the extract of Huang Da tea polysaccharide;

[0042] Perform ...

Embodiment 1

[0064] (1) Dry Huoshan Huangdacha at 60°C, pulverize, and sieve with 80 mesh; add 95% ethanol according to the material-to-liquid ratio of 1g:20mL, stir at 25°C and 50rpm / min for 24h, filter with suction, collect Precipitation to obtain decolorized and degreasing Huoshan Huangda tea powder;

[0065] (2) Mix the decolorized and degreasing Huoshan Huangda tea powder in step (1) with distilled water in a ratio of 1g:20mL, stir and extract at 90°C and 50rpm / min for 2.5h, filter with suction, collect the filtrate, repeat 2 times , merged 3 filtrates to obtain Huoshan Huangda tea water extract;

[0066] (3) Concentrate the water extract of Huoshan Huangda tea in step (2) under reduced pressure to 1 / 3 of the original volume at 65°C and 0.10MPa vacuum degree, centrifuge at 4°C and 10000rpm / min for 20min, discard the precipitation, and collect the Anhydrous ethanol was added to the supernatant to make the final concentration of ethanol to be 80%. After 24 hours of precipitation at 4°C...