Magnetic stirring device

Abstract

The present invention provides a magnetic stirring device, which is used in conjunction with a strip reaction chamber, and the magnetic stirring device includes a substrate, on which a rotating driving component is installed, and the magnetic stirring device also includes a magnetic module, and the magnetic module includes a first magnetic component and a second magnetic component, wherein the first magnetic component can move under the drive of the rotating driving component, and the second magnetic component is in the strip reaction chamber and can rotate in the strip reaction chamber by relying on the suction between it and the first magnetic component. The rotating driving component of the present invention drives the first magnetic component in the magnetic module to rotate, and drives the movement in the second magnetic component through the suction between it and the second magnetic component, and can achieve stirring and mixing of the reaction liquid in the strip reaction chamber without contact with the chip bottom plate, which can reduce the reaction time in the entire microfluidic chip, improve process performance, reduce process steps, and improve reaction efficiency.

Description

Technical Field

[0001] The invention belongs to the technical field of microfluidic chip design, and in particular relates to a magnetic stirring device. Background Art

[0002] Stirring equipment is widely used in industrial production, especially in chemical production. Almost all chemical departments have more or less stirring operations. Even in the rapidly developing polymer work in recent years, 90% of the polymerization reactors as the core equipment for producing polymer materials are stirring equipment. Stirring operation as the basic unit operation of process operation is an important link in the chemical reaction process. Its principle involves multiple processes such as fluid mechanics, heat transfer, mass transfer and chemical reaction. The amplification process is the process of momentum transfer in the flow field or including momentum, heat, mass transfer and chemical reaction. The stirrer is a mechanical device that uses the rotation of the stirring blade to input mechanical energy to the fluid so that the fluid obtains a suitable flow field. Some stirring mechanisms such as turbine stirrers, paddle stirrers, anchor stirrers, etc., although they realize the stirring function, their system is complex, expensive, and too large in size, and are not suitable for large-scale promotion. In order to speed up the reaction speed and reduce the reaction time of microfluids, a magnetic stirring device with simple operation, simple system and effective is very needed. Summary of the invention

[0003] Therefore, the technical problem to be solved by the present invention is to provide a magnetic stirring device for use with a microfluidic chip, which can stir and mix the reaction liquid in the strip reaction chamber without contact with the chip base plate, thereby reducing the reaction time in the entire microfluidic system, improving process performance, reducing process steps, and improving chip reaction efficiency.

[0004] In order to solve the above problems, the present invention provides a magnetic stirring device, which is used in conjunction with a strip-shaped reaction chamber. The magnetic stirring device includes a substrate, on which a rotating driving component is installed. The magnetic stirring device also includes a magnetic module, and the magnetic module includes a first magnetic member and a second magnetic member, wherein the first magnetic member can move under the drive of the rotating driving component, and the second magnetic member is in the strip-shaped reaction chamber and can rotate in the strip-shaped reaction chamber by relying on the suction force between the second magnetic member and the first magnetic member.

[0005] In some embodiments, the magnetic stirring device is used in conjunction with a microfluidic chip, and the microfluidic chip includes a chip base plate, and a strip-shaped reaction chamber is constructed on the chip base plate.

[0006] In some embodiments, the magnetic module also includes a magnetic component mounting component, which is a bar structure, and the length of the bar structure is not less than the length of the bar reaction chamber. The magnetic component mounting component is connected to the rotating shaft of the rotating drive component, and the rotating shaft is connected to the middle position of the length of the magnetic component mounting component, and the first magnetic component is arranged at one end of the length of the magnetic component mounting component.

[0007] In some embodiments, the magnetic component mounting member has a first placement groove, and the first magnetic component is located in the first placement groove.

[0008] In some embodiments, the magnetic component mounting member also has a second placement groove, the first placement groove and the second placement groove are respectively located at the two ends of the length of the magnetic component mounting member and are symmetrical about the axis of the rotating shaft, and a balancing member is provided in the second placement groove, and the balancing member can form a torque balance with the first magnetic member in the length direction of the magnetic component mounting member.

[0009] In some embodiments, the balance member is made of non-magnetic material.

[0010] In some embodiments, the chip base plate has a plurality of strip-shaped reaction chambers, and the rotation drive components and the magnetic modules have a plurality of corresponding ones, and the plurality of rotation drive components, the magnetic modules and the strip-shaped reaction chambers are respectively arranged in one-to-one correspondence.

[0011] In some embodiments, the magnetic stirring device further comprises a driving component fixing member, and the driving component fixing member is configured with mounting holes corresponding one-to-one to the plurality of rotating driving components.

[0012] In some embodiments, a counterweight plate is further provided on a side of the substrate away from the magnetic module.

[0013] In some embodiments, the microfluidic chip is a microfluidic nucleic acid detection chip.

[0014] The present invention provides a magnetic stirring device for use with a strip-shaped reaction chamber. The rotating driving component drives the first magnetic component in the magnetic module to rotate, and then drives the movement of the second magnetic component through the suction between the first magnetic component and the second magnetic component. In this way, the reaction liquid in the strip-shaped reaction chamber can be stirred and mixed without contact with the chip bottom plate, which can reduce the reaction process time of the entire microfluidic chip, improve process performance, reduce process steps, and improve chip reaction efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 A schematic diagram of the three-dimensional structure of a magnetic stirring device used in conjunction with a microfluidic chip according to an embodiment of the present invention (the second magnetic member is omitted);

[0016] Figure 2 for Figure 1 Schematic diagram of the exploded structure of the magnetic stirring device;

[0017] Figure 3 It is a schematic diagram of the exploded structure of a microfluidic chip matched with the magnetic stirring device of the present invention;

[0018] Figure 4 The state of the magnetic stirring device of the present invention and the chip base plate (microfluidic chip) when they are used together;

[0019] Figure 5 for Figure 4 A top view of the chip base plate in FIG.

[0020] The reference numerals are as follows:

[0021] 1. Base plate; 2. Rotating driving component; 31. First magnetic component; 32. Second magnetic component; 33. Magnetic component mounting component; 331. First placement slot; 332. Second placement slot; 34. Balance component; 4. Driving component fixing component; 5. Counterweight plate; 101. First piston push rod; 102. Second piston push rod; 103. Chip front shell; 104. Rubber plug; 105. First sealing plug; 106. Second sealing plug; 107. Chip rear shell; 108. Reagent storage layer; 109. Color development area; 110. Elastic tape layer; 201. Chip bottom plate; 202. Strip reaction chamber. DETAILED DESCRIPTION

[0022] See also Figure 1 and Figure 5As shown, according to an embodiment of the present invention, there is provided a magnetic stirring device for use with a microfluidic chip, comprising a substrate 1, on which a rotating driving component 2 (specifically, a reduction motor) is mounted, the magnetic stirring device further comprising a magnetic module, the microfluidic chip comprising a chip base plate 201, on which a strip-shaped reaction chamber 202 is constructed, the magnetic module comprising a first magnetic member 31 (e.g., a magnet), and a second magnetic member 32 (e.g., another magnet), wherein the first magnetic member 31 can move under the drive of the rotating driving component 2, the second magnetic member 32 is in the strip-shaped reaction chamber 202 and can rotate in the strip-shaped reaction chamber 202 by relying on the suction between the first magnetic member 31 and the first magnetic member 31. In this embodiment, the microfluidic chip is a microfluidic nucleic acid detection chip. In this technical solution, the rotating driving component 2 drives the first magnetic component 31 in the magnetic module to rotate, and then drives the movement of the second magnetic component 32 through the suction between it and the second magnetic component 32, so that the reaction liquid in the bar-shaped reaction chamber 202 can be stirred and mixed without contact with the chip bottom plate 201, which can reduce the time of the entire nucleic acid detection process, improve process performance, reduce process steps, and improve detection efficiency; in addition, it should be specially noted that the use of a magnetic stirring device only requires the corresponding second magnetic component 32 to be placed in the bar-shaped reaction chamber 202 of the existing microfluidic chip, without a large number of improvements to the structure of the chip itself, thereby reducing costs. At the same time, the magnetic stirring device in this technical solution is used in conjunction with the microfluidic chip, and is small in size and light in weight.

[0023] In some embodiments, the magnetic module also includes a magnetic component mounting member 33, which is a bar structure, and the length of the bar structure is not less than the length of the bar reaction chamber 202, the magnetic component mounting member 33 is connected to the rotating shaft of the rotating drive component 2, the rotating shaft is connected to the middle position of the length of the magnetic component mounting member 33, and the first magnetic component 31 is arranged at one end of the length of the magnetic component mounting member 33, so that the first magnetic component 31 forms an offset distance with the axis of the rotating shaft. In this technical solution, by designing the magnetic component mounting member 33 to be a bar structure with a length greater than the length of the corresponding strip reaction chamber 202, and offsetting the first magnetic component 31, when the rotating driving component 2 drives the magnetic component mounting member 33 to rotate, the first magnetic component 31 can drive the second magnetic component 32 to rotate in the strip reaction chamber 202 and also form a reciprocating motion along the length direction of the strip reaction chamber 202, so that the reaction liquid in the strip reaction chamber 202 is stirred more thoroughly, the mixing effect is further improved, and the detection efficiency is correspondingly further improved.

[0024] In some embodiments, the magnetic component mounting member 33 has a first placement groove 331, and the first magnetic component 31 is located in the first placement groove 331. Setting the first magnetic component 31 in the first placement groove 331 can ensure the position reliability and stability of the first magnetic component 31 and prevent the first magnetic component 31 from falling off during the rotation of the magnetic component mounting member 33.

[0025] Preferably, the magnetic component mounting member 33 also has a second placement groove 332, the first placement groove 331 and the second placement groove 332 are respectively located at the two ends of the length of the magnetic component mounting member 33 and are symmetrical about the axis of the rotating shaft, and a balancing member 34 is provided in the second placement groove 332, and the balancing member 34 can form a torque balance with the first magnetic member 31 in the length direction of the magnetic component mounting member 33. In this way, through the symmetrical design of the balancing member 34 and the first magnetic member 31, the overall operation balance of the magnetic component mounting member 33 is ensured, and the vibration of the device during operation is effectively prevented.

[0026] The material of the balancing member 34 is a non-magnetic material, such as stainless steel. The use of non-magnetic material can prevent adverse interference with the magnetism of the first magnetic member 31 when achieving its balancing purpose, thereby adversely affecting the suction between the second magnetic member 32, thereby ensuring the smooth operation of the second magnetic member 32.

[0027] In some embodiments, the chip base plate 201 has multiple (specifically, two) strip-shaped reaction chambers 202, and the rotation drive components 2 and the magnetic modules have multiple corresponding ones. The multiple rotation drive components 2, the magnetic modules and the strip-shaped reaction chambers 202 are respectively arranged in a one-to-one correspondence. In this way, a set of the magnetic stirring device can simultaneously stir multiple strip-shaped reaction chambers 202, thereby further improving the detection efficiency.

[0028] The magnetic stirring device also includes a driving component fixing member 4, on which mounting holes corresponding to the plurality of rotating driving components 2 are constructed. In this way, the plurality of rotating driving components 2 can be fixed separately and simultaneously, making the device structure more compact and also protecting the internal rotating driving components 2.

[0029] A counterweight plate 5 is also provided on one side of the base plate 1 away from the magnetic module, which can be bolted to the bottom surface of the base plate 1 to prevent vibration of the device during operation, ensure the stability of the device during operation, and reduce operating noise.

[0030] See also Figure 3As shown, the structure of the microfluidic chip used in the magnetic stirring device of the present invention specifically includes a chip front shell 103 and a chip rear shell 107, both of which protect and support the internal chip as a whole, and the first piston push rod 101 and the second piston push rod 102 are provided with a rubber plug 104 under them, which can promote the reagent to flow in the reagent storage layer 108, and the rubber plug 104 seals the chip after adding the sample. The first sealing plug 105 and the second sealing plug 106 seal the area of ​​the reagent storage layer 108, and the reagent storage layer 108 has a reagent storage chamber to store reagents, wherein there are two for providing push rod activity space. An elastic tape layer 110 is arranged between the bottom of the reagent storage layer 108 and the chip base plate 201, and it selects a double-sided adhesive substrate to bond the chip base plate 201 and the reagent storage layer 108 to encapsulate the chip. The reagent flow direction is controlled by a local one-way valve to cooperate with the chip base plate 201 to carry out directional micro-operation on the reagent. The chip base plate 201 has a plurality of strip-shaped reaction chambers 202, which can perform micro-manipulation on reagents and provide a nucleic acid amplification chamber for amplifying nucleic acids. The color development area 109 has a color development test paper, which can present the final test result.

[0031] The operation process of the microfluidic chip is as follows: add the sample to the sample hole, insert the rubber plug 104, and the sample will enter the corresponding chamber. First, pull up the first piston push rod 101, and the sample liquid will enter the chamber of the chip base plate 201 from the aforementioned chamber through the one-way valve of the elastic tape layer 110. The reagent is heated and stirred in the chamber at the same time, and a chemical reaction occurs. Then push the first piston push rod 101 downward and the liquid passes through the one-way valve of the elastic tape layer 110 to enter another storage chamber of the reagent storage layer. Then push the second piston push rod 102 upward and the liquid enters another chamber of the chip base plate 201 from the aforementioned storage chamber through the one-way valve of the elastic tape layer 110. The reagent is heated and stirred in the other chamber, and a chemical reaction occurs. Then push the second piston push rod 102 downward and the liquid passes through the one-way valve of the elastic tape layer 110 to enter the waste liquid area (recovery chamber) and the color development area 109. The test strip in the color development area 109 begins to develop color when it encounters the reagent. Read the test strip color status and determine the result.

[0032] It is easy for those skilled in the art to understand that, under the premise of no conflict, the above-mentioned advantageous methods can be freely combined and superimposed.

[0033] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention shall be included in the protection scope of the present invention. The above are only preferred embodiments of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and variations can be made without departing from the technical principles of the present invention, and these improvements and variations should also be regarded as the protection scope of the present invention.

Claims

1. A structure consisting of a microfluidic chip and a magnetic stirring device, It is characterized in that The invention comprises the microfluidic chip and the magnetic stirring device, wherein the magnetic stirring device comprises a substrate (1), a rotating driving component (2) is mounted on the substrate (1), and the magnetic stirring device further comprises a magnetic module, wherein the magnetic module comprises a first magnetic component (31) and a second magnetic component (32), wherein the first magnetic component (31) can move under the drive of the rotating driving component (2), and the second magnetic component (32) is located in a strip-shaped reaction chamber and can rotate in the strip-shaped reaction chamber by virtue of the suction force between the first magnetic component (31) and the first magnetic component (32); the magnetic stirring device is used to rotate with the first magnetic component (31) The microfluidic chip is used in conjunction with the microfluidic chip, the microfluidic chip is a microfluidic nucleic acid detection chip, the microfluidic chip comprises a chip base plate (201), a bar-shaped reaction chamber (202) is constructed on the chip base plate (201), the length of the bar-shaped reaction chamber (202) is greater than its width, and the magnetic stirring device is used in conjunction with the bar-shaped reaction chamber; the magnetic module also comprises a magnetic component mounting member (33), the magnetic component mounting member (33) is a bar-shaped structure, and the length of the bar structure is not less than the length of the bar-shaped reaction chamber (202), and the magnetic component mounting member (33) is connected to the rotation drive unit. The first magnetic component (31) is connected to the rotating shaft of the magnetic component mounting component (2), the rotating shaft is connected to the middle position of the length of the magnetic component mounting component (33), and the first magnetic component (31) is arranged at one end of the length of the magnetic component mounting component (33), so that the first magnetic component (31) can drive the second magnetic component (32) to rotate in the strip-shaped reaction chamber (202) and can also form a reciprocating motion along the length direction of the strip reaction chamber (202); the magnetic component mounting component (33) has a first placement groove (331), and the first magnetic component (31) is located in the first placement groove (331); the magnetic component mounting component (33) The mounting member (33) also has a second placement groove (332), the first placement groove (331) and the second placement groove (332) are respectively located at the two ends of the length of the magnetic member mounting member (33) and are symmetrical about the axis of the rotating shaft, and a balancing member (34) is provided in the second placement groove (332), and the balancing member (34) can form a torque balance with the first magnetic member (31) in the length direction of the magnetic member mounting member (33); the material of the balancing member (34) is a non-magnetic material; there are multiple strip-shaped reaction chambers (202) on the chip base plate (201).

2. The structure consisting of a microfluidic chip and a magnetic stirring device according to claim 1, It is characterized in that The rotation drive component (2) and the magnetic module are provided in plurality, and the rotation drive components (2), the magnetic modules and the strip-shaped reaction chamber (202) are arranged in a one-to-one correspondence.

3. The structure consisting of a microfluidic chip and a magnetic stirring device according to claim 2, It is characterized in that It also comprises a drive component fixing member (4), on which mounting holes corresponding one to one with the plurality of rotation drive components (2) are formed.

4. The structure consisting of a microfluidic chip and a magnetic stirring device according to claim 1, It is characterized in that A counterweight plate (5) is also provided on a side of the base plate (1) away from the magnetic module.

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