A disposable fluorescent immunochromatographic detection kit

Abstract

This invention provides a disposable fluorescence immunochromatographic assay kit, comprising a housing, a light source assembly, and a reagent card housed within the housing. The reagent card has a detection window and a sample dispensing port. The housing is a closed shell structure made of flexible light-shielding material. The reagent card is located at the bottom of the housing. The light generated by the light source assembly illuminates the detection window of the reagent card. An observation window and a dispensing port are located at the top of the housing. The observation window corresponds to the detection window, and the dispensing port corresponds to the sample dispensing port. This disposable fluorescence immunochromatographic assay kit allows for rapid self-testing, is suitable for home and portable testing, has high reliability, a compact and rationally designed overall structure, low production cost, and is suitable for large-scale production.

Description

Technical Field

[0001] The present invention relates to the technical field of external diagnostic testing, and in particular to a self-service testing box. Background Art

[0002] Immunochromatographic Assay (ICA) is an analytical method developed in the late 20th century that combines immunoassay and chromatographic techniques. This method has the characteristics of specificity, simple operation, and rapidity, and is widely used in important fields such as clinical diagnosis, environmental monitoring, and food safety. Traditional immunochromatographic technology uses colloidal gold as a marker and performs qualitative detection or semi-quantitative analysis of the target through strip color development. Although this method is simple and fast, it has poor sensitivity and is difficult to accurately quantify. As a new type of immunoassay technology, fluorescent immunochromatographic technology retains the advantages of rapid on-site detection of traditional colloidal gold test strips, and adds the high sensitivity of fluorescent detection technology, becoming one of the main ways to improve the detection performance of immunochromatographic methods.

[0003] Fluorescent immunochromatography is a new membrane detection technology based on antigen-antibody specific immune reactions. This technology uses a strip of fiber chromatography material fixed with a test line (coated antibody or coated antigen) and a quality control line (antibody) as the stationary phase, the test liquid as the mobile phase, and the fluorescently labeled antibody or antigen fixed to the connection pad. The analyte is moved on the chromatography strip by capillary action. For large molecular antigens with multiple antigenic determinants (proteins, viruses, pathogenic bacteria, etc.), a "sandwich" type double antibody sandwich immunochromatography method is usually used. That is, the analyte first binds to the fluorescently labeled antibody under the action of the mobile phase, and then binds to the coated antibody when it reaches the test line to form a double antibody sandwich type "sandwich". After the antigen-antibody reaction, the fluorescence intensity can be measured using special instruments to infer the concentration of the analyte.

[0004] Unlike traditional immunoassays, immunofluorescence testing requires instrumentation and is therefore primarily used in medical institutions and testing labs, rarely at home. As viruses and bacteria continue to pose challenges to human health, large hospitals are overcrowded, medical care is difficult to access, and testing times are lengthy. Rapid and accurate screening for infectious diseases has become a pressing challenge for the industry. Summary of the Invention

[0005] The technical problem to be solved by the present invention is to overcome the above-mentioned defects of the known technology and provide a disposable fluorescent immunochromatographic detection kit that can realize personal rapid self-service detection, is suitable for home and portable detection, has good reliability, and has a compact overall structure, reasonable layout, low production cost, and is conducive to large-scale production and processing.

[0006] The present invention is achieved through the following technical solutions. A disposable fluorescent immunochromatographic detection kit provided by the present invention includes a box body and a light source assembly and a reagent card placed in the box body. The reagent card has a detection window and a sample addition hole, wherein the box body is a closed shell structure made of flexible light-shielding material; the reagent card is arranged at the bottom of the box body, and the light generated by the light source assembly is used to illuminate the detection window of the reagent card. An observation window and a liquid injection hole are opened on the top of the box body, wherein the observation window corresponds to the detection window, and the liquid injection hole corresponds to the sample addition hole.

[0007] By adopting the above structure, the convenience of visual reading is improved by arranging a light source component in the box body. The box body adopts flexible light-shielding material to avoid the interference of ambient light on the reading of test results, and reduces the weight of the box body, making it more convenient to carry; the closed shell structure can reduce the pollution to the environment when discarded after single use. This test kit has a simple structure and is easy to operate, which improves the usability of the product.

[0008] The present invention can also be further implemented by taking the following technical solutions:

[0009] The aforementioned disposable fluorescent immunochromatographic test kit, wherein the light source assembly includes a power supply, a button, an LED unit, and an LED control unit, wherein the LED unit includes one or more LEDs. Preferably, a filter is provided above the LED unit. The filter can effectively filter out stray light from ambient light, reducing interference.

[0010] Preferably, in the aforementioned disposable fluorescent immunochromatographic test kit, the LED control unit includes a constant current drive circuit, wherein a first input of the constant current drive circuit is connected to an output of the power supply, and an output of the constant current drive circuit is connected to the LED unit to maintain a constant load current in the LED unit. By providing a constant current drive, the brightness of multiple LEDs or LEDs from different batches is ensured to be consistent, resulting in greater light source consistency and more stable illumination, further ensuring visual accuracy.

[0011] Preferably, in the aforementioned disposable fluorescent immunochromatographic test kit, the LED control unit further includes an RC delay circuit, the input of which is connected to the power supply via a key, and the output of which is connected to the second input of the constant current drive circuit. When the key is released, the RC delay circuit begins to discharge, and after a preset delay period, the constant current drive circuit stops outputting current, turning off the LED unit. Adding a delayed shutoff function facilitates visual reading or photography by the user and prevents light pollution.

[0012] The aforementioned disposable fluorescent immunochromatographic detection kit, wherein the constant current drive circuit includes a WD3139 constant current drive chip U1, an inductor L1, a freewheeling diode D20, a resistor R3, a capacitor C1 and a capacitor C2; the 6 pins of the U1 are divided into three paths, the first path is connected to the first end of the capacitor C1, the second path is connected to the first end of the inductor L1, and the third path is connected to the positive electrode of the power supply; the second end of the capacitor C1 is grounded; the 1 pin of the U1 is divided into two paths, the first path is connected to the second end of the inductor L1, the second path is connected to the positive electrode of the freewheeling diode D20, and the negative electrode of the freewheeling diode D20 is divided into three paths, the first path is connected to the 5 pin of U1, the second path is connected to the first end of the capacitor C2, and the third path is connected to the positive electrode of the LED; the second end of the capacitor C2 is grounded; the 3 pins of the U1 are divided into two paths, the first path is connected to the negative electrode of the LED, and the second path is grounded through the resistor R3; the 2 pins of the U1 are grounded.

[0013] Preferably, the aforementioned disposable fluorescent immunochromatographic detection kit, wherein the light source assembly is integrated on a control circuit board, the control circuit board is provided with a first window and a second window, the first window corresponds to the observation window, and the second window corresponds to the injection hole; the LED is arranged along the edge of the first window.

[0014] Furthermore, the aforementioned disposable fluorescent immunochromatographic detection kit, wherein the box body includes an upper shell and a lower shell, the upper shell and the lower shell are relatively buckled to form a hollow shell structure with a closed circumference, the reagent card is fixed to the inner bottom of the lower shell, and the control circuit board is fixed to the inner top of the upper shell.

[0015] Furthermore, the aforementioned disposable fluorescent immunochromatographic detection kit, wherein the box body is folded by a bendable flexible material, including a front plate, a top plate, a back plate and a bottom plate connected in sequence, a first connecting plate is formed at the lower end of the bottom plate, a first side plate is formed on both sides of the top plate, a second side plate is formed on both sides of the bottom plate, a second connecting plate is formed on both sides of the front plate, and a third connecting plate is formed on both sides of the back plate; the reagent card is fixed to the inner side surface of the bottom plate, and the control circuit board is fixed to the inner side surface of the top plate; a fold is formed at the connection between the front plate, the top plate, the back plate, the bottom plate and the first connecting plate, and the first connecting plate is pasted on the inner side of the front plate after being bent along the fold; a fold is formed at the connection between the front plate and the second connecting plate, the top plate and the first side plate, the back plate and the third connecting plate, and the bottom plate and the second side plate, and the first side plate is pasted on the outer side of the second side plate through the second connecting plate and the third connecting plate after being bent along the fold.

[0016] Preferably, in the aforementioned disposable fluorescent immunochromatographic detection kit, at least one support member is arranged between the control circuit board and the reagent card, and the bottom end face of the support member is fixed to the end face of the reagent card and / or the top end face of the support member is fixed to the end face of the control circuit board.

[0017] Preferably, in the aforementioned disposable fluorescent immunochromatographic test kit, the control circuit board and the reagent card are fixedly connected by a support member to form a core assembly. This structure facilitates assembly and improves production efficiency.

[0018] Preferably, in the aforementioned disposable fluorescent immunochromatographic detection kit, the box body includes an upper shell and a lower shell, the movement assembly is placed in the lower shell, and the upper shell is buckled onto the outer side of the lower shell.

[0019] Preferably, the aforementioned disposable fluorescent immunochromatographic detection kit, wherein the box body is formed of a bendable flexible material to form a hollow shell structure with one end open, the opening extending to form an upper cover plate, a lower cover plate, a first folding plate, and a second folding plate, the movement is always placed in the box body from the opening, and the upper cover plate, the lower cover plate, the first folding plate, and the second folding plate are folded to close the opening.

[0020] Preferably, in the aforementioned disposable fluorescent immunochromatographic test kit, the height of the box body is 9.5±5 mm. This height ensures optimal visual effect and avoids uneven light spots caused by being too close, and insufficient brightness or increased power consumption and product size caused by being too far away.

[0021] Preferably, in the aforementioned disposable fluorescent immunochromatographic test kit, the detection window of the reagent card is provided with fluorescent markers on both sides, with two on one side and one on the other side. When a user takes a photo with a mobile phone, the fluorescent markers can facilitate camera positioning.

[0022] Preferably, in the aforementioned disposable fluorescent immunochromatographic test kit, the housing can be made of black cardboard, black cardboard, silicone, TPU, TPC, or PVC. In addition to providing light-blocking properties, these materials are lightweight, reducing the weight of the box, making it more portable. Furthermore, the buttons are wrapped within the box, maintaining their functionality thanks to the elastic properties of the material, eliminating the need for separate openings in the box, ensuring better light-blocking properties and a simpler appearance.

[0023] In summary, compared with the prior art, the disposable fluorescent immunochromatographic detection kit of the present invention can realize personal rapid self-service detection, is suitable for home and portable detection, has good reliability, and has a compact overall structure, reasonable layout, low production cost, and is conducive to large-scale production and processing. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 Schematic diagram of the appearance of the disposable fluorescent immunochromatographic detection kit of the present invention;

[0025] Figure 2 Schematic diagram of the structure of the reagent card of the present invention;

[0026] Figure 3 is a structural schematic diagram of a light source assembly of the present invention;

[0027] Figure 4 A circuit diagram of a light source assembly of the present invention;

[0028] Figure 5 This is a schematic diagram of the assembly of the first embodiment of the present invention;

[0029] Figure 6 A partial cross-sectional view of the first embodiment of the present invention;

[0030] Figure 7 This is an unfolded front view of the box body of Example 2 of the present invention;

[0031] Figure 8 This is an unfolded back view of the box body of the second embodiment of the present invention;

[0032] Figure 9 This is an assembly diagram of embodiment 3 of the present invention;

[0033] Figure 10 This is an assembly diagram of embodiment 4 of the present invention. DETAILED DESCRIPTION

[0034] The structure, features and effects of the present invention are further described in detail below with reference to the accompanying drawings and preferred embodiments.

[0035] It should be noted that identical or corresponding parts are denoted by the same reference numerals in the drawings, and repeated descriptions are omitted. Terms such as "upper," "lower," "front," and "rear" indicating positions or relationships are based on the positions or relationships shown in the drawings and are used solely to facilitate and simplify the description of the present invention. They are not limited to a single position or spatial orientation and should not be construed as limitations on the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the quantity of the indicated technical features.

[0036] like Figures 1 to 4As shown, the present invention provides a disposable fluorescent immunochromatographic detection kit, comprising a box body 1 and a light source assembly and a reagent card 3 placed in the box body, wherein the reagent card has a detection window 31 and a sample addition hole 32, wherein the box body 1 is a closed shell structure made of a flexible light-shielding material; the reagent card is arranged at the bottom of the box body, and the light generated by the light source assembly is used to illuminate the detection window 31 of the reagent card. An observation window 4 and an injection hole 5 are provided at the top of the box body 1, wherein the observation window 4 corresponds to the detection window 31, and the injection hole 5 corresponds to the sample addition hole 32.

[0037] By adopting the above structure, the convenience of visual reading is improved by arranging a light source component in the box body. The box body adopts flexible light-shielding material to avoid the interference of ambient light on the reading of test results, and reduces the weight of the box body, making it more convenient to carry; the closed shell structure can reduce the pollution to the environment when discarded after single use. This test kit has a simple structure and is easy to operate, which improves the usability of the product.

[0038] This embodiment can be further implemented through the following structure:

[0039] In the aforementioned disposable fluorescent immunochromatographic detection kit, the light source assembly includes a power supply 21, a button S1, an LED unit 23 and an LED control unit, and the LED unit 23 includes one or more LEDs.

[0040] Preferably, in the aforementioned disposable fluorescent immunochromatographic test kit, the LED control unit includes a constant current drive circuit 24, wherein a first input terminal of the constant current drive circuit is connected to an output terminal of the power supply, and an output terminal of the constant current drive circuit is connected to the LED unit to maintain a constant load current of the LED unit. By providing a constant current drive, the brightness of multiple LEDs or LEDs from different batches is ensured to be consistent, thereby improving the consistency of the light source and stabilizing the illumination, further ensuring the accuracy of visual inspection.

[0041] Preferably, in the aforementioned disposable fluorescent immunochromatographic test kit, the LED control unit further includes an RC delay circuit 25. The input of the RC delay circuit is connected to the power supply via a button, and the output of the RC delay circuit is connected to the second input of the constant current drive circuit. When the button is disconnected, the RC delay circuit begins to discharge. After a preset delay period, the constant current drive circuit stops outputting current, and the LED unit turns off. Adding a delayed shutoff function facilitates visual reading or photography by the user and prevents light pollution. For example, the delay period can be set to 30 seconds to 3 minutes.

[0042] The aforementioned disposable fluorescent immunochromatographic detection kit, wherein the constant current drive circuit 24 includes a WD3139 constant current drive chip U1, an inductor L1, a freewheeling diode D20, a resistor R3, a capacitor C1 and a capacitor C2; the 6 pins of the U1 are divided into three paths, the first path is connected to the first end of the capacitor C1, the second path is connected to the first end of the inductor L1, and the third path is connected to the positive electrode of the power supply; the second end of the capacitor C1 is grounded; the 1 pin of the U1 is divided into two paths, the first path is connected to the second end of the inductor L1, the second path is connected to the positive electrode of the freewheeling diode D20, and the negative electrode of the freewheeling diode D20 is divided into three paths, the first path is connected to the 5 pin of U1, the second path is connected to the first end of the capacitor C2, and the third path is connected to the positive electrode of the LED; the second end of the capacitor C2 is grounded; the 3 pins of the U1 are divided into two paths, the first path is connected to the negative electrode of the LED, and the second path is grounded through the resistor R3; the 2 pins of the U1 are grounded.

[0043] Better, such as Figure 5 、 Figure 6 As shown, the aforementioned disposable fluorescent immunochromatographic detection kit, wherein the light source assembly is integrated on the control circuit board 2, and the control circuit board is provided with a first window 26 and a second window 27, the first window 26 corresponds to the observation window 4, and the second window 27 corresponds to the injection hole 5; the LEDs are arranged along the edge of the first window. Preferably, 4-8 LEDs can be provided. Preferably, a filter 6 is provided above the LED unit, which can effectively filter out stray light in the ambient light and reduce interference. For example, the filter 6 can be attached to the back side of the control circuit board 2 and cover the first window 26.

[0044] The box body can adopt the following specific structure:

[0045] Example 1

[0046] like Figure 5 、 Figure 6 As shown, the box body 1 includes an upper shell 11 and a lower shell 12. The upper shell and the lower shell are relatively buckled to form a hollow shell structure with a closed periphery. The reagent card 3 is fixed to the inner bottom of the lower shell 12, and the control circuit board 2 is fixed to the inner top of the upper shell 11. Specifically, the connection between the upper shell and the lower shell can be fixed with glue, double-sided tape, etc. The reagent card and control circuit board can be fixed with adhesive backing. Accordingly, the top of the upper shell is provided with an observation window 4 and a liquid injection hole 5.

[0047] Example 2

[0048] like Figure 7 、 Figure 8As shown, the box body is folded by a bendable flexible material and includes a front plate 101, a top plate 102, a back plate 103 and a bottom plate 104 connected in sequence. A first connecting plate 1041 is formed at the lower end of the bottom plate 104, first side plates 1021 are formed on both sides of the top plate 102, second side plates 1042 are formed on both sides of the bottom plate, second connecting plates 1011 are formed on both sides of the front plate 101, and third connecting plates 1031 are formed on both sides of the back plate 103; the reagent card 3 is fixed to the inner side of the bottom plate 104 The control circuit board 2 is fixed to the inner side of the top plate 102; the connection between the front plate, top plate, back plate, bottom plate, and first connecting plate is formed with a crease, and after bending along the crease, the first connecting plate 1041 is attached to the inner side of the front plate 101; the connection between the front plate and the second connecting plate, the top plate and the first side plate, the back plate and the third connecting plate, and the bottom plate and the second side plate is formed with a crease, and after bending along the crease, the first side plate is attached to the outer side of the second side plate through the second connecting plate and the third connecting plate. The reagent card and control circuit board can be fixed by adhesive. Correspondingly, the top plate 102 is provided with an observation window 4 and a liquid injection hole 5.

[0049] Preferably, in the aforementioned disposable fluorescent immunochromatographic test kit, at least one support member 7 is disposed between the control circuit board and the reagent card, with the bottom end face of the support member 7 being fixed to the end face of the reagent card 3 and / or the top end face of the support member 7 being fixed to the end face of the control circuit board 2. The support member can be made of EVA. Providing the support member prevents component displacement or dislodging caused by compression of the box body and ensures a fixed distance between the light source assembly and the reagent card.

[0050] Further, such as Figure 9 As shown, in the aforementioned disposable fluorescent immunochromatographic test kit, the control circuit board 2 and the reagent card 3 are fixedly connected by a support member 7 to form a core assembly 8. Preferably, there are two support members. The volume and size of the core assembly 8 are consistent with the internal space size of the box body 1. This structure facilitates assembly and improves production efficiency.

[0051] In conjunction with the movement assembly, the following box structure can be used for assembly:

[0052] Example 3

[0053] Better, such as Figure 9 As shown, the disposable fluorescent immunochromatographic test kit described above comprises a box body 1 comprising an upper shell 11 and a lower shell 12. The movement assembly 8 is placed directly within the lower shell, and the upper shell 11 is then snapped onto the outside of the lower shell 12. Specifically, the connection between the upper and lower shells can be secured using glue, double-sided tape, or the like. Accordingly, an observation window 4 and a liquid injection port 5 are provided at the top of the upper shell 11.

[0054] Example 4

[0055] Better, such as Figure 10 As shown, the disposable fluorescent immunochromatographic test kit described above comprises a hollow shell structure formed of a flexible, bendable material with an open end. The opening is extended to form an upper cover plate 13, a lower cover plate 14, a first folding plate 15, and a second folding plate 16. The movement assembly 8 is placed into the box body 1 through the opening, and then the upper, lower, first, and second folding plates are folded to seal the opening. Specifically, the joints between the upper, lower, first, and second folding plates can be secured with glue, double-sided tape, or the like.

[0056] Preferably, in the aforementioned disposable fluorescent immunochromatographic test kit, the height of the box body 1 is 9.5±5 mm. This height ensures the best visual effect and avoids uneven light spots caused by being too close, and insufficient brightness or increased power consumption and product size due to being too far away.

[0057] Preferably, the disposable fluorescent immunochromatographic detection kit is provided with fluorescent markers 33 (such as Figure 2 As shown in FIG. 1 , for example, the fluorescent marker is a circular fluorescent marker, with two on one side and one on the other side. When a user takes a photo with a mobile phone, the fluorescent marker can facilitate camera positioning.

[0058] Preferably, in the aforementioned disposable fluorescent immunochromatographic test kit, the box body can be made of black cardboard, silicone, TPU, TPC, or PVC. Preferably, the inner wall of the box body adopts a matte surface to make the light more uniform. In addition to achieving the purpose of shading, the above-mentioned materials can reduce the weight of the box body due to their lightness, making it more convenient to carry; at the same time, the buttons are wrapped inside the box body, and the elastic properties of the material are used to maintain their function. The box body does not need to have separate holes, ensuring better light-shielding properties and a simpler appearance. Preferably, the box body is sealed and packaged in a plastic bag.

[0059] The operation and working process of the disposable fluorescent immunochromatographic detection kit of the present invention are as follows:

[0060] 1. The user mixes the collected swab sample (oropharynx) with the sample preservation solution and drips the required volume into the injection hole 5 (e.g., 3 drops).

[0061] 2. Wait for a certain reaction time (such as 15 minutes).

[0062] 3. When the user presses button S1, button S1 turns on. When capacitor CB1 quickly charges to the turn-on threshold of U1's pin 4 (voltage greater than 1.6V), constant-current driver chip U1 starts operating and the LED lights up. When the user releases button S1, button S1 turns off, and the RC circuit composed of CB1 and R2 begins to discharge. When the voltage across capacitor CB1 discharges to the shutdown preset voltage value of U1's pin 4 (about 0.4V), constant-current driver chip U1 stops operating and the LED goes out.

[0063] 4. The user can observe the CT line of the reagent card after the LED lights up and within the preset delay period.

[0064] In summary, compared with the prior art, the disposable fluorescent immunochromatographic detection kit of the present invention can realize personal rapid self-service detection, is suitable for home and portable detection, has good reliability, and has a compact overall structure, reasonable layout, low production cost, and is conducive to large-scale production and processing.

[0065] The above are only preferred embodiments of the present invention. Any simple modifications and equivalent transformations of the above embodiments based on the technical solutions of the present invention fall within the scope of protection of the present invention.

Claims

1. A disposable fluorescent immunochromatographic detection kit, comprising a box body, a light source assembly and a reagent card placed in the box body, wherein the reagent card has a detection window and a sample addition hole, characterized in that: The box body is a closed shell structure made of flexible light-shielding material; the reagent card is arranged at the bottom of the box body, the light generated by the light source assembly is used to illuminate the detection window of the reagent card, and an observation window and a liquid injection hole are opened on the top of the box body, the observation window corresponds to the detection window, and the liquid injection hole corresponds to the sample addition hole; The light source assembly includes a power supply, a button, an LED unit and an LED control unit, the LED unit includes one or more LEDs, and a filter is arranged above the LED unit; The light source assembly is integrated on a control circuit board, and the control circuit board is provided with a first window and a second window, wherein the first window corresponds to an observation window, and the second window corresponds to a liquid injection hole; the LEDs are arranged along the edge of the first window; The box body includes an upper shell and a lower shell, which are relatively buckled to form a hollow shell structure with a closed circumference, the reagent card is fixed to the inner bottom of the lower shell, and the control circuit board is fixed to the inner top of the upper shell.

2. The disposable fluorescent immunochromatographic detection kit according to claim 1, characterized in that: The LED control unit comprises a constant current driving circuit, a first input end of the constant current driving circuit is connected to the output end of the power supply, and an output end of the constant current driving circuit is connected to the LED unit to maintain a constant load current of the LED unit.

3. The disposable fluorescent immunochromatographic detection kit according to claim 2, characterized in that: The LED control unit also includes an RC delay circuit, the input end of the RC delay circuit is connected to the power supply through a button, and the output end of the RC delay circuit is connected to the second input end of the constant current drive circuit. When the button is disconnected, the RC delay circuit starts to discharge, and after a preset delay period, the constant current drive circuit stops outputting current, and the LED unit is turned off.

4. The disposable fluorescent immunochromatographic detection kit according to claim 3, characterized in that: The constant current drive circuit includes a WD3139 constant current drive chip U1, an inductor L1, a freewheeling diode D20, a resistor R3, a capacitor C1 and a capacitor C2; the 6 pins of the U1 are divided into three paths, the first path is connected to the first end of the capacitor C1, the second path is connected to the first end of the inductor L1, and the third path is connected to the positive electrode of the power supply; the second end of the capacitor C1 is grounded; the 1 pin of the U1 is divided into two paths, the first path is connected to the second end of the inductor L1, the second path is connected to the positive electrode of the freewheeling diode D20, the negative electrode of the freewheeling diode D20 is divided into three paths, the first path is connected to the 5 pins of U1, the second path is connected to the first end of the capacitor C2, and the third path is connected to the positive electrode of the LED; the second end of the capacitor C2 is grounded; the 3 pins of the U1 are divided into two paths, the first path is connected to the negative electrode of the LED, and the second path is grounded through the resistor R3; the 2 pin of the U1 is grounded.

5. The disposable fluorescent immunochromatographic detection kit according to claim 1, characterized in that: The box body is formed by folding a bendable flexible material, including a front plate, a top plate, a back plate and a bottom plate connected in sequence, a first connecting plate is formed at the lower end of the bottom plate, a first side plate is formed on both sides of the top plate, a second side plate is formed on both sides of the bottom plate, a second connecting plate is formed on both sides of the front plate, and a third connecting plate is formed on both sides of the back plate; the reagent card is fixed to the inner side surface of the bottom plate, and the control circuit board is fixed to the inner side surface of the top plate; a crease is formed at the connection between the front plate, the top plate, the back plate, the bottom plate and the first connecting plate, and the first connecting plate is attached to the inner side of the front plate after being bent along the crease; a crease is formed at the connection between the front plate and the second connecting plate, the top plate and the first side plate, the back plate and the third connecting plate, and the bottom plate and the second side plate, and the first side plate is attached to the outer side of the second side plate through the second connecting plate and the third connecting plate after being bent along the crease.

6. The disposable fluorescent immunochromatographic detection kit according to claim 1 or 5, characterized in that: At least one support member is arranged between the control circuit board and the reagent card, and the bottom end surface of the support member is fixed to the end surface of the reagent card and / or the top end surface of the support member is fixed to the end surface of the control circuit board.

7. The disposable fluorescent immunochromatographic detection kit according to claim 1, characterized in that: The control circuit board and the reagent card are fixedly connected via a support member to form a movement assembly.

8. The disposable fluorescent immunochromatographic detection kit according to claim 7, characterized in that: The box body comprises an upper shell and a lower shell, the movement assembly is placed in the lower shell, and the upper shell is buckled on the outer side of the lower shell.

9. The disposable fluorescent immunochromatographic detection kit according to claim 7, characterized in that: The box body is formed of a bendable flexible material to form a hollow shell structure with an opening at one end. The opening extends to form an upper cover plate, a lower cover plate, a first folding plate, and a second folding plate. The movement is always placed in the box body through the opening. The upper cover plate, the lower cover plate, the first folding plate, and the second folding plate are folded to close the opening.

10. The disposable fluorescent immunochromatographic detection kit according to claim 1, characterized in that: The height of the box body is 9.5±5 mm.

11. The disposable fluorescent immunochromatographic detection kit according to claim 1, characterized in that: The detection window of the reagent card is provided with fluorescent markers on both sides, with two on one side and one on the other side.

12. The disposable fluorescent immunochromatographic detection kit according to any one of claims 1 to 5 and claims 7 to 11, characterized in that: The box body material is black cardboard, silica gel, TPU, TPC or PVC.

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