A Helicobacter pylori whole protein chip and its preparation method and application

Abstract

The present invention discloses a Helicobacter pylori whole protein chip and a preparation method and application thereof, wherein the Helicobacter pylori whole protein chip comprises a substrate and 1631 kinds of Helicobacter pylori proteins connected thereto. The Helicobacter pylori whole protein chip prepared by the present invention can be used to screen Helicobacter pylori proteins that interact with small molecule compounds, and has the characteristics of global and high-throughput screening, simplifies the research design and operation process, and significantly improves the research efficiency.

Description

Technical Field

[0001] The present invention relates to the field of biomedicine technology, and specifically to a Helicobacter pylori whole protein chip and a preparation method and application thereof, and in particular to a Helicobacter pylori whole protein chip and an application thereof in screening small molecule compounds and Helicobacter pylori interacting proteins. Background Art

[0002] Helicobacter pylori is a spiral-shaped, slightly anaerobic bacterium commonly found in the human gastric mucosa. It was first isolated by Marshal and Warren in 1983 from gastric mucosal biopsy tissue of patients with chronic gastritis. Helicobacter pylori is highly infectious and can be transmitted from person to person through oral-oral and fecal-oral routes, causing digestive tract diseases such as chronic gastritis, peptic ulcer, gastric atrophy, intestinal gastric cancer, and affecting the occurrence and development of extragastric diseases such as cardiovascular, neurological, and immune system diseases. As of 2015, approximately 4.4 billion people worldwide were infected with Helicobacter pylori, of which approximately 770 million were infected in my country. As of October 27, 2017, the World Health Organization's International Agency for Research on Cancer classified Helicobacter pylori (infection) as a Class 1 carcinogen. my country also recognized Helicobacter pylori infection as an infectious disease in the 2019 consensus.

[0003] Helicobacter pylori infection is serious, but the prevention and treatment effect is not ideal. The main reason is that the strain has serious drug resistance and the resistance mechanism has not been fully clarified. Helicobacter pylori resistance can be divided into primary resistance and secondary resistance. The latter is due to the strain acquiring drug resistance genes, thus changing from the original sensitive bacteria to resistant bacteria. Tests have found that the resistance of Helicobacter pylori is mainly secondary resistance. According to statistics, the resistance rates of Helicobacter pylori to clarithromycin, metronidazole and levofloxacin (fluoroquinolones) in my country are 20% to 50%, 40% to 70%, and 20% to 50%, respectively. Helicobacter pylori can develop double, triple or more resistance to these antibacterial drugs, and there are certain regional differences in the resistance rates of the same strain. Studies have shown that the most common multi-drug resistance of Helicobacter pylori in clinical practice is triple resistance to clarithromycin, metronidazole and fluoroquinolones. In addition, to date, no single therapy can achieve an eradication rate of Helicobacter pylori greater than 80%. And with the increase in the drug resistance rate of Helicobacter pylori, the traditional proton pump inhibitor (PPI)-based triple therapy (PPI plus two antibiotics) that has been widely used in the past 20 years to eradicate Helicobacter pylori has an eradication rate of Helicobacter pylori that is lower than or far lower than 80%. Against this background, quadruple therapy (bismuth + PPI + tetracycline + metronidazole) has been developed. Although quadruple therapy has achieved good therapeutic effects, it is still necessary to pay attention to dosage, course of treatment and contraindications during clinical application. Due to the high drug resistance of Helicobacter pylori, even if all the regimens recommended in the global treatment guidelines are applied to the first-line treatment, there are still about 10-30% of patients who cannot achieve the eradication of Helicobacter pylori, and even cause treatment difficulties for patients who cannot be cured by continuous drug treatment regimens. In view of the serious problem of Helicobacter pylori resistance, and the lack of major breakthroughs in the research and development of related therapeutic drugs, there is an urgent need to find new drug targets.

[0004] In recent years, some biological small molecules have been found to be related to bacterial drug resistance and thus have been used as new targets for antibiotic research and development. For example, NO can help bacteria acquire broad-spectrum drug resistance through a variety of mechanisms; H2S can resist oxidative stress damage caused by antibiotics by activating catalase and superoxide dismutase, thereby enabling bacteria to acquire drug resistance; indole can activate genes related to drug efflux pumps to achieve antibiotic tolerance; small molecules such as c-di-GMP (cyclic diguanylate) and ppGpp (tetraphosphate guanosine) can regulate the expression of related genes, such as extracellular polysaccharides (EPSs), thereby producing a physical barrier and improving tolerance to antibiotics. However, the specific molecular mechanisms of drug resistance produced by these signal small molecules in Helicobacter pylori have not been reported, and there is no kit specifically for screening proteins interacting with small molecules in Helicobacter pylori. Summary of the invention

[0005] In view of the deficiencies of the prior art, the purpose of the present invention is to provide a Helicobacter pylori whole protein chip and its preparation method and application. Based on the Helicobacter pylori whole protein chip prepared by the present invention, global small molecule interacting proteins are sought and functional studies are conducted on them, thereby more clearly and accurately understanding the role of small molecules in Helicobacter pylori. The purpose of the present invention is achieved through the following technical solutions:

[0006] In a first aspect, the present invention provides a Helicobacter pylori whole protein chip, comprising a substrate and 1631 Helicobacter pylori proteins connected thereto.

[0007] Preferably, the 1631 Helicobacter pylori proteins are from Helicobacter pylori 26695 and Helicobacter pylori J99 strains.

[0008] Preferably, the substrate is an optical grade epoxy substrate.

[0009] In a second aspect, the present invention provides a method for preparing a Helicobacter pylori whole protein chip, comprising the following steps:

[0010] A. preparing recombinant expression vectors containing nucleic acid sequences encoding 1631 Helicobacter pylori proteins, and transforming them into Escherichia coli;

[0011] B. High-throughput preparation and purification of various Helicobacter pylori proteins;

[0012] C. The purified Helicobacter pylori proteins are prepared into sample solutions and added to the corresponding well plate positions, and then spotted on the substrate by a spotter to obtain a full protein chip.

[0013] Preferably, in step A, the nucleic acid sequences encoding 85 Helicobacter pylori proteins among the 1631 Helicobacter pylori proteins are shown as SEQ ID NOs. 1 to 85.

[0014] Preferably, in step B, the high-throughput preparation step is: after culturing the E. coli obtained in step A in LB medium, IPTG is added to the obtained bacterial solution for induction culture, the supernatant is removed by centrifugation, and the obtained strain is stored for future use.

[0015] Preferably, in step B, the purification step is:

[0016] S1. After the strain prepared by high-throughput is frozen and thawed, a mixture formed by mixing Lysis buffer and pretreated Ni-NTA is added, and the mixture is mixed and incubated;

[0017] S2. Eluting the incubation solution obtained after incubation to obtain purified Helicobacter pylori proteins.

[0018] In the high-throughput preparation process of the present invention, Escherichia coli containing the recombinant expression vectors of various Helicobacter pylori proteins is directly used for subsequent culture and purification.

[0019] In a third aspect, the present invention provides a kit containing the aforementioned Helicobacter pylori whole protein chip.

[0020] In a fourth aspect, the present invention provides a use of the aforementioned Helicobacter pylori whole protein chip or the aforementioned kit in screening Helicobacter pylori proteins that interact with small molecule compounds.

[0021] Preferably, the small molecule compounds include nucleotide molecules, antibiotics, and metabolites.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] 1. Exogenous expression of Helicobacter pylori protein usually uses Escherichia coli as a host, but it is a huge project to realize the cloning and expression of 1631 proteins in the whole protein group. In addition, Helicobacter pylori is often faced with the problem of low expression amount and success rate when expressed in Escherichia coli. In order to solve the above problems, the present invention utilizes sequence alignment, codon optimization, gene synthesis, especially for 85 proteins that are difficult to express and purify, and by adopting Escherichia coli to carry out high-throughput preparation and purification of protein, each expression strain containing 1631 proteins of Helicobacter pylori with a high success rate is obtained.

[0024] 2. The Helicobacter pylori whole protein chip prepared by the present invention can be used to screen Helicobacter pylori proteins that interact with small molecule compounds, and has global, high-throughput screening characteristics, which simplifies the research design and operation process and significantly improves the research efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Other features, objects and advantages of the present invention will become more apparent from the detailed description of non-limiting embodiments made with reference to the following drawings:

[0026] Figure 1 The quality inspection results of some proteins obtained in Example 1;

[0027] Figure 2 The quality control results of the whole protein chip prepared in Example 1;

[0028] Figure 3 The results of the interaction between c-di-GMP and Helicobacter pylori protein in Example 2;

[0029] Figure 4 is a list of Helicobacter pylori proteins that interact with c-di-GMP obtained by screening in Example 2;

[0030] Figure 5 This is the result of verifying the interaction between c-di-GMP and protein P56013 by BLI in Example 2. DETAILED DESCRIPTION

[0031] The present invention is described in detail below in conjunction with specific embodiments. The following embodiments will help those skilled in the art to further understand the present invention, but are not intended to limit the present invention in any form. It should be noted that, for those of ordinary skill in the art, several variations and improvements may be made without departing from the concept of the present invention. These all belong to the protection scope of the present invention.

[0032] The experimental methods used in the following examples are conventional methods unless otherwise specified. The materials, reagents, etc. used in the following examples are all commercially available unless otherwise specified.

[0033] Example 1

[0034] This embodiment provides a Helicobacter pylori protein chip, including 1631 Helicobacter pylori proteins and a chip substrate. The 1631 Helicobacter pylori proteins are shown in Table 1, and the chip substrate is an optical grade epoxy substrate purchased from Beijing Bio Biochip Co., Ltd.

[0035] The method for preparing the Helicobacter pylori whole protein chip comprises the following steps:

[0036] (I) Acquisition of Helicobacter pylori genes and preparation of expression vectors

[0037] 1. Using the Uniprot website, the full protein groups of Helicobacter pylori strains 26695 and J99 were downloaded, which were 1515 and 1524 respectively. Multiple sequence alignment was performed using BLAST, and duplicate sequences were removed according to a threshold of 90%. Finally, 1631 protein sequences were obtained as shown in Table 1.

[0038] 2. The 1631 protein sequences were further analyzed, and codon optimization was used to avoid GC content, highly repeated sequences, complex secondary structures, longer fragment lengths, and other issues. The E. coli codon usage table was used to convert the sequences and convert the above protein sequences into nucleic acid sequences. Among them, the proteins numbered NO.1000-1084 in Table 1 need to be converted into nucleic acid sequences corresponding to SEQ ID NO.1-85, so as to obtain high protein expression; if the nucleic acid sequence is other nucleic acid sequences encoding the corresponding protein, it will result in low protein expression.

[0039] 3. Use oligo chemical synthesis to synthesize small fragment primers of 40 to 200 nt with overlapping regions by conventional methods.

[0040] 4. Through seamless cloning and assembly technology, the nucleic acid fragments of each protein are spliced ​​into full length and inserted into the pET28a vector to form a recombinant expression vector containing each protein, and the final quality inspection of the finished product is carried out through sequencing verification.

[0041] 5. The recombinant expression vector was transferred into E. coli BL21 by CaCl2 chemical transformation method to form E. coli containing the recombinant expression vectors of each protein.

[0042] Table 1

[0043]

[0044]

[0045]

[0046]

[0047]

[0048]

[0049]

[0050]

[0051]

[0052]

[0053]

[0054]

[0055] (II) High-throughput preparation of Helicobacter pylori proteins

[0056] 1. Strain Cultivation and Induced Expression

[0057] 1.1 Bacteria selection

[0058] Use an electric gun to dispense 1 mL of LB medium (with kanamycin added to a final concentration of 50 μg / mL) into each well of a 96-deep-well plate. The formula of LB medium is shown in Table 2.

[0059] The E. coli containing the recombinant expression vectors of each protein prepared in step (i) was taken out from -80°C, and a little ice was added to the culture medium in each well using a 10 μL pipette tip. The culture was carried out overnight at 37°C and 200 rpm (about 16 hours, OD was about 7-9).

[0060] 1.2 Transfer

[0061] Aliquot the culture medium: Prepare 3000 mL of LB (Kana+) culture medium, and use a 5 mL pipette to dispense 5 mL of LB (Kana+) culture medium (with kanamycin added at a final concentration of 50 μg / mL) into each well of a 12-well plate. LB (Kana+) is LB culture medium with kanamycin added.

[0062] Transfer bacteria: Use a 12-pass pipette to transfer 50 μL of the overnight culture solution into the newly packaged culture medium mentioned above, and culture at 37°C, 90 rpm until OD = 0.5 (2h) for subsequent induction.

[0063] 1.3 Induction

[0064] Protein induction: Dilute IPTG to a concentration of 20 mM with 40 mL of LB medium containing Kana+. Use a 12-channel pipette to draw 50 μL of the diluted IPTG and add it to the bacterial solution cultured in step 1.2 (so that the final concentration of IPTG is 0.2 mM), and induce the culture at 23°C and 90 rpm for 8 to 10 hours.

[0065] Note: All the above operations are performed in a sterile environment.

[0066] 1.4 Harvesting bacteria

[0067] Centrifuge the 12-well plate after induction culture at 3900rpm for 10 minutes and carefully discard the supernatant. Resuspend the bacteria with a spray gun and transfer the resuspension to a 96-well plate. After all the transfers are completed, centrifuge the 96-well plate at 3900rpm for 10 minutes and discard the supernatant (shake slightly). Then seal the top of the 96-well plate with collected bacteria with a lid + transparent sealing film and store it at -80℃.

[0068] 2 Protein purification

[0069] 2.1 Freezing and thawing of strains

[0070] The 96-well plate containing the strain was taken out from -80℃ and placed at room temperature. The strain was allowed to thaw (it takes about 10 minutes and can be shaken appropriately to disperse the strain). It was then frozen at -80℃ for 10 minutes and placed at room temperature for 10 minutes. This process was repeated twice (the strain was frozen and thawed three times).

[0071] 2.2 Column pretreatment

[0072] Ni-NTA pretreatment: Take 4 mL Ni-NTA, wash twice with 3 column volumes of PBS (precooled), wash twice with 2 column volumes of H-Base buffer (PH 8.0), and finally remove the supernatant and keep Ni-NTA for use (centrifugation at 2000 rpm, 1 min, 4°C during washing). The formula of H-Base buffer used is shown in Table 3.

[0073] 2.3 Configuring Lysis Buffer

[0074] The configuration of Lysis buffer is as follows: CelLytic B (1X), lysozyme (1X), nuclease (50 units / mL), protease inhibitor (1:100), PMSF (1 mM), and pre-treated Ni-NTA are added to H-Base buffer (PH 8.0).

[0075] 2.4 Bacterial lysis and incubation

[0076] Mix the Lysis buffer with the Ni-NTA pretreated in step 2.2 above, and add 200 μL to each well of the 96-well plate containing the strain using a 12-well pipette. Mix well with a pipette (mix thoroughly before adding, and cut off a portion of the front end of the pipette tip with scissors to prevent Ni-NTA from clogging the pipette tip). Incubate at 4°C for 1 hour. (Move the shaker to the refrigerator, set the speed to 250 rpm, and observe whether there is precipitation)

[0077] 2.5 Protein elution

[0078] 2.5.1 Place the 96-well filter plate on a clean 96-well plate, pipette and mix the incubation solution (which may contain beads) obtained in step 2.4 above, transfer it to the 96-well filter plate, centrifuge (stop when the speed reaches 2000 rpm), collect the flow-through, and store it at -80°C.

[0079] 2.5.2 Place the 96-well filter plate on another 96-well plate to collect waste liquid, wash the 96-well filter plate three times with 200 μL Wash Buffer (wash with H-Wash Buffer shown in Table 4), and during the third wash, centrifuge at 2000 rpm for 1 min to remove the Wash Buffer.

[0080] 2.5.3 Then place the washed 96-well filter plate on a 96-well PCR plate, add 50 μL Elute Buffer (wash with H-Elute Buffer shown in Table 5), incubate at 20°C on a horizontal shaker at 160 rpm for 15 min, then collect the eluate at 2000 rpm for 1 min, and perform elution 4 times in a row to obtain the purified proteins.

[0081] Table 2 LB medium ( / L)

[0082]

[0083]

[0084] Table 3 H-Base buffer (PH 8.0)

[0085]

[0086] Table 4 H-Wash Buffer (PH 8.0)

[0087]

[0088] Table 5 H-Elute buffer (PH 7.5)

[0089]

[0090] 3. Protein test

[0091] 3.1 Glue preparation

[0092] Prepare 12% SDS-PAGE gel as shown in Table 6-7, 1.0 mm, 15 pores.

[0093] 3.2 Sample preparation

[0094] Take 8 μL of the purified proteins above, add 2 μL of 6x Loading buffer, and cook the samples at 98°C for 5 min.

[0095] 3.3 Glue running

[0096] Add 2 μL marker and 10 μL sample prepared in step 3.2 to each well in step 3.1. The electrophoresis conditions are as follows:

[0097] S1: 80V, 30min; S2: 130V, 45min.

[0098] 3.4 Dyeing

[0099] 3.4.1 Staining: Add an appropriate amount of staining solution (prepared as shown in Table 8) to each well, heat for 15 seconds to raise the temperature to about 50°C, and then transfer to a shaker for staining for 20 minutes.

[0100] 3.4.2 First decolorization: Transfer the stained gel into the decolorization solution (prepared as shown in Table 9) and decolorize on a shaker.

[0101] 3.4.3 Second decolorization: Transfer the gel after the first decolorization into the decolorization solution (prepared as shown in Table 9), heat to about 50°C, and decolorize on a shaker for 30 minutes.

[0102] 3.4.4 Scanning: Scan the gel after the second destaining process with a scanner and save the image. Some of the results are as follows: Figure 1 As shown, the sizes of the proteins are correct. Figure 1 The statistical results showed that 1420 proteins (87%) were successfully purified and the concentration was higher than 0.1 mg / mL; while the remaining 211 proteins had a low concentration after purification, between 0.01-0.1 mg / mL, but still met the standards for protein chip preparation.

[0103] In the early stage of this embodiment, the method in patent document CN103884847A was also investigated for induction culture, that is, the recombinant expression vector in Escherichia coli was transformed into a cerevisiae strain for induction culture and subsequent purification. The results showed that the purification efficiency of this method was low, and the protein concentration was low above 85%.

[0104] Table 6 12% SDS-PAGE lower gel (40 mL)

[0105]

[0106] Table 7 SDS-PAGE upper gel (16mL)

[0107]

[0108] Table 8 Test dye solution ( / L)

[0109]

[0110] Table 9 Staining solution ( / L)

[0111]

[0112] (III) Preparation of whole protein chip

[0113] 1. Preparation of control samples: 9 positive control samples required for protein chip were prepared. The specific volumes of each component are shown in Table 10. Each sample was used as a mother solution and mixed with PBS and glycerol to obtain a control sample with a final dilution concentration.

[0114] Table 10

[0115]

[0116] 2.384-well plate spotting

[0117] 2.1 First arrange the order of the required samples on the Excel table, and then use the spotting instrument software Command Centre TM The samples were processed by Marathon v2.2 to obtain an array table of samples distributed on a 384-well plate.

[0118] 2.2 Take 15 μL of each of the 9 prepared positive control samples and add them to the corresponding wells according to the array table; dispense 1631 purified Helicobacter pylori proteins to the corresponding wells on the 384-well plate, 15 μL each. Seal the plate and store at 4°C.

[0119] 3. Using a spotting instrument to prepare slides

[0120] Place the 384-well plate and substrate on the spotter, set 6 replicates and 14 blocks, adjust the layout parameters, spot the whole protein chip and store it at -80℃.

[0121] 4. Chip storage

[0122] The spotted whole protein chip was taken out and placed in a chip box and stored at -80°C.

[0123] (IV) Quality control of whole protein chip

[0124] 1. Antibody coating (serum)

[0125] 1.1 Take out the whole protein chip from -80℃, freeze it at -20℃ for 20min, then take it out and place it at 4℃ for 20min, and finally thaw it at room temperature.

[0126] 1.2 The whole protein chip was pre-blocked with BSA (blocking solution preparation is shown in Table 11) and shaken on a uniaxial shaker for 2 h.

[0127] Table 11 Blocking solution (PBS solution; pH 7.4)

[0128]

[0129]

[0130] 1.3 Prepare antibody coating solution: dilute 1 μL serum with 199 μL TBST, mix well and add to the corresponding area of ​​the chip. For control, dilute 0.2 μL BSA with 199 μL TBST, mix well and add to the corresponding area of ​​the chip.

[0131] 1.4 Place the chip fence on a unidirectional shaker and stir at less than 20 rpm / min for 2 hours.

[0132] 2. PBST Washing

[0133] 2.1 Discard the serum coating, wash each module three times with PBST solution (prepared as shown in Table 12), and remove the fence.

[0134] 2.2 Add an appropriate amount of 1×PBST to the chip cleaning tank and shake manually to clean twice.

[0135] 2.3 Continue to add 1×PBST to the chip cleaning tank, place it on an axial shaker and wash for 10 minutes, repeat 3 times.

[0136] 3. Secondary Antibody Incubation

[0137] 3.1 Pipette 3 mL 1×PBST into a 6 mL Eppendorf tube, add 3 μL of each of the three secondary antibodies (Cy3-conjugated AffiniPure Goat Anti-Human IgG; Alexa Fluor 488-conjugated AffiniPure Rabbit Anti-Human Serum IgA; Alexa Fluor 647-conjugated AffiniPure Donkey Anti-Human IgM), mix well and place at 4°C for later use.

[0138] 3.2 After washing the chip, pour it into the Eppendorf tube containing the secondary antibody mixture and incubate for 1 hour.

[0139] 4. Washing

[0140] 4.1 Add 1× PBST to the washing tank and wash on an axial shaker for 10 minutes. Repeat 3 times.

[0141] 4.2 Rinse with clean water for the last time and shake on a shaker for 10 minutes.

[0142] 4.3 Place the cleaned chip on the chip rack, centrifuge at 1000 rpm / min for 1 min, and spin dry.

[0143] 5. Scan

[0144] 5.1 Set the scanner parameters: Power = 95, PMT = 550 (CY3 red channel)

[0145] Power = 100, PMT = 550 (CY5 green channel)

[0146] 5.2 Pre-scan the chip, initially determine the fine scanning area and scanning parameters, and obtain the binding information in advance.

[0147] 5.3 Scan the chip carefully.

[0148] 6. Scan Results

[0149] The data were read using a Genepix scanner at 532 nm. Figure 2 As shown, the quality control showed that the signal-to-noise ratio of the fluorescence intensity of each protein was significant and could be used for downstream proteomics chip applications.

[0150] Table 12 PBST solution (pH 7.4)

[0151]

[0152] Example 2 Interaction between whole protein chip and small molecule compound c-di-GMP

[0153] c-di-GMP (cyclic diguanylate) is an important second messenger molecule widely present in bacteria. It can regulate various biological functions of bacteria at the transcription, translation and post-translation levels, such as regulating the bacterial cell cycle, biofilm formation, cytotoxicity and diffusion, and motility.

[0154] After Helicobacter pylori infects a host, it can evade or suppress the host's immunity through its own mutation or physiological changes, thereby improving its ability to survive. Although current research has made some progress, further exploration is still needed in view of the complex biological characteristics and pathogenic mechanism of Helicobacter pylori. Cyclic diguanylate (c-di-GMP) is a second messenger widely present in bacteria. It not only has a wide range of regulatory functions, such as bacterial movement and transcriptional regulation, but also can be secreted by bacteria to activate the human body's inherent immune pathway, playing an important role in pathogen-host interaction. However, there are currently few studies on c-di-GMP in Helicobacter pylori, and it is not clear how c-di-GMP is regulated in Helicobacter pylori. Based on this, the Helicobacter pylori full protein chip prepared by the present invention can be used to find global c-di-GMP interacting proteins, conduct functional studies on them, and thus more clearly and accurately understand the role of c-di-GMP in Helicobacter pylori.

[0155] The specific steps of searching for global c-di-GMP interacting proteins using the Helicobacter pylori whole protein chip prepared in Example 1 are as follows:

[0156] 1. Take out the Helicobacter pylori proteome chip from -80°C.

[0157] 2. Closure

[0158] Weigh 0.6 g BSA, add 20 mL PBST, prepare a chip blocking solution with 3% BSA, add 10 mL to each whole protein chip, place on a shaker, 4°C, 60 rpm, and block for 2 h.

[0159] 3. Use PBST to prepare 3 mL, 10 μM Biotin-c-di-GMP as the reaction solution and 3 mL, 10 μM Biotin as the control solution.

[0160] 4. Add the reaction solution and control solution of step 3 into different chip reaction tanks respectively, react at 4°C, 60rpm, and react for 12h.

[0161] 5. Discard the reaction solution obtained after step 4 and elute the chip with 10 mL PBST at room temperature for 3 times, each time for 5 min.

[0162] 6. Add 3 mL of Cy5-Streptavidin diluted 1:1000 in PBST and react at room temperature and 60 rpm for 1 hour.

[0163] 7. Discard the reaction solution obtained after the reaction in step 6, and elute the chip with 10 mL PBST at room temperature for 3 times, each time for 5 minutes.

[0164] 8. Finally, elute once with ddH2O at room temperature for 5 min. Dry the water using a chip centrifuge.

[0165] 9. Place the chip in the chip scanner, read the chip signal using the 635 fluorescence channel, and generate GPR raw data.

[0166] 10. Protein chip data analysis The data analysis system of the protein chip database was used (www.proteinmicroarray.cn / index.pHelicobacter pylori / analysis-toolkits).

[0167] 10. Select SNR ≥ 1.5 as a positive signal and screen to obtain a candidate list of c-di-GMP interacting proteins.

[0168] 12. Upload the candidate proteins obtained by screening to the Uniprot website for protein name annotation.

[0169] The results are as follows Figure 3 and Figure 4 As shown, the SNR of proteins O25693, O25769, O25061, O25343, P56013, O25531, P56133, and O25705 were >1.5, and the interaction between c-di-GMP and protein P56013 was verified by BLI (the results are shown in Figure 5 This indicates that these proteins screened through the whole protein chip interact with the small molecule compound c-di-GMP.

[0170] Therefore, based on the above verification results, it can be seen that the Helicobacter pylori whole protein chip prepared by the present invention can be used to screen Helicobacter pylori proteins that interact with cyclic diguanylate or to construct a database of Helicobacter pylori proteins that interact with cyclic diguanylate.

[0171] It should be noted that, based on the verification method and results of Example 2, the whole protein chip prepared by the present invention can also be used by existing conventional methods to screen Helicobacter pylori proteins that interact with other small molecule compounds (such as nucleotides, antibiotics, metabolites, etc., but not limited to these), and used to construct a database of Helicobacter pylori proteins that interact with other small molecule compounds.

[0172] The present invention has many specific application paths, and the above is only a preferred embodiment of the present invention. It should be pointed out that the above embodiments are only used to illustrate the present invention, and are not used to limit the protection scope of the present invention. For those of ordinary skill in the art, several improvements can be made without departing from the principle of the present invention, and these improvements should also be regarded as the protection scope of the present invention. Sequence Listing <110> Fujian Medical University <120> A Helicobacter pylori whole protein chip and its preparation method and application <130> PO5222142 <160> 85 <170> SIPOSequenceListing 1.0 <210> 1 <211> 978 <212> DNA <213> Artificial Sequence <400> 1 atgttaaaaa catatcacat agcactagct tgcgttatcc tggccgttgt tgtactgttg 60 ttcggcggtg aaagcctttc actggaggag tggcaagagg tgtgtctgaa tgttaaaaac 120 cattttctgc acaacgagga actgagcagc ctttcgatca tcattctgga aattcgtttg 180 ccgcgtgtta ttctcgcgct gttggtcgga gcgtccttat cgggcagcgg cgtggtgatg 240 cagactatct ttcgcaaccc gttggttgat ccgtttctgt tgggtatcag ctctggcgct 300 atgctgggcg ttgccatggc catcgctgtg gtcgaaagca atatcgcgat tctggcgttt 360 ttcggtgcga ttctggcgag cctggcggtg ctggctatga accgcgttct gggtaatagc 420 gtcctgtctc tggtcctgag tggtgtggtg cttagcgcgt tcctgagcgc tctggcgggt 480 gcgatcaaat tcttcgtgat cccgcagaag gcacaagcaa tcgtggtttg gttgctgggt 540 agcttgtctc tatccagcta taggactgt cttatcgcct ttatcggct gtcgttaggc 600 tttatcccac tgttcctgct gcgttggcgt attaacctgc tgtctttgtc cgacgcacaa 660 agcctttccc tcggcattaa cccggtttta ttaagaagcc tttgcctggt gtgcgtgtct 720 gttgctagcg cgctggcggt ttccgtgagc ggcaccattg gctggattgg cctggtgatc 780 ccgcacgtag cacgtctgtt ctttggtgcg aatctgcaga agctgctgtt gtcctcattg 840 ctgatgggtg cgttcttcct cttgctggcg gatgttgtgg caaaaaccat taccccgtac 900 gacctgcccg ttggtattgc cacgagcgtg ctgggtgctc cgtttttcct gtggctgctc 960 ttccgcaccc gtggtgtc 978 <210> 2 <211> 978 <212> DNA <213> Artificial Sequence <400> 2 atggctaaac acgatctagt aggatcagtt ttatgggatg cttactccaa agaagtgcaa 60 agacgtatgg acaacccgac tcacctgggt gttatcaccg aagagcaggc taaggcgaag 120 aacgccaagc tgatcgtggc tgattacggc gcggaagcgt gcggcgacgc ggttcgtctg 180 tactggctgg ttgacgagag caccgaccgt atcgtggacg cgaagtttaa atcattcggc 240 tgcggtacgg ctattgcaag ctcggacatg atggtagagc tgtgtctgaa taaacgcgtg 300 caggatgcgg ttaaaatcac gaacttggac gtggagcgcg gtctgcgtga tgacccggac 360 accccggcag tgccgggtca gaaaatgcat tgctctgtga tggcatatga cgtcatcaaa 420 aaggccgcgg gcatgtatct gggtaaaaac gcggaagatt ttgaagagga gatcattgtt 480 tgcgagtgcg cgcgtgtcag cctgggcacg attaaggagg tgatcaagtt gaatgatttg 540 aagtccgtgg aggaaattac caactacacc aaagcgggtg cgttctgcaa atcttgtgtt 600 cgcccaggcg gtcacgaaaa gcgcgactac tatttagttg acatcctcaa ggaggttcgc 660 gaagagatgg aagctgaaaa gctgaaagcg accgctaata aatcccagag cggtgaactg 720 gcatttcgtg agatgaccat ggttcaaaag attaaagcgg tggataaagt catcgacgag 780 aacattcgtc cgatgcttat gatggatggt ggcgatctgg aaatcttgga catcaaggag 840 agcgatgatt acattgatgt ttatatccgt tatatgggag catgtgatgg ctgcatgagc 900 gcaaccaccg gtactttgtt cgccattgaa aatgccctgc aagaactgct ggaccgtagc 960 attcgtgtcc tgccgatt 978 <210> 3 <211> 978 <212> DNA <213> Artificial Sequence <400> 3 atgcacaaaa agagggtatt ttcaggaata caaccgaccg gtcagattca cctgggcaac 60 tatttgggcg caatcaaaca ttgggtcgag atgcaagatg agtatgagaa cttgttttgt 120 gtggtgaaca gccacgctat caccctgccg atcgatccgg ccttcttgaa gtcccagtcc 180 tacgagttag ttaaactgct gttggcgtgc ggcattgatc cgaaacaatc tggtctgttt 240 atccagagcg aagtggatga acaccctgca ctcgcgtggc tgctgaactg ccaagtgagc 300 atgggtgaga tgcagcgtat gacccagttt aaggacaaat ctctgaaaaa tccgaagagc 360 gttaatgtag gcttgttcaa ctacccaatc ttgatggcga gcgacatcct gctgtaccag 420 agtgacctgg taccggtggg cgaagatcag aaacagcatc tggaacttac ccgtaatatt 480 gcggagaagt tcaaccgtga ttcggcaac tgctttaagg tgccggaacc gctgattgct 540 aaagtgggcg ctcgtgttat ggggttggac gacccgaagg ttaaaatgag caaatcgcac 600 cagggtgcta atcatgcgat cttcctgctg gacgagccag atattattgt caagaagatc 660 aagaaggcgg caactgacag catgggtgtt atcgcctttg atgaaaaacg tgaaggtatt 720 ttcaacctat tgaacattta tatgctgtta tccaacgaat ccccggaaaa catcgaagag 780 cgctttaaga ataaaggtta tggtgatttc aaaaaggagc tcgcggaagt tatgatccaa 840 gcactgaaac cgattcaaga acgctacaaa gagatcagcg acgacgaggt taaggcgatt 900 ctgaatggcg gtgcggagaa ggcccgtccg cttgcccgcg cgacgtacca aaaagctaaa 960 gagctgatgg gtctgatc 978 <210> 4 <211> 981 <212> DNA <213> Artificial Sequence <400> 4 atgttaaaag gactaaagaa agcatttaaa gagcgtttct gcagccaagt ctacatcagc 60 tttaatgttg accacaatct gttgtcgacc caggttattc gtattaaaaa cgatcgtatc 120 aaggagaagt tctttaagac ctttgagacg aaagtggaaa ccaagaacgg cgaagtgccg 180 attcaagccc tgaaaatcgc ccgtacctat tcccaaaagt acccgtacac ctacttctct 240 gcaatgagca aggcgaaaga agtcctgtgt gaaaagcagg ctttcgaaca aatcaaacaa 300 gagaaccagg attatcatgc gtgcgaggtg aatcaaaaat actgcgttta cgtggaaagc 360 aaagattttc tgaaggactt caagcgcttc aagattcagg atgtcgattt tctgttctcg 420 ccgtttagcc tgatttatga tttcgtgcgt gataacttag agaacaaacc gctgctttat 480 ctgctgcttg agcgcagccg tttttatttc ctaatcgcag ataaaaaaga aatctttttg 540 gcgaaaagcg tttttttgga ggaacagccg gaagagttca ttgagtctaa ggaggaagac 600 ttcatgggta tggataacga agcggtggac ctgttcctga gcgaaatcca ggaggacatc 660 gactccttgg aggaggcgat tggcctggac tccagcaagg acaacagcga aaaaatcact 720 gaagatgcat attctctcat cgagggcatg accaatattc cattgatagc ggatgttctg 780 caagaaggtc tgcgtggtgt ttaccactca agagaaattg actttgttga gaaagttgtg 840 gtgctggaca gctgtcagat ccaccagaag gctctgatgc atctgcagga gacgctgatg 900 attgaggtag accgtctgga cttctccttg gttgagcgct tgaatatcct ggctcgcatg 960 gaaaacgaga agcatgcgtt t 981 <210> 5 <211> 981 <212> DNA <213> Artificial Sequence <400> 5 atgcaaaaag tatttatagc tctaacagat cacaaacgtc tggacgagtt cctggcgaaa 60 gagctgcaaa tcagcaaaaa tcaggttttg aacctgatca aagagggcct ggttttctgc 120 cagaaaaaag aggtgaaaaa gggtggcttg gcgctgaaag agggcgatga aatcaccctg 180 ttgaccccga aaatcacgcc gaaaccgctg aagaaagaat tggacctgga aatcgaagtg 240 atttttgaag atgaagatct gttggtactg aacaaaccac cgaatttggt tgttcacaaa 300 gcactttccg tgaaggagcc gacgctggtc gattggctgg agttcaagaa ctacgaattg 360 tctaacctgg gtcttaagga gcgctacggc atcgtgcacc gtctggacaa agacaccagc 420 ggtggtattg tcattgccaa gaacaacttt acccacgttc acctctcaga acagctgaag 480 accaaaatga tgggtagata ctacatcgcg ctgttatcga ccccgctgaa ggaggaaaag 540 atgagcgttg aatgttattt gacccgtaat cctaataacc gcctgaagat gattgctctc 600 aaggctgtga agaaagagaa gagccgttac agcaagtctg agttcatctc cctgctgacg 660 agccaaaaca acttgaacct gattggcgcg aaactgttta ctggtcgcac ccatcagatt 720 cgcgcacatc tggagtacct gaatcgtcat attatcggcg acaacctgta tggtctgaat 780 ggtgcccttc cgaaggaaga gatccgtatt atgctgcacg cttatctgat tgagttcaag 840 catccgcgta gcgaacaaaa actgcgtttc aaggtgccgc tcttgaagga catgctggag 900 tatttgaaga aagtttttga taaagagaac ctagacgaag tccttgatga ggaaaagatc 960 ctgcatgcgt ttatcgcgaa a 981 <210> 6 <211> 981 <212> DNA <213> Artificial Sequence <400> 6 atgtcactaa aagtatttga ttatgaagac gtacagctga ttccgaataa atgcattgtg 60 aacagccgtt ccgagtgcga taccaccgtt attctgggta aacacgcctt taaaatgccg 120 atcgtgccgg caaatatgca aaccatcatt aacgaaagca tcgcggagtt cctggccgaa 180 aacggatatt tctatatcat gcaccgtttc gatggtgcag cgcgtattcc gttcgtgaag 240 aagatgaaaa agcgccagtg gatttcttcc attagtgttg gtgttaagaa ggaggagtgc 300 ctgtttgtcg aagagttggc taaacagggt ctggcaccgg attacattac catcgatatt 360 gcgcatggtc acagcaactc tgttatagag atgatccaac gtattaaaac ccatttgccg 420 gagacgttcg tgattgctgg taacgttggc actccggaag cagttagaga actggaaaat 480 gctggcgctg acgcgactaa ggttggcatt ggcccaggta aagtgtgtat cacgaaaatc 540 aagaccggtt ttggcaccgg tggctggcag ctggctgcgc tgcgttggtg tgcaaaagca 600 gcgcgtaaac cgattatcgc ggacggcggt attcgcaccc acggcgacat cgtcaagagc 660 atccgtttcg gtgcgaccat ggtgatgatc ggctccttgt ttgcgggtca tgaagagtcg 720 agcggagaaa cgaagatcga gaacggcatc gcgtacaaag aatactttgg cagcgcgagc 780 gaatttcaga aaggggaaaa aaagaacatc gagggcaaga agatctggat tcagcataaa 840 ggttctttaa aagacaccct ggtcgagatg caccaagacc tccaaagctc catcagctat 900 gccggtggtc gcgatcttga ggcgatacgc aaggtggatt acgttatcgt gaagaactcg 960 atcttcaatg gtgacgcgat t 981 <210> 7 <211> 981 <212> DNA <213> Artificial Sequence <400> 7 atggtagttg aactaaagaa tatagagaaa atttatgaaa acggttttca tgcgctgaag 60 ggcgtgaacc tggagctgaa gaaaggtgat attctggggg tgatcggtta cagcggcgcg 120 ggtaaatcca ccctgattcg cctgattaac tgcctggaac gtccgagcag cggtgaggtg 180 ctggtcaacg gcgtgaacct gctgaaattg aaaccgaaag agcttcaaaa ggcgcgccag 240 aagatcggca tgatttttca acacttcaac ctgttgagtg ctaaaaacgt tttcgaaaat 300 gttgcgtttg cgttggagat agcgcgctgg gaaaagaaca aaattaagag ccgtgtgcat 360 gaactgttag agttggttgg tcttgaggac aagatgcact tctacccaaa acaactgtct 420 ggtggtcaga agcaacgtgt tgcgattgct cgtagcctgg caaattgccc ggatctcttg 480 ctgtgtgatg aagccaccag cgcgctggac agcaaaacca cgcattcgat cctgaccttg 540 ttgtctggca ttcagaagaa gctggacctg tcgatcgtat ttatcaccca tgagatcgag 600 gtggtgaaag agctgtgtaa tcagatgtgc gttatttcca gcggtgaaat cgtggaacgt 660 ggtagcgttg aggagatctt cgccaacccg aagcacgccg ttaccaagga gctcttaggc 720 atccgtaatg aacacgctga taaaaaatcc caggatgttt atcgtattgt gttccttggt 780 gagcacctgg acgaaccgat tatctcaaat ctgatcaaac gttttaaaat cgacgttagc 840 atcatctctg gtaacattga agaacttact acgaaggaca tcggctacct ggtcgtaaga 900 ttcctgggca acaccgctga aacgcagcgc gcactggagt atctgaatgc actgggcctg 960 caagtcgaga aactcaagga t 981 <210> 8 <211> 981 <212> DNA <213> Artificial Sequence <400> 8 atgggaacac taatagaaaa atggtttggg ttcagccaga ttcgtgaaga gcttgaggcc 60 cggattagcg aacttgagga tgaaaacacc gagctgttac gtgaacgcga gtacctggcg 120 gcggaaacct ccgaactgaa agacgcgaat gaccagctaa gacaaaaaaa cgacaaattg 180 tttatcacca aggacaaact cacgaaggag aataccgaac tgttcgccga aaacgaaagc 240 ctgtccgtga aaatctccgg tctagagcac agcaatgacc aactgtggca gaataataac 300 aagctgacca aagaaaaggc ggaattaaaa actgaaaagg acatcttggc taaagagaac 360 acgcgtttgc tggcggcacg tgatcgtctg accgaggaaa agcgtgaatt gaccactgag 420 aaggagcgcc tcaagcgcga gaacacggag ctgacccata aaatcaccga gttgaccaaa 480 gaaaacaaag cgttgactac ggagaacgac aagttgaacc atcaggttac cgctctgacg 540 aacgagcgtg acagcctgga acaagaacgc gcacgtctcc aggatgctca cggctttctg gagaagcgct gcaccaatct gagaaggag aaccagcgtc tgaccgataa gctgaaacag ctggagtctg cccaaaagtc cctggagaac accaataacc agctgcgcca ggcgctcgag 780. aatagcaacg tgcaactggc gcaggcaaaa gaaagatcg ccattgaaaa gtctgaactg gagcgcgaaa ttgcgcgtct gagtcgctg gagggcatgg aagcaaaatc ggatttagat cttcacaatc gtcgtttggc gagcgctaat gaggacctga aacgtcaaaa ccgtaaattg gaagagga acatcgcgct gaggacgt gttgatggtc tgaacgaaca gctgagcaaa ctgcaaccgc aaaaaccgca a <210> 9 <211> 984 <212> DNA <213> Artificial Sequence <400> 9 60. atggaacac tacacaatgc tttgttaaaa tggtatgagg agttcggtag aaaaggtttg ccgttccgca acctgaaggg catcaacgct ccgtacgagg ttatatcag cgaagtgatg agccaacaaa cgcagattaa caccgttatt gagcgcttct actcgccatt cctggaggcg ttcccgacct tgaaggactt ggcaaatgcg cagctggagg aggtgctgct gttatggcgt 240 ggtctgggct attactctcg tgcgaaaaat ctgaagaagt ccgcggaaat ctgcgtcaaa 300 gagcaccaca gccagctgcc gaatgactat cagagcctcc tgaagctgcc tggtattggt 360 gcgtataccg ctaatgcgat tctgtgtttt ggttttcgcg aaaaacgtgc gtgtgttgat 420 gcaaatatta agcgcgtgct gttgcgtctg tttggtcttg atccgaacat ccacgccaag 480 gacttacaga ttaaggcgaa cgaatttttg aacctgaacg aatcatttaa ccataaccag 540 gcactaatcg atttaggcgc gctgatttgc agccccaaac cgaaatgcgc catctgtccg 600 ttcaacccgt actgcctggg caaaaaccac ctggagaagc atactttgaa gaaaaaacaa 660 gagatcatcc aagaagaacg ttatctgggt gtcgtgatcc aaaacaacca aatagcgtta 720 gagaagattg aacagaaatt gtacttgggc atgcatcatt ttccggatct gaaagaaaat 780 ttggagtgca aactgccatt cctgggcgct atcaaacact cccataccaa atttaaactt 840 cacctgaacc tgtactctgc cgcaattaaa gacctgaaga atccggttcg tttctactcc 900 ctcaaggacc tggaaaccct gccgatcagc agcatgacgc tgaagatcct gaatttctta 960 aagcagaaga acctgttcgg cggt 984 <210> 10 <211> 984 <212> DNA <213> Artificial Sequence <400> 10 atgacatata aagaaaggct aatacacgag aagattctga aacaggatga taagggcttc 60 aagacggaac tgcgtatttt gtcgatcttc atcgtggaaa gtctggttaa cattttaggc 120 tttattttgg ccaagatgcc gcactcttgg ttcctgcgtt gtatcaaggc agttgcctgg 180 ctgatgaaga cctttgataa gtgccgttac ttcgacgcga aagctaatct ggatttcgtg 240 ttcggtgata gcaaaagcga agaggaaaaa aagcgcatca tcaagaaagg ttacgagaat 300 tttgcgttca ttatcttgga gacgatccgt gttatcttca tcccgaagga cgagtacgac 360 gcgcgtttta ccctgatcaa cgaagagaac gtgtggaaat ccttaaataa agagggccag 420 gcgatcacgc tgtgcatgca ttttggctac tgggaagcgg tgggtactac tctggcgcaa 480 tattacgaga actatggtcg tggttgtctg ggccgtctga ccaaatttgc tccgattaac 540 cacatgatta tgtctcgtcg tgaagcgttt ggtgttcgtt tcgtgaataa gattggcgca 600 atgaaagaac tcattaaaat gtataaccag ggtaatggtc tggtcggtat tttggtagat 660 caaaacgtcg tgccgaaaga cggcgtcgtg gttaaattct ttgaccgcga tgcgacccac 720 accaccatcg ctagcattct gagcagacgt tacaatattg acatccagcc agttttcatc 780 gacttcaacg acgattatag ccactacacc gcaacctatt acccgagcat ccgcagccag 840 attaccgata acgcccaaaa cgacatcctg gagtgcacgc aggcgcaggc gagcctttgc 900 gaagaggtta tccgcaacca tccggagtca tatttttggt ttcatcgtcg cttcaagtcc 960 acccacccgg aaatttacca acgg 984 <210> 11 <211> 984 <212> DNA <213> Artificial Sequence <400> 11 atggatttta aaaataaaaa gtggctattc ttggcgccac tagcgggtta taccgatttg 60 ccgtttagaa gcgttgtcaa gaagttcggc gtggacgtta ccacgtctga gatggtcagc 120 agccatagcc tggtttatgc cttcgacaag acctcgaaga tgctggaaaa aagcccgttg 180 gaggaccact tcatggcaca gattagcggt tcgaggagt ccgtggttaa agaggcagtc 240 gagaagatta atgcgttgga acacgtgaac ggcattgact ttactgcgg tgtccggct 300 ccgaaagttg ctaatcacgg caatgtagt ggcctgctga aggacttaa ccactttggtt 360 aagctgctca aaaccattcg tgagacacc agcaaaaga tcacgtccgt gaggtgcgt 420 ctgggtttg aaaaaaaaat cccgaaagag attgcgcatg cactgaatga tgcgccggtt 480 gattacgtag tggtgcatgg ccgcacccgt agcgataaat accagaagga caaatcgac 540 tacgaatcca ttgcgctgat gaaaaaaatc ctgaaaaagc cggtgattgc gaacggtgag 600 atagactccg taaagaaggc cttcgaggtg ctccagatca cccaagctga tggtctgatg 660 atcggccgtg cggctttacg tgcgccgtgg atctctctggc agatccgcaa caaccacg 720 aaactgcctg cggtcgtgaa gaaggatctg gtgctggagc acttgataa gatggttgag 780 ttctacggcg acatgggtgt tatcatgttt cgcaaaacc tgcatgcgta tgcgaaggt 840 gaatgcaag ccagcgcatt ccgcaactgc gttaacactc tgaccgaat caaagcatg 900 cgtgaatcga ttgaagagtt ctttaatcag gagatgttgc aaagcgaagt gccgctgtgg 960 gttgaactga atcaaaaatc tgtt 984 <210> 12 <211> 984 <212> DNA <213> Artificial Sequence <400> 12 atgagtggat ttaatccact aaactcaccc ttagtcgcga gctccagttt gtctctgaaa 60 gaagcgtatt atttggagaa actgtctctt aagaagggct tcaagatcca ctacaaaatg 120 accaaggaca gcttgaatct gctggagaaa agcgatctgt gcgttttgtt tggtggtttt 180 agcaacgcgt gcctgaacga aaacgaacgt tggattctcg aaagcatcag ccactcgaag 240 cgcccatacg cattactgcg tccgctgcaa gatacccgtg atctgcaaga aaattgtctg 300 ttcgcgagct atgaaatcca caccgaagct gcgatccttg ccctgattct gcgcggtatc 360 ttggagcaga cgagccagct gaagggccat gttctggaaa aaatcgatgt aggctacctc 420 tcctccgaag cgaacatgag cgaggaggaa ctgcaagagc tgatcgcttt gatcgtgaaa 480 gcaaaaaagc gcgcacttgt gctgaatcgt gaaattacca aacacgcgaa caacgcgttc 540 ctgtataccc tgttgtccga gctgcaaaac tatctggaga tcctgcatat tccgtgttat 600 gacagctctg ctaccaccgc cttttacgac tttaaggacc aggagtggct gctggagacg 660 gctttcaaag aaggcatctt accgtttaag tcgcaactgc agagcaagga cctggaactg 720 ttggagcgta tttcggaggc gaatggtagc ttcgtgtacg ttagctacaa aagcctcgag 780 actccgaaac tgtcgttcag caagcagttt aagatcgcca acaaaattga acattctaag 840 gcgggtttcc agattagcaa tcagaccctg gagtgcgaat tggaggagaa cccgcatctg 900 aaaggtctga tcgcgattct tgagggcgca ttctttgatg catacccgta catccctatt 960 ctgtcccaca gccagggtat ttcc 984 <210> 13 <211> 984 <212> DNA <213> Artificial Sequence <400> 13 atgcacacac taatagagag gttagaaaaa gtgaccaaca gcaaagaatt agaggaagcg 60 cgtctgaacg ccctgggtaa gaagggtgtg ttcgccgata aattcaacca gctgaagcac 120 ctgaacggcg aggagaagaa cgcgttcgct aaagagatcc accactacaa gcaagcgttt 180 gaaaaggcgt tcgagtggaa aaagaaggca attatcgagc tggagctcga ggaacgcttg 240 aagaaagaga agatcgatgt ttccttgttt aacgcgatta agaccagcag ctcacaccca 300 ctgaactata ccaaaaaacaa aatcatcgag tttttcacgc cgctgggcta caaactggaa 360 attggtagcc tggtcgaaga cgacttccac aacttcagcg cgctgaacct gccgccttac 420 catccggcac gtgacatgca ggataccttc tactttaaag atcataaatt gctcagaacc 480 catacctccc cggttcagat tcacacgatg caagagcaga cgccgccgat taagatgatt 540 tgcctgggtg aaactttccg tcgtgattat gatctgacgc acaccccgat gtttcatcaa 600 atcgaaggtc tggtcgtgga ccagaaaggt aatattcgtt ttacccactt gaagggtgtt 660 attgaggact tcctgcatta tttctttggc ggcgtgaaat tacgttggcg ttcgtctttc 720 tttccgttta ccgaaccgag cgcagaagtt gacatcagtt gcgttttctg caaacaagaa 780 ggctgtcgtg tgtgcagcca taccggttgg ttggaagtgc tgggctgcgg tatggttaat 840 aatgcggtat tcgaggctat cggctacgaa aatgtttctg gctttgcgtt tggtatggga 900 atcgagcgcc tggctatgct gacctgtcag atcaatgacc tgcgcagctt tttcgaaact 960 gatcttcgcg tgttggagtc cttc 984 <210> 14 <211> 984 <212> DNA <213> Artificial Sequence <400> 14 atggaaaaag tatgtgtttc agcttgggga cttccgaaaa ttttggagga gcgcttgaag 60 gagaagtatg gtgatgactg ggaaaagcac gttaaggcga aagcgatcaa cgaagaggaa 120 ctggaagagc aagtcaaggc aaaagcgaaa gagcaacaga aaacccaacg tgaaaagact 180 ctgaatggtt ttttgaagaa ggtgggcctg aagaagcgcg atatgttgca gagcaccatg 240 ctgttcgatg aagttaagga ggcagacgtt ctgttccagg cggaacgtaa aatcggcgac 300 tggatttttt ccagcgctgt gtttttcttc gctttagccc tcatcgaggc catcatcatc 360 gtgtgcctgc tgccgctgaa ggagaaggtg ccatacctgg tcaccttctc gaacgcaacc 420 cagaactttg caattgttca gcgcgcggac aaaagcatcc gtgcgaatca ggctctcgtg 480 cgtcagctgg tcgctagcta cgtgaacaac cgtgaaaata tctcctccat taaagagcaa 540 aacgagatcg cgcacgaaac cattcgcctg caaagcgcgt ttgaggtttg ggatttcttc 600 gagaaactgg tgagctacga acatagcatc tacaccaata tcaacctgac ccgtaaaatc 660 tctattatca acattgccct gatcagcaaa acgcaagcga atattgagat ttccgcgcag 720 ttgttccaca aagaaaagtt ggagagcgaa aaacgttata gaatcattat gacctttgag 780 ttcgagccga ttgagatcga taccaaaagt gtaccgctga acccgacggg ttttatcgtg 840 acgggctatg acgtgactga aattgcgatt ttaaaggacc tggatgagaa gaacaaagtg 900 aaggacgatg gtgttaagtc tcgtattatc catgttgaaa agaaagatcc gcatatgtcg 960 caatataaag acgttaaaga acag 984 <210> 15 <211> 987 <212> DNA <213> Artificial Sequence <400> 15 atgaaaaggt tcgtattatt tctattgttt atgtgcgtct gtgtgcaagc atacgctgaa 60 caggattact tcttccgcga cttcaagagc cgtgatctgc cacagaaact tcacctggac 120 aagaagctct cgcaaaccat tcaaccgtgc atgcagctga atgccagcaa gcactacacc 180 tccaccggtg ttagagagcc ggataagtgc accaaaagtt ttaaaaaatc ggctttaatg 240 tcctatgact tggcgttggg ctacctggtt agcaaaaata agcagtatgg tctgaaggcc 300 atcgaaatcc tgaatgcgtg ggctaaagaa ttacagtctg tcgataccta tcaaagcgag 360 gacaacatta acttttatat gccgtacatg aacatggcgt attggtttgt gaaaaaggcg 420 tttccgtctc cggagtatga ggacttcatt aaacgtatgc gccagtattc tcagagcgcg 480 ttgaatacca atcatggtgc atggggcatc ctgttcgacg tttccagcgc cttggcgctg 540 gacgacaacg cgctgttgca caacagcgcg aaccgctggc aagagtgggt atttaaagct 600 atcgatgaaa atggtgttat tgcgagcgcg atcacccgta gcgacacctc cgactaccac 660 ggcggtccga ccaaaggtat caagggcatc gcctacacga attttgcatt gcttgcgctg 720 acgattagcg gcgaactgct gttcgaaaac ggctacgatc tctggggtag cggtgctggt 780 aaacgtctga gcgttgcgta caacaaggtg gcaacttgga ttctgaaccc ggagacgttt 840 ccgtatttcc agccgaacct gattggcgtg cataacaacg cgtacttcat catcctggca 900 aagcactaca gctcaccgtc tgcgaacgag ctgctgaaac aaggtgatct gcatgaagat 960 ggctttcgtc tcaagctgcg ttcccct 987 <210> 16 <211> 987 <212> DNA <213> Artificial Sequence <400> 16 atgactacaa aaagggtaaa tacggctaca aacaaaatta tgaccctgaa cacctttttg 60 gacacgtgct ttctgttgtt catcagcatc ctgttttatc tgtcgatccc gatttatccg 120 aataaagttg tcgtcgtgcc gcaaggtagc ctgaagaagg ttttctttag cctgaaagag 180 cagggtgttg atatgaacgc tctggacttg ttgttcctgc gcctgatggg tatgccgaag 240 aagggctaca ttgatatggg cgacggcgcg ctgcgcaaag gtgacttctt ggttcgtctc 300 atcaaggcaa aagcggcgca gaagagcgcg accctgattc cgggtgagag ccgttatttc 360 ttcacccaga tccttagcga aacgtaccaa ctggaaactt cagatttgaa ccaggcatat 420 gaatctatcg cccctcgttt aaacggcgaa gtgatcgagg atggcgtgat ttggccggac 480 acctatcatt tgccgctcgg cgaagatgca tttaaaatta tgcaaacctt aattggtcag 540 tccatgaaaa agcacgaggc gctgtccaaa caatggctgg gctattacca taaagaggag 600 tggtttgaaa aaatcatcct ggcgtctatc gttcagaaag aagcggccaa tgtggaagag 660 atgccgctga tcgcgtccgt gattttcaac cgtttgaaga agggtatgcc actgcagatg 720 gatggtgcgc tgaactacca agagttctcc catgctaagg tgaccaaaga acgcattaaa 780 accgataata ctccgtacaa tacctacaag tttaaaggcc tgccgaagaa cccggttggt 840 tctgtcagcc tagaggcgat ccgtgccgtt attttcccga agaagaccga cttcctgtac 900 ttcgtaaaaa tgccggacaa gaagcacgca tttagcgcta cgtacaaaga gcacctgaaa 960 aacattaacc tgagcaataa ccacttt 987 <210> 17 <211> 987 <212> DNA <213> Artificial Sequence <400> 17 atgcagcaaa ggcacctagg acccttaaaa gtgggtgcgt tggcgttggg gtgcatgggt 60 atgacctacg gctacggtga ggttcacgac aagaaacaga tggttaagct gatccacaag 120 gcgctggagt tgggcattaa cttttttgat actgcggaag cgtatggtga ggacaacgaa 180 aagctgctgg gcgaagcgat taagccattt aaagacaagg tggtggtcgc gagcaaattc 240 ggcatctact acgcggaccc gaatgataaa tacgctacga tgtttctgga tagcagccct 300 aaccgcatta agtccgccat cgaaggtagc ttgaaacgtc tgaaagtcga gtgcattgac 360 ctgtattacc agcatcgtat ggacaccaat accccgatcg gtgaagttgc cgaagtgatg 420 cagctgctga tcaaagaggg taaaatcaaa gcgtggggca tgtccgaggc aggtctgtct 480 agcattcaaa aggctcacca gatttgtccg ctgtctgcgc tccaaagcga gtatagcctg 540 tggtggcgtg aaccggagaa ggagatcctc ggcttcttgg agaaagaaaa gattggcttc 600 gtggcgtttt ccccgctggg caagggcttc ctgggtgcaa agttcgaaaa aaacgccacg 660 ttcgcatcgg aagacttccg cagcgtttct ccgcgtttta accaagaaaa cctggcaaag 720 aactatgctt tggttgaact gatccaagat catgcgcacg ccaaaggtgt taccccggca 780 cagctggctt tgtcctggat tctgcatacc cagaaaatta tcgtgccgct gtttggcact 840 accaaagaga gtcgcttaat cgagaatatt ggtgctctgc aagttagctg gagccagaag 900 gagcttgaga tcttccagaa ggagttaacc gcgatcaaga ttgagggtgc gcgttatccg 960 gaacgtatca acgaaatggt aaatcaa 987 <210> 18 <211> 987 <212> DNA <213> Artificial Sequence <400> 18 atgaattata taggatcaaa atacaagcta atcccgttca tcaaagagaa catccatgcg 60 gtggttggtg acaatctgag tggcaccatt ttttgtgatc tgttcgcggg tactggtatt 120 gttggccgtg cgtttaagaa ggctgtgaat aaagtgatta gcaatgatct tgaatattac 180 tcctttgttc tgaaccaaaa ctacatcggc aacattcagg agatcccgaa tcaagaagag 240 ttgattaacg agatcaacag cgtcgcgctg aagaagggtt ttatctactc gtactattct 300 ttaggtggta gcagcagaca gtacttcagc gaaaccaatg cgcaaaagat cgacgctatg 360 cgtctcaaga tcgaagagct gaaactgtct cagaacatcg acaactgcac ctattacttt 420 ctgctggcga gcctgctgga gagcgcggac aaagtggcaa acactgcgtc cgtctatggc 480 gccttcctga aacgtctgaa aaagtctgcg cagaaggaac tgatcttgaa gggcgccgac 540 ttcgatttgt cgctgaatgc taatgaagtt taccaacagg atgctagcga gcttatcggc 600 aaaatcagcg gtgatattct gtatctggac ccgccgtata acgctcgtca atatggcgcg 660 aactatcact tgttgaatac cattgcaatt tacaccccgt ttgcaccgaa aggcaaaacg 720 ggtctgccga gttatcagaa aagctctttc tgcagccgcg cgaaaattct gaatgccttc 780 gaaaacctga ttaagaccgc gcgttttaaa tatattttct tatcctacaa caacgaaggt 840 ctgatgagcg agacggaaat cggtaatatc ctcaagaaat acggcacgta ctcccttatg 900 accaagacct acatgcgctt caaagcggac aacaagcgca cccataaagc cgcacacacc 960 aaagagtgcc tgcacattct catcaag 987 <210> 19 <211> 987 <212> DNA <213> Artificial Sequence <400> 19 atgtcagctt atataattga aacactaata aagatcctta tcttggttgc ggttttcagc 60 gccctgggtg gattcgcaac ctacatcgaa cgtaaagtgc tggcgtattt tcagcgtcgc 120 ctgggtccgt gttatgtggg tccgttcggc ctgctgcagg ttgcggcaga tggtatcaag 180 ctgtttacga aagaggacat cattccacaa ggtgcaaata agtttatctt taccctggct 240 ccaattattg ctatggtctc tgcgtttgtt agcatggcac cgattccgtt ttttccgaac 300 tttactctgt tcggttacga gatcaagccg ctgatcagcg acattaacat cggcttcctg 360 ttcttcctgg cggtgggcag cgcgggcatc tacgcaccga tcctggctgg tttggcgtcc 420 aacaaaagt acagcctgat tggttctgct cgtgcaacga ttcagttgtt gtcctttgaa 480 gtggtgtcga ccctgacgat attggcgcct ttaatggttg tcggctccct gagcctggtc 540 gagatcaatc attatcaatc cggtggcttc cttgactggc tggtttttaa acagccgctc 600 gcgttcgtgc tgttcctaat cgcgagctat gcggagctga atcgcacccc gtttgacctg 660 ctggaacatg aagccgagat tgtagcgggc tactgcaccg aatactctgg tctgaaatgg 720 ggtatgtttt tcctcgctga gtatgcccac ctgttcgcgt ttagcttcgt gattagcatt 780 gtgttcttcg gcggtttcaa cgcctggggc ttcatcccgg gcggtatcgc gattttaatc 840 aaagcaggct tctttgtttt tctgtcaatg tgggttagag ccacctaccc gcacgttcgt 900 ccggatcaat taatggatat gtgctggaaa attatgctgc cgctcgccct gttgaacatt 960 gtgttgaccg gtattatcat ccttatc 987 <210> 20 <211> 987 <212> DNA <213> Artificial Sequence <400> 20 atgcccatac tatttgattg taatgctatt gcttctcagg ttttgaagga cgaagcgagc 60 gcgcttcttg agtcagttgg ccagtttcag aaaccgaacg acctcgaggc catcgtgaag 120 ttaatcctga agtcgcaaga aaacggtggt aaactggtta ttgtgggtgt cggtaaaagc 180 gcgcttgtgg cgcagaagat cgtggcgagc atgctgtcca ccggtaatcg tagcgcgttt 240 cttcacccga ccgaggctat gcatggtgac ttgggcatgg ttgaaaagaa cgatgttgtt 300 ctgatgattt cctacggcgg ggagagcctg gagctcttga atctggtcag ccatctgaag 360 cgcctgtctc acaagatcat caccttcacc aaatctccga atagcagcct gtccaaacta 420 ggcgactatt acctgagcct gaagattcaa aaagaagcct gcccgattaa caccgcgcca 480 actacgagca cgactctgac cctggcactg ggcgacgtgc tgatggcatg tctgatgcgt 540 gcgaaaaact ttagccaaga ggatttcgcc tccttccatc cgggtggtct gttgggcaag 600 aaactgtttg ttaaagttaa agacctgtta cagaccacga acctgccgct gattgcaccg 660 tccaccagtt tcaaggacgc gctgattgaa atgagcgaga aacgtctggg cagcgcgatc 720 ctggtgaatg aggctaatga actggtcggt gttttgtctg atggtgacgt acgccgtgcc 780 ctgctgaagg gtgtcagcct gaagtccgaa gttcgccact ttgctaccct caagccgaaa 840 tcgttcaaaa accttgatgc attgttgttg gaggcgctgg agttcctgga acgtcataag 900 atccaattgc tggtgtgcgt ggatgatcac aacaaagtgt tgggcgtgct gcacctgcac 960 cagttgctgg agttaggcct gaaagca 987 <210> 21 <211> 987 <212> DNA <213> Artificial Sequence <400> 21 atgtcaaata gtatgctaga taagaacaaa gcgattctga ccggtggcgg cgcactcctg 60 ctgggcctga tcgtgttatt ttacttggcg taccgtccga aggctgaggt tttacaaggt 120 tttctggagg cgagagagta tagcgtaagc tccaaagtgc caggtcgtat tgaaaaagtg 180 ttcgtgaaga agggcgacca tattaagaaa ggcgatttgg ttttcagcat ctccagcccg 240 gaactggagg cgaagctggc tcaggcggag gccggtcaca aggctgcgaa agcgttgtcc 300 gatgaggtga agcgtggttc gcgtgatgag acgattaaca gtgcccgcga cgtttggcaa 360 gcggcaaaaa gtcaggcgac cctggcgaag gagacgtaca agcgcgtcca ggatctttac 420 gacaacggtg tggcctctt gcaaaaacgt gacgaggcgt acgcagcgta cgaaagcacc 480 aaatataatg aatcggccgc gtatcagaaa tataaaatgg cactgggcgg tgctagctca 540 gaatctaaaa tcgccgcgaa ggcaaaggag tcggctgcac tgggccaggt taatgaggtg 600 gaaagctatc tgaaagacgt caaggctacc gcaccgatcg atggtgaagt ttccaacgtt 660 ctcctgagcg gtggcgaatt gtccccgaaa ggcttcccgg tggttctgat gattgatctg 720 aaggacagct ggctgaagat cagcgttccg gaaaagtact tgaatgaatt taaagtgggc 780 aaagagtttg aaggttatat cccggcgctt aagaagtcta cgaaattccg tgtcaagtac 840 ctgtctgtga tgggtgattt cgctacttgg aaagccacca ataacagcaa cacctatgac 900 atgaaaagct acgaagtcga ggccattccg ctggaggaac tggaaaactt ccgcgtaggt 960 atgagcgttt tggttaccat caaaccg 987 <210> 22 <211> 990 <212> DNA <213> Artificial Sequence <400> 22 atgacagagg acaggctatc agctgaagat aagaagttcc tggaagtaga acgtgcactg 60 aaagaggcgg cgttgaatcc gctgcgtcat gctaccgaag agttgttcgg cgattttctg 120 aagatggaga acatcaccga gatctgctat aacggtaaca aagttgtttg ggtgttgaag 180 aacaacggcg aatggcagcc gtttgacgtg cgtgatcgta aggcgtttag cctgagccgt 240 ctgatgcact ttgctcgttg ctgcgctagc tttaaaaaga agaccattga caactacgaa 300 aacccgattt tatcttcgaa cctggcgaat ggcgagcgcg tgcagattgt cctgtcgccg 360 gttaccgtta atgatgaaac cattagcatc tctatccgca ttccaagcaa aactacgtac 420 ccgcatagct ttttcgaaga gcaaggtttt tacaatttgc tggacaacaa ggagcaagcg 480 attagcgcga tcaaggacgg cattgcaatc ggcaaaaacg ttattgtgtg cggtggcacg 540 ggttcaggta agaccacgta tatcaaaagc attatggagt tcattccgaa agaggagcgc 600 atcatctcca ttgaagatac cgaagagatc gtctttaagc accacaagaa ctacactcag 660 ctgttcttcg gtggcaacat taccagcgcg gattgtttga agagctgtct gcgcatgcgt 720 ccggatcgta tcatcctggg cgagctgaga agctccgaag cctatgactt ctataacgtg 780 ctttgctccg gccataaagg tacattgacc accctgcatg ccggttcttc cgaggaggcg 840 ttcatccgtt tagctaacat gagtagcagc aattctgccg cacgtaatat caaatttgaa 900 tcgctgatcg aaggtttcaa agacctcatc gacatgattg ttcatatcaa tcaccacaaa 960 caatgtgatg agttctacat taaacaccgc 990 <210> 23 <211> 990 <212> DNA <213> Artificial Sequence <400> 23 atgaaaagga tgctagctga atttgagaag atccaggcaa ttttaatggc gtttccgcat 60 gagttcagtg attgggcata ctgcattaaa gaagctcgcg aatccttttt gaacatcatc 120 cagaccatcg cgaagcacgc gaaagttttg gtttgtgtgc acacgaatga tattatcggc 180 tacgaaactt tgaagaacct gccgggtgtt gagattgcgc gcattgatac gaatgacacg 240 tgggctcgtg atttcggcgc aatcagcgta gagaaccacg gcgtgctaga gtgcctggac 300 ttcggcttca acgggctgggg tctgaagtat ccgagcaatc tggacaatca ggtgaacttt 360 Your aaact gcctgggttt cttgaagcat ccgctcaaaa ccatgccgta catcctggag 420 ggtggctcta ttgaatccga tggtgcgggt tctgttctca ccaacaccca gtgcctgttg 480 gagaaaaacc gtaatccgca cctgaatcaa aacggtattg aaaacatgct gaaaagag 540 ctgggtgcga aacaagttct ttggtacagc tatggctacc tgaaaggtga tgatacggac 600 agccataccg acaccctggc tcgttttctg gacaggaca cgattgtcta tagcacctgt 660 720 ttcaaaaaac ttgacggcac cccgtacaaa ctgattccac tggagatccc gaaggcgatc 780 tttgatgaga accagcagcg tctgcctgcc acctacgtta atttcttgtt gtgcaacaac 840 gccttgatcg tgccgaccta taacgacccg aaagacgcgc tgattttgga aaccctgaag 900 caacatactc cgctggaggt gatcggtgtc gattgtaata ccctgatcaa acaacacggc 960 tcgctgcact gcgtgaccat gcagctgtat 990 <210> 24 <211> 990 <212> DNA <213> Artificial Sequence <400> 24 atgttactat caaggtttca aataaaaaat ggttgtattt acggcgtttg cagctacaag 60 gcgagcaagt ccgtgtatgg ctatgaagag agcaaggctc aagtgctgaa cgccctcaac 120 accttaagcg ttcatccgat ttggcagagc aatcaagaat ctgtcaccaa aattaagggc 180 acctttgttt ttattctgga gaacgatctt cacctggacg aaaacagctt ttacaagaaa 240 ttgctcaaca gcctgattga taatgatttt ttcaaccgca gccacagcat gaccccgaac 300 caaaagcgtt ttctgtccgg ttttttcgag tctagaggta gcatcgacac ccagcgcaac 360 ttcttgactc ttgactactt cttccactcg ccgttggagt tcaaaaaatt ccattatctg 420 atcgacttct tcaacatccc gtccgaagcg ctgaacttca atttccgcga gctgcagccg 480 gaatacgcac aaggtatcaa ccagcgtaac gcgcaatttc gcatctactt ggactggtat 540 ctgcatcata ttggcctgtt taacccgtat aaggcccgta ttgcggagca cgtgtttaaa 600 accacgttgg cgcacgacgg catctactac aagttgaatt atccgcctac gaccaaatac 660 catggtaata gcttcaccga gtgcgctcac ttttatctga aaaacattta ccagcaagat 720 ctggacgaca aatcgatcga aaaactgcgt gagcagctgg gttttatcca gaagtccgag 780 gaatttcgtc gtgattccaa aatcattaat ctgtatcgtc tgtctacgcc gaatgtgtgc 840 agcgcatgct gcgatgacta tgatattaaa gaacgtagct tcctctcgct gccactgtac 900 cagatcaccc aaaacccgga tagttattac actgaaatcc acgacttctt ccgccagaat 960 cagcgtatcc gttgttttag caagtcttgt 990 <210> 25 <211> 990 <212> DNA <213> Artificial Sequence <400> 25 atggctctac ccgtatacta tgataaagac atcgacctcg gtgtgattca gagcctgcaa 60 gtgggtatta tcggctatgg tgcgcaaggt gaagcccaag cgttgaacct gagagatagc 120 aaagttaagg cgcgtattgg tctgtaccag ggctcgctgt ctgttagcaa agcgaagaaa 180 gagggcttcg aggtgttgga ggtgaaggag ctggtccaga acagcgacgt gatcatggcg 240 ttgttaccgg atgaacttca caaagaggtc ttagagaagg aggtcatccc gtttctgaag 300 gaaggccaga ttgttggctt cgcccatggt ttcagcgttc actttaacca ggttgttctg 360 ccgaagggcg ttggtgcgat cttggtggcg cctaagggtc cgggtagcgc attgcgcgaa 420 gagtatctga agaatcgtgg tttgtaccat ctgattgcta ttgagcaaga atcctccatt 480 cataatgcaa aagctgtggc cttgtcctat gctaaggcta tgggaggagg ccgtatgggc 540 gtgctggaaa ccagcttcaa agaggagtgc gaatcggact tatttggtga gcaggcggtg 600 cttgcggtg gcctggaggc tatcgttcgt atgggttttg agactctgat taagcgggt 660 tacccggaag aactcgcgta ttcgagtgt gttcatgaag tgaaactcgt tgcggatctg 720 ctgcactaca aaggtgtaga aggcttgcgc aaacacatta gcaataccgc agaatttggc 780 gcgatcaaag cgcgtgaacc gatgggcaaa ctgctgaaga agcgcatgca gaaaatcctg 840 aagaagatcc aaaacggttc ttttgcaaag gacttcttgc tggagaaaag cctgaattac 900 ccacgtctga acaccgaacg taaggccctg aaagaaacga aaatcgaaca aatcggcgag atcctgcgcg caccgttcaa ccacaaaag <210> 26 <211> 990 <212> DNA <213> Artificial Sequence <400> 26 atgaaaaagg tactacccgc tttgttaatg ggttttgtag gtctgaacgc gagcgatcgt cttctggaga tcatgcgcct ctaccagaaa caaggtctgg agatggttgg ccagaagctg gacagctacc tggcggataa gagcttttgg gcagaagagc tgcaaaacaa ggacacggat ttcggctatt accaaacaa gcagtttctg tttgtggcaa acaagagcaa accgtctctg gagttctacg aaatcgagaa caacatgctg aaaaaaatca attctagcaa ggctctggtg ggcagcaaaa agggcgacaa aaccttggag ggtgacttgg ctaccccgat tggtgtttat 360 cgtattaccc agaagctcga acgtctggat caatattacg gcgtgctggc tttcgtcacc aattatccga acctgtatga tactctaaag aagcgcaccg gtcatggtat ttgggttcac ggcatgccgc tgaatggtga ccgtaatgag ttgaacacca aaggctgcat cgcgatcgag 540 aatccgttgt tatcctccta cgacaaggtg ctgaaaggtg aaaaagcgtt cctgattacg 600 tacgaagata agttttttcc gagcaccaaa gaggaattgt ccatgattct gtcttccctg 660 ttccagtgga aagaagcatg ggcgcgtggt gacttcgagc gctacatgcg tttttacaat 720 ccaaatttta ccagatatga cggcatgaaa ttcaacgcct tcaaagaata taaaaaacgt 780 gttttcgcga aaaacgaaaa gaagaacatc gccttcagct cgatcaacgt gatcccttat 840 ccgaacagcc aaaacaagcg cctgttctac gtggtttttg atcaggatta taaagcgtat 900 cagcacaaca agttgagcta cagcagtaat tcgcaaaaag agttgtacat tgagatcgaa 960 aataaccagg tcagcattat catggaaaag 990 <210> 27 <211> 990 <212> DNA <213> Artificial Sequence <400> 27 atggcacaaa atttacccac aatagctcta ctcgctaccg gtggtacgat tgccggctct 60 ggtgcgagcg cgtccctggg tagctataag agcggtgaac tcggtattaa agagctgtta 120 aaggcgattc cgtctcttaa ccgtttggcc cgtattcaag gtgagcagat cagcaacatc 180 ggctcgcaag atatgaatga ggaagtgtgg tttaaactgg ccaagcgcgc acaggagttg 240 ctggacgaca gccgtattca aggtgtggtc atcacccatg gcaccgatac tctggaagag 300 agcgcgtatt tcctgaatct ggttctgcgc tctacgaaac cggttgtgct ggtaggtgca 360 atgcgtaatg cggcatcatt atctgcggat ggtgctctga acctgtataa cgctgtgagc 420 gttgctctga acgaaaagtc cgctaataag ggcgtgttgg ttgttatgga tgacaacatc 480 tttagcgcgc gcgaagttat taagacccac actacgcaca ccagtacttt caaggcgctg 540 aactccggcg cgattggctc cgtttactac ggcaaaacgc gttactacat gcagccgctc 600 cgtaaacata ccacggaaag cgagttcagc ctgtcgcaac tgaagacccc gctgccaaaa 660 gttgacatca tctacaccca cgcgggcatg accccggatc tgtttcaggc atccttaaac 720 agccatgcga aaggtgtcgt gatcgcaggc gtgggaaacg gtaatgttag cgcgggcttc 780 ctgaaagcca tgcaggaggc aagccagatg ggtgtcgtca tcgtgcgttc tagcagagtg 840 aacagcggcg agatcaccag cggtgagatc gacgacaagg cgtttattac cagcgataac 900 ctgaacccgc agaaagcgcg cgtgctgctg caattggcgt tgaccaagac caataacaaa 960 gaaaagatcc aagagatgtt cgaagagtat 990 <210> 28 <211> 990 <212> DNA <213> Artificial Sequence <400> 28 atgaggtata tagacgatga actagagaat caaaccattt tgatcaccgg tggtgcgggt 60 ttcgtgggta gtaatctggc gttctacttc caagagaacc atccgaaagc caaagttatt 120 atcttggaca aatttcgcag caacacgctg ttctctaaca aacgcccttc cagcctgggc 180 cactttaaga acctgattgg tttcaagggt gaagttatcg cggcggatat taataatccg 240 ctcgacttga gacgtctgga gaagctgcac tttgactact tgttccacca agcggctgtg 300 agcgatacca ccatgctgga ccaggagctg gttatgaaaa ccaactatca ggcgtttctg 360 aatttgctgg aaatcgctcg ctcgaaaaag gcgaaagtga tctatgcctc gagcgcaggc 420 gtctacggta atacgaaggc tccgaatgtt gttggctcca acgagtctcc ggaaaacgtc 480 tacggcttca gcaagctgtg catggatgag ttcgtgctga gccatagcaa tgacaacgta 540 caggttggtc tacgttattt caacgtgtac ggcccacgtg aattttacaa agagaaaact 600 gcctctatgg ttctgcaact ggctctgggt gcgatggcat ttaaggaggt taaactgttt 660 gagttcggcg aacagctgcg tgattttgtc tacatcgagg atgttatcca agcatccgtg 720 aaggctatga aggcgcagaa aagcggggtg tacaacgtgg gctatagcca ggcgcgtagc 780 tataacgaga tcgtgtccat tttaaaagaa cacctgggtg acttcaaggt tagctatatc 840 aaaaacccgt atgcattctt tcaaaagcac acccaggcgc atattgaacc ggcaattctg 900 gacttggact acaccccgct gtatgatttg gaaagcggta ttaaggatta cctgccgcat 960 atccacgcca tctttaaggg ccagtgtgca 990 <210> 29 <211> 990 <212> DNA <213> Artificial Sequence <400> 29 atgcccataa ggatagctat taatggaaca ggccgtattg gcctctgcgc gattcgtgtt 60 gcttcccagc gtaaagacat cgaaatcgtg gccatcaact ccactgcgga gctggaaacc 120 ctgcttcacc tgattcgtca tgatagcgtg cacggtcact ttgaagctca actgaatgcg 180 gatcgtaccc tgaacatcgg ccactccaag aatattctgg ttctgtcgga gagagatatt 240 aacaaactgg acttcagcgc ggctaatgcg gagattatca tcgagtgcac cggtaaattc 300 aacagcttgg aagcctcctc tgcgcatttg aagaatagcg tgaaaaaggt gatcatctct 360 gctccggcac agaacacccc tacgtttgtg tatggtgtta atcataagaa ctaccataac 420 gagagcgtca tcagcaacgc gagctgcacc acgaacgcta gtgcgccact gctgaagatc 480 ttggatgaag cgtttaaggt ggaaaacgcg ctgttgacca ccattcacag ctacaccaac 540 gatcaaaacc tgttagatac caaacacaag gacatccgcc gtgcacgtgc ggcaggctta 600 aatctgattc cgacgagcac cggtgtgtcg aaagcaattt cattggttct gccgcacctg 660 ggtccgaaag tcacgggctt ggctatccgc gtgccgactc cgaatgtttc gctagtagac 720 ctgagcctga actttaagaa gagcgttagc aaagcgagcg tacagcatgc actgaaggac 780 gcgtgtaaac acgccttcaa gggtgtcgtg agcattgacg aggaacgcct ggtttctagc 840 gatttcatct cgtccccgtt cagcgcgatt gttatgacg accagattat gaccatcggc 900 gaaaaaaacg cgaaagtttt agcctggtat gataatgaga tgggttactc cgagcgcttg 960 atcgacatgg ctcagtatat cgcccaaaat 990 <210> 30 <211> 993 <212> DNA <213> Artificial Sequence <400> 30 atggaatttt atgcttcact aaaaagtata gctatgcacg tgccgagcga gcgcgtgaaa 60 aacgcggaat ttcagcagtt tctggacacc agcgatgagt ggatcgaaaa gcgcactggt 120 attaaagagc gccgttttgc taacgatgag gagaaaagct cggatttggg ggtgattgca 180 gcgaagcaag cgattgagcg tgcgcacctc acgccaaaag acatcgatct ggttgtggtg 240 gccaccttgt ccccggattt ccttgccatg ccgagcaccg catgtgttct gtctgccaag 300 ctgggcatcg agaacaaacc ggcattcgat atctctgcgg catgcaccgg ttttatttac 360 ctgctgtccg tggctaaggc gtacgttgaa agcggtatgt atgaaaatgt tctgatcgtg 420 ggcgcggaaa agaccagcag cgttctggat ttcaaggacc gtggtacgtg cattttgttc 480 ggcgatggtg cgggtgcctg cgtcatcggc cgtacgaaac gcctcaagga gtccatcctg 540 gacgtccaga ttagcgcgaa tggtaacttc tcaaattatc tgtatacccc gcgtaccctg 600 aagccgaccc cttttaacgc gaaagaggaa gcgtccgaac cgtttctgtg tatgaaaggt 660 aatgaagttt ttaagctggc agttaaaacc ttgttgaagg acgtcgagat gattcttgag 720 aaaaacgcgc tgaagccgga agatgtgcgt ttattcatcc cgcatcaggc caactttcgt 780 attatccaag ctgtgagaga acatctggac ttcaaggacg agcaagttgt tttgactgta 840 cacaaatacg gcaacacctc tgcggcgtcg atcccgatgg ctatgggtga ggcgtacgaa 900 gagggccgtc tcaagaaagg tgacctgatg ctgctggacg cgttcggcgg cggcttgacc 960 tggggtagcg cgctggtgta cttcggtggc agc 993 <210> 31 <211> 993 <212> DNA <213> Artificial Sequence <400> 31 atgaaaaggc tattttttat tcccttcata gctccgtttt tcctgaatgg tgagccgtct 60 gcgtttgatt tacaaagcgg tgcaaccaag aaagagttga agcaactgca aatcaacagc 120 aagaacttta gcaatatttt gaccaagatc cacagccagg tagaagccaa cacccaggcg 180 caggagggcc tgcgcagcgt gtatgaaggc caggcgaaca aaatcaaaga cttgaacaac 240 gcgattctgt cgcaagagga gtctctgaga gccttgaagg cgagccaaga ggtccaggcg 300 aacacgttga agcaacaaag ccagaccctg gaagatctgc gcaacgaaat tcatgcaaac 360 cagcaagcga tccagcagct ggataaacaa aacaaggaga tgagcgaact gttgaccaaa 420 ctgtcgcaag atctggttag ccagattgca ctcattcaga aagcgttgaa ggagcaggag 480 gaaaaggctg aaaagccact gaagtccaac gcaccggcga ataagactcc gtccctgaaa 540 gccgaatctc cgaaaaacca ggagggtaaa acccaggaga aagcgaagat tgagttcgac 600 aaagacctga gtaaacaaaa agagatcttt caggaggcgt tgtccttctt caagaacaaa 660 tcctacgcag aagcgaaaga acgtctgctt tggctggagg ctaattccta ccgtctgtac 720 tacgtgcgtt atgttctggg tgaggtggcg tacggcgaaa aacgttatcg tgaagccatc 780 aagtattata aagagagcgc gcttctcaac aagaaggcta gctacatgcc ggttctgtta 840 tggcataccg catggagctt caaaaagatc aaggacgacc agaattatta caaattcctg 900 aatacgctgc aacatctgta cccgtcaagc gaacaggcca aaatggcaca aaagatcctg 960 gaaaataagg agaagcacca ccacgcgaag ccg 993 <210> 32 <211> 993 <212> DNA <213> Artificial Sequence <400> 32 atgaaattat ggtttcccta tttcctagct attgtgtttt tgcatgcgct gggtttggct 60 ttgttgttta tggctaataa cgcgagcttc tacgcagccg cgtctatggc gtacatgctt 120 ggcgcgaaac acgcattcga cgcagatcac atcgcctgca tcgataatac cattcgtaag 180 ctgacccagc aaggtaaaaa cgcgtatggt gtgggcttct attttagcat gggtcactcg 240 tccgtcgtga ttttaatgac gatcatttct gctttcgcca tagcttgggc aaaagagcac 300 accccgatgt tagaggagat tggcggtgtg gtcggaaccc tggtgtctgg tctgttcttg 360 ctgatcatcg gcctgctaaa cgccattatt ttacttgatc tgctgaagat cttcaagaaa 420 tcccatagca acgagtccct gagtcagcaa caaaacgaag aaatcgaacg tctgctaacg 480 agccgtggtc tgctgaaccg ctttttcaaa ccgctgttca actttgttag caagtcctgg 540 catatttatc cgatcggctt tctgtttggt ttaggttttg acacggcgtc tgaaatcgcg 600 ctgttggctc tgagcagctc agctatcaag gtgagcatgg ttggcatgtt gtctctcccg 660 attctgttcg cagcgggtat gagcttgttc gacactctgg acggtgcgtt catgctgaag 720 gcgtacgact gggccttcaa gaccccactg agaaagatct actacaatat tagcattacc 780 gcgctatccg tctttatcgc gctcttcatc ggcctgattg aactgttcca ggttgttagc 840 gagaaactgc acctgaagtt cgagaatcgt ctgctgcgcg cgcttcagag cctggaattt 900 accgatctgg gctattacct ggttggcttg ttcgttatcg cgttcttggg tagctttttt 960 ctgtggaaaa tcaaattttc gaaactggag tcc 993 <210> 33 <211> 996 <212> DNA <213> Artificial Sequence <400> 33 atggattcag taacactagc ttgtggaaat ggcggaaaag agacgaacgc cctgatcgaa 60 agagttttca tgccgtacct gaaggagtgg attgttgcat ttgatgaaga cgctccgaag 120 tttgaagcat ctggtgaata ctgcgtgagc accgacagct ttgtcattac tccgctgatt 180 tttaatggtg gtgatattgg caagctgtgc gtttgtggta gcgcaaacga cgttagcgta 240 caaggcggcg aaccgctgta cttgaatatg ggttttatct tggaggaggg cctggagatt 300 agcctgttaa agcagattct acaatcaatt cagaaagagc tgttcaaagc gaacctaaag 360 ctgttgtcgt tggataccaa agtggttccg aaaggttccg tggataaact cttcatcaac 420 accacctgta ttggtaagat catcaagccg ggtatttcca gctatcacct gcaacaaggt 480 caggcaatta tcctgagtga caccattgcc aaccatggtg ctagcttgtt cgccatgcgt 540 aatgaaatca aactgaagac caatctggaa agcgactgcc agctgctgta tccgctgctc 600 aaaccgttat tcctgtctga cctgaagatc gacgcgctgc gtgatgctac ccgtggtggc 660 ctggcgtccg tgctgaacga atgggctaac agcagccgcg tgaaaatcgt gatcgaagag 720 gagaaaatcc cgttgaagga ggaaacgaaa ggcatctgcg agatcttagg tctggagcca 780 tacgcgctgg cgaacgaggg ggtgttcgtt ctggcgctga accagaaaga tgcgccaaag 840 gcgcttgaga tcttgaagtc taatgaaaaa gccaaaaacg cgtgtgtcat cggcaaggtg 900 tttgaaaatc cgtatccgtc cgtcgttctg aagaacgcgt ggggtttcga gcgcattttg 960 gaagttccgg agggcgaact gctgcctcgt atctgc 996 <210> 34 <211> 996 <212> DNA <213> Artificial Sequence <400> 34 atgaaaatat ttattaatgg attcgggagg atcggccgtt gtgttctgcg tgccatcttg 60 gagcgcaacg ataccaatcc gaagttggag gtgatcggta ttaatgatcc ggcaaattgg 120 gaaatcctgg cgtatctgct agagcacgat tccgtgcacg gtctgttacc gaaggaggtt 180 cgctactcca actacaaatt gattattggc agcctggaga tcccggtttt taacagcatc 240 aaggatctta agggcgtgga tgtcataatc gagtgcagcg gtaaattcct ggaaccgaag 300 accctggaga actacctgtt gttaggtgca aaaaaggtgt tgctgtccgc tccgtttatg 360 ggcgaatacg acgaaaaaca atatccgacc ctggtgtatg gtgtaaatca tttctgctac 420 cagaatcagg cgattgtttc taacgcttct tgtaccacga acgccatcgc accaatctgc 480 gcaattctgg acaaggcctt caagatcaaa gaaggtatgc tgaccacaat tcatagctat 540 accagcgacc agaaactgat tgatctggcg catccgctgg acaagagacg tagccgtgcg 600 gcggcttcga acattatccc gacgactacg aaagcggcgc tggcgctgca caaagttcta 660 ccgaatctga aaaacaaaat gcacggccac tctgtgcgtg ttccgagcct ggacgtttcc 720 atgattgacc tttcgctttt cctggagaaa aaggcgccta aggacccgat caacgacttg 780 ttgattgaag caagcaaagg cgttttgaag ggcgtgctgg aaatcgacct gaaagaacgc 840 gtgagcagcg attttatcag caacccgcac agcgtgatta ttgcgccaga tctgaccttc 900 accctggaga atatggttaa gatcatgggt tggtacgaca acgaatgggg ttattccaac 960 cgtctggtcg atatggctca atttatgtac cattat 996 <210> 35 <211> 999 <212> DNA <213> Artificial Sequence <400> 35 atgctaaaac ccatgtatta cgaatttttc ttcatctttc cgaaagagcg cgaattgttt 60 gaatcttttc tgttagacgc gacccacctc gcgttggagg aatcgtctct tgagaacctg 120 aaggctttcg atgataaaga gaccattggc tttatcagcc agtccaattg gcactatttc 180 gccacccacg atccgctaa aaaagacttg aaggagaacc tgaaagaaaa accgcctcat 240 ctgaagaact ttgttatcct gcgtagccag areacctga acaactccct gattccggcg 300 ttggaggcgt tctgcttgaa tttgaagcaa aacttgcaga gcgagttcga cttttttac 360 ctgtctcgta acctggcgag caaagattgg ctggaagcgt ataaaaagc gattctgccg 420 gtccagtgca ccaaattcta tatccatccg agctggcacc agaagccgtc tcacgtggtg 480 accaatgatt gtattatgat tgacccggca ttagcgttcg gcagcggtca tcatgaatcc 540 acgagcatgt gcctagagct gctgagcgac atcgacctga agcgcaaaaa cgcactggac 600 gttggctgcg gttccggtat cctcagtatt gcactagaga aacagggtgt tagcgctcta 660 gtcgcctgcg ataccgatag cctggctgtg gaagaaactt tgaagaactt cagcctcaac 720 cagattccac tgctggttca agataaggtg atctacggct cgacccagaa aatcgaaggt 780 cgttttgacg tgatcgtggc caatctggtt gcggacgtca tcaaaagcct gtacagcgag 840 ttcgtgcgtc tgtgtaatca taccctgatt ttgtccggca tcttggagac gcacctgaac 900 agcgtactgc aaatttacta caatggtttt gaggttctgg agcagagaca acgtaatgaa 960 tgggttgcgc tgaagctgtt gaagaagcaa ccgattaac 999 <210> 36 <211> 999 <212> DNA <213> Artificial Sequence <400> 36 atgcccaacc accaaaatat gctagataat caaaccattc tgatcaccgg tggcaccggc 60 tcgttcggca aatgttttgt gcgcaaagtg ctggacacta cgaacgccaa gaaaataatc 120 gtgtattcta gagatgaact gaagcagagc gaaatggcaa tggagttcaa cgatccgcgt 180 atgcgttttt tcattggtga cgtgcgtgac ctggaacgtc taaactatgc gttggagggc 240 gtcgatatct gcattcatgc agcggcgctg aagcacgttc cgatcgcgga atacaatccg 300 ctggaatgta ttaaaaccaa cattatgggt gcgtccaatg tcatcaacgc ctgcctgaag 360 aacgcgattt cccaagttat tgcactctcg acggataaag cggcaaaccc gattaacctg 420 tacggcgcta ccaagttatg cagcgataag ctgtttgtaa gcgcgaacaa ctttaaaggc 480 tcctctcaaa cccaatttag cgtggttcgt tatggtaatg ttgttggtag tcgcggttct 540 gttgtcccat ttttcaaaaa attggtccag aataaagcca gcgaaatccc gattaccgac 600 atccgcatga cccgtttctg gattactctg gacgagggcg tttccttcgt gctgaagagc 660 ctgaagcgca tgcatggtgg tgagatcttt gtgccgaaaa tcccaagcat gaaaatgacg 720 gacctggcga aggcgctggc tccgaatacc ccgacgaaga tcatcggcat tcgtccgggt 780 gagaagttac acgaggttat gatcccgaaa gatgaaagcc acctggctct tgagttcgaa 840 gatttcttta tcatccagcc gaccattagc ttccagaccc cgaaggacta caccttgacc 900 aagttgcacg agaaaggtca gaaagttgca ccggacttcg agtacagcag ccataataac 960 aaccagtggt tggagccgga tgacctgttg aagctgctg 999 <210> 37 <211> 1002 <212> DNA <213> Artificial Sequence <400> 37 atgttccaca aagctctaat aacatttatt gttctgtggt ttttcctgaa tggtctgggc 60 gcgtatgatt ttaaacattg tcaggcattt ttcaaaaaag cgtcactgca aaaaggtggt 120 gtggccctga aagagctgcc gaagggcgtt tatctgtact acagcaaaac ctatccgaaa 180 cacgctaaag tgattaagag cgacccgttc gttggtttgt acttgttgca atctgcaccg 240 agcgaatacg tttacaccct gcgtgacctg gacaaggacg cgttaattcg tccgatggct 300 tccattggcg acaaagaggc cctggaaact cgtctgttgg ttggccaacg tggttacgaa 360 cgctacgcac agatcagcca gaagacgcag aaaaacggtg tcatctccaa catttgctat 420 cagatgctgg gcctgggtgt cggtggcaac ggtttcatcg agacgaaatt catcaagcgc 480 tttttgaacc agcaagagcc gtattacggc gacatcggcg tgcgtttgga ggaacaccac 540 aagcgcctgg tggtggtgca atttgatcca ttcttcccga aaaatccgtt cctcaagaac 600 gatgagatcc tggccattaa ccaccagaaa atccattctc tggcggagtt cgagtgggtt 660 gttagcaatt tgaagtatca gtccctggcg aaggtggaaa tcaagcgtaa tcataaggtg 720 aaagaggtta cccttaaggt aaacaaacgg tacggcggtt ttcttctgaa agataccttt 780 ttggaacgtt acggtattgc gctcgacgaa cgttttatta ttaccaagat cggtgcgcac 840 ctgccgaagg ggctggactt cctgaagctg ggtgatcgta ttttgtgggt caattataaa 900 agcgttgcga gcaacccgaa ggcattaaga gaagctctga gcgcgccaaa gatcgagtta 960 ctggtactgc gcaaaggctt tgagttctat atcaaggtgc gc 1002 <210> 38 <211> 1002 <212> DNA <213> Artificial Sequence <400> 38 atgttatcat ttataattaa aaggatacta tgggcaatcc cgaccttgtt tggcgtatcc 60 atcatcgtgt ttatgatggt tcacttggtg ccgggtgatc cggcgttggt gattcttgga 120 gagaaggcca accaggcagc gattgacgcg ctgcgcgaac agtttggcct gaataaacca 180 ctgatcgagc aatatttctt cttcatcaac aacgtgctgc atggtaactt cggcacctct 240 atcatgaccg gtgagccggt tatgcatgag ttctggcaaa gatttccggc taccgtggaa 300 ctggcgctca ttgcgttgtt tatggcgctg gtcttgggca tctcggtggg tgtcttggcc 360 gccattaagc gctattctgt tttcgactac agcagcatga cgtttgcatt agccggcatt 420 tccatgccgg tgttctggct gggtctgatg ctcatttata tcttcagcgt tcagctgggc 480 tggctgccgg tgttcggccg tctgtcagat gtttattatt tagatggtcc gactggtcta 540 tacctgatcg acagcctgat cgcgcgtgac tacggtgcat tcatggatac cattaagcac 600 ctgattctgc cgagcattgt cttggccacc gttagcaccg ctgtgattgc gcgtatgacg 660 cgtgcaagca tggcagaagt ttccaaagag gactacgtgc gtaccgcgaa agctaagggg 720 tgcagctctt ttcgtgttat ctttgtacac accctgcgca atgcgttaat tccggttacc 780 acgatcgctg gtctgatgct ggcgggcctg ttgggtggtt ccatgatcac cgaaactgtg 840 ttcagctggc caggtattgg caaatggatt gttaatgcgc tgaaccaacg tgattttccg 900 atcatccaga gcatgagctt gatcatcgct atgatgtaca tcggcgctaa cctgctggtt 960 gatatcctgt acgcgtttat cgacccgcgt attcgcctgt cg 1002 <210> 39 <211> 1002 <212> DNA <213> Artificial Sequence <400> 39 atgggaagta tagggtcaat gggtaaaccc atcgagggct ttttggtcgc ggcgattcaa 60 tttccggtcc cgattgtgaa cagccgtaag gacattgacc acaacatcga gagcatcatc 120 cgtaccctgc atgcgaccaa agccggttac ccgggtgttg aactgattat ctttccggaa 180 tattccacgc agggtttaaa taccgcgaag tggctgtctg aagagttcct cctggacgtg 240 cccggtaaag aaaccgagct gtatgccaag gcatgtaaag aggcgaaggt gtatggcgtg 300 ttcagcatca tggaacgtaa tccggatagc aataagaacc cgtataatac tgccattatt 360 attgacccgc aaggcgagat catcctgaaa taccgcaagc tgtttccttg gaatccgatt 420 gagccgtggt atccggggga tttgggcatg ccagtgtgcg aaggtccggg tggctccaaa 480 ctggcagttt gcatctgcca tgacggcatg attccggagc tggctcgtga agcagcgtat 540 aagggttgca acgtttacat tcgtatctct ggttacagca cccaagttaa cgatcagtgg 600 attttgacga atcgtagcaa cgcgtggcat aaccttatgt acactgtaag cgttaacctg 660 gcgggctacg acaatgtttt ctattacttc ggcgagggcc agatttgtaa cttcgatggt 720 acgaccttgg tccagggtca ccgcaatcca tgggaaatcg tgaccggtga gatctatccg 780 aaaatggcag acaacgcgcg tctgtcctgg ggtctggaaa acaacatcta caacctcggc 840 caccgcggtt acgtggccaa gccgggtggt gagcacgatg cgggtctgac ctatatcaag 900 gacttggctg ctggcaagta caaactgccg tgggaagatc acatgaaaat caaagatggc 960 tcgatctacg gctacccgac caccggcggt cgcttcggca aa 1002 <210> 40 <211> 1002 <212> DNA <213> Artificial Sequence <400> 40 atggatctaa taaacgaaaa attaaataat ctggaaaata acgccaccaa aagcccgaaa 60 gaagctgtga ttctgctgaa catgggtggt ccgaacagct tgtatgaggt tggcgttttc 120 ctgaagaaca tgtttgatga tccgttcatc ctgaccatca aaaacaattt tatgcgtaaa 180 atggttggca aaatgattgt aaattcccgc attgagaaaa gtaagaagat ctatgaaaag 240 ctcggcggta agtctccgct gaccccgatt acctttgcac tgacggaaag acttaacaag 300 ctagacccgt cccgttttta cacgtacgct atgcgttata ctccacctta cgctagcatg 360 gtgctgcagg acctggcgct gaaggaggtc gaatcgttag ttttcttcag catgtatcca 420 cagtacagca gcaccaccac gcttagctct tttaatgatg cattcaacgc cttgaaaagc 480 ctggaaacct tccgtccgaa ggtgcgtgtg atcgagcgct tctacgcgtc caagaagttg 540 aataaaatca tcttgaatac catcctgaac accctgaaca accgtaagag ccaggatttc 600 gtgctgatct tcagcgtgca tggtctgccg aaatctgtta ttgacgcggg tgatacctat 660 caacaagaat gtgaacacca tgtctctttg ttgaaagagc tgatgcaaca aaagaacacc 720 ccgtttaaag aggttttgct gagctatcag tccaaattgg gcccgatgaa atggctggaa 780 ccgtccacgg aggaactcat cgagaagcac cgtaaatcgc acattatcat ttacccgttg 840 gcgtttacca ttgacaatag cgaaactctg tacgagctgg acatgcagta tcgcttgatg 900 gcggaacgtc tcgcggtgaa agagtacctg gtgtgcccgt gcctgaacga cagcattgag 960 ttcgcgcagt ttatcatcga gcgcgttaag aacctgaagg ag 1002 <210> 41 <211> 1005 <212> DNA <213> Artificial Sequence <400> 41 atgaggctag gagttaatga agctgtagag ttgagcttag gcgaactgca aaacaccccg 60 tccatttcat atttcaactc catcgtgctg tcgctgaata aagttcagaa aggtagcttg 120 tttgttgcga aagaccacac gctgattccg aaggcgctgg agcttggtgc gtatggcatc 180 ctgtataccg gtgagtaccc ggtttctgat cgcgatgtag cttggattaa actgaaggac 240 atcgagcaca gcttaaacca tctgttcaag ttctgcctcc tgaatgaacg tgtcgttggt 300 gcgttgctat ccccgatcga gctggagatc gcgagcaaaa taatggttag taatttcgtg 360 tggtgtctga aagaatcttt ggaggacctg ttcatcatcg aaggttgtaa gatcgccttt 420 ttcgacaagt tggaatggct gcatctgttt tacaaacaag aacacttgaa ggaggatctg 480 aaggaaagct gcctgatcat tcttaatcag tcctttttct gcagcgctct ggtttatgaa 540 aaacaagaat acgagttcaa gatgccgtgc attttctcg gcagcttgaa gcgcgtgatt 600 cagctgtgtg aaaagctgca aattgagttc gacctgaact tgctgggcaa gaaagaatac 660 ccgctggatc actgcaaacc gtttttcgtg aataagaacc ttgagattgc tccgtacggc 720 accaccgcgc gtgttatcgt ggcggaaacc agcaaggagt tgttcgagat gatgctgcag 780 aaagcctttg agattctgtc ttggggtaaa atcgtcgtgt tttgccgtaa aaacagcgcg 840 gcattcttca aaaagaccaa cccttatttt tacactacgc agaacaacct gaaagagcag 900 ctgaagaacc tggcatttaa ttttgcattc attcatggta tctcgagcca tcacttggaa 960 agcctgttga acccaccgtt tttcaaaaag acgccgacct tatgg 1005 <210> 42 <211> 1005 <212> DNA <213> Artificial Sequence <400> 42 atgctagtaa caaggtttaa aaaggctttc atctcctaca gcttaggtgt gctggtggcg 60 tctctgtggt tgaacgtgtg caacgcgagc gcacaagagg tgaaagttaa ggattacttc 120 ggtgaacaga ccattaagct gccggtaagc aagattgcgt atattggtag ctacgtcgag 180 gttccggcaa tgctgaatgt gtggaatcgt gttgttggtg tttccgacta tgcctttaag 240 300 accgatcaca ccgcagcact gaacgtcgag ctcctgaaaa aactgagccc ggacctcgtg 360 gtgaccttcg tgggtaatcc gaaagcagtt gagcacgcca aaaagttcgg cattagcttt 420 ctgagcttcc aggaaactac gattgcggag gctatgcaag caatgcaggc tcaggcgacc 480 gtccttgaga tcgacgcgtc gaaaaagttc gccaaaatgc aagaaacgct ggatttcatc 540 gcggaacgtc tgaagaacgt gaagaaa aagggcgtgg agttgtttca caaagctaat 600 aaaatctctg gtcatcaggc catttccagc gacatcctgg agaaaggtgg tattgacaac 660 720 gaaaatccag aaattatctt tatctggtgg atttcgccgt tgaccccgga agatgttttg 780 aacaacccga agttcgctac catcaaggcg ataaaaaaca aacaagttta taagctgcct 840 acgatggata tcggcggtcc gcgcgctccg ctaatttctc tgttcattgc gctgaaggcg 900 catccggaag cgttcaaggg cgtcgatatc aacgcgatgg ttaaggacta ctacaaagtg 960 gtatttgact tgaacgatgc ggaagttgag ccgtttctgt ggcat 1005 <210> 43 <211> 1005 <212> DNA <213> Artificial Sequence <400> 43 atgaaagaaa tactaaatgc tttatatcac caaaaggact taaatgacga ggaagtgaaa 60 aagctgttca ctctgatcat tcatgaaaaa gtgtctccgg ttcagctcgg cgcaatcctt 120 tgcgccttga agattaaagg tgaatcattc aaggaaatca gcgttgcggc gaccaccctg 180 ctggagcacg cgccgaaacc gtttaatagc ggtttagacc tgattgataa ttgcggtact 240 ggtggtgatg gtcttaagac gattaacatc tccaccatcg ccgctttgat cgcgagctcc 300 atgggtctgt ctatggcaaa gcacggcagc cgttccgtat cgagccactc cggcagcgcg 360 gatttgctgg agaacctggg cgtgaacatt gaaatgaacc cgacccaact ggagaactgt 420 tttaagcaga cccattttgg ttttctgttc gctccgctgt accatcagag cttcaaaaag 480 tctgcgccgt tgcgcaagga gctgttcacc aagacgattt ttaactgcct gggtccgctg 540 attaatccgt tgcgccctaa aatccagctg ttaggcgtct atgataagag cctgtgcaaa 600 accatggcgc tggccctgaa ggctttgggc gtcaaacgtg caatggttgt caacggcggc 660 ggtacagatg agatcgtgct gcatgatatt acccacgcgt gcgagctgaa gaacaacggt 720 attctcgagt atgacctctc cgcgaaggac ttcgatttac caccgtacga cctgaaagaa 780 cttcaaattg aaaatgcaca ggaatccacg caagcgtgtc tggacatctt ggagaacaaa 840 ggcaaagaca gccacaccat ggtggttgtg gccaatgttg cgagcctgct gtatctgtcg 900 cacaaagcga aggacttgaa ggagggggtt tctatgaccc tggaacatct gaagaccaaa 960 gctccgtacg cacacctgca gaaaatcatc cgtctgagcc acgcg 1005 <210> 44 <211> 1008 <212> DNA <213> Artificial Sequence <400> 44 atgaaactat cagaattatt gaatgcttat tcgatcgaaa ccgagttcag caatgatttt 60 gaagttcatg ccttggcgga actgaataaa gctaccccga acgacatcag ctacattgac 120 caagcgcgtt atctgaagct gcttaaggac agcaaagctg gtgcggtgtt catccgcaaa 180 aaagagtcca gcaaagttcc gaagcgcatg caggcgttgg ttgtagataa cccacatttg 240 gcgtttgcga aagcgagcca tgcgtttaaa atcccgtttt ttaagaaccc ggaatctgtt 300 aatgaaccga aacacttcga gcgcgtgacg atcatgccga acgttgtcat cggcgagggc 360 gtcgagatcg gcgagaactc cctgatttat ccgggtgtgg tgattgcaga tggtgtgaag 420 atcggcaaga actgcattct gtacccgaga gttaccctgt accaaaacac catcttggaa 480 gataacgtga cgatccacgc tggtagcgtt atcgggggtg atggtttcgg ctatgcccac 540 accgctctcg gcgaacacgt taagatcgag cacgtgggca tcgtgcgtat tcagaagaac 600 gtggaaattg gcgcaaaatac tgcgattgac cgtgcggtgt tcggcgagac actgattaaa 660 gagggtgtta aaatcgacaa cctggtgcag atcggccaca attgtgttct gggtgagcac 720 agcattgttg tgtcgcaagt tggtctgagc ggttccacca ccactggtcg taatgtcgtg 780 ttcggtggcc aggttggcat tggtggtcat ctgcatgtcg gtgagttcac ccagattggc 840 ggtaagtctg cggtgggcaa ggacctgcct ccgaatacga acttcgccgg tgcaattccg 900 gcaatggaaa ttcacgaatg gcatcatttt ctggcgcacc tgcgtaccaa ctttcgtaaa 960 cagcaaaaaa ccagcttgtt acaaaaggcc aaaggtttct tcaagtcc 1008 <210> 45 <211> 1008 <212> DNA <213> Artificial Sequence <400> 45 atgcaagatt ttaaaacaca cctagaaccc cttaaggagg gcaaaaacct gttgggtttt 60 tctggtggtc tggattcgac ctgtctgttt ttcttgttgg ttggtgaaaa tattgtgttt 120 gatattgcgc tggtggacta caacacgcag aaacaacgtc tggagatcat ccaacatgcg 180 cagaagttgg ctaaaacgca tcacaaaaag tgctatatcc atcacgcacc gaagatcgcg 240 cacaactttg agatgcaagc tcgtaaaatc cgctatgatt tcttcgagac actgattaag 300 gagcacagct ataaacatct tatccttgcg caccacctga atgatcgctt ggagtggttt 360 ctgatgcagc tgagcaaagg cgcaggtttg aacacgttgc tgtcgttcca ggcgtatgaa 420 aaacgcgaaa gctacgccat tgttcgtccg ctgctataca ccccgaagga caccctgaag 480 actttggcga aagacctgaa attcttcgag gacgacagca atagcagcct gaagttcaag 540 cgcaactgct ttcgtaagaa ctacgctaat tccctgatgc aggattacag caagggcatc 600 attcagtctt tcaaattcct ggaccaggaa aaagaacgtc tgtacccgct gaccattgtt 660 agccagatgc acggcatcac ctttttcaag tatagccaaa acgcactgtt catggtcgat 720 aaaattctga aacagaaggg ttatgtgctg tccttcagcc aaaaggagga aatcaaacgt 780 agcttcttct ccctggagat tgcccaaaag ttcatcattg aatccgataa agagcatgtt 840 tttatcgcgc tgaaaccgcc taagaccctg tctatgccga aggactttaa ggaccgtgcc 900 cgtcgtttgg acatcccgaa gcgcctgcgt ccagtgctct acgcggaatt tctcaagcaa 960 ccgacccatg attttttaac ccgttttaaa cagtcactga tggatctg 1008 <210> 46 <211> 1008 <212> DNA <213> Artificial Sequence <400> 46 atgaggtcat ttggaaatta tatgcaagaa tggctctacg gcgaaaaggg ctactatcgt 60 aaggccctga ttggccaaaa aggcgatttt tacaccagcg tttctgtttc gaagttcttt 120 ggtggcgcgg tggccttcta catcatcaag ttattggaag aagagaaact gtttctgccg 180 ttgaagatcg tggaaatcgg ctcccatcat ggtcactttt tgagcgatat cgcttcttc 240 ctgaacgcgt tgtctgtggg cgtgatggaa cagtgcgcgt tcgtgtcgtg cgaaccgctg 300 aaggagctgc aaaaactgca acgtaccatt tttaaacagg ctacccagtt ggatctgatg 360 atttgtgatc tcaaggacct cgacttcaag ggccacgaaa acgcgttcgt tgttagcaac 420 gagttattcg acgcattcgc atgtgaaatt gtcaaagaca acaaaatgct gttcatcgcg 480 catgatcaca aaggggtgtg gggtgcaatt gatgagccga cgaaagagct gctgaagaac 540 ctggacctga agcaaggttg cgccccactg tttctcgagg cgtttattaa ggacttgcta 600 gagaagctga atgaggcttc aagctgggtt tttcttagct tcgattatgg tgacgagact 660 gaacgcaaag acatgcatct gcgtgcattc aaaaaccacc aagcgctgga cttcaaggac 720 atcctgaaca acctggcgag cctgtaccag cagagcgatt tgacctatga tgttaatttt tctctggtta gattcctctt cgaaaagcac cacgcgcagt tttccttttt caagacccag 840 gctaatgccc tgttggacat gggccttatg ggtctgctgg aaacgtttag caaaagcgtc 900. ggttacgagc gctatctgaa agaggcggca aaaatcaagc cgctgattag cccgggtggt ctgggtgagc gttttaaggc gttggagttc gtgaaaaaaa ataaaatg <210> 47 <211> 1008 <212> DNA <213> Artificial Sequence <400> 47 atgaaagaaa ggatagtaaa tctagagaca ctggacttcg aaatttccca ggaggttagc ttgagaccta gcttatggga agacttcatt ggccaagaga aaatcaagag caacctgcag atttcaatct gcgctgcgaa aaagcgtcag gagtcgctag accacatgct gttctttggt ccgccaggtc tgggaaaaac ctctatttcc catattatcg ctaaagaaat ggagaccaac attack cggcagcgcc attack aagagtggtg atctcgcggc gattttgacc aatcttcagg caaaggacat cttgttcatt gacgagatcc accgtctgtc cccggcgatc 360 gaggaagttc tgtatccggc aatggaggac tttcgcctgg atattatcat cgggagcggt 420 ccggctgcgc aaaccattaa aattgacctg ccgccgttca cccttatagg cgcgaccacc 480 cgtgcaggca tgctgagcaa tccgctgcgt gaccgtttcg gtatgagctt tcgcatgcag 540 ttttacaacc cgagcgaatt agcgctcatc atcaagaagg cggctgtgaa actgaatcaa 600 gatatcaaac aagaatctgc agatgagatt gctaagcgtt ctcgtggcac cccacgtatt 660 gccctgcgct tactgaagcg cgttcgcgat ttcgcccttg taaaaaacag ctcgctgatg 720 gacttgaaca tcactctgca cgcgctgaac gaattgggcg tcaatgagct gggctttgat 780 gaagcagatc tggcgtatct gagcttgctg gcgaacgcgc aaggtaaacc ggtgggtttg 840 aacacgatcg cggccagcat gcgggaggat gaaggcacca tcgaggacgt gatcgagccg 900 ttcctgttgg ctaatggtta cctggaacgt acagccaagg gtcgtattgc aaccccgaaa 960 acccatgaac tgttaaagat cccgacgttg aacccgcaga ctctgttt 1008 <210> 48 <211> 1008 <212> DNA <213> Artificial Sequence <400> 48 atgccaaaaa ctgaaacata tcccaggcta ttagcggaca tcggcggtac gaacgcgcgc 60 ttcggcctcg aggtggcgcc acgtcaaatc gagtgcatcg aggtcctgcg ttgtgaagat 120 ttcgagagct tgtccgacgc agttcgtttt tatctgtcca agtgcaaaga aagccttaag 180 ctgcacccga tttacggtag ctttgcggtt gctaccccga tcatgggcga ctttgttcag 240 atgaccaaca accattggac ctttagcatt gaaaccaccc gtcaatgtct gaccctgaaa 300 aagctgttgg ttattaacga cttcgtggcc caagcgtatg cgatttccgc aatgcaagag 360 aacgatctgg cgcagattgg tggcattaaa tgcgagatca atgcaccgaa agcgatcctg 420 ggtccgggca ctggtctcgg cgtttcgacc ttaattcaga atagcgacgg ttctctgaag 480 gtgctgccgg gtgaaggtgg ccacgtgtcg ttcgcgccgt tcgatgatct cgagatcctg 540 gtgtggcagt atgcgagaag caagttcaac cacgtgtctg ctgaacgttt tctgtctggt 600 agtggtctgg ttctgatcta cgaagcattg agcaagcgca aaggtttgga gaaagttgcg 660 aaactgtcca aggcagaact gacgccacag atcattagcg aatgcgccct gaatggtgac 720 tacccgattt gccgtctgac cttggacacc ttctgcagca tgctgggcac cttggcggca 780 gatgtagccc tgactttggg cgctcgtggt ggagtctatc tgtgtggtgg catcatcccg 840 cgtttcattg attacttcaa aacgagcccg tttcgcgctc gctttgaaac caaaggccgt 900 atgggtgcgt ttctggctag cattccggtt catgtggtgc ttaagaagac cccgggtttg 960 gacggcgcgg gtatcgccct ggagaattac ctgttacatg ataaaatc 1008 <210> 49 <211> 1008 <212> DNA <213> Artificial Sequence <400> 49 atgcccacag ataatgacct aaaagcagct atcttggagc tattacgtga tctcgatgtt 60 ctgctcgtgg cgcactttta ccagaaggac gagatcgtgg agttggcgca ctataccggt 120 gattcattgg agcttgccaa aattgcgagc caaagcgata agaacctgat cgttttttgc 180 ggtgttcact tcatgggtga atccgtgaaa gcgctggcgt ttgataagca ggttattatg 240 ccgaaattgt cttgttgtag catggcacgc atgattgata gccattacta tgatcgtagc 300 gtgcacctgc tgaaggaatg cggtgtcaag gagttctacc cgattaccta tatcaactct 360 aacgcggaag tcaaggcgaa agttgcgaag gacgacggcg tggtttgcac cagccgtaac 420 480. gcttccaaaa tctttaacca tgctctgaag cagaataaaa aaatcttctt cctgccggac aagtgcctgg gtgaaaatct ggctttagag aacggcctga agagcgcaat tttgggcgca 540 aattcccaag aggaaatcaa aaacgctgac gtggtgtgct ataacggctt ctgctcggtc caccagctgt tcaaactgga ggacatcgag ttctatcgtc agaaataccc agacattctg atcgccgttc acccggaatg cgaaccgagc gttgtaagca acgcggattt ttccggtagc 720 acgtctcaga taatcgagtt cgtggaaaaa ttgtccccga atcaaaaagt ggccatcggc 780 accgaaagcc acctggttaa tcgcctgaag gccaaacgcc atcatcaaaa cacgtttatt ctgtcgagca ctctggcgct ctgtccgacg atgaatgaga ccaccctgaa ggacctgttt gaggtgctca aggcttaca aaatcatcgt gcatacaaca ccattgaatt gaaggacgag gttgcgcgtc tggcgaagct ggcgttgacc aaaatgatgg aactgagt 1008 <210> 50 <211> 1008 <212> DNA <213> Artificial Sequence <400> 50 atgttatggg tactatattt tttgacatca ctattcatct gtagcctcat tgttctgtgg 60 agcaaaaaaa gcatgctgtt cgtggacaac gctaacaaaa ttcagggttt tcatcacgca 120 cgtaccccgc gcgcgggtgg cctgggcatc ttcctgtcgt tcgtgttggc gtacctgttc 180 gagccgtttg aggcaccgtt taagggtttc ttcgttttcc tgggcctgtt gctcgtattc 240 ctgtctggct ttctcgagga catcaacctg agtttatccc cgaaaatccg tctgattctg 300 caagcagtcg gtgttgtgtg catcatcagc tctatgcctt tggtggtgag cgattttagc 360 ccgcttttct cgctgccgta cttcatcgcc ttcctgtttg caatctttat gctggtgggc 420 atttcaaatg cgatcaatat tatcgacggc ttcaacggct tggcttcggg tatctgcgct 480 atcacgctgc tggtcattca ttacattgaa ccgagcagct tggcctgcct gcttgcgtac 540 atggttctgg gtttcatggt cttgaacttt ccgctgggca aaatcttctt gggtgatggt 600 ggcgcatact ttctgggcct tgtgtgcggt atcagccttt tacacctgtc cttggagcag 660 aagatttctg ttttctttgg tttgaacctg atgctttatc cagttattga agttctgttt 720 tccattctgc gtcgtaagat taagcgccaa aaggcgacca tgccggataa cttgcacctg 780 cacaccctgc tcttcaagta tctgcaacag cgtagcttta actatccgaa tccgttatgc 840 gcgtttatcc tgatcctgtg taatttgcca ttcattctca tttccgtgtt cttccgcctg 900 aacccgtacg cgctgatcgc gattagcctg gtgtttatcg cgtgttatct gatgggttat 960 gcgtacctga atcgtcaggt ttgcgctctg gaaaaaagag cctttcag 1008 <210> 51 <211> 1011 <212> DNA <213> Artificial Sequence <400> 51 atgaaaatag taattgatct aatgggagct gaccacggcg tcttaccggt aatcgagggc 60 gtgagccgtg cgctggaaaa caaatcgttc agcaccgttt tggttggtga taaagacaaa 120 gcgaccccgt ttatttccaa agagctggcg agcaaagtcg agatgatcca cacccaggat 180 tacattaaga tggaagaggc ggcaactgaa gcgattaaac gtaaagagtc tagcatttat 240 ctgggcatgg atatcctgaa gaatggtgct gatgccctta tctccgctgg tcatagcggt 300 gcgaccatgg gtctcgctac gctgcgcctg ggtcgtatta agggcgtcga gcgtccggca 360 atctgtaccc tgatgccgtc tgttggcaag cgcccatccg tgttgctgga tgcgggtgct 420 aataccgact gtaaaccgga atacctgatt gattttgcat taatgggtta tgaatacgca 480 aaatccgttc ttcactacga cagcccgaaa gtgggtctgc tgagcaacgg cgaagaagat 540 attaaaggca acatgttggt taaagagaca cataaaatgt tgaaggcgta tgacttcttc 600 tatggtaacg ttgagggcag cgacatcttc aagggtgttg ttgatgtggt ggtgtgcgat 660 ggttttatgg gcaatgttgt gttgaagacc accgaaggcg tggcctctgc gattggtagc 720 atttttaagg acgaaattaa gtctagcttc aagtcaaaga tgggcgcgct gatgctcaag 780 aacgccttcg acatcctgaa gcagaaaacg gactacgcgg aatacggcgg tgcccctctg 840 ctgggtgtga ataaatccgt gatcatcagc catggtaaat ctaacgcgcg tgccatcgag 900 tgcgcaatct atcaggcgat cagtgcagtt gagtcgcaag tttgcctgcg catcacccaa 960 gcgtttgaaa gcctgaagcc gagcgtaagc caaagcgacc agcaggacgc t 1011 <210> 52 <211> 1011 <212> DNA <213> Artificial Sequence <400> 52 atgaggttct atattatatt tacatttcta ttcattgtag gctttggtgt gtttgtgtat 60 tcaattgatc cgcaggcata cgcattcaac ctgggctcct acagctttaa cttaccgatt 120 gcggtgtggc tgatgggtgt cctgggtatg tttgctttct tcagctgggt ttttctgttt 180 aagcacaacc tgagccataa aatccgcctt tatcatgaaa aacgtgactt tgataaactg 240 cttaagcaga tcctgtcgca agatacccag aaaacctttc tgaagacgaa atttaagtct 300 gatttggcca aaaacctttc ccaaattctg gcccgttacg acttgaaggc ggacctgaat 360 actccaaata gcggctgcga aaaagttgac aatctgttca aacattatca caatattgaa 420 aacaacaccc tggagccgaa agaccatgcg aagcacagcc tggcgtacga acacgcatac ttcagcaaac gcctgaaggc gtttatccat aacgacttga agaacgcgtt cgaagttttg accaatgcgc aaattccgct ggagttacgt cgttatgcat tcatcgaaat cgcccaaaag ggtaataaaa aggaggtgtt gaaggctctg aacgctatgc aggagaacct ggataaaga 720. tgtgttaaat cgttcctgaa ggcgttcttt gagaaaagcc tgaacaccga tacgctgaag atcagcgaac tgtgcaaaaa ggtgggctat gacaagaacg actacctgaa gctcgctcag aaagcgcaga aattcttggt tccggatcag tggtttcaat ttttcgagat cctgtctcaa gaagatgaca aagctcaaaa agccttcttg ttcgtgctgc tggagctcga gatgaatgac cttgcgaaag agcacctggc ggtcttgtcc ttcgaggaat atatgctgct gaacgcgtac atggatctga caaaaaggct caaaactgg aggcattctt g <210> 53 <211> 1014 <212> DNA <213> Artificial Sequence <400> 53 atgagttcat tatttaaaat gaggatacta tcttttaaaaa aaaacaagcg cgctgtgttt 60 tccttatatc tgtttattgc attgctggcc ctgtctctgt tggctccact gtgggttaac 120 gatcgtccgt tgttcatcta taaagacaat aaagcgtatt tcccgatgtt taagaactac 180 gcggaagtcg agttcggtgg tgattttttt actccaactg actataacga cccgtacgtg 240 300 accataatca tggatctgga tagcccggct ccgacgccgc ctagcttcaa gcacctgttg 360 ggcaccgacg accaagcgcg tgatgtcctg gcgcgcttgg tttatggtta ccgtgtttcg 420 ctgattttcg gcattctgct gaccttgttt tcagtgctga ttggcgtgtc tctgggcgca 480 ttccagggtt actacggcgg tctggtggat ctggtaggtc aacgtctgag cgagatctgg 540 agcgcgattc cgatgctgtt cttgctcatc gtgatttcca gcgcgttcaa ctccaatttt 600 tggattatcc tgtttctcgt tctgctgttc tcctggatgg gtctgtcgca agttgttcgc 660 accgaatttc ttaaagcgag aaatatggac taacgaaag ccgctcgtgc gctgggcgtg 720 aacgatctga agatcatctt ttaccatgtt ctaccgaacg cactggtggc taccattacc 780 tatgtgccgt tcttaatggc agcgagtatc agcaccctcg ttagcctgga cttcttgggt 840 ttcggtatgc cgattggcag cgcgtccctt ggcgagctgg tcaaccaggg taaagacaac 900 ctgacgaccc cgcacctggc catcgttgcg ttcgttgcga ttagcttact gctgagcgtt 960 ctcgtgttta tcggcgaagg tgtgcgtgac gccttcaacg ctaatatgct gaag 1014 <210> 54 <211> 1014 <212> DNA <213> Artificial Sequence <400> 54 atgcaacact ttaatttcct atataaagat tccttgttct cgatcgcgct gttcaccttc 60 atcatcgcgt tggttatttt actggagcag gcgagagcgt acttcacgca gaaaaaaaac 120 aagaagttcc ttcaaaaatt cgctcagaat caaaacgcct acgccggtag tgaaaatctg 180 gacgagctgt tgaaacacgc caagatttcg agcctgatgt ttctggcacg cgcgtactcc 240 aaagcggatg tacagatgag catcgagatt ctcaagggtc tgttaaatcg tagcctgaag 300 gacgaggaaa aaatcgccgt tctggaccta ttggcaaaga actacttctc cgtgggctat 360 ctgcaaaaaa ccaaggacac cgttaaagag atcttgcgct tttctccgcg taatgttggc 420 gcattattga aactgctcca cgcttacgaa ttggaaaagg attatagcaa ggctctggaa 480 acgctggagt gcctggagga attagaggtc gtggaaatcg aaaccattaa aaactacctg 540 tatctgatgc atctgattga aaacaaagaa gatgtcgcga aaattctgca cgttagcaaa 600 gcgtccctgg acctgaagaa gatcgcgttg aaccatctta agagccatga tgagaacttg 660 ttctggcaag agatcgacgc taccaaacgt ctggagaacg tgatcgacct gctgtgggat 720 atgaatattc cagcgttat cctggagaag cacgcgctgt tgcaagatat tgcacgttct 780 cagggtctgt tattggacaa caaaccgtgt caagtgtttg aactggaggt gctgcgtgca 840 ctgttaaaca gcccgattaa agctcgcttg acttttgaat accgttgcaa acactgcaag 900 cagattttc cgtttgagag ccatcgttgc ccggtttgtt atcagctggc gttcatggat 960 atggtgctga agatcagcaa ggaaagctat ggttctggct tgaatgcgcg caac 1014 <210> 55 <211> 1014 <212> DNA <213> Artificial Sequence <400> 55 atgaaaagga ttttatggtt ggctctaata ctcttcttct ctccactgtt cgccaacgca 60 caaaagaccc aggagatcaa gaagacgaaa gaagcgaaaa gccagacccg tttcaacatc 120 agcaccacta aggtgattga aaaggagttc tcgcaaagcc gtagatatta cgcactgctg 180 gaaccgaacg aagccttaat cttttcccaa accttgcgct tcgacggcta tgttgaaaag 240 ctgtatgcga acaaaacgta caccccgatt aagaagggcg accgcctgtt atctgtttat 300 agcccggaac tggttagcgc gcaaagcgaa ctgctgagta gcttgaagtt caaccagcag 360 gttggtgcga tcaaagagaa gctgaagctg ctgggtctcg agaacagcag cattgaaaaa 420 atcatctcca gccacaaggt gcagaacgaa atgaccattt acagccactt taacggcatt 480 atcttcaaaa agtccccgga tttgaatgag ggttctttta tcaagaaagg tcaggagctg 540 ttccagatta tcgacctctc acaactgtgg gcattggtca aagtgaatca agaggacctg 600 gagtttctga agaacaccca taaagcgatc ctgtttgtcg aaggtattaa aggcgagcag 660 gagatcactc tggagaatat taacccgatt atcaacaaag aggacaaaat gctggaggcg 720 cgtttcaacg tgccgaatgt caaacagatt tactacccga atatgtttgc ccaagtagag 780 atctttcaga aaccgcagaa aatgaaaatt ctgcctaaag aagctgtgtt gattaagggc 840 ggcaaagcga ttgtttttaa gaaagatgat tttggtctgt ccccgttgga aatcaaggcg 900 gttcgtctgt cggatggtag ctatgaaatc ttggagggct tgaaggcggg tgaagaagtt 960 gcaaacaacg ctttgttcgt gctggatgct gatgctcaaa ataatggtga ctac 1014 <210> 56 <211> 1014 <212> DNA <213> Artificial Sequence <400> 56 atgtttttca agacatatca aaaattacta ggcgcatctt gcctgaccct gtatctggcg 60 ggttgtggta gcgattccag cgaaccgctg gttggcattg agaagaactc gttcaactcc 120 accgtgaaga taatctccaa aactgacaac attgagatcc aagatttgaa gttgaatcgt 180 ggtaattgcg agcacgacca gaattttctg gttaagctga ttcaagaaac cgcgaacacc 240 tatctgttcg ccagcgagaa agaaggct atcaaaaacc accaggcgaa aatcgcccgt 300 360 ttgctggaga acgggggttt gttcgtgtct ggtcatgact acaagtacac caaggacgacgac 420 aacccgattt atgttgttaa acgtatgctg gataacttag acagttacaa atacgagagc 480 gatgatgtgc tggacgttcc gtacgagaaa cttctggaaa tctctattgc gatcgaagat 540 accaaaaacc cgaaagatta cccgtacatt aatcttaagg aactgaaaaa actcatcgac 600 agcatcattg acgaccacgg ctatatggca gatggttttc tgaatgataa tagcaatcgc 660 gtgagcaaaa agggtctgca aattctggct aagttaaaga gcatgtggcc aagcgtcggc 720 aaattctatt ttgcgtccct gaagaggct atcccgcgtc atgcaaaaga agtcaccgat 780 aagatgatta gcagcgagga aaaatccatc aaggcgaatc aggttaagtt gaccgaagcg 840 aagcaggata ttgacaagat ggaaaaaatt atcaaggacc ttgagagcaa gaagaacagg 900 ctgtccgtgt atctcaagtt tggtgaatcg ttcacggcgc actacaagtg ccaaaacctg 960 atcgaggtgg gcgtgaaaac tgataaaggc agctggacct ttaacttcaa ccgc 1014 <210> 57 <211> 1014 <212> DNA <213> Artificial Sequence <400> 57 atgaataatt taaataacct atcagatgaa caaatcaatg gaatgatcga ctacctgcag 60 aacattctga tggagatggg cggtctgaag caagagccac cgcagaatag caactacagc 120 cacaatttgg aggagacgaa cgctcagcgt acgcagtcca cccaggagaa cagcgaaagc 180 aaggaagcgc tgctgaaggc gaaaagcggt cagattaact acgttaaaac tcgtattatt 240 cagggtttga aggagaagaa ctctccgttt tgggataaac cggagatcgt ggcgaataaa 300 gaacgcggtc acaacgctct gaacggcgag ccgtattgca atttgaacga tatgatcctt 360 gacatggaga aaaaccgttt aggctttcag agtaacgcct gggttagcct ggaagaagcg 420 aagatgctag gtgcgagcaa agaggaacgt gacaccattt tcaaagccac ccaaaacaag 480 gagatatccc cggtgcgtct gatgttcatc aaaaacaaag aaccggttcc gctggtcgat 540 aataatggtg aactcgttat cgacaagaac accaagaaac caaaacacaa acaattcgct 600 gtgattgaga acggccagaa ggtcttcaag ccggcatatc gcgacatcga accgcaggcc 660 cagtttaagt tcgtgtacaa tattgagatg tttccgagca tcaacaaaga aaaaatcaag 720 ccgctgaatt tggacaaact gtctaattat gcgtataaaa cccgcttgtt tcatcagaag 780 gattacctcc aagaacgccg tgataaaacc aacattatct acgaagacct gcatcgtgat 840 ctgtcccctg acaaccgtaa tgaggcgctg ctggagcgca tgcgtagcta taccctgttg 900 aagaacgaaa agtacaacca actggcaaac gagaccaaac aacaaacgca aaccagatcc 960 cagtcccaaa gctattacca gaaaaagaac aaggcgtcga gcggcatcga acgt 1014 <210> 58 <211> 1017 <212> DNA <213> Artificial Sequence <400> 58 atgaggcacg gagatataag cagttcaccc gacactgtag gcgtcgccgt tgtgaactac 60 aagatgccgc gtctgcatac caaaaatgaa gttctggaaa actgccgtaa tattgcgaag 120 gtgattggtg gtgttaaaca gggcttgccg ggttagatc tgatcatctt cccggaatat 180 tcgacccatg gtattagta cgaccgccag gagatgtttg acaccgctgc gtctgtgccg 240 ggcgaggaga ctgctatttt cgccgaagcg tgtaaaaaga acaaagtgtg gggtgtattc 300 agcttgaccg gtgaaaagca cgagcaagca aagaagaacc cgtataatac cttgattctg 360 gttaacgata agggtgagat cgttcagaaa taccgcaaaa ttctgccgtg gtgcccgatt 420 gaatgctggt atccgggtga caaaacgtac gtggttgatg gtccgaaagg gctgaaagtc 480 agcttgatca tctgcgatga tggtaattac ccggagatct ggcgtgattg tgcaatgcgt 540 ggcgcagaac tgattgttcg ttgtcagggt tacatgtacc cggcaaaaga acaacaaatc 600 gcgatcgtga aagccatggc atgggcgaac caatgttacg tggcggtggc gaatgcgacc 660 ggctttgatg gcgtctattc ctatttcggc cactcctcaa tttcggctt cgacggccac 720 acgctgggcg agtgcggtga ggaggaac ggtctgcagt atgctcagct tagcgttcaa 780 caaatcagag acgcgcgtaa gtacgatcag agccagaatc agctgtttaa actgctgcat 840 cgtggttaca gcggtgtgtt cgcgtctggt gacggcgaca agggcgtggc ggagtgcccg 900 tttgaatttt ataagacctg ggttaacgac ccgaagaagg cgcaagagaa cgtcgaaaaa 960 atcacccgcc ctagcgttgg tgttgctgcc tgcccagttg gtgacctccc aaccaag 1017 <210> 59 <211> 1017 <212> DNA <213> Artificial Sequence <400> 59 atgatattta ttgatgcttg tttcaggaaa gaaacgccgt ataccccgat ttggatgatg 60 cgtcaggctg gccgttactt gtctgaatat caagaatctc gcaaaaaggc gggtagcttt 120 ctggagctgt gtaaaaactc cgatctggcc accgaagtga ccttgcaacc agttgaaatc 180 ttgggcgtcg acgcagcgat tttgttctcc gacatcttgg tggttccgct ggagatgggt 240 ctgaatctgg agttcatccc gaagaagggc cctcattttc tggaaaccat caccgatctg 300 aagtccgtgg agagcttgaa agttggtgca tacaaacagc tgaattatgt ttatgatacg 360 ataagccaga cccgtcaaaa actgagccgc gaaaaggcgc tgatcggttt ctgcggttcg 420 ccgtggactt tggccaccta tatgatcgag ggtgagggtt ccaaatcgta cgcgaagtcc 480 aagaagatgc tgtacagcga accggaagtg ctgaaggccc ttctggagaa actgagcctg 540 gagctgattg agtacctgag cctgcaaatt caggctggcg tgaatgcggt tatgattttc 600 gacagctggg cgtcagcctt agagaaggaa gcatacctta aatttagctg ggattatctg 660 aaaaaaatca gcaaagaact gaagaaacgt tatgcgcata tcccggtcat cctgtttcca 720 aagggcattg gtgcgtacct ggatagcatt gatggtgagt tcgacgtttt tggcgtggac 780 tggggtacac cgctgaccgc agcgaagaag atcttgggcg gtaaatacgt gctgcaaggc 840 aacctcgagc cgacccgtct gtacgacaaa aacgctctcg aggaaggcgt agagacgatc 900 ctcaaggtga tgggaaacca gggtcacatt ttcaacctgg gccacggcat gctaccggac 960 ctgccgagag agaacgcgaa atatctggtt cagcttgttc acgctaagac ccgccgt 1017 <210> 60 <211> 1017 <212> DNA <213> Artificial Sequence <400> 60 atggaaatta cactatttga tcccatagac gcgcacctgc acgtgcgtga aaacgctctg 60 ctgaaggcgg ttctgggtta tagcagcgaa ccgttctcag ccgcggtgat catgccgaat 120 ctgagcaagc cactgatcga taccccaacg accctggagt atgaagagga aatccttaac 180 cactcttcca attttaaacc gttaatgagc ctgtacttca acgatggtct gactttggag 240 gagctgcaat gtgcaaaaga gaagggtgtt cgttttctga agctgtaccc gaagggcatg 300 accaccaatg cgcaaaacgg tacttcagat ctgttaggcg aaaaaacgct ggaagttttg 360 gagaacgccc agaaactggg ctttatcctg tgcattcatg cagaacagac cggtttctgt 420 ctggacaagg agttcctgtg ccatagcgtc ctggagacgt ttgcgctgtc cttcccgaag 480 ctgaagatca tcattgaaca tctgtctgac tggcgtagca tcgctttgat cgagaagcat 540 gataacctgt atgcgaccct gaccctacac cacattagca tgaccctgga cgacctgctg 600 ggcggtagct tgaacccgca ctgcttctgc aaaccgttga ttaaaaccaa aaaagatcag 660 gagcgcctgt tgtcgctggc attgaaagcg catccgaaaa tctcgttcgg cagcgactcc 720 gcaccgcact tcattagcaa gaaacacagc gctaatattc cggctggtat tttcagcgct 780 ccgattctgt tgcctgcgct ctgcgaattg tttgaaaagc acaacgcgtt ggagaacctt 840 caggcgttta tctccgacaa cgccaaaacc atttacggcc ttgagaacct cccgagtaag 900 aaagcgcgtc tctccaagaa accgtttatg attccgaccc ataccctgtg tttgaatgaa 960 aagatcgcga tcctccgcgg tggcgaaacg ctgtcttgga atctgcaaga aatcgcc 1017 <210> 61 <211> 1017 <212> DNA <213> Artificial Sequence <400> 61 atggatttac agcaaataga cgaactagag aacaagttcg aagaacaaga ggagcaagct 60 caagataccc cacttaaaca ggagccgagc accaaagagg tgaagatccc gaaaaagcgt 120 ggtcgtaaaa agagcttgct ggatgaagat aaaaaaaaat cgttcaacat tgcgttctcc 180 ccgtgtgtta ttaaggagct gaacgagttc ctgctcgagt tcggcagctt taaagagaca 240 agaagcacct ttattgaaga agcgctgatc cgtcatctga agcaccgcaa aaacacccag 300 gagcagaaac tgttaaagca gctggaacgt ctgcaaaaca aagaaaaggg cattatgaaa 360 accatgaatc tgaacgagtt ctttacccac aaataatct aaaagatac tccgctgaag 420 ttcaaggaca ccctggaaca agagatctca caggcgagct tggtggaaaa actgattctg 480 gcaaatattc ttgccaatat ggttttcgcc aaaatttcta atgaaaatgc accgaaaatc 540 ttgatcagcc gtctgatgtg caaattctcg ccgattgact acgaatccac gattccgtct 600 gattttaaac caatcgacga ggagaagtat gaagatgatt tggaatggct gaacgaag 660 aaggaggacc gcttgttcaa ctattacctg tttctgaatg gtatcaaaga gtccgatgtt 720 gaggaggtat ttaacgaatc tgttgaaatc tacgacgaat gcctgatcga aattgcgcag 780 aacgtgctga aggacaagtt ctcctatgac attgatctgt tgcaagtgct ggtcaagggt 840 tatgctaaag agatccgcga atttctgagc tacaagccga ttaaagagat caaggacttc 900 aaggacaagg acacggcgct gtatatcagc ttaggtaagg actacgataa ggagaaggag 960 ccgtttagca agaagctcca gcagtgcttt aaagagatct tggagagcaa aggcatc 1017 <210> 62 <211> 1020 <212> DNA <213> Artificial Sequence <400> 62 atgctacaac ccccaaaaat agtagctgaa ttgtcagcga atcacaatca ggacctcaat 60 ctagcaaagg agagcctgca cgcgatcaaa gagtccggtg cggactttgt taaactgcag 120 acgtatacgc cgagctgcat gacgttaaac agcaaagagg acccgtttat cattcaaggt 180 accttatggg ataaggagaa cttgtacgaa ctataccaaa aagcgagcac ccctctggaa 240 tggcatgcgg aattgttcga gctggcgaga aagctggacc tgggcatttt ctctagtccg 300 ttctccagcc aggctttgga gctgctggaa tccctgaact gcccgatgta caaaatcgcg 360 tcgttcgaga tcgtggactt ggacctgatc gaaaaggctg cacgcaccca aaaaccgatt 420 attctctcca gcggtatcgc tacccatacc gagctgcaag acgccatcag cctttgtcgt 480 cgtgttaata actttgatat taccctgttg aagtgcgtga gcgcatatcc gtctaagatc 540 gaagatgcga acctgcttag tatggtgaaa ctgggcgaga tctttggcgt taagttcggc 600 ctgtccgatc acaccatcgg tagcctgtgt ccgattcttg caactaccct gggtgcgagc 660 atgattgaaa aacattttat cctgaacaaa tcgctgcaga caccggatag cgcgttcagc 720 atggatttca acggttttaa atccatggtt gaggctatta agcagagcgt cctggcattg 780 ggcgaggaag aaccgcgtat caacccaaag accctggaga agcgccgttt tttcgcgcgc 840 agcttgtttg tgattaagga catccaaaaa ggtgaagctc tgactgaaaa caatattaag 900 gccctgcgtc cgaatctggg cctgcacccg aaattctata aagagattct gggtcagaaa 960 gcgtctaagt tcctgaaggc caacaccccg ctgtctgccg acgatatcga acgttccctc 1020 <210> 63 <211> 1020 <212> DNA <213> Artificial Sequence <400> 63 atgaaaatag caattgtaag gctaagtgct cttggcgaca tcatcgtgtc cgcggtattt 60 ctggcggtga ttaaagagtg cctgcctaac gcccaaattg agtggtttgt tgatgagcgt 120 ttcagcgcga ttttggagca cagcccgtac atcgacaaat tacacccaat tgcgctgaag 180 agcgctttga aaaccctcaa cccgctgaag atctttaagt tgttcaaatc cctgcgtgcg 240 tacgaatacg atatcattat cgacatgcag ggcttggtta agtctgctct gattacgcaa 300 atgctgaaag ctccgaaaaa ggtgggttt gattatgcaa gcgcgcgtga aggtctgtcc 360 atgttttttt atagccagaa agttagcatt gcatacgacg agccggtcct gaagcgcaat 420 ttcacccttc tgagccatgc gctgaactta ccgcagaagg aaattagcaa agagatttcg 480 gaatctttgt cgtctcgtgc gaaagcgttc agctatcagc cgtctccaaa gattgacgcg 540 ttgaacctga acaagaacaa gccgaagaatc ctgttcatcc tggaaccag caaaatcaac 600 aagacgtacc cgatcgaacg cttcaaagaa ctggcactga tcctggagaa cttccagatc 660 tgtctgctgt ggcatgccga cgaatacaaa gcgaccacct tatatcacgc cctgaagcac 720 cagcgtgatg tgctcttgct gccgaaattg actctgaatg aagtgaaggc tctgttattc 780 aagatggatc tgattatcgg cggtgacacc ggtattacgc atctggcatg ggcactacaa 840 aaaccgagca tcaccttgta cggcaacacc ccgatggaac gcttcaagct ggagagcccg 900 atcaatgttt ccctgaccgg taatagcaat gctaattatc acaaaaaaga tttagtatt 960 caaaacatcg aaccgaagaa gatcaaagag tgcgttctga acatcctcaa agagaaagag 1020 <210> 64 <211> 1020 <212> DNA <213> Artificial Sequence <400> 64 atgtatagga aagatttaga ccactaccta aaacagagac tgccgaaagc ggtcttcctg 60 tacggtgaat ttgatttttt catccattat tacattcaaa ccatttccgc gttgttcaag 120 tgcgataatc cggatatcga aaccagtctg ttctatgcta gcgactatga aaaatcgcaa 180 attgcaacgc tgttggagca ggatagcctg ttcggtggta gcagcttggt ggtattgaag 240 ttggacttcg cgcttcacaa aaagtttaag gagaacgaca tcaacctgtt cctgaaggcg 300 ttagagcgtc cgagccacaa ccgcttaatc attggcctgt acaatgccaa gtctgacacc 360 acgaaataca agtacacctc cgacgcgatt gttaaattct ttcagaaatc gccgctgaag 420 gatgaagcca tttgtgcacg ttttttcatt ccgaagactt gggaatctct gaagttcttg 480 caagaacgcg caaattttct ccacctggac atctccggtc atctgcttaa tgcgttgttt 540 gagatcaaca acgaagactt gggcgttagc tttaacgacc tggacaaact ggctgtgctc 600 aacgctccga ttaccctgga agatattcag gagctgagca gcaatgcggg tgatatggat 660 ctgcaaaagc tgatcctggg tctgttcttg aagaagtctg cactggacat ttatgattac 720 ctcctgaagg agggcaaaaa ggacgctgat atcctgcgtg gcttggagcg ctatttttac 780 cagctgttcc tgttcttcgc ccatattaaa accacgggtc tgatggacgc caaagaggtg 840 ctgggctacg cgccaccgaa agagatcgcg gaaaactatg cgaaaaatgc tttacgtctt 900 aaggaggcgg gttataaacg tgtttttgaa atctttcgtt tatggcacat ccaaagcatg 960 cagggccaga aagaattggg ctttctgtac ctgacctcca tccaaaaaat catcaacccg 1020 <210> 65 <211> 1020 <212> DNA <213> Artificial Sequence <400> 65 atggctaatc tagaaaactt agattggaaa aacctgggtt ttagctacat caagaccgat 60 tttcgtttca tcgccacgta taaaaacggc agctggagcc agggtggctt ggtttcggag 120 aacatgctgc agctgagtga gggcagcccg gttctgcatt atggtcaggc atgctttgaa 180 ggtctgaagg cgtaccgctc gcaaaagggc aaagccctgc tcttccgccc tctggaaaac 240 gcgaaaagat tgcaaacctc ttgtgaacgt ctgttgatgc cgaaggtctc cgaggaattg 300 ttcctgcgtg cttgcgcgga agttgtgaaa gcgaatcaga agtggctggc gccgtataag 360 agcggtgcga gcctgtatct ccgtccgttt gtgattggtg tgggggacaa cctgggcgtg 420 aaaccggcaa acgaatactt gttcatcgtc ttctgcgcgc cagttggagc gtacttcaaa 480 ggcggtattg agaaaggtgg tgcgcgtttt attacgacca tttttgatcg tgcggcgccg 540 aagggcaccg gtggtgttaa ggtgggcggc aattatgcag cgagcctgct cgctcacaaa 600 atggcaaccg aacaaggtta cgatgactgt atttacttgg acccgaccac ccacaccaag 660 atcgaagaag tgggtgccgc gaactttttt ggcatcaccc acgatgacgc tttcatcact 720 ccgcattcac cgagcatcct cccgtccatt acgaagaaat ctcttatggt tctggcgaaa 780 gagtacctga atctgaaagt cgaagagcgc gagatcttaa tggacgagct ggacgctttc 840 aaggaggctg gtgcctgcgg caccgcagcg attattacac ccatcaagga gatcgtgcac 900 aataacaaat cctatttctt cgaagcaccg ggtcatatta cgaaacgtct gtacgatctg 960 ctgctttcca tccagcaagg tgaacaggag gctccgaagg attggatttt cgaggtaggc 1020 <210> 66 <211> 1020 <212> DNA <213> Artificial Sequence <400> 66 atgatactat caattgaaag ttcttgtgat gactccagcc tggcgctgac ccgtattgag 60 gatgcgcagc tgatcgcgca tttcaaaatc tcccaagaaa agcaccacag ctcctatggt 120 ggtgttgttc cagaactggc gagccgtctg cacgcggaaa atttgccatt gctgttggag 180 cgcatcaaga tttcattgaa caaagatttt agtaagatca aagcgattgc aattaccaat 240 cagccgggcc tgagtgttac cctgatcgaa ggtctgatga tggcgaaagc tctgtcttta 300 tctttgaact taccgcttat cctggaggac cacctgcgtg gtcatgttta tagcttgttt 360 atcaacgaga agcagacgtg catgccgctg tctgttctgc tggtcagcgg cggccactca 420 ctgatcctgg aagcgagaga ttacgagaac ataaagatcg tggctaccag cctcgacgac 480 agcttcggcg aatcgttcga taaagtgtcc aagatgctgg acctgggtta cccgggcggt 540 ccgattgtcg agaagctggc tttggactac cgccatccga atgagccgtt aatgtttccg 600 attccgctca agaacagccc gaacttagcc ttcagcttct ccggtctgaa gaacgcagtt 660 cgtctggagg tggaaaaaaa cgcccctaat ctgaacgagg ctattaagca aaaaatcggc 720 taccactttc agagcgcggc gatcgaacat ctgattcagc agaccaaacg ctatttcaag 780 atcaagcgtc cgaaaatctt tggtattgtg ggtggcgcat cccaaaactt ggcgctgcgt 840 aaagccttcg aaaacctttg cgatgcgttt gactgtaaac tggtactggc tccgctggaa 900 ttttgcagcg ataatgccgc aatgattggc cgtagctcgt tggaggcata tcaaaagaaa 960 cgcttcgtgc cgttggagaa ggcgaatatt tctccgcgta ctctgctgaa gagcttcgag 1020 <210> 67 <211> 1023 <212> DNA <213> Artificial Sequence <400> 67 atggaagtat caaggaa aatatataat ccaaatagca ccgaatccgt gaatgagcgc 60 aaaatcttcg gtggtaaccc gacgagcatg ttcgatctga ataaaatcaa gtaccagtgg 120 gcagaccacc tttggaaaac catgctggcg aatacctggt ttgcagagga ggtgtccatg 180 aatgacgaca aacgtgacta cctgaagctg tccgcggaag aaaaaatcgg ctatgatcgt 240 gcattggctc aattgatctt catggatagc ctgcaggcga acaacctcat tgataatatc 300 aacccgttca tcacgtctcc ggaaattaac ttatgcctgg ttcgtcaagc gtatgaagag 360 gcgctgcaca gccatgctta cgccgtaatg gttgagagca ttagcgcgaa cacggagag 420 atctacgaca tgtggcgtaa cgacatgcag ctgaagagca aaaacgaacta catcgcccaa 480 gtttattgg aactggctaa gaacccgact gaggaagaa ttttgaaggc tttgttcgcc 540 aaccagattt tggaaggtat ttacttctac tccggtttta gctatttcta taccctggcg 600 cgctctggca aaatgttggg cagcgcgcaa atgattcgtt ttatccaacg cgatgaagtc 660 acccatctga ttctctttca gaatatgatt aacgctctgc gtaatgaaag agcggacctg 720 ttcaccccgc agttaatcaa cgaggttatt ggcatgttca aaaaagcagt cgagatcgaa 780 gcgctgtggg gtgattacat tacccagggt aagatccttg gcctgacctc ttcactgatc 840 gagcagtata tccaatttct ggcggattct cgtctgtcca aagtgggtat agccaaggtg 900 tacggcgtgc aacacccgat taagtgggtt gagtcgttca gctcgttcaa cgaacagcgc 960 tctaactttt ttgaagcgcg tgtgagcaac tatgcaaagg gtagcgttag ctttgatgac 1020 ttc 1023 <210> 68 <211> 1026 <212> DNA <213> Artificial Sequence <400> 68 atgaaaaggg tatttttatg gctaatattc gtgctggcgt ttcacaagct gttagcggag 60 aagattggtg acatcgcctc cgtcgttggt gtacgcgata atcagctgat tggttatggt 120 ctggttattg gccttaacgg cactggtgac aagtctggca gcaagttcac catgcagagt 180 atctctaaca tgctggaaag cgttaatgtg aagatctccg cggatgatat caaatctaaa 240 aacgtcgcgg cagtgatgat tacggcctcc cttccaccgt ttgcacgtca gggcgataaa 300 atcgatatcc acatctcatc tatcggcgac gccaagtcta ttcaaggtgg tactctggtg 360 atgaccccgc tgaacgccgt tgacggcaac atctacgcgc tggctcaagg tgcaatcgtg 420 agcggcaaca gcaataacct gctgagcgcg aatatcatca acggcgcgac catcgagcgc 480 gaagttagct atgatttgtt ccacaagaac gctatgaccc tcagccttaa gaacccgaat 540 ttcaaaaatg cgatccaagt tcaaaacacc ttgaacaagg tatttggtaa taaggtggca 600 attgcgttgg acccgaaaac catccaaatt acgcgtccgg aacgttttag catggtggag 660 ttcctggctt tggtgcagga gatcccgatt aactacagcg ccaagaacaa aatcattgtt 720 gacgagaagt cgggcacgat cgtttcgggt gtggacatta tggttcatcc ggttgtggtt 780 accagccagg acataaccct gaagattacg aaagatccgc tgaatgattc caaaaatacc 840 caggatctgg acaataacat gagcctcgac accgcgcata ataccttgtc gagcaacggc 900 aaaaacatta ccattgcggg tgtggtgaaa gcgctgcaaa aaattggtgt ctccgcgaaa 960 ggtatggtta gcatcctgca ggcactgaag aaaagcggcg ctatttccgc tgagatggaa 1020 atcttg 1026 <210> 69 <211> 1026 <212> DNA <213> Artificial Sequence <400> 69 atgacaaaaa agtttatgtc atggatggta gtcatcggcg cactcatctg catgctgttg 60 ggtgtgttta tttttttcac cagcatgagc gtcaaaaaat ttctgtctgc ttacttgaac 120 gcgtatctgg accagcgtcc gcatattaag ggcatgggta tcgccggtac accatttgag 180 tgcgaaggtt ttttcaagat cgcgtgtgtt tccaaagagc tgagttttct ggacagccaa 240 aacagcccga tcgtgaattt caagaacctc tcgatcaagc tgcgcagcct ggacaagagc 300 agcctgacgc tgagcgtgca ctcccaaatc aagtctccga ttctggagca ggatatgcag 360 caaaaaattt cccagatccc gctgaaggac ttgaacgcgc ttctggagaa aatgaaaccg 420 acccgtctga attgtagcct gaccttcaac gctttggacg aaaaaaccct gaatgataac 480 ctgaagtgcg atctgacgaa cgcggaaaat attctggcgt acaccttctt ccaagagggc 540 ctgatggaag cgcaagagaa cctgtccttg aagaacatct tcaagacttt gtctagcaag 600 gacgcaaaag caattgaaga actgcaagat aaattgcgct tttcagctcc gaaacttggc 660 gtttccattc aggcgcatca tctgaaaaac ctactcgaag cgttctatca ccagaacaaa 720 gagagcctgg gcttcttcag cccgtatttc agcctgcgtt ctcagacccc gagcgtttca 780 tacgagagcg cgctggcaag cctggagaat tactttatgg cattgtttca gagccacttt 840 aaggatgata ccgcgttaca acagaatttc aagggtctgc tgcaagcctt tgtttccatg 900 gctaaggaca aacgttctca gattgcgctg aacgctcagg cgaaggacaa tgccaaactg 960 acctttaacg ccctattgga gtcgttgtcc gtgaacttct tccaaagtta taaaatcagt 1020 cacgaa 1026 <210> 70 <211> 1026 <212> DNA <213> Artificial Sequence <400> 70 atgaaaatag ctgtactatt atcaggaggg gtcgatagca gctactcagc ctactcgctg 60 aaagagcagg gccacgagct ggtcggtatc tatctgaaac tgcatgcgtc cgaaaagaaa 120 cacgatctct acattaagaa cgcgcagaaa gcgtgtgagt tcctgggtat tccgctggag 180 gttctggact ttcagaaga cttcaatct gcggtttacg acgagttcat caacgcgtat 240 gaagaaggtc aaaccccgaa tccgtgtgct ctttgcacc cgcttatgaa attcggcctg 300 gctttagacc acgccttgaa gttggggtgc gaaagatcg caacggtca ttatgcgcgt 360 gttaaagaga tcgacaaat cagctacatc cagaagcac tcgataaaac caagaccac 420 agctacttcc tgtacgcgtt ggagcacgaa gtcatcgcca agctggtttt tccgctgggt 480 gatttgctga aaaaggacat caaccgctg gcgctcaatg ctatgccgtt tctgggcacg 540 ctggaaacct ataaagaag ccaggagatc tgcttgtgg agaagtctta cattgacacc 600 ctgaagaagc acgtggaggt agagaggag ggcgtggtta aaacctgca aggtgaagtg 660 atcggtacgc ataaggtta catgcagtat accattggca aacgtaaagg cttcagcatt 720 aaaggcgcat tggaaccaca tttgttgtg ggcattgatg caaaaaaa tgaactggtc 780 gtgggtaaaa aggagatct ggcgacccac tccttgaagg ctaagaacaa atccctgatg 840 aaggacttca aggatggtga atatttcatt aaggctcgct atcgtagcgt tccggcgaag 900 gcccatgtga gcctcaagga cgaggttatc gaggtgggtt ttaaggagcc gttttatggt 960 gtcgcaaagg gtcaagcgtt agtggtgtac aaagatgata ttctgttggg cggtggcgtt 1020 attgtt 1026 <210> 71 <211> 1029 <212> DNA <213> Artificial Sequence <400> 71 atggctacaa aactaactcc caagcaaaaa gcgcaactgg acgagctgtc catgtctgaa 60 aagattgcca ttttgctgat ccaagttggt gaggatacca ccggtgagat tctgcgccac 120 ctggatattg atagcatcac cgaaatcagc aaacaaattg ttcagcttaa cggtactgac 180 aagcagatag gcgcggcggt cctcgaggag tttttcgcga ttttccagag taatcagtat 240 attaacaccg gtggcctgga atatgcacgc gagttgctga cccgtacgct gggcagcgaa 300 gaggcaaaga aggtgatgga taagctcacc aaaagcctgc aaacccagaa aaacttcgcc 360 tacctgggca agatcaagcc gcagcagttg gcggacttca tcattaatga acatccgcag 420 acgattgcgc tgatcttggc gcacatggaa gcgccaaatg cagcggaaac cctgtcgtac 480 ttcccggatg aaatgaaagc ggagatctct atccgcatgg ctaatttagg cgaaatctct 540 ccgcaagtgg tgaagcgtgt gagcaccgtt ctggaaaaca agttggagtc gctgacatcc 600 tacaaaatcg aggttggtgg cttgagagcg gtcgccgaga ttttcaaccg tctaggccag 660 aaaagcgcaa aaaccactct ggcgcgtatt gagagcgttg ataacaaact ggctggtgca 720 atcaaagaaa tgatgtttac ctttgaagac atcgtgaagc tggacaactt tgcgatccgt 780 gaaatcctga aagttgctga caaaaaggac ttgagcttag cgctgaaaac gagcaccaaa 840 gacctgacgg acaagttcct gaataacatg tccagccgtg ctgccgagca gtttgtggaa 900 gaaatgcagt atctgggtgc tgtgaagatc aaggatgtag atgttgcaca acgtaaaatc 960 atcgaaattg ttcaatcctt gcaagaaaag ggcgttattc agaccggtga ggaggaggac 1020 gtgattgag 1029 <210> 72 <211> 1029 <212> DNA <213> Artificial Sequence <400> 72 atgcaagaaa ttctaatacc cttaaaagag aagaactaca aagtctttct gggtgagctg 60 ccggaatta aactcaagca gaaggccttg atcataagcg acagcattgt tgccggcctg cacctaccgt acctgttaga gcgtcttaag gcgttggaag ttcgtgtgtg tgttattgag 180 agcggtgaa agtatagaa ttttcactct ctggaaaga tcttgaacaa cgcgttcgag atgcagctga accgccattc gctgatgatt gcgctgggtg gcggagtgat ttctgatatg 300 gttggctttg cgagcagcat ttacttccgt ggtattgatt ttatcaacat tccgaccacc 360 ctgttggccc aagtcgatgc atccgtgggc ggtaaaaccg gtatcaacac cccgtacggc 420 aaaaacctga tcggtagctt tcaccaaccg aaagctgtgt atatggatct tgcgttcctg 480 aagaccctgg agaaacgtga atttcaggcg ggcgttgctg aaatcatcaa gatggctgtt tgcttcgata aaaacctggt tgaacgcttg gagacgaaag acctgaagga ctgcctggag gaagtgatct tccagagcgt father gcgcaagtgg tggtgcagga tgaaaaagag 660 cagacattc gtgcaggttt cacaccttg cacactgcaat cgaaaaggag actgactacg agcgtttcct ccacggcgag gctattgcga tcggcatgcg tatggcgaat gatctggccc ttagtctggg catgctgacc ctgaaggagt atgaacgcat cgaaaatctg 840 ctcaagaaat tcgacttgat cttccactat aaaatcctgg acctgcaaaa attctatgag 900 cgcttgtttt tagacaaaaa atcagagaat aaaaccatta agttcatcct gccaaaaggt 960 gtcggtgcat tcgaggtcgc gtcccatatt ccgaaagaaa cgatcatcaa ggtgctggaa 1020 aagtggcat 1029 <210> 73 <211> 1029 <212> DNA <213> Artificial Sequence <400> 73 atgcagaatt tactaataca agctgaaaac gcgatcgcgc tgttattcct gttgaatgat 60 aagaacctca agggcaagat cgatttaatt tacattgacc cgccttttgc cacgaacaac 120 cactttacca tcaccaatgg tagggctacg accatcagca acagcaaaaa cggtgatatc 180 gcgtactccg ataaggtcgt gggcatggat ttcatggagt tccttaagca gcgcctggtg 240 ctgctcaagg agctgctgag cgaacaaggt tcgatctacg ttcacaccga ttacaaaatt 300 ggtcattatg tcaaagttat gctggatgaa atctttggta ttcaaaattt tcgtaatgag 360 atcacccgta ttaagtgcaa tccgaagaac ttcaaacgta ttggctatgg tatattaaa 420 gacatgattc tgttctactc aaaggggaaa aacccgatct tcaacgagcc gaagatcccg 480 tatactccgc aggacttaga gaagcgcttt ccgaaaatcg acaaggacaa gcgccgttat 540 accacggtgc cgattcacgc tccgggtgag gttgaaagcg gcgagtgcag caaggcgttc 600 aaaggcatgc tgccgccaaa aggccgtcat tggcgtaccg acatcgcgac cctggagaga 660 tgggataaag aaggtctgat cgaatactcc aacaataaca acccacgtaa aaaaatctat 720 gccctggagc aggtaggtaa acgcgtgcaa gacatttggg aatttaagga cccgcaatat 780 ccgagctacc cgaccgaaaa aaacgcgcag ctgttggact tgataatttaa gacctctagc 840 aacaaggaca gcattgttct ggactgtttc tgcggttccg gcaccacgct gaagagtgca 900 ttcctgttgc aacgtaagtt catcggcatt gacaactctg atttggcgat ccaggcatgt 960 aaaaataaac tggagacgat taccaaagat ctgttcgtga gccagaattt ttacgacttt 1020 ctggtttt 1029 <210> 74 <211> 1032 <212> DNA <213> Artificial Sequence <400> 74 atggctctat tatttacagg agcatgtggg tacattggct cacataccgc gcgtgcattt 60 ttggagaaga ccaaagagaa cattatcatc gtggatgacc tgagcacggg ttttttggag 120 cacctgaagg cgctggagca ctactacccg aatagagttg tatttatcca agcgaatctg 180 aacgagacac acaaactgga cgctttcttg aataagcaac aactgaaaga tccgattgaa 240 gcaattctgc attttggcgc gaaaatcagc gttgaagaat ctacccacct tccgctggag 300 tattacacca acaacacctt gaataccctg gagctggtta agctgtgcct gaagcacgcg 360 attaagcgct tcatcttcag ctccactgcg gttgtctatg gtgaatcgtc ttcctccctg 420 aacgaagaat cgccgctgaa cccaatcaac ccatacggcg ctagcaaaat gatgtccgaa 480 cgtattctcc tcgataccag caaaatcgcg gattttaagt gcgttattct gcgttatttc 540 aacgttgccg gtgcctgcat gcataatgac tacaccaccc cgtacacgct gggtcagcgt 600 acgctgaacg ctacccactt aatcaagatc gcgtgtgaat gtgcggtggg caaacgcaaa 660 aagatgggta ttttcggcac caattatccg acccgtgatg gtacgtgcat tcgtgattac 720 atccatgttg acgacctggc gaacgcccat ctggctagct atcagacctt gttggagaag 780 aacaaaagcg aaatttataa cgtgggttac aaccagggcc atagcgtgaa ggaggtgatc 840 gagaaggtga aagaaatcag caataacgac ttcttggtcg agatcctgga caaacgccag 900 ggtgatccgg catcgctgat tgccaataac gcgaaaattc tgcaaaatac ttcttttaaa 960 ccgctgtata acaacctgga caccattatc aagagcgcac tggactggga agagcacttg 1020 ctgcgcttcc ag 1032 <210> 75 <211> 1032 <212> DNA <213> Artificial Sequence <400> 75 atgatattaa aaagggtaac agaagctcta gaggcgtaca aaaacggtga aatgctgatc 60 gtgatggacg atgaagaccg cgaaaatgag ggcgatttgg tgctggctgg catcttcagc 120 accccggaga aaatcaactt tatggctacc cacgccagag gcctgatttg cgtttccctg 180 accaaggacc tggcgaaaaa atttgaactg ccgcctatgg tttccgtgaa tgattccaac 240 cacgaaaccg cattcaccgt ctcaatcgat gctaaggagg cgcgtaccgg gattagtgca 300 ttcgagcgcc atctaacgat tgaactgctt tgcaaagaca ccaccaaacc gagcgacttt 360 gttcgtccgg gtcacatttt cccgttgatc gccaaagatg gcggtgttct ggcgcgtact 420 ggtcacaccg aggcgagcgt ggatctttgc aagctggctg gcctgaagcc ggtttcggtg 480 atctgcgaaa tcatgaaaga agacggcagc atggcgcgcc gtggcgaacaa attcttgagc 540 gacttcgctc tcaagcaaa cctgaagacc ctgtatgtct ctgacctcat ttcctatcgt 600 ctggaaaacg agagcttgct gaagatgttt tgtcaggagg aacgtgaatt tttgaagcat 660 caaacgcagt gctatacctt tttagatcat caacaaaaaa accactacgc cttcaagttc 720 aagggtgcaa aaacccacga tttggcgcca ctggtacgct ttcatccgat taagaggat 780 ttcgatttcc tgactacgga tgcgtttgag gtgtttttta aggcgttaga gtacctgaaa 840 cacgagggcg gttacctgat cttcatgaat acccatagca aagagaacaa tgttgttaaa 900 gacttcggca tcggtgcgtt ggtgctcaag aacctgggta ttaaggactt ccgtctgctg 960 tctagctgtg aggaccgtca gtacaaggcg ctgtctggtt ttggtcttaa gctggtcgaa 1020 acaattagcc tg 1032 <210> 76 <211> 1032 <212> DNA <213> Artificial Sequence <400> 76 atgaggttat atgaatcact attggagatg tgcctgaaca aggcctggga gcaccagacc 60 ctcgcgctgg agaacccgtc cgtggcctgc atggtgctgg acaagaatca tgaaattctg 120 tctctggaaa cccacaaaaa ggcaaagacg ccgcatgcgg aagttctggc ggcacaaagc 180 gctcttaaaa tcctgcgtcc gtctctgaag aacgatttgg agaaactgga ggacccgaaa 240 actttgtcgg attttctcaa aacccaccac gataacgcat tcaccgattg tgttttcctg 300 attaccttag agccgtgtaa ttcctacggc aagaccccgg catgcagcga attattggag 360 atcctgaaac cgaagcgcgt tgttatcgcc accgaagaaa acgaggcgaa aaaaggcggt 420 ctggcgcgtc tgcaaaaagc tcgcatcgag acgattattt gtcataacct ggagaataag 480 gctaaagacc tgctgttgcc gttccgcgtg atggaacaga agggccgttt caacctgttc 540 aaactggcgt tgcgcatgaa tggtgattat catcacggca aaatcaccgg tcagaaaagc 600 gtcatcttta cccacaacca acgtgcgatc tgcgataccc tgatcgtcag cggtaagacg 660 attcgcaccg acaatccgct gcttgatgcg cgtttctgcg acagttttta ccagaataag 720 aacccgaata ttgctatctt gtccaaaaga agcatcgacc cgaacagcaa ggtgttcagc 780 gcgcctaacc gtctggttaa tacctttcat gacccaaagg acctgccgtt ggagaaggga 840 tttaacttta tcgaaggtgg ttgggagctg tttgaatcac tgcgtgataa aatcgacgcg 900 ttgctgttgc acagccatgc gagcatgatt ggtgaggcgt tcaaggccct ggcgttgaag 960 actccattca aaggccgtct gctccacgct cagattttgg agaacgaagc actcctgtgg 1020 attgaaaact ct 1032 <210> 77 <211> 1032 <212> DNA <213> Artificial Sequence <400> 77 atgaaggtaa ttaaaacagc tcccctaata ccaagcgaga tcaaggtgtt ggagaaagaa 60 ggcaaccgcg tgaagatcag cctcgctccg tttgagttcg gctacgcggt tacgttggcg 120 catccgatcc gccgtttgct gctgctctcc tccgttggtt acgcgccagt tggcctgaaa 180 atcgagggtg ttcaccacga gttcgacagc ttacgtggtg tgaccgagga cgttagcctg 240 tttattatga atctgaagaa cattcgtttt atcgctaagg cactggtcgg tcaggattct 300 tctttggaga accagagcgt tgtggttgat tactcgttca aaggtccgat ggaactgcgt 360 gcgcgtgact tgaactccga acaaatcgag atcgttaatc cggagatgcc gctggcaacc 420 attaacgaag acgcgcaact gaatttcagt ctgattattt ataaaggtat gggttacgtt 480 ccgagcgaaa acacccgtga gctgatgccg gaaggttaca tgcctctgga cggcagcttc 540 accccgatta agaaggtcgt gtatgagatc gagaacgtgc tggttgaagg cgacccgaat 600 tatgaaaaga tcatctttga tattgagacg gatggccaga ttgatccgta taaagcattc 660 ctgtctgctg tgaaggtgat gagcaaacag ctgggtgtat ttggtgaacg tccgatcgcg 720 aatactgagt atagcggtga ctacgcccaa agagacgacg ccaaagatct atcagccaag 780 atcgaatcta tgaacctgtc cgcgcgctgc tttaattgtc tggataaaat tggtattaaa 840 tacgtcggtg aacttgtgct gatgagcgag gaagagctga agggcgtcaa aaacatgggc 900 aaaaaaagct atgatgaaat cgcggaaaaa ttgaacgacc tgggctaccc ggtgggcacc 960 gaattgtccc cggagcagcg cgagtcgctt aagaaacgtc tggaaaagtt ggaggacaag 1020 ggcggtaacg at 1032 <210> 78 <211> 1035 <212> DNA <213> Artificial Sequence <400> 78 atgataaaac actatctatt tatggctgta agccaggttt tcttctcgtt ctttctggtt 60 ctgttcttca tcagcagcat tgtgttgctg atctcaatcg ccagcgtgac cctggttatc 120 aaagtgagct tcttggacct ggttcagctg ttcctctaca gcctgccagg tacgattttt 180 tttatcctgc cgattacctt ttttgctgcg tgcgcgctgg gcctgagccg tctgtcctac 240 gaccacgagc tgctggtgtt cttctccctt ggtgtctctc cgaaaaagat gaccaaagtt 300 ttcgctccgc tcagcctgct agtgtccgca atcttattag tgtttagcct gatattgatc 360 ccgaccagca aatcgacgta ttatggtttt ctccgccaga agaaggacaa aatcgatatc 420 aatattcgtg cgggtgaatt tggccagaag ttgggcgact ggctggtcta cgttgataag 480 accaaaaata acagttacga taacctggtg ttgttctcta acaaaagcct gtcgcaagag 540 agcttcattc tggcacagaa gggcaatatt aacaaccaaa acggcgtttt cgagttgaat 600 ttatacaatg gccatgcgta ttttacccaa ggtgataaaa tgcgtaaggt ggacttcgag 660 gaactgcacc tgcgcaataa actgaagtcc ttcaacagca acgatgcggc gtacctgcag 720 ggcacggatt atctgggtta ttggaaaaaa gcatttggta aaaacgcgaa caagaaccaa 780 aagagacgtt tctcccaagc gatcttggtc agcttgttcc cgctggcgtc ggtattcttg 840 atcccgttat ttggtattgc taacccgcgt tttaaaacca attggagcta cttctacgta 900 ctgggcgctg tgggtgttta tttcctgatg gtgcatgtta ttagcactga cctgttcctg 960 atgacctttt tcttcccgtt tatctgggca tttgcctcct acctgttgtt tcgtaagttt 1020 attcttaagc gctat 1035 <210> 79 <211> 1035 <212> DNA <213> Artificial Sequence <400> 79 atgaaagaat ttgatctaga gtcatatgac tactatctgc cgaaagagct gatcgcctca 60 tacccggttc tcccgaaaga gaaggctaag ttgttggttt atgaacgtcg ttctcaaaag 120 atcacccata ctacgtttga gcacgtgctg gacttcttcc cgaaaaacgc attgatcgtg 180 ttgaatgata ccaaggtgat taaggcgaga ctgtttggta gcaaacacgc gttcctgcca 240 agcaaaacga ccgaggtgtt tttccatcgc ttttttaaga acaacaccgc tctgacccag 300 attaaaggta aaatcaaggt tggtgataaa attttcttcg atgcgaacta ccatgctgaa 360 gtcttggaat tgttgcacaa cggtcagcgt ctgatcgcgt tttatgacaa caaaaccccg 420 ttgaaccaag agaacattct gaaattgctt gagcagtatg gccatatgcc gctgcctccg 480 tatatcaaac gtgcagatga aagcctggac gcgcatgaat accagagcgt ctttgccaaa 540 cacatgggtg cggttgctgc tccgaccgcg tccctgcact tttctcagaa taccttggag 600 aagctgctta aggactttaa gcacgccttc ctgaccctgc acgttggcgc gggtacgttc 660 ctgagcgtgg aaaccaagga catccgcgaa catcagattc acaccgaagt gctacgtatt 720 ccgaagaaat cgcaagagat cctgcagaaa tcgcaagaaa tcctgtgtgt tggtacgact 780 gcgctccgct ccgtcgagta cttcaagcgc ctggagaacc cgaatcaaga ggcattcgag 840 tgcgatattt tcctgcactt cgccaacccg attctgcacg ttaattactt attgaccaat 900 tttcatctgc cgaagtccag cctgttaatg ctggtgagca ccatgatcgg cctggagaag 960 accaaagaaa tttataaaac ggcgatcgaa aagaagtatc gtttctacag ctacggcgac 1020 ggcatgctga tactg 1035 <210> 80 <211> 1038 <212> DNA <213> Artificial Sequence <400> 80 atgaaaacat ataatgttgc tatagtagga gcgtctggcg ctgtgggtca agaactgatt 60 aagggcctgg aaaacagctt tttcccaatc aagaagtttg taccgcttgc ctcgacgcgt 120 tcggctggca aaaaaatcaa ggcgttcaac aaggactacg aaattctgga aaccacccat 180 gaagtgtttg aacgtgaaaa aatcgacatc gccttcttca gcgctggtgg tagcgtctct 240 gaggagttcg caacttctgc atccaaaacc gcgctggtgg tcgataatac cagcttcttt 300 cgtttaaata aggacgtgcc gttggttgtg ccggaaatta acgccaaaga gatctttaat 360 gctccgttga acattatcgc taatccgaat tgttctacca ttcaaatgac ccagattctt 420 aacccgttgc acctgcactt taagattaaa agcgtcatcg tgtctaccta tcaggcggtg 480 tccggtgcag gcaataaagg tattgaaagc ctgaagaacg aactcaagac cgcattagag 540 tgcctggaga aggacccaac gatcgatttg aatcaggttc tgcaagcggg cgcgtttgcg 600 tacccgatcg cgttcaacgc gattgcgcat attgacacct ttaaaagaa cggctatacc 660 aaagaggagt tgaagatgct gcacgaaact cacaaaatca tgggtgttga ttttccgatc 720 agcgcaacgt gcgttcgtgt tcctgttctg cgcagccact ccgagagcct gagcattgcg 780 ttcgagaaag agttcgacct gaaggaggtt tatgaggttc tcaagaacgc gccgtccgtt 840 gccgtttgtg atgatccgag ccacaacctg tacccgaccc cgttgaaggc atcccatacc 900 gatagcgtgt tcatcggccg tctgcgcaaa gacctgtttg acaaaaaaac cctgcatggt 960 ttctgcgtgg ccgatcagct gagagtgggt gcggcaacga acgcgctgaa gatcgcgttg 1020 cactacatca aaaacgcg 1038 <210> 81 <211> 1038 <212> DNA <213> Artificial Sequence <400> 81 atggacttca taggatttga agatctaaaa tgcaaagaca aggagaacag ccagaaagtt 60 tttgttattc gtaatgataa gctgggcgat tttatcttgg cgattccggc attgatcgcg 120 ttaaagcacg ccttccttga gaagggtaaa gaggtctatc tgggcgtcgt ggtgccgagc 180 tataccaccc ctattgcgct ggaatttccg ttcattgatg aagtcattat cgaagacaat 240 cacttgagtg cgactctgaa gtccaagccg atcgacgcgc tgatctttct gttttctaac 300 tttaaaaacg cgcgcctggc gttcagcctg cgtaagagca tcccgtatat cctggctccg 360 aaaaccaaaa tctacagctg gctgtaccag aaaagcgtgc gtcaatctcg ttcgttgtgc 420 ttgaagacgg agtatgaata caacctggat ctcattcatg ccttctgtaa agaccacaac 480 ctgccgaacg cacaactgaa gaaaatcgct tggaaactga aggacaaatc caaagagcgc 540 agcataatcg cgtcgaaact gaatgcggat gttggcctgc tgtggattgg cgttcatatg 600 cacagcggcg gtagctcgcc ggtgctgccg gcatcgcatt tcatcaaatt aatcgactgc 660 ctgcacaaca acttgtcttg tgaaattatt ctgatctgcg gtccgggtga aagaaaggct 720 accgaggaac tgctcaagaa gatcccgttt gcccatctgt acgataccag ccactccttg 780 gttgatttgg cgaaactgtg cgcaaatctc tctgtttaca ttggcaatgc cagcggtcca 840 ctgcatgtga atgcgttgtt cgataaccag tccattggtt tctatccgaa cgagctgagc 900 gcatccattg ctcgctggcg tccgttcaac gagcgttttc taggtattac gccaccgaac 960 ggcagcaatg acatgggtct gatcgacatc gagaaggaag gtgagaagat cgtgggcttc 1020 atcaactacc gtaaaatg 1038 <210> 82 <211> 1041 <212> DNA <213> Artificial Sequence <400> 82 atgatatttt caaaattaat tggactattc tctcacgata ttgcgatcga tctgggcacg 60 gctaatacca ttgtgctggt caagggccag ggtatcatta ttaacgagcc aagcattgtt 120 gctgtacgca tgggtttgtt tgattccaag gcgtacgaca ttctggccgt gggtagcgaa 180 gccaaagaga tgctgggcaa aaccccgaac agcatccgcg caatccgtcc gatgaaagac 240 ggcgttattg cggattatga catcaccgcg aagatgattc gttattttat cgagaaagtt 300 cataaacgta aaacctggat tcgtccgcgg atcatggtat gcgttccgta cggcctcacc 360 tctgttgaac gtaatgcggt gaaagagagc actctgagcg caggcgcgcg tgaagtgttc 420 ctgatcgagg aaccgatggc tgcggccatc ggtgcgggcc tcccggttaa ggaaccgcag 480 ggttcgttaa ttgtcgatat cggcggaggc actacggaaa tcggggtcat ctccttgggc 540 ggtttggtta ttagcaagag catccgcgtt gcaggtgaca aattggacca aagcattgtg 600 gagtacatcc gcaagaaatt caacttgtta atcggcgagc gcaccggtga agagatcaaa 660 atcgaaatcg gttgtgctat taagctggac ccgccgctga ccatggaagt gtccggtcgt 720 gatcaggtaa gtggtctgtt gcacaccatc gaactgtcca gcgacgatgt tttcgaggcg 780 atcaaggacc aagtgcgtga gatttctagc gcgctgcgta gcgttctgga agaggtgaaa 840 ccagatttgg cgaaggacat cgttcaaaac ggtgtggtgc tgaccggtgg tggcgcactg 900 attaagggtc tggacaagta cctgtctgat atggtgaagc tgcctgttta tgttggcgac 960 gaaccgctgt tggcggtcgc caagggtacg ggtgaggcga ttcaggatct ggaccttctg 1020 tcgagagtgg gttttagcga g 1041 <210> 83 <211> 1041 <212> DNA <213> Artificial Sequence <400> 83 atggcaatag acgaagataa acaaaaggct atctctttgg ccattaaaca aattgacaag 60 gtgttcggca agggcgctct ggtgcgccta ggcgataagc aagtggaaaa aattgactcg 120 atcagcacgg gtagcttggg tctggatctc gcgttgggca tcggcggtgt tccgaagggt 180 cgtattattg aaatttacgg tccggaaagt tccggtaaaa ccaccctctc gcttcacatc 240 atcgcggaat gtcagaaaaa tggaggtgtt tgcgcgttca tcgacgctga gcacgcgtta 300 gacgtgcatt acgcgaagcg cttaggtgtg gacaccgaaa atcttcttgt gagccagcct 360 gataccggtg agcaggcgct ggagattctg gaaactatta cccgtagcgg gggaatcgac 420 ctggtagttg tcgacagcgt tgcggcattg accccgaaag cggagatcga tggtgacatg 480 ggtgaccagc acgtgggtct gcaggcgcgt ctgatgagcc atgccctgcg taaaatcacc 540 ggtgtcctgc ataaaatgaa taccaccctg atttttatca accagattcg tatgaaaatc 600 ggtatgatgg gttatggtag cccggaaacc acgactggcg ggaacgcgct gaagttctat 660 gccagcgtta gaattgacat ccgccgtatt gcatctctga agcaaaacga acaacacatc 720 ggcaatcgtg caaaggccaa ggtcgtgaag aacaaagttg caccgccgtt tcgtgaagca 780 gagttcgata tcatgtttgg tgagggcatt tcaaaagagg gcgagatcat tgactacggc 840 gttaaactgg acatcgttga taagtccggc gcgtggctga gctatcagga taagaaactg 900 ggccagggtc gcgagaacgc taaagctctg ctgaaagaag ataaggcgtt ggcggatgag 960 atcacgctga agatcaaaga gtccattggc tccaacgagg aaataatgcc gctgccagat 1020 gaaccgctgg aggagatgga a 1041 <210> 84 <211> 1041 <212> DNA <213> Artificial Sequence <400> 84 atggaatttt gtgtactatt cggaggggct tcttttgagc atgaaatcag cattgtgagc 60 gcaattgccc ttaagggtgt gttgaaggac cgtattaaat acttcatttt tttggacgaa 120 aaccaccact tctacctcat cgaggagagc aacatgcatt ccaagtactt tgctcagatt 180 aaagagaaaa agctgccgcc tctgatcctg actcacaatg gtctgctgaa gaactccttt 240 ctgggcgcaa aaattatcga actgccactg gtcatcaacc tggttcatgg tggtgatggt 300 gaagatggca aactggcgag cctgctggag ttctaccgca tcgcgtttat tggtccgcgt 360 attgaggcga gcgttctgtc ctacaacaaa tacctgacca aactgtatgc gaaagattta 420 ggcgtgaaaa ccctggacca cgtgctatta aacgagaaga accgtgcgaa cgcgctggac 480 ctgatgaatt ttaatttccc gttcatcatc aagccgaaca acgcgggttc tagcctgggc 540 gttaatgttg ttaaggaaga aaaagagctc gtctatgcgc tggacggcgc tttcgagtac 600 agcaaagaag tgctgatcga gccgtttatc cagggtgtaa aagagtataa cttggctggc 660 tgcaagatca agaaggattt ttgttttagc tacgtggaag agccgaataa acaagaattt 720 ttggacttca agcaaaagta cttggacttc agccgtaata aagcaccgaa agccaatttg 780 tctaacgcgc tggaagagca gttgaaggag aacttcaaga aactttataa tgatttgttt 840 gacggcgcca ttatccgctg cgatttcttc gtcatcaaaa atgaagttta tttgaatgaa 900 attaacccga ttccgggttc gttggcaaac tatttgttcg atgacttcaa aacgaccctg 960 gaaaacctcg ctcaaagcct gccgaagacc ccgaaaatcc aaatcaagaa ctcgtatctg 1020 ctgcagattc agaaaaacaa g 1041 <210> 85 <211> 1044 <212> DNA <213> Artificial Sequence <400> 85 atgaaagctc aatatttctt ttggatacta ttcctgatcg gtttctattg gatgatttac 60 ctgtaccagg actttctcat ggatgcgctg atcgctggcc tgttatgcgt tggcttcttc 120 caggttaaag tttttctgga taaacgtttt ttcaacgttg tttctagttt tctgtgcgtg 180 ctgatacttg cgtccgtgct gattgtgcca ctgtacttca ttgtctataa aagctctaat 240 attattttcg aaattaactt tgagaaattt tcagccctta taaagtggct gaagggtatc 300 atcaccgaaa acctgagcca ttttccgacc atccacgacg gcgcgtccaa atttttggag 360 aacttcagcg cggcatctat caccggctat ttgctgaaga ttagcagcta tgttggtcgt 420 tatagcctga agctgattac tgacgcattg tttattctgg gtctgttgtt ttttttcttt 480 tactatggcg aacgttttta ccgctacttc ctgggcgtgc tgccgctggg gatgaaccaa 540 agcaaaaaga tcttcgagga agttgcgggt atttaagaa ttgtcttgct cacctctctg 600 atcacggtta ttttggaagg tgtggccttc ggcgtcatga tcgtgtggtt tggccatgat 660 ggttggagcc tcggcatcct gtacggtctg gcttccttgg ttccggctgt gggtggtgcg 720 ctgatctgga ttccgatcgc catttatgag ctgtaccacg gcaatgttaa tgaagcgatc 780 ttcatcgctt tgtactcgat cttattgatc tccgtattga ttgacagcgt cattaagccg 840 attctcattg tgttatcaa gaagcgcatc ttcaaaacca ccctgaagat caacgagatg 900 ctgatcttt tcagcatgat cgcgggtatt tcgcaatttg gcttctgggg tattatcgtg 960 ggtccgacga tcaccgcatt cttcatcgcg ctgctacgtc tgtacgagaa ctatttcatt 1020 cagaatgagc aaaaagcatg tgaa 1044

Claims

1. A Helicobacter pylori whole protein chip, characterized in that: It includes a substrate and 1631 Helicobacter pylori proteins connected thereto; The 1631 Helicobacter pylori proteins are shown in the following table: Among the 1631 Helicobacter pylori proteins, the nucleic acid sequences encoding 85 Helicobacter pylori proteins numbered NO.1000-1084 are shown in SEQ ID NO.1-85.

2. The Helicobacter pylori whole protein chip according to claim 1, characterized in that: The 1631 Helicobacter pylori proteins are from Helicobacter pylori 26695 and Helicobacter pylori J99 strains.

3. The Helicobacter pylori whole protein chip according to claim 1, characterized in that: The substrate is an optical grade epoxy substrate.

4. A method for preparing a Helicobacter pylori whole protein chip according to any one of claims 1 to 3, characterized in that: The following steps are involved: A. preparing recombinant expression vectors containing nucleic acid sequences encoding 1631 Helicobacter pylori proteins, and transforming them into Escherichia coli; B. High-throughput preparation and purification of various Helicobacter pylori proteins; C. The purified Helicobacter pylori proteins are prepared into sample solutions and added to the corresponding well plate positions, and then spotted on the substrate by a spotter to obtain a full protein chip.

5. The method for preparing a Helicobacter pylori whole protein chip according to claim 4, characterized in that: In step B, the high-throughput preparation step is as follows: after culturing the E. coli obtained in step A in LB medium, IPTG is added to the obtained bacterial solution for induction culture, the supernatant is removed by centrifugation, and the obtained strain is stored for future use.

6. The method for preparing a Helicobacter pylori whole protein chip according to claim 4, characterized in that: In step B, the purification step is: S1. After the strain prepared by high-throughput is frozen and thawed, a mixture formed by mixing Lysis buffer and pretreated Ni-NTA is added, and the mixture is mixed and incubated; S2. Eluting the incubation solution obtained after incubation to obtain purified Helicobacter pylori proteins.

7. A kit comprising the Helicobacter pylori whole protein chip according to any one of claims 1 to 3.

8. Use of the Helicobacter pylori whole protein chip according to any one of claims 1 to 3 or the kit according to claim 7 in screening Helicobacter pylori proteins that interact with small molecule compounds.

9. The use according to claim 8, characterized in that: The small molecule compounds include nucleotide molecules, antibiotics, and metabolites.

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