A Chinese medicine composition for improving sexual function and its preparation method and application

Abstract

The present invention relates to a Chinese medicine composition for improving sexual function, a preparation method and an application thereof, wherein the Chinese medicine composition is prepared from the following raw materials in parts by weight: 2-10 parts of maca, 1-8 parts of rose, 2-8 parts of chuanxiong, 1-7 parts of morinda officinalis, 1-8 parts of polygonatum, 1-9 parts of wolfberry and 1-7 parts of clove. The Chinese medicine composition of the present invention is formulated by dialectical compatibility according to the pathogenesis of male reproductive health, and plays the role of soothing the liver and regulating blood, regulating meridians and tendons, tonifying the kidney and strengthening yang, and strengthening the waist and knees.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine composition for improving sexual function, a preparation method and an application thereof. Background Art

[0002] According to the survey reports made by the World Health Organization and relevant medical and health institutions, in the 21st century, male diseases are increasing at a rate of 3% per year and have become the third largest disease threatening men's health. Men's health has become a public health issue of common concern at home and abroad. According to the "China Male Sexual Happiness Index Survey Report" released by the Chinese Medical Development Research Association in Beijing in 2015, 70% of Chinese men believe that their sexual happiness index is unsatisfactory. With the development of economic globalization and the rapid development of China's economy in recent years, the public's demand for health has gradually increased, and reproductive health is an important part of the health field. Especially for men who are busy with work and under heavy pressure, being plagued by male diseases is indeed a "male secret". Paying attention to men's health, especially reproductive health, has become a social need.

[0003] In order to alleviate the above problems, the prior art discloses a variety of western medicines and Chinese medicine compositions that can be used to improve sexual function. However, for a long time, the effect of western medicines in treating sexual dysfunction or sexual dysfunction has not been ideal, and long-term use of western medicines is prone to drug resistance and has significant toxic side effects. Chinese medicine compositions, such as Chinese patent application CN201010102298.9, discloses a Chinese medicine composition for improving and enhancing sexual function and treating sexual dysfunction, which is mainly processed from Morinda officinalis, processed Polygonum multiflorum, Eucommia ulmoides, Alpinia oxyphylla, Cuscuta australis, Astragalus membranaceus, Dioscorea batatas, Lycium barbarum, Astragalus complanatus, Carapace of turtle, Black sesame, Poria cocos, Dragon tooth, Polygala tenuifolia, Cyathula officinalis, Jujube, etc., and has the effect of improving male sexual function; for another example, Chinese patent application CN201711216015.9 discloses a Chinese medicine composition for improving male sexual function and resisting fatigue, which is mainly processed from oyster peptide, milk polypeptide, Polygonatum sibiricum powder, maca powder, black ginger powder, burdock root powder, mulberry powder, Schisandra chinensis powder, Lycium barbarum powder, Ebony plum powder, isomaltulose, sucralose, etc., and has the effect of improving male sexual function and resisting fatigue. The above-mentioned Chinese medicine composition mainly improves sexual function by nourishing the kidney and strengthening yang, replenishing qi and blood, and consolidating essence and stopping diarrhea, but the components are complex, the cost is high, and there are no efficacy research reports, and the actual effect is unknown. Summary of the invention

[0004] In order to overcome the defects of the prior art such as poor effect on improving sexual function and high cost, and to make up for the deficiencies of the treatment principles and compatibility of the prior art, the present invention provides a traditional Chinese medicine composition for improving sexual function, and a preparation method and application thereof.

[0005] One object of the present invention is to provide a Chinese medicine composition for improving sexual function, which is formulated according to the syndrome differentiation and compatibility of the pathogenesis of male reproductive health, and plays the role of soothing the liver and regulating blood, regulating the meridians and tendons, tonifying the kidney and strengthening yang, and strengthening the waist and knees. Another object of the present invention is to provide a preparation method of the above-mentioned Chinese medicine composition for improving sexual function. Another object of the present invention is to provide a pharmaceutical use of the above-mentioned Chinese medicine composition for improving sexual function.

[0006] The above-mentioned object of the present invention is achieved by adopting the following technical scheme.

[0007] On the one hand, the present invention provides a traditional Chinese medicine composition for improving sexual function, which is prepared from the following raw materials in parts by weight: 2-10 parts of maca, 1-8 parts of rose, 2-8 parts of chuanxiong, 1-7 parts of morinda officinalis, 1-8 parts of polygonatum, 1-9 parts of wolfberry, and 1-7 parts of cloves.

[0008] Preferably, the Chinese medicine composition is prepared from the following raw materials in parts by weight: 2-8 parts of maca, 2-6 parts of rose, 2-6 parts of chuanxiong, 2-6 parts of Morinda officinalis, 3-6 parts of polygonatum, 2-7 parts of wolfberry, and 2-5 parts of clove.

[0009] More preferably, the Chinese medicine composition is prepared from the following raw materials in parts by weight: 4 parts of maca, 5 parts of rose, 5 parts of chuanxiong, 6 parts of Morinda officinalis, 6 parts of polygonatum, 7 parts of wolfberry, and 3 parts of cloves.

[0010] In the Chinese medicine composition of the present invention, maca is pungent, sweet and warm in nature, warms the kidney and strengthens yang, strengthens tendons and bones, dispels cold, relieves pain, regulates blood vessels, and has the effect of improving fertility and enhancing sexual desire. Rose, bitter in taste, warm in nature, can relieve liver, and can widen the stomach. Maca and rose are matched, and have the function of tonifying kidney yang and tonifying liver qi, and are the main medicine. Chuanxiong is pungent, warm, fragrant and dry, and can move without guarding. It can be dispersed, can reach the top of the head when going up, and can reach the blood when going down. It has a wide range of blood circulation and blood stasis removal effects, and is suitable for various diseases of blood stasis. Morinda officinalis is slightly warm in nature, tonifies kidney yang, strengthens tendons and bones, removes rheumatism, has enhanced anti-fatigue ability and anti-inflammatory effect, and is mainly used to treat impotence and spermatorrhea, rheumatic arthralgia, and tendon and bone weakness. Polygonatum sibiricum is mild in nature, replenishes qi and nourishes yin, strengthens the spleen, moistens the lungs, and benefits the kidneys. It is used for spleen and stomach qi deficiency, fatigue, insufficient stomach yin, insufficient essence and blood, soreness of the waist and knees, and premature graying of hair. Chuanxiong, Morinda officinalis, and Polygonatum sibiricum are used together as ministers to help the monarch medicine to invigorate yang and cure impotence. Lycium barbarum is mild in nature, nourishes the liver and kidneys, improves essence and improves eyesight, and is used for liver and kidney yin deficiency syndrome. This product is sweet and mild in nature, and has a nourishing and strengthening effect on the liver and kidneys. It can be used for all liver and kidney yin deficiency syndromes. Clove is pungent and warm in nature and flavor, warms the middle and descends the adverse, warms the kidney and assists yang, has the function of warming the kidney to invigorate yang and cure impotence, and can treat kidney deficiency impotence and uterine coldness. The above two herbs are adjuvants. The combination of various herbs is not limited to the traditional theory of kidney deficiency. While invigorating the kidney, it focuses on regulating the liver, and treats impotence with the main theory of dredging. "Health Preservation Prescription" and "Su Nu Jing" point out that the reason is that the front yin is closely related to the liver tendons, and the erection of the penis depends on liver blood. The whole formula is based on the core pathogenesis of erectile dysfunction, and works together to achieve the effect of "tonifying the kidney, soothing the liver, invigorating yang and curing impotence".

[0011] The components in the Chinese medicine composition of the present invention, namely maca, rose, chuanxiong, Morinda officinalis, polygonatum, wolfberry, and clove are active components, and other auxiliary ingredients can also be added to the present invention to prepare various required preparations. Preferably, the Chinese medicine composition includes a pharmaceutically acceptable carrier or excipient, and the Chinese medicine composition is a granule, tablet, capsule, extract, pill, powder, lozenge or oral solution.

[0012] In another aspect, the present invention provides a method for preparing the above-mentioned Chinese medicine composition. Specifically, the Chinese medicine composition of the present invention is usually prepared into wine, decoction, powder, etc.

[0013] When the Chinese medicine composition is a decoction, the medicinal materials can be directly weighed and decocted according to conventional methods.

[0014] Preferably, when the Chinese medicine composition is a decoction, after mixing the herbs, add 6-15 times of water, preferably 10 times of water, soak for 30 minutes, boil for 30-100 minutes, preferably 90 minutes, filter under reduced pressure, and use the filtrate for later use.

[0015] Preferably, rose and clove are added later, that is, 3-15 minutes before other drugs are decocted, preferably within 5 minutes.

[0016] When the Chinese medicine composition is in other dosage forms, it can be prepared by adding auxiliary materials and boiling in water, and the preparation method comprises the following steps:

[0017] (1) Take maca, rose, Chuanxiong, Morinda officinalis, Polygonatum sibiricum, wolfberry, and clove, grind and mix, add 8-12 times the volume of water, soak for 15-40 minutes, decoct for 50-150 minutes, filter under reduced pressure, set aside the filtrate, and retain the residue;

[0018] (2) adding 6-10 times the volume of water to the residue obtained in step (1), boiling for 50-90 minutes, filtering under reduced pressure, and setting aside the filtrate;

[0019] (3) combining the filtrates obtained in step (1) and step (2), passing through a 200-mesh sieve, and concentrating the filtrate to a relative density of 1.0-1.5; and adding auxiliary materials to prepare the traditional Chinese medicine composition.

[0020] Preferably, in step (1), the amount of water added is 10 times the volume, the soaking time is 30 minutes, and the decoction time is 90 minutes;

[0021] Preferably, in step (1), rose and clove are added within 5 minutes before other drugs are decocted.

[0022] Preferably, in step (2), the amount of water added is 8 times the volume, and the decoction time is 60 min;

[0023] Preferably, in step (3), the filtrate is concentrated to a relative density of 1.2;

[0024] Preferably, in step (3), when the traditional Chinese medicine composition is a powder, the auxiliary material is maltodextrin, and the powder is obtained after conventional spray drying.

[0025] Preferably, in step (3), when the Chinese medicine composition is an alcoholic liquor, the auxiliary material is a base liquor (a liquor containing about 50 v / v% ethanol), and the alcoholic liquor is obtained after blending and filtering.

[0026] In a specific embodiment, the preparation method comprises the following steps:

[0027] (1) Take maca, rose, Chuanxiong, Morinda officinalis, Polygonatum sibiricum, wolfberry, and clove, grind and mix, add 10 times the volume of water, soak for 30 minutes, decoct for 90 minutes, filter under reduced pressure, set aside the filtrate, and keep the residue;

[0028] (2) adding 8 times the volume of water to the residue obtained in step (1), boiling for 60 minutes, filtering under reduced pressure, and setting aside the filtrate;

[0029] (3) The filtrates obtained in step (1) and step (2) are combined, passed through a 200-mesh sieve, the filtrate is concentrated to a relative density of 1.2, maltodextrin is added, and the powder is obtained by spray drying to prepare a powdered Chinese medicine composition.

[0030] In another aspect, the present invention provides a use of the above-mentioned Chinese medicine composition in the preparation of a drug for improving sexual function, wherein the Chinese medicinal materials in the Chinese medicine composition of the present invention work together to exert their medicinal effects according to the principle of monarch, minister, assistant and envoy. The Chinese medicine composition can be taken in conventional doses, or taken together with other products for improving sexual function to achieve a more ideal effect.

[0031] Compared with the prior art, the raw materials of the Chinese medicine composition of the present invention are: maca, rose, chuanxiong, Morinda officinalis, polygonatum, wolfberry, clove, and the Chinese medicine composition improves sexual function by regulating the synergistic effect of the liver and kidney, and the formula is innovative. The efficacy research data of male rats shows that the Chinese medicine composition of the present invention can significantly shorten the male rats' back climbing latency and riding latency, and significantly increase the male rats' back climbing and riding times. DETAILED DESCRIPTION

[0032] The present invention will be described in detail below in conjunction with specific implementations and examples, and the advantages and various effects of the present invention will be more clearly presented. It should be understood by those skilled in the art that these specific implementations and examples are used to illustrate the present invention, rather than to limit the present invention.

[0033] Unless otherwise specified, various raw materials, reagents, instruments and equipment used in the present invention can be purchased from the market or prepared by existing methods.

[0034] Example 1 :Chinese medicine composition for improving sexual function

[0035] The recipe is as follows: 6g maca; 3g rose; 6g chuanxiong; 3g morinda officinalis; 3g polygonatum; 3g wolfberry; 3g clove.

[0036] The preparation method is as follows:

[0037] (1) Take maca, rose, Chuanxiong, Morinda officinalis, Polygonatum sibiricum, wolfberry, and clove, grind and mix, add 10 times the volume of water, soak for 30 minutes, decoct for 90 minutes (wherein, rose and clove are added within 5 minutes before the other drugs are decocted), filter under reduced pressure, set aside the filtrate, and retain the residue;

[0038] (2) adding 8 times the volume of water to the residue obtained in step (1), boiling for 60 minutes, filtering under reduced pressure, and setting aside the filtrate;

[0039] (3) The filtrates obtained in step (1) and step (2) are combined, passed through a 200-mesh sieve, and the filtrate is concentrated to a relative density of 1.2; maltodextrin is added, and the mixture is subjected to conventional spray drying to obtain a powder.

[0040] Embodiment 2: Traditional Chinese medicine composition for improving sexual function

[0041] The recipe is as follows: 100g maca; 80g rose; 80g chuanxiong; 70g morinda officinalis; 80g polygonatum; 90g wolfberry; 70g clove.

[0042] The preparation method is as follows:

[0043] (1) Take maca, rose, Chuanxiong, Morinda officinalis, Polygonatum sibiricum, wolfberry, and clove, grind and mix, add 10 times the volume of water, soak for 30 minutes, decoct for 90 minutes (wherein, rose and clove are added within 5 minutes before the other drugs are decocted), filter under reduced pressure, set aside the filtrate, and retain the residue;

[0044] (2) adding 8 times the volume of water to the residue obtained in step (1), boiling for 60 minutes, filtering under reduced pressure, and setting aside the filtrate;

[0045] (3) The filtrates obtained in step (1) and step (2) are combined, passed through a 200-mesh sieve, and the filtrate is concentrated to a relative density of 1.2; maltodextrin is added, and the mixture is subjected to conventional spray drying to obtain a powder.

[0046] Example 3 :Chinese medicine composition for improving sexual function

[0047] The recipe is as follows: maca 6g; rose 6g; chuanxiong 6g; morinda officinalis 6g; polygonatum 9g; wolfberry 6g; clove 6g.

[0048] The preparation method is as follows:

[0049] (1) Take maca, rose, Chuanxiong, Morinda officinalis, Polygonatum sibiricum, wolfberry, and clove, grind and mix, add 10 times the volume of water, soak for 30 minutes, decoct for 90 minutes (wherein, rose and clove are added within 5 minutes before the other drugs are decocted), filter under reduced pressure, set aside the filtrate, and retain the residue;

[0050] (2) adding 8 times the volume of water to the residue obtained in step (1), boiling for 60 minutes, filtering under reduced pressure, and setting aside the filtrate;

[0051] (3) The filtrates obtained in step (1) and step (2) are combined, passed through a 200-mesh sieve, and the filtrate is concentrated to a relative density of 1.2; maltodextrin is added, and the mixture is subjected to conventional spray drying to obtain a powder.

[0052] Embodiment 4:The formula of the Chinese medicine composition for improving sexual function is as follows: 40g of maca; 30g of rose; 30g of Chuanxiong; 30g of Morinda officinalis; 30g of Polygonatum sibiricum; 35g of wolfberry; and 25g of clove.

[0053] The preparation method is as follows:

[0054] (1) Take maca, rose, Chuanxiong, Morinda officinalis, Polygonatum sibiricum, wolfberry, and clove, grind and mix, add 10 times the volume of water, soak for 30 minutes, decoct for 9 minutes (wherein, rose and clove are added within 5 minutes before the other drugs are decocted), filter under reduced pressure, set aside the filtrate, and retain the residue;

[0055] (2) adding 8 times the volume of water to the residue obtained in step (1), boiling for 60 minutes, filtering under reduced pressure, and setting aside the filtrate;

[0056] (3) The filtrates obtained in step (1) and step (2) are combined, passed through a 200-mesh sieve, and the filtrate is concentrated to a relative density of 1.2; maltodextrin is added, and the mixture is subjected to conventional spray drying to obtain a powder.

[0057] Embodiment 5: Traditional Chinese medicine composition for improving sexual function

[0058] The recipe is as follows: 20g maca; 25g rose; 25g Chuanxiong; 30g Morinda officinalis; 30g Polygonatum sibiricum; 35g wolfberry; 15g clove.

[0059] The preparation method is as follows:

[0060] (1) Take maca, rose, Chuanxiong, Morinda officinalis, Polygonatum sibiricum, wolfberry, and clove, grind and mix, add 10 times the volume of water, soak for 30 minutes, decoct for 90 minutes (wherein, rose and clove are added within 5 minutes before the other drugs are decocted), filter under reduced pressure, set aside the filtrate, and retain the residue;

[0061] (2) adding 8 times the volume of water to the residue obtained in step (1), boiling for 60 minutes, filtering under reduced pressure, and setting aside the filtrate;

[0062] (3) The filtrates obtained in step (1) and step (2) are combined, passed through a 200-mesh sieve, and the filtrate is concentrated to a relative density of 1.2; maltodextrin is added, and the mixture is subjected to conventional spray drying to obtain a powder.

[0063] Experimental analysis

[0064] Experimental Example 1

[0065] 1. Experimental Materials

[0066] 1. Experimental animals: SD rats (SPF grade, 50 rats), male, 220-240 g.

[0067] 2. Test drugs: prepared from Examples 1-5, Haigou Pills (Shenzhen Jingguo Pharmaceutical Co., Ltd., 0.2 g / pill); estradiol benzoate injection (Tianjin Jinyao Pharmaceutical Co., Ltd., national medicine standard H12020530); progesterone injection (Zhejiang Xianju Pharmaceutical Co., Ltd., national medicine standard H33020828).

[0068] (II) Experimental methods

[0069] 1. Experimental groups: 70 male SD rats were randomly divided into control group, positive drug group, Example 1, Example 2, Example 3, Example 4, and Example 5 according to body weight, a total of 7 groups, with 10 animals in each group.

[0070] 2. Dosage design: The dosage of rats in each group of Example 1, Example 2, Example 3, Example 4 and Example 5 was 1.82g crude drug / kg body weight, 2.68g crude drug / kg body weight, 3.13g crude drug / kg body weight, 2.31g crude drug / kg body weight, and 1.89g crude drug / kg body weight. The control group was given an equal volume of normal saline, and the positive drug Haigou Pill was 0.26g / kg. Before administration, the drug was prepared into a corresponding concentration and then administered by gavage, and the administration volume was 0.1ml / 10g body weight. Gavage once a day for 30 consecutive days. Rats in each group were fed with ordinary feed and had free access to food and water.

[0071] 3. Methods for aphrodisiac in female mice

[0072] 48 hours before the experiment, female mice were subcutaneously injected with 10ug of estradiol (dissolved in 0.1ml of safflower oil); 2 hours before the experiment, progesterone was subcutaneously injected. Female mice showed lordosis when in estrus and bilateral ear flapping when in contact with male mice.

[0073] 4. Electrophysiological measurement of sexual activity

[0074] The rats were fixed in dorsal position, and the program of the biofunctional experimental system was set to the electrical stimulation mode. Stimulating electrodes were placed on the rat's penis and lower abdomen near the anus. Electrical stimulation was given and the erection latency and ejaculation latency of the rats were measured (the time from the start of stimulation to the erection of the penis was the erection latency; the time from the erection of the penis to the start of ejaculation was the ejaculation latency).

[0075] 5. Mating test

[0076] Place male rats and female rats in estrus together in cages, one pair per cage, and observe the sexual activity of male rats between 7 and 9 p.m. Place a male rat in a rat cage to acclimate for 10 minutes, then place a female rat in estrus in the cage, and immediately time and observe the sexual behavior of the male rat within 30 minutes. Record the following indicators: ① The time from the time the female rat is placed in the cage to the time the male rat first catches the female rat (back-climbing latency). ② The number of times the male rat climbs on the back within 30 minutes (back-climbing times). ③ The number of times the male rat licks the penis after successfully climbing on the back within 30 minutes (ejaculation times). ④ The time from the time the male rat successfully climbs on the back to the time it licks the penis (ejaculation latency).

[0077] (III) Experimental results

[0078] Effect of 30-day drug administration on sexual function of male rats: Compared with the control group, the five drug administration groups and the positive drug group showed significant changes in the back crawling latency, the number of back crawling, the number of ejaculation, the male rat erection latency and the ejaculation latency (P < 0.05); compared with the positive drug group, the back crawling latency, the number of back crawling, the number of ejaculation, the male rat erection latency and the ejaculation latency of the Example 5 group showed significant differences (P < 0.05); the comparison results showed that the effect of Example 5 was better. See Table 1 and Table 2 for details.

[0079] Table 1: Effects of each group of drugs on the latency period of back climbing, the number of back climbing and the number of ejaculations in rats

[0080]

[0081]

[0082] Note: Compared with the control group, *p<0.05; compared with the positive drug group, # p<0.05.

[0083] Table 2: Effects of each group of drugs on the erection latency and ejaculation latency of rats

[0084]

[0085] Note: Compared with the control group, *p<0.05; compared with the positive drug group, # p<0.05.

[0086] (IV) Experimental conclusion

[0087] In the experiment of sexual behavior of rats after 30 days of administration, it was observed that compared with the control group, the behavior of the positive drug group and the 5 examples was significantly improved, and the effect of Example 5 was the most obvious. Compared with the positive drug group, the mating data of rats after 30 days of administration showed that the latency of back climbing in the drug groups of Examples 1-5 was reduced, and the number of back climbing and sexual function indicators were also greatly improved, among which Example 5 had the better effect. As shown in Table 1 and Table 2, Example 5 can stimulate sexual desire and improve sexual function.

[0088] Experimental Example 2

[0089] 1. Experimental Materials

[0090] 1. Experimental animals: SD rats (SPF grade, 50 rats), male, 220-240 g.

[0091] 2. Test drugs: CN110547470A group, control group 1, (the formula is 100g zinc-rich and selenium-rich protein powder, 60g maca powder, 50g polygonatum, 40g wolfberry, 30g rose, 25g raspberry, 20g peach kernel, 20g oyster peptide, 20g burdock root, 10g L-carnitine, 10g ginseng, 5g cinnamon, 5g silicon dioxide, 3g xanthan gum, 0.05g vitamin A, 0.2g vitamin E, 1g vitamin C, 0.2g sucralose, 0.5g zinc gluconate, 0.5g taurine); CN108815378A, control group 2, (the formula is 16g polygonatum extract, 18g red deer antler, 16g epimedium extract, 25g bat moth Paecilomyces powder, polygonatum extract 8g); drugs of the present invention: (the formula of Examples 1 and 5).

[0092] Add 8 times the amount of water above, soak for 30 minutes, boil for 60 minutes, filter under reduced pressure, and keep the filtrate for later use.

[0093] (II) Experimental methods

[0094] 1. Experimental groups: 60 male SD rats were randomly divided into control group, positive drug group, CN110547470A group (comparison group 1), CN108815378A group (comparison group 2), Example 1 and Example 5, a total of 6 groups, each with 10 animals.

[0095] 2. Dosage design: The dosage of rats in Example 1 and Example 5 was 1.82g crude drug / kg body weight and 1.89g crude drug / kg body weight, respectively. Before administration, the drug was prepared into the corresponding concentration and then administered by gavage, and the administration volume was 0.1ml / 10g body weight. The control was gavaged with an equal volume of normal saline, the positive drug Haigou Pill was 0.26g / kg, the CN108815378A group was administered with 2g crude drug / kg per day, and the CN110547470A group was administered with 2.65g / kg, once a day, for 30 days.

[0096] 3. Methods for aphrodisiac of female mice, electrophysiological determination of sexual activity, and mating test: as in Experimental Example 1.

[0097] (III) Experimental results

[0098] Comparison of sexual behavior of male mice in each group: 30 days after administration, compared with the control group, the four drug groups and the positive drug group had changes in the back crawling latency, the number of back crawling, the erection latency, the ejaculation latency and the number of ejaculation, among which Example 5 had the most significant effect (P < 0.05); compared with the positive drug group, among the four drug groups, the back crawling latency, the erection latency and the ejaculation latency of the drug Example 5 of the present invention had significant differences (P < 0.05); the number of back crawling and the number of ejaculation increased significantly (P < 0.05); the comparison results showed that Example 5 of the present invention had a better effect. See Table 3 and Table 4 for details.

[0099] Table 3: Effects of each group of drugs on the latency period of back climbing, the number of back climbing and the number of ejaculations in rats

[0100]

[0101] Note: Compared with the control group, *p<0.05; compared with the positive drug group, # p<0.05.

[0102] Table 4: Effects of each group of drugs on the erection latency and ejaculation latency of rats

[0103]

[0104] Note: Compared with the control group, *p<0.05; compared with the positive drug group, # p<0.05.

[0105] (IV) Experimental conclusion

[0106] In the rat sexual behavior experiment, it was observed that compared with the control group, the sexual behavior of the positive drug group and the four drug groups was significantly improved, and the effect of Example 5 of the present invention was the most significant. Comparative analysis with the positive drug group showed that the mating data of rats after 30 days of administration showed that the back crawling latency and erection latency of Example 5 were significantly reduced, and the sexual function index was greatly improved, indicating that the drug group of the present invention can stimulate sexual desire and improve sexual function.

[0107] Experimental Example 3

[0108] 1. Experimental Materials

[0109] 1. Experimental animals: SD rats (SPF grade, 50 rats), male, 220-240 g.

[0110] 2. Test drug:

[0111] Group A: maca 20g, angelica 25g, chuanxiong 25g, morinda officinalis 30g, polygonatum 30g, wolfberry 35g, clove 15g;

[0112] Group B: 20g of pilose antler, 25g of rose, 25g of chuanxiong, 30g of morinda officinalis, 30g of polygonatum, 35g of wolfberry, and 15g of clove;

[0113] Group C: maca 20g, rose 25g, chuanxiong 25g, morinda officinalis 30g, dendrobium 30g, wolfberry 35g, clove 15g;

[0114] Group D: maca 20g, rose 25g, chuanxiong 25g, morinda officinalis 30g, polygonatum 30g, wolfberry 35g, clove 15g;

[0115] For each of the above drug groups, add 10 times the volume of water, soak for 30 minutes, boil for 90 minutes, filter under reduced pressure, and set aside the filtrate. Add 8 times the volume of water to the filter residue, boil for 60 minutes, and combine the two filtrates for later use.

[0116] (II) Experimental methods

[0117] 1. Experimental groups: 60 male SD rats were randomly divided into 6 groups according to body weight, namely control group, positive drug group, group A, group B, group C, and group D (Example 5 of the present invention), with 10 animals in each group.

[0118] 2. Dosage design: The dosage of rats in groups A, B, C and D was 1.89g crude drug / kg body weight. Before administration, the drug was prepared into corresponding concentrations and then administered by gavage, with a dosage volume of 0.1ml / 10g body weight. The control group was given an equal volume of normal saline and 0.26g / kg of the positive drug Haigou Pills, which were administered by gavage once a day for 30 consecutive days. Rats in each group were fed with ordinary feed and had free access to food and water.

[0119] 3. Methods for aphrodisiac of female mice, electrophysiological determination of sexual activity, and mating test: as in Experimental Example 1.

[0120] (III) Experimental results

[0121] 1. Comparison of sexual behavior of male mice in each group

[0122] After 30 days of administration, compared with the control group, the four drug groups and the positive drug group had significant changes in the back crawling latency, back crawling times, erection latency, ejaculation latency and ejaculation times (P < 0.05); compared with the positive drug group, among the present invention's group A, group B, group C and group D, the above sexual function indicators of group D were significantly improved (P < 0.05), and the effect of group D was better than that of the positive drug group. It can be seen that the effect of group D of the present invention is better. See Table 5 and Table 6 for details.

[0123] Table 5: Effects of each group of drugs on the latency period of back climbing, the number of back climbing and the number of ejaculations in rats

[0124]

[0125] Note: Compared with the control group, *p<0.05; compared with the positive drug group, # p<0.05.

[0126] Table 6: Effects of each group of drugs on the erection latency and ejaculation latency of rats

[0127]

[0128]

[0129] Note: Compared with the control group, *p<0.05; compared with the positive drug group, # p<0.05.

[0130] (IV) Experimental conclusion

[0131] In the experiment of sexual behavior activity of rats, it was observed that compared with the control group, the sexual behavior activity of the positive drug group and the four drug administration groups was significantly improved, and the effect of the D group of the present invention was the most obvious. Compared with the positive drug group, the mating data of rats after 30 days of administration showed that the back crawling latency and erection latency of the D group of the present invention were significantly reduced, the number of back crawling, ejaculation latency and ejaculation number were significantly increased, and the sexual function was improved to a certain extent. The D group of the present invention had better sexual function effects than the control group and the positive drug group, so it shows that the D group of the present invention is the best.

[0132] Experimental Example 4

[0133] 1. Experimental Materials

[0134] 1. Experimental animals: Male SD rats (SPF grade, 60 rats).

[0135] 2. Test drug:

[0136] Group A: 20g of maca, 25g of rose (added within 5 minutes before other drugs are decocted), 25g of Chuanxiong, 30g of Morinda officinalis, 30g of Polygonatum sibiricum, 35g of Lycium barbarum, 15g of clove (added within 5 minutes before other drugs are decocted), add 8 times of water, soak for 30min, decoct for 90min, filter under reduced pressure, and reserve the filtrate; add 8 times of water volume to the residue and decoct for 60min. The obtained filtrates are combined, passed through a 200-mesh sieve, and the filtrate is concentrated to a relative density of 1.2; add auxiliary materials to prepare the Chinese medicine composition.

[0137] Group B: 20g of maca, 25g of rose, 25g of Chuanxiong, 30g of Morinda officinalis, 30g of Polygonatum sibiricum, 35g of Lycium barbarum, and 15g of cloves, add 8 times of water, soak for 30min, decoct for 90min, filter under reduced pressure, and reserve the filtrate; add 8 times of water volume to the residue, decoct for 60min. Combine the obtained filtrates, pass through a 200-mesh sieve, and concentrate the filtrate to a relative density of 1.2; add auxiliary materials to prepare the traditional Chinese medicine composition.

[0138] (II) Experimental methods

[0139] 1. Experimental groups: 30 male SD rats were randomly divided into 3 groups according to body weight: control group, group A (Example 5 of the present invention), and group B, with 10 animals in each group.

[0140] 2. Dosage design: The dosage of rats in Group A (Example 5 of the present invention) and Group B was 1.89 g crude drug / kg body weight. Before administration, the drug was prepared into a corresponding concentration and then administered by gavage, and the administration volume was 0.1 ml / 10 g body weight. The control group was given an equal volume of normal saline, gavage once a day for 30 consecutive days. Rats in each group were fed with ordinary feed and had free access to food and water.

[0141] 3. Methods for aphrodisiac of female mice, electrophysiological determination of sexual activity, and mating test: as in Experimental Example 1.

[0142] (III) Experimental results

[0143] Comparison of sexual behavior of male mice in each group After 30 days of drug administration, compared with the control group, the two drug administration groups had significant changes in the back crawling latency, back crawling times, erection latency, ejaculation latency and ejaculation times (P < 0.05); compared with the positive drug group, the above sexual function indicators of group A and group B of the present invention were significantly different (P < 0.05); and the effect of group A of the present invention was better. See Table 7 and Table 8 for details.

[0144] Table 7: Effects of each group of drugs on the latency period of back climbing, the number of back climbing and the number of ejaculations in rats

[0145]

[0146] Note: Compared with the control group, *p<0.05; compared with the positive drug group, # p<0.05; A compared with B, $ p<0.05.

[0147] Table 8: Effects of each group of drugs on the erection latency and ejaculation latency of rats

[0148]

[0149] Note: Compared with the control group, *p<0.05; compared with the positive drug group, # p<0.05; A compared with B, $ p<0.05.

[0150] (IV) Experimental conclusion

[0151] In the rat sexual behavior experiment, it was observed that compared with the control group, the sexual behavior of the two drug groups was significantly improved, and the effect of the present invention group A was the most obvious. The rat mating data after 30 days of administration showed that the back crawling latency and erection latency of the present invention group A were significantly reduced, the number of back crawling, ejaculation latency and ejaculation times were significantly increased, and the sexual function indexes were greatly improved, indicating that the effect of the drug administration sequence of the present invention group A is better than that of the group B, which can stimulate the sexual desire of rats and improve sexual function better.

Claims

1. A Chinese medicine composition for improving sexual function, characterized in that: The Chinese medicine composition is prepared from the following raw materials in parts by weight: 4 parts of maca, 5 parts of rose, 5 parts of chuanxiong, 6 parts of Morinda officinalis, 6 parts of polygonatum, 7 parts of wolfberry and 3 parts of clove.

2. The Chinese medicine composition according to claim 1, characterized in that: The Chinese medicine composition comprises a pharmaceutically acceptable carrier or excipient, and the Chinese medicine composition is in the form of granules, tablets, capsules, extracts, pills, powders, lozenges or oral liquids.

3. Use of the Chinese medicine composition according to claim 1 or 2 in the preparation of a medicament for improving sexual function.

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