Quantitative blood sampling device and blood sampling method

Abstract

The present invention discloses a simple dry blood tip quantitative blood sampling device, comprising: a hydrophilic fiber material, a tip, a pipette and a tip box; the hydrophilic fiber material is loaded in the tip 0.8 to 2.0 cm away from the tip's liquid aspiration end. The present invention further analyzes the dry blood tip sample to verify that the present invention has the ability to quantitatively collect and analyze. The blood sampling device of the present invention integrates online collection, storage, transportation and processing, reduces the risk of sample contamination, and improves the accuracy of the quantitative detection results of the dry blood tip; at the same time, it is easy to operate, and patients can complete self-sampling without going to the hospital for blood collection, which can reduce the gathering of people and the occurrence of infectious diseases.

Description

Technical Field

[0001] The invention belongs to the field of medical examination and relates to a quantitative blood sampling device and a blood sampling method. Background Art

[0002] The dried blood spot (DBS) method is a simple blood sample collection technology that uses filter paper cards as carriers for trace blood samples. Compared with traditional sampling methods, the DBS method has the following advantages: DBS samples a small amount and does not require the involvement of professional medical staff, subjects can take samples at home, and the sampling process is less painful, and the risk of pathogen infection is also lower. In addition, DBS samples are samples that can be transported and stored at ambient room temperature, and DBS is a harmless substance that allows transportation by post. These factors make DBS sampling an ideal sample collection procedure for field use, especially in scenarios where it is difficult to use centrifuges to extract plasma, freezers, and dry ice to store and transport blood samples.

[0003] However, the traditional DBS method also has some shortcomings in quantitative sampling and analysis. These shortcomings mainly involve the amount of blood on the card, the hematocrit (HCT) of the blood, and the chromatographic effect of the filter paper. The traditional DBS method is to directly drop blood from the fingertip onto the filter paper card, and the blood drop will have volume differences and there is the possibility of repeated blood drops. On the other hand, the volume of blood used for analysis in the fixed-diameter sub-punch must be constant, and there must be a stable relationship between the blood volume applied to the card and the spot area it produces. In a very small volume range, the above relationship is valid, but as the volume increases, the existing relationship begins to break down. In addition, since low-HCT blood has a lower viscosity than high-HCT blood, the volume of low-HCT blood is smaller when a fixed-diameter punch is taken from a low-HCT spot than when the same-sized punch is taken from a high-HCT spot. The chromatographic effect of the filter paper refers to the fact that the diffusion rates of plasma and red blood cells in the blood are different in the filter paper, which may cause the analyte to be unevenly distributed in the dried blood spot when the target analyte binds to it. Therefore, the DBS method has poor quantitative analysis capabilities and is only suitable for some inspection items with low quantitative requirements. Its scope of application is limited to a certain extent.

[0004] The traditional DBS method requires continuous punching with a dedicated puncher, which is time-consuming and labor-intensive, and can easily cause cross-contamination between samples, reducing the accuracy of the test results. In addition, the frequent transfer of filter paper during sample collection also increases the risk of sample contamination. Summary of the invention

[0005] In order to overcome the shortcomings of the prior art, one of the purposes of the present invention is to provide a quantitative blood sampling device, which can be used for blood sampling by subjects at any location. The collected sample blood can be directly transferred to a tip box for drying, which is convenient for storage, transportation and sample processing.

[0006] The second object of the present invention is to provide a quantitative blood sampling method, which can achieve accurate volume blood sampling, has no restrictions on usage scenarios, and is easy to operate.

[0007] One of the purposes of the present invention is achieved by the following technical solution:

[0008] A quantitative blood sampling device comprises a suction tip, a pipette and a suction tip box. The suction tip is also provided with a hydrophilic fiber material for loading sample blood during blood sampling.

[0009] Furthermore, the hydrophilic fiber material is a natural fiber material and / or a synthetic hydrophilic fiber material and / or a composite hydrophilic fiber material.

[0010] Furthermore, the natural fiber material is cotton, linen, silk or wool; the synthetic hydrophilic fiber is hydrophilically modified polymer fiber and / or flocked fluff fiber; and the composite hydrophilic fiber material is a composite fiber obtained by blending two or more of the above materials.

[0011] Furthermore, preferably, the flocking hair is viscose fleece, nylon fleece, imported fleece, acrylic fleece or cotton fleece; and the hydrophilically modified polymer fiber is hydrophilically modified polyurethane fiber, hydrophilically modified polylactic acid fiber or hydrophilically modified polypropylene fiber.

[0012] Furthermore, preferably, the cotton fiber is absorbent cotton.

[0013] Furthermore, the pipette tip is a tube with two ends open, one end of the pipette tip is a liquid aspiration end for aspirating sample blood into the pipette tip; the other end of the pipette tip is a connecting end for detachably connecting with the pipette.

[0014] Furthermore, the hydrophilic fiber material is arranged near the liquid absorbing end of the suction head.

[0015] Furthermore, a tray with pipette tip holes is provided in the pipette tip box, and the pipette tip holes are used to fix the pipette tips; and the pipette is a pipette with adjustable range or a pipette with fixed range.

[0016] The second object of the present invention is achieved by adopting the following technical solution:

[0017] A quantitative blood sampling method is disclosed, wherein the method uses the quantitative blood sampling device obtained by the above technical solution to perform blood sampling.

[0018] Furthermore, the quantitative blood sampling method comprises the following steps:

[0019] (1) loading the hydrophilic fiber material into the pipette tip near the liquid aspiration end, then assembling the connecting end of the pipette tip with the pipette, and setting the volume;

[0020] (2) holding the pipette used in step (1) to absorb the sample blood, so that the sample blood infiltrates the hydrophilic fiber material, thereby completing blood collection;

[0021] (3) Open the pipette tip box, and place the pipette tips containing the sample blood and the hydrophilic fiber material in the pipette tip box to dry.

[0022] Furthermore, when the dried sample blood is tested, a fixed amount of extractant is added to the dried pipette tip containing the sample blood and the hydrophilic fiber, and the pipette is used to extract by multiple pipetting, and the extract is collected for testing.

[0023] Compared with the prior art, the present invention has the following beneficial effects:

[0024] The present invention provides a simple dry blood tip quantitative blood sampling device, comprising: a hydrophilic fiber material, a tip, a pipette and a tip box; the hydrophilic fiber material is loaded in the tip at a distance of 0.8 to 2.0 cm from the tip liquid suction end, the pipette nozzle is tightly connected to the tip connection end, the tip box has an upper box cover and a lower box cover, the upper box cover and the lower box cover are connected by a bendable fold line, the front sides of the upper box cover and the lower box cover are provided with mutually buckled buckles and clamps, the tip box is provided with a tray with a tip opening, and the tray is tightly embedded in the lower box cover. The tip and the tip box are adapted in specifications. The present invention further analyzes the dry blood tip sample to verify that the present invention has the ability of quantitative collection and analysis. The present invention integrates online collection, storage, transportation and processing, reduces the risk of sample contamination, and improves the accuracy of the quantitative detection results of the dry blood tip; at the same time, it is easy to operate, and patients can complete self-sampling without going to the hospital for blood collection, which can reduce the gathering of people and the occurrence of infectious diseases. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 It is a structural schematic diagram of the simplified dry blood suction tip quantitative blood sampling device of the present invention;

[0026] Figure 2 for Figure 1 A schematic diagram of the structure of a simple dry blood suction head quantitative blood sampling device;

[0027] Figure 3 This is a flow chart of the sample collection and testing steps of the dried blood tip;

[0028] Figure 4 The effect of absorbent cotton as hydrophilic fiber material on the sampling volume;

[0029] Figure 5 The influence of HCT effect on the sampling volume when absorbent cotton is used as the hydrophilic fiber material;

[0030] Figure 6When absorbent cotton is used as the hydrophilic fiber material, the effect of the amount of absorbent cotton on the sampling volume;

[0031] Figure 7 When absorbent cotton is used as the hydrophilic fiber material, the effect of the packing density of absorbent cotton on the sampling volume;

[0032] Figure 8 When absorbent cotton is used as the hydrophilic fiber material, the influence of absorbent cotton from different sources on the sampling volume;

[0033] Fig. 9 The stability of the dried blood tip sample of fluoxetine drug was investigated when nylon flocking fibers were used as the hydrophilic fiber material;

[0034] Fig.10 The stability of the dry blood tip sample of metformin drug was investigated when the hydrophilic polyurethane composite fiber was used as the hydrophilic fiber material;

[0035] Fig.11 The drug-drug curve of fluoxetine when nylon flocking fibers are used as hydrophilic fiber materials;

[0036] Fig.12 The drug-dose curve of metformin when the hydrophilic polyurethane composite fiber is used as the hydrophilic fiber material.

[0037] In the figure: 1, button; 2, pipette nozzle; 3, hydrophilic fiber material; 4, pipette tip; 5, pipette tip box; 6, fold line; 7, buckle; 8, clamping head; 9, tray; 10, pipette tip hole. DETAILED DESCRIPTION

[0038] The present invention is further described below in conjunction with the accompanying drawings and specific implementation methods. It should be noted that, under the premise of no conflict, the various embodiments or technical features described below can be arbitrarily combined to form a new embodiment.

[0039] Example 1

[0040] A schematic diagram of the structure and a schematic diagram of the structure decomposition of a simple dry blood suction head quantitative blood sampling device in an embodiment of the present invention are shown in FIG. Figure 1 , 2As shown, the blood sampling device includes a suction tip 4, a pipette and a suction tip box 5. The suction tip 4 can be a tube with two ends open for transferring the sample into the suction tip. The hydrophilic fiber material 3 is loaded in the suction tip 4 near the lower port (i.e., the liquid suction end) of the suction tip 4, and the pipette nozzle 2 is tightly connected to the upper port (i.e., the connecting end) of the suction tip 4. The suction tip box 5 includes an upper box cover and a lower box cover, the upper box cover is connected to the lower box cover by a fold line 6, and the front sides of the upper box cover and the lower box cover are provided with a buckle 7 and a clamp 8 that are mutually buckled. A tray 9 with a suction tip hole 10 is provided in the suction tip box, and the tray 9 is tightly embedded in the lower box cover. The suction tip 4 is adapted to the specifications of the suction tip box 5, and the suction tip hole 10 is used for inserting and removing the suction tip 4, and for fixing the suction tip 4 when it is inserted.

[0041] The simple dry blood tip quantitative blood sampling device of the present invention is mainly used for the quantitative collection and preservation of peripheral blood. In addition, it can also be used for the collection and preservation of clinical samples, such as urine and venous blood. The simple dry blood tip quantitative blood sampling device of the present invention uses hydrophilic fiber material as the supporting material, which is safer and reduces the risk of sample contamination. When combined with the tip as a simple blood sampling device, the material is cheap and easy to obtain, safe and environmentally friendly. In addition, the simple dry blood tip quantitative blood sampling device of the present invention is easy to operate and has low professional requirements for the operator. The micropipette can be combined with the wide-mouth end of the tip (i.e., the upper port), and quantitative sampling is achieved by pressing the pipette button. After the sampling is completed, the pipette range is adjusted counterclockwise, and the blood will be transferred to the hydrophilic fiber material in the tip by the suction force of the hydrophilic fiber material and the air pressure. The tip is transferred and sealed in the tip box. When the traditional dry blood spot method is used for sampling, the blood is usually dripped directly onto the filter paper card, which can easily cause large differences in sample volume and the possibility of repeated blood dripping. The sample collection and detection process is as follows Figure 3 As shown in the schematic diagram.

[0042] The simplified dry blood tip quantitative blood sampling device of the present invention has an integrated operation process from sample collection, storage, transportation to processing, which reduces the possibility of sample exposure and greatly reduces the risk of sample contamination. At the same time, the tip holes on the tray in the tip box are tightly and individually arranged, which will not cause cross contamination between samples. In the traditional dry blood spot method, the filter paper after blood collection needs to be exposed and dried, and during the storage and transfer process, the exposure opportunity is increased, which is easy to cause sample contamination.

[0043] The simplified dry blood tip quantitative blood sampling device of the present invention can directly process all samples without the need for traditional dry blood spot punching operations, thus saving a large amount of manpower and material resources, reducing cross contamination between samples caused by punching, and improving the accuracy of test results.

[0044] In one embodiment, the specification of the pipette tip can be 200 μL, and the specification can also be changed as needed. The pipette tip is made of plastic material, including but not limited to polypropylene and polyethylene. The pipette tip is preferably colorless and transparent to facilitate observation of sampling conditions. The main function of the pipette tip is to support the hydrophilic fiber material and the consumables for quantitative sampling.

[0045] like Figure 1 and 2 In a specific embodiment shown, the hydrophilic fiber material 3 is a supporting medium for a trace amount of blood, and a certain amount of the hydrophilic fiber material is loaded in the pipette tip 4 near the narrow end of the pipette tip, and the distance from the narrow end of the pipette tip is determined by the sampling amount, and preferably the sampling amount is close to the hydrophilic fiber material but not in contact with it, to prevent the hydrophilic fiber material's ability to absorb liquid from causing inaccurate sampling. In an application scenario, the hydrophilic fiber material is cotton wool, the blood collection volume is 10 μL, and the cotton wool is loosely loaded between 0.8 and 1.2 cm from the narrow end of the pipette tip.

[0046] In one application scenario, the pipette is an adjustable micropipette with a range of 5 to 50 μL. The pipette nozzle is combined with the wide-mouth end of the tip, and quantitative sampling is achieved by pressing the pipette button. After sampling, the pipette range is adjusted counterclockwise, and the blood will be transferred to the hydrophilic fiber in the tip by the suction force of the absorbent cotton and the air pressure. Among them, the pipette can be a micropipette with an adjustable range or a fixed range, which can be rotated according to the specific usage scenario. The sampling volume can be set as needed, and the general sampling volume is 10 to 20 μL.

[0047] like Figure 1 and 2 As shown, in the simplified dry blood tip quantitative blood sampling device of the present invention, the tip box 5 includes an upper box cover and a lower box cover, the upper box cover is connected to the lower box cover by a bendable fold line 6, and the front sides of the upper box cover and the lower box cover are provided with a buckle 7 and a clamp 8 that are mutually buckled. The main function of the tip box is to isolate and store the container of the sample. A bagged desiccant can be placed inside the tip box, which is conducive to shortening the drying time of the blood sample, or a desiccant can be placed without affecting the test results.

[0048] Example 2

[0049] A quantitative blood sampling method, using the quantitative blood sampling device as shown in Example 1, comprises the following steps:

[0050] (1) Open the tip box, mark the sample information on the left side of the tip hole in the tip box, and take out the tip pre-loaded with hydrophilic fiber material at the corresponding position, wherein the hydrophilic fiber is absorbent cotton.

[0051] (2) Connect the micropipette to the wide-mouth end of the pipette tip and adjust to the set volume.

[0052] (3) Disinfect the finger used for blood collection with alcohol, use a disposable medical blood collection needle to pierce the disinfected finger, squeeze out a drop of blood from the puncture, discard the first drop of blood and wipe it off with gauze.

[0053] (4) Hold the pipette with the narrow end of the pipette tip close to the blood drop, press the pipette button, and then slowly release the button. After the sampling is completed, adjust the pipette range counterclockwise. The blood will be completely transferred to the hydrophilic fiber material by the suction force of the hydrophilic fiber material and the air pressure.

[0054] (5) Open the tip box, insert the blood collection tip into the tip hole of the tray, seal it and place it to dry at room temperature. After drying, place it in a suitable environment for storage or transportation. You can also take out the dried blood tip separately, seal it in a medical bag, mark it with the sample information, and mail it.

[0055] (6) When analyzing the sample, open the tip box, take out the dry blood tip, assemble the micropipette and the dry blood tip, take a certain amount of extractant into the centrifuge tube, and extract the analyte in the blood sample by aspirating back and forth with the pipette.

[0056] Example 3

[0057] The blood collection method of Example 2 can also be used to collect human venous whole blood samples. The human venous whole blood samples are loaded onto a blood sample collection device using a cotton wool-loaded pipette tip to examine the accuracy and precision of the sampling volume.

[0058] 3.1 Effect of absorbent cotton on sampling volume

[0059] 5 mg of absorbent cotton was accurately weighed and loaded into the pipette tip, and 5, 8, 10, 15, and 20 μL of whole blood were quantitatively drawn using a pipette, and the blood collection volume was calculated by gravimetric analysis. The method described in Example 2 was repeated three times for each group. In addition, a sampling device without absorbent cotton was set as a control group.

[0060] The experimental results are as follows Figure 4 As shown, compared with the control group, the addition of absorbent cotton does not affect the accuracy of the blood sampling method of the present invention, and the sampling precision is good.

[0061] 3.2 Effect of different HCT on sampling volume

[0062] Prepare 5 whole blood matrices with HCT values ​​of 20%, 30%, 40%, 50%, and 60%, accurately weigh 5 mg of cotton wool and load it inside the pipette tip, use a pipette to quantitatively draw 10 μL of whole blood with different HCT, and calculate the blood collection volume by gravimetric analysis. The blood collection device without cotton wool was set as the control group. Three repeated experiments were set for each group, and the experimental steps were all as described in Example 2.

[0063] The experimental results are as follows Figure 5As shown, compared with the control group, the addition of absorbent cotton does not affect the accuracy of whole blood sampling of different HCT contents in the present invention, and the influence of HCT effect on the quantitative sampling of the present invention is small and can be ignored.

[0064] 3.3 Effect of different amounts of absorbent cotton on sampling volume

[0065] 3, 5, 8, 10, 12, and 15 mg of absorbent cotton were accurately weighed and loaded into the inside of the pipette tip to prepare several of them. 10 μL of whole blood was quantitatively drawn using a pipette, and the blood collection volume was calculated by gravimetric analysis. Three replicate experiments were set for each group, and the experimental process was as described in Example 2.

[0066] The experimental results are as follows Figure 6 As shown, the packing density of the absorbent cotton has little effect on the quantitative sampling of the present invention.

[0067] 3.4 Effect of cotton wool from different sources on sampling volume

[0068] 5 mg of absorbent cotton produced by four different manufacturers were accurately weighed and loaded into the pipette tip, and 10 μL of whole blood was quantitatively drawn using a pipette, and the blood collection volume was calculated by gravimetric analysis. Three replicate experiments were set up for each group, and the experimental process was as described in Example 2.

[0069] The experimental results are as follows Figure 7 As shown, cotton wool from different sources has no significant effect on the quantitative sampling of the present invention. By examining the influence of the above factors on sampling accuracy and precision, it can be seen that the blood sampling device of the present invention and the blood sampling method using the device have a wide range of application scenarios.

[0070] Example 4

[0071] The whole blood of rats was used for the detection of blood drug concentration. The improvement over Example 2 was that for the detection of fluoxetine, nylon flocking fibers were used to load the pipette tip; for the detection of metformin, hydrophilic polyurethane composite fibers were used to load the pipette tip. The other steps were the same as in Example 2.

[0072] Sample collection:

[0073] (1) Collect blood samples into anticoagulant tubes according to the abdominal aorta blood sampling procedure.

[0074] (2) Hold the pipette and place the narrow end of the pipette tip in contact with the whole blood sample. Press the pipette button and then slowly release the button.

[0075] After sampling is completed, adjust the pipette range counterclockwise, and the blood will be transferred to the hydrophilic fiber by the suction force of the hydrophilic fiber and the air pressure.

[0076] (3) Open the tip box, insert the blood-collecting tip into the tip hole of the tray, seal it and place it at room temperature to dry. After drying, store or transport it in a suitable environment.

[0077] (4) When analyzing the sample, open the tip box, take out the dry blood tip, assemble the micropipette and the dry blood tip, take a certain amount of extractant into the centrifuge tube for extraction, and the extraction process is to use the pipette to suck back and forth to allow the extractant to extract the analyte in the sample blood.

[0078] Sample extraction:

[0079] a) adding an appropriate amount of an internal standard compound to a pure methanol solution to prepare an extract containing the internal standard;

[0080] b) Add 100 μL of an extract containing an internal standard, wherein the extract is methanol, to a centrifuge tube. Extract the analyte in the blood sample by pipetting back and forth, and centrifuge the extract at 4000 rpm at room temperature for 5 minutes.

[0081] c) Transfer the extract into a sampling vial and perform analysis.

[0082] When the present invention is used for quantitative detection, the drug standard stock solution needs to be added to whole blood to prepare calibration products of different concentrations. The method of the present invention is used to prepare a dry blood tip, and then sample extraction and injection analysis are performed to obtain a calibration curve.

[0083] The present invention uses liquid chromatography-tandem triple quadrupole mass spectrometry (LC-MS / MS) for detection, which has high specificity and sensitivity. Tables 1 and 2 below show the LC-MS calibration curves and correlation coefficients of the dried blood tip samples and non-blood spot samples of the two drugs fluoxetine and metformin, and Table 3 shows the intra-day and inter-day accuracy and precision of the dried blood tip samples of the two drugs fluoxetine and metformin, as well as Fig. 9 and Fig.10 The stability of the dried blood tip samples of fluoxetine and metformin at different temperatures (4° C., 25° C., and 40° C.) illustrates the reliability of the quantitative detection of the present invention.

[0084] Table 1 Calibration curves, correlation coefficients and detection limits of dry blood tip samples of fluoxetine and metformin

[0085]

[0086] Table 2 Calibration curves, correlation coefficients and detection limits of non-blood spot samples of fluoxetine and metformin

[0087]

[0088] Table 3 Intra-day and inter-day recoveries and coefficients of variation of dried blood tip samples of fluoxetine and metformin

[0089]

[0090]

[0091] The method uses whole blood samples from the abdominal aorta of rats, which are loaded onto a collection device for blood drug concentration detection and compared with non-blood spot results. The detection results of the method are highly accurate and the coefficient of variation of the samples is low.

[0092] Example 5

[0093] Collection of whole blood from rats for pharmacokinetic monitoring

[0094] This method uses rat orbital venous whole blood samples, which are loaded onto a collection device for pharmacokinetic monitoring and compared with non-blood spot results. The main difference from Example 4 is that flocking hair is used as a hydrophilic fiber. This method has the advantages of simple operation, small sampling volume, stable sample and easy storage. The operation steps are as follows:

[0095] Sample collection:

[0096] (1) First, according to the animal administration principle, rats were gavaged with drugs (fluoxetine dosage 5 mg / Kg, metformin dosage 100 mg / Kg). Whole blood was collected at different time points into anticoagulant tubes according to the rat orbital blood collection operation standard.

[0097] (2) Hold the pipette and place the narrow end of the pipette tip in contact with the whole blood sample. Press the pipette button and then slowly release the button. After sampling is completed, adjust the pipette range counterclockwise. The blood will be transferred to the flocking hair by the suction force of the flocking hair and the air pressure.

[0098] (3) Open the tip box, insert the blood-collecting tip into the tip hole of the tray, seal it and place it at room temperature to dry. After drying, store or transport it in a suitable environment.

[0099] (4) When analyzing the sample, open the tip box, take out the dried blood tip, assemble the micropipette and the dried blood tip, and take a certain amount of extraction agent into a centrifuge tube for extraction.

[0100] Sample extraction:

[0101] a) adding an appropriate amount of an internal standard compound to a pure methanol solution to prepare an extract containing the internal standard;

[0102] b) Add 100 μL of internal standard extraction solution into a centrifuge tube, extract the analytes in the blood sample by pipetting back and forth, and centrifuge the extraction solution at 4000 rpm at room temperature for 5 minutes.

[0103] c) Transfer the extract into a sampling vial and perform analysis.

[0104] Test drug 1: Fluoxetine

[0105] Control: 10 μL of whole blood was quantitatively drawn with a pipette and directly added into the methanol solution containing the internal standard, and vortexed for 15 seconds; the extract was centrifuged at 10,000 rpm at room temperature for 10 minutes.

[0106] Experimental results: Fig.11 The fluoxetine drug-dose curve of rat dried blood tip sample (A) and the fluoxetine drug-dose curve of rat non-blood spot sample (B) show that the results of this method are consistent with those of the non-blood spot method.

[0107] Test drug 2: Metformin

[0108] Control: 10 μL of whole blood was quantitatively drawn with a pipette and directly added into the methanol solution containing the internal standard, and vortexed for 15 seconds; the extract was centrifuged at 10,000 rpm at room temperature for 10 minutes.

[0109] Experimental results: Fig.12 As shown in the metformin drug-time curve of the rat dry blood tip sample (A) and the metformin drug-time curve of the rat non-blood spot sample (B), the results of this method are consistent with those of the non-blood spot method, verifying the accuracy of the sampling device of the present invention and the blood collection method based on the device.

[0110] The above experiments show that the present invention can achieve quantitative sampling, and the accuracy of the detection results is high.

[0111] The above-mentioned embodiments are only preferred embodiments of the present invention and cannot be used to limit the scope of protection of the present invention. Any non-substantial changes and substitutions made by technicians in this field on the basis of the present invention shall fall within the scope of protection required by the present invention.

Claims

1. A quantitative blood sampling device, comprising a tip, a pipette, and a tip box, characterized in that: The suction tip is also provided with a hydrophilic fiber material, which is used to load sample blood during blood collection; The quantitative blood sampling method using the quantitative blood sampling device is: (1) loading the hydrophilic fiber material into the pipette tip near the liquid aspiration end, then assembling the connecting end of the pipette tip with the pipette, and setting the volume; (2) holding the pipette used in step (1) to absorb the sample blood, so that the sample blood soaks the hydrophilic fiber material, thereby completing blood collection; (3) Open the pipette tip box, and place the pipette tips containing the sample blood and the hydrophilic fiber material in the pipette tip box for drying.

2. The quantitative blood sampling device according to claim 1, characterized in that: The hydrophilic fiber material is a natural hydrophilic fiber material, a synthetic hydrophilic fiber material and / or a composite hydrophilic fiber material.

3. The quantitative blood sampling device according to claim 2, characterized in that: The natural hydrophilic fiber material is cotton, linen, silk or wool; the synthetic hydrophilic fiber material is hydrophilically modified polymer fiber and / or flocked fluff fiber; the composite hydrophilic fiber material is a composite fiber obtained by blending two or more of the above materials.

4. The quantitative blood sampling device according to claim 3, characterized in that: The flocking hair is viscose fleece, nylon fleece, imported fleece, acrylic fleece or cotton fleece; the hydrophilically modified polymer fiber is hydrophilically modified polyurethane fiber, hydrophilically modified polylactic acid fiber or hydrophilically modified polypropylene fiber.

5. The quantitative blood sampling device according to claim 1, characterized in that: The pipette tip is a tube with two ends open, one end of the pipette tip is a liquid aspiration end, used to aspirate sample blood into the pipette tip; the other end of the pipette tip is a connecting end, which is used to be detachably connected to the pipette.

6. The quantitative blood sampling device according to claim 5, characterized in that: The hydrophilic fiber material is arranged near the liquid absorbing end of the suction head.

7. The quantitative blood sampling device according to claim 1, characterized in that: A tray with a pipette tip hole is arranged in the pipette tip box, and the pipette tip hole is used to fix the pipette tip; the pipette is a pipette with adjustable range or a pipette with fixed range.

8. The quantitative blood sampling device according to claim 1, characterized in that: When the dried sample blood is tested, a fixed amount of extractant is added to the dried pipette tip containing the sample blood and the hydrophilic fiber, and the pipette is used to extract by multiple pipetting, and the extract is collected for testing.

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