A composition containing hyaluronic acid and its application in preparing a product with repairing effect

Abstract

The present invention relates to the technical field of repair active ingredients and preparation of repair products, and specifically discloses a composition containing hyaluronic acid and its application in preparing products with repair effects. The hyaluronic acid-containing composition comprises hyaluronic acid and lavender extract; wherein the weight ratio of hyaluronic acid to lavender extract is 1:4-6. The composition formed by mixing lavender extract and hyaluronic acid in the present invention has a certain repair effect; in particular, the composition formed by mixing lavender extract obtained by extracting lavender ethanol extract with organic solvent and hyaluronic acid has a further enhanced repair effect; therefore, it has important application value to use it as an active ingredient for preparing skin care products, cosmetics or medicines with repair effects.

Description

Technical Field

[0001] The present invention relates to the technical field of repair active ingredients and repair product preparation, and in particular to a composition containing hyaluronic acid and its application in preparing products with repair effects. Background Art

[0002] The skin is the largest organ in the human body. For an adult, the skin area is about 7,600 square centimeters, accounting for 15% of the total body weight, and about 1 / 3 of the circulating blood is supplied to the skin. Since the skin is the first line of defense of the human body, it is easily damaged by wound-causing factors and forms wounds. Wound healing refers to the process of regeneration, repair, and reconstruction of local tissues after the skin is damaged by wound-causing factors and tissue loss occurs. The repair of skin wounds depends on its own repair ability on the one hand, and on the other hand, it also requires the cooperation of the repair product to achieve faster and better repair.

[0003] Hyaluronic acid is a disaccharide unit glycosaminoglycan composed of D-glucuronic acid and N-acetylglucosamine. The existing technology fills it into some acne scars, scars caused by trauma or surgery, so as to fill scars. However, it has no obvious effect on promoting the repair of the skin itself; it is easily absorbed and metabolized by the human body, and the maintenance time is relatively short.

[0004] Therefore, it is of great application value to develop a composition with good repairing effect by using hyaluronic acid as raw material and adding other active ingredients. Summary of the invention

[0005] In order to overcome at least one of the technical problems existing in the prior art, the present invention provides a composition containing hyaluronic acid.

[0006] The above technical problems to be solved by the present invention are achieved through the following technical solutions:

[0007] A hyaluronic acid-containing composition, comprising hyaluronic acid and lavender extract;

[0008] Among them, the weight ratio of hyaluronic acid and lavender extract is 1:4-6.

[0009] Preferably, the weight ratio of the hyaluronic acid to the lavender extract is 1:5.

[0010] Preferably, the lavender extract is prepared by the following method:

[0011] The dried whole lavender is extracted with ethanol, and after the extraction is completed, the extract is concentrated and dried to obtain the lavender ethanol extract, namely the lavender extract.

[0012] Preferably, the ethanol is an ethanol aqueous solution with a volume fraction of 60-80%.

[0013] Most preferably, the ethanol is an ethanol aqueous solution with a volume fraction of 75%.

[0014] The inventor surprisingly found in the experiment that the composition composed of the lavender extract obtained by extraction with 60-80% by volume ethanol aqueous solution and hyaluronic acid has a certain repair effect.

[0015] Preferably, the weight amount of ethanol is 8 to 15 times that of the dried whole lavender;

[0016] Most preferably, the amount of ethanol by weight is 12 times that of the dry whole lavender.

[0017] Preferably, the extraction is heating reflux extraction; the extraction time is 1 to 2 hours.

[0018] Preferably, the lavender ethanol extract is further extracted.

[0019] The specific extraction method is: adding lavender ethanol extract into water and stirring evenly to obtain a suspension, then adding an organic solvent for extraction; separating the organic solvent layer, concentrating and drying the organic solvent layer to obtain the lavender extract.

[0020] The inventors further discovered in their research that the repairing effect of the composition formed by mixing the lavender extract obtained by extracting the lavender ethanol extract with an organic solvent and hyaluronic acid was further enhanced.

[0021] Preferably, the weight amount of water is 20 to 30 times the weight of the lavender ethanol extract.

[0022] Preferably, the volume ratio of water to the organic solvent is 1-2:1-2.

[0023] Most preferably, the volume ratio of water to organic solvent is 1:1.

[0024] Preferably, the organic solvent is a mixed organic solvent.

[0025] Preferably, the mixed organic solvent consists of n-hexane, chloroform and ethyl acetate in a volume ratio of 2 to 4:1 to 2:1.

[0026] Most preferably, the mixed organic solvent consists of n-hexane, chloroform and ethyl acetate in a volume ratio of 3:2:1.

[0027] The inventor surprisingly found in the study that when the composition composed of lavender extract obtained by extraction with an organic solvent consisting of n-hexane, chloroform and ethyl acetate and mixed with hyaluronic acid is selected, its repair effect is greatly improved and has excellent repair effect; its repair effect is much higher than the composition composed of lavender extract obtained by extraction with n-hexane, chloroform or ethyl acetate alone and mixed with hyaluronic acid; it is also much higher than the composition composed of lavender extract obtained by extraction with any two of n-hexane, chloroform and ethyl acetate and mixed with hyaluronic acid. The lavender extract obtained by extraction with an organic solvent consisting of n-hexane, chloroform and ethyl acetate can synergistically improve the repair effect of the composition composed of lavender extract mixed with hyaluronic acid.

[0028] Preferably, the hyaluronic acid-containing composition further contains α-mangostin.

[0029] Preferably, the weight ratio of the hyaluronic acid to the lavender extract and α-mangostin is 1:4-6:1.

[0030] The inventors further surprisingly found in the study that α-mangostin has an excellent repairing effect; adding α-mangostin to the composition consisting of hyaluronic acid and lavender extract can further significantly improve the repairing effect of the composition consisting of hyaluronic acid and lavender extract.

[0031] At the same time, the inventors also found that the repair effect of the composition composed of hyaluronic acid, lavender extract and α-mangostin is also better than that of single α-mangostin and the composition composed of hyaluronic acid and lavender extract; this shows that adding α-mangostin to the composition composed of hyaluronic acid and lavender extract can synergistically improve the repair effect of the prepared composition.

[0032] The present invention also provides an application of the above-mentioned hyaluronic acid-containing composition in preparing a product with a repairing effect.

[0033] Preferably, the product is a skin care product, a cosmetic or a medicine.

[0034] Beneficial effects: The present invention provides a new composition comprising hyaluronic acid and lavender extract; studies have shown that the composition composed of lavender extract obtained by extraction with 60-80% ethanol aqueous solution by volume and hyaluronic acid has a certain repair effect; in particular, the composition composed of lavender extract obtained by extracting lavender ethanol extract by organic solvent and hyaluronic acid has a further enhanced repair effect; further, adding α-mangostin to the composition composed of hyaluronic acid and lavender extract can further significantly improve the repair effect of the composition composed of hyaluronic acid and lavender extract. Since the composition of the present invention has a good repair effect, it has important application value to use it as an active ingredient in the preparation of skin care products, cosmetics or drugs with repair effects. DETAILED DESCRIPTION

[0035] The present invention is further explained below with reference to the examples, but the examples do not limit the present invention in any form.

[0036] Example 1 Preparation of a composition containing hyaluronic acid

[0037] The hyaluronic acid-containing composition is prepared by mixing hyaluronic acid and lavender extract in a weight ratio of 1:5;

[0038] The lavender extract is prepared by the following method: the dried whole lavender is heated to boiling state with 12 times the weight of 75% ethanol aqueous solution and extracted for 1.5 hours. After the extraction, the extract is concentrated and dried to obtain the lavender ethanol extract, namely the lavender extract.

[0039] Example 2 Preparation of a composition containing hyaluronic acid

[0040] The hyaluronic acid-containing composition is prepared by mixing hyaluronic acid and lavender extract in a weight ratio of 1:4;

[0041] The lavender extract is prepared by the following method: the dried whole lavender is heated to boiling state with 15 times the weight of 60% ethanol aqueous solution and extracted for 2 hours. After the extraction, the extract is concentrated and dried to obtain the lavender ethanol extract, namely the lavender extract.

[0042] Example 3 Preparation of a composition containing hyaluronic acid

[0043] The hyaluronic acid-containing composition is prepared by mixing hyaluronic acid and lavender extract in a weight ratio of 1:6;

[0044] The lavender extract is prepared by the following method: the dried whole lavender is heated to boiling state with 80% ethanol aqueous solution with a volume fraction of 8 times the weight, and extracted for 1 hour. After the extraction, the extract is concentrated and dried to obtain the lavender ethanol extract, namely the lavender extract.

[0045] Example 4 Preparation of a composition containing hyaluronic acid

[0046] The hyaluronic acid-containing composition is prepared by mixing hyaluronic acid and lavender extract in a weight ratio of 1:5;

[0047] The lavender extract is prepared by the following method:

[0048] (1) extracting the dried whole lavender with 12 times the weight of a 75% ethanol aqueous solution by volume, heating it to boiling state for 1.5 hours, and concentrating and drying the extract after the extraction to obtain a lavender ethanol extract;

[0049] (2) adding lavender ethanol extract to 25 times the weight of water and stirring to obtain a suspension, and then adding an organic solvent for extraction; separating the organic solvent layer, and concentrating and drying the organic solvent layer to obtain the lavender extract;

[0050] Wherein, in step (2), the volume ratio of water to organic solvent is 1:1;

[0051] The organic solvent described in step (2) is n-hexane.

[0052] Example 5 Preparation of a composition containing hyaluronic acid

[0053] The difference between Example 5 and Example 4 is that the organic solvent used for extraction during the preparation of the lavender extract is different, and the rest is the same as Example 4;

[0054] The organic solvent in Example 5 is chloroform.

[0055] Example 6 Preparation of a composition containing hyaluronic acid

[0056] The difference between Example 6 and Example 4 is that the selection of the organic solvent for extraction during the preparation of the lavender extract is different, and the rest is the same as Example 4;

[0057] The organic solvent in Example 6 is ethyl acetate.

[0058] Example 7 Preparation of a composition containing hyaluronic acid

[0059] The difference between Example 7 and Example 4 is that the selection of the organic solvent for extraction during the preparation of the lavender extract is different, and the rest is the same as Example 4;

[0060] The organic solvent in Example 7 is composed of n-hexane and chloroform in a volume ratio of 3:2.

[0061] Example 8 Preparation of a composition containing hyaluronic acid

[0062] The difference between Example 8 and Example 4 is that the organic solvent used for extraction during the preparation of the lavender extract is different, and the rest is the same as Example 4;

[0063] The organic solvent in Example 8 is composed of n-hexane and ethyl acetate in a volume ratio of 3:1.

[0064] Example 9 Preparation of a composition containing hyaluronic acid

[0065] The difference between Example 9 and Example 4 is that the selection of the organic solvent for extraction during the preparation of the lavender extract is different, and the rest is the same as Example 4;

[0066] The organic solvent in Example 9 is composed of chloroform and ethyl acetate in a volume ratio of 2:1.

[0067] Example 10 Preparation of a composition containing hyaluronic acid

[0068] The difference between Example 10 and Example 4 is that the selection of the organic solvent for extraction during the preparation of the lavender extract is different, and the rest is the same as Example 4;

[0069] The organic solvent in Example 10 is composed of n-hexane, chloroform and ethyl acetate in a volume ratio of 3:2:1.

[0070] Example 11 Preparation of a composition containing hyaluronic acid

[0071] The hyaluronic acid-containing composition is prepared by mixing hyaluronic acid, lavender extract and α-mangostin in a weight ratio of 1:5:1;

[0072] The preparation method of the lavender extract is the same as that of Example 10.

[0073] Experimental example

[0074] 130 6-week-old female KM mice were randomly divided into 13 groups, 10 mice in each group after adaptive feeding for 1 week; 12 groups were experimental groups and 1 group was a control group. After grouping, the backs of the mice in each group were shaved, and the straight skin of the back (circle with a diameter of 1 cm) was cut off under anesthesia, and then the wound was sutured to prevent it from shrinking; then the experimental group applied vaseline containing 1% of the sample to be tested on the wound; the control group applied vaseline without the sample to be tested; the application was done once in the morning and evening every day; after 7 days, the new skin of the mice in each group was observed, and the wound healing rate was calculated; the test results are shown in Table 1.

[0075] Wound healing rate (%) = (1-unhealed wound area / original wound area) × 100%

[0076] The samples tested in experimental groups 1 to 12 were the hyaluronic acid-containing compositions prepared in Examples 1 to 11 and α-mangostin alone, respectively.

[0077] Table 1. Experimental study on the repair effect of the composition containing hyaluronic acid of the present invention

[0078] Group Test samples Wound healing rate (%) Control group - 14.4±1.8 Experimental Group 1 The composition containing hyaluronic acid described in Example 1 25.1±2.7 Experimental Group 2 The composition containing hyaluronic acid described in Example 2 23.9±2.2 Experimental Group 3 The composition containing hyaluronic acid described in Example 3 24.4±2.5 Experimental Group 4 The composition containing hyaluronic acid described in Example 4 29.7±3.8 Experimental Group 5 The composition containing hyaluronic acid described in Example 5 28.2±3.5 Experimental Group 6 The composition containing hyaluronic acid described in Example 6 27.1±3.2 Experimental Group 7 The composition containing hyaluronic acid described in Example 7 28.9±3.6 Experimental Group 8 The composition containing hyaluronic acid described in Example 8 27.7±3.4 Experimental Group 9 The composition containing hyaluronic acid described in Example 9 28.2±3.7 Experimental Group 10 The composition containing hyaluronic acid described in Example 10 41.9±5.5 Experimental Group 11 The composition containing hyaluronic acid described in Example 11 70.1±8.6 Experimental Group 12 α-Mangostin 61.3±7.1

[0079] From the experimental data in Table 1, it can be seen that the wound healing rate of experimental groups 1 to 3 after using the composition containing hyaluronic acid described in Examples 1 to 3 is significantly higher than that of the control group. This shows that the composition composed of the lavender extract obtained by extracting with an ethanol aqueous solution with a volume fraction of 60 to 80% and hyaluronic acid has a certain repair effect.

[0080] From the experimental data in Table 1, it can be seen that the wound healing rate of experimental groups 4 to 9 using the hyaluronic acid-containing composition described in Examples 4 to 9 is improved relative to the hyaluronic acid-containing composition described in Example 1, but the improvement is not particularly large. This shows that the repair effect of the composition composed of the lavender extract obtained by extracting the lavender ethanol extract with an organic solvent and hyaluronic acid is enhanced, but the enhancement is not particularly significant. From the experimental data in Table 1, it can be seen that after the experimental group 10 uses the hyaluronic acid-containing composition described in Example 10, the wound healing rate is greatly improved relative to the hyaluronic acid-containing composition described in Example 1; it is also much higher than the hyaluronic acid-containing composition described in Examples 4 to 9. This shows that only when the composition composed of the lavender extract obtained by extraction with an organic solvent composed of n-hexane, chloroform and ethyl acetate and hyaluronic acid is selected, its repair effect can be greatly improved.

[0081] In addition, it can be seen from the wound healing rates of experimental groups 4 to 10 that the repair effect of the composition composed of the lavender extract obtained by extraction with an organic solvent consisting of n-hexane, chloroform and ethyl acetate and mixed with hyaluronic acid in Example 10 is much higher than that of the composition composed of the lavender extract obtained by extraction with n-hexane, chloroform or ethyl acetate alone and mixed with hyaluronic acid; it is also much higher than the composition composed of the lavender extract obtained by extraction with a combination of any two of n-hexane, chloroform and ethyl acetate and mixed with hyaluronic acid; this also shows that the lavender extract obtained by extraction with an organic solvent consisting of n-hexane, chloroform and ethyl acetate can synergistically improve the repair effect of the composition composed of the lavender extract mixed with hyaluronic acid.

[0082] It can be seen from the experimental data in Table 1 that the wound healing rate of α-mangostin reaches 61.3%, which has a very excellent repair effect; when it is added to the composition composed of hyaluronic acid and lavender extract, the wound healing rate of the hyaluronic acid-containing composition described in Example 11 is further greatly improved compared with that in Example 10. This shows that adding α-mangostin to the composition composed of hyaluronic acid and lavender extract can further greatly improve the repair effect of the composition composed of hyaluronic acid and lavender extract; so that the hyaluronic acid-containing composition has a very excellent repair effect. In addition, after the experimental group 11 adopts the hyaluronic acid-containing composition described in Example 11, its wound healing rate is also higher than that of α-mangostin; this shows that adding α-mangostin to the composition composed of hyaluronic acid and lavender extract can synergistically improve the repair effect of the prepared composition.

Claims

1. A composition containing hyaluronic acid, characterized in that: Contains hyaluronic acid and lavender extract; Among them, the weight ratio of hyaluronic acid and lavender extract is 1:4~6; The lavender extract is prepared by the following method: The dried whole lavender herb is extracted with ethanol, and after the extraction is completed, the extract is concentrated and dried to obtain the lavender ethanol extract; The lavender ethanol extract is added to water and stirred to obtain a suspension, and then an organic solvent is added for extraction; the organic solvent layer is separated, and the organic solvent layer is concentrated and dried to obtain the lavender extract; The organic solvent is a mixed organic solvent; the mixed organic solvent is composed of n-hexane, chloroform and ethyl acetate in a volume ratio of 2-4:1-2:1; The ethanol used is an ethanol aqueous solution with a volume fraction of 60-80%; The weight of ethanol used is 8 to 15 times that of the dried whole lavender. The volume ratio of water to organic solvent is 1~2:1~2.

2. The hyaluronic acid-containing composition according to claim 1, characterized in that The weight ratio of the hyaluronic acid to the lavender extract is 1:

5.

3. The composition containing hyaluronic acid according to claim 1, characterized in that The ethanol used is an ethanol aqueous solution with a volume fraction of 75%.

4. The hyaluronic acid-containing composition according to claim 1, characterized in that The weight amount of ethanol used is 12 times that of the dry whole lavender.

5. The hyaluronic acid-containing composition according to claim 1, characterized in that The extraction is heating reflux extraction; the extraction time is 1 to 2 hours.

6. The hyaluronic acid-containing composition according to claim 1, characterized in that The weight amount of water is 20 to 30 times the weight of the lavender ethanol extract.

7. The hyaluronic acid-containing composition according to claim 1, characterized in that The volume ratio of water to organic solvent is 1:

1.

8. Use of the hyaluronic acid-containing composition according to any one of claims 1 to 7 in the preparation of a product with a repairing effect.