A cultivation method for improving the quality of Stropharia officinalis fruiting bodies

Abstract

This invention discloses a cultivation method for improving the quality of *Pleurotus ostreatus* fruiting bodies, comprising the following steps: 1) Preparation of fruiting culture medium: mixing 15%–35% cotton residue, 25%–35% sawdust, 15%–30% rice husks, 10%–30% corn cobs, and 1% lime, and setting aside; 2) Composting and fermentation; 3) Secondary fermentation in a greenhouse; 4) Sowing; 5) Mycelial growth; 6) Covering with soil; 7) Inducing fruiting bud formation; 8) Growing mushrooms: after fruiting buds form, controlling the air temperature in the mushroom house to drop to 13–15℃, while maintaining the medium temperature at 15–16℃, to cultivate fruiting body growth; 9) Harvesting promptly when the fruiting bodies reach 7–8 maturity and are 4.5–5.5 cm in height to obtain *Pleurotus ostreatus*. This scheme uses cotton residue and sawdust as mushroom cultivation materials. After the mushroom buds are formed, the air temperature in the mushroom house is controlled at around 13℃ and the material temperature at around 16℃, which greatly improves the quality of the fruiting bodies, thereby increasing the rate of high-quality mushrooms to 65%-71%. The rate of high-quality mushrooms is twice that of ordinary culture materials used in field cultivation. At the same time, it effectively utilizes cotton residue and sawdust, resulting in significant economic and ecological benefits.

Description

Technical Field

[0001] The invention relates to a cultivation technology of Stropharia rugosa, and in particular to a cultivation method for improving the quality of Stropharia rugosa fruiting bodies. Background Art

[0002] Stropharia is one of the special varieties recommended by the Food and Agriculture Organization of the United Nations for cultivation in developing countries. Fresh mushrooms are bright in color, tender in texture, fragrant and delicious, and rich in nutrition. Stropharia is an edible fungus that can be directly cultivated with fresh straw and other stalks. Agricultural waste can be used to grow Stropharia, and the waste after cultivation can be directly returned to the field to increase fertility and improve soil. Stropharia has strong resistance to stress, low production cost, no additives, pesticides, hormones in production, and high protein, low fat food. It is a pure natural and pollution-free green food and the best carrier of circular agriculture. Artificial cultivation generally grows on substrates such as straw, wheat straw, and corn straw. Due to the unstable cultivation yield and quality, the production of Stropharia in field cultivation is greatly limited. Therefore, in order to increase the cultivation volume of Stropharia, adapt to the needs of greenhouse cultivation and improve biological efficiency, it is of great significance to carry out technical research on improving the quality of fruiting bodies of Stropharia.

[0003] The key to improving the quality of the fruiting body of Pleurotus ostreatus is the cultivation material formula and the mushroom cultivation method. There are many methods to obtain the culture medium formula, such as rice straw culture medium formula, wheat straw culture medium formula, corn straw culture medium formula and combination method. The specific production process is: material preparation → culture medium preparation → fermentation → inoculation → cultivation. There are many existing patents for the relevant cultivation material formula and specific production process. For example, the patent application with publication number CN103588577A discloses a method for making Pleurotus ostreatus cultivation material using sugarcane leaves and leaf tips, and discloses a method for preparing cultivation material by culturing the raw materials composed of large sugarcane leaves and leaf tips; the patent application with publication number CN110235701A discloses a method for planting and cultivating Pleurotus ostreatus, and discloses a method for cultivating Pleurotus ostreatus in a field ridge using corn stalks and corn cobs as a cultivation base.

[0004] However, the above cultivation methods all have the following problems: 1. The germination rate of the fungus is low, and pests are very likely to occur after sowing; 2. The biological efficiency is low, especially the quality is unstable, and the rate of high-quality mushrooms is not high. Therefore, a better method for improving the quality of the fruiting body of Stropharia rugosa is needed. Summary of the invention

[0005] The invention aims to overcome the above-mentioned shortcomings and propose a cultivation method for improving the quality of the fruiting bodies of Pleurotus eryngii. The fruiting bodies of Pleurotus eryngii cultivated by the method have fewer insect pests, thick caps, large caps, long and thick stipes, large single mushroom weight and high quality mushroom rate.

[0006] The object of the present invention is achieved through the following technical solutions:

[0007] A cultivation method for improving the quality of Stropharia officinalis fruiting bodies comprises the following steps:

[0008] 1) Preparation of mushroom culture medium: Mix 15%-35% cotton residue, 25%-35% sawdust, 15%-30% rice husk, 10%-30% corn cob and 1% lime and set aside;

[0009] 2) Composting and fermenting: composting and fermenting the mushroom culture medium prepared in step 1) for later use;

[0010] 3) Secondary fermentation in greenhouse: the culture medium in step 2) is moved into the greenhouse for secondary fermentation. The total time of the secondary fermentation is 5 to 6 days. After the secondary fermentation is completed, the culture medium for fermentation of Stropharia rugosa is obtained.

[0011] 4) Sowing: Inoculate the Stropharia occidentalis cultivar into the culture medium prepared in step 3) by hole sowing, with a seeding rate of 0.5-0.7 kg / m 2 ;

[0012] 5) Incubation: Cover the fermentation culture medium of the Stropharia officinalis in step 4) with a film, maintain the temperature in the mushroom house at 20-25°C, the material temperature at 24-26°C, the relative humidity in the mushroom house at 78-82%, the CO2 concentration at 2500-3500 mg / kg, and the incubation time at 30-35 days;

[0013] 6) Covering with soil: Cover the fermentation culture medium of Stropharia officinalis in step 5) with soil with a thickness of 3 to 3.5 cm, maintain the temperature in the mushroom house at 20 to 25° C., and the temperature of the culture medium at 24 to 26° C. for 10 to 13 days;

[0014] 7) Bud formation: Control the material temperature to drop to 19℃ at an average rate of 1℃ per day, and the temperature in the mushroom house to around 16-18℃. Increase fresh air to evenly reduce the CO2 concentration in the mushroom house every day, and finally drop it to 1000-1500mg / kg. Spray mushroom water and keep it for 4-5 days.

[0015] 8) Cultivating mushrooms: After the mushroom buds are formed, the temperature in the mushroom house is controlled to drop to 13-15°C, and the material temperature is maintained at 15-16°C to cultivate the growth of fruiting bodies;

[0016] 9) When the fruiting bodies grow to 7-8 months of maturity and the mushroom body is 4.5-5.5 cm high, harvest them in time to obtain the giant stropharia.

[0017] Preferably, in step 1), the weight ratio of cotton residue is 18% to 22%, and the weight ratio of sawdust is 28% to 32%.

[0018] Preferably, in step 1), the weight ratio of each component in the mushroom culture medium is: 20% by weight of cotton residue, 30% by weight of sawdust, 29% by weight of rice husk, 20% by weight of corn cob, and 1% by weight of lime.

[0019] Preferably, in step 2), water is first added to the fruiting culture medium to a moisture content of 70% to 72%, and the mixture is piled for fermentation. After 6 days, the pile is raked open, fluffed, and water is added to a moisture content of 68% to 70%, and the mixture is piled for fermentation again. After 4 days, the pile is turned over, and water is added to a moisture content of 68% to 70%, and the mixture is piled for fermentation again. After 2 days, the pile is raked open for standby use.

[0020] Preferably, in step 3), after the culture medium in step 2) is moved into the greenhouse, it is evenly distributed to the bed frame and heated. After the material temperature reaches 45-48°C, it is maintained for 6 hours; after the temperature of the material layer is stable and consistent, the material temperature is gradually increased to 58-60°C at a rate of 1°C per hour and maintained for 8 hours; after pasteurization, the material temperature is reduced to 46-50°C within 10-12 hours, the air volume is adjusted by the frequency converter for internal circulation, and the ventilation valve is adjusted to maintain the culture medium temperature at 48-52°C for 2-3 days.

[0021] Preferably, in step 5), the insect-proof net is pulled down in the mushroom house and the insect-killing lamp is turned on.

[0022] Preferably, in step 8), after the mushroom buds are formed, the air temperature is controlled to drop to 13° C., and the material temperature is maintained at 15° C. to cultivate the growth of the fruiting bodies.

[0023] Preferably, in step 9), clean gloves should be worn when picking, and the mushrooms should be slightly pressed down first, and then gently rotated to avoid moving the surrounding small mushrooms; the picked large stropharia are placed in a plastic turnover box with air holes.

[0024] As a preferred embodiment, the method further comprises step 10) of tide change management. In step 10), the bed surface after the first tide of mushrooms has grown is leveled to keep the environment clean. The management of the second and third tides of mushrooms is the same as the management of the first tide of mushrooms until one crop of mushrooms is completed.

[0025] The beneficial effects of the present invention are:

[0026] 1. The present invention adopts a greenhouse layer-frame cultivation of Stropharia officinalis, and is equipped with facilities and equipment such as insect-proof nets and insect-killing lamps, which can effectively prevent crop pests from flying into the mushroom house and prevent fruiting bodies from being infested by insects. The proportion of fruiting bodies infested by insects is reduced from 9.8% in field cultivation to 3.2%. The insect-proof operation is simple and quick, which effectively reduces the problem of insect pests. The insect-proof operation is practical and easy to promote.

[0027] 2. The present invention adopts cotton residue and sawdust as the culture medium for mushroom production. After the mushroom buds are formed, the temperature of the mushroom house is controlled at about 13°C and the material temperature is controlled at about 16°C, which greatly improves the quality of the fruiting body, thereby increasing the high-quality mushroom rate to 65%-71%, which is twice that of the field cultivation with ordinary culture medium. At the same time, cotton residue and sawdust are effectively utilized, with significant economic and ecological benefits.

[0028] 3. The present invention makes full use of infrastructure, can quickly obtain a large number of high-quality Stropharia officinalis fruiting bodies, is suitable for factory production, and can produce 3 to 4 crops of Stropharia officinalis each year. At the same time, the method is also suitable for cultivating Stropharia officinalis under natural conditions. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 The fruiting body of Stropharia rugosa obtained by the cultivation method in Example 2;

[0030] Figure 2 The invention is a real object of the Stropharia officinalis fruiting body obtained by adopting the cultivation method in the comparative example. DETAILED DESCRIPTION

[0031] The present invention is further described in detail by the following examples, but the content of the present invention is not limited thereto.

[0032] Embodiment 1:

[0033] 1) Preparation of mushroom culture medium: Under natural climatic conditions, the mushroom culture medium is prepared in September to October. Mix 15% cotton residue, 35% sawdust, 30% rice husk, 19% corn cob and 1% lime and set aside;

[0034] 2) Composting and fermenting: adding water to the fruiting culture material in step 1) to a water content of 70% to 72%, composting and fermenting, raking and fluffing the compost after 6 days, and replenishing water to a water content of 68% to 70%, and composting and fermenting again; turning the compost after 4 days, and replenishing water to a water content of 68% to 70%, and composting and fermenting again; raking and opening the compost after 2 days for standby use;

[0035] 3) Secondary fermentation in greenhouse: move the culture medium from step 2) into the greenhouse, evenly distribute the culture medium to the bed frame and heat it, and maintain it for 6 hours after the temperature reaches 45-48℃; after the temperature of the material layer is stable and consistent, gradually increase the temperature to 58-60℃ at a rate of 1℃ per hour and maintain it for 8 hours; after pasteurization, reduce the temperature to 46-50℃ within 10-12 hours, adjust the air volume through the frequency converter, perform internal circulation, and adjust the ventilation valve to keep the culture medium temperature at 48-52℃ for 2-3 days. The secondary fermentation takes about 5-6 days, and it is the fermentation culture medium of Stropharia rugosa after it is finished;

[0036] 4) Sowing: Inoculate the Stropharia occidentalis cultivar into the culture medium prepared in step 3) by hole sowing, with a seeding rate of 0.5-0.7 kg / m2 ;

[0037] 5) Sterilization: Cover the Stropharia officinalis fermentation culture medium in step 4) with a film, pull down the insect-proof net in the greenhouse, turn on the insect-killing lamp, maintain the air temperature at 20-25°C, the material temperature at 24-26°C, the relative humidity in the mushroom house at 78-82%, and the CO2 at 2500-3500 mg / kg. Sterilization time is 30-35 days;

[0038] 6) Covering with soil: Covering the fermented culture medium of Stropharia officinalis in step 5) with soil to a thickness of 3 to 3.5 cm, maintaining the air temperature at 20 to 25° C., and the material temperature at 24 to 26° C. for 10 to 13 days;

[0039] 7) Bud formation: Control the material temperature to drop to 19℃ at an average rate of 1℃ per day, and the air temperature to around 17℃. Increase fresh air to evenly reduce the CO2 concentration in the mushroom house every day, and eventually drop it to 1000~1500mg / kg. Spray mushroom water and keep it for 4~5 days.

[0040] 8) Cultivating mushrooms: After the mushroom buds are formed, the temperature is controlled to drop to 13-15°C, and the material temperature is maintained at 15-17°C to cultivate the growth of fruiting bodies;

[0041] 9) When the fruiting bodies grow to 7-8 years of maturity and the mushroom bodies are 4.5-5.5 cm high, harvest them in time to get the big stropharia. Wear clean gloves when picking, press down slightly first, then gently rotate to pick them, and avoid moving the surrounding small mushrooms. The harvested fruiting bodies should be placed in a plastic turnover box with air holes;

[0042] 10) Management during the transition period: After the mushrooms have grown, the bed surface should be leveled and the environment should be kept clean. The management of the second and third waves of mushrooms should be the same as the management of the first wave until the end.

[0043] Embodiment 2:

[0044] 1) Preparation of mushroom culture medium: Factory-based production can prepare mushroom culture medium according to annual needs. Mix 20% cotton residue, 30% sawdust, 29% rice husk, 20% corn cob and 1% lime and set aside;

[0045] 2) Composting and fermenting: adding water to the fruiting culture material in step 1) to a water content of 70% to 72%, composting and fermenting, raking and fluffing the compost after 6 days, and replenishing water to a water content of 68% to 70%, and composting and fermenting again; turning the compost after 4 days, and replenishing water to a water content of 68% to 70%, and composting and fermenting again; raking and opening the compost after 2 days for standby use;

[0046] 3) Secondary fermentation in greenhouse: move the culture medium from step 2) into the greenhouse, evenly distribute the culture medium to the bed frame and heat it, and maintain it for 6 hours after the temperature reaches 45-48℃; after the temperature of the material layer is stable and consistent, gradually increase the temperature to 58-60℃ at a rate of 1℃ per hour and maintain it for 8 hours; after pasteurization, reduce the temperature to 46-50℃ within 10-12 hours, adjust the air volume through the frequency converter, perform internal circulation, and adjust the ventilation valve to keep the culture medium temperature at 48-52℃ for 2-3 days. The secondary fermentation takes about 5-6 days, and it is the fermentation culture medium of Stropharia rugosa after it is finished;

[0047] 4) Sowing: Inoculate the Stropharia occidentalis cultivar into the culture medium prepared in step 3) by hole sowing, with a seeding rate of 0.5-0.7 kg / m 2 ;

[0048] 5) Sterilization: Cover the Stropharia officinalis fermentation culture medium in step 4) with a film, pull down the insect-proof net in the greenhouse, turn on the insect-killing lamp, maintain the air temperature at 20-25°C, the material temperature at 24-26°C, the relative humidity in the mushroom house at 78-82%, and the CO2 at 2500-3500 mg / kg. Sterilization time is 30-35 days;

[0049] 6) Covering with soil: Covering the fermented culture medium of Stropharia officinalis in step 5) with soil to a thickness of 3 to 3.5 cm, maintaining the air temperature at 20 to 25° C., and the material temperature at 24 to 26° C. for 10 to 13 days;

[0050] 7) Bud formation: Control the material temperature to drop to 19℃ at an average rate of 1℃ per day, and the air temperature to around 17℃. Increase fresh air to evenly reduce the CO2 concentration in the mushroom house every day, and eventually drop it to 1000~1500mg / kg. Spray mushroom water and keep it for 4~5 days.

[0051] 8) Cultivating mushrooms: After the mushroom buds are formed, the temperature is controlled to drop to 13-15°C, and the material temperature is maintained at 15-17°C to cultivate the growth of fruiting bodies;

[0052] 9) When the fruiting bodies grow to 7-8 years of maturity and the mushroom bodies are 4.5-5.5 cm high, harvest them in time to get the big stropharia. Wear clean gloves when picking, press down slightly first, then gently rotate to pick them, and avoid moving the surrounding small mushrooms. The harvested fruiting bodies should be placed in a plastic turnover box with air holes;

[0053] 10) Management during the transition period: After the mushrooms have grown, the bed surface should be leveled and the environment should be kept clean. The management of the second and third waves of mushrooms should be the same as the management of the first wave until the end.

[0054] Embodiment 3:

[0055] This example is a comparative test between Example 2 and a conventional cultivation method (hereinafter referred to as "Comparative Example") to detect the effects of different cultivation methods on Stropharia rugosa.

[0056] Comparative example: adopting the field cultivation method, the cultivation season is October, and the rice straw is processed and dried for later use. The formula ratio of the mushroom culture medium is: rice straw 99% + lime 1%; the natural length of the rice straw is used as the width, and the heads are stacked crosswise along the ridge direction when discharged, with a height of about 35cm and a spacing of 60cm between each ridge. After the ridge bed is discharged, water can be diverted to irrigate the field. After the rice straw is soaked for 2 days, turn the upper and lower rice straws, continue to soak for 2 days, and then drain the excess field water. Rice straw dosage: dry rice straw 25kg / m 2 . Use the spot sowing method to break the fungus into 2-3 cm blocks, and sow them on the straw in rows, with a sowing width of 10-12 cm. Use the straw on the ridge to cover the fungus in the front row, with a covering thickness of 2-3 cm. Soil is covered while sowing. Dig up the soil in the furrow and break it into small pieces to cover the straw, with a covering thickness of 3 cm. The mycelium begins to germinate 2-3 days after sowing. The mycelium begins to eat the material 3-4 days later. After 20 days, the mycelium has occupied more than 1 / 2 of the culture medium, and water should be sprayed lightly; when the mycelium has eaten more than 2 / 3 of the material, cover the exposed straw head with soil in combination with ditch cleaning. Natural fruiting, when the mushroom buds grow to a diameter of 2 cm, the amount of water spraying can be appropriately reduced and harvested in time.

[0057] Test location: The test was conducted in the edible fungus test room of Zhejiang Academy of Agricultural Sciences;

[0058] Test period: 2021-2022;

[0059] Experimental design: 50 m3 of each of the mushroom culture medium of Example 2 and the comparative example was used. 2 , respectively, cultivated Stropharia cap mushrooms, and counted the number of insect pests and high-quality mushrooms of Stropharia cap mushrooms obtained by the two cultivation methods during the harvest period, calculated the insect pest rate and high-quality mushroom rate, measured the cap diameter, cap thickness, stipe diameter, stipe length and single mushroom weight of Stropharia cap mushrooms and calculated the average value, and calculated the yield of Stropharia cap mushroom weight per mu; repeated 3 times, calculated the average value, and the results are shown in Tables 1 and 2 below.

[0060] Among them, the pest rate = the number of pest-infested Stropharia mushrooms / the total number of harvested Stropharia mushrooms*100%, the high-quality mushroom rate = the number of high-quality Stropharia mushrooms / the total number of harvested Stropharia mushrooms*100%; according to the requirements of market statistics for first-class mushrooms, the standards for high-quality Stropharia mushrooms are: the cap is light red to ball red, the fruiting body is not open, the inner veil is not broken, the stem is straight, not hollow, white, the cap thickness ≥20mm, the cap diameter ≥35mm, the stem length ≤60mm, the stem diameter ≥35mm, and the single mushroom weight ≥30g.

[0061] Table 1: Comparison of the insect pest rate and high-quality mushroom rate of Stropharia officinalis fruiting bodies under different cultivation methods

[0062] Group Pest control rate (%) High-quality mushroom rate (%) Example 2 3.2％ 68.7％ Comparative Example 9.8％ 31.1％

[0063] Table 2: Comparison of the characteristics of Stropharia officinalis fruiting bodies cultivated by different methods

[0064]

[0065] Result analysis: The fruiting bodies of Pleurotus eryngii obtained by the cultivation method in Example 2 are as follows: Figure 1 As shown, the fruiting bodies of Pleurotus eryngii obtained by the cultivation method in the comparative example are as follows Figure 2 As shown, after statistical measurement, the high-quality mushroom rate of the Stropharia officinalis fruiting body obtained by the cultivation method in Example 2 is 68.7%, which is twice that of the field cultivation method using ordinary culture medium, and the insect pest rate is 3.2%, which is 2 / 3 lower than the insect pest rate of the field cultivation method using ordinary culture medium.

[0066] In the market, the purchase price of first-class mushrooms is 1.5-2.5 times that of second-class mushrooms and 2-3 times that of third-class mushrooms. After adopting the stropharia cultivation method of Example 2, the average cap thickness, average cap diameter, average stipe length, average stipe diameter and average single mushroom weight of stropharia fruiting bodies meet the requirements of first-class mushrooms in the market, which greatly improves the quality of stropharia fruiting bodies. The economic benefits of adopting this stropharia cultivation method are good.

Claims

1. A cultivation method for improving the quality of Stropharia officinalis fruiting bodies, characterized in that: The following steps are involved: 1) Preparation of mushroom culture medium: Mix 20% cotton residue, 30% sawdust, 29% rice husk, 20% corn cob and 1% lime and set aside; 2) Composting and fermenting: first add water to the fruiting culture medium to a moisture content of 70% to 72%, and then compost and ferment. After 6 days, rake and loosen the compost, and add water to a moisture content of 68% to 70%, and then compost and ferment again. After 4 days, turn the compost, and add water to a moisture content of 68% to 70%, and then compost and ferment again. After 2 days, rake the compost and set aside. 3) Secondary fermentation in greenhouse: After the culture medium in step 2) is moved into the greenhouse, it is evenly distributed to the bed frame and heated. After the material temperature reaches 45-48°C, it is maintained for 6 hours; after the temperature of the material layer is stable and consistent, the material temperature is gradually increased to 58-60°C at a rate of 1°C per hour and maintained for 8 hours; after the pasteurization is completed, the material temperature is reduced to 46-50°C within 10-12 hours, the air volume is adjusted by the frequency converter, internal circulation is carried out, and the ventilation valve is adjusted to keep the culture medium temperature at 48-52°C for 2-3 days; after the secondary fermentation is completed, it is the fermentation culture medium of Stropharia rugosa; 4) Sowing: Inoculate the Stropharia occidentalis cultivar into the culture medium prepared in step 3) by hole sowing, with a seeding rate of 0.5-0.7 kg / m 2 ; 5) Incubation: Cover the fermentation medium of Stropharia officinalis in step 4) with a film, pull down the insect-proof net in the mushroom house, turn on the insect-killing lamp, maintain the temperature in the mushroom house at 20-25℃, the medium temperature at 24-26℃, the relative humidity in the mushroom house at 78-82%, and the CO2 concentration at 2500-3500mg / kg. The incubation time is 30-35d. 6) Covering with soil: Cover the fermentation culture medium of Stropharia officinalis in step 5) with soil with a thickness of 3 to 3.5 cm, maintain the temperature in the mushroom house at 20 to 25°C, and the culture medium temperature at 24 to 26°C for 10 to 13 days; 7) Inducing bud formation: Control the material temperature to drop to 19℃ at an average rate of 1℃ per day, and the temperature in the mushroom house to about 17℃. Increase fresh air to evenly reduce the CO2 concentration in the mushroom house every day, and finally drop it to 1000-1500mg / kg. Spray mushroom water and keep it for 4-5 days. 8) Cultivating mushrooms: After the mushroom buds are formed, the temperature in the mushroom house is controlled to drop to 13-15℃, and the material temperature is maintained at 15-16℃ to cultivate the growth of fruiting bodies; 9) When the fruiting body grows to 7-8 maturity and the mushroom body is 4.5-5.5cm high, harvest it in time to get the giant stropharia.

2. A cultivation method for improving the quality of Stropharia officinalis fruiting bodies according to claim 1, characterized in that: In step 8), after the mushroom buds are formed, the air temperature is controlled to drop to 13°C and the material temperature is maintained at 15°C to cultivate the growth of the fruiting bodies.

3. A cultivation method for improving the quality of Stropharia officinalis fruiting bodies according to claim 1, characterized in that: In step 9), wear clean gloves when picking, press down slightly first, then gently rotate to pick, avoid moving the surrounding small mushrooms; put the picked large stropharia into a plastic turnover box with air holes.

4. A cultivation method for improving the quality of Stropharia officinalis fruiting bodies according to claim 1, characterized in that: The method also includes step 10) of tide change management. In step 10), the bed surface after the first tide of mushrooms is smooth and the environment is kept clean. The management of the second and third tides of mushrooms is the same as the management of the first tide of mushrooms until one crop of mushrooms is completed.

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