Microfluidic chip and microfluidic chip detection system

Abstract

The present invention relates to a microfluidic chip and a microfluidic chip detection system. The microfluidic chip includes: a storage member having a groove thereon, and at least two storage bins are arranged around the groove; a base, which is arranged at one end of the storage member away from the groove, and a reaction bin is arranged on the base; and a valve, which is arranged in the groove, and the valve is configured to operably connect any one of the at least two storage bins to the reaction bin. The present invention can guide the solution in any storage bin to the reaction bin, or guide the solution in the reaction bin to any storage bin by operating the valve, so as to realize solution transfer. The structure is simple and compact, and the flow channel length can be greatly shortened, thereby improving the detection efficiency.

Description

Technical Field

[0001] The present invention relates to the field of in vitro diagnosis, and in particular to a microfluidic chip and a microfluidic chip detection system. Background Art

[0002] Nucleic acid detection technology is a technology that directly detects the genetic material of living organisms, such as DNA and RNA. It has extremely high specificity and sensitivity, a short window period, and multiple detection capabilities. However, the nucleic acid detection process is very complicated and has many steps. It has high requirements for the detection environment, laboratory conditions, and personnel technical level. Therefore, the development trend of nucleic acid detection is fully automatic integration, high integration, bedside detection, instant detection, and detection anywhere.

[0003] In order to achieve the above-mentioned fully automatic integrated nucleic acid detection, the microfluidics technology that has emerged in recent years integrates the cumbersome nucleic acid detection process into a chip with micro-sized flow channels and cavities arranged in a certain pattern. Different biological reagents are released in a certain order and flow through different flow channels to the designated cavity to complete various biochemical reactions, and finally achieve rapid and accurate detection of nucleic acids. Thanks to this form of implementation, nucleic acid detection based on microfluidics technology has the advantages of being fully automated, highly integrated, simple and fast, avoiding cross contamination, being able to be used independently in a variety of environments, and not requiring highly professional personnel, which meets the concept and requirements of rapid detection. Summary of the invention

[0004] Some embodiments of the present invention provide a microfluidic chip and a microfluidic chip detection system suitable for rapid in vitro detection.

[0005] In one aspect of the present invention, a microfluidic chip is provided, comprising:

[0006] A storage member having a groove thereon and at least two storage bins arranged around the groove;

[0007] A base, disposed at an end of the storage element away from the groove, and a reaction chamber is disposed on the base; and

[0008] A valve is disposed in the groove, and the valve is configured to operably connect any one of at least two storage chambers to the reaction chamber.

[0009] In some embodiments, at least two first storage component internal flow channels are provided in the storage component, each first storage component internal flow channel corresponds to a storage bin, and a valve internal flow channel connected to the reaction bin is provided in the valve, and the valve is configured to operably connect the valve internal flow channel with any first storage component internal flow channel.

[0010] In some embodiments, the first end of the flow channel in each first storage member passes through the bottom wall of the groove, the valve is configured to operably connect the flow channel in the valve with the first end of the flow channel in the first storage member, and the second end of the flow channel in each first storage member is connected to the storage bin through a side of the storage bin adjacent to the base.

[0011] In some embodiments, the second end of the flow channel in the first storage member is connected to the lowest part of the storage bin.

[0012] In some embodiments, the first end and the second end of the flow channel in the valve both pass through an end of the bottom wall of the valve adjacent to the groove, the first end of the flow channel in the valve is connected to the reaction chamber, the second end of the flow channel in the valve is operably connected to the flow channel in any first storage element, the first end of the flow channel in the valve is located in the middle of the valve, and the second end of the flow channel in the valve is close to the outer edge of the valve.

[0013] In some embodiments, the valve comprises:

[0014] a rotor rotatably disposed in the groove, the rotor comprising a valve seat and a valve stem, the valve stem being connected to the valve seat; and

[0015] The valve cover is connected to the circumferential side wall of the groove and abuts against the valve seat to limit the valve seat between the valve cover and the bottom wall of the groove. The valve cover is provided with a first through hole, and the operating part of the valve stem passes through the first through hole. The operating part of the valve stem is configured to be connected to an external operating part.

[0016] In some embodiments, the valve cover abuts against a circumferential edge of the valve seat.

[0017] In some embodiments, the microfluidic chip also includes a sealing film, the at least two storage chambers include a reagent chamber, the sealing film is configured to seal the reagent chamber, the microfluidic chip also includes a top cover and a puncture needle, the top cover is provided at one end of the storage element where the groove is provided, the puncture needle is connected to the top cover, and the puncture needle is configured to be pressed toward the sealing film under the action of an external force to puncture the sealing film.

[0018] In some embodiments, the top cover includes a first rib, the puncture needle is connected to the first rib, and the first rib is configured to be broken under the action of an external force so that the puncture needle is separated from the top cover and pressed toward the sealing film.

[0019] In some embodiments, a second through hole is provided in the middle of the top cover, and the second through hole is configured to allow an external operating member to pass through to operate the valve.

[0020] In some embodiments, an intra-needle airway is provided in the puncture needle, and a third through hole connecting the outside of the puncture needle and the intra-needle airway is provided near the portion where the puncture needle is connected to the top cover.

[0021] In some embodiments, the microfluidic chip also includes a cover sheet, which is disposed inside the top cover, and the cover sheet is provided with a fourth through hole allowing the puncture needle to pass through. The puncture needle is configured to be pressed toward the sealing film under the action of an external force, and continues to be pressed toward the sealing film after puncturing the sealing film, so that the third through hole is sealed by the cover sheet.

[0022] In some embodiments, the reaction chamber protrudes toward a side away from the storage element.

[0023] In some embodiments, the reaction chamber is a spherical crown structure.

[0024] In some embodiments, the microfluidic chip also includes an amplification component, which is provided with an amplification chamber, and a slot is provided on the side of the storage component, and the slot is located between the two adjacent storage chambers. The amplification component is plugged into the slot, and the valve is configured to operably connect the reaction chamber with the amplification chamber.

[0025] In some embodiments, the storage component is provided with a second storage component internal flow channel, a first end of the second storage component internal flow channel passes through the slot, a second end of the second storage component internal flow channel passes through the groove, the amplification component is provided with an amplification component internal flow channel connected to the amplification chamber, the amplification component internal flow channel is connected to the first end of the second storage component internal flow channel, and the valve is configured to operably connect the second end of the second storage component internal flow channel to guide the solution in the reaction chamber to the amplification chamber through the second storage component internal flow channel and the amplification component internal flow channel.

[0026] In some embodiments, the storage component is provided with a third storage component internal flow channel, the first end of the third storage component internal flow channel passes through the slot, the second end of the third storage component internal flow channel passes through the groove, the amplification component is provided with an amplification component internal air channel connected to the amplification chamber, the amplification component internal air channel is connected to the first end of the third storage component internal flow channel, and the valve is configured to operably connect the second end of the third storage component internal flow channel to guide the gas in the amplification chamber to a storage chamber through the amplification component internal air channel and the third storage component internal flow channel.

[0027] In some embodiments, the valve is provided with an intra-valve flow channel connected to the reaction chamber, and the valve is also provided with an intra-valve air channel. The valve is configured to operably connect the intra-valve flow channel to the reaction chamber and the amplification chamber, and to connect the intra-valve air channel to the amplification chamber and a storage chamber.

[0028] In some embodiments, the storage component is provided with an internal air passage in the storage component, the internal air passage in the storage component is connected to the reaction chamber, and the internal air passage in the storage component is configured to be connected to an external air pump.

[0029] In some embodiments, the first end of the air channel in the storage element passes through one end of the groove provided in the storage element, and the first end of the air channel in the storage element is located between two adjacent storage bins.

[0030] In some embodiments, the top cover is fixedly disposed on an end of the storage component where the groove is disposed, and the base is fixedly disposed on an end of the storage component away from the top cover.

[0031] In one aspect of the present invention, a microfluidic chip detection system is provided, comprising a detection device and the above-mentioned microfluidic chip, wherein the detection device comprises an operating table for accommodating the microfluidic chip and an operating member for operating the valve.

[0032] Based on the above technical solution, the present invention has at least the following beneficial effects:

[0033] In some embodiments, a groove is provided on the storage member, at least two storage bins are provided around the circumference of the groove, a valve is provided in the groove, and a reaction bin is provided below the valve. By operating the valve, the solution in any storage bin can be directed to the reaction bin, or the solution in the reaction bin can be directed to any storage bin, thereby realizing solution transfer. The structure is simple and compact, can greatly shorten the flow channel length, and improve detection efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] The drawings described herein are used to provide a further understanding of the present invention and constitute a part of this application. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation of the present invention. In the drawings:

[0035] Figure 1 A schematic diagram of the overall structure of a microfluidic chip provided according to some embodiments of the present invention;

[0036] Figure 2 A schematic diagram of the exploded structure of a microfluidic chip provided according to some embodiments of the present invention;

[0037] Figure 3 A schematic diagram of the structure of a top cover of a microfluidic chip provided according to some embodiments of the present invention;

[0038] Figure 4 A schematic diagram of the structure of a top cover and a cover sheet of a microfluidic chip provided according to some embodiments of the present invention;

[0039] Figure 5 A schematic diagram of the structure of a storage element and a valve of a microfluidic chip provided according to some embodiments of the present invention;

[0040] Figure 6 A schematic top view of a storage element of a microfluidic chip according to some embodiments of the present invention;

[0041] Figure 7 A bottom view schematically showing a storage element of a microfluidic chip according to some embodiments of the present invention;

[0042] Figure 8 A schematic cross-sectional view of a storage element of a microfluidic chip according to some embodiments of the present invention;

[0043] Fig. 9 A schematic diagram of the exploded structure of a valve of a microfluidic chip provided according to some embodiments of the present invention;

[0044] Fig.10 A schematic cross-sectional view of a valve of a microfluidic chip according to some embodiments of the present invention;

[0045] Fig.11 A schematic diagram of the structure of a base of a microfluidic chip provided according to some embodiments of the present invention;

[0046] Fig.12 A schematic top view of a base of a microfluidic chip provided according to some embodiments of the present invention;

[0047] Fig.13 A schematic diagram of the bottom structure of a base of a microfluidic chip provided according to some embodiments of the present invention;

[0048] Fig.14 A schematic cross-sectional view of a base of a microfluidic chip provided according to some embodiments of the present invention;

[0049] Fig.15 A schematic diagram of an amplification component of a microfluidic chip provided according to some embodiments of the present invention;

[0050] Fig.16 A schematic diagram of an amplification component, a second gasket, and a storage component of a microfluidic chip provided according to some embodiments of the present invention before being connected;

[0051] Fig.17 This is a schematic diagram of the microfluidic chip according to some embodiments of the present invention before the amplification component is inserted into the slot of the storage component.

[0052] The reference numerals in the accompanying drawings are described as follows:

[0053] 1-storage element; 11-groove; 12-storage compartment; 121-reagent compartment; 13-flow channel in the first storage element; 131-first end of the flow channel in the first storage element; 132-second end of the flow channel in the first storage element; 14-slot; 141-first buckle; 15-flow channel in the second storage element; 151-first end of the flow channel in the second storage element; 152-second end of the flow channel in the second storage element; 16-flow channel in the third storage element; 161-first end of the flow channel in the third storage element; 162-second end of the flow channel in the third storage element; 17-air channel in the storage element; 171-first end of the air channel in the storage element; 18-flow channel in the fourth storage element; 181-first end of the flow channel in the fourth storage element;

[0054] 2-base; 21-reaction chamber; 22-chassis; 23-support member; 24-positioning member; 25-clamping groove; 261-first end of the flow channel in the chassis; 262-second end of the flow channel in the chassis; 27-positioning protrusion; 28-positioning groove;

[0055] 3-valve; 31-flow channel in the valve; 311-first end of the flow channel in the valve; 312-second end of the flow channel in the valve; 32-rotor; 321-valve seat; 3211-valve seat body; 3212-first gasket; 322-valve stem; 324-boss; 33-valve cover; 331-first through hole; 34-air channel in the valve;

[0056] 4-top cover; 41-puncture needle; 411-needle inner airway; 412-third through hole; 421-first rib; 422-second rib; 43-second through hole; 44-fifth through hole; 45-ring member; 46-sample injection port; 47-cover plate; 48-circumferential side wall; 49-cylindrical member;

[0057] 5-amplification component; 51-amplification chamber; 52-inner flow channel of the amplification component; 53-inner air channel of the amplification component; 54-second buckle;

[0058] 6-cover plate; 61-fourth through hole; 62-sixth through hole; 63-seventh through hole;

[0059] 7- Second gasket.

[0060] It should be understood that the size of each part shown in the accompanying drawings is not drawn according to the actual proportional relationship. In addition, the same or similar reference numerals represent the same or similar components. DETAILED DESCRIPTION

[0061] Various exemplary embodiments of the present invention will now be described in detail with reference to the accompanying drawings. The description of the exemplary embodiments is merely illustrative and is in no way intended to limit the present invention and its application or use. The present invention can be implemented in many different forms and is not limited to the embodiments described herein. These embodiments are provided to make the present invention thorough and complete and to fully express the scope of the present invention to those skilled in the art. It should be noted that unless otherwise specifically stated, the relative arrangement of the parts and steps, the composition of the materials, the numerical expressions and the numerical values ​​set forth in these embodiments should be interpreted as being merely exemplary, rather than as limitations.

[0062] The words "first", "second" and similar words used in the present invention do not indicate any order, quantity or importance, but are only used to distinguish different parts. The words "include" or "comprise" and similar words mean that the elements before the word include the elements listed after the word, and do not exclude the possibility of including other elements. "Up", "down", "left", "right" and the like are only used to indicate relative positional relationships. When the absolute position of the described object changes, the relative positional relationship may also change accordingly.

[0063] In the present invention, when a specific device is described as being located between a first device and a second device, there may or may not be an intermediate device between the specific device and the first device or the second device. When a specific device is described as being connected to other devices, the specific device may be directly connected to the other devices without an intermediate device, or may not be directly connected to the other devices but have an intermediate device.

[0064] All terms (including technical terms or scientific terms) used in the present invention have the same meanings as those understood by ordinary technicians in the field to which the present invention belongs, unless otherwise specifically defined. It should also be understood that terms defined in general dictionaries should be interpreted as having meanings consistent with their meanings in the context of the relevant technology, and should not be interpreted in an idealized or extremely formal sense, unless explicitly defined herein.

[0065] Technologies, methods, and equipment known to ordinary technicians in the relevant art may not be discussed in detail, but where appropriate, the technologies, methods, and equipment should be considered part of the specification.

[0066] like Figure 1 and Figure 2 As shown, some embodiments provide a microfluidic chip, which includes a storage element 1, a base 2 and a valve 3.

[0067] like Figure 5 and Figure 6 As shown, a groove 11 is provided on the storage member 1, and at least two storage bins 12 are provided around the groove 11. The groove 11 includes a bottom wall and a circumferential side wall.

[0068] like Figure 1 and Figure 2 As shown, the base 2 is disposed at one end of the storage element 1 away from the groove 11 , and a reaction chamber 21 is disposed on the base 2 .

[0069] like Figure 1 , Figure 2 and Figure 5 As shown, the valve 3 is disposed in the groove 11 , and the valve 3 is configured to operably connect any one of the at least two storage chambers 12 with the reaction chamber 21 .

[0070] The at least two storage bins 12 include at least one sample bin and at least two reagent bins 121. The reagent bins 121 are used to store different detection reagents, which may be solid or liquid reagents. The number of reagent bins 121 can be flexibly increased or decreased according to demand. One sample bin can be provided, or two can be provided as needed. The sample bins are used to add samples to be tested, and the samples to be tested include blood or saliva, etc.

[0071] The reaction chamber 21 is a reagent reaction site for nucleic acid extraction.

[0072] Valve 3 is a key component for fluid flow control in the microfluidic chip, controlling the connection and closure of the flow channel.

[0073] The base 2 is used to ensure that the microfluidic chip is placed stably and flatly, and also has positioning and limiting functions to improve detection stability.

[0074] In the embodiment of the present disclosure, a groove 11 is provided on the storage element 1, at least two storage bins 12 are provided around the circumference of the groove 11, a valve 3 is provided in the groove 11, and a reaction bin 21 is provided below the valve 3. By operating the valve 3, the solution in any storage bin 12 can be directed to the reaction bin 21, or the solution in the reaction bin 21 can be directed to any storage bin 12, thereby realizing solution transfer. The structure is simple and compact, and the flow path length can be greatly shortened, thereby improving the detection efficiency.

[0075] like Figure 8 As shown, at least two first storage member internal flow channels 13 are provided in the storage member 1, and each first storage member internal flow channel 13 is connected to a storage bin 12. Fig.10 As shown, the valve 3 is provided with an inner valve flow channel 31 communicating with the reaction chamber 21 , and the valve 3 is configured to operably connect the inner valve flow channel 31 with any inner flow channel 13 of the first storage element.

[0076] The flow channel 31 in the valve is always connected to the reaction chamber 21. By operating the valve 3, the flow channel 31 in the valve can be selectively connected to any one of the flow channels 13 in the first storage element to guide the solution in the storage chamber 12 to the reaction chamber 21, or to guide the solution in the reaction chamber 21 to the storage chamber 12.

[0077] In some embodiments, Figures 6 to 8 As shown, the first end 131 of the flow channel 13 in each first storage element passes through the bottom wall of the groove 11, and the valve 3 is configured to operably connect the flow channel 31 in the valve with the first end 131 of the flow channel 13 in the first storage element, and the second end 132 of the flow channel 13 in each first storage element is connected to the storage bin 12 through a side of the storage bin 12 adjacent to the base 2.

[0078] In some embodiments, Figures 6 to 8 As shown, the second end 132 of the flow channel 13 in the first storage member is connected to the lowest part of the storage bin 12 and the part closest to the groove 11 .

[0079] In some embodiments, Figures 6 to 8 As shown, the second end 132 of the flow channel 13 in the first storage member is connected to the lowest part of the storage bin 12 and the part closest to the groove 11 to shorten the length of the flow channel.

[0080] In some embodiments, Fig. 9 and Fig.10 As shown, the first end 311 and the second end 312 of the valve internal flow channel 31 both pass through one end of the bottom wall of the valve 3 adjacent to the groove 11, the first end 311 of the valve internal flow channel 31 is connected to the reaction chamber 21, the second end 312 of the valve internal flow channel 31 is operably connected to any first storage element internal flow channel 13, the first end 311 of the valve internal flow channel 31 is located in the middle of the valve 3, and the second end 312 of the valve internal flow channel 31 is close to the outer edge of the valve 3.

[0081] In some embodiments, Figure 5 and Figure 8 As shown, the storage element 1 is cylindrical in shape as a whole.

[0082] In some embodiments, Figure 2 , Fig. 9 and Fig.10 As shown, the valve 3 includes a rotor 32 and a valve cover 33 .

[0083] The rotor 32 is rotatably disposed in the groove 11 . The rotor 32 includes a valve seat 321 and a valve stem 322 . The valve stem 322 is connected to the valve seat 321 .

[0084] The valve cover 33 is connected to the circumferential side wall of the groove 11 and abuts against the valve seat 321, limiting the valve seat 321 between the valve cover 33 and the bottom wall of the groove 11. The valve seat 321 abuts against the bottom wall of the groove 11. A first through hole 331 is provided on the valve cover 33. The operating part of the valve stem 322 passes through the first through hole 331, and the operating part of the valve stem 322 is configured to be connected to an external operating part.

[0085] The valve seat 321 includes a valve seat body 3211 and a first gasket 3212. The first gasket 3212 is consistent with the bottom shape of the valve seat body 3211. The valve seat body 3211 and the first gasket 3212 are fixedly arranged. The inner flow channel 31 is formed in the valve seat 321. The valve seat body 3211 is made of a hard material, and the first gasket 3212 is made of an elastic material. By adjusting the valve cover 33, a suitable abutting pressure is applied to the valve seat 321 of the rotor 21, so that the valve seat 321 abuts against the bottom wall of the groove 11, thereby making the inner flow channel 31 of the valve have the airtightness required for detection, and avoiding leakage at the connection between the inner flow channel 31 of the valve and the inner flow channel of the storage member.

[0086] In some embodiments, the valve cover 33 abuts against a circumferential edge of the valve seat 321 .

[0087] The first end 131 of each first storage element inner flow channel 13 passes through the bottom wall of the groove 11 and is arranged around the central axis of the groove 11. The valve cover 33 abuts against the circumferential edge of the valve seat 321, which enables the valve seat 321 to abut against the bottom wall of the groove 11, so that the connection between the valve inner flow channel 31 and the storage element inner flow channel is sealed to avoid leakage.

[0088] The radial dimension of the valve stem 322 is smaller than the radial dimension of the valve seat body 3211. One end of the valve stem 322 is fixedly connected to the valve seat body 3211, and the other end of the valve stem 322 is an operating part, which is used to pass through the first through hole 331 and connect with an external operating member. The valve stem 322 is rotated by the operating member, thereby driving the valve seat body 3211 and the first gasket 3212 to rotate, so as to selectively connect the second end 312 of the valve inner flow channel 31 with a first storage member inner flow channel 13.

[0089] Optionally, the operating portion of the valve stem 322 is configured as a hexagonal structure or a quadrangular structure.

[0090] A boss 324 is provided on the circumference of the valve seat body 3211 . The boss 324 has an arc-shaped outer contour to reduce friction between the circumference of the valve seat body 3211 and the circumferential side wall of the groove 11 when the valve seat body 3211 rotates relative to the groove 11 .

[0091] In some embodiments, Figures 3 to 5 As shown, the microfluidic chip also includes a sealing film, at least two storage chambers 12 include a reagent chamber 121, the sealing film is configured to seal the reagent chamber 121, the microfluidic chip also includes a top cover 4 and a puncture needle 41, the top cover 4 is provided at one end of the storage member 1 having a groove 11, the puncture needle 41 is connected to the top cover 4, and the puncture needle 41 is configured to be pressed toward the sealing film under the action of an external force to puncture the sealing film.

[0092] In some embodiments, Figures 3 to 5As shown, the top cover 4 includes a first rib 421, and the puncture needle 41 is connected to the first rib 421. The first rib 421 is configured to be disconnected under the action of an external force so that the puncture needle 41 is separated from the top cover 4 and pressed toward the sealing film.

[0093] In some embodiments, Figures 3 to 5 As shown, a second through hole 43 is provided in the middle of the top cover 4 , and the second through hole 43 is configured to allow an external operating member to pass through so as to connect to the operating part of the valve 3 for rotating the valve 3 .

[0094] In some embodiments, Figure 4 As shown, an inner needle airway 411 is provided in the puncture needle 41 , and a third through hole 412 is provided at the portion where the puncture needle 41 is connected to the top cover 4 , and the third through hole 412 communicates the outside of the puncture needle 41 with the inner needle airway 411 .

[0095] In some embodiments, Figure 4 As shown, the microfluidic chip further includes a cover sheet 6, which is disposed in the top cover 4. The cover sheet 6 is provided with a sixth through hole 62 that allows an external operating member to pass through to connect the rotor 32. The cover sheet 6 is also provided with a fourth through hole 61 that allows a puncture needle 41 to pass through. The puncture needle 41 is configured to press against the sealing film under the action of an external force, and continues to press against the sealing film after puncturing the sealing film, so that the third through hole 412 is sealed by the cover sheet 6.

[0096] like Figure 4 As shown, the puncture needle 41 includes a first needle segment and a second needle segment, the radial dimension of the second needle segment is larger than the radial dimension of the first needle segment. The second needle segment is connected to the first rib 421, the first needle segment is configured as a sharp needle for puncturing the sealing film, and the third through hole 412 is provided in the second needle segment.

[0097] The puncture needle 41 is pressed against the sealing film under the action of external force, and punctures the sealing film, so that the reagent chamber 121 is connected to the atmosphere through the needle inner airway 411 and the third through hole 412 on the puncture needle 41, which is convenient for subsequent extraction of reagents. At this time, the first needle section passes through the fourth through hole 61. After the test is completed, the puncture needle 41 is further pressed against the sealing film under the action of external force, and the third through hole 412 is sealed by the cover sheet 6, thereby sealing the reagent chamber 121 to prevent waste liquid from flowing out.

[0098] In some embodiments, a sealing film is disposed on the cover sheet 6 to seal the reagent chamber 121 .

[0099] In other embodiments, the sealing film is directly disposed on the reagent chamber 121 to seal the reagent chamber 121 .

[0100] In some embodiments, Figures 11 to 14 As shown, the reaction chamber 21 protrudes toward a side away from the storage element 1 .

[0101] In some embodiments, Figures 11 to 14 As shown, the reaction chamber 21 is in a spherical crown structure. The reaction chamber 21 is used as a reagent reaction site for nucleic acid extraction, and the extraction step is completed in a short time through the cooperation of the spherical crown chamber structure and the ultrasonic transducer.

[0102] In some embodiments, Figures 15 to 17 As shown, the microfluidic chip also includes an amplification component 5, and the amplification component 5 is provided with an amplification chamber 51. Figure 5 and Figure 6 As shown, a slot 14 is provided on the side of the storage component 1 , and the slot 14 is located between two adjacent storage chambers 12 . The amplification component 5 is plugged into the slot 14 , and the valve 3 is configured to operably connect the reaction chamber 21 with the amplification chamber 51 .

[0103] The amplification element 5 is a thin sheet structure with a large heating surface and is easy to collect sample fluorescence.

[0104] The amplification component 5 adopts a separate design and can be connected or separated from the main structure of the microfluidic chip (including the storage component 1) by plugging and unplugging, thereby improving versatility.

[0105] In some embodiments, Figures 5 to 8 As shown, the storage element 1 is provided with a second storage element inner flow channel 15, a first end 151 of the second storage element inner flow channel 15 passes through the slot 14, and a second end 152 of the second storage element inner flow channel 15 passes through the groove 11, as shown in FIG. Fig.15 As shown, the amplification component 5 is provided with an amplification component internal flow channel 52 connected to the amplification chamber 51, the amplification component internal flow channel 52 is connected to the first end 151 of the second storage component internal flow channel 15, and the valve 3 is configured to be operably connected to the second end 152 of the second storage component internal flow channel 15 to guide the solution in the reaction chamber 21 to the amplification chamber 51 through the second storage component internal flow channel 15 and the amplification component internal flow channel 52.

[0106] In some embodiments, Figures 5 to 8 As shown, the storage element 1 is provided with a third storage element inner flow channel 16, a first end 161 of the third storage element inner flow channel 16 passes through the slot 14, and a second end 162 of the third storage element inner flow channel 16 passes through the groove 11, as shown in FIG. Fig.15As shown, the amplification component 5 is provided with an amplification component internal air channel 53 connected to the amplification chamber 51, and the amplification component internal air channel 53 is connected to the first end 161 of the flow channel 16 in the third storage component. The valve 3 is configured to be operably connected to the second end 162 of the flow channel 16 in the third storage component, so as to guide the gas in the amplification chamber 51 to a storage chamber 12 through the amplification component internal air channel 53 and the third storage component internal flow channel 16, so as to maintain the air pressure balance in the amplification chamber 51. If there are a little more samples to be tested and the amplification chamber 51 cannot accommodate them, the excess liquid in the amplification chamber 51 can also be discharged to the storage chamber 12 through the above-mentioned connected air channel and flow channel to avoid leakage and contamination. The amplification component 5 includes a main body and a plug-in part, and the amplification chamber 51 is arranged in the main body, and the plug-in part is connected to the slot 14 in a plug-in manner.

[0107] The flow channel 52 and the air channel 53 in the amplification component are both connected to the amplification chamber 51, and parts of the flow channel 52 and the air channel 53 in the amplification component are located in the plug-in portion.

[0108] While the valve 3 is connected to the second end 152 of the second storage component inner flow channel 15 to guide the solution in the reaction chamber 21 to the amplification chamber 51 through the second storage component inner flow channel 15 and the amplification component inner flow channel 52, the valve 3 is also connected to the second end 162 of the third storage component inner flow channel 16 to guide the gas in the amplification chamber 51 to a storage chamber 12 through the amplification component inner gas channel 53 and the third storage component inner flow channel 16. Optionally, the storage chamber 12 is adjacent to the slot 14.

[0109] In some embodiments, Fig. 9 and Fig.10 As shown, the valve 3 is provided with an intra-valve flow channel 31 connected to the reaction chamber 21, and the valve 3 is also provided with an intra-valve air channel 34. The valve 3 is configured to operably connect the intra-valve flow channel 31 to connect the reaction chamber 21 and the amplification chamber 51, and to connect the intra-valve air channel 34 to connect the amplification chamber 51 and a storage chamber 12.

[0110] In some embodiments, Figures 5 to 7 As shown, the storage unit 1 is provided with an internal air passage 17, which is connected to the reaction chamber 21, and the internal air passage 17 is configured to be connected to an external air pump. The air pump provides a suction force to guide the solution in the storage chamber 12 into the reaction chamber 21 through the valve 3, or the air pump provides a blowing force to guide the solution in the reaction chamber 21 into the storage chamber 12 through the valve 3.

[0111] In some embodiments, Figures 5 to 7 As shown, the first end 171 of the air channel 17 in the storage element passes through one end of the storage element 1 where the groove 11 is provided, and the first end 171 of the air channel 17 in the storage element is located between two adjacent storage bins 12 .

[0112] In some embodiments, Figure 1As shown, the top cover 4 is fixedly arranged at one end of the storage member 1 where the groove 11 is arranged, and the base 2 is fixedly arranged at one end of the storage member 1 away from the top cover 4 .

[0113] In some embodiments, Figure 2 As shown, the storage element 1 is a cylindrical structure, the cover surface of the top cover 4 covering the storage element 1 is circular, the surface of the base 2 connected to the storage element 1 is circular, and the cover sheet 6 is circular. The groove 11 set in the storage element 1 is a cylindrical groove. The base 321 and the first gasket 3212 of the rotor 32 of the valve 3 are circular.

[0114] The following is combined with Figures 1 to 17 Some specific embodiments of the microfluidic chip are described in detail.

[0115] like Figure 1 and Figure 2 As shown, the microfluidic chip includes a storage component 1, a base 2, a valve 3, a top cover 4, an amplification component 5 and a cover sheet 6.

[0116] like Figure 1 and Figure 2 As shown, the top cover 4 is fixed on the top of the storage member 1, and part of the top of the storage member 1 is enclosed therein, and the cover sheet 6 is arranged between the top cover 4 and the top of the storage member 1. The base 2 is fixedly arranged at the bottom of the storage member 1. A groove 11 is provided at the middle position of the top of the storage member 1, which is concave toward the bottom. The valve 3 is arranged in the groove 11, and the operating part of the valve 3 extends out of the groove 11 and extends toward the top cover 4. The top cover 4 is provided with a through hole to allow the operating part of the valve 3 to pass through, or an external operating part extends into the through hole to connect with the operating part of the valve 3, so as to operate the valve 3. The expansion member 5 is pluggably arranged on the side of the storage member 1, and a notch is provided on the side of the top cover 4 to avoid the expansion member 5.

[0117] like Figures 2 to 4 As shown, the top cover 4 includes a circular cover plate 47, and a second through hole 43 is provided in the middle of the cover plate 47. The second through hole 43 is used to pass the operating part of the valve 3, or the external operating part extends into the second through hole 43 to connect with the operating part of the valve 3. The cover plate 47 is provided with a circumferential side wall 48 extending toward the storage member 1. The circumferential side wall 48 of the top cover 4 encloses the top part of the storage member 1. The circumferential side wall 48 of the top cover 4 is provided with a clamping block, and the top of the storage member 1 is provided with a clamping slot. The top cover 4 and the storage member 1 are fixedly connected through the clamping block and the clamping slot structure.

[0118] A sample loading port 46 is provided on the cover plate 47 of the top cover 4 . The sample loading port 46 corresponds to the position of one storage bin 12 among the plurality of storage bins 12 . The storage bin 12 serves as a sample loading bin, and the sample to be tested is added into the sample loading bin through the sample loading port 46 .

[0119] A fifth through hole 44 is also provided on the cover plate 47 of the top cover 4. The fifth through hole 44 is used to communicate with the storage component internal air channel 17 of the storage component 1. The storage component internal air channel 17 is connected to the reaction chamber 21. The gas in the reaction chamber 21 is connected to the outside through the storage component internal air channel 17 and the fifth through hole 44. The fifth through hole 44 can be used as a pump interface to connect an air pump. The air pump provides suction force so that the solution in the storage chamber 12 is introduced into the reaction chamber 21 through the valve 3; the air pump provides blowing force so that the solution in the reaction chamber 21 is introduced into the storage chamber 12 through the valve 3.

[0120] The storage member 1 is provided with a plurality of storage bins 12 around the groove 11, and accordingly, a plurality of puncture needles 41 are connected to the cover plate 47 of the top cover 4, and each puncture needle 41 is arranged at intervals around the midline of the groove 11, and each puncture needle 41 corresponds to a storage bin 12. Each puncture needle 41 can be connected to an annular member 45, and the outer edge of the annular member 45 is connected to the cover plate of the top cover 4 through a plurality of first ribs 421, and the inner edge of the annular member 45 can be connected to a cylindrical member 49 through a plurality of second ribs 422.

[0121] The puncture needle 41 is a hollow structure, that is, a needle inner airway 411 is provided therein, and a third through hole 412 is also provided on the puncture needle 41, and the third through hole 412 connects the needle inner airway 411 with the external atmosphere. The puncture needle 41 includes a first needle segment and a second needle segment, and the radial dimension of the second needle segment is greater than the radial dimension of the first needle segment. The second needle segment is connected to the annular member 45, and the first needle segment is configured as a sharp needle for puncturing the sealing film, and the third through hole 412 is provided in the second needle segment.

[0122] When using the microfluidic chip, pressure is applied to the annular part 45 on the top cover 4 to break the first rib 421. The annular part 45 drives the puncture needles 41 to separate from the cover plate 47 and press against the sealing film on the storage bin 12. The puncture needles 41 puncture the sealing film, and the cylindrical part 49 abuts against the circumferential side wall of the groove 11 to prevent the puncture needles 41 from moving downward excessively. At this time, the gas in the storage bin 12 is connected to the atmosphere through the needle airway 411 and the third through hole 412. After the extraction step is completed, external force continues to be applied to the annular member 45 and each puncture needle 41, the second rib 422 breaks, the annular member 45 is separated from the cylindrical member 49, the cylindrical member 49 no longer interferes with the downward movement of the puncture needle 41, and the annular member 45 and each puncture needle 41 are further pressed toward the sealing membrane under the action of external force, the second needle segment and the fourth through hole 61 are interference fit, and the second needle segment and the cover sheet 6 cooperate to block the third through hole 412 on the second needle segment, thereby sealing the storage bin 12 to prevent leakage of waste liquid in the storage bin 12.

[0123] The cover plate 6 is circular, and a fourth through hole 61, a sixth through hole 62 and a seventh through hole 63 are provided in the middle thereof. The sixth through hole 62 is aligned with the second through hole 43 on the cover plate 47, and the sixth through hole 62 is used to pass the operating part of the valve 3, or allow an external operating member to extend into the sixth through hole 62 to connect to the operating part of the valve 3. There are a plurality of fourth through holes 61, and each fourth through hole 61 corresponds to a puncture needle 41. The seventh through hole 63 is aligned with the fifth through hole 44 on the top cover 4, and is used to connect the air passage 17 in the storage member.

[0124] The radial dimension of the second needle segment of the puncture needle 41 is greater than the radial dimension of the first needle segment. When using the microfluidic chip, the first needle segment passes through the fourth through hole 61 to puncture the sealing film, and the second needle segment and the third through hole 412 are located above the cover sheet 6. After the extraction step is completed, the annular member 45 and each puncture needle 41 are further pressed toward the sealing film under the action of external force, and the second needle segment and the fourth through hole 61 have an interference fit, and the second needle segment cooperates with the cover sheet 6 to block the third through hole 412, thereby sealing the storage bin 12 to prevent leakage of waste liquid in the storage bin 12.

[0125] In summary, the puncture needle 41 on the top cover 4 is used to puncture the sealing film on the storage bin 12 to connect the storage bin 12 to the atmosphere. The cover sheet 6 is used to cooperate with the puncture needle 41 of the top cover 4 to seal the storage bin 12 after the test is completed.

[0126] like Figures 5 to 8 As shown, the storage unit 1 is cylindrical, and a groove 11 concave toward the bottom is provided in the middle of the top thereof, and a plurality of storage bins 12 are provided around the groove 11. The storage bin 12 can be used as a sample loading bin and a reagent bin 121. In this embodiment, the plurality of storage bins 12 include a sample loading bin and a plurality of reagent bins 121, the sample loading bin is used to add samples to be detected, and the reagent bin 121 is used to store reagents for biochemical reactions, the upper surface of the reagent bin 121 is sealed with a sealing film, and the lower surface is sealed, and the second end 132 of the flow channel 13 in the first storage unit is connected to the reagent bin 121 through the lowest position of the reagent bin 121. The reagent bin 121 selects a suitable bonding method according to the requirements to ensure that the reagent is packaged and sealed, and is convenient for transportation and storage. The cross section of the storage bin 12 is elliptical. The cross section of the storage bin 12 is narrow near the center line of the groove 11, and wide away from the center line of the groove 11. The size and distribution of the storage bin 12 can be adjusted according to the requirements.

[0127] The storage element 1 is provided with an internal air channel 17, and a first end of the internal air channel 17 is located between two adjacent storage bins 12. The internal air channel 17 is communicated with the reaction bin 21. A slot 14 is provided on the side of the storage element 1 for inserting the amplification element 5.

[0128] At least two first storage component inner flow channels 13 are provided in the storage component 1, and each first storage component inner flow channel 13 is connected to a corresponding storage bin 12. The first end 131 of each first storage component inner flow channel 13 passes through the bottom wall of the groove 11, and is used to communicate with the valve inner flow channel 31 of the valve 3. The second end 132 of each first storage component inner flow channel 13 is connected to the storage bin 12 through the side of the storage bin 12 adjacent to the base 2, and the second end 132 of the first storage component inner flow channel 13 is connected to the lowest part of the storage bin 12 to avoid reagent residue. And the second end 132 of the first storage component inner flow channel 13 is connected to the position of the storage bin 12 closest to the groove 11, so as to shorten the distance connected to the valve 3 and improve the detection efficiency.

[0129] A fourth storage member inner flow channel 18 is provided in the storage member 1, a first end 181 of the fourth storage member inner flow channel 18 passes through the bottom wall of the groove 11 and is located in the middle of the groove 11, and the first end 181 of the fourth storage member inner flow channel 18 is used to communicate with the valve inner flow channel 31 of the valve 3. The second end of the fourth storage member inner flow channel 18 is connected to the reaction chamber 21.

[0130] The storage element 1 is provided with a second storage element inner flow channel 15, a first end 151 of the second storage element inner flow channel 15 passes through the slot 14 for communicating with the expansion element inner flow channel 52. A second end 152 of the second storage element inner flow channel 15 passes through the groove 11 for communicating with the valve inner flow channel 31 of the valve 3.

[0131] The storage element 1 is provided with a third storage element inner flow channel 16, a first end 161 of the third storage element inner flow channel 16 passes through the slot 14 for communicating with the expansion element inner air channel 53. A second end 162 of the third storage element inner flow channel 16 passes through the groove 11 for communicating with the valve inner air channel 34 of the valve 3.

[0132] A first buckle 141 is provided in the slot 14 for mating and connecting with the second buckle 54 on the expansion component 5 .

[0133] like Fig. 9 and Fig.10 As shown, the valve 3 is used to control the closing of the liquid circuit and the communication between the chambers. The valve 3 includes a rotor 32 and a valve cover 33.

[0134] The valve cover 33 is used to connect the circumferential side wall of the groove 11. The valve cover 33 is provided with a first through hole 331. The operating part of the rotor 32 passes through the first through hole 331. The operating part of the rotor 32 is configured to be connected to an external operating member. The valve cover 33 is provided with a plurality of connection blocks connected to the circumferential side wall of the groove 11.

[0135] The rotor 32 is rotatably disposed in the groove 11. The rotor 21 includes a valve seat body 3211, a valve stem 322 and a first gasket 3212. The first gasket 3212 has the same bottom shape as the valve seat body 3211, both of which are circular. The valve seat body 3211 and the first gasket 3212 are fixedly arranged. The inner flow channel 31 is formed in the combined structure of the valve seat body 3211 and the first gasket 3212.

[0136] The radial dimension of the valve stem 322 is smaller than the radial dimension of the valve seat body 3211 . One end of the valve stem 322 is fixedly connected to the valve seat body 3211 . The other end of the valve stem 322 is an operating part for passing through the first through hole 331 and connecting to an external operating member.

[0137] An inner valve flow channel 31 and an inner valve air channel 34 are provided in the valve 3 .

[0138] The first end 311 of the valve internal flow channel 31 is located in the middle of the valve 3 and is aligned and connected with the first end 181 of the fourth storage component internal flow channel 18 on the groove 11. The valve internal flow channel 31 is always connected with the reaction chamber 21 through the fourth storage component internal flow channel 18. The second end 312 of the valve internal flow channel 31 can be selectively connected with any first storage component internal flow channel 13 or second storage component internal flow channel 15 during the rotation of the rotor 32. The second end 312 of the valve internal flow channel 31 is close to the outer edge of the valve 3.

[0139] When the second end 312 of the flow channel 31 in the valve is connected to the flow channel 15 in the second storage component, the first end of the air channel 34 in the valve is connected to the second end 162 of the flow channel 16 in the third storage component, the second end of the air channel 34 in the valve is connected to the first end 131 of the flow channel 13 in the first storage component, and the second end 132 of the flow channel 13 in the first storage component is connected to a storage bin 12.

[0140] The operating part of the valve stem 322 is connected through an external operating part, and the valve stem 322 is rotated, thereby driving the valve seat body 3211 and the first gasket 3212 to rotate, so as to selectively connect the second end 312 of the valve internal flow channel 31 with a first storage component internal flow channel 13 or with a second storage component internal flow channel 15, thereby completing the liquid flow transfer during the detection process.

[0141] Optionally, the operating portion of the valve stem 322 is configured as a hexagonal structure.

[0142] A boss 324 is provided on the circumference of the valve seat body 3211 . The boss 324 has an arc-shaped outer contour to reduce friction between the circumference of the valve seat body 3211 and the circumferential side wall of the groove 11 when the valve seat body 3211 rotates relative to the groove 11 .

[0143] like Figures 11 to 14 As shown, the base 2 includes a bottom plate 22 , a support member 23 and a positioning member 24 .

[0144] The surface of the bottom plate 22 is circular, and a positioning protrusion 27 is provided on the bottom plate 22 . The positioning protrusion 27 is used to connect the storage element 1 .

[0145] The support member 23 is arranged below the chassis 22, and is used to support the chassis 22 and the entire microfluidic chip. The support member 23 serves as a supporting structure of the microfluidic chip, so that the microfluidic chip can be placed stably. A positioning groove 28 is also provided at the bottom of the support member 23, and the positioning groove 28 is used to cooperate with the placement platform on the detection device to complete the initial positioning of the microfluidic chip. A clamping groove 25 is formed between the support member 23 and the chassis 22. When the microfluidic chip is pushed into the detection device, the clamping groove 25 is used to cooperate with the structure on the detection device for positioning, thereby fixing the microfluidic chip, avoiding detection errors caused by the movement of the microfluidic chip during the detection process, and improving detection consistency.

[0146] The reaction chamber 21 is arranged at the bottom of the chassis 22, protruding downward, and is a spherical crown structure. The structure can be coupled with the ultrasonic head to quickly reach resonance, assisting the sample lysis and mixing with the magnetic beads. The chassis 22 is provided with a chassis inner flow channel connected to the reaction chamber 21. The first end 261 of the chassis inner flow channel is located in the middle of the chassis 22, and the second end 262 of the chassis inner flow channel is connected to the reaction chamber 21. The first end 261 of the chassis inner flow channel is connected to the fourth storage unit inner flow channel 18, and is aligned with the first end 311 of the valve inner flow channel 31, so that the valve inner flow channel 31 is always connected to the reaction chamber 21. The second end 262 of the chassis inner flow channel is connected to the reaction chamber 21 through the lowest part of the reaction chamber 21 to avoid the formation of a dead zone that causes the reagent to be unable to be discharged.

[0147] The positioning member 24 is disposed on the supporting member 23 and is used for positioning the microfluidic chip when it is installed on the detection device.

[0148] The top cover 4 is fixed to one end of the storage element 1 with the groove 11, the base 2 is fixed to one end of the storage element 1 away from the top cover 4, and the valve 3 is arranged in the groove 11. The top cover 4, the storage element 1, the base 2 and the valve 3 form the main structure of the microfluidic chip.

[0149] like Figures 15 to 17 As shown, the amplification component 5 is a thin sheet structure for realizing rapid temperature rise and fall amplification. The amplification component 5 is pluggable and detachable from the slot 14 of the storage component 1. The amplification component 5 can be separated from the main structure of the microfluidic chip, and can be produced and bonded using a material different from the main structure.

[0150] The amplification component 5 is provided with an amplification chamber 51, which is in the shape of a thin sheet so that it can have a larger contact surface and thermal conductivity with the heat source. The amplification component 5 is provided with an amplification component inner flow channel 52 connected to the amplification chamber 51, and the amplification component inner flow channel 52 is connected to the first end 151 of the second storage component inner flow channel 15. The amplification component 5 is provided with an amplification component inner air channel 53 connected to the amplification chamber 51, and the amplification component inner air channel 53 is connected to the first end 161 of the third storage component inner flow channel 16.

[0151] The connection between the main structure of the microfluidic chip and the amplification component 5 can be fixed with the soft rubber second gasket 7 by secondary injection molding or bonding, so as to ensure the airtightness of the flow channel 52 and the air channel 53 in the amplification component at the connection.

[0152] The cross-sections of the first buckle 141 set in the slot 14 and the second buckle 54 set on the amplification component 5 can both be triangular. After the amplification component 5 is inserted into the slot 14, the first buckle 141 and the second buckle 54 limit each other to prevent the amplification component 5 from being pulled out of the slot 14.

[0153] Some embodiments further provide a microfluidic chip detection system, which includes a detection device and the above-mentioned microfluidic chip, the detection device includes an operating table for accommodating the microfluidic chip, and an operating member for operating the valve 3.

[0154] The microfluidic chip detection system provided by the embodiment of the present disclosure has low requirements for operators. It only requires adding the sample to be tested, placing the microfluidic chip into the detection device, and clicking the start button to start the detection process including extraction and amplification.

[0155] The following describes the detection process of the microfluidic chip:

[0156] According to the test items, select the microfluidic chip loaded with the corresponding reagents, and inject the sample to be tested into the sample chamber of the microfluidic chip to complete the preliminary preparations.

[0157] The operator pays attention to the positioning structure of the base 2 of the microfluidic chip corresponding to the detection device, and places it flat on the tray of the detection device to complete the initial positioning. After clicking the start button, the tray enters the working area of ​​the detection device, and the clamping groove 25 of the base 2 cooperates with the structure on the detection device to clamp and fix the microfluidic chip.

[0158] The detection process begins, the air pump is connected to the air pump interface on the microfluidic chip, the puncture needle 41 on the top cover 4 of the microfluidic chip is subjected to downward pressure, the first rib 421 breaks, the puncture needle 41 is separated from the top cover 4, and the sealing film on the storage bin 12 is punctured. The storage bin 12 is connected to the air through the needle airway 411 and the third through hole 412 in the puncture needle 41, preparing for the release of the reagent.

[0159] The valve 3 is rotated to connect a storage chamber 12 and a reaction chamber 21 respectively, and a power source is provided by an external air pump to sequentially complete the extraction of reagents required for each extraction step.

[0160] This embodiment uses the magnetic bead method for nucleic acid extraction. After the reagents in the storage bin 12 are extracted and enter the reaction bin 21, the ultrasonic head will couple and resonate with the reaction bin 21. The spherical crown structure of the reaction bin 21 can provide relatively good support force to avoid wall deformation during the ultrasonic process, and the contact surface also has good consistency. Under the action of ultrasound, the vibration of the wall drives the reagents and magnetic beads in the reaction bin 21 to agitate, and the auxiliary lysis of the sample and the mixing of the magnetic beads can be completed within a few seconds. The waste liquid after the reaction will be transferred from the reaction bin 21 back to the storage bin 12, and then sealed by rotating the valve 3.

[0161] After extracting reagents in sequence and reacting in the reaction chamber 21, a purified extraction product is finally obtained, and the purified extraction product is transferred to the amplification chamber 51, and then the amplification chamber 51 is sealed by rotating the valve 3, waiting for the amplification module of the detection equipment to perform rapid amplification and multi-channel optical detection of the sample.

[0162] After the detection is completed, the downward pressure module in the detection equipment will act on the puncture needle 41 on the top cover 4 of the microfluidic chip again. The downward pressure will break the second rib 422, and the puncture needle 41 will continue to move downward. The second needle segment of the puncture needle 41 forms an interference fit with the cover plate 6. The cover plate 6 covers the third through hole 412 on the second needle segment, so that the storage bin 12 is isolated from the atmosphere to prevent leakage of reaction waste liquid in the storage bin 12.

[0163] At this point, the microfluidic chip detection process is complete. You can click the exit button to remove the pressure, take out the microfluidic chip and start the next set of detection.

[0164] The flow channel in the present disclosure can be used for liquid transmission and can also be used for gas transmission. Similarly, the gas channel can be used for gas transmission and can also be used for liquid transmission.

[0165] Based on the above-mentioned embodiments of the present invention, unless explicitly denied, the technical features of one embodiment may be beneficially combined with one or more other embodiments.

[0166] Although some specific embodiments of the present invention have been described in detail by way of examples, it should be understood by those skilled in the art that the above examples are for illustration only and are not intended to limit the scope of the present invention. It should be understood by those skilled in the art that the above embodiments may be modified or some technical features may be replaced by equivalents without departing from the scope and spirit of the present invention. The scope of the present invention is defined by the appended claims.

Claims

1. A microfluidic chip, characterized in that: include: A storage element (1) having a groove (11) formed thereon, and at least two storage bins (12) being arranged around the groove (11); a base (2), arranged at an end of the storage element (1) away from the groove (11), the base (2) being provided with a reaction chamber (21); and a valve (3) disposed in the groove (11), the valve (3) being configured to operably connect any one of the at least two storage bins (12) to the reaction bin (21); The storage element (1) is provided with at least two first storage element internal flow channels (13), each first storage element internal flow channel (13) correspondingly connected to a storage bin (12), the valve (3) is provided with a valve internal flow channel (31) connected to the reaction bin (21), and the valve (3) is configured to operably connect the valve internal flow channel (31) to any first storage element internal flow channel (13); The microfluidic chip further comprises a sealing film, the at least two storage chambers (12) comprise a reagent chamber (121), the sealing film is configured to seal the reagent chamber (121), the microfluidic chip further comprises a top cover (4) and a puncture needle (41), the top cover (4) being arranged at one end of the storage element (1) provided with the groove (11), the puncture needle (41) being connected to the top cover (4), and the puncture needle (41) being configured to press against the sealing film under the action of an external force so as to puncture the sealing film; An inner needle airway (411) is provided in the puncture needle (41), and a third through hole (412) is provided near the portion where the puncture needle (41) is connected to the top cover (4) and connects the outside of the puncture needle (41) and the inner needle airway (411); The microfluidic chip further comprises a cover sheet (6), wherein the cover sheet (6) is arranged inside the top cover (4), and a fourth through hole (61) is provided on the cover sheet (6) for allowing the puncture needle (41) to pass through. The puncture needle (41) is configured to be pressed toward the sealing film under the action of an external force, and to continue to be pressed toward the sealing film after puncturing the sealing film, so that the third through hole (412) is sealed by the cover sheet (6).

2. The microfluidic chip according to claim 1, characterized in that: The first end (131) of the flow channel (13) in each first storage element passes through the bottom wall of the groove (11), and the valve (3) is configured to operably connect the flow channel (31) in the valve with the first end (131) of the flow channel (13) in the first storage element, and the second end (132) of the flow channel (13) in each first storage element is connected to the storage bin (12) via a side of the storage bin (12) adjacent to the base (2).

3. The microfluidic chip according to claim 2, characterized in that: The second end (132) of the flow channel (13) in the first storage element is connected to the lowest part of the storage bin (12).

4. The microfluidic chip according to claim 2, characterized in that: The first end (311) and the second end (312) of the flow channel (31) in the valve both pass through an end of the bottom wall of the valve (3) adjacent to the groove (11); the first end (311) of the flow channel (31) in the valve is connected to the reaction chamber (21); the second end (312) of the flow channel (31) in the valve is operably connected to any one of the flow channels (13) in the first storage element; the first end (311) of the flow channel (31) in the valve is located in the middle of the valve (3); and the second end (312) of the flow channel (31) in the valve is close to the outer edge of the valve (3).

5. The microfluidic chip according to claim 1, characterized in that: The valve (3) comprises: a rotor (32) rotatably disposed in the groove (11), the rotor (32) comprising a valve seat (321) and a valve stem (322), the valve stem (322) being connected to the valve seat (321); and The valve cover (33) is connected to the circumferential side wall of the groove (11) and abuts against the valve seat (321) to limit the valve seat (321) between the valve cover (33) and the bottom wall of the groove (11); the valve cover (33) is provided with a first through hole (331); the operating portion of the valve stem (322) passes through the first through hole (331); and the operating portion of the valve stem (322) is configured to be connected to an external operating member.

6. The microfluidic chip according to claim 5, characterized in that: The valve cover (33) abuts against a circumferential edge of the valve seat (321).

7. The microfluidic chip according to claim 1, characterized in that: The top cover (4) comprises a first rib (421), the puncture needle (41) is connected to the first rib (421), and the first rib (421) is configured to be disconnected under the action of an external force so that the puncture needle (41) is separated from the top cover (4) and pressed toward the sealing membrane.

8. The microfluidic chip according to claim 1, characterized in that: A second through hole (43) is provided in the middle of the top cover (4), and the second through hole (43) is configured to allow an external operating member to pass through so as to operate the valve (3).

9. The microfluidic chip according to claim 1, characterized in that: The reaction chamber (21) protrudes towards a side away from the storage element (1).

10. The microfluidic chip according to claim 9, characterized in that: The reaction chamber (21) is a spherical crown structure.

11. The microfluidic chip according to claim 1, characterized in that: It also includes an amplification component (5), the amplification component (5) is provided with an amplification chamber (51), a side portion of the storage component (1) is provided with a slot (14), the slot (14) is located between two adjacent storage chambers (12), the amplification component (5) is plugged into the slot (14), and the valve (3) is configured to operably connect the reaction chamber (21) with the amplification chamber (51).

12. The microfluidic chip according to claim 11, characterized in that: The storage element (1) is provided with a second storage element inner flow channel (15), the first end (151) of the second storage element inner flow channel (15) passes through the slot (14), the second end (152) of the second storage element inner flow channel (15) passes through the groove (11), the amplification element (5) is provided with an amplification element inner flow channel (52) connected to the amplification chamber (51), the amplification element inner flow channel (52) is connected to the first end (151) of the second storage element inner flow channel (15), and the valve (3) is configured to operably connect the second end (152) of the second storage element inner flow channel (15) to guide the solution in the reaction chamber (21) to the amplification chamber (51) through the second storage element inner flow channel (15) and the amplification element inner flow channel (52).

13. The microfluidic chip according to claim 12, characterized in that: The storage component (1) is provided with a third storage component inner flow channel (16), the first end (161) of the third storage component inner flow channel (16) passes through the slot (14), the second end (162) of the third storage component inner flow channel (16) passes through the groove (11), the amplification component (5) is provided with an amplification component inner air channel (53) connected to the amplification chamber (51), the amplification component inner air channel (53) is connected to the first end (161) of the third storage component inner flow channel (16), and the valve (3) is configured to operably connect to the second end (162) of the third storage component inner flow channel (16) so as to guide the gas in the amplification chamber (51) to a storage chamber (12) through the amplification component inner air channel (53) and the third storage component inner flow channel (16).

14. The microfluidic chip according to claim 11, characterized in that: The valve (3) is provided with an intra-valve flow channel (31) connected to the reaction chamber (21), and the valve (3) is also provided with an intra-valve air channel (34). The valve (3) is configured to operably connect the intra-valve flow channel (31) to the reaction chamber (21) and the amplification chamber (51), and to connect the intra-valve air channel (34) to the amplification chamber (51) and a storage chamber (12).

15. The microfluidic chip according to claim 1, characterized in that: The storage component (1) is provided with an internal air passage (17), the internal air passage (17) being connected to the reaction chamber (21), and the internal air passage (17) being configured to be connected to an external air pump.

16. The microfluidic chip according to claim 15, characterized in that: The first end (171) of the air channel (17) in the storage element passes through one end of the storage element (1) where the groove (11) is provided, and the first end (171) of the air channel (17) in the storage element is located between two adjacent storage bins (12).

17. The microfluidic chip according to claim 1, characterized in that: The top cover (4) is fixedly arranged at one end of the storage component (1) where the groove (11) is provided, and the base (2) is fixedly arranged at one end of the storage component (1) away from the top cover (4).

18. A microfluidic chip detection system, characterized in that: The invention comprises a detection device and a microfluidic chip according to any one of claims 1 to 17, wherein the detection device comprises an operating table for accommodating the microfluidic chip and an operating member for operating the valve (3).

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