A plant antibacterial spray composition and preparation method thereof

Abstract

The invention discloses a plant antibacterial spray composition and a preparation method thereof. The plant antibacterial spray composition is prepared by mixing water extracts of different plant tissues with organic solvent extracts with the aid of an emulsifier. The plant tissues include but are not limited to one or more of Artemisia annua leaves, Magnolia officinalis bark, Myrtle fruit, Sophora flavescens root, Honeysuckle flower, Coptis root, and Scutellaria baicalensis root. The plant antibacterial spray composition of the invention has a simple preparation process and good antibacterial effect.

Description

Technical Field

[0001] The invention belongs to the field of comprehensive resource utilization, and specifically relates to a plant antibacterial spray composition with bactericidal and anti-inflammatory effects and a preparation method thereof. Background Art

[0002] Traditional Chinese medicine generally uses water extraction to extract the active ingredients in Chinese medicinal materials. Water extraction is also called decoction, which refers to a method of using water as a solvent to soak or (and) heat and boil to extract the active ingredients of plants or Chinese medicinal materials. Feng Ling et al. reported the in vitro antibacterial effect of 15 Chinese herbal decoctions on fox-derived O1 extraintestinal pathogenic Escherichia coli. However, the system with water as the solvent inevitably causes the loss of fat-soluble substances. How to fully utilize the water-soluble and fat-soluble metabolites of Chinese medicine is an urgent problem that still needs to be solved in the industry. Summary of the invention

[0003] The object of the present invention is to provide a plant antibacterial spray composition with good bactericidal effect and a preparation method thereof in view of the deficiencies of the prior art.

[0004] In order to achieve the above object, the present invention adopts the following technical solution:

[0005] A method for preparing a plant antibacterial spray composition, characterized in that it comprises the following steps:

[0006] S1 Preparation of Sample A

[0007] The plant tissues are sorted, washed, and drained, and then placed in an oven for high-temperature sterilization, and then dried to constant weight. The dried plant tissues are crushed into plant tissue powder using a high-speed tissue crusher. After sieving, the plant tissue powder is weighed and extracted in water. After high-speed centrifugation, the precipitate is discarded, and the supernatant is filtered through a clean filter for later use, thereby obtaining sample A;

[0008] Preparation of S2 Sample B

[0009] The plant tissues are sorted, washed, drained, and then placed in an oven for high-temperature sterilization, and then dried to constant weight. The dried plant tissues are crushed into plant tissue powder using a high-speed tissue crusher. After sieving, the plant tissue powder is weighed and extracted with ethanol reflux. The filtrate is concentrated under reduced pressure to an extract, then dissolved in an organic solvent, filtered through a filter, and set aside to obtain sample B.

[0010] Preparation of S3 Sample C

[0011] Sample B and the emulsifier were magnetically stirred at room temperature at a speed of 1000 r / min for 1 h. During the stirring process, sample A was slowly added dropwise. Sample C was obtained when the system changed from turbid to clear.

[0012] Preferably, the high temperature in steps S1 and S2 is 80-150° C., the fixing time is 5-30 min, the weight ratio of plant powder to water in step S1 is 1:5, the organic solvent in step S2 includes but is not limited to glycerol, propylene glycol, isopropanol, ethanol, propionic acid, etc., and the ratio of plant powder to organic solvent is 1:5.

[0013] Preferably, in steps S1 and S2, 20 mesh sieving is used.

[0014] Preferably, in step S1, the temperature of water is 90° C. and the extraction time is 3 hours.

[0015] Preferably, the pore size of the filter in steps S1 and S2 is 0.45 μm.

[0016] Preferably, the ethanol reflux extraction time in step S2 is 3 hours.

[0017] Preferably, as a preferred embodiment, the emulsifier in step S3 is selected from one or more of Tween 20, Tween 60, Tween 80, Span 60 and Span 80.

[0018] Preferably, as a preferred embodiment, the usage ratio of sample A, sample B and emulsifier in step S3 is 1:1:2.

[0019] Preferably, as a preferred embodiment, the plant tissue is selected from one or more of Artemisia annua leaf, Magnolia officinalis bark, Myrtle fruit, Sophora flavescens root, Honeysuckle flower, Coptis chinensis root, and Scutellaria baicalensis root.

[0020] Preferably, as a better embodiment, sample A prepared from the first plant tissue is mixed with sample B prepared from the second plant tissue to prepare a new mixed sample. In some cases, this mixing can achieve unexpected technical effects, and the bactericidal activity of the mixed sample can be improved. Although the mechanism of action is not clear and unpredictable, specific compositions are particularly effective.

[0021] Preferably, as a preferred embodiment, sample A is an Artemisia annua leaf sample, and sample B is a Myrtle sample.

[0022] Another object of the present invention is to provide an application of a plant antibacterial spray composition with good bactericidal effect, wherein the plant antibacterial spray composition is particularly used for inhibiting harmful fungi and / or bacteria.

[0023] Preferably, as a preferred embodiment, the types of harmful fungi and / or bacteria include but are not limited to Candida albicans and Staphylococcus aureus.

[0024] Compared with the prior art, the present invention has the following beneficial effects:

[0025] 1. The present invention mixes the plant extract extracted with water and the plant extract extracted with an organic solvent by means of an emulsifier, which can greatly improve the antibacterial effect.

[0026] 2. The present invention mixes different plant extracts, especially by using water extracts of different plants in combination with organic solvent extracts of another plant, which, in some cases, exhibits a certain synergistic effect. DETAILED DESCRIPTION

[0027] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work belong to the scope of protection of the present invention. The following specific implementation methods further describe the present invention.

[0028] 1. Preparation of different extract samples

[0029] 1. Preparation of Artemisia annua samples

[0030] A: Preparation of Artemisia annua leaf sample A

[0031] Artemisia aannua leaves were sorted, washed, drained, and placed in an oven at 105°C for 10 minutes, then dried at 60°C to constant weight. The dried Artemisia aannua leaves were crushed with a high-speed tissue crusher, sieved through a 20-mesh sieve, and 10 g of Artemisia aannua leaf powder was weighed and extracted in water at 90°C for 3 hours at a solid-liquid ratio of 1:5. The mixture was centrifuged at 1500 r / min for 3 minutes and the precipitate was discarded. 50 ml of the supernatant was filtered through a filter with a pore size of 0.45 μm and set aside, marked as Artemisia aannua leaf sample A.

[0032] B: Preparation of Artemisia annua leaf sample B

[0033] The green song leaves were sorted, washed, drained, and placed in an oven at 105°C for 10 minutes, then dried at 60°C to constant weight. The dried green song leaves were crushed with a high-speed tissue crusher, sieved through a 20-mesh sieve, and 10 g of green song leaf powder was extracted with ethanol reflux for 3 h. The filtrate was concentrated under reduced pressure to an extract and then dissolved in 50 ml of isopropanol. It was filtered through a filter with a pore size of 0.45 μm and set aside, labeled as green song leaf sample B.

[0034] C: Preparation of Artemisia annua leaf sample C

[0035] Take 10 ml of Song leaf sample B and 20 ml of Tween 20 and stir magnetically at 1000 r / min at room temperature for 1 h. During the stirring process, slowly add 10 ml of Song leaf sample A drop by drop until the system changes from turbid to clear. Label it as Song leaf sample C.

[0036] D: Preparation of Artemisia annua leaf sample D

[0037] Take 10 ml of Song leaf sample A and 10 ml of Song leaf sample B and mix them by physical vibration for 1 hour, and mark them as Song leaf sample D.

[0038] 2. Preparation of Magnolia officinalis bark samples

[0039] A: Preparation of Magnolia officinalis bark sample A

[0040] The bark of Magnolia officinalis was sorted, washed, drained, and then placed in an oven at 105°C for 10 minutes, then dried at 60°C to constant weight. The dried bark of Magnolia officinalis was crushed with a high-speed tissue crusher, sieved through 20 mesh, and 10 g of Magnolia officinalis bark powder was weighed and extracted in water at 90°C for 3 h at a solid-liquid ratio of 1:5. The powder was centrifuged at 1500 r / min for 3 min and the precipitate was discarded. 50 ml of the supernatant was filtered through a filter with a pore size of 0.45 μm and set aside, marked as Magnolia officinalis bark sample A.

[0041] B: Preparation of Magnolia officinalis bark sample B

[0042] The bark of Magnolia officinalis was sorted, washed, drained, and then placed in an oven at 105°C for 10 minutes, then dried at 60°C to constant weight. The dried bark of Magnolia officinalis was crushed with a high-speed tissue crusher, sieved through 20 mesh, and 10 g of the bark powder was extracted with ethanol reflux for 3 hours. The filtrate was concentrated under reduced pressure to an extract and then dissolved in 50 ml of isopropanol. It was filtered through a filter with a pore size of 0.45 μm and set aside for later use, labeled as Magnolia officinalis bark sample B.

[0043] C: Preparation of Magnolia officinalis bark sample C

[0044] Take 10 ml of Magnolia officinalis bark sample B and 20 ml of Tween 20 and stir them magnetically at 1000 r / min for 1 hour at room temperature. During the stirring process, slowly add 10 ml of Magnolia officinalis bark sample A drop by drop until the system changes from turbid to clear. Label it as Magnolia officinalis bark sample C.

[0045] D: Preparation of Magnolia officinalis bark sample D

[0046] Take 10 ml of Magnolia officinalis bark sample A and 10 ml of Magnolia officinalis bark sample B and mix them by physical shaking for 1 hour. Label it as Magnolia officinalis bark sample D.

[0047] 3. Myrtle fruit sample preparation

[0048] A: Preparation of Myrtle Fruit Sample A

[0049] The myrtle (RHODOMYRTUSTOMENTOSA) fruits were sorted, washed, drained, and placed in an oven at 105°C for 10 minutes, then dried at 60°C to constant weight. The dried myrtle fruits were crushed with a high-speed tissue crusher, sieved through 20 meshes, and 10 g of myrtle fruit powder was weighed and extracted with water at 90°C for 3 hours at a solid-liquid ratio of 1:5. The precipitate was discarded after centrifugation at 1500 r / min for 3 minutes, and 50 ml of the supernatant was filtered through a filter with a pore size of 0.45 μm for later use, which was labeled as myrtle fruit sample A.

[0050] B: Preparation of Myrtle Fruit Sample B

[0051] The myrtle fruit was sorted, washed, drained, and placed in an oven at 105°C for 10 minutes, then dried at 60°C to constant weight. The dried myrtle fruit was crushed with a high-speed tissue crusher, sieved through 20 mesh, and 10 g of myrtle fruit powder was extracted with ethanol reflux for 3 hours. The filtrate was concentrated under reduced pressure to an extract and then dissolved in 50 ml of isopropanol. It was filtered through a filter with a pore size of 0.45 μm and set aside, labeled as myrtle fruit sample B.

[0052] C: Preparation of Myrtle Fruit Sample C

[0053] Take 10 ml of myrtle fruit sample B and 20 ml of Tween 20 and stir them magnetically at 1000 r / min for 1 hour at room temperature. During the stirring process, slowly add 10 ml of myrtle fruit sample A drop by drop until the system changes from turbid to clear. Label it as myrtle fruit sample C.

[0054] D: Preparation of Myrtle Fruit Sample D

[0055] Take 10 ml of Myrtle Fruit Sample A and 10 ml of Myrtle Fruit Sample B and mix them by physical shaking for 1 hour. Label it as Myrtle Fruit Sample D.

[0056] 4. Preparation of Sophora flavescens root samples

[0057] A: Preparation of Sophora flavescens root sample A

[0058] The roots of Sophora flavescens (SOP HO RAAN GUSTI FO LIA) were sorted, washed, drained, and then placed in an oven at 105°C for 10 minutes to fix the greenness, and then dried at 60°C to constant weight. The dried roots were crushed with a high-speed tissue crusher, sieved through 20 meshes, and 10 g of the root powder was weighed and extracted in water at 90°C for 3 h at a solid-liquid ratio of 1:5. The precipitate was discarded after centrifugation at 1500 r / min for 3 min, and 50 ml of the supernatant was filtered through a filter with a pore size of 0.45 μm for later use, which was marked as Sophora flavescens root sample A.

[0059] B: Preparation of Sophora flavescens root sample B

[0060] The roots of Sophora flavescens were sorted, washed, drained, and placed in an oven at 105°C for 10 minutes, then dried at 60°C to constant weight. The dried roots were crushed with a high-speed tissue crusher, sieved through 20 mesh, and 10 g of Sophora flavescens root powder was extracted with ethanol reflux for 3 hours. The filtrate was concentrated under reduced pressure to an extract and then dissolved in 50 ml of isopropanol. It was filtered through a filter with a pore size of 0.45 μm and set aside for later use, labeled as Sophora flavescens root sample B.

[0061] C: Preparation of Sophora flavescens root sample C

[0062] Take 10 ml of Sophora flavescens root sample B and 20 ml of Tween 20 and stir them magnetically at 1000 r / min for 1 hour at room temperature. During the stirring process, slowly add 10 ml of Sophora flavescens root sample A drop by drop until the system changes from turbid to clear. Label it as Sophora flavescens root sample C.

[0063] D: Preparation of Sophora flavescens root sample D

[0064] Take 10 ml of Sophora flavescens root sample A and 10 ml of Sophora flavescens root sample B and mix them by physical vibration for 1 hour. Label them as Sophora flavescens root sample D.

[0065] 5. Preparation of Honeysuckle Samples

[0066] A: Preparation of Honeysuckle Sample A

[0067] The flowers of honeysuckle (LONICE RAJAPONICA) were sorted, washed, drained, and then placed in an oven at 105°C for 10 minutes to fix green, and then dried at 60°C to constant weight. The dried flowers were crushed with a high-speed tissue crusher, sieved through 20 mesh, and 10 g of honeysuckle flower powder was weighed and extracted in water at 90°C for 3 h at a solid-liquid ratio of 1:5. The precipitate was discarded after centrifugation at 1500 r / min for 3 min, and 50 ml of the supernatant was filtered through a filter with a pore size of 0.45 μm for use, which was marked as honeysuckle sample A.

[0068] B: Preparation of Honeysuckle Sample B

[0069] After sorting, washing and draining, honeysuckle flowers were placed in an oven at 105°C for 10 minutes, and then dried at 60°C to constant weight. The dried honeysuckle flowers were crushed with a high-speed tissue crusher, sieved through 20 meshes, and 10g of honeysuckle powder was extracted with ethanol reflux for 3h. The filtrate was concentrated under reduced pressure to an extract and then dissolved in 50ml of isopropanol. It was filtered through a filter with a pore size of 0.45μm and set aside for later use, marked as honeysuckle sample B.

[0070] C: Preparation of Honeysuckle Sample C

[0071] Take 10 ml of honeysuckle sample B and 20 ml of Tween 20 and stir them magnetically at 1000 r / min for 1 hour at room temperature. During the stirring process, slowly add 10 ml of honeysuckle sample A drop by drop until the system changes from turbid to clear. Label it as honeysuckle sample C.

[0072] D: Preparation of Honeysuckle Sample D

[0073] Take 10 ml of Honeysuckle Sample A and 10 ml of Honeysuckle Sample B and mix them by physical shaking for 1 hour. Label it as Honeysuckle Sample D.

[0074] 6. Preparation of Coptis chinensis root samples

[0075] A: Preparation of Coptis chinensis root sample A

[0076] The roots of Coptis chinensis were sorted, washed, drained, and placed in an oven at 105°C for 10 minutes, then dried at 60°C to constant weight. The dried roots were crushed with a high-speed tissue crusher, sieved through 20 mesh, and 10 g of the root powder was weighed and extracted in water at 90°C for 3 h at a solid-liquid ratio of 1:5. The precipitate was discarded after centrifugation at 1500 r / min for 3 min, and 50 ml of the supernatant was filtered through a filter with a pore size of 0.45 μm for later use, which was labeled as Coptis chinensis root sample A.

[0077] B: Preparation of Coptis chinensis root sample B

[0078] The roots of Coptis chinensis were sorted, washed, drained, and placed in an oven at 105°C for 10 minutes, then dried at 60°C to constant weight. The dried roots were crushed with a high-speed tissue crusher, sieved through 20 mesh, and 10 g of the root powder was extracted with ethanol reflux for 3 h. The filtrate was concentrated under reduced pressure to an extract and then dissolved in 50 ml of isopropanol. It was filtered through a filter with a pore size of 0.45 μm and set aside for later use, labeled as Coptis chinensis root sample B.

[0079] C: Preparation of Coptis chinensis root sample C

[0080] Take 10 ml of Coptis chinensis root sample B and 20 ml of Tween 20 and stir them magnetically at 1000 r / min for 1 hour at room temperature. During the stirring process, slowly add 10 ml of Coptis chinensis root sample A drop by drop until the system changes from turbid to clear. Label it as Coptis chinensis root sample C.

[0081] D: Preparation of Coptis chinensis root sample D

[0082] Take 10 ml of Coptis chinensis root sample A and 10 ml of Coptis chinensis root sample B and mix them by physical vibration for 1 hour. Label it as Coptis chinensis root sample D.

[0083] 7. Preparation of Scutellaria baicalensis root samples

[0084] A: Preparation of Scutellaria baicalensis Root Sample A

[0085] The roots of Scutellaria baicalensis were sorted, washed, drained, and then placed in an oven at 105°C for 10 minutes, then dried at 60°C to constant weight. The dried roots were crushed with a high-speed tissue crusher, sieved through a 20-mesh sieve, and 10 g of the root powder was weighed and extracted in water at 90°C for 3 hours at a solid-liquid ratio of 1:5. The precipitate was discarded after centrifugation at 1500 r / min for 3 minutes, and 50 ml of the supernatant was filtered through a filter with a pore size of 0.45 μm and set aside, marked as Scutellaria baicalensis root sample A.

[0086] B: Preparation of Scutellaria baicalensis root sample B

[0087] The root of Scutellaria baicalensis was sorted, washed, drained, and then placed in an oven at 105°C for 10 minutes, then dried at 60°C to constant weight. The dried root of Scutellaria baicalensis was crushed with a high-speed tissue crusher, sieved through 20 mesh, and 10 g of the root powder was extracted with ethanol reflux for 3 hours. The filtrate was concentrated under reduced pressure to an extract and then dissolved in 50 ml of isopropanol. It was filtered through a filter with a pore size of 0.45 μm and set aside, labeled as Scutellaria baicalensis root sample B.

[0088] C: Preparation of Scutellaria baicalensis root sample C

[0089] Take 10 ml of Scutellaria root sample B and 20 ml of Tween 20 and stir them magnetically at 1000 r / min for 1 hour at room temperature. During the stirring process, slowly add 10 ml of Scutellaria root sample A drop by drop until the system changes from turbid to clear. Label it as Scutellaria root sample C.

[0090] D: Preparation of Scutellaria baicalensis root sample D

[0091] Take 10 ml of Scutellaria root sample A and 10 ml of Scutellaria root sample B and mix them by physical vibration for 1 hour. Label it as Scutellaria root sample D.

[0092] 2. Preparation of mixed samples

[0093] 1. Preparation of mixed sample A

[0094] Take 10 ml of Myrtle Sample B and 20 ml of Tween 20 and stir them magnetically at 1000 r / min for 1 hour at room temperature. During the stirring process, slowly add 10 ml of Song Leaf Sample A drop by drop until the system changes from turbid to clear. Label it as Mixed Sample A.

[0095] 2. Preparation of mixed sample B

[0096] Take 10 ml of Qing Song leaf sample B and 20 ml of Tween 20 and stir them magnetically at 1000 r / min for 1 hour at room temperature. During the stirring process, slowly add 10 ml of Myrtle sample A drop by drop until the system changes from turbid to clear. Label it as mixed sample B.

[0097] 3. Preparation of mixed sample C

[0098] Take 10 ml of Sophora flavescens root sample B and 20 ml of Tween 20 and stir them magnetically at 1000 r / min for 1 hour at room temperature. During the stirring process, slowly add 10 ml of Magnolia officinalis bark sample A drop by drop until the system changes from turbid to clear. Label it as mixed sample C.

[0099] 4. Preparation of mixed sample D

[0100] Take 10 ml of Magnolia officinalis bark sample B and 20 ml of Tween 20 and stir them magnetically at 1000 r / min for 1 hour at room temperature. During the stirring process, slowly add 10 ml of Sophora flavescens root sample A drop by drop until the system changes from turbid to clear. Label it as mixed sample D.

[0101] 5. Preparation of mixed sample E

[0102] Take 10 ml of Scutellaria baicalensis root sample B and 20 ml of Tween 20 and stir them magnetically at 1000 r / min for 1 hour at room temperature. During the stirring process, slowly add 10 ml of Coptis chinensis root sample A drop by drop until the system changes from turbid to clear. Label it as mixed sample E.

[0103] 6. Preparation of mixed sample F

[0104] Take 10 ml of Coptis chinensis root sample B and 20 ml of Tween 20 and stir them magnetically at 1000 r / min for 1 hour at room temperature. During the stirring process, slowly add 10 ml of Scutellaria baicalensis root sample A drop by drop until the system changes from turbid to clear. Label it as mixed sample F.

[0105] 3. Antibacterial test

[0106] 1. Antibacterial test of samples containing Artemisia annua leaves

[0107] 1.1 Antibacterial test on Candida albicans

[0108] Candida albicans was evenly spread on TPY solid culture medium, and then the sterilized Oxford cup was placed on the test plate and gently pressed to make it contact with the culture dish without gaps. 100 μl of Qingsong leaf sample A, 100 μl of Qingsong leaf sample B, 200 μl of Qingsong leaf sample C, and 100 μl of Qingsong leaf sample D were injected into the Oxford cup respectively, and the size of the inhibition zone was measured after culturing at 37°C for 16 hours. The test results are shown in Table 1.

[0109] 1.2 Antibacterial test on Staphylococcus aureus

[0110] Staphylococcus aureus was evenly applied to TPY solid culture medium, and then the sterilized Oxford cup was placed in the test plate and gently pressed to make it contact with the culture dish without gaps. 100 μl of Qingsong leaf sample A, 100 μl of Qingsong leaf sample B, 200 μl of Qingsong leaf sample C, and 100 μl of Qingsong leaf sample D were injected into the Oxford cup respectively, and the size of the inhibition zone was measured after culturing at 37°C for 16 hours. The test results are shown in Table 2.

[0111] Table 1 Results of antibacterial test of Artemisia annua leaf samples on Candida albicans

[0112] Treatment Group Inhibition zone (mm) Qing Song Leaf Sample A 7.3 Qing Song leaf sample B 6.9 Green Song Leaf Sample C 10.7 Green Song Leaf Sample D 6.2

[0113] As shown in Table 1, Artemisia annua leaf sample C has the best inhibitory effect on Candida albicans, with an inhibition zone of 10.7 mm. Sample D, which is a physical mixture of Artemisia annua leaf samples A and B, exhibits a certain antagonistic effect and has the worst inhibitory effect.

[0114] Table 2 Results of antibacterial test of Artemisia annua leaf samples on Staphylococcus aureus

[0115] Treatment Group Inhibition zone (mm) Qing Song Leaf Sample A 9.1 Qing Song leaf sample B 7.7 Green Song Leaf Sample C 18.2 Green Song Leaf Sample D 7.5

[0116] As shown in Table 2, the antibacterial effect of the Artemisia annua leaf sample on Staphylococcus aureus is better than that on Candida albicans. The inhibition zone of Artemisia annua leaf sample C on Staphylococcus aureus reaches 18.2 mm, which is 9.1 mm higher than the inhibition zone of Artemisia annua leaf sample A alone.

[0117] 2. Antibacterial test of samples containing Magnolia officinalis bark

[0118] 2.1 Inhibition test on Candida albicans

[0119] Candida Albicans was evenly spread on TPY solid culture medium, and then the sterilized Oxford cup was placed on the test plate and gently pressed to make it contact with the culture dish without gaps. 100μl of Magnolia officinalis bark sample A, 100μl of Magnolia officinalis bark sample B, 200μl of Magnolia officinalis bark sample C, and 100μl of Magnolia officinalis bark sample D were injected into the Oxford cup respectively, and the size of the inhibition zone was measured after culturing at 37℃ for 16 hours. The test results are shown in Table 3.

[0120] 2.2 Inhibition test on Staphylococcus aureus

[0121] Staphylococcus aureus was evenly spread on TPY solid culture medium, and then the sterilized Oxford cup was placed on the test plate and gently pressed to make it contact with the culture dish without gaps. 100μl of Magnolia bark sample A, 100μl of Magnolia bark sample B, 200μl of Magnolia bark sample C, and 100μl of Magnolia bark sample D were injected into the Oxford cup respectively, and the size of the inhibition zone was measured after culturing at 37℃ for 16 hours. The test results are shown in Table 4.

[0122] Table 3 Magnolia officinalis bark samples against Candida albicans antibacterial test results

[0123] Treatment Group Inhibition zone (mm) Magnolia officinalis bark sample A 7.7 Magnolia officinalis bark sample B 9.2 Magnolia officinalis bark sample C 14.4 Magnolia officinalis bark sample D 7.1

[0124] Table 4 Magnolia bark samples inhibit Staphylococcus aureus test results

[0125] Treatment Group Inhibition zone (mm) Magnolia officinalis bark sample A 8.3 Magnolia officinalis bark sample B 10.3 Magnolia officinalis bark sample C 16.7 Magnolia officinalis bark sample D 8.1

[0126] 3. Antibacterial test of samples containing myrtle

[0127] 3.1 Inhibition test on Candida albicans

[0128] Candida Albicans was evenly spread on TPY solid culture medium, and then the sterilized Oxford cup was placed on the test plate and gently pressed to make it contact with the culture dish without gaps. 100μl of myrtle fruit sample A, 100μl of myrtle fruit sample B, 200μl of myrtle fruit sample C, 100μl of myrtle fruit sample D, 200μl of mixed sample A, and 200μl of mixed sample B were injected into the Oxford cup respectively, and the size of the inhibition zone was measured after culturing at 37℃ for 16 hours. The test results are shown in Table 5.

[0129] 3.2 Inhibition test on Staphylococcus aureus

[0130] Staphylococcus aureus was evenly spread on TPY solid culture medium, and then the sterilized Oxford cup was placed on the test plate and gently pressed to make it contact with the culture dish without gaps. 100 μl of myrtle fruit sample A, 100 μl of myrtle fruit sample B, 200 μl of myrtle fruit sample C, 100 μl of myrtle fruit sample D, 200 μl of mixed sample A, and 200 μl of mixed sample B were injected into the Oxford cup respectively, and the size of the inhibition zone was measured after culturing at 37°C for 16 hours. The test results are shown in Table 6.

[0131] It can be seen from Table 5 that the mixed sample A (myrtle sample B + Artemisia annua leaf sample A) has the best inhibitory effect on Candida albicans, and the mixed sample B (myrtle sample A + Artemisia annua leaf sample B) has a general inhibitory effect, and the inhibition zone after mixing is not as good as that of Artemisia annua leaf sample B.

[0132] It can be seen from Table 6 that the mixed sample A (myrtle sample B + Artemisia annua leaf sample A) has the best inhibitory effect on Staphylococcus aureus, and the mixed sample B (myrtle sample A + Artemisia annua leaf sample B) has a general inhibitory effect, and the inhibition zone after mixing is equivalent to that of the myrtle sample A.

[0133] Table 5 Inhibition test of Candida albicans containing samples of Myrtle

[0134] Treatment Group Inhibition zone (mm) Myrtle Fruit Sample A 6.3 Myrtle Fruit Sample B 8.9 Myrtle Fruit Sample C 11.7 Myrtle fruit sample D 6.1 Mixed Sample A 15.4 Mixed sample B 6.8

[0135] As can be seen from Table 5, the inhibition zone of myrtle sample B against Candida albicans is 8.9 mm, the inhibition zone of Artemisia annua leaf sample A against Candida albicans is 7.3 mm, and the inhibition zone after the two are mixed is 15.4 mm. The inhibition zone of myrtle sample C after the myrtle sample A and myrtle B sample are mixed is 11.7 mm, indicating that the combination of myrtle sample B and Artemisia annua leaf sample A shows a synergistic effect.

[0136] Table 6 Inhibition test of Staphylococcus aureus by samples containing Myrtle

[0137] Treatment Group Inhibition zone (mm) Myrtle Fruit Sample A 9.4 Myrtle Fruit Sample B 10.6 Myrtle Fruit Sample C 15.5 Myrtle fruit sample D 8.9 Mixed Sample A 18.8 Mixed sample B 9.8

[0138] It can be seen from Table 6 that the mixing of the components in Table 5 exhibits the same effect.

[0139] 4. Inhibition test of samples containing Sophora flavescens roots

[0140] 4.1 Inhibition test on Candida albicans

[0141] Candida Albicans was evenly spread on TPY solid culture medium, and then the sterilized Oxford cup was placed on the test plate and gently pressed to make it contact with the culture dish without gaps. 100μl of Sophora flavescens root sample A, 100μl of Sophora flavescens root sample B, 200μl of Sophora flavescens root sample C, 100μl of Sophora flavescens root sample D, 200μl of mixed sample C, and 200μl of mixed sample D were injected into the Oxford cup respectively, and the size of the inhibition zone was measured after culturing at 37℃ for 16 hours. The test results are shown in Table 6.

[0142] 4.2 Inhibition test on Staphylococcus aureus

[0143] Staphylococcus aureus was evenly spread on TPY solid culture medium, and then the sterilized Oxford cup was placed on the test plate and gently pressed to make it contact with the culture dish without gaps. 100μl of Sophora flavescens root sample A, 100μl of Sophora flavescens root sample B, 200μl of Sophora flavescens root sample C, 100μl of Sophora flavescens root sample D, 200μl of mixed sample A, and 200μl of mixed sample B were injected into the Oxford cup respectively, and the size of the inhibition zone was measured after culturing at 37℃ for 16 hours. The test results are shown in Table 7.

[0144] Table 6 Inhibition test of Candida albicans containing samples of Sophora flavescens root

[0145] Treatment Group Inhibition zone (mm) Sophora flavescens root sample A 9.8 Sophora flavescens root sample B 14.9 Sophora flavescens root sample C 21.2 Sophora flavescens root sample D 9.1 Mixed Sample C 22.3 Mixed Sample D 9.5

[0146] It can be seen from Table 6 that the inhibition zone of Sophora flavescens root sample A against Candida albicans is 9.8mm, and the inhibition zone of Sophora flavescens root sample B against Candida albicans is 14.9mm. As shown in Table 4, the inhibition zone of Magnolia officinalis bark sample A against Candida albicans is 7.7mm, and the inhibition zone of Magnolia officinalis bark B against Candida albicans is 9.2mm. The inhibition zone after mixing Sophora flavescens root B with Magnolia officinalis bark A is 22.3mm, which is equivalent to the antibacterial effect of Sophora flavescens root sample C.

[0147] Table 7 Inhibition test of Staphylococcus aureus by samples containing Sophora flavescens root

[0148] Treatment Group Inhibition zone (mm) Sophora flavescens root sample A 10.7 Sophora flavescens root sample B 17.5 Sophora flavescens root sample C 23.4 Sophora flavescens root sample D 10.1 Mixed Sample C 23.8 Mixed Sample D 12.4

[0149] As shown in Table 6 and Table 7, the inhibitory effect of mixed sample C (i.e., Radix Sophorae Flavescentis Sample B+Magnolia Officinalis Bark Sample A) on Candida albicans and Staphylococcus aureus is comparable to that of Radix Sophorae Flavescentis Sample C, but is significantly better than other samples. In the Candida albicans test, the inhibition zone of Radix Sophorae Flavescentis Sample B to Candida albicans is 14.9mm, the inhibition zone of Magnolia Officinalis Sample A to Candida albicans is 7.7mm, and the inhibition zone of mixed sample C after the two are mixed is 22.3mm. The inhibition zone of Radix Sophorae Flavescentis Sample A to Candida albicans is 9.8mm, the inhibition zone of Magnolia Officinalis Sample B to Candida albicans is 9.2mm, and the inhibition zone of mixed sample D after the two are mixed is only 9.5mm. The above test results show that the inhibitory effect of specific Radix Sophorae Flavescentis Sample and specific Magnolia Officinalis Sample on Candida albicans may have antagonism.

[0150] 5. Inhibition test of samples containing honeysuckle

[0151] 5.1 Inhibition test on Candida albicans

[0152] Candida Albicans was evenly spread on TPY solid culture medium, and then the sterilized Oxford cup was placed on the test plate and gently pressed to make it contact with the culture dish without gaps. 100μl of honeysuckle sample A, 100μl of honeysuckle sample B, 200μl of honeysuckle sample C and 100μl of honeysuckle sample D were injected into the Oxford cup respectively, and the size of the inhibition zone was measured after culturing at 37℃ for 16 hours. The test results are shown in Table 8.

[0153] 5.2 Inhibition test on Staphylococcus aureus

[0154] Staphylococcus aureus was evenly spread on TPY solid culture medium, and then the sterilized Oxford cup was placed on the test plate and gently pressed to make it contact with the culture dish without gaps. 100 μl of honeysuckle sample A, 100 μl of honeysuckle sample B, 200 μl of honeysuckle sample C and 100 μl of honeysuckle sample D were injected into the Oxford cup respectively, and the size of the inhibition zone was measured after culturing at 37°C for 16 hours. The test results are shown in Table 9.

[0155] Table 8 Inhibition test of honeysuckle samples on Candida albicans

[0156] Treatment Group Inhibition zone (mm) Honeysuckle Sample A 7.4 Honeysuckle Sample B 7.9 Honeysuckle Sample C 10.2 Honeysuckle Sample D 7.1

[0157] Table 9 Inhibition test of samples containing honeysuckle on Staphylococcus aureus

[0158]

[0159]

[0160] 6. Inhibition test of samples containing Coptis chinensis root

[0161] 6.1 Inhibition test on Candida albicans

[0162] Candida Albicans was evenly spread on TPY solid culture medium, and then the sterilized Oxford cup was placed on the test plate and gently pressed to make it contact with the culture dish without gaps. 100μl of Coptis chinensis root sample A, 100μl of Coptis chinensis root sample B, 200μl of Coptis chinensis root sample C and 100μl of Coptis chinensis root sample D were injected into the Oxford cup respectively, and the size of the inhibition zone was measured after culturing at 37℃ for 16 hours. The test results are shown in Table 10.

[0163] 6.2 Inhibition test on Staphylococcus aureus

[0164] Staphylococcus aureus was evenly applied to TPY solid culture medium, and then a sterilized Oxford cup was placed in the test plate and gently pressed to make it contact with the culture dish without gaps. 100 μl of Coptis chinensis root sample A, 100 μl of Coptis chinensis root sample B, 200 μl of Coptis chinensis root sample C and 100 μl of Coptis chinensis root sample D were injected into the Oxford cup respectively, and the size of the inhibition zone was measured after culturing at 37°C for 16 hours. The test results are shown in Table 11.

[0165] Table 10 Inhibition test of samples containing Coptis chinensis root on Candida albicans

[0166] Treatment Group Inhibition zone (mm) Coptis chinensis root sample A 9.7 Coptis chinensis root sample B 11.2 Coptis chinensis root sample C 14.7 Coptis chinensis root sample D 9.1

[0167] Table 11 Inhibition test of samples containing Coptis chinensis root on Staphylococcus aureus

[0168]

[0169]

[0170] 7. Inhibition test of samples containing Scutellaria baicalensis root

[0171] 7.1 Inhibition test on Candida albicans

[0172] Candida Albicans was evenly spread on TPY solid culture medium, and then the sterilized Oxford cup was placed on the test plate and gently pressed to make it contact with the culture dish without gaps. 100μl of Scutellaria root sample A, 100μl of Scutellaria root sample B, 200μl of Scutellaria root sample C, 100μl of Scutellaria root sample D, 200μl of mixed sample E and 200μl of mixed sample F were injected into the Oxford cup respectively, and the size of the inhibition zone was measured after culturing at 37℃ for 16 hours. The test results are shown in Table 12.

[0173] Table 12 Inhibition test of samples containing Scutellaria baicalensis root on Candida albicans

[0174] Treatment Group Inhibition zone (mm) Scutellaria baicalensis root sample A 9.7 Scutellaria baicalensis root sample B 11.2 Scutellaria baicalensis root sample C 14.7 Scutellaria baicalensis root sample D 9.1 Mixed Sample E 18.9 Mixed Sample F 15.7

[0175] It can be seen from Table 12 that the inhibitory activity of mixed sample E (i.e., Scutellaria root sample B and Coptis root sample A) against Candida albicans is better than that of mixed sample F (i.e., Coptis root sample B + Scutellaria root sample A), while the inhibitory activity of mixed sample F is slightly better than that of Scutellaria root sample C.

[0176] Table 13 Inhibition test of samples containing Scutellaria baicalensis root on Staphylococcus aureus

[0177]

[0178]

[0179] As shown in Table 13, the inhibitory activity of mixed sample E against Staphylococcus aureus is worse than that of Scutellaria baicalensis root sample C, and the inhibitory activity of mixed sample F is better than that of mixed sample E.

[0180] The above embodiments are preferred implementation schemes of the present invention. In addition, the present invention may also be implemented in other ways. Any obvious replacement without departing from the concept of the present invention is within the protection scope of the present invention.

Claims

1. A method for preparing a plant antibacterial spray composition, characterized in that: The steps include: S1 Preparation of Sample A The plant tissues were sorted, washed, and drained, and then placed in an oven for high-temperature sterilization, and then dried to constant weight. The dried plant tissues were crushed into plant tissue powder using a high-speed tissue crusher. After sieving, the plant tissue powder was weighed and extracted in water. After high-speed centrifugation, the precipitate was discarded, and the supernatant was filtered through a filter for later use, thereby obtaining sample A; S2 Preparation of Sample B The plant tissues are sorted, washed, and drained, and then placed in an oven for high-temperature sterilization, and then dried to constant weight. The dried plant tissues are crushed into plant tissue powder using a high-speed tissue crusher, and the plant tissue powder is weighed after sieving and extracted with ethanol reflux. The filtrate is concentrated under reduced pressure to an extract, and then dissolved in an organic solvent, filtered through a filter, and set aside to obtain sample B; S3 Preparation of Sample C Take 10 mL of sample B and emulsifier and stir magnetically at room temperature at a speed of 1000 r / min for 1 h. During the stirring process, add 10 mL of sample A drop by drop. When the system changes from turbid to clear, sample C is obtained. The high temperature in steps S1 and S2 is 80-150° C., the fixing time is 5-30 min, the weight ratio of plant tissue powder to water in step S1 is 1:5, the organic solvent in step S2 includes glycerol, propylene glycol, isopropanol, ethanol, propionic acid, and the ratio of plant tissue powder to organic solvent is 1:5; The plant tissue in step S1 is Artemisia annua leaf, and the plant tissue in step S2 is Myrtle fruit.

2. The method for preparing the plant antibacterial spray composition according to claim 1, characterized in that: The temperature of water in step S1 is 90° C. and the extraction time is 3 h; the ethanol reflux extraction time in step S2 is 3 h.

3. A plant antibacterial spray composition prepared according to the method for preparing a plant antibacterial spray composition according to claim 1 or 2.

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