A feed additive for effectively improving poultry feed conversion efficiency and its application

Abstract

The present invention relates to the technical field of feed additives, and provides a feed additive and application for effectively improving poultry feed conversion efficiency, wherein the feed additive comprises the following components in parts by weight: 5-15 parts of curcumin, 5-18 parts of resveratrol, 8-15 parts of ligustrazine, 15-25 parts of lauric acid, 5-15 parts of dihydromyricetin, 8-18 parts of quercetin, 2-10 parts of pterostilbene, 6-18 parts of icariin, 1-8 parts of arginine, 2-7 parts of betaine, and 2-8 parts of mogroside V. The feed additive is added to poultry feed at a ratio of 500-1000 mg / kg. Most of the raw material components in the feed additive of the present invention are extracted from natural plants, belong to medicinal and edible homologous components, do not have food safety problems, and can improve the mitochondrial metabolic function of poultry after being used in combination with complete feed, significantly reduce the feed-to-weight ratio, and improve the utilization efficiency of feed.

Description

Technical Field

[0001] The present invention relates to the technical field of feed additives, and in particular to a feed additive for effectively improving poultry feed conversion efficiency and application thereof. Background Art

[0002] In recent years, affected by various factors, the price of poultry feed raw materials has risen sharply, and the feed utilization efficiency directly affects the overall economic benefits of broiler and duck farming. Therefore, improving feed utilization efficiency is one of the important means to improve the quality and efficiency of the poultry feed industry. A large number of studies have confirmed that both genetic and feed factors affect poultry feed utilization efficiency. Although genetic improvement methods have improved poultry feed utilization efficiency to a certain extent, there are still great differences in poultry feed utilization efficiency between different strains of the same breed, different individuals of the same strain, and even full siblings.

[0003] Mitochondria produce a large amount of ATP and play an important role in heat production, cell apoptosis and cellular calcium homeostasis. The quality and function of mitochondria directly affect the efficiency of cellular energy supply, which in turn affects the efficiency of nutrient utilization in the body. Among them, mitochondrial structure and function are regulated by mitochondrial biogenesis. For example, when mitochondrial biogenesis and membrane potential are damaged, the efficiency of cellular oxidative phosphorylation and ATP synthesis will decrease, which will eventually lead to a decrease in the efficiency of animal feed utilization.

[0004] Dietary nutritional levels (energy or crude protein, etc.) or functional additives (such as bioactive substances) can improve poultry mitochondrial function, increase feed compensation and feed efficiency. Therefore, the production potential of poultry feed utilization should be fully tapped through feed nutrition means, so as to improve feed utilization efficiency and achieve green and efficient development of the poultry industry through targeted regulation of mitochondrial function through nutrition. Therefore, it is of great significance to develop a safe and effective feed additive to regulate poultry feed utilization efficiency. Summary of the invention

[0005] The purpose of the present invention is to provide a feed additive and application for effectively improving poultry feed conversion efficiency. The feed additive can promote mitochondrial function after being used in combination with feed, thereby improving feed conversion efficiency.

[0006] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0007] The invention provides a feed additive for effectively improving poultry feed conversion efficiency. The feed additive comprises the following components in parts by weight: 5-15 parts of curcumin, 5-18 parts of resveratrol, 8-15 parts of ligustrazine, 15-25 parts of lauric acid, 5-15 parts of dihydromyricetin, 8-18 parts of quercetin, 2-10 parts of pterostilbene, 6-18 parts of icariin, 1-8 parts of arginine, 2-7 parts of betaine and 2-8 parts of mogroside V.

[0008] Furthermore, the feed additive includes the following components in parts by weight: 8-12 parts of curcumin, 6-12 parts of resveratrol, 9-13 parts of ligustrazine, 18-23 parts of lauric acid, 8-12 parts of dihydromyricetin, 9-15 parts of quercetin, 3-7 parts of pterostilbene, 8-15 parts of icariin, 3-6 parts of arginine, 3-6 parts of betaine, and 4-6 parts of mogroside V.

[0009] Furthermore, the feed additive includes the following components in parts by weight: 10 parts of curcumin, 10 parts of resveratrol, 10 parts of ligustrazine, 20 parts of lauric acid, 10 parts of dihydromyricetin, 10 parts of quercetin, 5 parts of pterostilbene, 10 parts of icariin, 5 parts of arginine, 5 parts of betaine, and 5 parts of mogroside V.

[0010] The present invention also provides the use of the feed additive in improving poultry feed conversion efficiency, wherein the feed additive is added in an amount of 500 to 1000 mg / kg in the feed.

[0011] Compared with the prior art, the present invention has the following beneficial effects:

[0012] The raw material components of the feed additive of the present invention are mostly extracted from traditional Chinese medicine, which are natural plant-derived components, not antibiotics, and will not cause bacteria to develop drug resistance after use, nor will there be any food safety issues. The various active ingredients of traditional Chinese medicine in the feed additive of the present invention interact with each other and play a pharmacological role together, which can significantly improve the feed conversion efficiency of poultry. After feeding the feed to which the feed additive of the present invention is added, the function of mitochondria can be regulated by nutrition, the feed conversion efficiency can be promoted, nutrition can be increased, immunity can be enhanced, and the feed-to-weight ratio can be reduced. In addition, the raw material components in the feed additive are widely available and easy to obtain, so it is a safe and efficient feed additive. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 The effects of adding the feed additive of Example 1 to the diet and t-BHP injection on the expression of mitochondrial fusion protein-related genes and the PGC-1α signaling pathway in Cherry Valley ducks aged 28-42 days. DETAILED DESCRIPTION

[0014] The invention provides a feed additive for effectively improving poultry feed conversion efficiency. The feed additive comprises the following components in parts by weight: 5-15 parts of curcumin, 5-18 parts of resveratrol, 8-15 parts of ligustrazine, 15-25 parts of lauric acid, 5-15 parts of dihydromyricetin, 8-18 parts of quercetin, 2-10 parts of pterostilbene, 6-18 parts of icariin, 1-8 parts of arginine, 2-7 parts of betaine and 2-8 parts of mogroside V.

[0015] In the present invention, the feed additive that effectively improves poultry feed conversion efficiency includes 5 to 15 parts of curcumin, preferably 8 to 12 parts, and more preferably 9 to 11 parts, by weight.

[0016] In the present invention, the feed additive that effectively improves poultry feed conversion efficiency includes 5 to 18 parts of resveratrol, preferably 6 to 12 parts, and more preferably 8 to 10 parts by weight.

[0017] In the present invention, the feed additive that effectively improves poultry feed conversion efficiency includes 8 to 15 parts of ligustrazine, preferably 9 to 13 parts, and more preferably 10 to 12 parts, by weight.

[0018] In the present invention, the feed additive that effectively improves poultry feed conversion efficiency includes 15 to 25 parts of lauric acid, preferably 18 to 23 parts, and more preferably 19 to 21 parts, by weight.

[0019] In the present invention, the feed additive that effectively improves poultry feed conversion efficiency includes 5 to 15 parts of dihydromyricetin, preferably 8 to 12 parts, and more preferably 9 to 11 parts, by weight.

[0020] In the present invention, the feed additive that effectively improves poultry feed conversion efficiency includes 8 to 18 parts of quercetin, preferably 9 to 15 parts, and more preferably 10 to 13 parts by weight.

[0021] In the present invention, the feed additive that effectively improves poultry feed conversion efficiency includes 2 to 10 parts of pterostilbene, preferably 3 to 7 parts, and more preferably 4 to 6 parts by weight.

[0022] In the present invention, the feed additive that effectively improves poultry feed conversion efficiency includes 6 to 18 parts of icariin, preferably 8 to 15 parts, and more preferably 10 to 12 parts, by weight.

[0023] In the present invention, the feed additive that effectively improves poultry feed conversion efficiency includes 1 to 8 parts of arginine, preferably 3 to 6 parts, and more preferably 4 to 5 parts, by weight.

[0024] In the present invention, the feed additive that effectively improves poultry feed conversion efficiency includes 2 to 7 parts of betaine, preferably 3 to 6 parts, and more preferably 4 to 5 parts by weight.

[0025] In the present invention, the feed additive that effectively improves poultry feed conversion efficiency includes 2 to 8 parts of mogroside V, preferably 3 to 6 parts, and more preferably 4 to 5 parts, by weight.

[0026] In the present invention, the curcumin, resveratrol, ligustrazine, lauric acid, dihydromyricetin, quercetin, pterostilbene, icariin, arginine, betaine, and mogroside V are all commercially available.

[0027] In the present invention, the preparation method of the feed additive for effectively improving poultry feed conversion efficiency is: the feed additive is obtained after the raw materials are uniformly mixed according to weight portions.

[0028] The present invention also provides the use of the feed additive in improving poultry feed conversion efficiency, wherein the feed additive is added in an amount of 500 to 1000 mg / kg, preferably 600 to 900 mg / kg, and further preferably 700 to 800 mg / kg.

[0029] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0030] Example 1

[0031] This embodiment provides a feed additive for effectively improving poultry feed conversion efficiency, comprising the following components: 100 g of curcumin, 100 g of resveratrol, 100 g of ligustrazine, 200 g of lauric acid, 100 g of dihydromyricetin, 100 g of quercetin, 50 g of pterostilbene, 100 g of icariin, 50 g of arginine, 50 g of betaine, and 50 g of mogroside V. The raw materials are mixed according to their weight ratio to obtain the feed additive.

[0032] Example 2

[0033] This embodiment provides a feed additive for effectively improving poultry feed conversion efficiency, comprising the following components: 50 g of curcumin, 180 g of resveratrol, 150 g of ligustrazine, 150 g of lauric acid, 80 g of dihydromyricetin, 150 g of quercetin, 60 g of pterostilbene, 150 g of icariin, 40 g of arginine, 70 g of betaine, and 80 g of mogroside V. The feed additive is obtained by mixing the raw materials according to their weight ratio.

[0034] Example 3

[0035] This embodiment provides a feed additive for effectively improving poultry feed conversion efficiency, comprising the following components: 120 g of curcumin, 80 g of resveratrol, 120 g of ligustrazine, 240 g of lauric acid, 50 g of dihydromyricetin, 80 g of quercetin, 100 g of pterostilbene, 80 g of icariin, 60 g of arginine, 30 g of betaine, and 20 g of mogroside V. The raw materials are mixed according to their weight ratio to obtain the feed additive.

[0036] Experimental Example 1

[0037] 480 one-day-old yellow-feathered broilers were selected and randomly divided into 4 groups, with 6 replicates in each group and 20 chickens (all males) in each replicate. There was no significant difference in body weight between the replicates (P>0.05). The control group was fed a basal diet (CON group), and the added group added the feed additive of Example 1 to the basal diet, and the addition amount of the feed additive was 700 mg / kg. The test period was 56 days, of which 1 to 21 days old was the early stage of the test, 22 to 42 days old was the middle stage of the test, and 43 to 56 days old was the late stage of the test. The feed formula and nutritional composition during the test period are shown in Table 1. The growth of each group of yellow-feathered broilers after the end of the test period is shown in Table 2, and the effect of feed additives on the mitochondrial function of each group of yellow-feathered broilers is shown in Table 3.

[0038] Table 1 Feed formula and nutritional composition

[0039]

[0040]

[0041] Table 2 Growth status of yellow-feathered broiler chickens

[0042]

[0043]

[0044] As can be seen from the above table, compared with the CON group, the supplement group significantly increased the body weight and average daily weight gain of yellow-feathered broilers at each stage (P<0.05), and the supplement group significantly reduced the feed-to-weight ratio of yellow-feathered broilers at 1-21 days of age, 1-42 days of age, and 21-42 days of age (P<0.05), but had no significant effect on the feed-to-weight ratio in the later stage of 1-56 days of age (P<0.05).

[0045] Table 3 Effects of feed additives on mitochondrial function in yellow-feathered broilers

[0046]

[0047] As can be seen from the above table, compared with the CON group, the supplementation group significantly increased the contents of ATP, ADP, complex II and complex V in yellow-feathered broilers (P<0.05).

[0048] Experimental Example 2

[0049] This experimental example explored the effect of the feed additive of the present invention on the mitochondrial function of Cherry Valley ducks aged 28-42 days.

[0050] 1. Experimental Methods

[0051] A single-factor completely randomized experimental design was used to randomly divide 72 28-day-old meat ducks of the same strain into two groups. The control group was fed with a basic diet, and the supplement group was fed with a basic diet + the feed additive of Example 1, the addition amount of the feed additive was 700 mg / kg, each treatment group had 6 replicates, each replicate had 6 ducks, and the test period was 14 days. The feed formula and nutritional composition during the test period are shown in Table 1 for feed and nutritional levels at 21-56 days of age.

[0052] 2. Experimental results

[0053] 2.1 Effects of dietary feed additives on the production performance of Cherry Valley ducks aged 28-42 days

[0054] The effects of adding feed additives to the diet on the production performance of Cherry Valley ducks aged 28-42 days are shown in Table 4. As can be seen from Table 4, compared with the control group, adding feed additives to the diet significantly increased the final weight, average daily weight gain and feed-to-meat ratio of Cherry Valley ducks (P<0.05).

[0055] Table 4 Effects of dietary feed additives on growth performance of Cherry Valley ducks aged 28-42 days

[0056]

[0057] 2.2 Effects of dietary feed additives on mitochondrial function in Cherry Valley ducks aged 28-42 days

[0058] The effects of adding feed additives to the diet on the mitochondrial function of Cherry Valley ducks aged 28-42 days are shown in Table 5. As can be seen from Table 5, compared with the control group, adding feed additives to the diet significantly increased the relative expression of ND-1, ATP-8, COX-Ⅲ mt copy number and the content of complexes I, III, V and ATP in Cherry Valley ducks.

[0059] Table 5 Effects of dietary feed additives on mitochondrial function in Cherry Valley ducks aged 28-42 days

[0060]

[0061] Experimental Example 3

[0062] This experimental example explored the effect of the feed additive of the present invention on the mitochondrial function of Cherry Valley ducks aged 28-42 days.

[0063] 1. Experimental Methods

[0064] A 2×2 two-factor completely randomized experimental design (see Table 6) was used to select 144 28-day-old meat ducks of the same strain and randomly divided into 4 treatment groups: 2 mitochondrial damage treatments (PBS injection group, t-BHP injection group induced mitochondrial damage group) × 2 diet treatment groups [basic diet group (diet 1) and basic diet + Example 1 feed additive treatment group (diet 2)], each treatment group 6 replicates, each replicate 6, the experimental period 14d. The feed formula and nutritional composition during the experimental period are shown in Table 1 for the feed and nutritional levels of 21-56 days of age.

[0065] Index determination: growth performance index, mtDNA copy number (qPCR).

[0066] Mitochondrial function: tissue ATP content (fluorescent probe method), respiratory chain enzyme complex activity (colorimetric method); qPCR technology is planned to be used to analyze the tissue PGC-1α and mitochondrial fusion protein mRNA expression levels.

[0067] Feed was stopped at 20:00 every day before the start and end of the experiment. The experimental ducks were weighed and recorded in duplicate at 08:00 the next day. The daily feed consumption and residual feed amount of the experimental ducks were recorded in duplicate during the feeding period, and the average daily feed intake (AFDI), average daily weight gain (ADG) and feed-to-weight ratio (F / G) of the experimental ducks during the feeding stage were calculated.

[0068] Weight gain of each experimental duck = (final weight of each repeated experimental duck and initial weight of each repeated experimental duck) / number of ducks

[0069] Feed intake of each experimental duck = total feed consumption per replicate

[0070] Average daily gain (ADG) = number of days of weight gain test for each experimental duck

[0071] Average daily feed intake (AFDI) = feed intake of each experimental duck for the number of experimental days

[0072] Feed-to-weight ratio (F / G) = average daily feed intake / average daily weight gain

[0073] Table 6 Experimental design

[0074]

[0075] 2. Experimental results

[0076] 2.1 Dietary Addition Example 1 Effects of feed additives and t-BHP injection on the production performance of Cherry Valley ducks aged 28-42 days

[0077] The effects of adding the feed additive of Example 1 to the diet and t-BHP injection on the production performance of Cherry Valley ducks aged 28-42 days are shown in Table 7. As can be seen from Table 7, the final weight, average daily weight gain and feed-to-meat ratio of Cherry Valley ducks were significantly reduced after t-BHP injection (P<0.05), and the final weight, average daily weight gain and feed-to-meat ratio of Cherry Valley ducks were significantly increased after adding the feed additive of Example 1 to the diet (P<0.05). There was no interactive effect between the addition of the feed additive of Example 1 and t-BHP injection on the final weight and average daily weight gain of Cherry Valley ducks.

[0078] Table 7 Effects of dietary feed additives and t-BHP injection on growth performance of Cherry Valley ducks aged 28-42 days

[0079]

[0080]

[0081] 2.2 Oxidative damage and pathological changes in the liver of Cherry Valley ducks aged 28-42 days induced by t-BHP injection

[0082] The oxidative damage and pathological changes in the liver of Cherry Valley ducks aged 28-42 days caused by injection of t-BHP are shown in Table 8. It can be seen from Table 8 that compared with injection of PBS, injection of t-BHP significantly increased the 8-OHdG and MDA contents of Cherry Valley ducks (P<0.05), and significantly reduced the level of antioxidant anions (P<0.05); compared with diet 1, diet 2 significantly increased the level of antioxidant anions in Cherry Valley ducks (P<0.05). During the experiment, the 8-OHdG and MDA contents of Cherry Valley ducks were significantly affected by the interaction effect of diet and injection (P<0.05).

[0083] Table 8 Oxidative damage and pathological changes in the liver of Cherry Valley ducks aged 28-42 days induced by t-BHP injection

[0084]

[0085] 2.3 Dietary Addition Example 1 Effects of feed additives and t-BHP injection on mitochondrial copy number in Cherry Valley ducks aged 28-42 days

[0086] The effects of feed additives added to diets in Example 1 and t-BHP injection on the mitochondrial copy number of Cherry Valley ducks aged 28-42 days are shown in Table 9. As shown in Table 9, compared with PBS injection, t-BHP injection significantly reduced the gene expression levels of ATP-6 and COX-Ⅲ (P<0.05), and compared with diet 1, diet 2 significantly increased the expression levels of ND-1, ND-2, ATP-6, ATP-8, and COX-Ⅲ (P<0.05).

[0087] Table 9 Effects of dietary additives and t-BHP injection on mitochondrial copy number in Cherry Valley ducks aged 28-42 days

[0088]

[0089] 2.4 Dietary Addition Example 1 Effects of Feed Additives and t-BHP Injection on the Respiratory Chain Complex of Cherry Valley Ducks Aged 28-42 Days

[0090] The effects of feed additives added to diets in Example 1 and t-BHP injection on the respiratory chain complexes of Cherry Valley ducks aged 28-42 days are shown in Table 10. As can be seen from Table 10, compared with injection of PBS, injection of t-BHP significantly reduced the content of complexes I, II, III, and IV (P<0.05), and compared with diet 1, diet 2 significantly increased the content of complexes I, II, III, and IV (P<0.05).

[0091] Table 10 Effects of dietary additives and t-BHP injection on respiratory chain complexes in Cherry Valley ducks aged 28-42 days

[0092]

[0093]

[0094] 2.5 Dietary Addition Example 1 Effects of feed additives and t-BHP injection on mitochondrial function of Cherry Valley ducks aged 28-42 days

[0095] The effects of feed additives added to diets in Example 1 and t-BHP injection on mitochondrial function of Cherry Valley ducks aged 28-42 days are shown in Table 11. As shown in Table 11, compared with PBS injection, t-BHP injection significantly reduced the contents of ATP, ADP and AMP (P<0.05), and compared with diet 1, diet 2 significantly increased the contents of ATP, ADP and AMP (P<0.05).

[0096] Table 11 Effects of dietary additives and t-BHP injection on mitochondrial function in Cherry Valley ducks aged 28-42 days

[0097]

[0098] 2.6 Dietary Addition Example 1 Effects of feed additives and t-BHP injection on the expression of mitochondrial fusion protein-related genes and PGC-1α signaling pathway in 28-42-day-old Cherry Valley ducks

[0099] Results Figure 1 ,Depend on Figure 1It can be seen that compared with the injection of PBS, the injection of t-BHP significantly reduced the expression levels of liver genes PGC-1α and MFN-1 (P<0.05); compared with diet 1, diet 2 significantly upregulated the expression of liver mitochondrial fusion protein-related genes MFN-1, MFN-2, and OPA1 (P<0.05).

[0100] It can be seen from the above experiments that adding the feed additive of the present invention to the diet can improve the mitochondrial function of poultry and further improve the feed conversion efficiency of poultry.

[0101] It can be seen from the above embodiments and experimental examples that the present invention provides a feed additive and application that effectively improves poultry feed conversion efficiency. The feed additive can promote mitochondrial function when used in combination with feed, thereby improving feed conversion efficiency.

[0102] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A feed additive for effectively improving the feed conversion efficiency of poultry, characterized in that: The invention is composed of the following components in parts by weight: 5-15 parts of curcumin, 5-18 parts of resveratrol, 8-15 parts of ligustrazine, 15-25 parts of lauric acid, 5-15 parts of dihydromyricetin, 8-18 parts of quercetin, 2-10 parts of pterostilbene, 6-18 parts of icariin, 1-8 parts of arginine, 2-7 parts of betaine and 2-8 parts of mogroside V.

2. The feed additive according to claim 1, characterized in that The invention is composed of the following components in parts by weight: 8-12 parts of curcumin, 6-12 parts of resveratrol, 9-13 parts of ligustrazine, 18-23 parts of lauric acid, 8-12 parts of dihydromyricetin, 9-15 parts of quercetin, 3-7 parts of pterostilbene, 8-15 parts of icariin, 3-6 parts of arginine, 3-6 parts of betaine and 4-6 parts of mogroside V.

3. The feed additive according to claim 1, characterized in that The invention is composed of the following components in parts by weight: 10 parts of curcumin, 10 parts of resveratrol, 10 parts of ligustrazine, 20 parts of lauric acid, 10 parts of dihydromyricetin, 10 parts of quercetin, 5 parts of pterostilbene, 10 parts of icariin, 5 parts of arginine, 5 parts of betaine and 5 parts of mogroside V.

4. Use of the feed additive according to any one of claims 1 to 3 in improving feed conversion efficiency of poultry, characterized in that: The feed additive is added in an amount of 500-1000 mg / kg in the feed.

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