A functional composition with multiple repairing effects and its preparation method and application

Abstract

The present invention relates to a functional composition with multiple repairing effects, a preparation method and an application thereof, wherein the functional composition comprises phytosterol rapeseed ester, madecassoside and ceramide liposome in a mass ratio of (0.1-1): (0.01-1): (0.1-3). Compared with the prior art, the present invention adopts madecassoside, phytosterol rapeseed ester and ceramide liposome, which have very good anti-inflammatory, repairing and penetration-promoting functional ingredients, and is supplemented with some conventional moisturizing and repairing ingredients such as panthenol, allantoin, tocopherol, sodium hyaluronate, avocado tree fat and saccharide isomers, to prepare a deep repair cream that can be absorbed through the skin, has anti-inflammatory and soothing effects, deep repair and quick onset.

Description

Technical Field

[0001] The invention belongs to the field of cosmetics, and in particular relates to a functional composition with multiple repairing effects, a cream, and a preparation method and application thereof. Background Art

[0002] The skin is the outermost organ of the human body and an important part of the body. It is thin and tough and covers the whole body. The skin can protect the body from external mechanical, physical, chemical stimulation and microbial invasion, and can also reflect and participate in the changes in the body's spirit and nutrition. Ordinary repair creams on the market often have a single function and little effect, and the effect is slow or has no effect. For example:

[0003] Some people, due to genetic factors or a history of dermatitis, will experience localized skin itching, which recurs frequently and is extremely uncomfortable. There are drugs on the market specifically for treating dermatitis, which have an immediate anti-itching effect, but they are not long-lasting and require repeated application, and are prone to drug dependence.

[0004] The effects of commercially available eczema creams are often unsatisfactory, slow to take effect, and prone to recurrence. There was once a very effective eczema cream on the market, but it was later revealed that it contained hormones and had drug resistance. Ordinary repair creams on the market have little effect on eczema, are slow to take effect, or have no effect.

[0005] The cracking cream that sells well on the market has Vaseline as its key ingredient. It mainly has a moisturizing and sealing effect. It has a moisturizing effect when applied, but once you stop applying it, the skin will still dry out and crack, and the problem of skin cracking has not been fundamentally repaired. There is a lack of creams on the market that have good transdermal absorption, deep repair, and quick effect. A repair and treatment cream that does not rely on hormone treatment, can be absorbed through the skin, deeply repairs, and has a quick effect, and can fundamentally solve these stubborn skin problems, is particularly necessary. Summary of the invention

[0006] The purpose of the present invention is to overcome the defects of the above-mentioned prior art and provide a functional composition, cream and its preparation method and application with multiple repairing effects.

[0007] The purpose of the present invention can be achieved by the following technical scheme: a functional composition with multiple repair effects, comprising phytosterol rapeseed oleate, madecassoside, and ceramide liposomes in a mass ratio of (0.1-1): (0.01-1): (0.1-3).

[0008] Furthermore, the mass ratio of the phytosterol canola oleate, madecassoside, and ceramide liposome is (0.1-1): (0.05-0.5): (0.2-1).

[0009] Madecassoside is one of the most important molecules in Centella asiatica and is an active ingredient with soothing effects. It effectively fights all the effects of inflammation, thus relieving the stress caused by sensitive skin. It has a good effect on skin desquamation and anti-redness.

[0010] Phytosterol rapeseed oil esters can improve the skin's contact feel, especially the β-sitosterol it contains has a high permeability to the skin, promotes sebum secretion, keeps the skin moist and soft, and can restore the softness of dry and hardened keratinized skin, and has a good effect on anti-inflammatory and repair.

[0011] Ceramide liposomes, with biomimetic ingredients and structures, use lecithin to encapsulate the physiological lipid components of human skin to obtain bilayer liposomes with extremely high skin affinity; high bioavailability: liposome particles are small, permeability is good, and they are easily used by skin active cells to build skin barriers; liposomes contain high levels of ceramide, which are especially suitable for dry skin and skin with damaged stratum corneum barriers. Ceramide alone is selective for oils and fats during use, the formula is relatively complex, and there is a risk of precipitation, so it is generally not recommended for direct use. The present invention uses ceramide encapsulated by liposomes (preferably ceramide 3) to form an active ingredient reservoir between the epidermis and the dermis after percutaneous absorption, and the active ingredients can be released sustainably to improve bioavailability, so as to achieve the purpose of long-term and high efficiency.

[0012] Furthermore, the phytosterol rapeseed oleate is first mixed and dissolved with cetostearyl alcohol, avocado butter, squalane and tocopherol before use.

[0013] Furthermore, the mass ratio of cetostearyl alcohol, phytosteryl canola oil ester, avocado butter, squalane and tocopherol is (0.5-3): (0.1-1): (0.5-3): (0.5-5): (0.1-0.5).

[0014] Furthermore, the madecassoside and ceramide liposome are mixed with sugar isomers and preservatives before use.

[0015] Furthermore, the mass ratio of madecassoside, ceramide liposome, carbohydrate isomers and preservative is (0.01-1): (0.1-3): (0.1-0.5): (0.6-1).

[0016] The present invention adopts madecassoside, phytosterol rapeseed ester, ceramide liposome, which have excellent anti-inflammatory, repairing and penetration-promoting effects, and is supplemented with some moisturizing and repairing ingredients such as panthenol, allantoin, tocopherol, sodium hyaluronate, avocado tree fat, and sugar isomers to prepare a deep repair cream that can be absorbed through the skin, has anti-inflammatory and soothing effects, deeply repairs, and takes effect quickly.

[0017] The present invention also provides a cosmetic containing the functional composition with multiple repairing effects, and the functional composition is used in the cosmetic.

[0018] Furthermore, the cosmetics include essence, facial mask and cream.

[0019] Furthermore, the cosmetic is a cream, including phase A, phase B, phase C, and phase D, each phase including the following raw materials in percentage by mass:

[0020] Phase A:

[0021]

[0022] Phase B:

[0023]

[0024] Phase C:

[0025] Neutralizer 0.1-0.3%;

[0026] Phase D:

[0027]

[0028] The present invention also provides a method for preparing the cream of the above-mentioned functional composition with multiple repairing effects, comprising the following steps:

[0029] 1) Add water to a water pot, add carbomer, disperse evenly, add the remaining ingredients of phase A, stir thoroughly, heat to 80-85° C., keep warm and stir for 30 minutes to obtain a phase A mixture;

[0030] 2) Add the raw materials of phase B into the oil phase pot, stir and heat at 80-85°C until completely dissolved to obtain a phase B mixture;

[0031] 3) The phase A mixture obtained in step 1) is pumped into an emulsifying pot, and the phase B mixture is pumped into the emulsifying pot under homogenous stirring, and homogenized for 3 to 10 minutes;

[0032] 4) Add phase C raw materials and stir evenly;

[0033] 5) When the temperature drops to 40-45°C, add the phase D raw materials into the emulsifying pot, stir evenly and then discharge the material to obtain the cream product.

[0034] Compared with the prior art, the present invention has the following beneficial effects: the present invention adopts madecassoside, phytosterol rapeseed ester, ceramide liposomes, which have excellent anti-inflammatory, repairing and penetration-promoting effects, and is supplemented with some moisturizing and repairing ingredients such as panthenol, allantoin, tocopherol, sodium hyaluronate, avocado tree fat, and sugar isomers to prepare a deep repair cream that can be absorbed through the skin, has anti-inflammatory and soothing effects, deeply repairs, and takes effect quickly. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] Figure 1 This is a comparison chart of mouse skin retention. DETAILED DESCRIPTION

[0036] The following is a detailed description of an embodiment of the present invention. This embodiment is implemented on the premise of the technical solution of the present invention, and a detailed implementation method and a specific operation process are given, but the protection scope of the present invention is not limited to the following embodiment.

[0037] The present invention relates to a combination of ingredients with multiple repairing effects and its application. The present invention adopts madecassoside, phytosterol rapeseed oleate, ceramide liposomes, which have excellent anti-inflammatory, repairing and penetration-promoting effects, supplemented with some moisturizing and repairing ingredients such as panthenol, allantoin, tocopherol, sodium hyaluronate, avocado tree fat, and sugar isomers, to prepare a deep repair cream that can be absorbed through the skin, has anti-inflammatory and soothing effects, deep repair, and rapid onset. Among them:

[0038] Madecassoside: Sensitive skin is more susceptible to inflammation. The epidermis becomes inflamed due to the release of inflammatory mediators. Redness and heat in the skin due to vasodilation indicate that actual inflammation has occurred. Due to excessive proliferation, the skin becomes thicker than usual, and the lack of internal tissue also changes the appearance of the skin. The aglycones in madecassoside ensure that the plant cells are protected from attacks by bacteria, fungi and yeast in the natural environment. The triterpenoid components are molecules with therapeutic properties, which have an effect on stretch marks, wrinkles or cellulite, moisturize and tighten the skin, and have anti-acne effects. The polyphenol components have antioxidant effects, as well as anti-inflammatory, UV / visible light absorption, and free radical resistance. The flavonoid components have anti-inflammatory and anti-allergic effects.

[0039] Phytosterol canola ester is a natural active substance found in a variety of fruits, vegetables, beans, nuts, cereals and other plant foods. Its structure is a steroidal compound with a 3-hydroxyl group, with cyclopentane perhydrophenanthrene as the main skeleton, accounting for the majority of tetracyclic diterpenoid compounds, among which the β-sitosterol content of phytosterol canola ester (a type of phytosterol) is 40-58%. β-sitosterol has a high permeability to the skin, keeps the skin moist and soft, and can restore the softness of dry and hardened keratin skin, prevent and treat skin sunburn, fight inflammation, repair, and even prevent and inhibit the formation of corns.

[0040] Ceramide liposomes, with biomimetic ingredients and structures, use lecithin to encapsulate the physiological lipid components of human skin to obtain bilayer liposomes with extremely high skin affinity; high bioavailability: the liposome particles are small and have good permeability, and are easily used by active skin cells to build a skin barrier; high content of ceramide, preferably ceramide 3, is especially suitable for dry skin and skin with damaged stratum corneum barrier.

[0041] The present invention prepares a moisturizing repair cream from the perspective of transdermal absorption, anti-inflammatory relief, deep repair, and multi-target combination. It has a quick and good repair effect on baby eczema, has no drug dependence, is available at any time, can fundamentally repair the skin barrier problem, treats both the symptoms and the root causes, and has a good therapeutic effect.

[0042] The various raw materials used in the present invention are all commercially available products in the field. For example:

[0043] Ceramide liposomes can be commercially available products, such as TAKASAGO;

[0044] The neutralizer is a commonly used neutralizer in the art, such as arginine, aminomethyl propanol, sodium hydroxide, potassium hydroxide, etc.

[0045] The preservatives are commonly used preservatives in this field, such as: p-hydroxyacetophenone, hexylene glycol, phenoxyethanol, ethylhexylglycerin, etc.

[0046] Examples 1-3, Comparative Examples 1-7

[0047] The formulations of the embodiments and comparative examples are shown in Table 1 below (the numerical units are in mass %):

[0048]

[0049]

[0050] The preparation method of the above cream comprises the following steps:

[0051] 1) Add water to a water pot, add carbomer, disperse evenly, add the remaining ingredients of phase A, stir thoroughly, heat to 82°C, keep warm and stir for 30 minutes, and keep warm for later use;

[0052] 2) Add the raw materials of phase B into the oil phase pot, stir and heat to 82°C until completely dissolved, and keep warm for later use;

[0053] 3) The raw material of phase A obtained in step 1) is pumped into an emulsifying pot, and the raw material of phase B is pumped into the emulsifying pot under homogenous stirring, and homogenized for 5 minutes;

[0054] 4) Add phase C raw materials and stir evenly;

[0055] 5) When the temperature drops to 42°C, add the raw materials of phase D into the emulsifying pot, stir evenly and then discharge the materials to obtain a cream with multiple functions.

[0056] In Comparative Example 8, compared with Example 2, phytosterol canola oleate was replaced with an equal amount of phytosterol oleate, and the rest were the same.

[0057] Comparative Example 9, compared with Example 2, ceramide liposomes were replaced with an equal amount of ceramide 3, and the rest were the same.

[0058] Effect verification test example

[0059] 1. Security Testing

[0060] Test samples: Examples 1-3 and Comparative Examples 1-9

[0061] Test subjects: 60 subjects were randomly divided into 12 groups, with 5 people in each group.

[0062] Test method: Use a third-party testing agency to test safety. Select an area not exceeding 50mm 2 , a suitable patch tester with a depth of about 1mm, add about 0.02-0.025ml (g) of the test sample into the patch tester by the closed patch test method. The patch tester is attached to the curved side of the subject's forearm, and the test substance is removed after 24 hours. The skin reaction is observed at 0.5h, 24h, and 48h after removal, and the results are recorded according to the skin reaction grading standards in the "Cosmetic Safety Technical Regulations 2015".

[0063] Table 4 Patch test results

[0064] Notes: Skin reaction grading standards;

[0065] Grade 0: negative reaction;

[0066] Grade 1: Suspicious reaction; only slight erythema;

[0067] Grade 2: weak positive reaction (erythema reaction); erythema, infiltration, edema, and papules may be present;

[0068] Grade 3: Strong positive reaction (herpes reaction); erythema, infiltration, edema, papules, herpes; the reaction may extend beyond the test area;

[0069] Grade 4: Very strong reaction (confluent herpes reaction); obvious erythema, severe infiltration, edema, confluent herpes, and the reaction exceeds the test area.

[0070] Table 2

[0071]

[0072]

[0073] The results showed that the products of Examples 1-3 and Comparative Examples 1-9 scored 0 at 30 minutes, 24 hours, and 48 hours, and no adverse skin reactions occurred, indicating that the cream products of the present invention are safe and non-irritating to the skin.

[0074] 2. Stability test

[0075] Test samples: Examples 1-3;

[0076] Test method: Internal control standard: observe the appearance color and pH for three months:

[0077] A: No change; B: Very slight change, basically unnoticeable without standard sample comparison; C: Slight change, noticeable even without standard sample comparison; D: Obvious change, noticeable to consumers; E: Severe change,

[0078] The standard sample is the product placed at 25℃;

[0079] Table 3 Stability test results (W stands for week, M stands for month, and cycle stands for -15°C to 50°C cycle)

[0080] time 50℃ 40℃ 45℃ 25℃ 5℃ -15℃ cycle illumination 1W A A A A A A A A 2W A A A A A A A A 3W A A A A A A A A 4W A A A A A A A A 2M B A A A A A B B 3M B A A A A A B B

[0081] It can be seen from Table 3 above that the color, viscosity and pH of the creams prepared in Examples 1-3 did not change significantly under high and low temperature conditions or under light exposure within three months, indicating that the eye cream of the present invention has good stability.

[0082] 3. Product effect evaluation

[0083] 1) In vitro permeability test

[0084] Take healthy SPF male mice, the number of a batch of purchases is 24, the mice are killed by neck breaking, the abdominal skin is cut, the fat layer is removed, the hair of the mouse epidermis is scraped, and it is washed with physiological saline and preserved uniformly. Randomly select mouse skin and ensure that the thickness of mouse skin is similar. After selection, it is fixed between the diffusion cell and the receiving cell, the dermis of the skin faces the receiving cell, the stratum corneum faces the diffusion cell, and the volume of the receiving cell is 15mL. The dermis of the skin is just in contact with the solvent in the receiving cell to prevent bubbles from being generated. The receiving solution is 50% ethanol aqueous solution, 1g sample (Example 1-3 and Comparative Example 1-9) is added to the supply cell, and the receiving cell is placed on the magnetic stirrer of the intelligent transdermal experiment instrument, and the water bath temperature is set to 37°C, the stirring speed is 350r / min, and the transdermal time is 12h. After the test, the skin was removed, and the surface of the mouse skin was wiped and rinsed with 50% ethanol aqueous solution, placed on filter paper and dried at room temperature, and then the mouse skin was moved to a small beaker, cut into pieces with scissors, added with 50% ethanol aqueous solution, ultrasonically extracted for 30 minutes, and soaked for 24 hours. The extract was filtered through a 0.22μm microporous filter membrane and then chromatographically analyzed to calculate the skin retention rate of different samples. The samples obtained in Examples 1-3 and Comparative Examples 1-9 were taken, and the above experiment was repeated to obtain the skin retention rate of different samples. The average value of the two tests of the same sample was taken and compared, as follows:

[0085] Skin retention Q(μg / cm 2 ), the calculation formula is Q = Vc / A, where A is the effective diffusion area, V is the total volume of the skin extract, and c is the mass concentration of the sample in the skin extract.

[0086] like Figure 1 As shown, it is a comparison chart of mouse skin retention of Examples 1-3 and Comparative Examples 1-9. It can be seen from the experimental results that Examples 1, 2, 3> Comparative Examples 8, 9> Comparative Examples 4, 5, 6> Comparative Examples 1, 2, 3 show strong skin retention capacity. Good skin retention capacity indicates that the sample absorption amount will be higher and the permeability is better. It can be seen that the skin retention capacity shown by Examples 1-3 containing three functional compositions of the present application at the same time is better than Comparative Examples 4-6 with only two functional components, and is better than Comparative Examples 1-3 with only one functional component, proving that a good synergistic effect is produced between the three functional components of the present invention. In addition, the skin retention capacity shown by Examples 1-3 is better than Comparative Examples 8-9, proving that the three functional components of the present invention are specific choices. Therefore, the functional composition of the specific components and dosage of the present invention has good skin permeability and absorbability.

[0087] 2) Repair efficacy analysis experiment (skin barrier repair for lactic acid stingers)

[0088] Research subjects: 120 healthy subjects were selected. Inclusion criteria: aged 25 to 40 years old, subjects had no visible skin diseases on their faces, and were all lactic acid stinging subjects during the first visit screening. During the test period, the subjects used the skin care products prepared in the embodiments and comparative examples on their faces, did not use other skin care products, and avoided excessive sun exposure.

[0089] Test process: The subjects sit quietly in a constant temperature and humidity room for 30 minutes, then use equal amounts of 10% lactic acid aqueous solution and distilled water on the left and right nasolabial grooves of the subjects, and ask the subjects about their skin symptoms after 5 minutes, and score them according to the four-point system: no tingling sensation is 0 points, mild tingling sensation is 1 point, moderate tingling sensation is 2 points, and severe tingling sensation is 3 points. The test of the same volunteer is completed by the same measurement personnel.

[0090] Skin repair test: 120 subjects were randomly divided into ten groups, and each group used the repairing and moisturizing creams obtained in Examples 1-3 and Comparative Examples 1-9 every morning and evening, and the lactic acid stinging test was repeated on the 0th day, 14th day and 28th day after using the products. The test results are shown in Table 4, and the results are averaged.

[0091] Table 4 Lactic acid stinging test results

[0092] Lactic acid sting value Day 0 14 days 28 days Example 1 3 1.8 1.5 Example 2 3 1.7 1.3 Example 3 3 1.6 1.3 Comparative Example 1 3 2.7 2.7 Comparative Example 2 3 2.6 2.5 Comparative Example 3 3 2.8 2.8 Comparative Example 4 3 2.0 1.9 Comparative Example 5 3 2.1 2.1 Comparative Example 6 3 2.1 2.0 Comparative Example 7 3 2.9 2.8 Comparative Example 8 3 1.9 1.7 Comparative Example 9 3 2.0 1.8

[0093] It can be seen from the results in Table 4 that the creams described in Examples 1-3 and Comparative Examples 1-9 of the present invention can effectively repair the skin barrier and reduce the lactic acid stinging value, while the cream obtained in Comparative Example 7 which does not contain a composition having both skin barrier repair function and moisturizing function does not have the above effect. The repair effect is ranked as Example 1, 2, 3 > Comparative Examples 8, 9 > Comparative Examples 4, 5, 6 > Comparative Examples 1, 2, 3, proving that the synergistic effect of the three main functional ingredients is the best, and has a better skin barrier repair effect than a simple combination of one or two ingredients.

[0094] 3) Cream effect experiment

[0095] Eczema: 30 volunteers diagnosed with eczema were recruited, aged 1-5 years old (with the consent of their guardians), and divided into three groups, 10 people in each group. The creams described in Examples 1-3 and Comparative Examples 1-9 were used on the problem areas for one week, and the changes in skin itching, erythema and papules before and after use were compared, measured by the volunteers' self-perception. The test results were expressed by comparing with the original values, and each parameter took the average value of the patients. The original value was the value before use, defined as 10, where the original value of the degree was 10, slightly reduced was defined as 9-7, reduced was 6-4, and significantly reduced was 3-0. The test results are shown in Table 4.

[0096] Table 4 Effect of using cream

[0097]

[0098] From the data in Table 4, it can be seen that the creams described in Examples 1-3 and Comparative Examples 1-9 are effective to a certain extent in the treatment of skin itching, erythema and papules, among which Examples 2 and 3 have the best effects, Example 1 has a slightly worse effect, Comparative Examples 4-6 are a little worse, Comparative Examples 1-3 are second, and Comparative Example 7 is third. This shows that using one of the main active ingredients can improve the efficacy, using two can have an additive effect, and the synergistic effect of the three main active ingredients is the best. After reaching the optimal effective amount, the effect is no longer additive.

Claims

1. A functional composition with multiple repair effects, characterized in that: It includes phytosterol canola oleate, madecassoside, and ceramide liposomes in a mass ratio of (0.1-1):(0.05-0.5):(0.2-1).

2. The functional composition with multiple repairing effects according to claim 1, characterized in that: Before use, the phytosterol rapeseed ester is first mixed and dissolved with cetostearyl alcohol, avocado butter, squalane and tocopherol.

3. The functional composition with multiple repairing effects according to claim 2, characterized in that: The mass ratio of cetostearyl alcohol, phytosteryl canola oil ester, avocado butter, squalane and tocopherol is (0.5-3): (0.1-1): (0.5-3): (0.5-5): (0.1-0.5).

4. The functional composition with multiple repairing effects according to claim 1, characterized in that: The madecassoside and ceramide liposome are mixed with sugar isomers and preservatives before use.

5. The functional composition with multiple repairing effects according to claim 4, characterized in that: The mass ratio of madecassoside, ceramide liposome, carbohydrate isomers and preservative is (0.01-1): (0.1-3): (0.1-0.5): (0.6-1).

6. A cosmetic containing the functional composition with multiple repairing effects as claimed in claim 1, characterized in that: The functional composition is used in cosmetics.

7. The cosmetic composition with multiple repairing effects according to claim 6, characterized in that: The cosmetics include essence, facial mask and cream.

8. The cosmetic composition with multiple repairing effects according to claim 7, characterized in that: The cosmetic is a cream, characterized in that it includes phase A, phase B, phase C, and phase D, and each phase includes the following raw materials in percentage by mass: Phase A: Water Residue Carbomer 0.1-0.3%; Glycerol 2-6%; Butanediol 2-6%; Disodium EDTA 0.02-0.1%; Sodium hyaluronate 0.01-0.5%; Allantoin 0.01-0.5%; Panthenol 0.1-5.0%; Hydrogenated lecithin 0.1-2.0%; Phase B: Cetostearyl alcohol 0.5-3.0%; Phytosterol canola oleate 0.1-1%; Avocado butter 0.5-3%; Squalane 0.5-5%; Tocopherol 0.1-0.5%; Phase C: Neutralizer 0.1-0.3%; Phase D: Madecassoside 0.01-1%; Ceramide liposome 0.1-3%; Sugar isomers 0.1-7%; Preservative 0.6-1%.

9. The method for preparing the cream of the functional composition with multiple repairing effects according to claim 8, characterized in that: The following steps are involved: 1) Add water to a water pot, add carbomer, disperse evenly, add the remaining ingredients of phase A, stir thoroughly, heat to 80-85°C, keep warm and stir for 30 minutes to obtain a phase A mixture; 2) Add the raw materials of phase B into the oil phase pot, stir and heat to 80-85℃ until completely dissolved to obtain a phase B mixture; 3) The phase A mixture obtained in step 1) is pumped into an emulsifying pot, and the phase B mixture is pumped into an emulsifying pot under homogenizing stirring, and homogenized for 3 to 10 minutes; 4) Add Phase C ingredients and stir evenly; 5) When the temperature drops to 40-45°C, add phase D raw materials into the emulsifying pot, stir evenly and then discharge the material to obtain the cream product.

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