A Chinese medicine composition for preventing and treating alcoholic liver damage

Abstract

The present invention provides a Chinese medicine composition for preventing and treating alcoholic liver injury, the composition comprising the following components: Alpinia oxyphylla, Citron, Pueraria root, Ganoderma lucidum, White chrysanthemum, Prunella vulgaris, Imperata root, Lophatherum gracile and Perilla frutescens. Pharmacodynamic experiments have shown that the composition of the present invention has a good liver protection effect, has a significant effect on liver injury, especially alcoholic liver injury, and can be prepared into a medicine or health food with a good preventive and therapeutic effect on acute and chronic hepatitis caused by alcohol.

Description

Technical Field

[0001] The invention belongs to the field of medicines, and particularly relates to a traditional Chinese medicine composition for preventing and treating alcoholic liver damage. Background Art

[0002] The liver is the largest gland in the human body. It plays a very important role in human metabolism, bile production, detoxification, coagulation, immunity, heat production, and the regulation of water and electrolytes. Generally speaking, there are two main pathogenic factors that cause liver damage. One is liver inflammation caused by pathogenic microorganisms such as viral infection, such as hepatitis A and hepatitis B. The other is chemical liver damage, which is caused by various toxic substances, such as alcohol in food, chemical toxins in the environment, and certain drugs. Alcoholic liver damage refers to a series of lesions such as liver damage caused by excessive alcohol intake. Alcoholic liver damage can be divided into acute alcoholic liver damage and chronic alcoholic liver damage. Acute alcoholic liver damage is caused by liver damage due to excessive alcohol intake at one time, manifested as loss of appetite, nausea, liver discomfort, jaundice and other symptoms similar to acute hepatitis. Chronic alcoholic liver damage is caused by long-term alcohol intake, which leads to fibrosis, degeneration and necrosis of liver cells, and the liver's immune function is destroyed. It is often manifested as alcoholic fatty liver, alcoholic hepatitis and alcoholic cirrhosis. The main drugs for treating alcoholic liver damage include: anti-endotoxin drugs, antioxidants, glucocorticoids, and drugs that counteract and improve ethanol metabolism. These drugs have certain effects on acute alcoholic liver damage and the early stage of chronic alcoholic liver damage, but in the late stage of alcoholic liver damage, the human immune function is seriously reduced and the liver function is seriously damaged. Only liver transplantation can significantly improve symptoms and increase survival rate. In short, modern medicine does not have a specific drug for the treatment of acute and chronic alcoholic liver damage.

[0003] Long-term drinking can hinder the decomposition of fatty acids, causing neutral fat accumulation. + It can hinder the decomposition of fatty acids used as energy in the mitochondria of liver cells, causing fatty liver. The fattyization of liver cells seriously affects the normal function of the liver, inhibits the tricarboxylic acid cycle in the body, leads to the obstruction of the metabolic pathway of alcohol, aggravates the damage of alcohol to normal tissues, and forms a vicious cycle. Therefore, the study of a drug that can reduce alcohol toxicity and have a restorative effect on alcohol-induced liver damage has great social benefits and application prospects. Summary of the invention

[0004] In view of the deficiencies in the prior art, the present invention provides a composition for preventing and treating alcoholic liver damage.

[0005] The technical scheme of the present invention is as follows: a Chinese medicine composition for preventing and treating alcoholic liver damage, which comprises the following components by weight: 100-500 parts of Alpinia oxyphylla, 100-500 parts of Pueraria root, 80-200 parts of Citron, 80-200 parts of Ganoderma lucidum, 80-200 parts of White Chrysanthemum, 80-200 parts of Selfheal, 30-60 parts of Imperata root, 30-60 parts of Lophatherum gracile and 10-30 parts of Perilla frutescens.

[0006] Preferably, the Chinese medicine composition comprises the following components by weight: 150-300 parts of Alpinia oxyphylla, 150-350 parts of Pueraria root, 90-200 parts of Citron, 90-150 parts of Ganoderma lucidum, 90-200 parts of White Chrysanthemum, 90-180 parts of Prunella Vulgaris, 30-50 parts of Imperata root, 40-50 parts of Lophatherum gracile and 15-25 parts of Perilla frutescens.

[0007] Preferably, the Chinese medicine composition further comprises 350 parts of sweetener and 0.5 parts of preservative, wherein the flavoring agent is selected from sucrose, and the preservative is selected from ethyl paraben, benzoic acid or sorbic acid.

[0008] Preferably, the Chinese medicine composition comprises the following components by weight: 150g of Alpinia oxyphylla, 150g of Pueraria root, 110g of Citron, 110g of Ganoderma lucidum, 110g of White Chrysanthemum, 110g of Prunella Vulgaris, 50g of Imperata root, 50g of Lophatherum gracile, 20g of Perilla frutescens, 350g of sucrose and 0.5g of ethylparaben.

[0009] Preferably, the Chinese medicine composition comprises the following components by weight: 180g of Alpinia oxyphylla, 180g of Pueraria root, 90g of Citron, 90g of Ganoderma lucidum, 90g of White Chrysanthemum, 90g of Prunella Vulgaris, 40g of Imperata root, 40g of Lophatherum gracile and 15g of Perilla frutescens, 350g of sucrose and 0.5g of benzoic acid.

[0010] Preferably, the Chinese medicine composition comprises the following components by weight: 300g of Alpinia oxyphylla, 350g of Pueraria root, 200g of Citron, 150g of Ganoderma lucidum, 200g of White Chrysanthemum, 180g of Prunella Vulgaris, 30g of Imperata root, 40g of Lophatherum gracile, 25g of Perilla frutescens, 350g of sucrose and 0.5g of sorbic acid.

[0011] In another aspect, the present invention provides a method for preparing the Chinese medicine composition, comprising the following steps:

[0012] 1) Alpinia oxyphylla, 3 kudzu roots, citron, ganoderma lucidum, white chrysanthemum, selfheal, cogongrass root, bamboo leaf and perilla Chinese medicinal slices, add water and boil twice, add 5 times the amount of water for the first time, boil for 60 minutes, and pass the juice through a 150-mesh sieve, add 3 times the amount of water for the second time, boil for 45 minutes, pass the juice through a 150-mesh sieve, combine the two juices, concentrate to 2000 ml, and let stand;

[0013] 2) Take the supernatant and pass it through a 150-mesh sieve and concentrate it to 1000 ml;

[0014] 3) Heat 180 ml of boiling water to dissolve the sucrose, pass through a 150-mesh sieve, add to the drug solution, and continue to concentrate to 1000 ml;

[0015] 4) Take sorbic acid, dissolve it in an appropriate amount of 95% ethanol, add it to the drug solution, stir evenly, boil, hot fill, and sterilize.

[0016] The invention discloses an application of the Chinese medicine composition in preparing medicine for preventing and treating alcoholic liver damage.

[0017] The beneficial effects of the invention are as follows: the compatibility of the Chinese medicine composition complies with the principle of "monarch, minister, assistant and envoy", the combination of the medicines has a good liver-protecting effect, and has a significant effect on liver damage, especially alcoholic liver damage, and can be prepared into a medicine or health food with a good preventive and therapeutic effect on acute and chronic alcoholic hepatitis. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 Cell survival under different ethanol concentrations

[0019] Figure 2 Cell survival under different concentrations of Chinese medicine combination

[0020] Figure 3 Effect of Chinese herbal medicine combination on the prevention and treatment of alcoholic hepatocyte damage

[0021] Figure 4 Evaluation of the therapeutic effect of traditional Chinese medicine combination on hepatocyte injury by oil red staining

[0022] Figure 5 HE staining to evaluate the therapeutic effect of Chinese medicine combination on acute alcoholic liver injury model in rats

[0023] Figure 6 Evaluation of the therapeutic effect of Chinese medicine composition on acute alcoholic liver injury model in rats by oil red staining DETAILED DESCRIPTION

[0024] The following embodiments can enable those skilled in the art to more fully understand the present invention, but the present invention is not limited to the scope of the embodiments.

[0025] Example 1

[0026] 150g of Alpinia oxyphylla, 150g of Pueraria root, 110g of Citron, 110g of Ganoderma lucidum, 110g of White Chrysanthemum, 110g of Prunella vulgaris, 50g of Imperata root, 50g of Light Bamboo Leaf and 20g of Perilla are decocted twice with water. For the first time, 5 times the amount of water is added to boil, and the boiling is kept for 60 minutes. The juice is passed through a 120-mesh sieve. For the second time, 3 times the amount of water is added to boil, and the boiling is kept for 45 minutes. The juice is passed through a 150-mesh sieve. The two juices are combined, concentrated to 2000ml, and allowed to stand. The supernatant is passed through a 150-mesh sieve and concentrated to 1000ml. 350g of sucrose is heated and dissolved with 180ml of boiling water, passed through a 150-mesh sieve, added to the liquid medicine, and continued to be concentrated to 1000ml; 0.5g of ethyl paraben is dissolved with an appropriate amount of 95% ethanol, added to the liquid medicine, stirred evenly, boiled, hot-filled, and sterilized.

[0027] Example 2

[0028] 180g of Alpinia oxyphylla, 180g of Pueraria root, 90g of Citron, 90g of Ganoderma lucidum, 90g of White Chrysanthemum, 90g of Prunella vulgaris, 40g of Imperata root, 40g of Light Bamboo Leaf and 15g of Perilla are decocted twice with water. For the first time, add 5 times the amount of water to boil, keep it for 60 minutes after boiling, and pass the juice through a 150-mesh sieve. For the second time, add 3 times the amount of water to boil, keep it for 45 minutes after boiling, pass the juice through a 150-mesh sieve, combine the two juices, concentrate to 2000ml, and let it stand. Take the supernatant and pass it through a 150-mesh sieve, and concentrate it to 1000ml. Take 350g of sucrose, heat it with 180ml of boiling water to dissolve it, pass it through a 150-mesh sieve, add it to the liquid medicine, and continue to concentrate it to 1000ml; take 0.5g of benzoic acid, dissolve it with an appropriate amount of 95% ethanol, add it to the liquid medicine, stir evenly, boil, hot fill, and sterilize.

[0029] Example 3

[0030] 300g of Alpinia oxyphylla, 350g of Pueraria root, 200g of Citron, 150g of Ganoderma lucidum, 200g of White Chrysanthemum, 180g of Prunella vulgaris, 30g of Imperata root, 40g of Light Bamboo Leaf and 25g of Perilla frutescens are decocted twice with water. For the first time, 5 times the amount of water is added to boil, and the boiling is kept for 60 minutes. The juice is passed through a 150-mesh sieve. For the second time, 3 times the amount of water is added to boil, and the boiling is kept for 45 minutes. The juice is passed through a 150-mesh sieve. The two juices are combined, concentrated to 2000ml, and allowed to stand. The supernatant is passed through a 150-mesh sieve and concentrated to 1000ml. 350g of sucrose is heated and dissolved with 180ml of boiling water, passed through a 150-mesh sieve, added to the liquid medicine, and continued to be concentrated to 1000ml; 0.5g of sorbic acid is dissolved with an appropriate amount of 95% ethanol, added to the liquid medicine, stirred evenly, boiled, hot-filled, and sterilized.

[0031] Test Case

[0032] Experimental Materials:

[0033] Cell line information: normal human liver cells HL-7702, human liver cancer cells HepG2.

[0034] Experimental animals: SPF-grade healthy 6-8-week-old SD male rats, weighing approximately 200 g, were provided by Jinan Pengyue Experimental Animal Breeding Co., Ltd.

[0035] Drugs and reagents: Chinese medicine composition (Chinese medicine composition prepared in Example 1), Ganlixin (Zhengda Tianqing Pharmaceutical Group, National Medicine Standard H10940191), Oil Red O Staining Kit (Biyuntian, C0157M), Improved Oil Red O Staining Kit (Solarbio, 1261), Hematoxylin and Eosin (HE) Staining Kit (Solarbio, 1120), CCK8 Kit (Kermei, MC0301), Anhydrous Ethanol (National Medicine, 10009218), 56-degree Liquor (Niulanshan Erguotou).

[0036] Instruments and equipment:

[0037] Carbon dioxide constant temperature incubator (Thermo), low-speed centrifuge (Anting), pipette (Dalong Xingchuang), clean bench (AIRTECH), Biotek microplate reader (Berteng), ordinary fluorescence inverted microscope (Nikon), ordinary upright fluorescence microscope (Nikon), paraffin slicer (Leica), high-pressure steam sterilizer (TOMY).

[0038] 1 In vitro cell experiments

[0039] Experimental methods:

[0040] 1.1 Cell culture

[0041] The cells were cultured in a 37°C incubator containing 5% CO2. The cells were passaged or experimented when they grew to 80% confluence, and the number of generations used was controlled within 15 generations. The culture medium for normal human liver cells HL-7702 was RPMI1640 containing 10% FBS and 1% double antibody, and the culture medium for human liver cancer cells HepG2 was DMEM high glucose medium containing 10% FBS and 1% double antibody.

[0042] 1.2 Alcohol concentration and Chinese medicine composition concentration gradient design and group setting

[0043] Alcohol concentration gradient: 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%. Treat for 24 hours, detect cell proliferation, and find the toxic dose X%.

[0044] The concentration gradient of the Chinese medicine composition is: 0.25%, 0.5%, 1%, 2%, 4%, 6%, 8%, 10%, 11%, 12%. After 24 hours of treatment, the cell proliferation is detected and the non-toxic dose Y% is explored.

[0045] Under the conditions of cell alcohol toxicity dose X% and non-toxic dose of Chinese herbal composition Y%, control group and experimental group were set up: (1) blank control group; (2) alcohol treatment group; (3) alcohol + Chinese herbal composition group; after 24 hours of treatment, cell proliferation and oil red staining were detected.

[0046] 1.3 CCK8 cell activity detection

[0047] (1) Preparation of CCK8 reaction solution: Mix CCK8 stock solution and preheated culture medium in a ratio of 1:9 for later use.

[0048] (2) Aspirate the culture medium in the 96-well plate and wash the cells with 1× PBS.

[0049] (3) Add 100 μl of the prepared CCK8 reaction solution into each well of a 96-well plate.

[0050] (4) After incubation in the cell culture incubator for 1 hour, the absorbance at a wavelength of 450 nm was measured using an ELISA reader.

[0051] 1.4 Cell Oil Red Staining Method

[0052] (1) Slowly aspirate the cell culture medium and wash once with PBS.

[0053] (2) Add 4% paraformaldehyde fixative for 10 minutes and rinse twice with PBS.

[0054] (3) Add an appropriate amount of staining and washing solution to cover the cells for 20 seconds.

[0055] (4) Aspirate the staining wash solution, add an appropriate amount of Oil Red O staining working solution, and stain for 10-20 minutes.

[0056] (5) Remove the Oil Red O staining solution, add an appropriate amount of staining wash solution, let stand for 30 seconds, then remove the staining wash solution and wash with PBS for 20 seconds.

[0057] (6) Add an appropriate amount of PBS to evenly cover the cells and observe and take pictures under a microscope.

[0058] Statistical methods:

[0059] The experimental data were analyzed and plotted using GraphPadPrism8, and the data were displayed as mean ± SD. The differential expression analysis between the two groups of data was calculated using the Student's test method. P < 0.05 was considered to be significantly different, * was used to indicate P < 0.05, *** was used to indicate P < 0.0005, and **** or #### was used to indicate P < 0.0001. Experimental results:

[0060] 1.5 In vitro cell experiment results

[0061] In order to construct an in vitro model of alcoholic liver injury, the CCK8 cell activity detection experiment was used to explore the ethanol concentration that can cause cytotoxicity in HL-7702 and HepG2 cells. Figure 1 As shown, compared with the control group, 5% and 6% ethanol concentrations were able to cause cell damage in HL-7702 and HepG2 cells, respectively.

[0062] In order to evaluate the therapeutic effect of the Chinese medicine composition on alcoholic liver injury in vitro, the CCK8 cell activity detection experiment was used to explore the concentration of the Chinese medicine composition that does not affect cell activity in HL-7702 and HepG2 cells for subsequent experiments. Figure 2 As shown, 0.25%, 0.5%, and 1% of the Chinese medicine composition concentration had no significant effect on the activity of HL-7702 cells, and 1%, 2%, and 4% of the Chinese medicine composition concentration had no significant effect on the activity of HepG2 cells.

[0063] Based on the exploration of the concentrations of ethanol and Chinese medicine compositions, further CCK8 cell activity detection experiments were conducted.

[0064] The therapeutic effect of the Chinese herbal medicine composition on alcoholic liver cell injury was evaluated. Figure 3 As shown, compared with the control group, ethanol treatment can significantly inhibit cell activity, while the Chinese medicine composition can restore cell activity in a concentration gradient-dependent manner with significant statistical differences.

[0065] Fatty degeneration and lipid droplet accumulation are prominent features of alcoholic hepatocyte injury. The therapeutic effect of the Chinese medicine composition on hepatocyte injury was evaluated by further oil red staining. The staining results are as follows Figure 4 As shown, ethanol treatment not only affects cell activity, but also causes significant lipid accumulation in cells, and the Chinese herbal composition can significantly alleviate the lipid accumulation caused by ethanol, indicating the protective effect of the Chinese herbal composition on alcoholic liver damage.

[0066] 2 In vivo animal experiments

[0067] 2.1 Rat husbandry

[0068] Healthy 6-8 week old SD rats were provided by Jinan Pengyue Experimental Animal Breeding Co., Ltd. and were kept in an environment with a temperature of 20℃-24℃, a humidity of 40%-70%, a wind speed of 0.1M / s-0.2M / s, and an airflow of ≥28 times / hour. During the experiment, the rats were free to eat and drink.

[0069] 2.2 Rat modeling and grouping

[0070] Induction of acute alcoholic liver injury model:

[0071] The rats were gavaged with 56-proof liquor, 7 ml / kg each time, twice a day, once in the morning and once in the evening, for 2 weeks, and the body weight was recorded daily.

[0072] Group settings:

[0073] (1) Three rats were fed a normal diet;

[0074] (2) 3 rats were gavaged with liquor for 2 weeks;

[0075] (3) 3 rats were gavaged with the Chinese medicine combination for 2 weeks;

[0076] (4) 3 rats were given oral administration of liquor (first) + oral administration of Chinese medicine combination (latter), with a half-hour interval;

[0077] (5) 3 rats were gavaged with Chinese medicine combination (first) and liquor (later) with a half-hour interval;

[0078] (6) 3 rats were given liquor orally for 2 weeks and Chinese medicine combination for 1 week;

[0079] (7) 3 rats were given liquor orally for 2 weeks and glycyrrhizin for 1 week;

[0080] 2.3 Dosage of Chinese medicine composition and positive drug (Glycyrrhizin)

[0081] Chinese medicine composition: After the Chinese medicine composition is concentrated 1.8 times, the single dosage for rats is 1 ml.

[0082] Ganlixin: The single dose for rats is 2.7 mg. Each capsule can be given to 18.5 rats. It is diluted to 18.5 ml with normal saline and 1 ml is given each time.

[0083] 2.4 Tissue HE staining and oil red staining methods

[0084] HE staining:

[0085] (1) Freshly collected samples were fixed, embedded in conventional paraffin, and sliced ​​into 3-8 μm sections;

[0086] (2) Dewax in xylene twice, 5-10 min each time, rehydrate in a series of ethanol (100%, 95%, 85%, 75%), 3 min each gradient. Soak in distilled water for 2 min;

[0087] (3) Stain with hematoxylin for 2-20 min and wash with distilled water to remove floating color;

[0088] (4) Differentiation solution was used for 10-60 seconds, and tap water was added or rinsed twice, each time for 3-5 minutes;

[0089] (5) Place in eosin stain solution for 30 seconds to 2 minutes, pour off excess stain solution and dehydrate quickly;

[0090] (6) Dehydration, transparency, and sealing: 75%, 85%, 95%, and 100% ethanol, each for 2-3 seconds, 100% ethanol

[0091] Soak for 1 min, clear with xylene twice, 1 min each time, seal with neutral gum, and observe under a microscope.

[0092] Oil red staining:

[0093] (1) Frozen sections were 6-10 μm thick and were left unfixed or fixed with 10% formalin for 10 min and then washed with water;

[0094] (2) Rinse the slices briefly in distilled water;

[0095] (3) Immerse the slices in 60% isopropanol for 20-30 seconds;

[0096] (4) Slice into modified Oil Red O staining solution (covered) and stain for 10-15 min;

[0097] (5) Color separation: Wash briefly in 60% isopropyl alcohol to remove the dye;

[0098] (6) Rinse briefly in distilled water;

[0099] (7) Mayer's hematoxylin staining solution counterstains the nuclei for 1-2 min;

[0100] (8) 1% hydrochloride solution is slightly differentiated;

[0101] (9) Rinse with tap water for 10 minutes or induce blueing in dilute lithium carbonate solution;

[0102] (10) Wash lightly in distilled water;

[0103] (11) Use filter paper to absorb the surrounding water;

[0104] (12) Sealing with glycerol gelatin or gum arabic.

[0105] Statistical methods:

[0106] The experimental data were analyzed and plotted using GraphPadPrism8, and the data were displayed as mean ± SD. The differential expression analysis between the two groups of data was calculated using the Student's test method. P < 0.05 was considered to be significantly different, * was used to indicate P < 0.05, *** was used to indicate P < 0.0005, and **** or #### was used to indicate P < 0.0001. Experimental results:

[0107] 2.5 In vivo animal experiment results

[0108] The effect of the Chinese medicine composition on alcoholic liver injury was evaluated using an in vivo model of acute alcoholic liver injury in rats, and was evaluated by HE staining and oil red staining, respectively. The results of the HE experiment are as follows: Figure 5 As shown in Figure 2, in the acute alcoholic liver injury model, compared with the control group, 56% liquor can cause obvious fat cavitation, while the Chinese medicine composition treatment group and the glycyrrhizin treatment group had no significant changes. Figure 6 As shown, compared with the control group, the white wine group rats had obvious lipid accumulation in the liver, and the Chinese medicine composition and glycyrrhizin administration groups were able to significantly alleviate the lipid accumulation caused by white wine, which preliminarily suggested that the Chinese medicine composition had a certain preventive and therapeutic effect on alcoholic liver injury.

[0109] Experimental discussion and conclusion:

[0110] According to the analysis of the prescription compatibility of the Chinese medicine composition, the main ingredients of the Chinese medicine composition are kudzu root, yi xiao ren, ganoderma lucidum, white chrysanthemum, selfheal, bergamot, white cypress root, bamboo leaf and perilla. Kudzu root and yi xiao ren are the monarch, belonging to the liver, kidney and spleen meridians, and have the effect of protecting the liver, strengthening the kidney and strengthening the spleen. Ganoderma lucidum, white chrysanthemum, selfheal, and bergamot are the ministers, which have the effects of clearing away heat and detoxification, clearing the liver and improving eyesight, dispersing stagnation and reducing swelling, soothing the liver and stomach, and regulating immune function, improving the body's disease resistance, and cooperating with the monarch. White cypress root and bamboo leaf are adjuvants, which have the effects of cooling blood and promoting body fluid, clearing heat and promoting diuresis, and eliminating restlessness. Perilla is the guiding drug, which regulates qi and relieves fullness, and harmonizes the stomach. The combination of these drugs plays the role of protecting the liver, nourishing the stomach and refreshing the brain, and should have a good therapeutic effect on liver damage, especially alcoholic liver damage.

[0111] In order to evaluate the effect of Chinese herbal medicine on alcoholic liver injury, this study conducted in vitro cell experiments and in vivo animal experiments. First, using the in vitro HL-7702 and HepG2 alcoholic liver cell injury models, CCK8 cell activity detection experiments and oil red staining experiments preliminarily proved that the Chinese herbal medicine can restore liver cell injury caused by ethanol treatment and relieve lipid accumulation in a concentration gradient-dependent manner. Further, using the in vivo SD rat acute alcoholic liver injury model, HE staining and oil red staining experiments proved that the Chinese herbal medicine has a good preventive and therapeutic effect on acute alcoholic liver injury.

Claims

1. A Chinese medicine composition for preventing and treating alcoholic liver damage, characterized in that: The raw materials are calculated by weight: 100-500 parts of Alpinia oxyphylla, 100-500 parts of Pueraria root, 80-200 parts of Citron, 80-200 parts of Ganoderma lucidum, 80-200 parts of White Chrysanthemum, 80-200 parts of Prunella Vulgaris, 30-60 parts of Imperata root, 30-60 parts of Lophatherum gracile and 10-30 parts of Perilla frutescens.

2. The Chinese medicine composition according to claim 1, characterized in that: The raw materials are calculated by weight: 150-300 parts of Alpinia oxyphylla, 150-350 parts of Pueraria root, 90-200 parts of Citron, 90-150 parts of Ganoderma lucidum, 90-200 parts of White Chrysanthemum, 90-180 parts of Prunella Vulgaris, 30-50 parts of Imperata root, 40-50 parts of Lophatherum gracile and 15-25 parts of Perilla frutescens.

3. The Chinese medicine composition according to claim 1, characterized in that The Chinese medicine composition also includes 350 parts of sweeteners and 0.5 parts of preservatives, wherein the flavoring agent is selected from sucrose, and the preservative is selected from ethyl paraben, benzoic acid or sorbic acid.

4. The Chinese medicine composition according to claim 1, characterized in that: The invention is prepared from the following raw materials in parts by weight: 150g of Alpinia oxyphylla, 150g of Pueraria root, 110g of Citron, 110g of Ganoderma lucidum, 110g of White Chrysanthemum, 110g of Prunella Vulgaris, 50g of Imperata root, 50g of Lophatherum gracile, 20g of Perilla frutescens, 350g of sucrose and 0.5g of ethylparaben.

5. The Chinese medicine composition according to claim 1, characterized in that: The invention is prepared from the following raw materials in parts by weight: 180g of Alpinia oxyphylla, 180g of Pueraria root, 90g of Citron, 90g of Ganoderma lucidum, 90g of White Chrysanthemum, 90g of Prunella Vulgaris, 40g of Imperata root, 40g of Lophatherum gracile, 15g of Perilla frutescens, 350g of sucrose and 0.5g of benzoic acid.

6. The Chinese medicine composition according to claim 1, characterized in that: The invention is prepared from the following raw materials in parts by weight: 300g of Alpinia oxyphylla, 350g of Pueraria root, 200g of Citron, 150g of Ganoderma lucidum, 200g of White Chrysanthemum, 180g of Prunella Vulgaris, 30g of Imperata root, 40g of Lophatherum gracile, 25g of Perilla frutescens, 350g of sucrose and 0.5g of sorbic acid.

7. A method for preparing the Chinese medicine composition according to any one of claims 4 to 6, comprising the following steps: 1) Add water to the Chinese medicinal pieces of Alpinia oxyphylla, Pueraria root, Citron, Ganoderma lucidum, White chrysanthemum, Prunella vulgaris, Imperata root, Lophatherum gracile and Perilla frutescens and boil them twice. For the first time, add 5 times the amount of water to boil, keep boiling for 60 minutes, and pass the juice through a 150-mesh sieve. For the second time, add 3 times the amount of water to boil, keep boiling for 45 minutes, and pass the juice through a 150-mesh sieve. Combine the two juices, concentrate to 2000 ml, and let stand; 2) Take the supernatant and pass it through a 150-mesh sieve, and concentrate it to 1000 ml; 3) Heat 180ml of boiling water to dissolve the sucrose, pass through a 150-mesh sieve, add to the drug solution, and continue to concentrate to 1000ml; 4) Take sorbic acid, dissolve it in appropriate amount of 95% ethanol, add it to the medicine solution, stir evenly, boil, hot fill, and sterilize.

8. Use of the Chinese medicine composition according to claim 1 in the preparation of medicines for preventing and treating alcoholic liver damage.

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