A biological sunscreen and barrier repair additive composition, preparation method, application and sunscreen value testing method

Abstract

The invention provides a preparation method of a biological sunscreen and barrier repair additive composition and application thereof in sunscreen products; the invention can achieve a sunscreen effect relatively quickly, can not only absorb ultraviolet rays, but also inhibit the activity of tyrosinase, thereby providing sunscreen from a deep level; the composition provided by the invention is not prone to allergies, can be added to an existing sunscreen product, or used independently, and can also be used in sunscreen products for pregnant women and children, with a refreshing and non-greasy skin feel; active ingredients are extracted from two microalgae, Dunaliella salina and Haematococcus sanguinosa, which are rich in several carotenoids and enhance the protection of the skin: resist ultraviolet radiation, target sunburn, resist inflammation, eliminate redness and swelling, and soothe the skin.

Description

Technical Field

[0001] The present invention relates to the technical field of daily chemicals, and in particular to a biological sunscreen and barrier repair additive composition, a preparation method and application thereof. Background Art

[0002] At present, there are three types of sunscreens on the market. The first type is physical sunscreens, such as zinc oxide and titanium dioxide, which mainly use their own physical properties to absorb and reflect ultraviolet rays. During use, they are mainly retained on the skin surface to protect against the sun. And because the microstructure of the skin is a gully-shaped structure, physical sunscreens can only reach a maximum sun protection index of spf20. In order to improve the sunscreen performance of inorganic substances, it is considered to reduce the particle size of inorganic substances to the nanometer level. When at the nanometer level, there is a possibility of being absorbed by the skin, there is a potential risk of allergies, and there is a risk of inhalation for children, so it is banned in children's products; the second type: chemical sunscreens, which are also a type of sunscreen that is often used. Although chemical sunscreen products have excellent and fast sunscreen performance, they will be absorbed by the skin and even enter the blood, which will have certain toxicity and cause certain harm to the human body. In addition, some people are very likely to have allergic symptoms to chemical sunscreens; the third type is biological sunscreens, which are also the latest popular type of sunscreens in recent years. Biological sunscreens themselves 1. have a certain ability to absorb ultraviolet rays. 2. Because ultraviolet radiation is an oxidative stress process that causes a series of tissue damage by producing oxygen free radicals, the substances contained in biological sunscreens can block or slow down tissue damage or promote post-sun repair by removing or reducing intermediate products of oxygen active groups, thus playing an indirect sun protection role.

[0003] Biological sunscreens are a type of sunscreen that is less likely to cause allergies, but due to their mechanism of action and the fact that they cannot be used independently as sunscreens, they are not yet widely used. Summary of the invention

[0004] The purpose of the present invention is to provide a fast, long-lasting and efficient sunscreen composition to solve the technical problem of the contradiction between the sunscreen effect and the usage amount existing in the prior art.

[0005] In order to solve the above technical problems, the present invention provides a biological sunscreen and barrier repair additive composition, which includes glabrous Pongamia seed oil, Dunaliella salina extract, Haematococcus pluvialis extract, and Porphyra umbilicus extract; and the ratio of the Dunaliella salina extract, Haematococcus pluvialis extract, and Porphyra umbilicus extract is: (10-30):(20-70):(0.1-1).

[0006] Preferably, the extraction method of the Pongamia glabrous seed oil is as follows:

[0007] (1) Pretreatment: collecting Pongamia glabra seeds, placing them in an oven for drying, and grinding the dried Pongamia glabra seeds to obtain Pongamia glabra seed powder;

[0008] (2) Extraction: Place the Pongamia glabra seed powder in an extraction bottle, add a mixed solvent at a solid-liquid ratio of 1:10, and extract at an ultrasonic power of 200 W, an ultrasonic time of 30 min, and an ultrasonic temperature of 60° C. After extraction, filter to obtain a filtrate and a filter residue respectively;

[0009] (3) mixing the filter residue and the culture medium together and culturing them at 27±1° C. for 7 days, and mixing the obtained culture solution with the filtrate in step (2), wherein the bacterial species is Bacillus subtilis and the inoculation amount is 6%;

[0010] (4) Add an equal volume of deionized water to the mixed solution obtained in step (3) and let it stand, take the upper layer of liquid, and then rotary evaporate it until it becomes a paste.

[0011] Preferably, the temperature in the oven is 80° C.; and the mixed solvent is a mixed solvent of ethanol and n-propanol in a volume ratio of 2:3.

[0012] Preferably, the ratio of the Dunaliella salina extract, the Haematococcus pluvialis extract and the Porphyra navelii extract is: (15-30): (25-70): (0.5-1).

[0013] Preferably, the ratio of the Dunaliella salina extract, the Haematococcus pluvialis extract and the Porphyra navelii extract is: (20-30): (40-70): (0.5-1).

[0014] Preferably, the composition further comprises milk thistle seed oil and Nannochloropsis oculata extract.

[0015] Preferably, the composition is added to daily chemicals at a concentration of 0.1-5 wt %.

[0016] Another aspect of the present invention provides a method for preparing the bio-based sunscreen and barrier repair additive composition according to claim 6, the method comprising:

[0017] S01: dissolving Pongamia glabrous seed oil, Silybum marianum seed oil and Nannochloropsis oculata extract in ethanol to obtain a mixture 1;

[0018] S02: mixing the remaining raw materials with the lower layer liquid of step (2) to obtain mixture 2;

[0019] S03: Apply mixture 1 to the inner wall of the container, dry it, add mixture 2, and then rotate and shake at 35°C to prepare the product.

[0020] Another aspect of the present invention provides the bio-based sunscreen and barrier repair additive composition for use in the field of sunscreen.

[0021] Another aspect of the present invention provides the biological sunscreen and barrier repair additive composition for application in the field of skin barrier repair.

[0022] Another aspect of the present invention provides a method for testing sun protection values, the steps of which are as follows: applying the composition to a test position, and simultaneously subjecting different test positions of the same subject to gradient ultraviolet irradiation, and calculating the sun protection value after irradiation.

[0023] By adopting the above technical solution, the present invention has the following beneficial effects:

[0024] 1. The present invention can achieve a relatively good sun protection effect, not only absorbing ultraviolet rays, but also inhibiting the activity of tyrosinase, thus providing sun protection from a deep level;

[0025] 2. The composition provided by the present invention is not easy to cause allergies, and can be added to an existing sunscreen product, or used independently. It can also be used in sunscreen products for pregnant women and children, and the skin feels refreshing and non-greasy;

[0026] 3. The active ingredients extracted from Dunaliella salina and Haematococcus sanguineus are rich in several carotenoids, which can enhance the protection of the skin: resist ultraviolet radiation, target sunburn, fight inflammation, eliminate redness and swelling, and soothe the skin. Through its own anti-inflammatory effect, it promotes capillary contraction (strong capillary contraction agent) to delay the appearance of long-lasting erythema on the skin, thereby achieving the effect of increasing the SPF value. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] In order to more clearly illustrate the specific implementation methods of the present invention or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings in the following description are some implementation methods of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0028] Figure 1 It is an SPF test chart (a test comparison chart of Example 2 and Example 6). DETAILED DESCRIPTION

[0029] The technical solution of the present invention will be described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0030] The present invention provides a bio-type sunscreen and barrier repair additive composition, which comprises Pongamia glabrous seed oil, Dunaliella salina extract, Haematococcus pluvialis extract, and Porphyra umbilicus extract; and the proportions of the Dunaliella salina extract, Haematococcus pluvialis extract, and Porphyra umbilicus extract are:

[0031] (10-30):(20-70):(0.1-1).

[0032] Pongamia glabra seed oil: Pongamia glabra seed oil. In the present invention, the extraction method of the Pongamia glabra seed oil is as follows:

[0033] (1) Pretreatment: collecting Pongamia glabrous seeds, placing them in an oven for drying, and grinding the dried Pongamia glabrous seeds to obtain Pongamia glabrous seeds powder; the oven is preheated to 80° C.;

[0034] (2) Extraction: Place the Pongamia pubescens seed powder in an extraction bottle, add a mixed solvent at a solid-liquid ratio of 1:10, and extract at an ultrasonic power of 200 W, an ultrasonic time of 30 min, and an ultrasonic temperature of 60° C. After extraction, filter to obtain a filtrate and a filter residue respectively; wherein the mixed solvent is a mixed solvent of ethanol and n-propanol at a volume ratio of 2:3;

[0035] (3) mixing the filter residue and the culture medium together and culturing them at 27±1° C. for 7 days, and mixing the obtained culture solution with the filtrate in step (2), wherein the bacterial species is Bacillus subtilis and the inoculation amount is 6%;

[0036] The Bacillus subtilis was purchased from China General Microbiological Culture Collection Center with the collection number of 1.1498. The inoculation amount of the filter residue was 20%.

[0037] The components of the culture medium are: glucose 10 g / L, inositol 0.01 g / L, riboflavin 3.5 g / L, vitamin B12 4.2 mg / L, (NH4)2SO4 1 g / L, MgSO4·7H2O 0.2 g / L, NaCl 0.3 / L, K2HPO4 1 g / L, and the balance is water; natural pH.

[0038] (4) Add an equal volume of deionized water to the mixed solution obtained in step (3) and let it stand, take the upper layer of liquid, and then rotary evaporate it until it becomes a paste.

[0039] Dunaliella salina extract: DUNALIELLA SALINA EXTRACT. The Dunaliella salina extract in the present invention is purchased from Shaanxi Haibo Biotechnology Co., Ltd.

[0040] Haematococcus pluvialis extract: The Haematococcus pluvialis extract in the present invention was purchased from Xi'an Muyuan Biotechnology Co., Ltd.

[0041] Porphyra umbilicalis extract: Porphyra umbilicalis extract, which was purchased from Kangdu Pharmaceutical Co., Ltd.

[0042] Preferably, the ratio of the Dunaliella salina extract, the Haematococcus pluvialis extract and the Porphyra navelii extract is: (15-30): (25-70): (0.5-1).

[0043] More preferably, the ratio of the Dunaliella salina extract, the Haematococcus pluvialis extract and the Porphyra navelii extract is: (20-30): (40-70): (0.5-1).

[0044] Further preferably, the composition further comprises milk thistle seed oil and Nannochloropsis oculata extract.

[0045] In the present invention, the silybum seed oil is purchased from Shanxi Wenyun Biotechnology Co., Ltd.; the Nannochloropsis oculata extract, i.e., the Nannochloropsis oculata extract is purchased from Wuhan Jiuzhouyuan Biotechnology Co., Ltd.

[0046] In the present invention, the biological sunscreen and barrier repair additive composition is added to daily chemical products at a concentration of 0.1-5wt%.

[0047] Another aspect of the present invention provides a method for preparing the bio-based sunscreen and barrier repair additive composition, the method comprising:

[0048] S01: dissolving Pongamia glabrous seed oil, Silybum marianum seed oil and Nannochloropsis oculata extract in ethanol to obtain a mixture 1;

[0049] S02: mixing the remaining raw materials with the lower layer liquid of step (2) to obtain mixture 2;

[0050] S03: Apply mixture 1 to the inner wall of the container, dry it, add mixture 2, and then rotate and shake at 35°C to prepare the product.

[0051] Another aspect of the present invention provides the bio-based sunscreen and barrier repair additive composition for use in the field of sunscreen.

[0052] Another aspect of the present invention provides the biological sunscreen and barrier repair additive composition for application in the field of skin barrier repair.

[0053] The inventors of the present application have found in their research that although the combination of Dunaliella salina extract, Haematococcus pluvialis extract and Porphyra umbilicus extract has certain sunscreen performance, it is found that the three can only play a sunscreen performance in a certain ratio. The possible reason is that the Dunaliella salina extract, Haematococcus pluvialis extract and Porphyra umbilicus extract can inhibit tyrosinase, but when the ratio of the three exceeds a certain range, competitive inhibition will occur between the three, so the sunscreen effect will be reduced. The inventors can solve the above problems by adding Pongamia glabrous seed oil to the composition. The possible reason is that the parent ring of the sunscreen component in Pongamia glabrous seed oil is benzopyran, and the above contains polyhydroxyl groups, which causes certain steric hindrance, so that the above three components can be balanced, and the ratio range between the three components is expanded at the same time, which greatly reduces the cost and allergy possibility.

[0054] The present invention is further explained below in conjunction with specific implementation modes.

[0055] Example 1

[0056] The present embodiment provides a biological sunscreen and barrier repair additive composition, which includes Pongamia glabrous seed oil, Dunaliella salina extract, Haematococcus pluvialis extract, Porphyra umbellata extract, Silybum marianum seed oil, and Nannochloropsis oculata extract; and the mass ratio is 3:23:55:0.7:8:0.1.

[0057] Wherein, the extraction method of the Pongamia glabrous seed oil is as follows:

[0058] (1) Pretreatment: collecting Pongamia glabrous seeds, placing them in an oven for drying, and grinding the dried Pongamia glabrous seeds to obtain Pongamia glabrous seeds powder; the oven is preheated to 80° C.;

[0059] (2) Extraction: Place the Pongamia pubescens seed powder in an extraction bottle, add a mixed solvent at a solid-liquid ratio of 1:10, and extract at an ultrasonic power of 200 W, an ultrasonic time of 30 min, and an ultrasonic temperature of 60° C. After extraction, filter to obtain a filtrate and a filter residue respectively; wherein the mixed solvent is a mixed solvent of ethanol and n-propanol at a volume ratio of 2:3;

[0060] (3) mixing the filter residue and the culture medium together and culturing them at 27±1° C. for 7 days, and mixing the obtained culture solution with the filtrate in step (2), wherein the bacterial species is Bacillus subtilis and the inoculation amount is 6%;

[0061] The Bacillus subtilis was purchased from China General Microbiological Culture Collection Center with the collection number of 1.1498. The inoculation amount of the filter residue was 20%.

[0062] The components of the culture medium are: glucose 10 g / L, inositol 0.01 g / L, riboflavin 3.5 g / L, vitamin B12 4.2 mg / L, (NH4)2SO4 1 g / L, MgSO4·7H2O 0.2 g / L, NaCl 0.3 / L, K2HPO4 1 g / L, and the balance is water; natural pH.

[0063] (4) Add an equal volume of deionized water to the mixed solution obtained in step (3) and let it stand, take the upper layer of liquid, and then rotary evaporate it until it becomes a paste.

[0064] The Dunaliella salina extract was purchased from Shaanxi Haibo Biotechnology Co., Ltd.

[0065] The Haematococcus pluvialis extract was purchased from Xi'an Muyuan Biotechnology Co., Ltd.

[0066] PORPHYRA UMBILICALIS extract was purchased from Kangdu Pharmaceutical Co., Ltd.

[0067] The silymarin seed oil was purchased from Shanxi Wenyun Biotechnology Co., Ltd.

[0068] The extract of Nannochloropsis oculata was purchased from Wuhan Jiuzhouyuan Biotechnology Co., Ltd.

[0069] Another aspect of this embodiment provides a method for preparing the bio-based sunscreen and barrier repair additive composition, the method comprising:

[0070] S01: dissolving Pongamia glabrous seed oil, Silybum marianum seed oil and Nannochloropsis oculata extract in ethanol to obtain a mixture 1;

[0071] S02: mixing the remaining raw materials with the lower layer liquid of step (2) to obtain mixture 2;

[0072] S03: Apply mixture 1 to the inner wall of the container, dry it, add mixture 2, and then rotate and shake at 35°C to prepare the product.

[0073] Example 2

[0074] The present embodiment provides a biological sunscreen and barrier repair additive composition, which includes Pongamia glabrous seed oil, Dunaliella salina extract, Haematococcus pluvialis extract, Porphyra umbellata extract, Silybum marianum seed oil, and Nannochloropsis oculata extract; and the mass ratio is 3:23:55:0.7:8:0.1.

[0075] Wherein, the extraction method of the Pongamia glabrous seed oil is as follows:

[0076] (1) Pretreatment: collecting Pongamia glabrous seeds, placing them in an oven for drying, and grinding the dried Pongamia glabrous seeds to obtain Pongamia glabrous seeds powder; the oven is preheated to 80° C.;

[0077] (2) Extraction: Place the Pongamia glabrous seed powder in an extraction bottle, add a mixed solvent at a solid-liquid ratio of 1:10, and extract at an ultrasonic power of 200 W, an ultrasonic time of 30 min, and an ultrasonic temperature of 60°C. After extraction, filter to obtain a filtrate and a filter residue respectively; wherein the mixed solvent is a mixed solvent of ethanol and n-propanol in a volume ratio of 2:3; and rotary evaporate the filtrate until it becomes a paste.

[0078] The Dunaliella salina extract was purchased from Shaanxi Haibo Biotechnology Co., Ltd.

[0079] The Haematococcus pluvialis extract was purchased from Xi'an Muyuan Biotechnology Co., Ltd.

[0080] PORPHYRA UMBILICALIS extract was purchased from Kangdu Pharmaceutical Co., Ltd.

[0081] The silymarin seed oil was purchased from Shanxi Wenyun Biotechnology Co., Ltd.

[0082] The extract of Nannochloropsis oculata was purchased from Wuhan Jiuzhouyuan Biotechnology Co., Ltd.

[0083] Another aspect of this embodiment provides a method for preparing the bio-based sunscreen and barrier repair additive composition, the method comprising:

[0084] S01: dissolving Pongamia glabrous seed oil, Silybum marianum seed oil and Nannochloropsis oculata extract in ethanol to obtain a mixture 1;

[0085] S02: mixing the remaining raw materials with the lower layer liquid of step (2) to obtain mixture 2;

[0086] S03: Apply mixture 1 to the inner wall of the container, dry it, add mixture 2, and then rotate and shake at 35°C to prepare the product.

[0087] Example 3

[0088] The difference from Example 1 is that the preparation method of the composition is as follows: S01 dissolving Pongamia glabrous seed oil, Silybum marianum seed oil, and Nannochloropsis eye-veined extract in ethanol to obtain a mixture 1;

[0089] S02: mixing the remaining raw materials with the lower layer liquid of step (2) to obtain mixture 2;

[0090] S03 Mixture 1 and mixture 2 with stirring at 300 r / min for 5 min.

[0091] Example 4

[0092] The difference from Example 1 is that the composition does not contain Pongamia glabrous seed oil.

[0093] Example 5

[0094] The difference from Example 1 is that, when the total amount of Dunaliella salina extract, Haematococcus pluvialis extract and Porphyra umbilicus extract remains unchanged, the ratio of the Dunaliella salina extract, Haematococcus pluvialis extract and Porphyra umbilicus extract is adjusted to 10:20:0.1.

[0095] Example 6

[0096] The difference from Example 1 is that, when the total amount of Dunaliella salina extract, Haematococcus pluvialis extract and Porphyra umbilicus extract remains unchanged, the ratio of the Dunaliella salina extract, Haematococcus pluvialis extract and Porphyra umbilicus extract is adjusted to 30:70:11.

[0097] Example 7

[0098] The difference from Example 5 is that the Pongamia glabrous seed oil is not contained.

[0099] Example 8

[0100] The difference from Example 6 is that the Pongamia glabrous seed oil is not contained.

[0101] Performance testing: Basic testing methods:

[0102] PFA test: The test is carried out in accordance with the specific requirements of the currently valid technical specifications. The subject is in a forward leaning position and the back is irradiated. The minimum blackening amount (MPPD value) of the subject's skin to ultraviolet radiation is predicted 24 hours before the test, and the ultraviolet radiation amount is adjusted according to the predicted results for the test object. The test first selects a spot no less than 30cm on the subject's back. 2 For normal skin area, (2.00±0.05)mg / cm 2 Apply the test object or reference product evenly on the above area with the dosage; then select the UVA exposure amount according to the specification requirements, and irradiate in three situations: ① The test object is not applied to the subject's skin; ② The reference product is applied; ③ The test object is applied. Observe the experimental results after 2 hours and record the MPPD values ​​in the three situations respectively.

[0103] Calculation method of PFA value: The PFA value of the test substance or reference substance protecting a single subject is expressed as follows:

[0104]

[0105] Individual PFA values ​​are required to be accurate to one decimal place. The arithmetic mean of the PFA values ​​of all subjects of the measured object protection is calculated, and the integer part is the PFA value of the measured sample. The sampling error of the estimated mean can calculate the standard deviation and standard error of the group of data. The 95% confidence interval (95% CI) of the mean is required to be no more than 17% of the mean. The number of subjects is increased until the above requirements are met, but it shall not exceed 25 people.

[0106] SPF test: The test is carried out in accordance with the specific requirements of the currently valid technical specifications. The subject is in a forward-leaning position and the back is exposed to the radiation. The minimum erythema dose (MED value) of the subject's skin to ultraviolet radiation is predicted 24 hours before the test, and the ultraviolet radiation dose is adjusted according to the predicted results for the test object. On the day of the test, a spot no less than 30cm on the subject's back is first selected. 2 Normal skin area, (2.00±0.05)mg / cm 2 The test object or reference substance is evenly applied to the above-mentioned area with the dosage of 100 mg / kg. Then the irradiation dose is selected according to the specification requirements, and irradiation is performed in three conditions: ① The test object is not applied to the subject's skin; ② The reference substance is applied; ③ The test object is applied. Observe the experimental results after 24 hours and record the MED values ​​in the three conditions respectively.

[0107] SPF value calculation method: The SPF value of the test object or reference product protecting a single subject is expressed as follows:

[0108]

[0109] The individual SPF value is required to be accurate to one decimal place. The arithmetic mean of the SPF values ​​of all subjects protected by the measured object is calculated, and the integer part is the SPF value of the measured sample. The sampling error of the estimated mean can be used to calculate the standard deviation and standard error of the data set. The 95% confidence interval (95% CI) of the mean is required to not exceed 17% of the mean, otherwise the number of subjects should be increased (not more than 25) until the above requirements are met.

[0110] Among them, in performance test 1 and performance test 2:

[0111] The object to be tested is the sunscreen composition prepared in the examples.

[0112] Light source: Daylight simulator xenon arc lamp, all performance indicators meet the measurement specification requirements.

[0113] The control product is a standard product, SPF=20, PFA=8; during the test, the composition prepared in 1-8 is added to the standard product at a concentration of 0.5%, and is used as the test product 1-8.

[0114] Test 1: Apply the composition of test products 1-8 respectively until 15 minutes after skin absorption, and then conduct the above-mentioned PFA and SPF tests; among them, 3 additional groups of tests are added: after test products 4, 7, and 8 are applied and absorbed, an equal amount of Pongamia glabrous seed oil as that of test product 1 is additionally applied, and the test is conducted again after absorption, which are designated as test products 9-11.

[0115] Test 2: The above-mentioned PFA and SPF tests were immediately performed after applying the compositions of test products 1-8 respectively; among them, 3 additional groups of tests were added: after test products 4, 7, and 8 were applied and absorbed, an additional amount of Pongamia glabrous seed oil equal to that of test product 1 was applied, and the test was performed again after absorption, which were designated as test products 9-11.

[0116] Subjects: A total of 36 people were randomly divided into 12 groups, with an average age of 33.5±4.2 years old, meeting the voluntary selection criteria. Four groups of test products were tested at the same time for each person, and each test product or standard product was tested 12 times.

[0117] Table 1 Test results of performance tests 1 and 2

[0118]

[0119]

[0120] Among them, Figure 1 Shown are the test comparison diagrams of Example 2 and Example 6 in the SPF test diagram, wherein MED is the initial test diagram for determining the degree to which the subject's body can withstand ultraviolet rays; 0240 is the test diagram of Example 2, 0241 is the test diagram of Example 6; and P2 is the positive control diagram.

[0121] Performance test 3: Kojic acid was used as the positive control and diluted with pure water to a positive control concentration of 0.1%. The negative control was pure water. The test product: Example 1-8 was diluted with 95% ethanol to a test concentration of 0.2%.

[0122] Operation steps: Set up different reaction tubes, namely sample tube, sample background tube, enzyme reaction tube and solvent background tube, add different reagent solvents respectively, shake gently, let stand at room temperature for 5 minutes, transfer the reaction solution into a 1 cm cuvette, and measure the absorbance at 475 nm.

[0123] Calculation of tyrosinase inhibition rate (%) = (1-(T-T0) / (C-C0)) × 100

[0124] in:

[0125] T-absorbance value of the solution after the test product reacts with tyrosinase;

[0126] T0-background absorbance of the test product;

[0127] C- the average of 3 absorbance values ​​of the enzyme reaction tube, i.e. the absorbance value of the tyrosinase and DOPA reaction without adding the test sample;

[0128] C0-solvent background absorbance.

[0129] The test results are as follows:

[0130] Table 2 Test results of performance test 3

[0131]

[0132]

[0133] Among them, the tyrosinase inhibition rate is retained to three decimal places, and P<0.05 indicates that there are significant differences between the sample and the positive control and the negative control.

[0134] Test 4: Statistics of the test subjects’ experience with the test product in Test 1.

[0135] It is divided into: refreshing 5 points, moderate 3 points, greasy 1 point.

[0136] Table 3 Test results of performance test 4

[0137] Example Test 4 Results Example 1 15 Example 2 13 Example 3 11 Example 4 11 Example 5 13 Example 6 11 Example 7 9 Example 8 9 Example 9 11 Example 10 7 Embodiment 11 7

[0138] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A biological sunscreen and barrier repair additive composition, characterized in that: The composition comprises Pongamia glabrous seed oil, Dunaliella salina extract, Haematococcus pluvialis extract, Porphyra umbellata extract, Silybum marianum seed oil, and Nannochloropsis oculata extract; and the mass ratio is 3:23:55:0.7:8:0.1; Wherein, the extraction method of the Pongamia glabrous seed oil is as follows: (1) Pretreatment: collecting Pongamia glabrous seeds, placing them in an oven for drying, and grinding the dried Pongamia glabrous seeds to obtain Pongamia glabrous seeds powder; the oven is preheated to 80° C.; (2) Extraction: Place the Pongamia pubescens seed powder in an extraction bottle, add a mixed solvent at a solid-liquid ratio of 1:10, and extract at an ultrasonic power of 200 W, an ultrasonic time of 30 min, and an ultrasonic temperature of 60° C. After extraction, filter to obtain a filtrate and a filter residue respectively; wherein the mixed solvent is a mixed solvent of ethanol and n-propanol at a volume ratio of 2:3; (3) mixing the filter residue and the culture medium together and culturing them at 27±1° C. for 7 days, and mixing the obtained culture solution with the filtrate in step (2), wherein the bacterial species is Bacillus subtilis and the inoculation amount is 6%; The composition of the culture medium is as follows: glucose 10 g / L, inositol 0.01 g / L, riboflavin 3.5 g / L, vitamin B12 4.2 mg / L, (NH4)2SO4 1 g / L, MgSO4·7H2O 0.2 g / L, NaCl 0.3 / L, K2HPO4 1 g / L, and the balance is water; natural pH; (4) adding an equal volume of deionized water to the mixed solution obtained in step (3) and letting it stand, taking the upper layer of liquid, and then rotary evaporating it until it becomes a paste; The preparation method of the composition comprises: S01: dissolving Pongamia glabrous seed oil, Silybum marianum seed oil and Nannochloropsis oculata extract in ethanol to obtain a mixture 1; S02: mixing the remaining raw materials with the lower layer liquid in step (4) to obtain mixture 2; S03: Apply mixture 1 to the inner wall of the container, dry it, add mixture 2, and then rotate and shake at 35°C to prepare the product.

2. The biological sunscreen and barrier repair additive composition according to claim 1, characterized in that: The composition is added to daily chemicals at a concentration of 0.1-5 wt %.

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