A fully automatic chlorophyll A determination device

Abstract

The present invention relates to the technical field of chlorophyll A determination equipment, and in particular to a fully automatic chlorophyll A determination device, wherein an automatic steel ball adding device, a sample tray, an automatic head changing and liquid transfer device, a rocker arm oscillation device, an ice bath device, a water removal fan and a centrifuge are sequentially arranged in the middle of the whole machine housing from left to right and from front to back, a drawer is arranged below the automatic head changing and liquid transfer device, a three-coordinate moving mechanism is installed on the upper part of the whole machine housing, and a Z-axis clamp is installed on the Z-axis of the three-coordinate moving mechanism. The beneficial effect of the present invention is that the device of the present invention can automatically carry out the entire determination process of chlorophyll A, greatly improving the work efficiency.

Description

Technical Field

[0001] The present invention relates to the technical field of chlorophyll A determination equipment, and in particular to a fully automatic chlorophyll A determination device. Background Art

[0002] Chlorophyll is an important photosynthetic pigment in plant photosynthesis. Chlorophyll A is an important indicator for estimating phytoplankton biomass. By measuring the content of chlorophyll A in phytoplankton in water, the primary productivity and eutrophication level of the water body can be understood. In environmental monitoring, the chlorophyll A content is one of the indicators for evaluating the eutrophication of water bodies. The massive growth of phytoplankton is the main phenomenon of eutrophication of water bodies. Chlorophyll A is a type of chlorophyll contained in all phytoplankton categories. It is not only an important indicator for the classification of nutrient status of water bodies, but also can be used to characterize the existing amount of phytoplankton. Therefore, chlorophyll A plays a key role in the evaluation of eutrophication of water bodies.

[0003] To this end, the present application designs a fully automatic chlorophyll A determination device to more efficiently achieve the determination of chlorophyll A. Summary of the invention

[0004] In order to make up for the deficiencies in the prior art, the present invention provides an automatic head-changing liquid transfer device.

[0005] The present invention is achieved through the following technical solutions:

[0006] A fully automatic chlorophyll A determination device, comprising a complete housing, characterized in that:

[0007] An automatic steel ball adding device, a sample tray, an automatic head changing and liquid transfer device, a rocker arm oscillation device, an ice bath device, a water removal fan and a centrifuge are sequentially arranged in the middle of the whole machine housing from left to right and from front to back; a drawer is arranged below the automatic head changing and liquid transfer device; a three-coordinate moving mechanism is installed on the upper part of the whole machine housing; a Z-axis clamp is installed on the Z-axis of the three-coordinate moving mechanism.

[0008] Furthermore, in order to better realize the present invention, the automatic steel ball adding device includes a bracket of a gantry structure, a steel ball feeding device is installed on the bracket, a horizontal slide rail is arranged below the bracket, an electric clamp slides on the horizontal slide rail, the electric clamp clamps the centrifuge tube to pass through the steel ball feeding device, the steel ball feeding device is arranged on one side of the automatic liquid adding position, the electric clamp clamps the centrifuge tube to complete the addition of steel balls and moves to the automatic liquid adding position, a counter is installed on one side of the bracket, the counter is connected to the steel ball feeding device, and steel balls are added when the electric clamp clamps the centrifuge tube and moves to the bottom of the counter.

[0009] Furthermore, in order to better realize the present invention, the steel ball feeding device includes a steel ball cylinder fixedly connected to the bracket, the bottom of the steel ball cylinder is a fixed chassis, the fixed chassis is fixedly connected to the bracket, a driving motor is upright and fixed below the bracket, the motor shaft of the driving motor passes through the fixed chassis to connect to the steel ball turntable, and the steel ball turntable is rotatably connected above the fixed chassis; a plurality of through holes are opened on the outer periphery of the turntable as steel ball temporary storage holes, a through hole is opened on the fixed chassis as a steel ball outlet, a feeding pipe is connected to the bottom of the steel ball outlet, and the feeding pipe is connected to a counter; a stopper is fixedly connected to the inner wall of the steel ball cylinder, the stopper is located directly above the steel ball outlet and close to the steel ball turntable, a plurality of balls are installed on the fixed chassis, and a bearing is provided between the steel ball turntable and the steel ball cylinder.

[0010] Furthermore, in order to better realize the present invention, the automatic head-changing pipetting device includes a pipetting turntable on which a plurality of centrifuge tubes and needle filters are placed, and an electric clamp, a lifting swing arm and a wire drawing module are arranged around the pipetting turntable. The electric clamp cooperates with the Z-axis clamp on the chlorophyll A determination machine to unscrew the tube cover of the centrifuge tube, the lifting swing arm is used to move and extract the sample in the centrifuge tube, the wire drawing module is connected to the head removal mechanism arranged on the lifting swing arm, and is used to replace the needle filter. The bottom of the pipetting turntable is connected to a driving mechanism, and the outer ring of the pipetting turntable is opened with a plurality of The through holes are used as centrifuge tube placement positions, and the inner circle of the pipetting turntable is provided with three circles of through holes as needle filter placement positions. The number of centrifuge tube placement positions is 16, and the needle filter placement positions are 22, 15, and 8 from the outer circle to the inner circle, respectively. A channel that goes straight through the bottom is provided at the center of the pipetting turntable as a head removal and discarding position, and the head removal and discarding position is connected to the drawer below. The lifting swing arm includes an upright lifting and rotating shaft that can be lifted and rotated, and the top of the lifting and rotating shaft is horizontally connected to a liquid collection swing arm, and the liquid collection swing arm is provided with a head removal mechanism connected to a wire drawing module.

[0011] Furthermore, in order to better realize the present invention, the head-removing mechanism includes a head-changing rocker hinged in the middle in the liquid collection swing arm, the rear end of the head-changing rocker is connected to a wire pulling module, the front end of the head-changing rocker contacts the needle filter stuck under the sampling head, and a reset spring is connected between the head-changing rocker and the liquid collection swing arm; the wire pulling module includes a wire pulling wire fixedly connected to the rear end of the head-changing rocker, and the wire pulling wire is connected to a wire pulling motor arranged on one side of the pipetting turntable.

[0012] Furthermore, in order to better realize the present invention, the rocker arm oscillation device includes a horizontal mounting plate, a vertical mounting plate is fixedly connected to one side of the horizontal mounting plate, a rocker arm is hinged on the top of the horizontal mounting plate, a motor is fixedly connected to the bottom of the horizontal mounting plate, a vertical vertical slide rail is installed on the vertical mounting plate, a slider is slidably connected to the vertical slide rail, the motor drives the slider to slide up and down, one end of the rocker arm is hinged to the slider, and the other end is fixedly connected to the test tube rack; a buffer spring is fixedly connected to the upper and lower sides of the vertical mounting plate respectively, the buffer spring is connected to the slider, the motor shaft of the motor is fixedly connected to a cam rocker arm mechanism, the cam rocker arm mechanism drives the slider to move up and down, and a support seat is installed on the horizontal mounting plate, and the support seat is hinged to the middle part of the rocker arm.

[0013] Furthermore, in order to better realize the present invention, a pressure cover is hinged on one side of the upper part of the test tube rack, and the pressure cover is L-shaped. A torsion spring is connected between the pressure cover and the rocker arm. The torsion spring presses the pressure cover tightly against the centrifuge tube in the test tube rack. A push rod is connected to the bottom of the pressure cover; one side of the push rod is adjacent to an electric push rod installed on a horizontal mounting plate, and the electric push rod is connected to a push head. The electric push rod is extended to cause the push head to push the push rod, thereby driving the pressure cover to rotate around the hinged position to release the pressure on the centrifuge tube.

[0014] Based on the above device, the operation process is as follows:

[0015] S1, the circulating water machine starts to reach the set temperature, and the pipeline is filled to clean the pipeline;

[0016] S2, after the centrifuge tube is unscrewed from the bottle cap by the Z-axis gripper, steel balls are added through the automatic steel ball adding device, and then the bottle cap is screwed on and moved to the rocker oscillation device for grinding and oscillation;

[0017] S3, the centrifuge tube after shaking is placed in an ice bath device, and after the ice bath is finished, the tube is dehydrated by a dehydration fan and then sent to a centrifuge to start centrifugation;

[0018] S4, after centrifugation, the samples are sent to the automatic head-changing pipetting device and wait for measurement.

[0019] The beneficial effects of the present invention are:

[0020] The device of the present invention can automatically carry out the entire determination process of chlorophyll A, thereby greatly improving work efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a schematic diagram of the three-dimensional structure of the fully automatic chlorophyll A determination device of the present invention;

[0022] Figure 2 For the present invention Figure 1 A schematic diagram of the three-dimensional structure after removing part of the shell baffle;

[0023] Figure 3 It is a schematic diagram of the three-dimensional structure of the automatic steel ball adding device of the present invention;

[0024] Figure 4 An exploded view of the steel ball feeding device of the present invention;

[0025] Figure 5 It is a schematic diagram of the three-dimensional structure of the automatic head-changing pipetting device of the present invention;

[0026] Figure 6 A top view of the automatic head-changing pipetting device of the present invention;

[0027] Figure 7 It is a three-dimensional structural schematic diagram of the head-removing mechanism of the automatic head-changing pipetting device of the present invention;

[0028] Figure 8 This is a schematic diagram of the three-dimensional structure of the rocker arm oscillating device of the present invention;

[0029] Fig. 9 The second is a schematic diagram of the three-dimensional structure of the rocker arm oscillating device of the present invention;

[0030] Fig.10 It is a schematic diagram of the three-dimensional structure of the rocker arm oscillation device of the present invention after removing the mounting plate.

[0031] In the figure,

[0032] 1. Automatic steel ball adding device, 2. Sample tray, 3. Automatic head changing and liquid transfer device, 4. Rocker oscillation device, 5. Ice bath device, 6. Water removal fan, 7. Centrifuge, 8. Drawer, 9. Three-coordinate moving mechanism, 10. Z-axis clamp, 11. Centrifuge tube,

[0033] 101. Bracket, 102. Steel ball unloading device, 103. Counter, 104. Horizontal slide rail, 105. Electric clamp, 106. Automatic liquid level, 1021. Steel ball cylinder, 1022. Driving motor, 1023. Fixed chassis, 1024. Steel ball turntable, 1025. Stopper, 1026. Steel ball temporary storage hole, 1027. Steel ball outlet, 1028. Unloading pipe,

[0034] 301, liquid transfer turntable, 302, electric gripper, 303, lifting swing arm, 304, wire drawing module, 306, needle filter, 3011, centrifuge tube placement, 3012, needle filter placement, 3031, lifting and rotating shaft, 3032, liquid collection swing arm, 3033, sampling head, 3034, head change rocker, 3041, wire drawing motor, 3042, wire drawing,

[0035] 401. Horizontal mounting plate, 402. Vertical mounting plate, 403. Vertical slide rail, 404. Slider, 405. Rocker arm, 406. Test tube rack, 407. Pressure cover, 408. Electric push rod, 409. Motor, 410. Buffer spring, 4011. Support seat, 4071. Push rod, 4072. Torsion spring, 4081. Push head, 4091. Cam rocker arm mechanism. DETAILED DESCRIPTION

[0036] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Generally, the components of the embodiments of the present invention described and shown in the accompanying drawings here can be arranged and designed in various different configurations.

[0037] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the invention claimed for protection, but merely represents selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without making creative work are within the scope of protection of the present invention.

[0038] Figure 1-Figure 10 A specific embodiment of the present invention is a fully automatic chlorophyll A determination device. Figure 1 Figure 2 As shown, the middle part of the whole machine housing is provided with an automatic steel ball adding device 1, a sample tray 2, an automatic head changing and liquid transfer device 3, a rocker arm oscillation device 4, an ice bath device 5, a water removal fan 6 and a centrifuge 7 in sequence from left to right and from front to back, a drawer 8 is provided below the automatic head changing and liquid transfer device 3, a three-coordinate moving mechanism 9 is installed on the upper part of the whole machine housing, and a Z-axis clamp 10 is installed on the Z-axis of the three-coordinate moving mechanism 9.

[0039] like Figure 3 Figure 4As shown, the automatic steel ball adding device 1 includes a bracket 101 of a gantry structure, a steel ball feeding device 102 is installed on the bracket 101, a horizontal slide rail 104 is arranged below the bracket 101, an electric clamp 105 slides on the horizontal slide rail 104, the electric clamp 1055 clamps the centrifuge tube 11 to pass through the steel ball feeding device 102, the steel ball feeding device 102 is arranged on one side of the automatic liquid adding position 106, the electric clamp 105 clamps the centrifuge tube 11 and moves to the automatic liquid adding position 6 after completing the addition of steel balls, a counter 103 is installed on one side of the bracket 101, the counter 103 is connected to the steel ball feeding device 102, and steel balls are added when the electric clamp 105 clamps the centrifuge tube 11 and moves to the bottom of the counter 103. The steel ball unloading device 1102 includes a steel ball cylinder 1021 fixedly connected to the bracket 101, the bottom of the steel ball cylinder 1021 is a fixed chassis 1023, the fixed chassis 1023 is fixedly connected to the bracket 101, and a driving motor 1022 is vertically fixed below the bracket 101. The motor shaft of the driving motor 1022 passes through the fixed chassis 1023 and is connected to a steel ball turntable 1024. The steel ball turntable 1024 is rotatably connected to the top of the fixed chassis 1023; the outer periphery of the turntable 1024 is provided with a plurality of through holes as The steel ball temporary storage hole 102 has a through hole on the fixed chassis 1023 as a steel ball outlet 1027, the bottom of the steel ball outlet 1027 is connected to a feed pipe 1028, and the feed pipe 1028 is connected to the counter 103; a stopper 1025 is fixedly connected to the inner wall of the steel ball cylinder 1021, and the stopper 1025 is located directly above the steel ball outlet 1027 and close to the steel ball turntable 1024. A number of balls are installed on the fixed chassis 1023, and a bearing is provided between the steel ball turntable 1024 and the steel ball cylinder 1021.

[0040] The method of using the automatic steel ball adding device is as follows: the electric clamp holds the centrifuge tube and moves it to the bottom of the counter. The steel ball tube is filled with steel balls, and the steel balls will fall into the steel ball temporary storage holes of the turntable. The motor drives the turntable to rotate. When the steel ball temporary storage hole is aligned with the steel ball outlet, a steel ball will fall from the feed tube into the centrifuge tube, and the counter counts the steel balls that pass through.

[0041] like Figure 5 Figure 6 Figure 7As shown, the automatic head-changing pipetting device 3 includes a pipetting turntable 301 on which a plurality of centrifuge tubes 11 and a needle filter 306 are placed. An electric clamp 302, a lifting swing arm 303 and a wire drawing module 304 are arranged around the pipetting turntable 301. The electric clamp 302 cooperates with the Z-axis clamp 10 on the chlorophyll A determination machine to unscrew the tube cover of the centrifuge tube 11. The lifting swing arm 303 is used to move and extract the sample in the centrifuge tube 11. The wire drawing module 304 is connected to the head removal mechanism set on the lifting swing arm 303 for replacing the needle filter 306. The bottom of the pipetting turntable 301 is connected to a driving mechanism. The outer ring of the pipetting turntable 301 is provided with a plurality of through holes as centrifuge tubes. Placement position 3011, the inner circle of the pipetting turntable 301 is provided with three circles of several through holes as needle filter placement positions 3012, the number of centrifuge tube placement positions 3011 is 16, the needle filter placement positions 3012 are 22, 15, and 8 from the outer circle to the inner circle, respectively, and a channel directly through the bottom is provided at the center of the pipetting turntable 301 as a head removal and discarding position, which is connected to the drawer 8 below, and the lifting swing arm 303 includes an upright lifting and rotating shaft 3031 which can be raised and lowered and rotated, and the top of the lifting and rotating shaft 3031 is horizontally connected to a liquid collection swing arm 3032, and the liquid collection swing arm 3032 is provided with a head removal mechanism connected to the pull wire module 304. The head-removing mechanism includes a head-changing rocker 3034 hinged in the middle of the liquid collection swing arm 3032, the rear end of the head-changing rocker 3034 is connected to the wire drawing module 304, the front end of the head-changing rocker 3034 abuts against the needle filter 306 stuck under the sampling head 3033, and a reset spring is connected between the head-changing rocker 3034 and the liquid collection swing arm 3032; the wire drawing module 304 includes a wire drawing 3042 fixedly connected to the rear end of the head-changing rocker 3034, and the wire drawing 402 is connected to the wire drawing motor 3041 arranged on one side of the pipetting turntable 301.

[0042] The method of using the automatic head-changing pipetting device is as follows: the turntable rotates, the lifting arm rotates and descends to insert a needle filter, then the lifting arm is lifted, the electric clamp clamps a centrifuge tube, the Z-axis clamp on the whole machine unscrews the tube cover on the current centrifuge tube, and then the lifting arm moves to the top of the centrifuge tube and descends to take the liquid. After the liquid is taken, it moves to the center of the head removal and discarding position, the pull wire motor is activated, and the head-changing rocker is pulled by the pull wire. The head-changing rocker pushes the current needle filter downward to complete the head removal, and the needle filter will fall into the drawer.

[0043] like Figure 8 Fig. 9 Fig.10As shown, the rocker oscillation device 4 includes a horizontal mounting plate 401, a vertical mounting plate 402 is fixedly connected to one side of the horizontal mounting plate 401, a rocker 405 is hinged on the top of the horizontal mounting plate 401, a motor 409 is fixedly connected to the bottom of the horizontal mounting plate 401, a vertical slide rail 403 is installed on the vertical mounting plate 402, a slider 404 is slidably connected to the vertical slide rail 403, the motor 409 drives the slider 404 to slide up and down, one end of the rocker 405 is hinged to the slider 404, and the other end is fixedly connected to the test tube rack 406; a buffer spring 410 is fixedly connected to the upper and lower sides of the vertical mounting plate 402 respectively, the buffer spring 410 is connected to the slider 404, the motor shaft of the motor 409 is fixedly connected to a cam rocker mechanism, the cam rocker mechanism drives the slider 404 to move up and down, and a support seat 4011 is installed on the horizontal mounting plate 401, and the support seat 401 is hinged to the middle part of the rocker 405.

[0044] The operation mode of the rocker oscillation device is as follows: the electric push rod is extended, and the push rod is pushed to make the pressure cover 7 tilt up, and then the centrifuge tube containing the sample and the steel ball can be placed in the test tube rack, the electric push rod is reset, and the pressure cover is also reset and presses the centrifuge tube under the action of the torsion spring, and then the motor is started to drive the slider to move up and down, and drive the rocker to swing back and forth around the hinge point, so that the test tube rack oscillates up and down to break the cell wall.

[0045] The specific usage method of this embodiment is as follows:

[0046] S1, the circulating water machine starts to reach the set temperature, and the pipeline is filled to clean the pipeline;

[0047] S2, after the centrifuge tube 11 is unscrewed from the bottle cap by the Z-axis clamp 10, steel balls are added through the automatic steel ball adding device 1, and then the bottle cap is screwed on and moved to the rocker oscillation device 4 for grinding and oscillation;

[0048] S3, the centrifuge tube 11 after shaking is placed in the ice bath device 5, after the ice bath is finished, it is dehydrated by the dehydration fan 6 and then sent to the centrifuge 7 to start centrifugation;

[0049] S4, after the centrifugation is completed, it is sent to the automatic head-changing pipetting device 3 to wait for measurement in sequence.

[0050] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit it. Other modifications or equivalent substitutions made to the technical solution of the present invention by ordinary technicians in the field should be included in the scope of the claims of the present invention as long as they do not depart from the spirit and scope of the technical solution of the present invention.

Claims

1. A fully automatic chlorophyll A determination device, comprising a complete housing, characterized in that: The middle part of the whole machine housing is provided with an automatic steel ball adding device (1), a sample tray (2), an automatic head changing and liquid transfer device (3), a rocker arm oscillating device (4), an ice bath device (5), a water removal fan (6) and a centrifuge (7) in sequence from left to right and from front to back; a drawer (8) is provided below the automatic head changing and liquid transfer device (3); a three-coordinate moving mechanism (9) is installed on the upper part of the whole machine housing; a Z-axis of the three-coordinate moving mechanism (9) is provided with a Z-axis clamp (10); The automatic head-changing pipetting device (3) comprises a pipetting turntable (301) on which a plurality of centrifuge tubes (11) and needle filters (306) are placed. An electric clamp (302), a lifting swing arm (303) and a wire drawing module (304) are arranged around the pipetting turntable (301). The electric clamp (302) cooperates with a Z-axis clamp (10) on a chlorophyll A determination machine to unscrew the tube cover of the centrifuge tube (11). The lifting swing arm (303) is used to move and extract the sample in the centrifuge tube (11). The wire drawing module (304) is connected to a head removal mechanism arranged on the lifting swing arm (303) and is used to replace the needle filter (306). The bottom of the pipetting turntable (301) is connected to a driving mechanism. The outer ring of the pipetting turntable (301) is provided with a plurality of passages. The holes are used as centrifuge tube placement positions (3011), the inner circle of the liquid transfer turntable (301) is provided with three circles of multiple through holes as needle filter placement positions (3012), the number of the centrifuge tube placement positions (3011) is 16, the number of needle filter placement positions (3012) from the outer circle to the inner circle is 22, 15, and 8, respectively, a channel is provided at the center of the liquid transfer turntable (301) that passes through the bottom as a head removal and discarding position, and the head removal and discarding position is connected to the drawer (8) below, the lifting swing arm (303) includes an upright lifting and rotating shaft (3031) that can be lifted and rotated, the top of the lifting and rotating shaft (3031) is horizontally connected to a liquid collection swing arm (3032), and the liquid collection swing arm (3032) is provided with a head removal mechanism connected to a wire drawing module (304); The head-removing mechanism comprises a head-changing seesaw (3034) hinged in the middle of the liquid-taking swing arm (3032); the rear end of the head-changing seesaw (3034) is connected to the wire drawing module (304); the front end of the head-changing seesaw (3034) abuts against a needle filter (306) stuck under the sampling head (3033); a return spring is connected between the head-changing seesaw (3034) and the liquid-taking swing arm (3032); and the wire drawing module (304) comprises a wire drawing (3042) fixedly connected to the rear end of the head-changing seesaw (3034); the wire drawing module (304) is connected to a wire drawing motor (3041) arranged on one side of the liquid transfer turntable (301).

2. The fully automatic chlorophyll A determination device according to claim 1, characterized in that: The automatic steel ball adding device (1) comprises a support (101) of a gantry structure, a steel ball feeding device (102) is installed on the support (101), a horizontal slide rail (104) is arranged below the support (101), an electric clamp (105) slides on the horizontal slide rail (104), the electric clamp (105) clamps the centrifuge tube (11) and passes through the steel ball feeding device (102), the steel ball feeding device (102) is arranged on one side of an automatic liquid adding position (106), the electric clamp (105) clamps the centrifuge tube (11) and moves to the automatic liquid adding position (106) after completing the addition of steel balls, and a counter (103) is installed on one side of the support (101), the counter (103) is connected to the steel ball feeding device (102), and steel balls are added when the electric clamp (105) clamps the centrifuge tube (11) and moves below the counter (103).

3. The fully automatic chlorophyll A determination device according to claim 2, characterized in that: The steel ball unloading device (102) comprises a steel ball cylinder (1021) fixedly connected to the bracket (101); the bottom of the steel ball cylinder (1021) is a fixed chassis (1023); the fixed chassis (1023) is fixedly connected to the bracket (101); a driving motor (1022) is vertically fixed below the bracket (101); the motor shaft of the driving motor (1022) passes through the fixed chassis (1023) and is connected to a steel ball turntable (1024); the steel ball turntable (1024) is rotatably connected above the fixed chassis (1023); a plurality of through holes are opened on the outer circumference of the steel ball turntable (1024) for rotation. The steel ball temporary storage hole (1026) is provided with a through hole as a steel ball outlet (1027) on the fixed chassis (1023); a feed pipe (1028) is connected to the bottom of the steel ball outlet (1027); the feed pipe (1028) is connected to the counter (103); a stopper (1025) is fixedly connected to the inner wall of the steel ball cylinder (1021); the stopper (1025) is located directly above the steel ball outlet (1027) and abuts against the steel ball turntable (1024); a plurality of balls are installed on the fixed chassis (1023); and a bearing is provided between the steel ball turntable (1024) and the steel ball cylinder (1021).

4. The fully automatic chlorophyll A determination device according to claim 1, characterized in that: The rocker arm oscillation device (4) comprises a horizontal mounting plate (401), a vertical mounting plate (402) fixedly connected to one side of the horizontal mounting plate (401), a rocker arm (405) hingedly connected to the top of the horizontal mounting plate (401), a motor (409) fixedly connected to the bottom of the horizontal mounting plate (401), a vertical slide rail (403) mounted on the vertical mounting plate (402), a slider (404) slidably connected to the vertical slide rail (403), the motor (409) drives the slider (404) to slide up and down, and the rocker arm (405) is hingedly connected to the top of the horizontal mounting plate (401), and a motor (409) is fixedly connected to the bottom of the horizontal mounting plate (401). 405) is hinged to a slider (404) at one end, and is fixedly connected to a test tube rack (406) at the other end; a buffer spring (410) is fixedly connected to the upper and lower sides of the vertical mounting plate (402), respectively, and the buffer spring (410) is connected to the slider (404); the motor shaft of the motor (409) is fixedly connected to a cam rocker mechanism, and the cam rocker mechanism drives the slider (404) to move up and down; a support seat (4011) is installed on the horizontal mounting plate (401), and the support seat (4011) is hinged to the middle part of the rocker arm (405).

5. The fully automatic chlorophyll A determination device according to claim 4, characterized in that: A pressure cover (407) is hingedly connected to one side of the upper side of the test tube rack (406). The pressure cover (407) is L-shaped and is connected to the rocker arm (405) via a torsion spring (4072). The torsion spring (4072) presses the pressure cover (407) against the centrifuge tube in the test tube rack (406). A push rod (4071) is connected to the bottom of the pressure cover (407); One side of the push rod (4071) is adjacent to an electric push rod (408) mounted on the horizontal mounting plate (401), and the electric push rod (408) is connected to a push head (4081). When the electric push rod (408) is extended, the push head (4081) pushes the push rod (4071), thereby driving the pressure cover (407) to rotate around the hinge position, thereby releasing the pressure on the centrifuge tube (11).

6. A method for using the fully automatic chlorophyll A determination device according to claim 1, characterized in that: The following steps are involved: S1, the circulating water machine starts to reach the set temperature, and the pipeline is filled to clean the pipeline; S2, after the centrifuge tube (11) is unscrewed from the bottle cap by the Z-axis clamp (10), steel balls are added through the automatic steel ball adding device (1), and then the bottle cap is screwed on and moved to the rocker oscillation device (4) for grinding and oscillation; S3, the centrifuge tube (11) after shaking is placed in an ice bath device (5), and after the ice bath is finished, it is dehydrated by a dehydration fan (6) and then sent to a centrifuge (7) to start centrifugation; S4, after centrifugation is completed, the samples are sent to the automatic head-changing pipetting device (3) and wait for measurement.

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