A preparation method of skin repair anti-allergic essence

Abstract

A preparation method of skin repair anti-allergic essence, belonging to the field of cosmetic technology, the active ingredients of the essence include liquorice extract, Centella asiatica extract, feverfew extract, peony root extract, baobab pulp extract, palmitoyl hexapeptide 14, tetrahydromethylpyrimidine carboxylic acid, modified DNA sodium and isoprene glycol; wherein, the plant extract is prepared by fermentation enzymolysis method; DNA sodium is embedded in soybean lecithin cholesterol peach gum liposome to prepare nanoliposome, which is easy to slow release absorption and has good stability. The present invention adopts plant extract, modified DNA sodium, palmitoyl hexapeptide 14, tetrahydromethylpyrimidine carboxylic acid and isoprene glycol compound essence, has good skin repair anti-allergic effect, whether it is allergic skin or sensitive skin, can be quickly and efficiently repaired, safe and without side effects, can make the skin restore a good anti-allergic state, form its own barrier, enhance immunity, and prevent allergy recurrence.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and particularly relates to a method for preparing a skin repairing and anti-allergic essence. Background Art

[0002] Wind and sun, large temperature changes, dry environment, contact with pollen, petals, dust, hair, bacteria, spicy or seafood, and inappropriate cosmetics are all causes of skin allergies. Skin allergies have clear allergens, especially during the season change in spring and autumn, skin allergies occur frequently, and skin allergies manifest as congestion, redness, peeling, itching, swelling, papules, etc. It is necessary to isolate the allergens in time and then repair the skin symptoms.

[0003] Sensitive skin refers to thin skin, damaged skin, and no clear allergens. Symptoms include burning, stinging, and tightness, and sometimes accompanied by erythema, capillary dilation, etc. Long-term skin barrier repair is required to bring the skin to a healthy state.

[0004] At present, the existing cosmetics with anti-allergic effects are all based on anti-allergic and skin barrier repair, so as to relieve allergic reactions, strengthen skin condition and enhance skin resistance. However, there are many kinds of cosmetics with different effects. In actual use, there are still problems such as slow anti-allergic repair time and poor effect. Therefore, the product elimination rate is high and the frequency of replacement is fast. Although it is constantly innovating and improving, it still cannot meet the needs of consumers. In the case of severe cases, oral medicine is still needed for anti-allergic treatment, which inevitably has side effects. Summary of the invention

[0005] The existing skin repair and anti-allergic cosmetics have slow effects, unobvious effects, and long treatment courses, which cannot meet the needs of consumers. The present invention provides a method for preparing a skin repair and anti-allergic essence, which uses various plant extracts fermented or enzymatically hydrolyzed by beneficial bacteria, modified DNA sodium after liposome embedding, palmitoyl hexapeptide-14, tetrahydromethylpyrimidine carboxylic acid and isopentyldiol to prepare an essence, which has good skin repair and anti-allergic effects. Whether it is allergic skin or sensitive skin, it can be repaired quickly and efficiently, is safe and has no side effects, can restore the skin to a good anti-allergic state, form its own barrier, enhance immunity, and prevent allergy recurrence. The specific technical scheme is as follows:

[0006] A method for preparing a skin repair and anti-allergic essence comprises the following steps:

[0007] S1, preparation of plant extracts:

[0008] including the preparation of liquorice extract, centella asiatica extract, feverfew extract, peony root extract and baobab pulp extract;

[0009] S2, preparation of modified sodium DNA:

[0010] S2.1: Dissolve soybean lecithin and cholesterol uniformly in anhydrous ethanol at a molar ratio of soybean lecithin: cholesterol = (2-5): 1, and perform rotary evaporation at 55°C-60°C to form a thin film on the container wall to obtain a thin film;

[0011] S2.2: Dissolve peach gum powder in phosphate buffered saline (PBS) at a mass ratio of peach gum powder to sodium DNA = 1: (5-10), then add sodium DNA, mix well, add film, and perform ultrasound at 40KHz-60KHz for 15min-20min in a water bath at 4°C-6°C to obtain an emulsion;

[0012] S2.3: The emulsion is placed in a syringe and repeatedly pushed out through a 0.22 μm to 0.30 μm polycarbonate filter membrane to obtain a nanoemulsion and obtain modified DNA sodium;

[0013] S3, preparing essence:

[0014] S3.1: Prepare, by mass, 1 to 3 parts of liquorice extract, 1 to 3 parts of Centella asiatica extract, 0.5 to 2 parts of feverfew extract, 0.5 to 2 parts of peony root extract, 2 to 5 parts of baobab pulp extract, 0.5 to 1 part of palmitoyl hexapeptide-14, 0.5 to 3 parts of tetrahydromethylpyrimidine carboxylic acid, 0.2 to 3 parts of modified DNA sodium, 0.5 to 3 parts of isopentyl glycol and 30 to 50 parts of purified water;

[0015] S3.2: Heat purified water to 50°C-60°C according to mass proportions, add tetrahydromethylpyrimidine carboxylic acid, modified sodium DNA and isoprene glycol and mix evenly, then cool to 38°C-45°C, add licorice extract, Centella asiatica extract, feverfew extract, peony root extract, baobab pulp extract and palmitoyl hexapeptide-14, mix evenly to obtain an essence.

[0016] In S2.2 of the above preparation method, the amount of the phosphate buffer solution (PBS) is 80 to 100 times the mass of the peach gum powder; and the amount of the film added is 3% to 5% of the mass of the DNA sodium.

[0017] In S1 of the above preparation method, the preparation method of the licorice extract is:

[0018] The licorice is crushed, anhydrous ethanol is added for soaking, a sieve is filtered to obtain a sieve liquid, and the sieve liquid is concentrated and dried to obtain a licorice crude extract; the licorice crude extract is suspended in a pH 7.0-pH 7.2 glucose aqueous solution, Bacillus subtilis and serine protease are added, and the mixture is cultured at 36-38° C. for 20-25 hours to obtain a fermentation hydrolyzate, and the mixture is sterilized and enzyme-killed in a hot water bath, and after cooling to room temperature, the fermentation hydrolyzate is filtered using a 3 kDa ultrafiltration membrane, and the filtrate with a diameter less than 3 kDa is concentrated and dried to obtain a licorice extract;

[0019] The amount of anhydrous ethanol added is 3 to 6 times the mass of licorice; the soaking time of anhydrous ethanol is 30 to 60 minutes; the mesh size of the sieve is 325 meshes; the amount of pH7.0 to pH7.2 glucose aqueous solution is 5 to 8 times the mass of the crude extract of licorice, and the glucose content of the glucose aqueous solution is 2 g / L to 3 g / L; the amount of Bacillus subtilis added is 1% to 1.5% of the mass of the crude extract of licorice; the amount of serine protease added is 0.2% to 0.5% of the mass of the crude extract of licorice; the temperature of the hot water bath sterilization and enzyme inactivation is 90°C to 100°C, and the time is 15 to 20 minutes.

[0020] In S1 of the above preparation method, the preparation method of the Centella asiatica extract is:

[0021] The method comprises the following steps: grinding Centella asiatica, adding 6 to 8 times the mass of water, adjusting the pH value to 5.0 to 5.5 with hydrochloric acid, adding hemicellulase, performing enzymolysis at 50 to 60° C. for 1.5 to 2 hours, inactivating the enzyme by hot water bath, cooling to room temperature, filtering by using a 5 kDa ultrafiltration membrane, concentrating and drying the filtrate with a mass less than 5 kDa, and obtaining a crude extract of Centella asiatica; suspending the crude extract of Centella asiatica in 6 to 8 times the mass of water, then adding aspartic acid protease and cysteine ​​protease, performing enzymolysis at 37 to 38° C. for 1.5 to 2 hours, inactivating the enzyme by hot water bath, cooling to room temperature, filtering by using a 3 kDa ultrafiltration membrane, concentrating and drying the filtrate with a mass less than 3 kDa, and obtaining a Centella asiatica extract;

[0022] The amount of hemicellulase added is 1% to 2% of the mass of Centella asiatica; the amount of aspartic protease added is 0.5% to 1% of the mass of the crude extract of Centella asiatica; the amount of cysteine ​​protease added is 0.5% to 1% of the mass of the crude extract of Centella asiatica; the temperature of the hot water bath enzyme inactivation is 90°C to 100°C, and the time is 15min to 20min.

[0023] In S1 of the above preparation method, the preparation method of the feverfew extract is:

[0024] Take feverfew and grind it, add 5 to 6 times the mass of water, soak it in hot water at 50 to 60 degrees Celsius for 60 to 90 minutes, cool it to room temperature, add anhydrous ethanol 1 to 3 times the mass of feverfew, continue soaking for 30 to 50 minutes, filter it with a 5kDa ultrafiltration membrane, take the filtrate with a mass less than 5kDa, concentrate and dry it, and obtain the feverfew extract.

[0025] In S1 of the above preparation method, the preparation method of the peony root extract is:

[0026] Wash and crush the peony root, add 6 to 8 times the mass of water, adjust the pH to 6.0 to 6.5, add hemicellulase and papain, perform enzymolysis at 40 to 45° C. for 1.5 to 2 hours, inactivate the enzyme in a hot water bath, cool to room temperature, filter using a 3 kDa ultrafiltration membrane, concentrate and dry the filtrate with less than 3 kDa, and obtain a peony root crude extract; suspend the peony root crude extract in an ethanol aqueous solution, mix and soak, filter using a 3 kDa ultrafiltration membrane, concentrate and dry the filtrate with less than 3 kDa, and obtain a peony root extract;

[0027] The amount of hemicellulase added is 2% to 3% of the mass of the peony root; the amount of papain added is 1% to 1.5% of the mass of the peony root; the temperature of the hot water bath to inactivate the enzyme is 90° C. to 100° C., and the time is 15 min to 20 min; the volume concentration of ethanol in the ethanol aqueous solution is 20% to 30%; and the time of the mixed soaking is 20 min to 30 min.

[0028] In S1 of the above preparation method, the preparation method of the baobab pulp extract is:

[0029] Baobab pulp is taken, crushed, added to water of 6 to 8 times its mass, then bifidobacteria are added, fermented at 37 to 42°C for 12 to 14 hours, sterilized in a boiling water bath, and cooled to room temperature to obtain a fermentation liquid; anhydrous ethanol is added to the fermentation liquid for mixing and soaking, filtered using a 3 kDa ultrafiltration membrane, and the filtrate less than 3 kDa is concentrated and dried to obtain a baobab pulp extract;

[0030] The amount of bifidobacteria added is 1% to 3% of the mass of baobab pulp; the temperature of the boiling water bath sterilization is 90° C. to 100° C., and the time is 15 min to 20 min; the amount of anhydrous ethanol added is 2 to 3 times the mass of baobab pulp; and the time of mixing and soaking in anhydrous ethanol is 20 min to 30 min.

[0031] Compared with the prior art, the preparation method of the skin repairing and anti-allergic essence of the present invention has the following beneficial effects:

[0032] 1. The method of the present invention is designed to modify the DNA sodium, and the DNA sodium is embedded in soybean lecithin-cholesterol-peach gum liposomes to prepare nanoliposome inclusion compounds, and the embedding steps and parameters are designed. The method can embed the DNA sodium well, improve the mixing and storage stability of the DNA sodium, and maintain the use effectiveness of the DNA sodium to the maximum extent. It has good fusion with other ingredients, and is not prone to embedding failure during subsequent compounding. The liposomes added with peach gum also have good sustained release properties for the DNA sodium, prolong the release and absorption time, will not interfere with the absorption of other ingredients, will not cause skin overnutrition, and absorption disorder; after the DNA sodium is gradually absorbed by the skin, it can promote the cells to restore a healthy state and promote cell division and proliferation, make the cortex thicker, form a natural water-retaining barrier, and repair the dryness, wrinkles and roughness of the skin well.

[0033] Second, the method of the present invention is to add licorice extract to the essence, licorice is soaked and filtered with anhydrous ethanol to obtain some small molecules and alcohol-soluble substances, and fermented and hydrolyzed with Bacillus subtilis and serine protease to obtain small molecules below 3kDa, including a variety of alcohol-soluble and water-soluble active substances and small molecule peptides and other fermentation and hydrolysis products. Compared with the simple ethanol solvent extract, the licorice extract is richer in active substances, can improve the skin repair, anti-allergy and anti-inflammatory effects, has a proliferative effect on cells, promotes the proliferation of vascular endothelial cells, and promotes the production of collagen-4 type, which greatly improves the activity of the skin. In addition, after fermentation and hydrolysis, part of the licorice odor can be removed.

[0034] 3. Centella asiatica extract has the effect of removing scars, improving the activity of fibroblasts, promoting the recovery of acne marks and scars, and promoting cells to synthesize collagen and extracellular matrix, and has the effects of whitening, removing spots, sterilization, and anti-inflammatory. The method of the present invention is designed to add Centella asiatica extract to the essence, and the Centella asiatica is enzymatically hydrolyzed with hemicellulase, and then enzymatically hydrolyzed with aspartic acid protease and cysteine ​​protease to obtain fiber enzymatic hydrolysis products and protein enzymatic hydrolysis products, complex vitamins and small molecule polypeptides, which can further improve the efficacy of Centella asiatica extract and have good repair and anti-allergic effects.

[0035] 4. The method of the present invention is designed to add feverfew extract to the essence. The feverfew extract has anti-inflammatory and antioxidant effects, inhibits induced skin allergies, can enhance the resistance of capillaries, inhibit capillary permeability and has anti-inflammatory effects.

[0036] 5. The method of the present invention is designed to add peony root extract to the essence, and the water and ethanol extracts obtained after enzymatic hydrolysis of the peony root with hemicellulase and papain can enrich the ingredients of the extract and improve the repair effect on the skin's protective barrier.

[0037] 6. The method of the present invention is designed to add baobab pulp extract to the essence, and the baobab pulp is fermented with bifidobacteria to obtain water-soluble and alcohol-soluble fermentation products, as well as fruit oil substances, which have excellent anti-inflammatory, antioxidant and moisturizing effects. The fermentation product can also promote the repair of the skin barrier, improve immunity, and restore the body's anti-allergic properties.

[0038] 7. The method of the present invention designs the addition of palmitoyl hexapeptide-14 to the essence. Palmitoyl hexapeptide-14 can stimulate the production of collagen and the proliferation of fibroblasts. When used in combination with other plant extract ingredients and DNA sodium ingredients, it can thicken the cortex, repair sensitive skin, restore skin vitality, and reduce non-allergic and allergen damage to the skin.

[0039] 8. The method of the present invention designs the addition of ectoine, i.e., ectoine, into the essence, which has moisturizing and skin conditioning effects.

[0040] 9. The method of the present invention designs the addition of isopentyl glycol to the essence, which has the effect of removing odor, antibacterial and antiseptic properties, and also has the effects of emollient and mild moisturizing, and can improve the skin feel of the cosmetic formula.

[0041] In summary, the plant extract prepared by the fermentation or enzymatic hydrolysis method of the present invention has a good hyaluronidase inhibition effect. Compared with the plant extract extracted by ethanol, the hyaluronidase inhibition rate is greatly improved; the plant extract contains rich small molecule polypeptides and other beneficial fermentation or enzymatic hydrolysis products, has good cell repair effects, improves the natural barrier properties of cells, enhances immunity, and achieves an anti-allergic effect.

[0042] The modified DNA sodium has a better moisturizing effect and can be absorbed slowly. The modified DNA sodium is more stable, highly effective, and easy to absorb, which can promote cells to return to a healthy state and promote cell division and proliferation, thicken the cortex, form a natural water-retaining barrier, and effectively repair dryness, wrinkles, and roughness of the skin.

[0043] The combination of various plant extracts and DNA sodium can significantly increase the speed of repair. Compared with plant extracts extracted by ethanol, substances extracted by fermentation or enzymatic hydrolysis have a faster repair and acne removal effect, shorten the course of treatment, and have obvious effects; and after 30 days of discontinuation, the skin can restore a good anti-allergic state, form its own barrier, enhance immunity, and prevent the recurrence of allergies. DETAILED DESCRIPTION

[0044] The present invention is further described below in conjunction with specific implementation cases, but the present invention is not limited to these embodiments.

[0045] Example 1

[0046] A method for preparing a skin repair and anti-allergic essence comprises the following steps:

[0047] S1, preparation of plant extracts:

[0048] S1.1, preparation of licorice extract:

[0049] The licorice is crushed, anhydrous ethanol of 5 times the mass of the licorice is added, and soaked for 40 minutes, filtered through a 325-mesh screen to obtain a sieve liquid, concentrated and dried to obtain a licorice crude extract; the licorice crude extract is suspended in a 6-times mass pH 7.1 glucose aqueous solution, the glucose content of the glucose aqueous solution is 2.5 g / L, 1.2% mass of Bacillus subtilis of the licorice crude extract and 0.4% mass of serine protease of the licorice crude extract are added, and the mixture is cultured at 37°C for 22 hours to obtain a fermentation hydrolyzate, sterilized and inactivated in a hot water bath at 95°C for 15 minutes, cooled to room temperature, and the fermentation hydrolyzate is filtered through a 3 kDa ultrafiltration membrane, and the filtrate less than 3 kDa is concentrated and dried to obtain a licorice extract.

[0050] S1.2, preparation of Centella asiatica extract:

[0051] The method comprises the following steps: grinding Centella asiatica, adding 7 times the mass of water, adjusting the pH value to 5.4 with hydrochloric acid, adding 1.5% by mass of hemicellulase of Centella asiatica, performing enzymolysis at 55°C for 1.5 hours, inactivating the enzyme in a hot water bath, cooling to room temperature, filtering with a 5 kDa ultrafiltration membrane, concentrating and drying the filtrate with a mass less than 5 kDa, and obtaining a crude extract of Centella asiatica; suspending the crude extract of Centella asiatica in 7 times the mass of water, and then adding 0.6% by mass of aspartic acid protease of the crude extract of Centella asiatica and 0.8% by mass of cysteine ​​protease of the crude extract of Centella asiatica, performing enzymolysis at 37.5°C for 2 hours, inactivating the enzyme in a hot water bath at 95°C for 20 minutes, cooling to room temperature, filtering with a 3 kDa ultrafiltration membrane, concentrating and drying the filtrate with a mass less than 3 kDa, and obtaining a Centella asiatica extract.

[0052] S1.3, preparation of feverfew extract:

[0053] Crush the feverfew, add 5.5 times the mass of water, soak in 55°C hot water for 70 minutes, cool to room temperature, add anhydrous ethanol twice the mass of the feverfew, continue soaking for 40 minutes, filter with a 5kDa ultrafiltration membrane, take the filtrate less than 5kDa, concentrate and dry, and obtain the feverfew extract.

[0054] S1.4, preparation of peony root extract:

[0055] Wash and crush the peony root, add 7 times the weight of water, adjust the pH to 6.3, add 2.5% hemicellulase and 1.2% papain of the peony root, perform enzymolysis at 42°C for 2h, inactivate the enzyme in a hot water bath at 95°C for 15min, cool to room temperature, filter with a 3kDa ultrafiltration membrane, concentrate and dry the filtrate with less than 3kDa, and obtain a peony root crude extract; suspend the peony root crude extract in an ethanol aqueous solution with a volume concentration of 25%, mix and soak for 25min, filter with a 3kDa ultrafiltration membrane, concentrate and dry the filtrate with less than 3kDa, and obtain a peony root extract;

[0056] S1.5, Preparation of Baobab Fruit Extract:

[0057] In S1 of the above preparation method, the preparation method of the baobab pulp extract is:

[0058] Baobab pulp is taken, crushed, added to water of 7 times its mass, then bifidobacteria of 2% of the mass of the baobab pulp are added, fermented at 40°C for 13 hours, sterilized in a boiling water bath at 95°C for 20 minutes, and cooled to room temperature to obtain a fermentation liquid; anhydrous ethanol of 2.5 times the mass of the baobab pulp is added to the fermentation liquid, mixed and soaked for 25 minutes, filtered with a 3kDa ultrafiltration membrane, and the filtrate less than 3kDa is concentrated and dried to obtain a baobab pulp extract;

[0059] S2, preparation of modified sodium DNA:

[0060] S2.1: Dissolve soybean lecithin and cholesterol uniformly in anhydrous ethanol at a molar ratio of soybean lecithin to cholesterol = 3:1, and perform rotary evaporation at 58° C. to form a thin film on the container wall to obtain a thin film;

[0061] S2.2: Peach gum powder was dissolved in phosphate buffered saline (PBS) with a mass ratio of peach gum powder to sodium DNA = 1:8, and then sodium DNA was added. After mixing evenly, a 4% sodium DNA film was added, and ultrasound was performed at 50KHz for 15 minutes in a 5°C water bath to obtain an emulsion;

[0062] S2.3: The emulsion is placed in a syringe and repeatedly pushed out through a 0.26 μm polycarbonate filter to obtain a nanoemulsion and obtain modified DNA sodium;

[0063] S3, preparing essence:

[0064] S3.1: Prepare, by mass, 2 parts of liquorice extract, 2 parts of Centella asiatica extract, 1 part of feverfew extract, 1 part of peony root extract, 3 parts of baobab pulp extract, 0.8 parts of palmitoyl hexapeptide-14, 2 parts of tetrahydromethylpyrimidine carboxylic acid, 3 parts of modified DNA sodium, 2 parts of isopentyl glycol and 40 parts of purified water;

[0065] S3.2: Heat purified water to 55°C according to mass proportions, add tetrahydromethylpyrimidine carboxylic acid, modified sodium DNA and isoprene glycol and mix evenly, then cool to 40°C, add licorice extract, Centella asiatica extract, feverfew extract, peony root extract, baobab pulp extract and palmitoyl hexapeptide-14, mix evenly to obtain an essence.

[0066] Example 2

[0067] A method for preparing a skin repair and anti-allergic essence comprises the following steps:

[0068] S1, preparation of plant extracts:

[0069] S1.1, preparation of licorice extract:

[0070] The licorice is crushed, anhydrous ethanol is added in an amount of 3 times the mass of the licorice to soak for 60 minutes, a 325-mesh screen is used to filter, a sieve liquid is obtained, and the sieve liquid is concentrated and dried to obtain a licorice crude extract; the licorice crude extract is suspended in a 5-times mass of a pH 7.2 glucose aqueous solution, the glucose content of the glucose aqueous solution is 2 g / L, 1.5% of the mass of the licorice crude extract of Bacillus subtilis and 0.2% of the mass of the licorice crude extract of serine protease are added, and the licorice crude extract is cultured at 38°C for 20 hours to obtain a fermentation hydrolyzate, and the fermentation hydrolyzate is sterilized and enzyme-killed in a hot water bath at 100°C for 15 minutes, and after cooling to room temperature, the fermentation hydrolyzate is filtered through a 3 kDa ultrafiltration membrane, and a filtrate less than 3 kDa is concentrated and dried to obtain a licorice extract.

[0071] S1.2, preparation of Centella asiatica extract:

[0072] The method comprises the following steps: grinding Centella asiatica, adding 8 times the mass of water, adjusting the pH value to 5.0 with hydrochloric acid, adding 2% of the mass of hemicellulase of Centella asiatica, performing enzymolysis at 50°C for 2 hours, inactivating the enzyme in a hot water bath, cooling to room temperature, filtering with a 5kDa ultrafiltration membrane, concentrating and drying the filtrate with a mass less than 5kDa, and obtaining a crude extract of Centella asiatica; suspending the crude extract of Centella asiatica in 6 times the mass of water, and then adding 1% of the mass of aspartic protease of the crude extract of Centella asiatica and 0.5% of the mass of cysteine ​​protease of the crude extract of Centella asiatica, performing enzymolysis at 38°C for 1.5 hours, inactivating the enzyme in a hot water bath at 100°C for 15 minutes, cooling to room temperature, filtering with a 3kDa ultrafiltration membrane, concentrating and drying the filtrate with a mass less than 3kDa, and obtaining a Centella asiatica extract.

[0073] S1.3, preparation of feverfew extract:

[0074] Crush feverfew, add 6 times the mass of water, soak in 50°C hot water for 90 minutes, cool to room temperature, add 1 times the mass of anhydrous ethanol of feverfew, continue soaking for 50 minutes, filter with a 5kDa ultrafiltration membrane, take the filtrate less than 5kDa, concentrate and dry, and obtain the feverfew extract.

[0075] S1.4, preparation of peony root extract:

[0076] Wash and crush the peony root, add 6 times the weight of water, adjust the pH to 6.5, add 2% hemicellulase and 1.5% papain of the peony root, perform enzymolysis at 40°C for 2h, inactivate the enzyme in a hot water bath at 90°C for 20min, cool to room temperature, filter with a 3kDa ultrafiltration membrane, concentrate and dry the filtrate with less than 3kDa, and obtain a peony root crude extract; suspend the peony root crude extract in an ethanol aqueous solution with a volume concentration of 20%, mix and soak for 30min, filter with a 3kDa ultrafiltration membrane, concentrate and dry the filtrate with less than 3kDa, and obtain a peony root extract;

[0077] S1.5, Preparation of Baobab Fruit Extract:

[0078] In S1 of the above preparation method, the preparation method of the baobab pulp extract is:

[0079] Baobab pulp is taken, crushed, added to water of 6 times its mass, then 3% bifidobacterium of the baobab pulp is added, fermented at 37°C for 14 hours, sterilized in a boiling water bath at 90°C for 20 minutes, and cooled to room temperature to obtain a fermentation liquid; anhydrous ethanol of 2 times the mass of the baobab pulp is added to the fermentation liquid, mixed and soaked for 30 minutes, filtered with a 3kDa ultrafiltration membrane, and the filtrate less than 3kDa is concentrated and dried to obtain a baobab pulp extract;

[0080] S2, preparation of modified sodium DNA:

[0081] S2.1: Dissolve soybean lecithin and cholesterol uniformly in anhydrous ethanol at a molar ratio of soybean lecithin to cholesterol = 2:1, and perform rotary evaporation at 60°C to form a thin film on the container wall to obtain a thin film;

[0082] S2.2: Peach gum powder was dissolved in phosphate buffered saline (PBS) with a mass ratio of peach gum powder to sodium DNA = 1:5, and then sodium DNA was added. After mixing evenly, a 3% sodium DNA film was added, and ultrasound was performed at 40KHz in a 6°C water bath for 20 min to obtain an emulsion;

[0083] S2.3: The emulsion is placed in a syringe and repeatedly pushed out through a 0.22 μm polycarbonate filter to obtain a nanoemulsion and obtain modified DNA sodium;

[0084] S3, preparing essence:

[0085] S3.1: Prepare, by mass, 3 parts of liquorice extract, 1 part of Centella asiatica extract, 2 parts of feverfew extract, 0.5 parts of peony root extract, 5 parts of baobab pulp extract, 0.5 parts of palmitoyl hexapeptide-14, 3 parts of tetrahydromethylpyrimidine carboxylic acid, 2 parts of modified sodium DNA, 3 parts of isopentyl glycol and 30 parts of purified water;

[0086] S3.2: Heat purified water to 60°C according to mass proportions, add tetrahydromethylpyrimidine carboxylic acid, modified sodium DNA and isoprene glycol and mix evenly, then cool to 38°C, add licorice extract, Centella asiatica extract, feverfew extract, peony root extract, baobab pulp extract and palmitoyl hexapeptide-14, mix evenly to obtain an essence.

[0087] Example 3

[0088] A method for preparing a skin repair and anti-allergic essence comprises the following steps:

[0089] S1, preparation of plant extracts:

[0090] S1.1, preparation of licorice extract:

[0091] The licorice is crushed, anhydrous ethanol of 6 times the mass of the licorice is added, and the ethanol is soaked for 60 minutes, and a 325-mesh screen is used for filtering to obtain a sieve liquid, and the sieve liquid is concentrated and dried to obtain a licorice crude extract; the licorice crude extract is suspended in an 8-times-mass pH 7.2 glucose aqueous solution, wherein the glucose content of the glucose aqueous solution is 3 g / L, 1.5% of the mass of the licorice crude extract of Bacillus subtilis and 0.5% of the mass of the licorice crude extract of serine protease are added, and the licorice crude extract is cultured at 38° C. for 25 hours to obtain a fermentation hydrolyzate, and the fermentation hydrolyzate is sterilized and inactivated in a hot water bath at 100° C. for 20 minutes, and after cooling to room temperature, the fermentation hydrolyzate is filtered through a 3 kDa ultrafiltration membrane, and the filtrate less than 3 kDa is concentrated and dried to obtain a licorice extract.

[0092] S1.2, preparation of Centella asiatica extract:

[0093] The method comprises the following steps: grinding Centella asiatica, adding 8 times the mass of water, adjusting the pH value to 5.5 with hydrochloric acid, adding 2% of the mass of hemicellulase of Centella asiatica, performing enzymolysis at 60°C for 2 hours, inactivating the enzyme in a hot water bath, cooling to room temperature, filtering with a 5kDa ultrafiltration membrane, concentrating and drying the filtrate with a mass less than 5kDa, and obtaining a crude Centella asiatica extract; suspending the crude Centella asiatica extract in 8 times the mass of water, and then adding 1% of the mass of aspartic acid protease and 1% of the mass of cysteine ​​protease of the crude Centella asiatica extract, performing enzymolysis at 38°C for 2 hours, inactivating the enzyme in a hot water bath at 100°C for 20 minutes, cooling to room temperature, filtering with a 3kDa ultrafiltration membrane, concentrating and drying the filtrate with a mass less than 3kDa, and obtaining a Centella asiatica extract.

[0094] S1.3, preparation of feverfew extract:

[0095] Crush the feverfew, add 6 times the mass of water, soak in 60°C hot water for 90 minutes, cool to room temperature, add anhydrous ethanol 3 times the mass of the feverfew, continue soaking for 50 minutes, filter with a 5kDa ultrafiltration membrane, take the filtrate less than 5kDa, concentrate and dry, and obtain the feverfew extract.

[0096] S1.4, preparation of peony root extract:

[0097] Wash and crush the peony root, add 8 times the weight of water, adjust the pH to 6.5, add 3% hemicellulase and 1.5% papain of the peony root, perform enzymolysis at 45°C for 2h, inactivate the enzyme in a hot water bath at 100°C for 20min, cool to room temperature, filter with a 3kDa ultrafiltration membrane, concentrate and dry the filtrate with less than 3kDa, and obtain a peony root crude extract; suspend the peony root crude extract in an ethanol aqueous solution with a volume concentration of 30%, mix and soak for 30min, filter with a 3kDa ultrafiltration membrane, concentrate and dry the filtrate with less than 3kDa, and obtain a peony root extract;

[0098] S1.5, Preparation of Baobab Fruit Extract:

[0099] In S1 of the above preparation method, the preparation method of the baobab pulp extract is:

[0100] Baobab pulp is taken, crushed, added to water with an amount 8 times the mass, then bifidobacteria with an amount 3% by mass of the baobab pulp is added, fermented at 42° C. for 14 hours, sterilized in a boiling water bath at 100° C. for 20 minutes, and cooled to room temperature to obtain a fermentation liquid; anhydrous ethanol with an amount 3 times the mass of the baobab pulp is added to the fermentation liquid, mixed and soaked for 30 minutes, filtered using a 3 kDa ultrafiltration membrane, and the filtrate with an amount less than 3 kDa is concentrated and dried to obtain a baobab pulp extract;

[0101] S2, preparation of modified sodium DNA:

[0102] S2.1: Dissolve soybean lecithin and cholesterol uniformly in anhydrous ethanol at a molar ratio of soybean lecithin to cholesterol = 5:1, and perform rotary evaporation at 60°C to form a thin film on the container wall to obtain a thin film;

[0103] S2.2: Peach gum powder was dissolved in phosphate buffered saline (PBS) with a mass ratio of peach gum powder to sodium DNA = 1:10, and then sodium DNA was added. After mixing evenly, a 5% sodium DNA film was added, and ultrasound was performed at 60 kHz in a 6°C water bath for 20 min to obtain an emulsion;

[0104] S2.3: The emulsion is placed in a syringe and repeatedly pushed out through a 0.30 μm polycarbonate filter to obtain a nanoemulsion and obtain modified DNA sodium;

[0105] S3, preparing essence:

[0106] S3.1: Prepare, by mass, 3 parts of liquorice extract, 3 parts of Centella asiatica extract, 2 parts of feverfew extract, 2 parts of peony root extract, 5 parts of baobab pulp extract, 1 part of palmitoyl hexapeptide-14, 3 parts of tetrahydromethylpyrimidine carboxylic acid, 0.2 parts of modified DNA sodium, 3 parts of isopentyl glycol and 50 parts of purified water;

[0107] S3.2: Heat purified water to 60°C according to mass proportions, add tetrahydromethylpyrimidine carboxylic acid, modified sodium DNA and isoprene glycol and mix evenly, then cool to 45°C, add licorice extract, Centella asiatica extract, feverfew extract, peony root extract, baobab fruit extract and palmitoyl hexapeptide-14, mix evenly to obtain an essence.

[0108] Example 4

[0109] A method for preparing a skin repair and anti-allergic essence comprises the following steps:

[0110] S1, preparation of plant extracts:

[0111] S1.1, preparation of licorice extract:

[0112] The licorice is crushed, anhydrous ethanol of 6 times the mass of the licorice is added, and soaked for 30 minutes, filtered through a 325-mesh screen to obtain a sieve liquid, concentrated and dried to obtain a licorice crude extract; the licorice crude extract is suspended in a pH 7.0 glucose aqueous solution of 8 times the mass, the glucose content of the glucose aqueous solution is 3g / L, 1% of the mass of the licorice crude extract of Bacillus subtilis and 0.5% of the mass of the licorice crude extract of serine protease are added, and the mixture is cultured at 36°C for 25 hours to obtain a fermentation hydrolyzate, sterilized and enzyme-killed in a hot water bath at 90°C for 20 minutes, cooled to room temperature, and the fermentation hydrolyzate is filtered through a 3kDa ultrafiltration membrane, and the filtrate less than 3kDa is concentrated and dried to obtain a licorice extract.

[0113] S1.2, preparation of Centella asiatica extract:

[0114] The method comprises the following steps: grinding Centella asiatica, adding 6 times the mass of water, adjusting the pH value to 5.5 with hydrochloric acid, adding 1% hemicellulase by mass of Centella asiatica, performing enzymolysis at 60°C for 1.5 hours, inactivating the enzyme by hot water bath, cooling to room temperature, filtering with a 5kDa ultrafiltration membrane, concentrating and drying the filtrate with a mass less than 5kDa, and obtaining a crude Centella asiatica extract; suspending the crude Centella asiatica extract in 8 times the mass of water, and then adding 0.5% aspartic acid protease by mass of the crude Centella asiatica extract and 1% cysteine ​​protease by mass of the crude Centella asiatica extract, performing enzymolysis at 37°C for 2 hours, inactivating the enzyme by hot water bath at 90°C for 20 minutes, cooling to room temperature, filtering with a 3kDa ultrafiltration membrane, concentrating and drying the filtrate with a mass less than 3kDa, and obtaining a Centella asiatica extract.

[0115] S1.3, preparation of feverfew extract:

[0116] Crush the feverfew, add 5 times the mass of water, soak in 60°C hot water for 60 minutes, cool to room temperature, add anhydrous ethanol 3 times the mass of the feverfew, continue soaking for 30 minutes, filter with a 5kDa ultrafiltration membrane, take the filtrate less than 5kDa, concentrate and dry, and obtain the feverfew extract.

[0117] S1.4, preparation of peony root extract:

[0118] Wash and crush the peony root, add 8 times the weight of water, adjust the pH to 6.0, add 3% hemicellulase and 1% papain of the peony root, perform enzymolysis at 45°C for 1.5 hours, inactivate the enzyme in a hot water bath at 100°C for 15 minutes, cool to room temperature, filter with a 3kDa ultrafiltration membrane, concentrate and dry the filtrate with less than 3kDa, and obtain a peony root crude extract; suspend the peony root crude extract in an ethanol aqueous solution with a volume concentration of 30%, mix and soak for 20 minutes, filter with a 3kDa ultrafiltration membrane, concentrate and dry the filtrate with less than 3kDa, and obtain a peony root extract;

[0119] S1.5, Preparation of Baobab Fruit Extract:

[0120] In S1 of the above preparation method, the preparation method of the baobab pulp extract is:

[0121] Baobab pulp is taken, crushed, added to water with an amount 8 times the mass, then bifidobacteria with an amount 1% by mass of the baobab pulp is added, fermented at 42° C. for 12 hours, sterilized in a boiling water bath at 100° C. for 15 minutes, and cooled to room temperature to obtain a fermentation liquid; anhydrous ethanol with an amount 3 times the mass of the baobab pulp is added to the fermentation liquid, mixed and soaked for 20 minutes, filtered using a 3 kDa ultrafiltration membrane, and the filtrate with an amount less than 3 kDa is concentrated and dried to obtain a baobab pulp extract;

[0122] S2, preparation of modified sodium DNA:

[0123] S2.1: Dissolve soybean lecithin and cholesterol uniformly in anhydrous ethanol at a molar ratio of soybean lecithin to cholesterol = 5:1, and perform rotary evaporation at 55°C to form a thin film on the container wall to obtain a thin film;

[0124] S2.2: Peach gum powder was dissolved in phosphate buffered saline (PBS) with a mass ratio of peach gum powder to sodium DNA = 1:10, and then sodium DNA was added. After mixing evenly, a 5% sodium DNA film was added, and ultrasound was performed at 60KHz for 15 minutes in a 4°C water bath to obtain an emulsion;

[0125] S2.3: The emulsion is placed in a syringe and repeatedly pushed out through a 0.30 μm polycarbonate filter to obtain a nanoemulsion and obtain modified DNA sodium;

[0126] S3, preparing essence:

[0127] S3.1: Prepare, by mass, 1 part of liquorice extract, 3 parts of Centella asiatica extract, 0.5 parts of feverfew extract, 2 parts of peony root extract, 2 parts of baobab pulp extract, 1 part of palmitoyl hexapeptide-14, 0.5 parts of tetrahydromethylpyrimidine carboxylic acid, 1 part of modified sodium DNA, 0.5 parts of isopentyl glycol and 50 parts of purified water;

[0128] S3.2: Heat purified water to 50°C according to mass proportions, add tetrahydromethylpyrimidine carboxylic acid, modified sodium DNA and isoprene glycol and mix evenly, then cool to 45°C, add licorice extract, Centella asiatica extract, feverfew extract, peony root extract, baobab pulp extract and palmitoyl hexapeptide-14, mix evenly to obtain an essence.

[0129] Example 5

[0130] A method for preparing a skin repair and anti-allergic essence comprises the following steps:

[0131] S1, preparation of plant extracts:

[0132] S1.1, preparation of licorice extract:

[0133] The licorice is crushed, anhydrous ethanol is added in an amount 3 times the weight of the licorice, and soaked for 30 minutes. The sieve liquid is filtered through a 325-mesh screen to obtain the sieve liquid, and the sieve liquid is concentrated and dried to obtain a licorice crude extract; the licorice crude extract is suspended in a pH 7.0 glucose aqueous solution with a glucose content of 2 g / L, 1% of the weight of the licorice crude extract of Bacillus subtilis and 0.2% of the weight of the licorice crude extract of serine protease are added, and the licorice crude extract is cultured at 36°C for 20 hours to obtain a fermentation hydrolyzate, and the fermentation hydrolyzate is sterilized and inactivated in a hot water bath at 90°C for 15 minutes. After the fermentation hydrolyzate is cooled to room temperature, a 3 kDa ultrafiltration membrane is used to filter the fermentation hydrolyzate, and a filtrate with a weight less than 3 kDa is concentrated and dried to obtain a licorice extract.

[0134] S1.2, preparation of Centella asiatica extract:

[0135] The method comprises the following steps: grinding Centella asiatica, adding 6 times the mass of water, adjusting the pH value to 5.0 with hydrochloric acid, adding 1% by mass of hemicellulase of Centella asiatica, performing enzymolysis at 50°C for 1.5 hours, inactivating the enzyme in a hot water bath, cooling to room temperature, filtering with a 5kDa ultrafiltration membrane, concentrating and drying the filtrate with a mass less than 5kDa, and obtaining a crude Centella asiatica extract; suspending the crude Centella asiatica extract in 6 times the mass of water, and then adding 0.5% by mass of aspartic acid protease and 0.5% by mass of cysteine ​​protease of the crude Centella asiatica extract, performing enzymolysis at 37°C for 1.5 hours, inactivating the enzyme in a hot water bath at 90°C for 15 minutes, cooling to room temperature, filtering with a 3kDa ultrafiltration membrane, concentrating and drying the filtrate with a mass less than 3kDa, and obtaining a Centella asiatica extract.

[0136] S1.3, preparation of feverfew extract:

[0137] Crush feverfew, add 5 times the mass of water, soak in 50°C hot water for 60 minutes, cool to room temperature, add 1 times the mass of anhydrous ethanol of feverfew, continue soaking for 30 minutes, filter with a 5kDa ultrafiltration membrane, take the filtrate less than 5kDa, concentrate and dry, and obtain feverfew extract.

[0138] S1.4, preparation of peony root extract:

[0139] Wash and crush the peony root, add 6 times the weight of water, adjust the pH to 6.0, add 2% hemicellulase and 1% papain of the peony root, perform enzymolysis at 40°C for 1.5 hours, inactivate the enzyme in a hot water bath at 90°C for 15 minutes, cool to room temperature, filter with a 3kDa ultrafiltration membrane, concentrate and dry the filtrate with less than 3kDa, and obtain a peony root crude extract; suspend the peony root crude extract in an ethanol aqueous solution with a volume concentration of 20%, mix and soak for 20 minutes, filter with a 3kDa ultrafiltration membrane, concentrate and dry the filtrate with less than 3kDa, and obtain a peony root extract;

[0140] S1.5, Preparation of Baobab Fruit Extract:

[0141] In S1 of the above preparation method, the preparation method of the baobab pulp extract is:

[0142] Baobab pulp is taken, crushed, added to water of 6 times its mass, then bifidobacteria of 1% of the mass of the baobab pulp are added, fermented at 37°C for 12 hours, sterilized in a boiling water bath at 90°C for 15 minutes, and cooled to room temperature to obtain a fermentation liquid; anhydrous ethanol of 2 times the mass of the baobab pulp is added to the fermentation liquid, mixed and soaked for 20 minutes, filtered with a 3kDa ultrafiltration membrane, and the filtrate less than 3kDa is concentrated and dried to obtain a baobab pulp extract;

[0143] S2, preparation of modified sodium DNA:

[0144] S2.1: Dissolve soybean lecithin and cholesterol uniformly in anhydrous ethanol at a molar ratio of soybean lecithin to cholesterol = 2:1, and perform rotary evaporation at 55°C to form a thin film on the container wall to obtain a thin film;

[0145] S2.2: Peach gum powder was dissolved in phosphate buffered saline (PBS) with a mass ratio of 1:5, followed by adding sodium DNA. After mixing well, a 3% sodium DNA film was added, and ultrasonication was performed at 40 kHz for 15 min in a 4°C water bath to obtain an emulsion.

[0146] S2.3: The emulsion is placed in a syringe and repeatedly pushed out through a 0.22 μm polycarbonate filter to obtain a nanoemulsion and obtain modified DNA sodium;

[0147] S3, preparing essence:

[0148] S3.1: Prepare, by mass, 1 part of liquorice extract, 1 part of Centella asiatica extract, 0.5 part of feverfew extract, 0.5 part of peony root extract, 2 parts of baobab pulp extract, 0.5 part of palmitoyl hexapeptide-14, 0.5 part of tetrahydromethylpyrimidine carboxylic acid, 0.8 part of modified DNA sodium, 0.5 part of isopentyl glycol and 30 parts of purified water;

[0149] S3.2: Heat purified water to 50°C according to mass proportions, add tetrahydromethylpyrimidine carboxylic acid, modified sodium DNA and isoprene glycol and mix evenly, then cool to 38°C, add licorice extract, Centella asiatica extract, feverfew extract, peony root extract, baobab pulp extract and palmitoyl hexapeptide-14, mix evenly to obtain an essence.

[0150] Preparation Comparative Example 1

[0151] DNA sodium was not modified, and other parameters were the same as in Example 1.

[0152] Preparation Comparative Example 2

[0153] Licorice extract, Centella asiatica extract, feverfew extract, peony root extract and baobab fruit extract were all soaked in ethanol to extract a filtrate less than 3 kDa, which was concentrated and dried to obtain an extract; other parameters were the same as in Example 1.

[0154] Preparation Comparative Example 3

[0155] No liquorice extract was added, and other parameters were the same as in Example 1.

[0156] Preparation Comparative Example 4

[0157] No Centella asiatica extract was added, and other parameters were the same as in Example 1.

[0158] Preparation Comparative Example 5

[0159] No feverfew extract was added, and other parameters were the same as in Example 1.

[0160] Preparation Comparative Example 6

[0161] No peony root extract was added, and other parameters were the same as in Example 1.

[0162] Preparation Comparative Example 7

[0163] No baobab pulp extract was added, and other parameters were the same as in Example 1.

[0164] 1. The essences of each embodiment and the essences of the comparative examples were subjected to skin occlusive patch tests:

[0165] 60 female subjects (aged 28 to 43) were selected, and various essence test samples were placed in a spot tester. Purified water was used as a blank control, and the spot tester was applied to the curved side of the subject's forearm. After maintaining it for 24 hours, the spot tester was removed and the skin reaction was observed for 48 hours. The results were all negative, with no adverse allergic reactions, indicating that each essence is safe and non-irritating.

[0166] 2. The essences of each embodiment and the comparative example were subjected to stability tests:

[0167] Each essence was sealed and stored at 40°C, 60% humidity, and light environment for 6 months. The results are as follows: the essences of Examples 1 to 5 did not change color, have odor, precipitate, flocculate, etc., and had good fluidity. The essences of Comparative Examples 2 to 7 did not change color, have odor, precipitate, flocculate, etc., and had good fluidity. However, Comparative Example 1 had slight color change, odor, and a small amount of flocculation, showing deterioration characteristics. The inventor speculates that the instability of DNA sodium monomers affected the deterioration of the essence.

[0168] 3. The essences of each embodiment and the comparative example were subjected to anti-allergy tests for inhibiting hyaluronidase:

[0169] Hyaluronidase is involved in allergic reactions and has a strong association with allergies and inflammation. It can inhibit the activity of hyaluronidase, indicating that it has anti-allergic properties. According to the cosmetic anti-allergic test method, (1) prepare 0.26 mol / L calcium chloride solution. (2) Prepare a hyaluronidase solution with a concentration of 1250U / ml, and the solvent of the hyaluronidase solution is an acetic acid buffer solution with a pH of 5.6. (3) Dilute each essence to 3 times the volume with an acetic acid buffer solution with a pH of 5.6, and make three parallel samples for each sample. The prepared solution is the sample solution. (4) Prepare a sodium hyaluronate solution with a concentration of 0.5 mg / ml using an acetic acid buffer solution with a pH of 5.6. (5) Prepare a 0.45 mol / L sodium hydroxide solution. (6) Prepare a 6% acetylacetone solution with a volume concentration of 1.2 mol / L carbonic acid solution. (7) Prepare a 0.04 g / mL p-dimethylaminobenzaldehyde solution with an equal volume ratio of concentrated hydrochloric acid ethanol solution.

[0170] 0.1 ml of calcium chloride solution was mixed with 0.5 ml of hyaluronidase solution, and the mixture was incubated at 37°C for 20 min. Then, 0.3 ml of sample solution was added. After further incubation at 37°C for 20 min, 0.5 ml of sodium hyaluronate solution was added. After further incubation at 37°C for 20 min, 0.1 ml of sodium hydroxide solution and acetylacetone solution were added. The mixture was heated in a boiling water bath for 15 min, cooled to room temperature, p-dimethylaminobenzaldehyde solution was added, and the mixture was allowed to stand for 20 min to develop color. Then, the absorbance at 530 nm was measured, and the hyaluronidase inhibition rate was calculated. The results are shown in Table 1 below.

[0171] Hyaluronidase inhibition rate (%) = [(X1-X2)-(X3-X4)] / (X1-X2)×100%; wherein: X1 is the absorbance value when the acetate buffer solution replaces the sample solution; X2 is the absorbance value when the acetate buffer solution replaces the sample solution and the hyaluronidase solution; X3 is the absorbance value when the sample solution is added; X4 is the absorbance value when the acetate buffer solution replaces the hyaluronidase solution.

[0172] Table 1 Hyaluronidase inhibition rate test results

[0173] Sample Hyaluronidase inhibition rate % Sample Hyaluronidase inhibition rate % Example 1 70.6 Comparative Example 1 70.2 Example 2 69.2 Comparative Example 2 43.3 Example 3 71.3 Comparative Example 3 60.2 Example 4 70.4 Comparative Example 4 65.4 Example 5 68.3 Comparative Example 5 63.1 / / Comparative Example 6 68.3 / / Comparative Example 7 61.7

[0174] From the above results, it can be seen that the plant extracts prepared by fermentation or enzymolysis methods in Examples 1 to 5 have good hyaluronidase inhibition effects, and the hyaluronidase inhibition rate is greatly improved compared to the plant extract extracted by ethanol (Comparative Example 2); according to the analysis of the embodiment and Comparative Example 3 and Comparative Example 7, the licorice extract and the baobab pulp extract prepared by the fermentation or enzymolysis method have a very obvious inhibitory effect on hyaluronidase. The inventor speculates that the plant extracts prepared by the fermentation or enzymolysis method contain rich small molecule polypeptides and other beneficial fermentation or enzymolysis products, have good cell repair, improve the natural barrier of cells, improve immunity, and achieve anti-allergic effects.

[0175] 4. The essences of each embodiment and the comparative example were tested for skin moisture loss (skin barrier repair):

[0176] 120 female subjects (26-42 years old) were selected and divided into 12 groups, with 10 people in each group. The groups used the essence of the embodiment and the essence of the comparative example respectively. The essence was used after cleansing the face in the morning and evening every day for 60 consecutive days. Before and during use, a skin moisture loss tester was used to test the moisture status of the skin, i.e., the transepidermal water loss value TEWL, and the transepidermal water loss value TEWL change rate (%) of the skin was calculated. The test results are shown in Table 2 below.

[0177] Transepidermal water loss value TEWL change rate (%) = (T0-T1) / T0×100%, where T0 is the transepidermal water loss TEWL value of the skin before use, and T1 is the transepidermal water loss TEWL value of the skin after 20 days of use.

[0178] Table 2 Transepidermal water loss TEWL change rate test results

[0179] Sample TEWL change rate % Sample TEWL change rate % Example 1 25.36 Comparative Example 1 18.36 Example 2 24.36 Comparative Example 2 16.23 Example 3 26.58 Comparative Example 3 22.15 Example 4 25.57 Comparative Example 4 23.26 Example 5 27.35 Comparative Example 5 24.68 / / Comparative Example 6 23.25 / / Comparative Example 7 21.03

[0180] It can be seen from the above results that the essences of Examples 1 to 5 have a good moisturizing effect on the skin. According to the examples compared with Comparative Example 1, it can be seen that the modified DNA sodium has a better moisturizing effect. The inventor speculates that this is because the modified DNA sodium is more stable, highly effective, easy to absorb, promotes cells to restore a healthy state and promotes cell division and proliferation, thickens the cortex, forms a natural water-retaining barrier, and repairs the dryness, wrinkles and roughness of the skin very well. According to the examples compared with Comparative Example 2, it can be seen that the plant extracts prepared by fermentation or enzymatic hydrolysis have a good repair and moisturizing effect. The inventor speculates that this is because of the effect of small molecule peptides, which can be used in combination with DNA sodium to accelerate the repair of skin cells and achieve a good moisturizing effect. According to Comparative Examples 3, 4, 6 and 7, various plant extracts have a certain effect of promoting skin moisturizing.

[0181] 5. The essences of the embodiments and the comparative examples were tested for their acne-removing effects:

[0182] 120 volunteers (22-28 years old) with recurrent allergic papules on their faces were selected and divided into 12 groups, with 10 people in each group. Each group used the essence of the embodiment and the essence of the comparative example respectively. After cleansing their faces in the morning and evening every day, the essence was used and the recovery was tracked and observed, as shown in the following Table 3:

[0183] Table 3 Allergy recovery results

[0184]

[0185]

[0186] From the above results, it can be seen that the essences of Examples 1 to 5 have a good effect on allergic acne removal, and can repair the skin more quickly to achieve a healthy state. Among them, the use of various plant extracts in combination with DNA sodium can greatly increase the repair speed. Compared with plant extracts extracted by ethanol, substances extracted by fermentation or enzymatic hydrolysis have a faster repair and acne removal effect, shorten the course of treatment, and have obvious effects; and after 30 days of discontinuation, the skin can restore a good anti-allergic state, form its own barrier, enhance immunity, and prevent allergy recurrence.

Claims

1. A method for preparing a skin repair and anti-allergic essence, characterized in that: The steps include: S1, preparation of plant extracts: including the preparation of liquorice extract, centella asiatica extract, feverfew extract, peony root extract and baobab pulp extract; S2, preparation of modified sodium DNA: S2.1: Dissolve soybean lecithin and cholesterol uniformly in anhydrous ethanol at a molar ratio of soybean lecithin: cholesterol = (2-5): 1, and perform rotary evaporation at 55°C-60°C to form a thin film on the container wall to obtain a thin film; S2.2: Dissolve peach gum powder in phosphate buffered saline (PBS) with a mass ratio of peach gum powder to sodium DNA = 1: (5-10), then add sodium DNA, mix well, add a film with a mass ratio of 3% to 5% of sodium DNA, and ultrasonicate at 40KHz to 60KHz in a water bath at 4℃ to 6℃ for 15min to 20min to obtain an emulsion; S2.3: The emulsion is placed in a syringe and repeatedly pushed out through a 0.22 μm to 0.30 μm polycarbonate filter membrane to obtain a nanoemulsion and obtain modified DNA sodium; S3, prepare essence: S3.1: Prepare, by mass, 1 to 3 parts of liquorice extract, 1 to 3 parts of Centella asiatica extract, 0.5 to 2 parts of feverfew extract, 0.5 to 2 parts of peony root extract, 2 to 5 parts of baobab pulp extract, 0.5 to 1 part of palmitoyl hexapeptide-14, 0.5 to 3 parts of tetrahydromethylpyrimidine carboxylic acid, 0.2 to 3 parts of modified DNA sodium, 0.5 to 3 parts of isopentyl glycol and 30 to 50 parts of purified water; S3.2: Heat purified water to 50°C to 60°C according to mass fractions, add tetrahydromethylpyrimidine carboxylic acid, modified DNA sodium and isoprene glycol and mix evenly, then cool to 38°C to 45°C, add licorice extract, Centella asiatica extract, feverfew extract, peony root extract, baobab pulp extract and palmitoyl hexapeptide-14, mix evenly to obtain an essence; The preparation method of the licorice extract comprises the following steps: crushing licorice, soaking it in anhydrous ethanol, filtering it with a sieve to obtain a sieve liquid, concentrating and drying it to obtain a licorice crude extract; suspending the licorice crude extract in a glucose aqueous solution with a pH of 7.0 to 7.2, adding 1% to 1.5% by weight of Bacillus subtilis of the licorice crude extract and 0.2% to 0.5% by weight of serine protease of the licorice crude extract, culturing it at 36° C. to 38° C. for 20 h to 25 h to obtain a fermentation hydrolyzate, sterilizing and inactivating the enzyme in a hot water bath, cooling it to room temperature, filtering the fermentation hydrolyzate with a 3 kDa ultrafiltration membrane, and concentrating and drying the filtrate with a value less than 3 kDa to obtain a licorice extract; The preparation method of the Centella asiatica extract is as follows: taking Centella asiatica and crushing it, adding 6 to 8 times the mass of water, adjusting the pH to 5.0 to pH 5.5 with hydrochloric acid, adding 1% to 2% of the mass of hemicellulase of Centella asiatica, performing enzymolysis at 50°C to 60°C for 1.5 hours to 2 hours, killing the enzyme in a hot water bath, cooling to room temperature, filtering with a 5kDa ultrafiltration membrane, and concentrating and drying the filtrate less than 5kDa to obtain a crude Centella asiatica extract; suspending the crude Centella asiatica extract in 6 to 8 times the mass of water, and then adding 0.5% to 1% of the mass of aspartic acid protease and 0.5% to 1% of the mass of cysteine ​​protease of the crude Centella asiatica extract, performing enzymolysis at 37°C to 38°C for 1.5 hours to 2 hours, killing the enzyme in a hot water bath, cooling to room temperature, filtering with a 3kDa ultrafiltration membrane, and concentrating and drying the filtrate less than 3kDa to obtain a Centella asiatica extract; The preparation method of the peony root extract comprises the following steps: washing and crushing the peony root, adding 6 to 8 times the weight of water, adjusting the pH to 6.0 to 6.5, adding 2% to 3% by weight of hemicellulase and 1% to 1.5% by weight of papain, performing enzymolysis at 40° C. to 45° C. for 1.5 to 2 hours, inactivating the enzymes by hot water bath, cooling to room temperature, filtering with a 3 kDa ultrafiltration membrane, concentrating and drying the filtrate with a mass less than 3 kDa, and obtaining a crude peony root extract; suspending the crude peony root extract in an ethanol aqueous solution, mixing and soaking, filtering with a 3 kDa ultrafiltration membrane, concentrating and drying the filtrate with a mass less than 3 kDa, and obtaining a peony root extract; The preparation method of the baobab pulp extract comprises the following steps: taking baobab pulp, crushing it, adding it to water of 6 to 8 times its mass, then adding bifidobacteria of 1% to 3% by mass of the baobab pulp, fermenting it at 37 to 42° C. for 12 to 14 hours, sterilizing it in a boiling water bath, and cooling it to room temperature to obtain a fermentation liquid; adding anhydrous ethanol to the fermentation liquid for mixing and soaking, filtering it with a 3 kDa ultrafiltration membrane, and concentrating and drying the filtrate less than 3 kDa to obtain the baobab pulp extract.

2. The method for preparing a skin repairing and anti-allergic essence according to claim 1, characterized in that: In the preparation method of licorice extract, the amount of anhydrous ethanol added is 3 to 6 times the mass of licorice; the soaking time of anhydrous ethanol is 30 minutes to 60 minutes; the mesh size of the sieve is 325 meshes; the amount of pH7.0 to pH7.2 glucose aqueous solution is 5 to 8 times the mass of licorice crude extract, and the glucose content of the glucose aqueous solution is 2 g / L to 3 g / L; the temperature of the hot water bath sterilization and enzyme inactivation is 90°C to 100°C, and the time is 15 minutes to 20 minutes.

3. The method for preparing a skin repairing and anti-allergic essence according to claim 1, characterized in that: In the preparation method of Centella asiatica extract, the temperature of the hot water bath to inactivate the enzyme is 90° C. to 100° C., and the time is 15 min to 20 min.

4. The method for preparing a skin repairing and anti-allergic essence according to claim 1, characterized in that: In S1, the preparation method of the feverfew extract is: Take feverfew and grind it, add 5 to 6 times the mass of water, soak it in hot water at 50 to 60 degrees Celsius for 60 to 90 minutes, cool it to room temperature, add anhydrous ethanol 1 to 3 times the mass of feverfew, continue soaking for 30 to 50 minutes, filter it with a 5kDa ultrafiltration membrane, take the filtrate with a mass less than 5kDa, concentrate and dry it, and obtain the feverfew extract.

5. The method for preparing a skin repairing and anti-allergic essence according to claim 1, characterized in that: In the preparation method of peony root extract, the temperature of the hot water bath to inactivate enzymes is 90°C to 100°C, and the time is 15min to 20min; the ethanol volume concentration of the ethanol aqueous solution is 20% to 30%; and the mixed soaking time is 20min to 30min.

6. The method for preparing a skin repairing and anti-allergic essence according to claim 1, characterized in that: In the preparation method of the baobab pulp extract, the boiling water bath sterilization temperature is 90°C to 100°C, and the time is 15min to 20min; the amount of anhydrous ethanol added is 2 to 3 times the mass of the baobab pulp; and the anhydrous ethanol mixed soaking time is 20min to 30min.

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