A MXene@TiO2 multifunctional hydrogel dressing for treating skin wound repair and its preparation method

Abstract

The present invention discloses a MXene@TiO2 multifunctional hydrogel dressing for treating skin wound repair and a preparation method thereof. The hydrogel dressing is a main hydrogel network formed by the Schiff base reaction of aldehyde-modified konjac gum and hydrazide-modified gelatin. A MXene@TiO2 solution is added to a prepolymer solution of the hydrogel, and a MXene@TiO2 multifunctional hydrogel dressing for treating skin wound repair is obtained by a simple 1:1 mixing in a mold. The MXene@TiO2 multifunctional hydrogel dressing for treating skin wound repair of the present invention has the characteristics of injectability, self-healing properties, adhesion, antibacterial properties, photothermal effect, conductivity and good biocompatibility, etc., which helps to more accurately regulate complex wounds to promote wound repair.

Description

Technical Field

[0001] The invention relates to a preparation method of a MXene@TiO2 multifunctional hydrogel dressing for treating skin wound repair, belonging to a medical antibacterial dressing for promoting skin wound repair. Background Art

[0002] Under normal physiological conditions, human skin has a certain ability to repair and regenerate itself. However, when the skin's structure is damaged, the skin's function is impaired, resulting in wounds. Newly formed wounds are prone to bacterial infection due to exposure to air. Since the inflammatory environment has an inhibitory effect on bacterial growth, without external intervention, the human immune system often spontaneously produces an excessive inflammatory response. Excessive inflammatory response will affect the wound healing process, causing scars to form on the wound or fibrosis to form around the organs. The former causes people cosmetic problems, while the latter may lead to serious functional damage or chronic wounds that cannot heal, resulting in pathological consequences (fibrosis or chronic wounds).

[0003] Hydrogels, natural polymer materials with similar structures to organisms, are the most ideal wound dressings of the 21st century. Hydrogels are similar to native extracellular matrix (ECM) and can better maintain tissue function and promote cell migration; hydrogels are excellent carriers of cells, drugs and biological activities; hydrogels have a high water content, maintain a moist environment for wounds, and can be widely used to close and promote healing of different types of wounds. However, many hydrogels usually focus on one or several functions. For example, skin wound healing requires the coordination of various aspects such as hemostasis, anti-inflammation, and antibacterial. The rapid degradation rate of hydrogels requires repeated administration, and the lack of some characteristics cannot effectively shorten the infection time. Therefore, there is an urgent need to develop multifunctional hydrogels. Summary of the Invention

[0004] The purpose of the present invention is to provide a MXene@TiO2 multifunctional hydrogel dressing for treating skin wound repair and a preparation method thereof. The hydrogel skin dressing has multiple functions such as injectability, self-healing, tissue adhesion, conductivity, antibacterial properties, photothermal effect and hemostasis, which is beneficial to accelerate the patient's wound repair and alleviate the patient's pain.

[0005] The objective of the present invention is achieved by providing a MXene@TiO2 multifunctional hydrogel dressing for treating skin wound repair, characterized in that it is prepared by the following preparation method, specifically comprising the following steps: forming a main hydrogel network by reacting aldehyde-modified konjac gum and hydrazide-modified gelatin through a Schiff base; then adding a MXene@TiO2 solution to a prepolymer solution of the hydrogel, and obtaining the MXene@TiO2 multifunctional hydrogel dressing for treating skin wound repair by simply mixing in a mold at a mass ratio of 1:1.

[0006] The method for preparing a MXene@TiO2 multifunctional hydrogel dressing for treating skin wound repair according to the present invention is characterized in that it comprises the following steps:

[0007] Konjac gum (KGM) was added to deionized water and stirred. After dissolution, sodium periodate (NaIO4) was added and stirred in the dark at 30°C for several hours. Ethylene glycol was then added to terminate the reaction for 2 hours. The mixture was dialyzed against deionized water for 3 days. The supernatant was centrifuged and freeze-dried to obtain the aldehyde-modified KGM product. PBS was then used as the solvent to prepare an 8 wt% aldehyde-modified KGM solution.

[0008] 2) Gelatin was dissolved in morpholineethanesulfonic acid buffer solution. Adipic acid dihydrazide (ADH) was added to the solution. The mixture was reacted in a 50°C water bath for 2 hours, then cooled to room temperature. 1-Ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) and 1-hydroxybenzotriazole (HOBT) were added. The crude product was stirred at room temperature for several hours. The purified sample was then dialyzed against deionized water for 5 days and freeze-dried to obtain hydrazide gelatin. A 16 wt% hydrazide gelatin solution was then prepared using PBS as the solvent.

[0009] 3) First, take 20 mL of 9 M hydrochloric acid into the reactor, then add 1.6 g of lithium fluoride to the hydrochloric acid, and under continuous stirring, slowly add 1 g of Ti3AlC2 powder to the above solution in batches, and stir at 50 ° C for 24 hours to obtain a mixture. Centrifuge the mixture for 5 minutes, pour off the initial etching solution to obtain a precipitate, and then wash the precipitate several times with dilute hydrochloric acid and deionized water until the pH of the supernatant in the precipitate reaches 6; this precipitate (Ti3C2T x ) were dispersed in deionized water, placed in an ultrasonic cleaner, protected by inert gas and ultrasonicated on ice for 2 h. After ultrasonication, the supernatant was collected by centrifugation, and the obtained supernatant was hydrothermally treated and finally dried in a vacuum drying oven at 50 ° C for 48 h to obtain MXene@TiO2 powder;

[0010] 4) The MXene@TiO2 powder from step 3) was dissolved in PBS (pH = 7.4), and then ultrasonicated in an ultrasonic cleaner to obtain a MXene@TiO2 solution with a concentration of 0.5 mg / mL;

[0011] 5) dissolving the aldehyde-modified konjac gum solution obtained in step 1) and the hydrazide-modified gelatin obtained in step 2) in the MXene@TiO2 solution obtained in step 4) to obtain a hydrogel prepolymer solution;

[0012] 6) injecting the hydrogel prepolymer obtained in step 5) into the mold and waiting for a few minutes at room temperature to obtain the hydrogel, or directly forming the hydrogel on the wound surface using a double-barreled syringe;

[0013] 7) placing the hydrogel obtained by injecting into the mold in step 6) into a centrifuge tube and freeze-drying the resulting dry hydrogel;

[0014] 8) The dried hydrogel obtained in step 7) is placed in PBS to achieve swelling equilibrium, thereby obtaining a MXene@TiO2 multifunctional hydrogel dressing for treating skin wound repair.

[0015] Furthermore, in the above step 1), the ratio of konjac gum to deionized water is 1-1.5 g:100 mL, and the ratio of sodium periodate to deionized water is 0.2-0.3 g:100 mL.

[0016] Furthermore, in step 2) above, the ratio of gelatin to morpholineethanesulfonic acid buffer solution is 1-1.5 g:100 mL, the ratio of adipic acid dihydrazide to morpholineethanesulfonic acid buffer solution is 1.1 g-1.3 g:100 mL, the ratio of 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) to morpholineethanesulfonic acid buffer solution is 0.25-0.3 g:100 mL, and the ratio of 1-hydroxybenzotriazole (HOBT) to morpholineethanesulfonic acid buffer solution is 0.15-0.25 g:100 mL.

[0017] Furthermore, the dilute hydrochloric acid in step 3) is 1 M, and the mixture is centrifuged for 5 minutes at a centrifugal speed of 3500 rpm. After the sonication, the supernatant is collected by centrifugation at 5000 rpm for 30 minutes in a low-temperature high-speed centrifuge to collect the black supernatant.

[0018] The MXene@TiO2 multifunctional hydrogel dressing for treating skin wound repair is prepared by the above-mentioned preparation method of the present invention.

[0019] Specifically, the present invention provides a MXene@TiO2 multifunctional hydrogel dressing for treating skin wounds. The dressing comprises a main hydrogel network formed by a Schiff base reaction between aldehyde-modified konjac gum and hydrazide-modified gelatin. A MXene@TiO2 solution is added to a prepolymer of the hydrogel. The mixture is then freeze-dried in a mold at a simple 1:1 mass ratio and stored. The freeze-dried hydrogel rapidly absorbs water and swells in PBS, resulting in a MXene@TiO2 multifunctional hydrogel dressing for treating skin wounds.

[0020] The present invention provides a method for preparing a MXene@TiO2 multifunctional hydrogel dressing for treating skin wound repair, comprising the following steps:

[0021] Step 1: Add konjac gum (KGM) to deionized water and stir. After dissolving, add sodium periodate (NaIO4) and stir in the dark at 30°C for several hours. Then add ethylene glycol to terminate the reaction for 2 hours. Then dialyze with deionized water for 3 days. After centrifugation, obtain the supernatant and freeze-dry to obtain the aldehyde-modified konjac gum product.

[0022] Step 2: Dissolve gelatin in morpholineethanesulfonic acid buffer. Add adipic acid dihydrazide (ADH) to the solution, react in a 50°C water bath for 2 hours, and then cool to room temperature. Add 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) and 1-hydroxybenzotriazole (HOBT), and stir at room temperature for several hours to obtain a crude product. The purified sample is then dialyzed against deionized water for 5 days and freeze-dried to obtain hydrazide-treated gelatin.

[0023] Step 3: Take 20 mL of 9 M hydrochloric acid into the reactor, then add 1.6 g of lithium fluoride to the hydrochloric acid and continue stirring. Slowly add 1 g of Ti3AlC2 powder to the above solution in batches and stir at 50°C for 24 hours. Centrifuge the mixture for 5 minutes and discard the initial etching solution to obtain Ti3C2T x The Ti3C2T was precipitated and then washed with dilute hydrochloric acid and deionized water. x Precipitation was performed several times until the pH of the supernatant reached 6. x The precipitate was dispersed in deionized water and placed in an ultrasonic bath under inert gas for 2 hours. The supernatant was collected by centrifugation. The supernatant was hydrothermally treated and finally dried in a vacuum oven at 50°C for 48 hours to obtain MXene@TiO2 powder.

[0024] Step 4: Dissolve the MXene@TiO2 powder in PBS (pH = 7.4) and ultrasonicate in an ultrasonic cleaner to obtain a MXene@TiO2 solution with a concentration of 0.5 mg / mL.

[0025] Step 5: dissolve the aldehyde-modified konjac gum product obtained in the first step and the hydrazide-modified gelatin obtained in the second step in the MXene@TiO2 solution (e.g., 1 mg / mL) obtained in the fourth step to obtain a hydrogel prepolymer solution;

[0026] Step 6: Inject the hydrogel prepolymer obtained in step 5 into the mold and wait for a few minutes at room temperature to obtain the hydrogel, or use a double-barreled syringe to directly form the gel on the wound surface;

[0027] Step 7: Place the hydrogel obtained by injecting into the mold in step 6 into a centrifuge tube and freeze-dry to obtain a dry hydrogel;

[0028] Step 8: Place the dried hydrogel obtained in step 7 in PBS for approximately 10 hours to reach swelling equilibrium, thereby obtaining a MXene@TiO2 multifunctional hydrogel dressing for treating skin wound repair.

[0029] Furthermore, the concentration of the hydrazide gelatin in the second step is 10wt%-14wt%.

[0030] Furthermore, the concentration of the MXene@TiO2 solution in the third step is 0.125 mg / mL-1 mg / mL.

[0031] The inventive principle of this invention is that after skin damage, its wound defects vary. The injectable nature of this hydrogel can perfectly fill these defects. Based on the dynamic hydrogel network generated by the Schiff base reaction, the hydrogel can be restored to a complete, gel-like state when subjected to pressure, exhibiting excellent self-healing properties. The aldehyde groups on the oxidized konjac gum on the hydrogel backbone can form chemical bonds with amino groups on the skin surface, exhibiting excellent tissue adhesion and coating the wound, maintaining a favorable environment for wound repair. During wound repair, wounds are susceptible to external bacterial infection. Therefore, the anionic properties of the MXene@TiO2 surface and its high hydrophilicity, based on a direct contact killing mechanism, enhance contact with the bacterial membrane surface, inactivating adherent microorganisms. Furthermore, hydrogen bonds between the oxygen-containing groups on the surface and the lipopolysaccharide chains on the cell membrane can inhibit bacteria by preventing nutrient intake, thus exhibiting antibacterial properties. Since skin is sensitive to electrical stimulation, the excellent electrical conductivity of MXene@TiO2 can enhance electrical transmission and establish a cellular communication network, thereby improving the quality of wound healing and skin regeneration. In addition, TiO2 on MXene@TiO2 helps stop bleeding, thereby accelerating wound healing.

[0032] The beneficial effects of this invention include: the MXene@TiO2 multifunctional hydrogel dressing for skin wound repair exhibits excellent biocompatibility. After covering a skin wound, the hydrogel releases MXene@TiO2 to rapidly kill bacteria and sterilize it, while also absorbing tissue permeate. OKGM stimulates fibroblast proliferation and immune regulation, accelerating the inflammatory and remodeling phases of wound repair. The hydrogel can be injected directly or freeze-dried in a mold for storage, soaking in PBS before use. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 This is a synthetic route for connecting oxidized konjac gum and hydrazide gelatin via a Schiff base reaction.

[0034] Figure 2 The synthesis route of single-layer MXene@TiO2 nanomaterials and the corresponding SEM image.

[0035] Figure 3 This is the XRD pattern of MXene@TiO2. DETAILED DESCRIPTION

[0036] The technical solutions of the present invention are further described below with reference to the following examples, but these examples are not intended to limit the present invention. Example 1

[0037] Step 1: 5 g of konjac gum (KGM) was added to 500 mL of deionized water and stirred. After dissolution, 1.32 g of sodium periodate (NaIO4) was added and stirred in the dark at 30°C for several hours. Ethylene glycol was then added to terminate the reaction for 2 hours. The mixture was then dialyzed with deionized water for 3 days. The supernatant was obtained after centrifugation and freeze-dried to obtain the aldehyde-modified konjac gum product.

[0038] Step 2: Dissolve 2g of gelatin in 200mL of morpholineethanesulfonic acid buffer (pH 6.5). Add 2.3g of adipic acid dihydrazide (ADH) to the solution, react in a 50°C waterbath for 2 hours, and then cool to room temperature. Add 0.5066g of 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) and 0.357g of 1-hydroxybenzotriazole (HOBT), and stir at room temperature for several hours to obtain a crude product. The purified sample is then dialyzed against deionized water for 5 days and freeze-dried to obtain hydrazide-treated gelatin.

[0039] Step 3: Take 20 mL of 9 M hydrochloric acid into the reactor, then add 1.6 g of lithium fluoride to the hydrochloric acid and continue stirring. Slowly add 1 g of Ti3AlC2 powder to the above solution in batches and stir at 50°C for 24 hours. Centrifuge the mixture for 5 minutes and discard the initial etching solution to obtain Ti3C2T x The Ti3C2T was precipitated and then washed with dilute hydrochloric acid and deionized water. x Precipitation was performed several times until the pH of the supernatant reached 6. x The precipitate was dispersed in deionized water and placed in an ultrasonic bath under inert gas for 2 hours. The supernatant was collected by centrifugation. The supernatant was hydrothermally treated and finally dried in a vacuum oven at 50°C for 48 hours to obtain MXene@TiO2 powder.

[0040] Step 4: Weigh 10 mg of the MXene@TiO2 powder from step 3 and dissolve it in 10 mL of PBS (pH 7.4). Ultrasonicate in an ultrasonic cleaner to obtain a 1 mg / mL MXene@TiO2 solution.

[0041] Step 5: Dissolve the aldehyde-modified konjac gum product obtained in the first step and the hydrazide-modified gelatin obtained in the second step in the MXene@TiO2 solution obtained in the fourth step, and heat with a hair dryer for a few minutes to obtain a hydrogel prepolymer solution;

[0042] Step 6: Inject the hydrogel prepolymer obtained in step 5 into a mold with a diameter of 1.1 cm and wait for a few minutes at room temperature to obtain a hydrogel;

[0043] Step 7: Place the hydrogel obtained by injecting into the mold in step 6 into a centrifuge tube, freeze it in a -20°C refrigerator for 24 hours, and dry it in a freeze dryer for 48 hours to obtain a dry hydrogel;

[0044] Step 8: Place the dried hydrogel obtained in step 7 in 1 mL of PBS for approximately 10 hours to reach swelling equilibrium, thereby obtaining a MXene@TiO2 multifunctional hydrogel dressing for treating skin wound repair. Example 2

[0045] Step 1: 5 g of konjac gum (KGM) was added to 500 mL of deionized water and stirred. After dissolution, 1.4 g of sodium periodate (NaIO4) was added and stirred in the dark at 30°C for several hours. Ethylene glycol was then added to terminate the reaction for 2 hours. The mixture was then dialyzed with deionized water for 3 days. The supernatant was obtained after centrifugation and freeze-dried to obtain the aldehyde-modified konjac gum product.

[0046] Step 2: Dissolve 2g of gelatin in 200mL of morpholineethanesulfonic acid buffer (pH 6.5). Add 2.5g of adipic acid dihydrazide (ADH) to the solution, react in a 50°C waterbath for 2 hours, and then cool to room temperature. Add 0.52g of 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) and 0.38g of 1-hydroxybenzotriazole (HOBT), and stir at room temperature for several hours to obtain a crude product. The purified sample is then dialyzed against deionized water for 5 days and freeze-dried to obtain hydrazide-treated gelatin.

[0047] Step 3: Take 20 mL of 9 M hydrochloric acid into the reactor, then add 1.6 g of lithium fluoride to the hydrochloric acid and continue stirring. Slowly add 1 g of Ti3AlC2 powder to the above solution in batches and stir at 50°C for 24 hours. Centrifuge the mixture for 5 minutes and discard the initial etching solution to obtain Ti3C2T x The Ti3C2T was precipitated and then washed with dilute hydrochloric acid and deionized water. x Precipitation was performed several times until the pH of the supernatant reached 6. xThe precipitate was dispersed in deionized water and placed in an ultrasonic bath under inert gas for 2 hours. The supernatant was collected by centrifugation. The supernatant was hydrothermally treated and finally dried in a vacuum oven at 50°C for 48 hours to obtain MXene@TiO2 powder.

[0048] Step 4: Weigh 5 mg of the MXene@TiO2 powder from step 3 and dissolve it in 10 mL of PBS (pH = 7.4). Ultrasonicate in an ultrasonic cleaner to obtain a 0.5 mg / mL MXene@TiO2 solution.

[0049] Step 5: Dissolve the aldehyde-modified konjac gum product obtained in the first step and the hydrazide-modified gelatin obtained in the second step in the MXene@TiO2 solution obtained in the fourth step, and heat with a hair dryer for a few minutes to obtain a hydrogel prepolymer solution;

[0050] Step 6: Inject the hydrogel prepolymer obtained in step 5 into The hydrogel was obtained after waiting for 20 minutes at room temperature.

[0051] Step 7: Place the hydrogel obtained by injecting into the mold in step 6 into a culture dish, freeze it in a -20°C refrigerator for 24 hours, and dry it in a freeze dryer for 48 hours to obtain a dry hydrogel;

[0052] Step 8: Place the dried hydrogel obtained in step 7 in 20 mL of PBS for approximately 10 hours to reach swelling equilibrium, thereby obtaining a MXene@TiO2 multifunctional hydrogel dressing for treating skin wound repair.

[0053] The following are the experimental results of using this hydrogel dressing in a mouse back skin defect infection model. The skin defect infection model was established using C57BL / 6 mice. A full-thickness skin defect with a diameter of approximately 0.8 cm was made on the mouse back, and 0.05 mL of 2×10 8CFU / mL methicillin-resistant Staphylococcus aureus liquid was used. After the liquid was completely absorbed and dried, the hydrogel dressing of Example 2 of the present application was covered on the wound surface, and the hydrogel was fixed with a 3M Tegaderm dressing. A negative control group was set up, and the defect infection model was not treated, and the wound was covered with a 3M Tegaderm dressing. A positive control group was also set up, and Bactroban mupirocin ointment was applied to the defect infection site, and the wound was covered with a 3M Tegaderm dressing. The results showed that after 3 days, with the negative control as the reference, the bactericidal rates of the hydrogel dressing of the present application and the mupirocin positive control were 71.4% and 73.1%, respectively; after 8 days, the bactericidal rates were 99.1% and 98.7%. The wound healing rate results showed that after 9 days, the wound area healing rates of the negative control, positive control and the hydrogel of the present invention were 1.9%, 49.4% and 65.9%, respectively. The average time required for complete wound healing of the negative control, positive control and the hydrogel of the present application was 19.3 days, 14 days and 9.6 days, respectively.

Claims

1. A method for preparing a MXene@TiO2 multifunctional hydrogel dressing for treating skin wound repair, characterized in that: The steps include: 1) Konjac glucomannan was added to deionized water and stirred. After dissolution, sodium periodate (NaIO4) was added and stirred in the dark at 30°C for several hours. Ethylene glycol was then added and reacted for 2 hours to terminate the reaction. The mixture was then dialyzed with deionized water for 3 days. The supernatant was obtained after centrifugation and freeze-dried to obtain the aldehyde-modified konjac glucomannan product. PBS was then used as a solvent to prepare an aldehyde-modified konjac glucomannan solution with a concentration of 8 wt%; 2) Gelatin was dissolved in morpholineethanesulfonic acid buffer solution. Adipic acid dihydrazide was added to the solution. The mixture was reacted in a water bath at 50°C for 2 hours, then cooled to room temperature. 1-Ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) and 1-hydroxybenzotriazole (HOBT) were added. The mixture was stirred at room temperature for several hours to obtain a crude product. The purified sample was then dialyzed in deionized water for 5 days, and freeze-dried to obtain hydrazide gelatin. A 16 wt% hydrazide gelatin solution was then prepared using PBS as a solvent. 3) First, take 20mL of 9mol / L hydrochloric acid into the reactor, then add 1.6g of lithium fluoride to the hydrochloric acid, and slowly add 1g of Ti3AlC2 powder to the above solution in batches while stirring continuously. Stir at 50℃ for 24 hours to obtain a mixture. Centrifuge the mixture for 5 minutes, pour out the initial etching solution to obtain a precipitate, and then wash the precipitate several times with dilute hydrochloric acid and deionized water until the pH of the supernatant in the precipitate reaches 6; x The product was dispersed in deionized water, placed in an ultrasonic cleaner, protected by inert gas, and ultrasonicated in an ice bath for 2 hours. After ultrasonication, the supernatant was collected by centrifugation, and the obtained supernatant was hydrothermally treated and finally dried in a vacuum drying oven at 50°C for 48 hours to obtain MXene@TiO2 powder. 4) The MXene@TiO2 powder from step 3) was dissolved in PBS (pH = 7.4), and then ultrasonicated in an ultrasonic cleaner to obtain a MXene@TiO2 solution with a concentration of 0.5 mg / mL; 5) dissolving the aldehyde-modified konjac gum solution obtained in step 1) and the hydrazide-modified gelatin obtained in step 2) in the MXene@TiO2 solution obtained in step 4) to obtain a hydrogel prepolymer solution; 6) injecting the hydrogel prepolymer obtained in step 5) into the mold and waiting for a few minutes at room temperature to obtain the hydrogel, or directly forming the hydrogel on the wound surface using a double-barreled syringe; 7) placing the hydrogel obtained by injecting into the mold in step 6) into a centrifuge tube and freeze-drying the resulting dry hydrogel; 8) The dried hydrogel obtained in step 7) is placed in PBS to achieve swelling equilibrium, thereby obtaining a MXene@TiO2 multifunctional hydrogel dressing for treating skin wound repair.

2. The method for preparing a MXene@TiO2 multifunctional hydrogel dressing for treating skin wound repair according to claim 1, characterized in that: In step 1), the ratio of konjac gum to deionized water is 1-1.5 g:100 mL, and the ratio of sodium periodate to deionized water is 0.2-0.3 g:100 mL.

3. The method for preparing a MXene@TiO2 multifunctional hydrogel dressing for treating skin wound repair according to claim 1, characterized in that: In step 2), the ratio of gelatin to morpholineethanesulfonic acid buffer solution is 1-1.5 g:100 mL, the ratio of adipic acid dihydrazide to morpholineethanesulfonic acid buffer solution is 1.1 g-1.3 g:100 mL, the ratio of 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) to morpholineethanesulfonic acid buffer solution is 0.25-0.3 g:100 mL, and the ratio of 1-hydroxybenzotriazole (HOBT) to morpholineethanesulfonic acid buffer solution is 0.15-0.25 g:100 mL.

4. The method for preparing a MXene@TiO2 multifunctional hydrogel dressing for treating skin wound repair according to claim 1, characterized in that: The dilute hydrochloric acid in step 3) is 1 mol / L, and the mixture is centrifuged for 5 minutes at a centrifugal speed of 3500 rpm. After the sonication, the supernatant is collected by centrifugation at 5000 rpm for 30 minutes in a low-temperature high-speed centrifuge to collect the black supernatant.

5. A MXene@TiO2 multifunctional hydrogel dressing for treating skin wound repair prepared by the preparation method according to any one of claims 1 to 4.

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