Preparation technology and application of liquid organic fertilizer

Abstract

The present invention relates to the field of organic fertilizer, and in particular to a preparation process and application of liquid organic fertilizer, wherein the specific preparation process is as follows: solid-liquid separation is performed after collecting animal excrement, and urine and feces are separated; the separated urine is fermented in anaerobic, aerobic and facultative anaerobic environments for 10 days respectively, and then the urine is heated and degraded, and then the temperature is kept constant at 45-55°C, fermentation bacteria, functional bacteria, and carbon element microbial raw materials are added to fully explain, biofermentate and blend the elements, and finally composite deodorizing bacteria are added, and liquid organic fertilizer is obtained after sufficient stirring. The liquid organic fertilizer prepared by the present invention improves the utilization rate of animal excrement, has the functions of improving soil structure and providing plant nutrition, and has the functions of biological control of insect and weed pests, stimulating plant growth, and increasing crop yield.

Description

Technical Field

[0001] The invention belongs to the field of organic fertilizer, and in particular relates to a preparation process and application of liquid organic fertilizer. Background Art

[0002] In modern agricultural production, fertilizer is one of the important factors to ensure high yield and quality of crops. Traditionally, chemical fertilizers are widely used because of their high nutrient concentration and rapid release of fertilizer effect. However, the long-term and large-scale application of chemical fertilizers has caused serious environmental problems and soil degradation. The nutrients in chemical fertilizers are often lost because plants cannot fully absorb them in time, leading to surface and groundwater pollution. At the same time, excessive salt accumulation can also lead to soil structure damage, soil compaction and reduced biodiversity.

[0003] In contrast, organic fertilizers, especially liquid organic fertilizers, are gradually favored by agricultural producers for their unique advantages. Not only do they come from nature and recycle organic waste, but they are also rich in nutrients and beneficial microorganisms, which can gradually improve soil structure and enhance soil fertility. Specifically, organic fertilizers can promote the formation of soil aggregate structure, improve soil water and fertilizer retention capacity, and enhance crop resistance to diseases and pests through the activities of beneficial microorganisms. In addition, because of its more gentle nutrient release, it can effectively reduce nutrient loss and improve fertilizer utilization.

[0004] However, there are still some limiting factors in the actual application of liquid organic fertilizers, such as the slow start of fertilizer effect, which is difficult to match the quick effect of chemical fertilizers in the short term; the relatively low nutrient content, which may require more sophisticated dosage control and fertilization strategies; and the stability of product quality needs to be improved, making farmers less predictable about its effects than chemical fertilizers. These factors work together to result in the use rate of liquid organic fertilizers on crops being lower than that of chemical fertilizers. Summary of the invention

[0005] In order to overcome the shortcomings of the prior art, the present invention aims to solve the problem of how to efficiently process organic waste such as animal urine and convert it into efficient liquid organic fertilizer. At the same time, through technological innovation, it can achieve rapid release of nutrients, improve stability and predictability, so as to achieve the goals of weight loss and efficiency improvement, green and efficient, and quality improvement and income increase.

[0006] A first object of the present invention is to provide a preparation technology of a liquid organic fertilizer, comprising the following steps:

[0007] Step S1: collecting animal excrement and performing solid-liquid separation to separate urine and feces;

[0008] Step S2: The separated urine is placed in an anaerobic environment for 10 days for static fermentation, and then placed in an aerobic and facultative anaerobic environment for 10 days for static aeration fermentation to obtain pretreated urine; this step allows the macromolecular organic matter in the urine to be fully degraded.

[0009] Step S3: heating the pre-treated urine while stirring intermittently, and stopping heating when the urine temperature rises to 80° C., and obtaining thermally degraded urine when the urine temperature drops to 45° C.;

[0010] The specific indirect stirring procedure is: work for 30 minutes, stop for 10 minutes intermittently, and repeat this process. After the full degradation of this step, the E. coli, roundworm eggs and other harmful bacteria and heavy metals in the urine are eliminated, and the macromolecular organic matter is fully degraded again and converted into small molecule soluble organic matter.

[0011] Step S4: the thermally degradable urine is kept at a constant temperature of 45-55° C., fermentation bacteria are added, the fermentation bacteria and the thermally degradable urine are fully mixed, and high-speed stirring is continued for 3 days to obtain fermented and degraded urine; through high-speed stirring and sufficient aerobic decomposition process, the macromolecular organic matter in the urine is further decomposed, and it is also beneficial to remove the unpleasant taste such as hydrogen sulfide contained in the urine.

[0012] Step S5: Under an anaerobic environment, the fermented and degraded urine is kept at a constant temperature of 45-55° C., functional bacteria are added, and the mixture is fully stirred for 24 hours. At this time, the urine has been efficiently converted into biological organic fertilizer within 24 hours. Then, carbon element microbial raw materials are added under an anaerobic state to mix the elements, and the mixture is stirred evenly to promote microbial reproduction, increase the fertilizer efficiency and biological activity of the biological organic fertilizer, achieve the effect of dissolving potassium and phosphorus, and obtain anaerobic fermented urine;

[0013] Step S6: To ensure that the microorganisms in the organic fertilizer are in a biologically active state, the anaerobic fermented urine is placed in a semi-anaerobic and facultative anaerobic environment, and composite deodorizing bacteria are added to remove the mixed odor of ammonia and nitrogen produced in the process. After sufficient stirring, liquid organic fertilizer is obtained.

[0014] Preferably, the fermentation bacteria is a mixture of microorganisms such as Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, Streptomyces rocheri, Aspergillus oryzae, Bacillus amyloliquefaciens, lactic acid bacteria, phosphate-soluble bacteria, Trichoderma viride, Bacillus stearothermophilus licheniformis, Trichoderma viride, Bacillus stearothermophilus licheniformis and the like.

[0015] Preferably, among the fermentation bacteria, the mass ratio of Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, Streptomyces rocheri, Aspergillus oryzae, Bacillus amyloliquefaciens, lactic acid bacteria, phosphate-soluble bacteria, Trichoderma viride, and Bacillus stearothermophilus licheniformis is 20-30:5-10:1-5:10-15:5-10:10-15:1-5:1-5:1-5:1-5:1-5.

[0016] Preferably, the functional bacteria is a mixture of microorganisms such as Bacillus subtilis, Brevibacillus laterosporus, Bacillus amyloliquefaciens, Bacillus licheniformis and Bacillus gelatinus.

[0017] Preferably, in the functional bacteria, the mass ratio of Bacillus subtilis, Brevibacillus laterosporus, Bacillus amyloliquefaciens, Bacillus licheniformis and Bacillus gelatinosa is 30-40:10-20:10-20:10-20:10-20.

[0018] Preferably, the carbon element microbial raw material includes mineral source potassium fulvic acid, humic acid and sugar rice flour, and the mass ratio of the mineral source potassium fulvic acid, humic acid and sugar rice flour is 1-10:10-20:10-15.

[0019] Preferably, the composite deodorizing bacteria includes Bacillus subtilis, Lactobacillus plantarum, photosynthetic bacteria, Bacillus licheniformis, yeast and Enterococcus faecalis, and the mass ratio of Bacillus subtilis, Lactobacillus plantarum, photosynthetic bacteria, Bacillus licheniformis, yeast and Enterococcus faecalis is 1-5:30-40:1-5:1-5:1-5:1-5:1-5.

[0020] Preferably, the added amount of the fermentation bacteria is 0.03-0.04% of the total amount of urine after separation in step S1, the added amount of the functional bacteria is 0.10-0.15% of the total amount of urine after separation in step S1, the added amount of the carbon element microbial raw material is 0.10-0.12% of the total amount of urine after separation in step S1, and the added amount of the composite deodorizing bacteria is 0.18-0.22% of the total amount of urine after separation in step S1.

[0021] Preferably, in step S1, the feces residue can be treated as follows: the feces residue is added with auxiliary materials and a specific composite microbial enzyme preparation for anaerobic fermentation biological treatment; wherein, during the anaerobic fermentation biological treatment process, the temperature is controlled at 50-75°C, the pile is turned once every 2 days, and the fermentation time is 15 days, so as to obtain solid organic fertilizer.

[0022] Another object of the present invention is to provide an application of liquid organic fertilizer in improving farmland and soil, wherein the liquid organic fertilizer can be diluted 2-15 times for use according to different crops to be applied.

[0023] The liquid organic fertilizer of the present invention is suitable for crops such as rice, wheat, corn, cereals, potato crops, bean crops, rapeseed, peanuts, sunflowers and other oil crops, cotton, sugar crops, hemp crops, eggplant vegetables, melon vegetables, root vegetables, potato vegetables, cabbage vegetables, kale vegetables, leafy vegetables, bean vegetables, onion and garlic vegetables, perennial vegetables, aquatic vegetables, citrus fruits, melons and fruits, berries, drupes, pome fruits, nuts, tropical fruits, ornamental flowers, ornamental foliage flowers, ornamental fruit flowers, ornamental stem flowers, ornamental bud flowers, woody Chinese medicinal materials, herbal Chinese medicinal materials, shade-loving Chinese medicinal materials, tea and tobacco, other hobby crops, gardening crops and the like, and has a wide range of applications.

[0024] Beneficial effects:

[0025] The present invention adopts bioengineering means to treat animal urine harmlessly, reduce environmental pollution, and achieve the purpose of recycling waste excrement resources. On the one hand, it quickly eliminates a large number of harmful bacteria contained in urine, and on the other hand, it fully degrades the macromolecular organic matter in urine, targets and adjusts the balance, promotes the growth and reproduction of microorganisms, increases the fertilizer efficiency and biological activity of liquid organic fertilizer, and ensures the safety and stability of the final product, achieving the effect of "reducing weight and increasing efficiency, being green and efficient, and improving quality and increasing income".

[0026] The liquid organic fertilizer prepared by the invention improves the utilization rate of animal excrement, has the functions of improving soil structure and providing plant nutrition, and also has the functions of biologically controlling insect and weed pests, stimulating plant growth and increasing crop yield.

[0027] Instruction Manual

[0028] Figure 1 This is a comparison of Nicotiana tabacum leaves after applying the liquid organic fertilizer of the present application (left) and the fertilizer of Comparative Example 1 (right);

[0029] Figure 2 The picture is a comparison of the actual rice planting conditions of applying the liquid organic fertilizer of the present application (left) and applying the fertilizer of Comparative Example 2 (right);

[0030] Figure 3 The growth of rice after applying the fertilizer of Comparative Example 2;

[0031] Figure 4 The growth of rice after applying the organic fertilizer in this application;

[0032] Figure 5 Comparison pictures of pitaya fruits applied with the liquid organic fertilizer of the present application (left) and applied with the fertilizer of Comparative Example 4 (right). DETAILED DESCRIPTION

[0033] In order to make the above-mentioned purpose, features and advantages of the present invention more obvious and easy to understand, the specific embodiments of the present invention are described in detail below. In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without violating the connotation of the present invention, so the present invention is not limited by the specific embodiments disclosed below.

[0034] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those generally understood by those skilled in the art of the present invention. The terms used herein in the specification of the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention. The term "and / or" used herein includes any and all combinations of one or more related listed items.

[0035] The present invention provides a process for preparing liquid organic fertilizer, comprising the following steps:

[0036] Step S1: collecting the animal excrement and transporting it to a solid-liquid separator for solid-liquid separation to separate urine and feces;

[0037] The manure residue can be processed as follows to improve the efficient utilization of animal excrement. The manure residue is added to auxiliary materials and a specific composite microbial enzyme preparation for anaerobic fermentation biological treatment; wherein, during the anaerobic fermentation biological treatment process, the temperature is controlled at 60-75°C, the pile is turned once every 2 days, and the fermentation time is 15 days, so that solid organic fertilizer is obtained.

[0038] The composite microbial enzyme preparation includes the fermentation bacteria and the functional fermentation bacteria used in the present invention, and some other elements including but not limited to amino acids, trace elements, etc. are added to prepare the biological preparation.

[0039] Under the action of compound microbial enzyme preparation, the temperature rises quickly, the fermentation efficiency is improved, and the fermentation cycle is shortened. The temperature of the compost turning is monitored in real time and controlled at 60-75℃. Compost turning can increase the oxygen content of the fermentation, prevent compaction, improve heat dissipation, prevent the internal temperature of the compost pile from being too high, causing damage to the microbial flora, and avoid reducing the fermentation effect.

[0040] Step S2: The separated urine is transported to an anaerobic tank, and allowed to stand and ferment in an anaerobic environment. After fermentation in at least two anaerobic tanks for 10 days, the urine is transported to an aerobic tank for aeration fermentation and decomposition. The urine is allowed to stand and ferment in an aerobic and facultative anaerobic environment. After fermentation in at least two aerobic tanks for 10 days, the pre-treated urine of the anaerobic and aerobic two-stage biotechnology is obtained. This step allows the macromolecular organic matter in the urine to be fully degraded.

[0041] Step S3: heating the pre-treated urine, using an evaporator tank with controllable inlet and outlet liquid, controllable temperature, and controllable stirring program for evaporation cycle heating, while performing intermittent stirring, and when the urine temperature rises to 80°C, the evaporator stops heating. When the urine temperature drops to 45°C, thermally degraded urine is obtained;

[0042] The specific indirect stirring procedure is: work for 30 minutes, stop for 10 minutes intermittently, and repeat this process. After the full degradation of this step, the E. coli, roundworm eggs and other harmful bacteria and heavy metals in the urine are eliminated, and the macromolecular organic matter is fully degraded again and converted into small molecule soluble organic matter.

[0043] Step S4: the thermally degradable urine is kept at a constant temperature of 45-55° C., fermentation bacteria are added, the fermentation bacteria and the thermally degradable urine are fully mixed, and high-speed stirring is continued for 3 days to obtain fermented and degraded urine; through high-speed stirring and sufficient aerobic decomposition process, the macromolecular organic matter in the urine is further decomposed, and it is also beneficial to remove the unpleasant taste such as hydrogen sulfide contained in the urine.

[0044] Step S5: Under an anaerobic environment, the fermented and degraded urine is kept at a constant temperature of 45-55° C., functional bacteria are added, and the mixture is fully stirred for 24 hours. At this time, the urine has been efficiently converted into biological organic fertilizer within 24 hours. Then, carbon element microbial raw materials are added under an anaerobic state to mix the elements, and the mixture is stirred evenly to promote microbial reproduction, increase the fertilizer efficiency and biological activity of the biological organic fertilizer, achieve the effect of dissolving potassium and phosphorus, and obtain anaerobic fermented urine;

[0045] Step S6: In order to ensure that the microorganisms in the organic fertilizer are in a biologically active state, the anaerobic fermented urine is placed in a semi-anaerobic and facultative aerobic environment, and composite deodorizing bacteria are added to remove the mixed odor of ammonia and nitrogen produced in the process. After sufficient stirring, liquid organic fertilizer is obtained.

[0046] Preferably, the fermentation bacteria is a mixture of microorganisms such as Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, Streptomyces rocheri, Aspergillus oryzae, Bacillus amyloliquefaciens, lactic acid bacteria, phosphate-soluble bacteria, Trichoderma viride, and Bacillus stearothermophilus licheniformis.

[0047] Preferably, among the fermentation bacteria, the mass ratio of Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, Streptomyces rocheri, Aspergillus oryzae, Bacillus amyloliquefaciens, lactic acid bacteria, phosphate-soluble bacteria, Trichoderma viride, and Bacillus stearothermophilus licheniformis is 20-30:5-10:1-5:10-15:5-10:10-15:1-5:1-5:1-5:1-5:1-5.

[0048] Specifically, the mass ratio of combination 1 Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, Streptomyces rocheri, Aspergillus oryzae, Bacillus amyloliquefaciens, lactic acid bacteria, phosphate-soluble bacteria, Trichoderma viride, and Bacillus stearolicheniformis is 20:5:1:10:5:10:1:5:1:5, and the mass ratio of combination 2 Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, Streptomyces rocheri, Aspergillus oryzae, Bacillus amyloliquefaciens, lactic acid bacteria, phosphate-soluble bacteria, Trichoderma viride, and Bacillus stearothermophilus is 20:5:1:10:5:10:1:5:1:5. The mass ratio of bacteria, phosphate-soluble bacteria, Trichoderma viride, and thermophilic Bacillus stearolicheniformis is 30:10:5:15:10:15:5:1:5:1, and the mass ratio of combination 3 of Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, Streptomyces rocheri, Aspergillus oryzae, Bacillus amyloliquefaciens, lactic acid bacteria, phosphate-soluble bacteria, Trichoderma viride, and thermophilic Bacillus stearolicheniformis is 25:8:3:12:8:13:3:2:2:3.

[0049] In a preferred embodiment, the fermentation bacteria are composed of Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, Streptomyces rocheri, Aspergillus oryzae, Bacillus amyloliquefaciens, lactic acid bacteria, phosphate-soluble bacteria, Trichoderma viride, and Bacillus stearothermophilus licheniformis in a mass ratio of 25:10:5:15:10:15:1:5:5:5.

[0050] Preferably, the functional bacteria is a mixture of microorganisms such as Bacillus subtilis, Brevibacillus laterosporus, Bacillus amyloliquefaciens, Bacillus licheniformis and Bacillus gelatinus.

[0051] Preferably, in the functional bacteria, the mass ratio of Bacillus subtilis, Brevibacillus laterosporus, Bacillus amyloliquefaciens, Bacillus licheniformis and Bacillus gelatinosa is 30-40:10-20:10-20:10-20:10-20.

[0052] Specifically, the mass ratio of combination 1 Bacillus subtilis, Brevibacillus laterosporus, Bacillus amyloliquefaciens, Bacillus licheniformis and Bacillus gelatinosa is 30:10:20:20:10; the mass ratio of combination 2 Bacillus subtilis, Brevibacillus laterosporus, Bacillus amyloliquefaciens, Bacillus licheniformis and Bacillus gelatinosa is 40:20:10:10:15; the mass ratio of combination 3 Bacillus subtilis, Brevibacillus laterosporus, Bacillus amyloliquefaciens, Bacillus licheniformis and Bacillus gelatinosa is 35:12:15:18:20.

[0053] In a preferred embodiment, the functional bacteria are composed of Bacillus subtilis, Brevibacillus laterosporus, Bacillus amyloliquefaciens, Bacillus licheniformis and Bacillus gelatinus in a mass ratio of 35:15:15:18:15.

[0054] Preferably, the carbon element microbial raw material includes mineral source potassium fulvic acid, humic acid and sugar rice flour, and the mass ratio of the mineral source potassium fulvic acid, humic acid and sugar rice flour is 1-10:10-20:10-15.

[0055] Specifically, the mass ratio of mineral source potassium humate, humic acid and sugar rice flour in combination 1 is 1:10:15, the mass ratio of mineral source potassium humate, humic acid and sugar rice flour in combination 2 is 10:20:10, and the mass ratio of mineral source potassium humate, humic acid and sugar rice flour in combination 3 is 10:15:13.

[0056] In a preferred embodiment, the carbon element microbial raw material is composed of mineral potassium fulvic acid, humic acid and sugar rice flour in a mass ratio of 10:10:15.

[0057] Preferably, the composite deodorizing bacteria includes Bacillus subtilis, Lactobacillus plantarum, photosynthetic bacteria, Bacillus licheniformis, yeast and Enterococcus faecalis, and the mass ratio of Bacillus subtilis, Lactobacillus plantarum, photosynthetic bacteria, Bacillus licheniformis, yeast and Enterococcus faecalis is 1-5:30-40:1-5:1-5:1-5:1-5. The specific ratio can be: 1:30:1:1:1:1, 5:40:5:1:5:5.

[0058] In a preferred embodiment, the composite deodorizing bacteria is composed of Bacillus subtilis, Lactobacillus plantarum, photosynthetic bacteria, Bacillus licheniformis, yeast and Enterococcus faecalis in a mass ratio of 5:35:5:1:5:5.

[0059] Preferably, the added amount of the fermentation bacteria is 0.03-0.04% of the total amount of urine after separation in step S1, and specifically can be 0.003%, 0.0035%, or 0.004%.

[0060] The added amount of the functional bacteria is 0.10-0.15% of the total amount of urine after separation in step S1, and specifically can be 0.10%, 0.11%, 0.12%, 0.13%, 0.14%, 0.15%.

[0061] The amount of carbon element microbial raw material added is 0.10-0.12% of the total amount of urine after separation in step S1, and can be specifically 0.10%, 0.11%, or 0.12%.

[0062] The added amount of the composite deodorizing bacteria is 0.18-0.22% of the total amount of urine after separation in step S1, and specifically can be 0.18%, 0.19%, 0.20%, 0.21%, 0.22%.

[0063] Another object of the present invention is to provide an application of liquid organic fertilizer in improving farmland and soil, wherein the liquid organic fertilizer can be diluted 2-15 times for application according to different crops.

[0064] The following explains:

[0065] Fertilization instructions for vegetables

[0066] For example, nightshade vegetables, melon vegetables, cabbage, perennial vegetables, etc.:

[0067] Transplanting period: liquid organic fertilizer needs to be diluted 9 times; seedling stage: liquid organic fertilizer needs to be diluted 8 times; growth and maturity stage: liquid organic fertilizer needs to be diluted 6 times.

[0068] For example, green leafy vegetables:

[0069] Sowing and germination: liquid organic fertilizer needs to be diluted 12 times; seedling stage: liquid organic fertilizer needs to be diluted 8 times; growth and maturity: liquid organic fertilizer needs to be diluted 6 times.

[0070] Fertilization instructions for fruit trees

[0071] For example, citrus, navel orange, plum, grapefruit, kiwi, peach, dragon fruit, mango, lychee, longan, etc.:

[0072] Seedling stage: dilute liquid organic fertilizer 6 times; fruit trees over 3 years old: dilute liquid organic fertilizer 4 times; for example, grapes

[0073] 1-3 years old: liquid organic fertilizer needs to be diluted 7 times; fruit trees over 3 years old: liquid organic fertilizer needs to be diluted 3-5 times.

[0074] Fertilization instructions for other crops:

[0075] Tobacco: Seedbed stage: liquid organic fertilizer needs to be diluted 12 times; transplanting stage: liquid organic fertilizer needs to be diluted 8 times; growth stage: liquid organic fertilizer needs to be diluted 6 times.

[0076] For example, landscaping

[0077] Seedling stage: liquid organic fertilizer needs to be diluted 8 times; seedling stage: liquid organic fertilizer needs to be diluted 6 times.

[0078] The liquid organic fertilizer of the present invention is suitable for crops such as rice, wheat, corn, cereals, potato crops, bean crops, rapeseed, peanuts, sunflowers and other oil crops, cotton, sugar crops, hemp crops, eggplant vegetables, melon vegetables, root vegetables, potato vegetables, cabbage vegetables, kale vegetables, leafy vegetables, bean vegetables, onion and garlic vegetables, perennial vegetables, aquatic vegetables, citrus fruits, melons and fruits, berries, drupes, pome fruits, nuts, tropical fruits, ornamental flowers, ornamental foliage flowers, ornamental fruit flowers, ornamental stem flowers, ornamental bud flowers, woody Chinese medicinal materials, herbal Chinese medicinal materials, shade-loving Chinese medicinal materials, tea and tobacco, other hobby crops, gardening crops and the like, and has a wide range of applications.

[0079] Example 1

[0080] The present invention is further described below by taking pig excrement as a specific example.

[0081] Prepare fermentation bacteria: According to the mass ratio of 25:10:5:15:10:15:1:5:5:5, weigh Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, Streptomyces rocheri, Aspergillus oryzae, Bacillus amyloliquefaciens, lactic acid bacteria, phosphate-soluble bacteria, Trichoderma viride, Bacillus stearothermophilus licheniformis and other microorganisms respectively, stir until mixed evenly, and then prepare fermentation bacteria.

[0082] Preparation of functional bacteria: According to the mass ratio of 35:15:15:18:15, Bacillus subtilis, Brevibacillus laterosporus, Bacillus amyloliquefaciens, Bacillus licheniformis and Bacillus mucilaginosus are weighed respectively, and the mixture is stirred until uniformly mixed to obtain the functional bacteria.

[0083] Preparation of carbon element microbial raw materials: according to the mass ratio of 10:10:15, weigh mineral source potassium fulvic acid, humic acid and sugar rice flour and other raw materials, mix them evenly, and then prepare the carbon element microbial raw materials.

[0084] Preparation of composite deodorizing bacteria: According to the mass ratio of 5:35:5:1:5:5, weigh Bacillus subtilis, Lactobacillus plantarum, photosynthetic bacteria, Bacillus licheniformis, yeast, Enterococcus faecalis and other microorganisms, mix them evenly, and then prepare the composite deodorizing bacteria.

[0085] The composite microbial enzyme preparation includes the fermentation bacteria and the functional fermentation bacteria used in the present invention, and some other elements (including but not limited to amino acids, trace elements) are added to prepare the biological preparation.

[0086] Based on the total mass of urine after separation being 100 tons, the amount of fermentation bacteria added is 35kg. The amount of functional bacteria added is 150kg. Among the carbon element microbial raw materials, there are 800kg of mineral potassium fulvic acid, 100kg of humic acid, and 200kg of sugar rice powder. The amount of compound deodorizing bacteria added is 200kg.

[0087] The following is the preparation process of liquid organic fertilizer.

[0088] The present invention provides a process for preparing liquid organic fertilizer, comprising the following steps:

[0089] Step S1: collecting the animal excrement and transporting it to a solid-liquid separator for solid-liquid separation to separate urine and feces;

[0090] The manure residue is treated as follows to improve the efficient utilization of animal excrement. The manure residue is added to auxiliary materials and a specific composite microbial enzyme preparation for anaerobic fermentation biological treatment; wherein, during the anaerobic fermentation biological treatment process, the temperature is controlled at 60-75°C, the pile is turned once every 2 days, and the fermentation time is 15 days, so that solid organic fertilizer is obtained.

[0091] Under the action of compound microbial enzyme preparation, the temperature rises quickly, the fermentation efficiency is improved, and the fermentation cycle is shortened. The temperature of the compost turning is monitored in real time and controlled at 60-75℃. Compost turning can increase the oxygen content of the fermentation, prevent compaction, improve heat dissipation, prevent the internal temperature of the compost pile from being too high, causing damage to the microbial flora, and avoid reducing the fermentation effect.

[0092] Step S2: The separated urine is transported to an anaerobic tank, and allowed to ferment in an anaerobic environment. After fermentation in four anaerobic tanks for 10 days, the urine is transported to an aerobic tank for aeration fermentation and decomposition. The urine is allowed to ferment in an aerobic and facultative anaerobic environment. After fermentation in two aerobic tanks for 10 days, the pre-treated urine of the anaerobic and aerobic two-stage biotechnology is obtained. This step allows the macromolecular organic matter in the urine to be fully degraded.

[0093] Step S3: heating the pre-treated urine, using an evaporator tank with controllable inlet and outlet liquid, controllable temperature, and controllable stirring program for evaporation cycle heating, while performing intermittent stirring, and when the urine temperature rises to 80°C, the evaporator stops heating. When the urine temperature drops to 45°C, thermally degraded urine is obtained;

[0094] The specific indirect stirring procedure is: work for 30 minutes, stop for 10 minutes intermittently, and repeat this process. After the full degradation of this step, the E. coli, roundworm eggs and other harmful bacteria and heavy metals in the urine are eliminated, and the macromolecular organic matter is fully degraded again and converted into small molecule soluble organic matter.

[0095] Step S4: the thermally degradable urine is kept at a constant temperature of 45-55° C., fermentation bacteria are added, the fermentation bacteria and the thermally degradable urine are fully mixed, and high-speed stirring is continued for 3 days to obtain fermented and degraded urine; through high-speed stirring and sufficient aerobic decomposition process, the macromolecular organic matter in the urine is further decomposed, and it is also beneficial to remove the unpleasant taste such as hydrogen sulfide contained in the urine.

[0096] Step S5: Under an anaerobic environment, the fermented and degraded urine is kept at a constant temperature of 45-55° C., functional bacteria are added, and the mixture is fully stirred for 24 hours. At this time, the urine has been efficiently converted into biological organic fertilizer within 24 hours. Then, carbon element microbial raw materials are added under an anaerobic state to mix the elements, and the mixture is stirred evenly to promote microbial reproduction, increase the fertilizer efficiency and biological activity of the biological organic fertilizer, achieve the effect of dissolving potassium and phosphorus, and obtain anaerobic fermented urine;

[0097] Step S6: To ensure that the microorganisms in the organic fertilizer are in a biologically active state, the anaerobic fermented urine is placed in a semi-anaerobic and facultative anaerobic environment, and composite deodorizing bacteria are added to remove the mixed odor of ammonia and nitrogen produced in the process. After sufficient stirring, liquid organic fertilizer is obtained.

[0098] The following liquid organic fertilizer prepared in Example 1 was tested for fecal coliform count and ascaris egg mortality according to GB / T 19524.2-2004, and total nutrients according to NY / T 1977-2010. The judgment was based on NY / T798-2015 "Compound Microbial Fertilizer". The test data are shown in Table 1:

[0099] Table 1 Test results of fecal coliform count and ascaris egg mortality in liquid organic fertilizer

[0100]

[0101] The liquid organic fertilizer prepared in Example 1 is qualified in both indicators. The fecal coliform count is an indicator used to evaluate the degree of fecal contamination in the fertilizer, and the parasite egg mortality rate is used to evaluate the killing effect of parasite eggs in the fertilizer. The qualified results of these two indicators indicate that the liquid organic fertilizer prepared in Example 1 has reached a certain hygiene standard.

[0102] The liquid organic fertilizer prepared by embodiment 1 reaches 15 in nitrogen, phosphorus and potassium (NPK) total nutrient content, which significantly exceeds the minimum standard (≥5) stipulated by the state, is 3 times of the national standard, and can provide more abundant nutrition for crops. And because the nutrient content of the present invention is high, the organic fertilizer of high nutrition can provide lasting and stable nutrient release for crops, avoids frequent fertilization, and saves labor cost. After application, the liquid organic fertilizer of the present invention can significantly improve the growth rate and yield of crops (further demonstrated by the following application examples).

[0103] The liquid organic fertilizer prepared in Example 1 was tested according to NY / T 798-2015 "Compound Microbial Fertilizer". The test data are as follows:

[0104] Effective viable count (cfu): 3.7631 billion / mL, meeting the requirement of ≥50 million / mL specified in NY / T 2321-2013 standard;

[0105] Miscellaneous bacteria rate: 0.06%, in line with the requirement of ≤15% specified in NY / T 2321-2013 standard.

[0106] At the same time, according to the testing method of NY / T 2321-2013 standard, the following were detected:

[0107] Bacillus licheniformis count (cfu): 0.001 billion / mL;

[0108] Bacillus subtilis count (cfu): 96.3 million / mL;

[0109] Lactobacillus plantarum count (cfu): 3.6667 billion / mL;

[0110] In summary, the effective viable count and the miscellaneous bacteria rate of the liquid organic fertilizer prepared by embodiment 1 all meet the requirement of NY / T2321-2013 standard, and simultaneously detect the number of bacillus licheniformis, the number of bacillus subtilis and the number of lactobacillus plantarum, contain rich organic matter, perform well in terms of microbial activity, be conducive to improving soil quality and promoting microbial diversity, and be environmentally friendly. And the liquid organic fertilizer is placed at room temperature for more than half a year, and the liquid organic fertilizer is stable in shape for more than one year, does not deteriorate, and the colony number change rate is within 1%, and still has very high fertility.

[0111] The liquid organic fertilizer prepared in Example 1 was tested for heavy metal total cadmium, total chromium, total mercury, total lead and total arsenic according to GB 38400-2019 "Limit Requirements for Toxic and Hazardous Substances in Fertilizers". The test data are shown in Table 2:

[0112] Table 2 Detection results of heavy metals in liquid organic fertilizer

[0113] Serial number Project Name Test results Detection limit / technical requirements in conclusion 1 Total Cadmium Not detected 0.4 / ≤3 conform to 2 Total Chromium Not detected 0.1 / ≤150 conform to 3 Total Mercury Not detected 0.1 / ≤2 conform to 4 Total Lead Not detected 1 / ≤50 conform to 5 Total Arsenic 0.2mg / kg 0.1 / ≤15 conform to

[0114] The liquid organic fertilizer prepared by the invention does not contain harmful heavy metal components, does not cause pollution to the soil and the surrounding environment, and is helpful to maintain ecological balance; it meets the requirements of sustainable agricultural development, is conducive to the recycling of resources and the long-term stability of the agricultural ecosystem.

[0115] The use of the liquid organic fertilizer of the present invention can reduce the risk of crops absorbing harmful substances, ensure the healthy growth of crops, produce higher quality agricultural products, and meet the market demand for pollution-free and green food; at the same time, it can indirectly ensure food safety and reduce potential threats to human health.

[0116] In summary, the liquid organic fertilizer prepared by the present invention has passed a number of quality tests, showing its safety and high nutritional value, and is a high-quality organic fertilizer.

[0117] Application Example 1:

[0118] Nicotiana vulgaris was planted on the same plot at the same time, and the liquid organic fertilizer prepared in the example and the commercially available organic fertilizer (biological organic fertilizer purchased from Zhong*Gaoke, comparative example 1) were applied respectively.

[0119] Yellow tobacco experiment address: Zhang*Ming, a yellow tobacco grower in Sanjiao Village, Hukou Town, Nanxiong City. Area: 3 mu.

[0120] The liquid organic fertilizer prepared in the embodiment and the commercially available organic fertilizer fertilization method are as follows: in the transplanting (seedling) stage, the first rooting water is applied, and the fertilizers in the embodiment and the comparative example are diluted 12 times; the second topdressing is about 10 days later, when the film is removed, the second fertilizer is applied, and the fertilizers in the embodiment and the comparative example are diluted 8 times; the third topdressing is about one month later, and the fertilizers in the embodiment and the comparative example in the seedling stage are diluted 6 times; the fourth topdressing is about two months later, and the fertilizers in the embodiment and the comparative example in the growth stage are diluted 6 times; a total of four fertilizations.

[0121] After 120 days, the agronomic traits of yellow tobacco were observed and measured. Data collection personnel: the applicant, Guangdong Tobacco Shaoguan Co., Ltd. Nanxiong Branch, obtained the following data, see Table 3 for details:

[0122] Table 3 Observation and measurement data of agronomic traits of yellow tobacco

[0123]

[0124] In addition to the above test data, please refer to the appendix of the manual. Figure 1 , on the left is the liquid organic fertilizer of this application, and a comparison picture of the leaves of Nicotiana candida applied with other organic fertilizers (right). After applying the liquid organic fertilizer of this application, the leaf length, leaf width and plant robustness of Nicotiana candida are better than those applied with other organic fertilizers.

[0125] Furthermore, when the liquid organic fertilizer of the present application was used to fertilize yellow tobacco, no insect pests were found, while when other organic fertilizers were used to fertilize yellow tobacco, aphids, planthoppers and other pests were found to suck the juice from the leaves.

[0126] Application Example 2

[0127] Rice was planted at the same time and in the same plot, and the liquid organic fertilizer prepared in the embodiment, the commercially available compound fertilizer (compound fertilizer purchased from Nanjing * Hua Yongda Industry, Comparative Example 2) and the mixed fertilizer prepared by mixing the liquid organic fertilizer in the embodiment with the commercially available compound fertilizer in a mass ratio of 7:3 (Comparative Example 3) were applied respectively.

[0128] The rice experiment was conducted at Furong Village, Nanmu Town, Nanxiong City. Three sets of data were collected for each scheme, and the area of ​​each collection point was 1.11 square meters.

[0129] The liquid organic fertilizer fertilization method of this embodiment is as follows: the rice field is first soaked in water for 3 days, and then the organic fertilizer is evenly poured in without dilution. After waiting for 3 days, the field is evenly tilled by a rotary tiller, and the organic fertilizer is fully dissolved in the soil, and the whole fertilization process is completed at one time.

[0130] The fertilization method of the commercially available compound fertilizer (Comparative Example 2) and the mixed fertilizer prepared by mixing the liquid organic fertilizer of the embodiment with the commercially available compound fertilizer in a mass ratio of 7:3 is the same as above.

[0131] The rice matured and was harvested in about 120 days. The growth characteristics of the rice were observed and measured. The data were collected by the Nanxiong Agricultural Bureau and the applicant. The following data were obtained, as shown in Table 4:

[0132] Table 4 Observation and measurement data of rice growth traits

[0133]

[0134] In addition to the above test data, please refer to the appendix of the manual. Figure 2 The picture shows the actual situation of fertilization. The left side shows the application of liquid organic fertilizer, and the right side shows the application of other conventional fertilizers. It can be clearly seen that the rice planted on the left is lush, has thick roots and stems, and is free of insect pests. Its growth trend is better than that of the rice planted on the right.

[0135] In conjunction with the instruction manual Figure 3 and attached Figure 4 , randomly selected growing rice plants for growth comparison, attached Figure 3 The growth of rice after conventional fertilizer application in comparative example 2 is shown in Figure 2. The root length is 16 cm, the seedling height is 42 cm, and the number of split plants is 5. Figure 4 This is the growth condition of rice after applying the liquid organic fertilizer of this application, root length: 20cm, seedling height: 49cm, number of split plants: 9 plants.

[0136] Application Example 3

[0137] At the same time, the liquid organic fertilizer prepared in the embodiment and the commercially available compound fertilizer (purchased from Shi*li pitaya special fertilizer, comparative example 4) were applied to the Miaoling 2-year variety Dahong series pitaya trees respectively.

[0138] Dragon fruit test address: Jinxiong Agriculture, Xiongzhou Street, Nanxiong City.

[0139] The liquid organic fertilizer and commercially available compound fertilizer fertilization method of the present embodiment are as follows: when pitaya is in bloom, the first fertilization is performed with the organic fertilizer diluted 6 times, 100 catties per mu; the second time, when the fruit is swelling, the organic fertilizer is diluted 4 times, 120 catties per mu; the third and fourth times are the same as above, with fertilization applied once every month.

[0140] Dragon fruit can be produced after about 120 days. The produced dragon fruit is observed and measured. Data collection personnel: Nanxiong Agricultural Bureau, applicant, and the following data are obtained:

[0141] See attached Figure 5 , attached Figure 5 The left side in the middle is the pitaya produced by using comparative example 4, while the right side is the pitaya produced by using the embodiment of the present application. It is obvious that the pitaya on the right is twice as large as that on the left.

[0142] In terms of weight data, the pitaya on the left weighs 650g, and the pitaya on the right weighs 900g. In terms of taste and sweetness, the pitaya on the right that has been fertilized with the liquid organic fertilizer of the present application has a better taste and is sweeter.

[0143] In summary, the liquid organic fertilizer prepared by the present invention realizes the resource utilization and circular economic utilization of agricultural wastes through the efficient conversion of pig excrement, thereby reducing the risk of environmental pollution.

[0144] Liquid organic fertilizer of the present invention is applicable to various crops, including cereals, vegetables, fruits, tea, flowers and aquatic plants, etc., and through multiple groups of planting experiments, it is proved that the application of liquid organic fertilizer of the present invention can significantly promote plant growth, which is manifested in that the plant is more robust, the branches and leaves are luxuriant, and the fruit size increases. The crops using organic fertilizer of the present invention simultaneously show less occurrence of diseases and insect pests, which may be attributed to the fact that organic fertilizer improves the soil microbial environment and enhances the disease resistance of the plant itself. Due to its natural composition and abundant nutrition supply, the agricultural products using organic fertilizer of the present invention often have better taste and nutritional value. Compared with traditional chemical fertilizers, using organic fertilizer of the present invention can reduce the usage of chemical fertilizers, and reduce the dependence of agricultural production on chemical input products.

[0145] The technical features of the above-described embodiments may be arbitrarily combined. To make the description concise, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0146] The above-mentioned embodiments only express several implementation methods of the present invention, and the description is relatively specific and detailed, but it cannot be understood as limiting the scope of the invention patent. It should be pointed out that for ordinary technicians in this field, several modifications and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be based on the attached claims, and the description can be used to interpret the content of the claims.

Claims

1. A preparation process for liquid organic fertilizer, characterized in that: The following steps are involved: Step S1: collecting animal excrement and performing solid-liquid separation to separate urine and feces; Step S2: the separated urine is placed in an anaerobic environment for 10 days for static fermentation, and then placed in an aerobic and facultative anaerobic environment for static aeration fermentation for 10 days to obtain pre-treated urine; Step S3: heating the pre-treated urine while stirring intermittently, and stopping heating when the urine temperature rises to 80° C., and obtaining thermally degraded urine when the urine temperature drops to 45° C.; Step S4: the heat-degradable urine is kept at a constant temperature of 45-55° C., fermentation bacteria are added, the fermentation bacteria and the heat-degradable urine are fully mixed, and high-speed stirring is continued for 3 days to obtain fermented degraded urine; Step S5: Under an anaerobic environment, the fermented degraded urine is placed at a constant temperature of 20-60° C., functional bacteria are added, and after being fully stirred for 24 hours, carbon element microbial raw materials are added under an anaerobic state to perform element blending, and the mixture is stirred evenly to obtain anaerobic fermented urine; Step S6: placing the anaerobic fermented urine in a semi-anaerobic and facultative aerobic environment, adding composite deodorizing bacteria, and fully stirring to obtain liquid organic fertilizer; Among them, the added amount of the fermentation bacteria is 0.03-0.04% of the total amount of urine after separation in step S1, the added amount of the functional bacteria is 0.10-0.15% of the total amount of urine after separation in step S1, the added amount of the carbon element microbial raw material is 0.10-0.12% of the total amount of urine after separation in step S1, and the added amount of the composite deodorizing bacteria is 0.18-0.22% of the total amount of urine after separation in step S1.

2. A preparation technology for liquid organic fertilizer according to claim 1, characterized in that: The fermentation bacteria is a mixture of Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, Streptomyces rocheri, Aspergillus oryzae, Bacillus amyloliquefaciens, lactic acid bacteria, phosphate-soluble bacteria, Trichoderma viride and Bacillus stearothermophilus licheniformis.

3. A preparation technology for liquid organic fertilizer according to claim 2, characterized in that: Among the fermentation bacteria, the mass ratio of Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, Streptomyces rocheri, Aspergillus oryzae, Bacillus amyloliquefaciens, lactic acid bacteria, phosphate-soluble bacteria, Trichoderma viride and Bacillus stearothermophilus licheniformis is 20-30:5-10:1-5:10-15:5-10:10-15:1-5:1-5:1-5:1-5:1-5.

4. A preparation technology for liquid organic fertilizer according to claim 1, characterized in that: The functional bacteria are a mixture of Bacillus subtilis, Bacillus laterosporus, Bacillus amyloliquefaciens, Bacillus licheniformis and Bacillus gelatinus.

5. A preparation technology for liquid organic fertilizer according to claim 4, characterized in that: In the functional bacteria, the mass ratio of Bacillus subtilis, Brevibacillus laterosporus, Bacillus amyloliquefaciens, Bacillus licheniformis and Bacillus gelatinosa is 30-40:10-20:10-20:10-20:10-20.

6. A preparation technology for liquid organic fertilizer according to claim 1, characterized in that: The carbon element microbial raw material comprises mineral source potassium fulvic acid, humic acid and sugar rice flour, and the mass ratio of the mineral source potassium fulvic acid, humic acid and sugar rice flour is 1-10:10-20:10-15.

7. A preparation technology for liquid organic fertilizer according to claim 1, characterized in that: The composite deodorizing bacteria comprises bacillus subtilis, lactobacillus plantarum, photosynthetic bacteria, bacillus licheniformis, yeast and enterococcus faecalis, and the mass ratio of the bacillus subtilis, lactobacillus plantarum, photosynthetic bacteria, bacillus licheniformis, yeast and enterococcus faecalis is 1-5:30-40:1-5:1-5:1-5:1-5.

8. A preparation technology for liquid organic fertilizer according to claim 1, characterized in that: In step S1, the feces are treated as follows: the feces are added with auxiliary materials and a specific composite microbial enzyme preparation for anaerobic fermentation biological treatment; wherein, during the anaerobic fermentation biological treatment process, the temperature is controlled at 50-75°C, the pile is turned once every 2 days, and the fermentation time is 15 days, so as to obtain solid organic fertilizer.

9. Use of the liquid organic fertilizer obtained by the preparation process according to any one of claims 1 to 8 in improving farmland and soil.

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