A kind of additive composition and its application

Abstract

The present invention provides an additive composition and its application, wherein the additive composition comprises 10-15 parts of Chinese herbal medicine, 3-5 parts of norfloxacin, and 1.5-3 parts of vitamin powder by weight, and the Chinese herbal medicine formula is as follows: roasted astragalus, codonopsis, atractylodes, angelica, tangerine peel, roasted licorice, cimicifuga, bupleurum, jujube, tuckahoe, orientalis, coptis root, rhubarb, and the mass ratio is 3:2:2:2:2:1.5:1:1:1:1:1:1:1:1. The additive composition provided by the present invention can be effectively used for the prevention and treatment of Chinese sturgeon gill swelling disease, and plays an important role in protecting the Chinese sturgeon species.

Description

Technical Field

[0001] The invention relates to an additive composition and application thereof, belonging to the field of biotechnology. Background Art

[0002] The Chinese sturgeon is a national first-class protected aquatic wild animal. Carrying out the protection of the Chinese sturgeon species is particularly important for the restoration of natural resources. In the process of ex situ protection of the Chinese sturgeon, fish disease prevention and control is an important link that affects the survival rate of sturgeons. In recent years, gill swelling disease is a new disease in the breeding process of Chinese sturgeon juveniles. The disease has the characteristics of rapid onset, strong infectiousness, and short course of disease, which has a certain impact on the survival rate of Chinese sturgeon breeding. However, at present, conventional drugs and methods of use are not effective in preventing and treating this disease.

[0003] Based on this, how to improve the survival rate of Chinese sturgeon during breeding has become an urgent problem to be solved in this field. Summary of the invention

[0004] The invention provides an additive composition. After being used in Chinese sturgeon breeding, the additive composition can significantly improve the immunity of Chinese sturgeon and has a good preventive and therapeutic effect on Chinese sturgeon gill swelling disease.

[0005] The present invention also provides a feed, which comprises the additive composition, thereby being able to significantly improve the cure rate of Chinese sturgeon gill swelling disease and improve the survival rate of Chinese sturgeon farming.

[0006] The present invention also provides an application of the additive composition as a medicine for preparing a medicine for treating Chinese sturgeon gill swelling. In the process of preparing the medicine for treating Chinese sturgeon gill swelling, the additive composition is used, which has a significant effect on treating Chinese sturgeon gill swelling.

[0007] The present invention also provides a method for feeding Chinese sturgeon. The feed is used in the feeding process, so the cure rate of Chinese sturgeon gill swelling disease can be significantly improved, and the survival rate in the breeding process of Chinese sturgeon can be improved.

[0008] The present invention also provides a pharmaceutical composition, which comprises the above-mentioned additive composition, so the pharmaceutical composition can effectively treat Chinese sturgeon gill swelling disease and promote large-scale breeding of Chinese sturgeon.

[0009] The present invention provides an additive composition, which comprises 10-15 parts of Chinese herbal medicine, 3-5 parts of norfloxacin, and 1.5-3 parts of vitamin powder by weight. The Chinese herbal medicine formula is as follows: roasted astragalus, codonopsis, atractylodes, angelica, tangerine peel, roasted liquorice, cimicifuga, bupleurum, jujube, tuckahoe, oriental water plantain, coptis root, and rhubarb, with a mass ratio of 3:2:2:2:2:1.5:1:1:1:1:1:1:1:1.

[0010] In the additive composition as described above, the weight portion of the Chinese herbal medicine is 15 parts, the weight portion of norfloxacin is 4 parts, and the weight portion of the vitamin additive powder is 2 parts.

[0011] In another aspect, the present invention provides a feed comprising the additive composition as described above.

[0012] The feed as described above also includes conventional feed, and the mass ratio of the additive composition to the conventional feed is 1.5:100.

[0013] In another aspect, the present invention provides a use of the additive composition as described above in preparing a medicament for treating Chinese sturgeon gill swelling.

[0014] In another aspect, the present invention provides a method for feeding Chinese sturgeon, which comprises feeding Chinese sturgeon with the feed as described above.

[0015] In the feeding method as described above, the daily feeding amount during the feeding process is 1.0-1.2% of the body weight, the feeding frequency is 3 times / day, and the feeding interval is 5-6 hours.

[0016] In the feeding method described above, during the feeding process, the dissolved oxygen content of the aquaculture water is higher than 7.5 mg / L.

[0017] In another aspect, the present invention provides a pharmaceutical composition comprising the additive composition as described above.

[0018] The pharmaceutical composition as described above further comprises auxiliary agents, wherein the auxiliary agents comprise 5-30 parts of doxycycline hydrochloride and 5000-10000 parts of salt in parts by weight.

[0019] The additive composition provided by the present invention is composed of raw materials of specific components. Under the synergistic effect of the raw materials of the specific components, it can effectively improve the immunity of the Chinese sturgeon, has no stress response and no drug resistance, effectively prevents and controls the gill swelling disease of the Chinese sturgeon, and plays an important role in protecting the Chinese sturgeon species.

[0020] The feed provided by the present invention comprises the additive composition, and thus can significantly improve the cure rate of Chinese sturgeon gill swelling disease and improve the survival rate of Chinese sturgeon farming.

[0021] The additive composition provided by the present invention is used as an application in preparing a drug for treating Chinese sturgeon gill swelling. In the process of preparing the drug for treating Chinese sturgeon gill swelling, the additive composition is used, which has a significant effect on treating Chinese sturgeon gill swelling.

[0022] The feeding method of Chinese sturgeon provided by the present invention uses the above-mentioned feed in the feeding process, so the cure rate of Chinese sturgeon gill swelling disease can be significantly improved, and the survival rate in the breeding process of Chinese sturgeon can be improved.

[0023] The pharmaceutical composition provided by the present invention comprises the above-mentioned additive composition, so the pharmaceutical composition can effectively treat Chinese sturgeon gill swelling disease and promote large-scale breeding of Chinese sturgeon. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 This is a picture of the external characteristics of Chinese sturgeon suffering from gill swelling disease;

[0025] Figure 2 Anatomy diagram of a Chinese sturgeon suffering from gill swelling disease. DETAILED DESCRIPTION

[0026] In order to make the purpose, technical solutions and advantages of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in combination with the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0027] The present invention provides an additive composition and its application, wherein the additive composition comprises 10-15 parts of Chinese herbal medicine, 3-5 parts of norfloxacin, and 1.5-3 parts of vitamin powder by weight. The Chinese herbal medicine formula is as follows: roasted astragalus, codonopsis, atractylodes, angelica, tangerine peel, roasted liquorice, cimicifuga, bupleurum, jujube, tuckahoe, orientalis, coptis root, and rhubarb, and the mass ratio is 3:2:2:2:2:1.5:1:1:1:1:1:1:1:1.

[0028] The additive composition provided by the invention can be used for treating Chinese sturgeon gill swelling disease.

[0029] The present invention does not limit the specific diagnostic method of Chinese sturgeon gill swelling disease, and the common diagnostic methods in the field can be used for diagnosis.

[0030] For example, observe the living conditions of Chinese sturgeons. When Chinese sturgeons float on the upper layer of the water and swim slowly and weakly, the breathing rate of the gills is significantly accelerated, the gill filaments are swollen, and the respiratory system is obviously diseased. Some sick fish have red and swollen mouths and different degrees of congestion on the body surface, such as Figure 1 As shown in the figure, the autopsy of the dead Chinese sturgeon showed congestion in the liver, the outer wall of the swim bladder, and the inner and outer walls of the intestines. Figure 2 shown.

[0031] The weight parts of the Chinese herbal medicine are 10-15 parts. Specifically, the weight parts of the Chinese herbal medicine include but are not limited to 10 parts, 11 parts, 12 parts, 13 parts, 14 parts, 15 parts or a range consisting of any two of them.

[0032] The weight portion of norfloxacin is 3-5 parts. Specifically, the weight portion of norfloxacin includes but is not limited to 3 parts, 3.2 parts, 3.4 parts, 3.6 parts, 3.8 parts, 4.0 parts, 4.2 parts, 4.4 parts, 4.6 parts, 4.8 parts, 5.0 parts or a range consisting of any two of them.

[0033] The weight proportion of the vitamin-added powder is 1.5-3 parts. Specifically, the weight proportion of the vitamin-added powder includes but is not limited to 1.5 parts, 1.6 parts, 1.8 parts, 2.0 parts, 2.2 parts, 2.4 parts, 2.6 parts, 2.8 parts, 3.0 parts or a range consisting of any two of them.

[0034] The vitamin-added powder in the present invention can be selected from common vitamin-added powders in the art, as long as it has the effects of promoting growth, increasing appetite, enhancing disease resistance, reducing stress response, treating fatty liver, and protecting and benefiting the liver.

[0035] In a specific embodiment, the vitamin-added powder includes vitamins (A, D3, E, K3, B1, B2, B6, B12), niacin, calcium pantothenate, folic acid, and biotin, wherein each kilogram of the vitamin-added powder contains 15-16.5g of vitamin A, 0.26-0.28g of vitamin D3, 9.5-10.5g of vitamin E, 4-6g of vitamin K3, 1.5-2.5g of vitamin B1, 13-17g of vitamin B2, 7-9g of vitamin B6, 0.02-0.04g of vitamin B12, 25-30g of niacin, 23-27g of calcium pantothenate, 0.4-0.6g of folic acid, and 0.14-0.18g of biotin.

[0036] The roasted astragalus in the present invention is the main drug; the roasted astragalus is sweet in taste, has the effects of invigorating qi, tonifying the middle, raising yang and consolidating the exterior, and is used to treat qi deficiency, fatigue, poor appetite and loose stools.

[0037] The codonopsis pilosula, atractylodes macrocephala and licorice root in the present invention are ministerial drugs, which assist the monarch drug in invigorating qi and tonifying the middle; the codonopsis pilosula is sweet in taste, and has multiple pharmacological effects such as enhancing body immunity, protecting gastrointestinal mucosa and resisting ulcer, promoting hematopoietic function, regulating blood sugar, delaying aging, etc.; the atractylodes macrocephala is bitter and sweet in taste, and has the effects of invigorating the spleen and invigorating qi, drying dampness and promoting diuresis, and stopping sweating; the licorice root is sweet in taste, and has the effects of invigorating the spleen and harmonizing the stomach, invigorating qi and restoring the pulse.

[0038] The angelica, tangerine peel, Poria, oriental Chinese medicine, coptis root, rhubarb, cimicifuga and bupleurum in the present invention are all adjuvants. Angelica nourishes blood, tangerine peel regulates qi and relieves stagnation, and is used together with qi-invigorating and blood-invigorating drugs. Poria and oriental Chinese medicine can both invigorate and purge, coptis root purges the upper Jiao, and rhubarb purges the lower Jiao, making the invigoration not greasy; cimicifuga and bupleurum are more used to raise yang and lift the sunken, relieve liver qi, and help the monarch and ministerial drugs to enhance the positive energy; angelica is sweet and pungent, and has the effects of invigorating blood and activating blood circulation, moistening the intestines and relieving constipation; tangerine peel is bitter and pungent, and has the effects of regulating qi and strengthening the spleen, drying dampness and resolving phlegm, anti-inflammatory, anti-ulcer, and gallbladder-promoting; Poria is sweet, and has the effects of enhancing immunity, antibacterial and anti-inflammatory, antiviral, anti-oxidation, protecting the liver and promoting diuresis; oriental Chinese medicine is sweet, It has the effects of promoting diuresis and eliminating dampness, purging heat, clearing turbidity and lowering lipids, anti-fatty liver, anti-nephritis activity, and immune regulation; Coptis chinensis is bitter, has antibacterial, antiviral, and antiprotozoal effects, and also has the effect of regulating body functions; Rhubarb is bitter, has the effects of purging and attacking accumulation, clearing away heat and purging fire, cooling blood and detoxifying, and has a strong inhibitory effect on most Gram-positive and Gram-negative bacteria (such as fish-harming myxococci, Aeromonas, etc.), and at the same time has antiviral (such as anti-grass carp hemorrhagic disease virus), immune-enhancing, hemostatic and coagulant effects; Cimicifuga heracleifolia is sweet and spicy, has the effects of dispelling exterior pathogens, raising yang, clearing heat and detoxifying; Bupleurum is bitter, has antiviral, protozoanic effects, and anti-liver damage effects.

[0039] Roasted licorice and jujube can harmonize various medicines and also serve as adjuvants; jujube is sweet in taste and has the effects of nourishing the spleen and stomach, replenishing qi and blood, calming the mind, regulating the nutrient and defensive systems, and harmonizing the properties of medicines.

[0040] Norfloxacin is a long-acting, highly effective, broad-spectrum fluoroquinolone antibiotic. Its toxic side effects are generally mild, and it is not easy to develop drug resistance. There is no cross-resistance between it and other similar drugs.

[0041] The above components are mixed according to the above weight parts to obtain the additive composition of the present invention, and the additive composition has a good effect on the treatment of Chinese sturgeon gill swelling. The inventors believe that the reason may be that the combination of various components in specific weight parts can give full play to the synergistic effect between the components, reduce the inflammatory response of Chinese sturgeon, improve immunity, effectively control infection, improve the overall health of Chinese sturgeon, and improve the cure rate of Chinese sturgeon gill swelling.

[0042] Furthermore, in a specific embodiment of the present invention, the weight portion of Chinese herbal medicine is 15 parts, the weight portion of norfloxacin is 4 parts, and the weight portion of vitamin added powder is 2 parts.

[0043] When the weight proportions of Chinese herbal medicine, norfloxacin and vitamin powder in the additive composition are within the above range, the additive composition prepared has a higher cure rate when used for Chinese sturgeon gill swelling. The reasons may be as follows: (1) Synergistic effect of drug combination: the drug compatibility has reached the optimal combination, and Chinese herbal medicine and vitamins work together to promote growth, increase appetite, strengthen immunity, reduce stress, etc. The combination of Chinese herbal medicine and norfloxacin strengthens the resistance of young fish to pathogenic microorganisms. The combination and synergy of Chinese herbal medicine, norfloxacin and multiple vitamins effectively improves the immunity and disease resistance of young Chinese sturgeons, thereby achieving a good drug prevention and treatment effect. (2) The therapeutic dose of drug combination: in the process of improving immunity and preventing and treating pathogenic microorganisms, the drug dosage reaches the therapeutic safe dosage range and plays a role in strengthening immunity and preventing pathogenic microorganisms. The therapeutic effect is not reduced due to insufficient dosage, and the health of young fish is not adversely affected due to the dosage exceeding the safe dosage range.

[0044] In another aspect, the present invention provides a feed comprising the additive composition as described above.

[0045] Since the feed provided by the present invention comprises the additive composition, when the feed is used to feed Chinese sturgeons infected with gill swelling disease, the immunity of Chinese sturgeons can be significantly improved, and the cure rate of the infected Chinese sturgeons can be improved.

[0046] Furthermore, in a specific embodiment of the present invention, conventional feed is also included, and the mass ratio of the additive composition to the conventional feed is 1.5:100.

[0047] The present invention does not limit the nutritional components of conventional feeds, and the Chinese sturgeon feed commonly used in the art can be selected.

[0048] In a specific embodiment, the conventional feed comprises crude protein ≥44.0wt%, crude fat ≥10.0wt%, crude fiber ≤5.0wt%, crude ash ≤16.0wt%, total phosphorus ≥1.2wt%, and lysine ≥2.5wt%.

[0049] When the mass ratio of the conventional feed to the additive composition is within the above range, it can not only effectively control the feeding cost but also has an excellent effect of treating Chinese sturgeon gill swelling.

[0050] In another aspect, the present invention provides a use of the additive composition as described above in preparing a medicament for treating Chinese sturgeon gill swelling.

[0051] In detail, the additive composition is used in the process of preparing a drug for treating Chinese sturgeon gill swelling.

[0052] The present invention does not limit the specific preparation process of the drug for treating Chinese sturgeon gill swelling, and a common preparation method in the art can be selected.

[0053] In another aspect, the present invention provides a method for feeding Chinese sturgeon, which comprises feeding Chinese sturgeon with the feed as described above.

[0054] Since the Chinese sturgeon is fed with the above feed, the feeding method provided by the present invention can effectively treat the Chinese sturgeon gill swelling disease.

[0055] Furthermore, in a specific embodiment of the present invention, the daily feeding amount during the feeding process is 1.0-1.2% of the body weight, the feeding frequency is 3 times / day, and the feeding time interval is 5-6 hours.

[0056] The daily feeding amount in the present invention refers to the ratio of the mass of feed fed every day to the average mass of Chinese sturgeon.

[0057] The daily feeding amount is 1.0-1.2%. For example, the daily feeding amount includes but is not limited to 1.0%, 1.1%, 1.2% or a range consisting of any two thereof.

[0058] In a specific embodiment, the feeding is carried out at 7:00 every day, and the feeding amount is 0.3-0.4%; the feeding is carried out at 13:00, and the feeding amount is 0.3-0.4%; the feeding is carried out at 19:00, and the feeding amount is 0.3-0.4%.

[0059] It is understandable that the feeding amount and feeding time interval can be adjusted within the above range according to the feeding situation of the Chinese sturgeon.

[0060] When the Chinese sturgeon is fed according to the above feeding method, it can not only maintain the balance of the Chinese sturgeon's intake, allowing the Chinese sturgeon to better digest and absorb the feed and additive composition, but also enable the additive composition to fully exert its effect and improve the cure rate of Chinese sturgeon gill swelling disease.

[0061] Furthermore, in a specific embodiment of the present invention, during the feeding process, the dissolved oxygen content of the aquaculture water is higher than 7.5 mg / L.

[0062] The present invention does not limit the control method of the dissolved oxygen content in the aquaculture water body, and it can be adjusted using a common control method in the art.

[0063] In a specific embodiment, an aerator or liquid oxygen is used to adjust the dissolved oxygen content of the aquaculture water.

[0064] When the dissolved oxygen content in the aquaculture water is higher than 7.5 mg / L, it helps to increase the oxygen supply in the body of the sick Chinese sturgeon, improve the immune system function of the sick Chinese sturgeon, make it more resistant, and increase the cure rate of Chinese sturgeon gill swelling disease.

[0065] In another aspect, the present invention provides a pharmaceutical composition comprising the additive composition as described above.

[0066] Since the pharmaceutical composition provided by the present invention includes the above-mentioned additive composition, it has a significant effect in the process of treating Chinese sturgeon gill swelling disease.

[0067] Furthermore, in a specific embodiment of the present invention, an auxiliary agent is also included, and the auxiliary agent includes 5-30 parts of doxycycline hydrochloride and 5000-10000 parts of salt in parts by weight.

[0068] The doxycycline hydrochloride in the present invention can be purchased in the market, as long as the content of doxycycline hydrochloride is higher than 95%.

[0069] In detail, the weight parts of doxycycline hydrochloride are 5-30 parts. For example, the weight parts of doxycycline hydrochloride include but are not limited to 5 parts, 10 parts, 15 parts, 20 parts, 25 parts, 30 parts or a range consisting of any two of them.

[0070] The doxycycline hydrochloride in the present invention is a long-acting, highly effective, broad-spectrum semi-synthetic tetracycline antibiotic, which, in addition to being effective against Gram-positive and Gram-negative bacteria, can also inhibit the growth of Rickettsia, ameba, etc., and is also sensitive and effective against tetracycline- and oxytetracycline-resistant Staphylococcus aureus.

[0071] The salt in the present invention refers to medical salt, more than 99 wt% of which is sodium chloride, and contains a small amount of calcium ions, magnesium ions and sulfate ions.

[0072] In detail, the weight parts of the salt are 5000-10000. For example, the weight parts of the salt include but are not limited to 5000 parts, 6000 parts, 7000 parts, 8000 parts, 9000 parts, 10000 parts or a range consisting of any two of them.

[0073] After the salt in the present invention is dissolved in water, it can change the osmotic pressure of pathogens or attached organisms, causing imbalance of fluid inside and outside the cells, resulting in death or falling off from the attachment site. It is mainly used to prevent and treat bacterial, fungal or parasitic diseases, and can also prevent and treat brown blood caused by nitrite poisoning.

[0074] The auxiliary agent in the present invention is used for medicinal bathing. During use, the auxiliary agent needs to be dissolved in the aquaculture water body or the treatment water body.

[0075] It is understandable that the appropriate mass-to-volume ratio of the auxiliary agent to the aquaculture water can be selected according to the severity of the gill swelling disease of the Chinese sturgeon and the growth condition of the Chinese sturgeon.

[0076] In one embodiment, the mass volume ratio of the auxiliary agent to the aquaculture water is 5-10 kg / m3 , i.e. 1m 3 Dissolve 5-10kg of auxiliary agent in the aquaculture water.

[0077] When the auxiliary agent is dissolved in the aquaculture water, it needs to be fully stirred and dissolved to make the aquaculture water uniform.

[0078] The auxiliary agent prepared according to specific weight portions of doxycycline hydrochloride and salt can exert a better synergistic effect with the additive composition, treat both internal and external diseases, and has significant therapeutic effects. It can more effectively prevent and control the extremely harmful gill swelling disease of Chinese sturgeon, improve the survival rate of Chinese sturgeon in the breeding process, and promote the protection of Chinese sturgeon.

[0079] The additive composition provided by the present invention is further described below in conjunction with specific examples.

[0080] The Chinese sturgeon in the present invention comes from the Yichang Experimental Station of the Chinese Sturgeon Research Institute.

[0081] Example 1

[0082] The preparation method of the additive composition and auxiliary agent of this embodiment is as follows:

[0083] 1) Prepare the additive composition: Wash and dry the required Chinese herbal medicine, crush it, pass it through a 80-120 mesh sieve, weigh 3kg of roasted Astragalus, 2kg of Codonopsis, 2kg of Atractylodes, 2kg of Angelica, 2kg of Tangerine Peel, 1.5kg of roasted Licorice, 1kg of Cimicifuga, 1kg of Bupleurum, 1kg of Jujube, 1kg of Poria, 1kg of Alisma, 1kg of Coptis, and 1kg of Rhubarb to make Chinese herbal medicine. Then weigh 10.71kg of Chinese herbal medicine, 2.86kg of norfloxacin, and 1.43kg of vitamin added powder, mix them evenly to obtain an additive composition, of which Chinese herbal medicine accounts for 15 parts, norfloxacin accounts for 4 parts, and vitamin added powder accounts for 2 parts;

[0084] Among them, 1kg of vitamin added powder includes 16g of vitamin A, 0.27g of vitamin D3, 10g of vitamin E, 5g of vitamin K3, 2g of vitamin B1, 15g of vitamin B2, 8g of vitamin B6, 0.03g of vitamin B12, 28g of niacin, 25g of calcium pantothenate, 0.5g of folic acid, and 0.16g of biotin.

[0085] 2) Preparation of auxiliary agent: weigh 30 g of doxycycline hydrochloride and 5 kg of salt, mix well to obtain auxiliary agent.

[0086] The above-mentioned additive composition and auxiliary drugs constitute a pharmaceutical composition.

[0087] Example 2

[0088] The preparation method of the additive composition and auxiliary agent of this embodiment is as follows:

[0089] 1) Preparation of additive composition: Wash and dry the required Chinese herbal medicine, crush it, pass through a 80-120 mesh sieve, weigh 3kg of roasted Astragalus, 2kg of Codonopsis, 2kg of Atractylodes, 2kg of Angelica, 2kg of Tangerine Peel, 1.5kg of roasted Licorice, 1kg of Cimicifuga, 1kg of Bupleurum, 1kg of Jujube, 1kg of Poria, 1kg of Alisma, 1kg of Coptis, and 1kg of Rhubarb to make Chinese herbal medicine. Then weigh 9.73kg of Chinese herbal medicine, 4.05kg of norfloxacin, and 1.22kg of vitamin added powder, mix them evenly to obtain an additive composition, wherein Chinese herbal medicine accounts for 12 parts, norfloxacin accounts for 5 parts, and vitamin added powder accounts for 1.5 parts, wherein the vitamin added powder is the same type as in Example 1.

[0090] 2) Preparation of auxiliary agent: weigh 5 g of doxycycline hydrochloride and 10 kg of salt, mix well to obtain auxiliary agent.

[0091] The above-mentioned additive composition and auxiliary drugs constitute a pharmaceutical composition.

[0092] Example 3

[0093] The preparation method of the additive composition and auxiliary agent of this embodiment is as follows:

[0094] 1) Preparation of additive composition: Wash and dry the required Chinese herbal medicine, crush it, pass it through a 80-120 mesh sieve, weigh 3kg of roasted Astragalus, 2kg of Codonopsis, 2kg of Atractylodes, 2kg of Angelica, 2kg of Tangerine Peel, 1.5kg of roasted Licorice, 1kg of Cimicifuga, 1kg of Bupleurum, 1kg of Jujube, 1kg of Poria, 1kg of Alisma, 1kg of Coptis, and 1kg of Rhubarb according to weight to make Chinese herbal medicine. Then weigh 10.00kg of Chinese herbal medicine, 3.00kg of norfloxacin, and 2.00kg of vitamin added powder, mix them evenly to obtain an additive composition, wherein Chinese herbal medicine accounts for 10 parts, norfloxacin accounts for 3 parts, and vitamin added powder accounts for 2 parts, wherein the vitamin added powder is the same type as in Example 1.

[0095] 2) Preparation of auxiliary agent: weigh 10 g of doxycycline hydrochloride and 8 kg of salt, mix well to obtain auxiliary agent.

[0096] The above-mentioned additive composition and auxiliary drugs constitute a pharmaceutical composition.

[0097] Example 4

[0098] The preparation method of the auxiliary agent in this embodiment is the same as that in embodiment 1, and the preparation method of the additive composition is different from that in embodiment 1, which is specifically as follows:

[0099] 1) Preparation of additive composition: Wash and dry the required Chinese herbal medicine, crush it, pass it through a 80-120 mesh sieve, weigh 3kg of roasted Astragalus, 2kg of Codonopsis, 2kg of Atractylodes, 2kg of Angelica, 2kg of Tangerine Peel, 1.5kg of roasted Licorice, 1kg of Cimicifuga, 1kg of Bupleurum, 1kg of Jujube, 1kg of Poria, 1kg of Alisma, 1kg of Coptis, and 1kg of Rhubarb according to weight to make Chinese herbal medicine. Then weigh 10.50kg of Chinese herbal medicine, 3.00kg of norfloxacin, and 1.50kg of vitamin added powder, mix them evenly to obtain an additive composition, wherein Chinese herbal medicine accounts for 14 parts, norfloxacin accounts for 4 parts, and vitamins account for 2 parts, wherein the vitamin added powder is the same type as in Example 1.

[0100] The above-mentioned additive composition and auxiliary drugs constitute a pharmaceutical composition.

[0101] Comparative Example 1

[0102] The preparation method of the auxiliary agent in this comparative example is the same as that in Example 1, except that the drug composition in this comparative example does not include an additive composition.

[0103] Comparative Example 2

[0104] The preparation method of the auxiliary agent in this comparative example is the same as that in Example 1, but the preparation method of the additive composition is different, as follows:

[0105] In step 1), the required Chinese herbal medicine is cleaned and dried, crushed, and passed through a 80-120 mesh sieve. According to weight, 1kg of Radix Astragali, 1kg of Codonopsis, 0.5kg of Atractylodes macrocephala, 0.5kg of Angelica sinensis, 1kg of Tangerine peel, 0.5kg of Radix Glycyrrhizae Preparata, 1kg of Cimicifuga, 0.5kg of Radix Bupleuri, 1kg of Jujube, 1kg of Poria, 0.5kg of Rhizoma Alismatis, 2kg of Coptis chinensis, and 1kg of Rhubarb are weighed to make Chinese herbal medicine. Afterwards, 10.00kg of Chinese herbal medicine is weighed, 3.00kg of norfloxacin is weighed, 2.00kg of vitamin-added powder is weighed, and the additive composition is uniformly obtained, wherein Chinese herbal medicine accounts for 10 parts, norfloxacin accounts for 3 parts, and vitamin-added powder accounts for 2 parts, wherein the vitamin-added powder is the same type as that in Example 1.

[0106] The above-mentioned additive composition and auxiliary drugs constitute a pharmaceutical composition.

[0107] Comparative Example 3

[0108] The preparation method of the auxiliary agent in this comparative example is the same as that in Example 1, but the preparation method of the additive composition is different, as follows:

[0109] In step 1), 3.00 kg of norfloxacin and 1.50 kg of vitamin-added powder are mixed uniformly to obtain an additive composition, wherein the vitamin-added powder is of the same type as that in Example 1.

[0110] The above-mentioned additive composition and auxiliary drugs constitute a pharmaceutical composition.

[0111] Comparative Example 4

[0112] In this comparative example, 3.0 kg of norfloxacin was used as the additive composition, and 30 g of doxycycline hydrochloride was used as the auxiliary agent.

[0113] The above-mentioned additive composition and auxiliary drugs constitute a pharmaceutical composition.

[0114] Comparative Example 5

[0115] In this comparative example, 3.0 kg of vitamin added powder is used as the additive composition, and 30 g of amoxicillin is used as the auxiliary agent, wherein the vitamin added powder is of the same type as that in Example 1.

[0116] The above-mentioned additive composition and auxiliary drugs constitute a pharmaceutical composition.

[0117] Comparative Example 6

[0118] In this comparative example, 2.0 kg of norfloxacin was used as the additive composition, and 5 kg of salt was used as the auxiliary agent.

[0119] The above-mentioned additive composition and auxiliary drugs constitute a pharmaceutical composition.

[0120] Test Case

[0121] The additive composition prepared in the above Examples 1-4 and Comparative Examples 2-6 was mixed with conventional feed at a mass ratio of 1.5:100 to prepare feed.

[0122] Fish diseases occurred in the flow-through breeding workshop of the Yichang Experimental Station of the Chinese Sturgeon Research Institute during 2021-2022. The fish diseases were diagnosed and treated according to the following method. When the drug composition does not contain an additive composition or an auxiliary agent, the agent or the feed including the additive composition is not used for treatment.

[0123] (1) Diagnosis of gill swelling: Chinese sturgeon floats on the upper layer of water and swims slowly and weakly. The breathing rate of the gills is significantly accelerated, the gill filaments are swollen, and the respiratory system is obviously diseased. The mouths of some diseased fish are red, swollen and protruding, and there are varying degrees of congestion on the body surface. Dissection shows congestion of the liver, the outer wall of the swim bladder, and the inner and outer walls of the intestines.

[0124] (2) Use auxiliary drugs for medicated bath treatment: All fish in the diseased fish pond are transferred to the treatment pond for treatment. The transfer of diseased fish needs to be gentle and carried out with water to minimize the stress response. The treatment pond is equipped with oxygenation facilities, using an aerator or liquid oxygen to increase oxygen. During the treatment process, the dissolved oxygen content in the pond is maintained at >7.5mg / L. According to 5-10kg / m 3Dissolve the auxiliary agent in the treatment pool water in a certain proportion, soak the sick fish in the medicine, and put them back into the disinfected original breeding pool after soaking for 2 hours. Follow this method to soak the fish once a day, and one course of treatment is 5-7 days of continuous use.

[0125] (3) Disinfection of the original breeding pond: drain the water from the original breeding pond, clean the breeding pond, inject new water into the pond after cleaning, use 30-50 ppm of povidone for disinfection, drain the waste water after disinfection, rinse the breeding pond with new water three times, and inject new water.

[0126] (4) Feeding. The sick fish treated in step (2) are returned to the disinfected culture pond for feeding, and are fed with feed containing the additive composition or conventional feed, with a daily feeding amount of 1.2%, 3 times a day, 0.4% each time, at 7:00, 13:00, and 19:00, and a feeding cycle of 7-14 days is one course of treatment.

[0127] After 1-2 courses of treatment according to the above method, the condition of the diseased fish was observed. The specific results are shown in Table 1.

[0128] Table 1

[0129]

[0130]

[0131] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. An additive composition, characterized in that The additive composition consists of 10-15 parts of Chinese herbal medicine, 3-5 parts of norfloxacin, and 1.5-3 parts of vitamin additive powder by weight; the formula of the Chinese herbal medicine is as follows: roasted astragalus, codonopsis pilosula, atractylodes macrocephala, angelica sinensis, tangerine peel, roasted licorice, cimicifuga, bupleurum, jujube, poria, oriental water plantain, coptis chinensis, and rhubarb, with a mass ratio of 3:2:2:2:2:1.5:1:1:1:1:1:1:1:

1.

2. The additive composition according to claim 1, characterized in that The weight portion of the Chinese herbal medicine is 15 parts, the weight portion of norfloxacin is 4 parts, and the weight portion of the vitamin added powder is 2 parts.

3. Use of the additive composition according to claim 1 or 2 in preparing a medicament for treating Chinese sturgeon gill swelling.

4. A pharmaceutical composition, characterized in that The additive composition comprising claim 1 or 2.

5. The pharmaceutical composition according to claim 4, characterized in that The invention also comprises auxiliary agents, which include 5-30 parts of doxycycline hydrochloride and 5000-10000 parts of salt in parts by weight.

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