A preparation method of Polygonum multiflorum Qinghu granules for treating blue ear disease in pigs

Abstract

The invention provides a preparation method of Bistort and Green Tiger Granules for blue ear disease in pigs, and the preparation method comprises the following steps: S1, taking dried Bistort, Terminalia chebula, Polygonum cuspidatum, Folium Isatidis, Folium Chrysanthemi, and Folium Eucommia for crushing to obtain a mixed crushed material; S2, placing the mixed crushed material in an ethanol / water mixed solution, ultrasonic extraction, ultrahigh pressure extraction; filtering to obtain a filtrate 1 and a filter residue 1; the filtrate 1 is decompressed and concentrated to obtain a concentrated solution 1; S3, placing the filter residue 1 in water, ultrahigh pressure extraction; filtering to obtain a filtrate 2 and a filter residue 2; the filtrate 2 is decompressed and concentrated to obtain a concentrated solution 2; S4, adding ethanol to the filter residue 2 and then adding ethyl acetate, ultrahigh pressure extraction; filtering to obtain a filtrate 3; the filtrate 3 is decompressed and concentrated, dried to obtain an extract; S5, mixing the concentrate 1 and the concentrate 2, adding the extract and auxiliary materials, mixing evenly, and granulating. The Bistort and Green Tiger Granules prepared by the invention can effectively treat blue ear disease, with a high overall cure rate and good safety.

Description

Technical Field

[0001] The invention relates to the technical field of traditional Chinese medicines, and in particular to a method for preparing Polysaccharide Recutita chinensis Qinghu granules for treating blue ear disease in pigs. Background Art

[0002] Blue ear disease is an infectious disease caused by porcine reproductive and respiratory syndrome virus (PRRSV). Blue ear disease often occurs in summer and autumn. The main clinical symptoms include reproductive disorders, breathing difficulties, blue and purple ears, etc., and may also be complicated by other infectious diseases. The disease is not only transmitted between fattening pigs, but also between breeding pigs and piglets, and can even infect suckling piglets. The disease can cause high mortality in pigs and can easily cause mixed infections, leading to the occurrence of many other diseases.

[0003] With the development of pig farming, the scale and number of pigs raised have continued to expand and increase. In some large-scale farms with a high breeding density, once blue ear disease occurs, the disease will spread rapidly in the farms, threatening the orderly progress of breeding work. Faced with the high incidence of blue ear disease, farmers need to strengthen prevention and control to reduce the adverse effects of the disease.

[0004] As a veterinary medicine, the main ingredients of the Polygonum multiflorum Qinghu Granules include Chinese medicinal materials such as Polygonum multiflorum, Folium Isatidis, Polygonum cuspidatum, and Terminalia chebula. These ingredients have the effects of clearing away heat and detoxifying, resolving phlegm, relieving cough and relieving asthma. During the treatment of porcine blue ear disease, if pigs show symptoms related to lung heat, cough and asthma, such as coughing and asthma, Polygonum multiflorum Qinghu Granules can be used for auxiliary treatment. However, the current Polygonum multiflorum Qinghu Granules have limited therapeutic effects on porcine blue ear disease. Therefore, in order to further improve the therapeutic effect of Polygonum multiflorum Qinghu Granules on porcine blue ear disease, it is necessary to modify its formula and preparation method to obtain Polygonum multiflorum Qinghu Granules with better efficacy. Summary of the invention

[0005] The purpose of the present invention is to provide a preparation method of Polysaccharide Recutita Green Tiger Granules for treating blue ear disease in pigs. The prepared Polysaccharide Recutita Green Tiger Granules can effectively treat blue ear disease, have a high overall cure rate, and are safe. The production cycle of the Polysaccharide Recutita Green Tiger Granules is short, and the method is relatively simple to operate.

[0006] To achieve the above objectives, the present invention is implemented through the following technical solutions:

[0007] A method for preparing Bistorta Qinghu granules for treating blue ear disease in pigs, comprising the following steps:

[0008] S1, taking dried scutellaria baicalensis, terminalia chebula, knotweed, isatis indigo plant, chrysanthemum, and eucommia leaf and grinding them to obtain a mixed grind;

[0009] S2, placing the mixed crushed material in an ethanol / water mixture, first performing ultrasonic extraction, and then performing ultrahigh pressure extraction; then filtering to obtain a filtrate 1 and a filter residue 1; and concentrating the filtrate 1 under reduced pressure to obtain a concentrate 1;

[0010] S3, placing the filter residue 1 in water, and then performing ultra-high pressure extraction; filtering to obtain filtrate 2 and filter residue 2; and concentrating the filtrate 2 under reduced pressure to obtain concentrated solution 2;

[0011] S4, adding ethanol and then ethyl acetate to the filter residue 2, and then performing ultra-high pressure extraction; filtering to obtain filtrate 3 and filter residue 3; concentrating and drying the filtrate 3 under reduced pressure to obtain an extract;

[0012] S5. After the concentrated solution 1 and the concentrated solution 2 are mixed, the extract and auxiliary materials are added, the mixture is evenly mixed, and the Polygonum multiflorum Qinghu Granules are obtained by granulation.

[0013] Preferably, in step S1, the mass ratio of the Polygonum multiflorum, Folium Isatidis, Polygonum cuspidatum, Fructus Terminaliae Terminaliae, Folium Eucommiae is 300-450:250-400:80-130:150-250:150-300:200-500.

[0014] Further preferably, the mass ratio of the Polygonum multiflorum, Folium Isatidis, Polygonum cuspidatum, Fructus Terminaliae Terminaliae, Fructus Chrysanthemi Indigoferae, and Folium Eucommiae is 400:300:100:200:200:300.

[0015] Preferably, in step S1, the particle size of the mixed crushed material is less than 50 mesh.

[0016] Preferably, in step S2, the power of the ultrasonic extraction is 300-450 W, and the time is 35-45 min; the pressure of the ultrahigh pressure extraction is 300-450 MPa, and the time is 5-7 min.

[0017] Preferably, in step S3, the pressure of the ultra-high pressure extraction is 400-500 MPa, and the time is 2-3 min; in step S4, the pressure of the ultra-high pressure extraction is 300-400 MPa, and the time is 2-3 min.

[0018] Preferably, in step S2, the mass volume ratio of the mixed crushed material to the ethanol / water mixed liquid is 1 kg: 6-8 L, the ethanol / water mixed liquid is prepared by mixing ethanol and water, and the volume fraction of ethanol in the ethanol / water mixed liquid is 60-70%;

[0019] In step S3, the mass volume ratio of the filter residue 1 to water is 1kg:3.5-4.5L.

[0020] In step S4, the mass volume ratio of the filter residue 2 to ethanol and ethyl acetate is 1kg:1-1.5L:2.5-3L.

[0021] Preferably, in step S2, the temperature during the reduced pressure concentration is 60~65°C, the vacuum degree is -0.08~-0.06Mpa, and the relative density of the concentrated solution 1 is 1.05~1.10; in step S3, the temperature during the reduced pressure concentration is 70~75°C, the vacuum degree is -0.08~-0.06Mpa, and the relative density of the concentrated solution 2 is 1.05~1.10; in step S4, the temperature during the reduced pressure concentration is 55~60°C, and the vacuum degree is -0.08~-0.06Mpa.

[0022] Preferably, the auxiliary materials include β-cyclodextrin, microcrystalline cellulose and sucrose, and the mass ratio of the β-cyclodextrin, microcrystalline cellulose and sucrose is 1:0.5~1:2~2.5.

[0023] Preferably, step S5 specifically includes the following steps: mixing concentrated solution 1 and concentrated solution 2 to obtain a mixed concentrated solution; adding the extract and auxiliary materials accounting for 15-19% of the mass of the mixed concentrated solution to the mixed concentrated solution, mixing evenly, and spray granulating to obtain the Polygonum multiflorum Qinghu granules.

[0024] The inlet air temperature of the spray granulation is 120-125°C, and the outlet air temperature is 75-80°C.

[0025] The beneficial effects of the present invention are:

[0026] 1. The present invention adds chrysanthemum and eucommia leaf to bistort, terminalia chebula, knotweed and isatis leaf. The chrysanthemum polyphenols contained in bistort have excellent antiviral, antibacterial, anti-inflammatory and immune function regulating effects, while the flavonoids, polysaccharides, cyclopentane, lignans, chlorogenic acid and the like in eucommia leaf also have excellent antiviral and antibacterial effects. Through the mutual cooperation of the above-mentioned Chinese medicines, the bistort green tiger granules prepared by the formula can effectively enhance the pig's own immunity, can well inhibit the proliferation of porcine blue ear disease virus in the pig body, and have a good therapeutic effect on pigs infected with blue ear disease.

[0027] 2. When preparing the scutellaria baicalensis Qinghu granules, the present invention first performs an ultrasonic extraction, which can cause the plant cell tissue to break or deform. When performing the subsequent ultra-high pressure extraction, combined with the effect of ultra-high pressure, the extraction solvent can easily penetrate into the cells, and the internal structure of the cells is further changed to cause cell division, and the intracellular substances are released into the extraction solvent, so that the extraction rate is significantly increased. And in the subsequent high-pressure extraction, the present invention first uses an ethanol / water mixture for ultra-high pressure extraction, then uses water for ultra-high pressure extraction, and finally uses an ethanol / ethyl acetate mixture for ultra-high pressure extraction. The ultra-high pressure extraction is performed after the above-mentioned solvents are matched, which not only improves the extraction rate, but also allows the obtained effective ingredients to contain more effective ingredients. Compared with only using an ethanol / water mixture and water for ultra-high pressure extraction, the prepared scutellaria baicalensis Qinghu granules can have a better therapeutic effect on blue ear disease.

[0028] 3. The overall extraction time of the present invention is short, the production cycle is short, the method operation is relatively simple, and it is easy to realize automation and large-scale production.

[0029] 4. When preparing the Polygonum multiflorum Qinghu granules of the present invention, an appropriate amount of auxiliary material carrier consisting of β-cyclodextrin, microcrystalline cellulose and sucrose is added to make the prepared granules have higher fluidity.

[0030] 5. The Quan Shen Qinghu granules prepared by the present invention can effectively treat blue ear disease, with a high overall cure rate and good safety. DETAILED DESCRIPTION

[0031] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be clearly and completely described below in combination with the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0032] Embodiment 1:

[0033] A method for preparing Bistorta Qinghu granules for treating blue ear disease in pigs, comprising the following steps:

[0034] S1. Take dried scutellaria baicalensis, chebula chebula, giant knotweed, isatis indigo leaf, chrysanthemum officinale, and eucommia leaf and grind them to obtain a mixed grind with a particle size of less than 50 mesh; the mass ratio of the scutellaria baicalensis, isatis indigo leaf, giant knotweed, chebula chebula, chrysanthemum officinale, and eucommia leaf is 400:300:100:200:200:300.

[0035] S2, placing the mixed crushed material in an ethanol / water mixture, wherein the mass volume ratio of the mixed crushed material to the ethanol / water mixture is 1 kg:8 L, the ethanol / water mixture is prepared by mixing ethanol and water, and the volume fraction of ethanol in the ethanol / water mixture is 65%;

[0036] First, ultrasonic extraction is performed, the power of the ultrasonic extraction is 450W, and the time is 35 minutes; then ultra-high pressure extraction is performed, the pressure of the ultra-high pressure extraction is 450MPa, and the time is 6 minutes; then filtration is performed to obtain filtrate 1 and filter residue 1; the filtrate 1 is concentrated under reduced pressure, the temperature during the reduced pressure concentration is 65°C, the vacuum degree is -0.08Mpa, and a concentrated solution 1 with a relative density of 1.05 is obtained.

[0037] S3, placing the filter residue 1 in water, the mass volume ratio of the filter residue 1 to water is 1kg:4.5L;

[0038] Then, ultra-high pressure extraction was performed, the pressure of which was 400 MPa, and the time was 3 min; the extraction was then filtered to obtain filtrate 2 and residue 2; the filtrate 2 was concentrated under reduced pressure, the temperature during the concentration under reduced pressure was 75° C., the vacuum degree was -0.08 MPa, and a concentrated solution 2 with a relative density of 1.08 was obtained.

[0039] S4, adding ethanol to the filter residue 2 and then adding ethyl acetate, the mass volume ratio of the filter residue 2 to ethanol and ethyl acetate is 1kg:1.5L:2.5L;

[0040] Then, ultra-high pressure extraction is carried out, the pressure of ultra-high pressure extraction is 350MPa, and the time is 2.5min; then filtration is carried out to obtain filtrate three and filter residue three; filtrate three is concentrated and dried under reduced pressure, the temperature during the concentration under reduced pressure is 60°C, and the vacuum degree is -0.07Mpa, to obtain an extract.

[0041] S5, after the concentrate one and the concentrate two are mixed, a mixed concentrate is obtained; the extract and 18% of the mass of the mixed concentrate of the auxiliary materials are added to the mixed concentrate, mixed evenly, and granulated by spraying to obtain the Bistort Green Tiger Granules;

[0042] The auxiliary materials are composed of β-cyclodextrin, microcrystalline cellulose and sucrose in a mass ratio of 1:0.8:2; the inlet air temperature of the spray granulation is 125°C and the outlet air temperature is 75°C.

[0043] Embodiment 2:

[0044] A method for preparing Bistorta Qinghu granules for treating blue ear disease in pigs, comprising the following steps:

[0045] S1. Take the dried scutellaria baicalensis, chebula chebula, giant knotweed, isatis indigotica, chrysanthemum indica, and eucommia leaf and grind them to obtain a mixed grind with a particle size of less than 50 mesh; the mass ratio of the scutellaria baicalensis, isatis indigotica, giant knotweed, chebula chebula, chrysanthemum indica, and eucommia leaf is 450:300:90:150:250:400.

[0046] S2, placing the mixed crushed material in an ethanol / water mixture, wherein the mass volume ratio of the mixed crushed material to the ethanol / water mixture is 1 kg:7 L, the ethanol / water mixture is prepared by mixing ethanol and water, and the volume fraction of ethanol in the ethanol / water mixture is 70%;

[0047] First, ultrasonic extraction is performed, the power of the ultrasonic extraction is 300W, and the time is 45 minutes; then ultra-high pressure extraction is performed, the pressure of the ultra-high pressure extraction is 350MPa, and the time is 6 minutes; then filtration is performed to obtain filtrate 1 and filter residue 1; the filtrate 1 is concentrated under reduced pressure, the temperature during the reduced pressure concentration is 62°C, the vacuum degree is -0.08Mpa, and a concentrated solution 1 with a relative density of 1.06 is obtained.

[0048] S3, placing the filter residue 1 in water, the mass volume ratio of the filter residue 1 to the water is 1kg:4L;

[0049] Then, ultra-high pressure extraction was performed, the pressure of which was 450 MPa, and the time was 3 min; the extraction was then filtered to obtain filtrate 2 and residue 2; filtrate 2 was concentrated under reduced pressure, the temperature during the concentration under reduced pressure was 75° C., the vacuum degree was -0.06 MPa, and concentrated solution 2 with a relative density of 1.09 was obtained.

[0050] S4, adding ethanol to the filter residue 2 and then adding ethyl acetate, the mass volume ratio of the filter residue 2 to ethanol and ethyl acetate is 1kg:1.2L:2.8L;

[0051] Then, ultra-high pressure extraction is carried out, the pressure of ultra-high pressure extraction is 350MPa, and the time is 3min; then filtration is carried out to obtain filtrate three and filter residue three; filtrate three is concentrated and dried under reduced pressure, the temperature during the concentration under reduced pressure is 60°C, and the vacuum degree is -0.06Mpa, to obtain an extract.

[0052] S5, after the concentrate one and the concentrate two are mixed, a mixed concentrate is obtained; the extract and 15% of the mass of the auxiliary materials of the mixed concentrate are added to the mixed concentrate, mixed evenly, and granulated by spraying to obtain the Bistort Green Tiger Granules;

[0053] The auxiliary materials are composed of β-cyclodextrin, microcrystalline cellulose and sucrose in a mass ratio of 1:0.5:2; the inlet air temperature of the spray granulation is 125°C and the outlet air temperature is 80°C.

[0054] Embodiment 3:

[0055] A method for preparing Bistorta Qinghu granules for treating blue ear disease in pigs, comprising the following steps:

[0056] S1. Take the dried scutellaria baicalensis, chebula chebula, giant knotweed, isatis indigotica, chrysanthemum indica, and eucommia leaf and grind them to obtain a mixed grind with a particle size of less than 50 mesh; the mass ratio of the scutellaria baicalensis, isatis indigotica, giant knotweed, chebula chebula, chrysanthemum indica, and eucommia leaf is 350:350:120:220:250:350.

[0057] S2, placing the mixed crushed material in an ethanol / water mixture, wherein the mass volume ratio of the mixed crushed material to the ethanol / water mixture is 1 kg:8 L, the ethanol / water mixture is prepared by mixing ethanol and water, and the volume fraction of ethanol in the ethanol / water mixture is 70%;

[0058] First, ultrasonic extraction is performed, the power of the ultrasonic extraction is 450W, and the time is 40 minutes; then ultra-high pressure extraction is performed, the pressure of the ultra-high pressure extraction is 400MPa, and the time is 6 minutes; then filtration is performed to obtain filtrate 1 and filter residue 1; the filtrate 1 is concentrated under reduced pressure, the temperature during the reduced pressure concentration is 65°C, the vacuum degree is -0.06Mpa, and a concentrated solution 1 with a relative density of 1.08 is obtained.

[0059] S3, placing the filter residue 1 in water, the mass volume ratio of the filter residue 1 to water is 1kg:4.5L;

[0060] Then, ultra-high pressure extraction was performed, the pressure of which was 450 MPa, and the time was 3 min; the extraction was then filtered to obtain filtrate 2 and residue 2; filtrate 2 was concentrated under reduced pressure, the temperature during the concentration under reduced pressure was 75° C., the vacuum degree was -0.07 MPa, and concentrated solution 2 with a relative density of 1.07 was obtained.

[0061] S4, adding ethanol to the filter residue 2 and then adding ethyl acetate, the mass volume ratio of the filter residue 2 to ethanol and ethyl acetate is 1kg:1.5L:2.5L;

[0062] Then, ultra-high pressure extraction is carried out, the pressure of ultra-high pressure extraction is 350MPa, and the time is 3min; then filtration is carried out to obtain filtrate three and filter residue three; filtrate three is concentrated and dried under reduced pressure, the temperature during the concentration under reduced pressure is 60°C, and the vacuum degree is -0.08Mpa, to obtain an extract.

[0063] S5, after the concentrate 1 and the concentrate 2 are mixed, a mixed concentrate is obtained; the extract and 17% of the mass of the mixed concentrate of the auxiliary materials are added to the mixed concentrate, mixed evenly, and granulated by spraying to obtain the scutellaria baicalensis green tiger granules;

[0064] The auxiliary materials are composed of β-cyclodextrin, microcrystalline cellulose and sucrose in a mass ratio of 1:0.8:2; the inlet air temperature of the spray granulation is 122°C and the outlet air temperature is 75°C.

[0065] Embodiment 4:

[0066] A method for preparing Bistorta Qinghu granules for treating blue ear disease in pigs, comprising the following steps:

[0067] S1. Take the dried scutellaria baicalensis, chebula chebula, giant knotweed, isatis indigotica, chrysanthemum officinale, and eucommia leaf and grind them to obtain a mixed grind with a particle size of less than 50 mesh; the mass ratio of the scutellaria baicalensis, isatis indigotica, giant knotweed, chebula chebula, chrysanthemum officinale, and eucommia leaf is 450:250:100:220:150:400.

[0068] S2, placing the mixed crushed material in an ethanol / water mixture, wherein the mass volume ratio of the mixed crushed material to the ethanol / water mixture is 1 kg:8 L, the ethanol / water mixture is prepared by mixing ethanol and water, and the volume fraction of ethanol in the ethanol / water mixture is 65%;

[0069] First, ultrasonic extraction is performed, the power of the ultrasonic extraction is 450W, and the time is 35 minutes; then ultra-high pressure extraction is performed, the pressure of the ultra-high pressure extraction is 300MPa, and the time is 7 minutes; then filtration is performed to obtain filtrate 1 and filter residue 1; the filtrate 1 is concentrated under reduced pressure, the temperature during the reduced pressure concentration is 65°C, the vacuum degree is -0.07Mpa, and a concentrated solution 1 with a relative density of 1.08 is obtained.

[0070] S3, placing the filter residue 1 in water, the mass volume ratio of the filter residue 1 to water is 1kg:4.5L;

[0071] Then, ultra-high pressure extraction was performed, the pressure of which was 400 MPa, and the time was 3 min; then, filtration was performed to obtain filtrate 2 and filter residue 2; filtrate 2 was concentrated under reduced pressure, the temperature during the concentration under reduced pressure was 70° C., the vacuum degree was -0.08 MPa, and concentrated solution 2 with a relative density of 1.05 was obtained.

[0072] S4, adding ethanol to the filter residue 2 and then adding ethyl acetate, the mass volume ratio of the filter residue 2 to ethanol and ethyl acetate is 1kg:1.5L:2.5L;

[0073] Then, ultra-high pressure extraction is carried out, the pressure of ultra-high pressure extraction is 400MPa, and the time is 3min; then filtration is carried out to obtain filtrate three and filter residue three; filtrate three is concentrated and dried under reduced pressure, the temperature during the concentration under reduced pressure is 55°C, and the vacuum degree is -0.08Mpa, to obtain an extract.

[0074] S5, after the concentrate 1 and the concentrate 2 are mixed, a mixed concentrate is obtained; the extract and 19% of the auxiliary materials by weight of the mixed concentrate are added to the mixed concentrate, the mixture is evenly mixed, and the mixture is granulated by spraying to obtain the Bistort Green Tiger Granules;

[0075] The auxiliary materials are composed of β-cyclodextrin, microcrystalline cellulose and sucrose in a mass ratio of 1:0.5:2; the inlet air temperature of the spray granulation is 125°C and the outlet air temperature is 80°C.

[0076] Embodiment 5:

[0077] A method for preparing Bistorta Qinghu granules for treating blue ear disease in pigs, comprising the following steps:

[0078] S1. Take the dried scutellaria baicalensis, chebula chebula, giant knotweed, isatis indigotica, chrysanthemum officinale, and eucommia leaf and grind them to obtain a mixed grind with a particle size of less than 50 mesh; the mass ratio of the scutellaria baicalensis, isatis indigotica, giant knotweed, chebula chebula, chrysanthemum officinale, and eucommia leaf is 300:300:80:150:200:500.

[0079] S2, placing the mixed crushed material in an ethanol / water mixture, wherein the mass volume ratio of the mixed crushed material to the ethanol / water mixture is 1 kg:8 L, the ethanol / water mixture is prepared by mixing ethanol and water, and the volume fraction of ethanol in the ethanol / water mixture is 60%;

[0080] First, ultrasonic extraction is performed, the power of the ultrasonic extraction is 300W, and the time is 45 minutes; then ultra-high pressure extraction is performed, the pressure of the ultra-high pressure extraction is 450MPa, and the time is 5 minutes; then filtration is performed to obtain a filtrate 1 and a filter residue 1; the filtrate 1 is concentrated under reduced pressure, the temperature during the reduced pressure concentration is 60°C, the vacuum degree is -0.08Mpa, and a concentrated solution 1 with a relative density of 1.05 is obtained.

[0081] S3, placing the filter residue 1 in water, the mass volume ratio of the filter residue 1 to water is 1kg:3.5L;

[0082] Then, ultra-high pressure extraction was performed, the pressure of which was 500 MPa, and the time was 2 min; the extraction was then filtered to obtain filtrate 2 and residue 2; the filtrate 2 was concentrated under reduced pressure, the temperature during the concentration under reduced pressure was 75° C., the vacuum degree was -0.06 MPa, and a concentrated solution 2 with a relative density of 1.05 was obtained.

[0083] S4, adding ethanol to the filter residue 2 and then adding ethyl acetate, the mass volume ratio of the filter residue 2 to ethanol and ethyl acetate is 1kg:1L:3L;

[0084] Then, ultra-high pressure extraction is carried out, the pressure of ultra-high pressure extraction is 300MPa, and the time is 2min; then filtration is carried out to obtain filtrate three and filter residue three; filtrate three is concentrated and dried under reduced pressure, the temperature during the concentration under reduced pressure is 60°C, and the vacuum degree is -0.06Mpa, to obtain an extract.

[0085] S5, after the concentrate one and the concentrate two are mixed, a mixed concentrate is obtained; the extract and 15% of the mass of the auxiliary materials of the mixed concentrate are added to the mixed concentrate, mixed evenly, and granulated by spraying to obtain the Bistort Green Tiger Granules;

[0086] The auxiliary materials are composed of β-cyclodextrin, microcrystalline cellulose and sucrose in a mass ratio of 1:0.5:2; the inlet air temperature of the spray granulation is 120°C and the outlet air temperature is 75°C.

[0087] Embodiment 6:

[0088] A method for preparing Bistorta Qinghu granules for treating blue ear disease in pigs, comprising the following steps:

[0089] S1. Take the dried scutellaria baicalensis, chebula chebula, giant knotweed, isatis indigotica, chrysanthemum officinale, and eucommia leaf and grind them to obtain a mixed grind with a particle size of less than 50 mesh; the mass ratio of the scutellaria baicalensis, isatis indigotica, giant knotweed, chebula chebula, chrysanthemum officinale, and eucommia leaf is 350:400:130:250:300:200.

[0090] S2, placing the mixed crushed material in an ethanol / water mixture, wherein the mass volume ratio of the mixed crushed material to the ethanol / water mixture is 1 kg:6 L, the ethanol / water mixture is prepared by mixing ethanol and water, and the volume fraction of ethanol in the ethanol / water mixture is 70%;

[0091] First, ultrasonic extraction is performed, the power of the ultrasonic extraction is 450W, and the time is 35 minutes; then ultra-high pressure extraction is performed, the pressure of the ultra-high pressure extraction is 350MPa, and the time is 7 minutes; then filtration is performed to obtain filtrate 1 and filter residue 1; the filtrate 1 is concentrated under reduced pressure, the temperature during the reduced pressure concentration is 65°C, the vacuum degree is -0.06Mpa, and a concentrated solution 1 with a relative density of 1.10 is obtained.

[0092] S3, placing the filter residue 1 in water, the mass volume ratio of the filter residue 1 to the water is 1kg:4L;

[0093] Then, ultra-high pressure extraction was performed, the pressure of which was 500 MPa, and the time was 2 min; the extraction was filtered to obtain filtrate 2 and residue 2; filtrate 2 was concentrated under reduced pressure, the temperature during the concentration under reduced pressure was 70° C., the vacuum degree was -0.08 MPa, and concentrated solution 2 with a relative density of 1.10 was obtained.

[0094] S4, adding ethanol to the filter residue 2 and then adding ethyl acetate, the mass volume ratio of the filter residue 2 to ethanol and ethyl acetate is 1kg:1.5L:2.5L;

[0095] Then, ultra-high pressure extraction is carried out, the pressure of ultra-high pressure extraction is 350MPa, and the time is 3min; then filtration is carried out to obtain filtrate three and filter residue three; filtrate three is concentrated and dried under reduced pressure, the temperature during the concentration under reduced pressure is 60°C, and the vacuum degree is -0.07Mpa, to obtain an extract.

[0096] S5, after the concentrate one and the concentrate two are mixed, a mixed concentrate is obtained; the extract and 18% of the mass of the mixed concentrate of the auxiliary materials are added to the mixed concentrate, mixed evenly, and granulated by spraying to obtain the Bistort Green Tiger Granules;

[0097] The auxiliary materials are composed of β-cyclodextrin, microcrystalline cellulose and sucrose in a mass ratio of 1:1:2.5. The inlet air temperature of the spray granulation is 125°C and the outlet air temperature is 75°C.

[0098] Comparative Example 1:

[0099] A method for preparing Bistorta Qinghu granules for treating blue ear disease in pigs, comprising the following steps:

[0100] S1. Take the dried scutellaria baicalensis, terminalia chebula, knotweed and isatis indigotica and grind them to obtain a mixed grind with a particle size of less than 50 mesh; the mass ratio of the scutellaria baicalensis, isatis indigotica, knotweed and terminalia chebula is 400:300:100:200.

[0101] S2, placing the mixed crushed material in an ethanol / water mixture, wherein the mass volume ratio of the mixed crushed material to the ethanol / water mixture is 1 kg:8 L, the ethanol / water mixture is prepared by mixing ethanol and water, and the volume fraction of ethanol in the ethanol / water mixture is 65%;

[0102] First, ultrasonic extraction is performed, the power of the ultrasonic extraction is 450W, and the time is 35 minutes; then ultra-high pressure extraction is performed, the pressure of the ultra-high pressure extraction is 450MPa, and the time is 6 minutes; then filtration is performed to obtain filtrate 1 and filter residue 1; the filtrate 1 is concentrated under reduced pressure, the temperature during the reduced pressure concentration is 65°C, the vacuum degree is -0.08Mpa, and a concentrated solution 1 with a relative density of 1.05 is obtained.

[0103] S3, placing the filter residue 1 in water, the mass volume ratio of the filter residue 1 to water is 1kg:4.5L;

[0104] Then, ultrahigh pressure extraction was performed, the pressure of which was 400 MPa, and the time was 3 min; then, filtration was performed to obtain filtrate 2 and filter residue 2; the filtrate 2 was concentrated under reduced pressure, the temperature during the concentration under reduced pressure was 75° C., the vacuum degree was -0.08 MPa, and a concentrated solution 2 with a relative density of 1.08 was obtained.

[0105] S4, after the concentrate 1 and the concentrate 2 are mixed, a mixed concentrate is obtained; 18% of the mass of the mixed concentrate is added to the mixed concentrate, the auxiliary materials are mixed evenly, and the mixture is granulated by spraying to obtain the Bistort Green Tiger Granules;

[0106] The auxiliary materials are composed of β-cyclodextrin, microcrystalline cellulose and sucrose in a mass ratio of 1:0.8:2. The inlet air temperature of the spray granulation is 125°C and the outlet air temperature is 75°C.

[0107] 1. Acute toxicity test

[0108] The acute toxicity test was conducted on the bistort green tiger granules prepared in Examples 1-6 of the present invention and Comparative Example 1. The test used mice as experimental animals to observe the oral toxicity of the bistort green tiger granules of the present invention to the test animals. During the test, the bistort green tiger granules of the present invention were dissolved in distilled water and then gavaged to mice at 5g / kg body weight. The test results showed that the bistort green tiger granules of the present invention did not cause the death of the test animals, that is, the LD50 of the bistort green tiger granules of the present invention in the oral acute toxicity test on mice was 1.34. 50 Greater than 5g / kg body weight, proving that it is actually non-toxic and has a high degree of safety.

[0109] 2. Evaluation test of clinical treatment effect of porcine reproductive and respiratory syndrome

[0110] A pig farm in Zhejiang Province has 215 nursery pigs (about 50 days old). The main symptoms are heaping, coughing, and fever (40.0-41℃) in the early stage of the disease; tear marks in the corners of the eyes, rough fur; difficulty breathing, abdominal breathing, and bleeding spots on the skin under the abdomen in the middle and late stages; and cyanosis of the edges of the ears.

[0111] Anatomical lesions: Flesh changes occur in the apical lobe, cardiac lobe and diaphragmatic lobe near the cardiac lobe, the apical lobe of the lung becomes longer, diffuse interstitial pneumonia occurs in the large lobes of the lung, and turns grayish white after a while, the pulmonary interstitium widens, there is pericardial effusion, pleural effusion, fibrous exudate on the lungs and liver, and fibrous exudate in the peritoneal effusion.

[0112] Pathogen identification: Lung, lymph node and other pathological samples were collected for testing, and the main pathogens were determined to be: highly pathogenic blue ear disease virus, Mycoplasma pneumoniae, and Haemophilus parasuis.

[0113] The positive rate of blue ear disease in nursery pigs was 85% and the dispersion was 52.

[0114] Medication plan for nursery pigs:

[0115] Mix and feed for 7 consecutive days.

[0116] Experimental group: 2 kg / ton of the Quanshen Qinghu granules in Example 1, 2 kg / ton of 10% amoxicillin, and 2 kg / ton of 10% enrofloxacin.

[0117] Control group 1: 10% doxycycline 1kg / ton, 5% tyvalosin 2kg / ton.

[0118] Control group 2: 2kg / ton of Quanshen Qinghu granules, 2kg / ton of 10% amoxicillin, and 2kg / ton of 10% enrofloxacin in comparative example 1.

[0119] The 215 nursery pigs were randomly divided into the experimental group, the control group 1 and the control group 2. The number of nursery pigs in the experimental group, the control group 1 and the control group 2 were 70, 75 and 70 respectively, and they were divided into 7-8 pens for separate feeding. During the feeding process, the pig house was kept clean, hygienic, well ventilated and at a suitable temperature.

[0120] Validity results determination:

[0121] After 7 days of medication, observe the pigs in each group of nursery pigs. The medication is considered effective when the temperature reaches normal, no respiratory symptoms appear, the fur spirit begins to change, and the death rate decreases to no death. The summary table of the average daily temperature of nursery pigs, the summary table of the average daily feed intake of nursery pigs, the number of nursery pigs before and after medication, the summary table of the effectiveness rate, and the medication are shown in Tables 1-3 respectively.

[0122] Table 1 Summary of average daily temperature of nursery pigs

[0123] Day 1 Day 2 Day 3 Day 4 Day 5 Day 6 Day 7 Experimental Group 40.7℃ 39.6℃ 39.4℃ 39.4℃ 39.3℃ 39.3℃ 39.2℃ Control group 1 40.6℃ 40.4℃ 40.3℃ 40.1℃ 40.0℃ 39.7℃ 39.8℃ Control group 2 40.6℃ 40.0℃ 39.9℃ 39.7℃ 39.7℃ 39.5℃ 39.5℃

[0124] Table 2 Summary of average daily feed intake of nursery pigs

[0125] Day 1 Day 2 Day 3 Day 4 Day 5 Day 6 Day 7 Experimental Group 325g 440g 495g 525g 585g 620g 660g Control group 1 320g 310g 335g 395g 410g 405g 420g Control group 2 320g 410g 465g 490g 560g 595g 625g

[0126] Table 3 Summary of the number and effectiveness of nursery pigs before and after medication

[0127] Group Quantity before test Quantity after test Deaths mortality rate Efficiency Experimental Group 70 heads 68 heads 2 heads 2.9% 97.1% Control group 1 75 heads 49 heads 26 heads 34.7% 65.3% Control group 2 70 heads 64 heads 6 heads 8.6% 91.4%

[0128] As shown in Tables 1-3, from the clinical manifestations and data of the experimental group and control group 1 and control group 2, the experimental group after the nursery pigs were treated with the drug was significantly better than the control group 1 in terms of body temperature recovery, feed intake changes and final treatment efficacy of the diseased pigs. The body temperature of the experimental group basically returned to normal within 24 hours, and there was no recurrence within 7 days of medication. After the body temperature returned to normal, the feed intake of the nursery pigs increased significantly, and the final cure rate was significantly higher than that of the control group 1. In addition, the experimental group after the nursery pigs were treated with the drug also had obvious advantages over the control group 2 in terms of body temperature recovery, feed intake changes and final treatment efficacy of the diseased pigs. It can be seen that the Bistort Green Tiger Granules are prepared on the basis of Bistort, Terminalia chebula, Polygonum cuspidatum, and Isatis indigotica as raw materials, and then prepared with appropriate amounts of chrysanthemum and Eucommia ulmoides leaves, and the filtrate residues are finally extracted by ultra-high pressure with ethanol / ethyl acetate mixture during the preparation process, which will make it more effective in treating blue ear disease.

[0129] The treatment effect of the experimental group after adding the Quanshen Qinghu Granule in Example 1 was significantly better than that of the control group 1. In addition, after 7 consecutive days of medication, the experimental group did not use drug treatment and was routinely raised for 10 days, and the positive rate of nursery pigs in the experimental group was detected to be 0%. This shows that the Quanshen Qinghu Granule in Example 1 can effectively treat nursery pig respiratory syndrome caused by blue ear disease virus.

[0130] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that the technical solutions described in the aforementioned embodiments may still be modified, or some of the technical features may be replaced by equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A method for preparing Bistorta Qinghu Granules for treating blue ear disease in pigs, characterized in that: The following steps are involved: S1, taking dried scutellaria baicalensis, terminalia chebula, knotweed, isatis indigo plant, chrysanthemum, and eucommia leaf and grinding them to obtain a mixed crushed material; The mass ratio of the ginseng, isatis indigo leaf, polygonum cuspidatum, terminalia chebula, chrysanthemum foetida, and eucommia leaf is 300-450:250-400:80-130:150-250:150-300:200-500; S2, placing the mixed crushed material in an ethanol / water mixture, first performing ultrasonic extraction, and then performing ultrahigh pressure extraction; then filtering to obtain a filtrate 1 and a filter residue 1; decompressing and concentrating the filtrate 1 to obtain a concentrate 1; the power of the ultrasonic extraction is 300-450W, and the time is 35-45min; the pressure of the ultrahigh pressure extraction is 300-450MPa, and the time is 5-7min; S3, placing the filter residue 1 in water, and then performing ultrahigh pressure extraction; filtering to obtain filtrate 2 and filter residue 2; decompressing and concentrating the filtrate 2 to obtain concentrated solution 2; the pressure of the ultrahigh pressure extraction is 400-500 MPa, and the time is 2-3 min; in step S4, the pressure of the ultrahigh pressure extraction is 300-400 MPa, and the time is 2-3 min; S4, adding ethanol and then ethyl acetate to the filter residue 2, and then performing ultra-high pressure extraction; filtering to obtain filtrate 3 and filter residue 3; concentrating and drying the filtrate 3 under reduced pressure to obtain an extract; S5. After the concentrated solution 1 and the concentrated solution 2 are mixed, the extract and auxiliary materials are added, the mixture is evenly mixed, and the Polygonum multiflorum Qinghu Granules are obtained by granulation.

2. The method for preparing the Polysaccharide Resistant Piglets according to claim 1, characterized in that: The mass ratio of the Bistorta, Folium Isatidis, Polygonum cuspidatum, Fructus Terminaliae Terminaliae, Chrysanthemum morifolium and Folium Eucommia ulmoides is 400:300:100:200:200:

300.

3. The method for preparing the Polysaccharide Resistant Piglets according to claim 1, characterized in that: In step S1, the particle size of the mixed crushed material is less than 50 mesh.

4. The method for preparing the Polysaccharide Resistant Piglets according to claim 1, characterized in that: In step S2, the mass volume ratio of the mixed crushed material to the ethanol / water mixed liquid is 1 kg: 6-8 L, the ethanol / water mixed liquid is prepared by mixing ethanol and water, and the volume fraction of ethanol in the ethanol / water mixed liquid is 60-70%; In step S3, the mass volume ratio of the filter residue to water is 1 kg: 3.5-4.5 L; In step S4, the mass volume ratio of the filter residue to ethanol and ethyl acetate is 1 kg: 1-1.5 L: 2.5-3 L.

5. The method for preparing the Polysaccharide Resistant Piglets according to claim 1, characterized in that: In step S2, the temperature during the reduced pressure concentration is 60-65°C, the vacuum degree is -0.08-0.06Mpa, and the relative density of the concentrated solution 1 is 1.05-1.10; in step S3, the temperature during the reduced pressure concentration is 70-75°C, the vacuum degree is -0.08-0.06Mpa, and the relative density of the concentrated solution 2 is 1.05-1.10; in step S4, the temperature during the reduced pressure concentration is 55-60°C, and the vacuum degree is -0.08-0.06Mpa.

6. The method for preparing the Polysaccharide Resistant Piglets according to any one of claims 1 to 5, characterized in that: The auxiliary materials include beta-cyclodextrin, microcrystalline cellulose and sucrose, and the mass ratio of the beta-cyclodextrin, microcrystalline cellulose and sucrose is 1:0.5 to 1:2 to 2.

5.

7. The method for preparing the Polysaccharide Resistant Piglets according to any one of claims 1 to 5, characterized in that: Step S5 specifically comprises the following steps: mixing the concentrated solution 1 and the concentrated solution 2 to obtain a mixed concentrated solution; adding the extract and auxiliary materials accounting for 15-19% of the mass of the mixed concentrated solution to the mixed concentrated solution, mixing them evenly, and spraying and granulating to obtain the Bistort Green Tiger Granules; The inlet air temperature of the spray granulation is 120-125°C, and the outlet air temperature is 75-80°C.