A fixed white rot fungus bioreactor and a method for treating pharmaceutical wastewater using the same

Abstract

The present invention discloses a fixed white rot fungus bioreactor and a method for treating pharmaceutical wastewater using the same, and belongs to the technical field of pharmaceutical wastewater treatment. The fixed white rot fungus bioreactor provided by the present invention is provided with a packing layer, a filter material layer, an aeration layer and a drainage layer from top to bottom, a water distribution pipe is provided on the top of the packing layer, and a water outlet pipe is provided on the side wall of the drainage layer; the packing layer is used to carry white rot fungi, and the packing of the packing layer is a granular packing, which is obtained by mixing sawdust, copper and iron filings and adhesives, pressing into granules, and drying; the drainage layer is composed of a drainage ditch cast by concrete, and the drainage ditch is connected to the water outlet pipe. Through the joint action of the packing layer, the filter material layer, the aeration layer and the drainage layer, it can ensure long-term stable operation, high pharmaceutical wastewater treatment efficiency and good wastewater treatment effect, without the need for frequent packing replacement.

Description

Technical Field

[0001] The invention relates to the technical field of pharmaceutical wastewater treatment, in particular to a fixed white rot fungus bioreactor and a method for treating pharmaceutical wastewater using the same. Background Art

[0002] The information disclosed in the background of the invention is only intended to enhance the understanding of the overall background of the invention and should not be necessarily regarded as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to a person skilled in the art.

[0003] The pollutant composition of pharmaceutical wastewater, especially that of CDMO (Contract Research, Development and Production Organization) projects, is complex and changeable. The organic solvents used in the process have a stable structure and are not easily degraded by microorganisms. Therefore, pharmaceutical wastewater generally has the characteristics of high salt, high toxicity and difficulty in degradation. Traditional treatment methods are mostly to use physical and chemical means to deeply treat pharmaceutical wastewater in order to completely oxidize organic matter and achieve the purpose of meeting the discharge standards of COD (chemical oxygen demand).

[0004] White rot fungi are a type of fungus that has the ability to secrete lignin peroxidase, manganese peroxidase and laccase, so it has a wide range of substrates. Studies have shown that white rot fungi have the ability to degrade aromatic compounds, such as polycyclic aromatic hydrocarbons, chlorinated aromatic compounds, dioxins, nitrobenzene and phenol. In addition, white rot fungi can directly convert organic matter into inorganic substances such as carbon dioxide and water by secreting a variety of degradation enzymes, thereby achieving complete mineralization of organic matter. In view of the complex and changeable composition of pharmaceutical wastewater, the variety of organic matter and its toxicity, white rot fungi can effectively and stably treat pharmaceutical wastewater.

[0005] The patent with publication number CN101549936A uses iron wire and copper wire wrapped corn stalks as carriers for white rot fungi to attach. The carriers absorb organic matter and then stimulate the white rot fungi to release ligninase, thereby degrading organic matter in sewage. However, the structure of such carriers is loose, and broken straw fragments are very easy to block pipes, and will cause the concentration of suspended solids (SS) in wastewater to increase, increasing the difficulty of sewage treatment. Moreover, after the wastewater is treated, it is necessary to recover the sediment and re-cultivate the strains, which is frequent and not conducive to industrial application.

[0006] Therefore, how to provide a bioreactor for fixing white rot fungi with a long operating time, high pharmaceutical wastewater treatment efficiency and good COD removal effect is an urgent problem to be solved. Summary of the invention

[0007] In view of this, the present invention provides a fixed white rot fungus bioreactor and a method for treating pharmaceutical wastewater using the same, which solves the problems of frequent recovery operation of white rot fungus carriers, low treatment efficiency and poor pharmaceutical wastewater treatment effect in the prior art.

[0008] In a first aspect, the present invention provides a fixed white rot fungus bioreactor, which is provided with a packing layer, a filter material layer, an aeration layer and a drainage layer from top to bottom, a water distribution pipe is provided on the top of the packing layer, and a water outlet pipe is provided on the side wall of the drainage layer;

[0009] The packing layer is used to carry white rot fungi. The packing of the packing layer is a granular packing obtained by mixing sawdust, copper and iron filings and adhesive, pressing into granules and drying. The drainage layer is composed of a drainage ditch cast in concrete, and the drainage ditch is connected to a water outlet pipe.

[0010] Preferably, the tank wall material of the fixed white rot fungus bioreactor is steel plate, the bottom base material is concrete, and the drainage layer is arranged on the bottom base; the drainage ditch is provided with 6 to 10 flow channels, multiple flow channels are arranged in parallel, and each flow channel is connected to the outlet pipe.

[0011] Preferably, the volume of the packing layer is 50-70% of the effective volume of the fixed white rot fungus bioreactor tank; the heights of the filter layer, aeration layer and drainage layer are 3-10%, 3-10% and 3-10% of the height of the fixed white rot fungus bioreactor tank, respectively.

[0012] Preferably, the filter material layer is composed of stacked pebble filter materials.

[0013] Preferably, the aeration layer is composed of filter bricks and aeration pipes, the aeration pipes are laid below the filter bricks, and the aeration direction of the aeration pipes is vertically upward.

[0014] Preferably, the filler layer comprises 50-70wt% of wood chips, 10-20wt% of copper and iron filings and 20-30wt% of an adhesive; the raw materials of the adhesive comprise calcium carbonate powder, calcium oxide powder, polyacrylamide, polyvinyl acetate emulsion and water, wherein the mass ratio of calcium carbonate powder, calcium oxide powder, polyacrylamide and polyvinyl acetate emulsion is (30-40):(20-30):(15-20):(20-25), and the mass percentage of polyacrylamide to water is 2-5%.

[0015] Furthermore, the preparation method of the filler of the filler layer is: add washed and drained sawdust, copper and iron filings, polyvinyl acetate emulsion, calcium carbonate powder and calcium oxide powder to the polyacrylamide aqueous solution in sequence, mix them evenly to make filler mud embryos, send the filler mud embryos to a molding machine for pressing and granulation to obtain granular mud embryos, and then dry the granular mud embryos at 150~220℃ to form them.

[0016] Furthermore, the size of the pressed granules is 2-5 mm.

[0017] In a second aspect, the present invention provides a method for treating pharmaceutical wastewater using the above-mentioned fixed white rot fungus bioreactor, comprising the following steps:

[0018] Cultivating and domesticating white rot fungi to obtain white rot fungi liquid;

[0019] Inoculating the white rot fungus liquid into the fixed white rot fungus bioreactor, then introducing clean water or treated pharmaceutical wastewater, and circulating the mixture for more than 72 hours;

[0020] The pharmaceutical wastewater is treated in an anaerobic reactor and then transferred to the fixed white rot fungus bioreactor for a residence time of 3 to 7 days, and then discharged to an aeration tank for further treatment.

[0021] Preferably, the preparation method of the white rot fungus liquid is: prepare a liquid culture medium, then sterilize the liquid culture medium at 105-110°C for 20-40min, then load it into a shaking bottle in batches, adjust the pH to 7-8, inoculate 8-12%vol of white rot fungus seed liquid, adjust the shaking table temperature to 34-36°C, the rotation speed to 150-200rpm, and culture at a constant temperature for 5-7d.

[0022] Preferably, the solvent of the liquid culture medium is water, which includes, by mass fraction, 4-6% glucose, 15-25% bean sprout juice or potato juice, 0.1-0.3% sodium nitrate, 0.1-0.3% ammonium chloride, 0.2-0.4% potassium dihydrogen phosphate, 0.4-0.6% sodium bicarbonate, 0.02-0.04% magnesium sulfate and 0.01-0.03% calcium carbonate.

[0023] Compared with the prior art, the present invention has achieved the following beneficial effects:

[0024] The fixed white rot fungus bioreactor provided by the present invention is provided with a packing layer, a filter material layer, an aeration layer and a drainage layer from top to bottom. The packing of the packing layer can effectively absorb pollutants in wastewater and effectively fix white rot fungi, thereby preventing the loss of white rot fungi; the filter material layer can effectively support the packing layer and filter the broken packing in the metabolic process of the white rot fungi, thereby preventing the aeration layer and the drainage layer from being blocked, and preventing the concentration of suspended solids in the effluent from increasing; the aeration layer provides dissolved oxygen for aerobic microorganisms in the packing layer; the drainage layer can not only play a role of stable support, but also can discharge the effluent in time, thereby achieving effective anti-blocking; through the joint action of each layer, it can be ensured that the fixed white rot fungus bioreactor of the present invention can achieve long-term stable operation, high pharmaceutical wastewater treatment efficiency and good pharmaceutical wastewater treatment effect, without the need for frequent packing replacement. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] The drawings constituting part of the present invention are used to provide a further understanding of the present invention. The exemplary embodiments of the present invention and their description are used to explain the present invention and do not constitute an improper limitation of the present invention. Obviously, for those skilled in the art, other drawings can be obtained based on these drawings without creative work.

[0026] Figure 1 Schematic diagram of the structure of the fixed white rot fungus bioreactor of Example 1 of the present invention;

[0027] Figure 2 is a process flow chart of pharmaceutical wastewater treatment according to Embodiment 1 and Embodiment 2 of the present invention;

[0028] Figure 3 is a process flow chart of pharmaceutical wastewater treatment according to Embodiment 3 and Embodiment 4 of the present invention;

[0029] Among them, 1. packing layer; 2. filter material layer; 3. aeration layer; 4. drainage layer; 5. water inlet pipe; 6. water distributor; 7. outlet pipe; 8. aeration pipeline; 9. drainage ditch; 10. concrete base; 11. fixed white rot fungus bioreactor; 12. IC anaerobic reactor; 13. AO aeration tank; 14. UASB anaerobic reactor; 15. ozone catalytic device. DETAILED DESCRIPTION

[0030] It should be noted that the following detailed descriptions are exemplary and are intended to provide further explanation of the present invention. Unless otherwise specified, all technical and scientific terms used herein have the same meanings as those commonly understood by those skilled in the art to which the present invention belongs.

[0031] The present invention provides a fixed white rot fungus bioreactor, which is provided with a packing layer, a filter material layer, an aeration layer and a drainage layer from top to bottom, a water distribution pipe is provided on the top of the packing layer, and a water outlet pipe is provided on the side wall of the drainage layer;

[0032] The packing layer is used to carry white rot fungi. The packing of the packing layer is a granular packing obtained by mixing sawdust, copper and iron filings and adhesive, pressing into granules and drying. The drainage layer is composed of a drainage ditch cast in concrete, and the drainage ditch is connected to a water outlet pipe.

[0033] In the present invention, pharmaceutical wastewater enters the reactor from the water inlet pipe at the top, is evenly dispersed on the filler through the water distributor, and gradually penetrates downward. When the wastewater flows through the filler, the organic matter in the wastewater is adsorbed by the granular filler of the filler layer, and the sawdust in the filler can be used as a carbon source for the growth of white rot fungi. The white rot fungi secrete a variety of biological enzymes such as laccase and manganese peroxidase. The biological enzymes react with the organic matter absorbed by the filler to decompose and destroy the polycyclic aromatic hydrocarbons such as aniline and aromatic hydrocarbons that are difficult to degrade in the pharmaceutical wastewater. At the same time, the sawdust is loose and porous, and can effectively fix the white rot fungi. Copper and iron filings can provide copper and iron trace elements for white rot fungi, and can also realize waste utilization. The use of sawdust and copper and iron filings can greatly reduce the operating cost of the reactor. The bonding effect of the adhesive, the pressing granulation and the drying together avoid the loose disintegration of the filler, which can not only ensure that the fixed white rot fungi bioreactor has a long operating time, but also avoid the disintegration of the filler leading to an increase in the concentration of suspended solids (SS) in the wastewater, thereby avoiding the burden of additional sewage treatment.

[0034] The present invention also provides a drainage layer composed of a concrete-cast drainage ditch. On the one hand, the concrete structure can effectively support the filler layer, the filter material layer and the aeration layer. On the other hand, the drainage ditch has a wider flow channel, which can discharge the filler debris in time. The drainage ditch of the present invention is provided with 6 to 10 flow channels, and the multiple flow channels are arranged in parallel. Each flow channel is connected to the outlet pipe. The multi-flow channel concrete design can effectively prevent debris from clogging the aeration layer, ensuring good aeration and sufficient dissolved oxygen in the reactor. The multi-flow channel drainage ditch can also be used as a backwash water distribution pipeline, which plays a role in uniform water distribution when backwashing the equipment with water.

[0035] In the present invention, the tank wall material of the fixed white rot fungus bioreactor is steel plate, the bottom base material is concrete, and the drainage layer is arranged on the bottom base. When preparing the fixed white rot fungus bioreactor of the present invention, a concrete base is poured under the hollow steel plate tank wall, so that the bottom of the steel plate tank wall is buried in the concrete base, and then a concrete drainage ditch is poured above the concrete base. The above design is conducive to the construction of the drainage layer.

[0036] In the present invention, the volume of the packing layer is 50-70% of the effective volume of the fixed white rot fungus bioreactor tank, which ensures that the white rot fungi in the packing layer can fully exert their degradation effect and ensures that the packing and wastewater are fully mixed.

[0037] During the metabolism of white rot fungi, fillers are continuously consumed. The filter material layer of the present invention is composed of stacked pebble filter materials, which can not only effectively support the filler layer, but also effectively filter the broken fillers, thereby preventing the aeration layer and the drainage layer from being blocked and affecting the treatment efficiency of the reactor, and also preventing the increase of the suspended solid concentration (SS) in the effluent.

[0038] The aeration layer of the present invention is composed of filter bricks and aeration pipes. The filter bricks can also play a supporting and filtering role. At the same time, the filter bricks have a porous structure, allowing air to pass through in reverse flow. An aeration pipe is laid under the filter bricks. The aeration direction of the aeration pipe is vertically upward. The air rises against the water flow, passes through the filter bricks and the filter material layer to reach the packing layer, ensuring that oxygen can be fully dissolved and continuously providing dissolved oxygen for aerobic microorganisms in the packing layer. The aeration pipe is connected to an external aeration fan.

[0039] The filler of the packing layer of the present invention includes 50-70wt% of sawdust, 10-20wt% of copper and iron filings, and 20-30wt% of adhesive. Sawdust, as the main substance, can provide nutrients required for the growth of white rot fungi and can attach and fix the white rot fungi. Copper and iron filings, as auxiliary materials, provide trace elements required for the growth of white rot fungi. The present invention does not impose any special restrictions on the specific structural morphology of the copper and iron filings, for example, they can be regular or irregular fragments, broken wires, etc.; the present invention does not impose any special restrictions on the mass ratio of copper to iron in the copper and iron filings, which is preferably 1: (0.2-5), and more preferably 1: (0.5-2).

[0040] In the present invention, the adhesive mainly plays a bonding role, and the wood chips and copper and iron filings are bonded and formed. The raw materials of the adhesive include calcium carbonate powder, calcium oxide powder, polyacrylamide, polyvinyl acetate emulsion and water, wherein the mass ratio of calcium carbonate powder, calcium oxide powder, polyacrylamide and polyvinyl acetate emulsion is (30-40): (20-30): (15-20): (20-25), and the mass percentage of polyacrylamide to water is 2-5%. The preparation method of the granular filler of the filler layer of the present invention is: sequentially add the washed and drained wood chips, copper and iron filings, polyvinyl acetate emulsion, calcium carbonate powder and calcium oxide powder to the polyacrylamide aqueous solution, mix them evenly to form a filler mud embryo, send the filler mud embryo to a molding machine for pressing and granulation to obtain a granular mud embryo, and then dry the granular mud embryo at 150-220°C to form it. In the present invention, calcium carbonate, calcium oxide, polyacrylamide and polyvinyl acetate can all play a good bonding role, wherein calcium oxide can react with water in an exothermic reaction to generate lime milk, so that the various substances can form mud embryos after mixing, which is beneficial to the bonding and granulation process. On the one hand, calcium carbonate can play an effective bonding role in the preparation of granular fillers, and on the other hand, it can also serve as a partial carbon source for white rot fungi during the operation of the reactor. Polyacrylamide has an amide bond, which can play a good bonding performance through hydrogen bonding and electrostatic interaction; since the filler is pressed and granulated, polyacrylamide interacts with other bonding materials, and the granular filler in the reactor has a high filling ratio, the loss ratio of polyacrylamide is very low, which can maintain the integrity of the granular filler on the one hand, and on the other hand, a small amount of loss helps to avoid the compaction of the filler. Polyvinyl acetate contains more polar groups, so it can also effectively play a bonding role, and it and polyacrylamide have good biodegradability and little impact on the environment. Through the mutual coordination of the components in the adhesive and the above-mentioned preparation method, not only can the copper and iron filings and sawdust be tightly bonded, but the prepared filler has the advantages of high strength, not easy to break and compact, porous, and easy for microorganisms to attach, and can effectively fix white rot fungi; it also has the advantage of not being easy to harden, so that the reactor can achieve long-term operation without frequent replacement of fillers.

[0041] The present invention does not impose any special restrictions on the size of the pressed granules, and the present invention preferably has a size of 2 to 5 cm. The present invention does not impose any special restrictions on the solid content of the polyvinyl acetate emulsion, and the solid content commonly used in the art can be used, and the present invention preferably has a size of 40 to 60 wt%, and more preferably has a size of 50 to 60 wt%.

[0042] The present invention does not impose any special restrictions on the heights of the filter layer, aeration layer and drainage layer. Preferably, the heights of the filter layer, aeration layer and drainage layer are 3-10%, 3-10% and 3-10% of the height of the fixed white rot fungus bioreactor tank, respectively.

[0043] The present invention also provides a method for treating pharmaceutical wastewater using the fixed white rot fungus bioreactor, comprising the following steps:

[0044] Cultivating and domesticating white rot fungi to obtain white rot fungi liquid;

[0045] Inoculating the white rot fungus liquid into the fixed white rot fungus bioreactor, then introducing clean water or treated pharmaceutical wastewater, and circulating the mixture for more than 72 hours;

[0046] The pharmaceutical wastewater is treated in an anaerobic reactor and then transferred to the fixed white rot fungus bioreactor for a residence time of 3 to 7 days, and then discharged to an aeration tank for further treatment.

[0047] In the present invention, pharmaceutical wastewater first enters the anaerobic reactor, and the easily biodegradable small-molecule organic matter is converted into biogas in the anaerobic reactor, and the non-degradable macromolecular organic matter and polycyclic aromatic hydrocarbons and other substances enter the fixed white-rot fungus bioreactor with the effluent. The macromolecular organic matter and polycyclic aromatic hydrocarbon organic matter adsorbed by the filler inside the fixed white-rot fungus bioreactor are decomposed into small-molecule organic matter under the catalytic action of laccase II manganese peroxidase secreted by the white-rot fungus, and the small-molecule organic matter is absorbed by a variety of aerobic microorganisms and converted into water and carbon dioxide, thereby realizing the mineralization of pollutants. The small-molecule organic matter that does not have time to be completely mineralized in the fixed white-rot fungus bioreactor can be used as a carbon source for denitrification in the aeration tank. After the wastewater treated by the fixed white-rot fungus bioreactor is completely oxidized, ammoniated and denitrified in the aeration tank, the COD value can be reduced to below 300 mg / L.

[0048] The preparation method of the white rot fungus liquid of the present invention comprises the following steps: preparing a liquid culture medium, sterilizing the liquid culture medium at 105-110°C for 20-40 minutes, then loading the liquid culture medium into a shaking bottle in batches, adjusting the pH value to 7-8, inoculating 8-12%vol of white rot fungus seed liquid, adjusting the shaking table temperature to 34-36°C, the rotation speed to 150-200rpm, and culturing at a constant temperature for 5-7d to obtain the liquid.

[0049] In the present invention, the solvent of the liquid culture medium is water, which comprises, by mass fraction, 4-6% glucose, 15-25% bean sprout juice or potato juice, 0.1-0.3% sodium nitrate, 0.1-0.3% ammonium chloride, 0.2-0.4% potassium dihydrogen phosphate, 0.4-0.6% sodium bicarbonate, 0.02-0.04% magnesium sulfate and 0.01-0.03% calcium carbonate.

[0050] The technical solution of the present invention is further described below in conjunction with specific embodiments.

[0051] Example 1

[0052] This embodiment provides a method for treating pharmaceutical wastewater using a fixed white rot fungus bioreactor 11. The structural schematic diagram of the fixed white rot fungus bioreactor 11 is shown in FIG. Figure 1 As shown in the figure, the process flow chart of pharmaceutical wastewater treatment is as follows Figure 2 shown.

[0053] The diameter of the fixed white rot fungus bioreactor 11 is 6m and the height is 10m. It is provided with a packing layer 1, a filter material layer 2, an aeration layer 3 and a drainage layer 4 from top to bottom. The top of the packing layer 1 is provided with a water inlet pipe 5 and a water distributor 6. The side wall of the drainage layer 4 is provided with a water outlet pipe 7. The drainage layer 4 is provided on a concrete base 10, and the tank wall is made of a steel plate. The filter material layer 2 is formed by stacking pebble filter materials, and the aeration layer 3 includes filter bricks and aeration pipes 8. The aeration pipes 8 are below the filter bricks. The drainage layer 4 is composed of a drainage ditch 9 cast in concrete, and the drainage ditch 9 is connected to the water outlet pipe 7. The height of the packing layer 1 is 5m, and the heights of the filter material layer 2, the aeration layer 3 and the drainage layer 4 are 0.8m, 0.6m and 0.5m respectively.

[0054] In this embodiment, the filler of the filler layer 1 includes 60wt% sawdust, 15wt% copper and iron filings (the mass ratio of copper to iron is 1:1) and 25wt% adhesive. Among the raw materials of the adhesive, the mass ratio of calcium carbonate powder, calcium oxide powder, polyacrylamide and polyvinyl acetate emulsion (solid content is 55wt%) is 35:25:20:20. The filler preparation process is as follows: the collected sawdust is screened to remove the broken pieces, then washed with clean water and soaked for 2h, drained and set aside; the drained sawdust and copper and iron filings are added to the polyacrylamide aqueous solution with a concentration of 3wt%, then polyvinyl acetate emulsion and calcium carbonate powder are added, and finally calcium oxide powder is added, mixed evenly to form a filler mud embryo, which is sent to a molding machine for pressing and granulation, and the granulation size is about 3cm; the granular mud embryo is dried and formed at 200℃ in a dryer.

[0055] The preparation method of high-concentration white rot fungus liquid is as follows: first prepare a liquid culture medium, which includes 5% glucose, 20% potato juice, 0.2% sodium nitrate, 0.2% ammonium chloride, 0.3% potassium dihydrogen phosphate, 0.5% sodium bicarbonate, 0.03% magnesium sulfate, 0.02% calcium carbonate, and the rest is water. After the liquid culture medium is successfully prepared, sterilize it at 108°C for 30 minutes. The sterilized liquid culture medium is loaded into a shaking bottle in batches, the pH is adjusted to 7, 10% vol of white rot fungus seed liquid is inoculated, the shaking table temperature is adjusted to 35°C, the speed is 180rpm, and the constant temperature culture is carried out for 6 days to obtain a high-concentration white rot fungus liquid.

[0056] After the pharmaceutical wastewater is regulated in the regulating tank, the pH is 6, the temperature is 30°C, and the COD is 12000mg / L. After the fixed white rot fungus bioreactor 11 is filled and tested, 20%vol of clean water is first introduced, and then the above-mentioned high-concentration white rot fungus liquid is inoculated into the fixed white rot fungus bioreactor 11, and the internal circulation is 75h to ensure that the filler fully adsorbs the bacteria. The regulated pharmaceutical wastewater first enters the IC anaerobic reactor 12, and the COD value of the pharmaceutical wastewater is reduced to 6000mg / L after treatment. Then the treated pharmaceutical wastewater enters the fixed white rot fungus bioreactor 11 inoculated with white rot fungi, and the residence time is 3 days. The COD value of the treated wastewater is reduced to 600mg / L. Then the wastewater enters the AO aeration tank 13. After thorough oxidation, ammoniation and denitrification in the aeration tank, the COD in the wastewater is reduced to 200mg / L, meeting the local emission standards.

[0057] Example 2

[0058] This embodiment provides a method for treating pharmaceutical wastewater using a fixed white rot fungus bioreactor 11. The process flow chart is as follows: Figure 2 shown.

[0059] After the pharmaceutical wastewater is adjusted in the regulating tank, the pH is 6.5, the temperature is 30℃, and the COD is 12000mg / L.

[0060] The structure of the fixed white rot fungus bioreactor 11 in this embodiment is different from that in Example 1, the diameter of the fixed white rot fungus bioreactor 11 in this embodiment is 5m, the height is 10m, the height of the packing layer 1 is 6.5m, and the heights of the filter material layer 2, the aeration layer 3 and the drainage layer 4 are 0.5m, 0.5m and 0.5m respectively. After the fixed white rot fungus bioreactor 11 is filled and tested, 20%vol of clean water is first introduced, and then the high-concentration white rot fungus liquid of Example 1 is inoculated into the fixed white rot fungus bioreactor 11, and the internal circulation is 80h to ensure that the filler fully adsorbs the bacteria.

[0061] The regulated pharmaceutical wastewater first enters the IC anaerobic reactor 12, and the COD value of the pharmaceutical wastewater is reduced to 6000 mg / L after treatment. Then the treated pharmaceutical wastewater enters the fixed white rot fungus bioreactor 11 inoculated with white rot fungi, and the residence time is 3 days. The COD value of the treated wastewater is reduced to 530 mg / L. Then the wastewater enters the AO aeration tank 13, and after thorough oxidation, ammoniation and denitrification in the aeration tank, the COD in the wastewater is reduced to 160 mg / L, meeting the local emission standards.

[0062] Example 3

[0063] This embodiment provides a method for treating pharmaceutical wastewater using a fixed white rot fungus bioreactor 11. The process flow chart is as follows: Figure 3 shown.

[0064] After the pharmaceutical wastewater is adjusted in the regulating tank, the pH is 6.5, the temperature is 30℃, and the COD is 16000mg / L.

[0065] The structure of the fixed white rot fungus bioreactor 11 in this embodiment is different from that in Example 1, the diameter of the fixed white rot fungus bioreactor 11 in this embodiment is 8m, the height is 10m, the height of the packing layer 1 is 5.8m, and the heights of the filter material layer 2, the aeration layer 3 and the drainage layer 4 are 0.7m, 0.6m and 0.5m respectively. After the fixed white rot fungus bioreactor 11 is filled and tested, 20%vol of treated pharmaceutical wastewater (COD<200mg / L) is first introduced, and then the high-concentration white rot fungus liquid of Example 1 is inoculated into the fixed white rot fungus bioreactor 11, and the internal circulation is 80h to ensure that the filler fully adsorbs the bacteria.

[0066] The regulated pharmaceutical wastewater first enters the UASB anaerobic reactor 14, and the COD value of the pharmaceutical wastewater is reduced to 11000 mg / L after treatment. Then the treated pharmaceutical wastewater enters the fixed white rot fungus bioreactor 11 inoculated with white rot fungi, and the residence time is 4.5 days. The COD value of the treated wastewater is reduced to 800 mg / L. Then the wastewater enters the AO aeration tank 13, and after thorough oxidation, ammoniation and denitrification in the AO aeration tank 13, the COD in the wastewater is reduced to 260 mg / L, and then after oxidation treatment by the terminal ozone catalytic device 15, the COD is reduced to 80 mg / L, meeting the national secondary sewage discharge standard.

[0067] Example 4

[0068] This embodiment provides a method for treating pharmaceutical wastewater using a fixed white rot fungus bioreactor 11. The process flow chart is as follows: Figure 3 shown.

[0069] After the pharmaceutical wastewater is adjusted in the regulating tank, the pH is 6.5, the temperature is 30℃, and the COD is 16000mg / L.

[0070] The structure of the fixed white rot fungus bioreactor 11 in this embodiment is different from that in Example 1, the diameter of the fixed white rot fungus bioreactor 11 in this embodiment is 8m, the height is 10m, the height of the packing layer 1 is 6.5m, and the heights of the filter material layer 2, the aeration layer 3 and the drainage layer 4 are 0.5m, 0.5m and 0.5m respectively. After the fixed white rot fungus bioreactor 11 is filled and tested, 20%vol of treated pharmaceutical wastewater (COD<200mg / L) is first introduced, and then the high-concentration white rot fungus liquid of Example 1 is inoculated into the fixed white rot fungus bioreactor 11, and the internal circulation is 80h to ensure that the filler fully adsorbs the bacteria.

[0071] The regulated pharmaceutical wastewater first enters the UASB anaerobic reactor 14, and the COD value of the pharmaceutical wastewater is reduced to 11000 mg / L after treatment. Then the treated pharmaceutical wastewater enters the fixed white rot fungus bioreactor 11 inoculated with white rot fungi, and the residence time is 4.5 days. The COD value of the treated wastewater is reduced to 700 mg / L. Then the wastewater enters the AO aeration tank 13, and after thorough oxidation, ammoniation and denitrification in the AO aeration tank 13, the COD in the wastewater is reduced to 220 mg / L, and then after oxidation treatment by the terminal ozone catalytic device 15, the COD is reduced to 50 mg / L, meeting the national secondary sewage discharge standard.

[0072] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A fixed white rot fungus bioreactor, characterized in that: It is provided with a packing layer, a filter material layer, an aeration layer and a drainage layer from top to bottom. A water distribution pipe is provided on the top of the packing layer, and a water outlet pipe is provided on the side wall of the drainage layer. The volume of the packing layer is 50-70% of the effective volume of the fixed white rot fungus bioreactor tank; the heights of the filter layer, aeration layer and drainage layer are 3-10%, 3-10% and 3-10% of the height of the fixed white rot fungus bioreactor tank, respectively; The filler layer is used to carry white rot fungi, and the filler of the filler layer is a granular filler, which is obtained by mixing sawdust, copper and iron filings and adhesives, pressing into granules, and drying; the drainage layer is composed of a drainage ditch cast in concrete, and the drainage ditch is connected to the outlet pipe; The filler layer comprises 50-70wt% of wood chips, 10-20wt% of copper and iron filings and 20-30wt% of an adhesive; the raw materials of the adhesive comprise calcium carbonate powder, calcium oxide powder, polyacrylamide, polyvinyl acetate emulsion and water, wherein the mass ratio of calcium carbonate powder, calcium oxide powder, polyacrylamide and polyvinyl acetate emulsion is (30-40):(20-30):(15-20):(20-25), and the mass percentage of polyacrylamide to water is 2-5%; The mass ratio of copper to iron in copper-iron filings is 1:(0.2~5).

2. The fixed white rot fungus bioreactor according to claim 1, characterized in that: The tank wall material of the fixed white rot fungus bioreactor is steel plate, the bottom base material is concrete, the drainage layer is arranged on the bottom base; the drainage ditch is provided with 6 to 10 flow channels, the multiple flow channels are arranged in parallel, and each flow channel is connected to the outlet pipe.

3. The fixed white rot fungus bioreactor according to claim 1, characterized in that: The filter material layer is composed of stacked pebble filter materials; the aeration layer is composed of filter bricks and aeration pipes, the aeration pipes are laid under the filter bricks, and the aeration direction of the aeration pipes is vertically upward.

4. The fixed white rot fungus bioreactor according to claim 1, characterized in that: The preparation method of the filler of the filler layer is as follows: wash and drain sawdust, copper and iron filings, polyvinyl acetate emulsion, calcium carbonate powder and calcium oxide powder are sequentially added into the polyacrylamide aqueous solution, mixed evenly to form a filler mud embryo, the filler mud embryo is sent to a molding machine for pressing and granulation to obtain a granular mud embryo, and then the granular mud embryo is dried and formed at 150-220°C.

5. The fixed white rot fungus bioreactor according to claim 4, characterized in that: The size of the pressed granules is 2-5 mm.

6. A method for treating pharmaceutical wastewater using the fixed white rot fungus bioreactor according to any one of claims 1 to 5, characterized in that: The steps include: Cultivating and domesticating white rot fungi to obtain white rot fungi liquid; Inoculating the white rot fungus liquid into the fixed white rot fungus bioreactor, then introducing clean water or treated pharmaceutical wastewater, and circulating the mixture for more than 72 hours; The pharmaceutical wastewater is treated in an anaerobic reactor and then transferred to the fixed white rot fungus bioreactor for a residence time of 3 to 7 days, and then discharged to an aeration tank for further treatment.

7. The method according to claim 6, characterized in that The preparation method of the white rot fungus liquid comprises the following steps: preparing a liquid culture medium, sterilizing the liquid culture medium at 105-110° C. for 20-40 minutes, then loading the liquid culture medium into a shaking bottle in batches, adjusting the pH value to 7-8, inoculating 8-12% vol of a white rot fungus seed liquid, adjusting the shaking table temperature to 34-36° C., the rotation speed to 150-200 rpm, and culturing at a constant temperature for 5-7 days.

8. The method according to claim 7, characterized in that The solvent of the liquid culture medium is water, which comprises, by mass fraction, 4-6% glucose, 15-25% bean sprout juice or potato juice, 0.1-0.3% sodium nitrate, 0.1-0.3% ammonium chloride, 0.2-0.4% potassium dihydrogen phosphate, 0.4-0.6% sodium bicarbonate, 0.02-0.04% magnesium sulfate and 0.01-0.03% calcium carbonate.

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