An anti-aging functional peptide composition and preparation method thereof

Abstract

The present invention discloses an anti-aging functional peptide composition and a preparation method thereof, and relates to the technical field of food health care. The anti-aging functional peptide composition comprises the following components by weight: 1-10 parts of ascorbic acid polypeptide, 10-30 parts of calcium peptide chelate, 1-10 parts of medicinal fasciculus polysaccharide, 10-20 parts of plant extract, 1-10 parts of bird's nest extract, 1-5 parts of sea cucumber peptide, 1-5 parts of chlorella active peptide, 1-10 parts of auxiliary materials and 15-45 parts of water. The present invention adopts the above-mentioned anti-aging functional peptide composition and a preparation method thereof, in which the calcium peptide chelate and the medicinal fasciculus polysaccharide in the anti-aging functional peptide composition cooperate with each other, and are supplemented with other extracts, and multiple components cooperate with each other to effectively remove free radicals, thereby delaying aging.

Description

Technical Field

[0001] The invention relates to the technical field of food health care, and in particular to an anti-aging functional peptide composition and a preparation method thereof. Background Art

[0002] Aging is a spontaneous and inevitable process of organisms over time. It is a complex natural phenomenon, manifested as structural degeneration and functional decline, and reduced adaptability and resistance. Aging is the process of gradual decline in the functions of various organ systems in the body. The final result of aging is death. It is the end of life. There are many factors that affect human aging, such as living environment, lifestyle, mental state, genetic factors, etc. Measures to delay aging include scientific and reasonable life, a relaxed and happy mood, and appropriate entertainment and sports activities. Aging is a natural law that cannot be violated. However, when people adopt good living habits and health care measures and exercise appropriately, they can effectively delay aging, reduce the incidence of aging-related diseases, and improve the quality of life.

[0003] Free radicals refer to electrons, molecules, atoms or ions containing single groups, which are unpaired atoms or groups. Its single electrons have a strong tendency to pair up in order to form a stable structure. Free radicals are intermediate products of various biochemical reactions in human life activities. When excessive oxygen free radicals are produced in the body, they can cause lipid peroxidation and form lipofuscin; excessive oxygen free radicals can also damage cells and their components, such as DNA mutations, protein and enzyme denaturation, etc., thereby accelerating aging.

[0004] In recent years, scientists have discovered through research and exploration that functional peptides in some foods can remove free radicals and achieve the effect of delaying aging. Therefore, the present invention provides an anti-aging functional peptide composition that can effectively remove free radicals in the body, thereby delaying aging. Summary of the invention

[0005] The purpose of the present invention is to provide an anti-aging functional peptide composition and a preparation method thereof. The calcium peptide chelate and medicinal fasciculus polysaccharide in the anti-aging functional peptide composition cooperate with each other and are supplemented with other extracts. The multiple components cooperate with each other to effectively remove free radicals, thereby delaying aging.

[0006] To achieve the above object, the present invention provides an anti-aging functional peptide composition, comprising the following components in parts by weight: 1-10 parts of ascorbic acid polypeptide, 10-30 parts of calcium peptide chelate, 1-10 parts of medicinal fasciculus polysaccharide, 10-20 parts of plant extract, 1-10 parts of bird's nest extract, 1-5 parts of sea cucumber peptide, 1-5 parts of chlorella active peptide, 1-10 parts of auxiliary materials and 15-45 parts of water.

[0007] Further, the following components are included by weight: 5 parts of ascorbic acid polypeptide, 28 parts of calcium peptide chelate, 3 parts of medicinal fusiformis polysaccharide, 18 parts of plant extract, 6 parts of bird's nest extract, 2 parts of sea cucumber peptide, 3 parts of chlorella active peptide, 5 parts of auxiliary materials and 30 parts of water. The molecular weight of sea cucumber peptide and chlorella active peptide is 500-1000 Daltons.

[0008] Furthermore, the preparation method of the calcium peptide chelate is as follows:

[0009] Fish collagen peptide and bone powder were mixed in a mass ratio of 2-3:1, the pH was adjusted to 7, and the mixture was placed in a constant temperature water bath at 60°C for 1.5-3h. Then, 6 times the volume of pure ethanol was added for precipitation, and the mixture was filtered and freeze-dried to obtain a calcium peptide chelate.

[0010] Furthermore, the molecular weight of the fish collagen peptide is 1000-2000 Daltons, and the preparation method is as follows:

[0011] 1) Pretreatment: deash and defat the fish scales, then wash them with deionized water and cut them into pieces for later use;

[0012] 2) Initial enzymatic hydrolysis: put the pre-treated fish scales into a reaction tank, add enzymatic hydrolyzing agent, react for a period of time, filter, and obtain a collagen peptide enzymatic hydrolyzate;

[0013] 3) secondary enzymatic hydrolysis, grinding the fish scales obtained by filtration in step 2) into powder, adding an enzymatic hydrolyzing agent, reacting for a period of time, filtering, and obtaining a secondary collagen peptide enzymatic hydrolyzate;

[0014] 4) Filtration: filtering the primary collagen peptide hydrolysate and the secondary collagen peptide hydrolysate to obtain a fish collagen peptide solution.

[0015] Furthermore, in step 1), the fish scale fragments are 0.25-0.5 cm 2 fragments; in step 2), the enzymatic agent is protease, and the protease accounts for 3-8% of the total weight of the fish scales; in step 3), the enzymatic agent is protease, and the protease accounts for 3-5% of the total weight of the fish scales; in step 4), the fish collagen peptide solution is filtered twice.

[0016] Furthermore, the bone meal includes any one of fish bones, pig bones, sheep bones, and cattle bones, or a combination of several of them.

[0017] Furthermore, the preparation method of medicinal fasciatus polysaccharide is as follows:

[0018] The medicinal fasciculus was inoculated into a liquid fermentation medium and cultured at 28 ℃ and 180 r / min for 4.5 d. The mycelium of the medicinal fasciculus was filtered and freeze-dried. The fruiting bodies of the medicinal fasciculus were naturally air-dried, crushed and dried to a constant mass. Then, 50 g of mycelium freeze-dried powder and fruiting body dried powder were taken respectively, distilled water was added at a solid-liquid ratio of 1:25 m / V, and hot water was used for extraction at 85 ℃ for 2 h. This was repeated twice, and the filtrates were combined by suction to obtain a polysaccharide extract.

[0019] Furthermore, the plant extract includes any one of Centella asiatica extract, green tea extract, and Ginkgo biloba extract, or a combination of several of them.

[0020] Furthermore, the auxiliary materials include oligofructose, inulin and starch.

[0021] The present invention also provides a method for preparing the above-mentioned anti-aging functional peptide composition, comprising the following steps:

[0022] Weigh each raw material component according to weight, and then mix them evenly to obtain an anti-aging functional peptide composition solution, and use a vacuum freezer to perform vacuum freeze-drying to obtain an anti-aging functional peptide composition freeze-dried powder.

[0023] The advantages and positive effects of the anti-aging functional peptide composition and the preparation method thereof described in the present invention are:

[0024] 1. The present invention chelates fish collagen peptides and bone powder to obtain calcium peptide chelates, which can effectively remove free radicals and have good antioxidant properties, thereby delaying aging.

[0025] 2. The present invention adds medicinal Familiopsis polysaccharide to the anti-aging composition. The medicinal Familiopsis polysaccharide contains excellent antioxidant substances, which can effectively remove free radicals and prevent oxidation reactions, thereby delaying sun aging.

[0026] 3. The calcium peptide chelate of the present invention and the medicinal polysaccharide of Pseudomonas aeruginosa work synergistically with each other and are supplemented with other extracts. The various components cooperate with each other to effectively remove free radicals, thereby delaying aging.

[0027] The technical solution of the present invention is further described in detail below through embodiments. DETAILED DESCRIPTION

[0028] The technical solution of the present invention is further illustrated by the following examples.

[0029] Unless otherwise defined, technical or scientific terms used in the present invention shall have the common meanings understood by one having ordinary skills in the field to which the present invention belongs.

[0030] Unless otherwise defined, the instruments, equipment, reagents, etc. used in the present invention are all commercially available.

[0031] An anti-aging functional peptide composition comprises the following components in parts by weight: 1-10 parts of ascorbic acid polypeptide, 10-30 parts of calcium peptide chelate, 1-10 parts of medicinal fasciculus polysaccharide, 10-20 parts of plant extract, 1-10 parts of bird's nest extract, 1-5 parts of sea cucumber peptide, 1-5 parts of chlorella active peptide, 1-10 parts of auxiliary materials and 15-45 parts of water.

[0032] The preparation method of the calcium peptide chelate is as follows:

[0033] Fish collagen peptide and bone powder were mixed in a mass ratio of 2-3:1, the pH was adjusted to 7, and the mixture was placed in a constant temperature water bath at 60°C for 1.5-3h. Then, 6 times the volume of pure ethanol was added for precipitation, and the mixture was filtered and freeze-dried to obtain a calcium peptide chelate.

[0034] The molecular weight of fish collagen peptide is 1000-2000 Daltons, and the preparation method is as follows:

[0035] 1) Pretreatment: deash and defat the fish scales, then wash them with deionized water and cut them into pieces for later use;

[0036] 2) Initial enzymatic hydrolysis: put the pre-treated fish scales into a reaction tank, add enzymatic hydrolyzing agent, react for a period of time, filter, and obtain a collagen peptide enzymatic hydrolyzate;

[0037] 3) secondary enzymatic hydrolysis, grinding the fish scales obtained by filtration in step 2) into powder, adding an enzymatic hydrolyzing agent, reacting for a period of time, filtering, and obtaining a secondary collagen peptide enzymatic hydrolyzate;

[0038] 4) Filtration: filtering the primary collagen peptide hydrolysate and the secondary collagen peptide hydrolysate to obtain a fish collagen peptide solution.

[0039] Step 1) The fish scale fragments are 0.25-0.5cm 2 fragments; in step 2), the enzymatic agent is protease, and the protease accounts for 3-8% of the total weight of the fish scales; in step 3), the enzymatic agent is protease, and the protease accounts for 3-5% of the total weight of the fish scales; in step 4), the fish collagen peptide solution is filtered twice.

[0040] The bone meal includes any one of fish bones, pig bones, sheep bones, and cattle bones, or a combination of several of them.

[0041] The preparation method of medicinal fasciculus polysaccharide is as follows:

[0042] The medicinal fasciculus was inoculated into a liquid fermentation medium and cultured at 28 ℃ and 180 r / min for 4.5 d. The mycelium of the medicinal fasciculus was filtered and freeze-dried. The fruiting bodies of the medicinal fasciculus were naturally air-dried, crushed and dried to a constant mass. Then, 50 g of mycelium freeze-dried powder and fruiting body dried powder were taken respectively, distilled water was added at a solid-liquid ratio of 1:25 m / V, and hot water was used for extraction at 85 ℃ for 2 h. This was repeated twice, and the filtrates were combined by suction to obtain a polysaccharide extract.

[0043] The plant extract includes any one of Centella asiatica extract, green tea extract, and ginkgo extract, or a combination of several of them. The molecular weights of sea cucumber peptide and chlorella active peptide are between 500 and 1000 Daltons.

[0044] Example 1

[0045] An anti-aging functional peptide composition A comprises the following components in parts by weight: 5 parts of ascorbic acid polypeptide, 28 parts of calcium peptide chelate, 3 parts of medicinal fasciculus polysaccharide, 18 parts of plant extract, 6 parts of bird's nest extract, 2 parts of sea cucumber peptide, 3 parts of chlorella active peptide, 5 parts of auxiliary materials and 30 parts of water.

[0046] Example 2

[0047] An anti-aging functional peptide composition comprises the following components in parts by weight: 2 parts of ascorbic acid polypeptide, 12 parts of calcium peptide chelate, 2 parts of medicinal facies porphyra polysaccharide, 10 parts of plant extract, 6 parts of bird's nest extract, 1 part of sea cucumber peptide, 2 parts of chlorella active peptide, 3 parts of auxiliary materials and 17 parts of water.

[0048] Example 3

[0049] An anti-aging functional peptide composition comprises the following components in parts by weight: 8 parts of ascorbic acid polypeptide, 28 parts of calcium peptide chelate, 6 parts of medicinal facies porphyra polysaccharide, 19 parts of plant extract, 10 parts of bird's nest extract, 2 parts of sea cucumber peptide, 3 parts of chlorella active peptide, 9 parts of auxiliary materials and 44 parts of water.

[0050] Comparative Example 1

[0051] An anti-aging functional peptide composition comprises the following components in parts by weight: 5 parts of ascorbic acid polypeptide, 28 parts of unchelated fish collagen peptide, 3 parts of medicinal fasciculus polysaccharide, 18 parts of plant extract, 6 parts of bird's nest extract, 2 parts of sea cucumber peptide, 3 parts of chlorella active peptide, 5 parts of auxiliary materials and 30 parts of water.

[0052] Comparative Example 2

[0053] An anti-aging functional peptide composition comprises the following components in parts by weight: 5 parts of ascorbic acid polypeptide, 28 parts of unchelated bone powder, 3 parts of medicinal facies porphyra polysaccharide, 18 parts of plant extract, 6 parts of bird's nest extract, 2 parts of sea cucumber peptide, 3 parts of chlorella active peptide, 5 parts of auxiliary materials and 30 parts of water.

[0054] Comparative Example 3

[0055] An anti-aging functional peptide composition comprises the following components in parts by weight: 5 parts of ascorbic acid polypeptide, 28 parts of calcium peptide chelate, 3 parts of wolfberry polysaccharide, 18 parts of plant extract, 6 parts of bird's nest extract, 2 parts of sea cucumber peptide, 3 parts of chlorella active peptide, 5 parts of auxiliary materials and 30 parts of water.

[0056] Using an in vitro antioxidant experiment, a free radical scavenging experiment was performed on the anti-aging functional peptide compositions prepared in Examples 1, 2, 3 and Comparative Examples 1, 2, 3.

[0057] (1) Hydroxyl free radical scavenging experiment:

[0058] Accurately measure 1 mL of the liquid to be tested with a mass concentration of 0.01 mg / mL in a test tube, add 2 ml of 9 mmol / L FeSO4 solution and salicylic acid-ethanol solution, and 8.8 mmol / L H2O2 solution in turn, dilute to 10 ml with ultrapure water, let stand for 30 min, and measure the absorbance A1 at 510 nm; measure the absorbance A0 of ultrapure water (blank control solution) at 510 nm. At the same time, accurately measure 1 mL of the liquid to be tested with a mass concentration of 0.01 mg / mL in a test tube, first add 2 mL of 9 mmol / L FeSO4 solution, then add 2 mL of 9 mmol / L salicylic acid-ethanol solution, dilute to 10 mL with ultrapure water, and measure the absorbance A2 at 510 nm. Among them, the clearance rate (%) = [1-(A l -A2) / A0]×100%.

[0059] (2) DPPH free radical scavenging experiment:

[0060] Prepare 0.1 mmol / L DPPH solution with anhydrous ethanol and store in the dark. Add 2 mL of the anti-aging functional peptide composition solution prepared in Examples 1-3 and Comparative Examples 1-3 and 2 mL of DPPH solution to the same test tube, shake well, let stand in the dark at room temperature for 30 minutes, and then measure its absorbance A1. At the same time, measure the absorbance A0 after mixing 2 mL of DPPH solution with 2 mL of distilled water. Free radical scavenging ability: DPPH scavenging percentage = (A0-A1) / A0×100. A0 is the absorbance of DPPH at 520 nm when no sample is added; A1 is the absorbance of DPPH at 520 nm after adding the sample.

[0061] The anti-aging functional peptide compositions prepared in Examples 1, 2, 3 and Comparative Examples 1, 2, 3 were prepared with a concentration of 0.01 mg / ml as the test liquid, and the test results are shown in Table 1.

[0062] ;

[0063] It can be seen from Table 1 that compared with Comparative Examples 1, 2 and 3, the compositions prepared in Examples 1, 2 and 3 have stronger antioxidant properties; compared with Comparative Examples 1 and 2, when the unchelated collagen peptide or bone powder is used alone, the antioxidant property of the composition is poor in Example 1, indicating that the antioxidant properties of the chelated collagen peptide and bone powder are significantly enhanced; compared with Comparative Example 3, the antioxidant property of Example 1 is significantly better, indicating that the antioxidant effect of the medicinal Pseudomonas polysaccharide is better than that of Lycium barbarum polysaccharide, and the medicinal Pseudomonas polysaccharide and the chelated collagen peptide synergize with each other, and are supplemented with other extracts, and the various components cooperate with each other, effectively improving the antioxidant property, effectively removing free radicals, and thus delaying aging.

[0064] Therefore, the present invention adopts the above-mentioned anti-aging functional peptide composition and its preparation method. The calcium peptide chelate and medicinal fasciculatum polysaccharide in the anti-aging functional peptide composition work together, and are supplemented with other extracts. The multiple ingredients cooperate with each other to effectively remove free radicals, thereby delaying aging.

[0065] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that they can still modify or replace the technical solution of the present invention with equivalents, and these modifications or equivalent replacements cannot cause the modified technical solution to deviate from the spirit and scope of the technical solution of the present invention.

Claims

1. An anti-aging functional peptide composition, characterized in that: The invention is prepared from the following components in parts by weight: 1-10 parts of ascorbic acid polypeptide, 10-30 parts of calcium peptide chelate, 1-10 parts of medicinal fasciculata polysaccharide, 10-20 parts of plant extract, 1-10 parts of bird's nest extract, 1-5 parts of sea cucumber peptide, 1-5 parts of chlorella active peptide, 1-10 parts of auxiliary materials and 15-45 parts of water; The preparation method of calcium peptide chelate is as follows: The fish collagen peptide and bone powder were mixed in a mass ratio of 2-3:1, the pH was adjusted to 7, and the mixture was placed in a constant temperature water bath at 60°C for 1.5-3 hours, and then 6 times the volume of pure ethanol was added for precipitation, filtered, and freeze-dried to obtain a calcium peptide chelate; The molecular weight of fish collagen peptide is 1000-2000 Daltons, and the preparation method is as follows: 1) Pretreatment: deash and defat the fish scales, then wash them with deionized water and cut them into pieces for later use; 2) Initial enzymatic hydrolysis: put the pre-treated fish scales into a reaction tank, add enzymatic hydrolyzing agent, react for a period of time, filter, and obtain a collagen peptide enzymatic hydrolyzate; 3) secondary enzymatic hydrolysis, grinding the fish scales filtered in step 2) into powder, adding an enzymatic hydrolyzing agent, reacting for a period of time, filtering, and obtaining a secondary collagen peptide enzymatic hydrolyzate; 4) Filtering, filtering the primary collagen peptide hydrolysate and the secondary collagen peptide hydrolysate to obtain a fish collagen peptide solution; The preparation method of medicinal fasciculus polysaccharide is as follows: The medicinal fasciculus was inoculated into a liquid fermentation medium, cultured at 28 ℃ and 180 r / min for 4.5 days, and the mycelium of the medicinal fasciculus was filtered out. The mycelium was freeze-dried, and the fruiting bodies of the medicinal fasciculus were naturally air-dried. After being crushed, they were dried to a constant mass, and then 50 g of mycelium freeze-dried powder and fruiting body dried powder were taken respectively, distilled water was added at a solid-liquid ratio of 1:25 m / V, and hot water was used for extraction at 85 ℃ for 2 h, which was repeated twice, and the filtrates were filtered and combined to obtain a polysaccharide extract. The plant extract includes any one of Centella asiatica extract, green tea extract, and Ginkgo biloba extract, or a combination of several of them.

2. An anti-aging functional peptide composition according to claim 1, characterized in that: The invention is prepared from the following components in parts by weight: 5 parts of ascorbic acid polypeptide, 28 parts of calcium peptide chelate, 3 parts of medicinal fasciculata polysaccharide, 18 parts of plant extract, 6 parts of bird's nest extract, 2 parts of sea cucumber peptide, 3 parts of chlorella active peptide, 5 parts of auxiliary materials and 30 parts of water.

3. The anti-aging functional peptide composition according to claim 1, characterized in that: Step 1) The fish scale fragments are 0.25-0.5cm 2 fragments; in step 2), the enzymatic agent is protease, and the protease accounts for 3-8% of the total weight of the fish scales; in step 3), the enzymatic agent is protease, and the protease accounts for 3-5% of the total weight of the fish scales; in step 4), the fish collagen peptide solution is filtered twice.

4. The anti-aging functional peptide composition according to claim 1, characterized in that: The bone meal includes any one of fish bones, pig bones, sheep bones, and cattle bones, or a combination of several of them.

5. The anti-aging functional peptide composition according to claim 1, characterized in that: The auxiliary materials include oligofructose, inulin and starch.

6. A method for preparing an anti-aging functional peptide composition according to any one of claims 1 to 5, characterized in that: The following steps are involved: Weigh each raw material component according to weight, and then mix them evenly to obtain an anti-aging functional peptide composition solution, and use a vacuum freezer to perform vacuum freeze-drying to obtain an anti-aging functional peptide composition freeze-dried powder.