A moisturizing and anti-wrinkle composition and its application on lips

Abstract

The present invention belongs to the technical field of cosmetics, and specifically relates to a moisturizing and anti-wrinkle composition and its application on lips. The moisturizing and anti-wrinkle composition comprises the following components by mass: 5-9 parts of composition A, 12-15 parts of composition B, 1-4 parts of di-diglycerol polyacyl adipate-2, 1-3 parts of polyglycerol-2 triisostearate, 0.1-8 parts of white meadowfoam seed oil and 0.1-10 parts of argania spinosa kernel oil. The moisturizing and anti-wrinkle composition of the present invention has a good moisturizing and anti-wrinkle effect, and the lip product prepared by the composition is friendly to consumers suffering from cheilitis, and has a broad market prospect.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and in particular relates to a moisturizing and anti-wrinkle composition and application thereof on lips. Background Art

[0002] The thickness of the human lip skin is much thinner than that of other parts of the body. Since the lips do not have melanocytes, they cannot effectively secrete melanin to fight against photoaging. At the same time, there are no sebaceous glands on the lips, which cannot spontaneously secrete sebum to form a protective layer of the sebum membrane. Therefore, the moisture loss rate of the lip skin is much faster than that of the facial skin. With the improvement of living standards and consumption upgrades, more and more women have higher requirements for lip makeup products.

[0003] There are many types of lip care products on the market, including lipsticks, lip balms, lipsticks, etc. Although these products are designed to improve or beautify the appearance of lips, they may also have some problems, which may involve the quality of the product itself, the effect of use, or the potential impact on the health of users. The following are some common problems: (1) Ingredient problems: Contains irritating ingredients: Some lip products may contain fragrances, preservatives or other chemicals, which may cause skin sensitivity or allergic reactions. (2) Harmful chemicals: Some products may contain chemicals that are harmful to the human body, such as lead, paraffin, etc. (3) Poor moisturizing effect: Although some lip balms can temporarily relieve dryness, long-term use may make the lips drier. (4) For patients with cheilitis, if the lip products are not suitable, they may aggravate the symptoms of cheilitis or cause new lip problems. (5) The ingredients of lip products are strictly regulated because the skin of the lips is thin and easily taken into the body. This means that some effective anti-wrinkle ingredients may not be used in lip care products. Some active ingredients may need to reach a certain concentration to exert an anti-wrinkle effect, but for safety reasons, the concentration of these ingredients is often limited in lip products, resulting in unsatisfactory anti-wrinkle effects of lip products.

[0004] In the prior art, dipalmitoyl hydroxyproline is used in lip products to make the lip products have a rapid anti-aging effect and excellent moisturizing effect. However, as a high-grade active ingredient, dipalmitoyl hydroxyproline may be relatively expensive, which may lead to high prices for lip products containing this ingredient. Although dipalmitoyl hydroxyproline is designed to be a skin-friendly ingredient, some people may still have sensitive or allergic reactions to it, especially for individuals with sensitive skin or existing cheilitis.

[0005] Therefore, there is an urgent need to develop a new and upgraded lip cosmetic composition to meet the upgrading needs of more and more women. Summary of the invention

[0006] The purpose of the present invention is to provide a moisturizing and anti-wrinkle composition and its application on lips.

[0007] In order to achieve the above object, the present invention provides the following technical solutions:

[0008] A moisturizing and anti-wrinkle composition, comprising the following components in parts by mass: 5-9 parts of composition A, 12-15 parts of composition B, 1-4 parts of bis-diglycerol polyacyl adipate-2, 1-3 parts of polyglyceryl-2 triisostearate, 0.1-8 parts of meadowfoam seed oil and 0.1-10 parts of argania spinosa kernel oil;

[0009] Composition A includes the following components in percentage by mass: 70-77% ethylhexyl palmitate, 18-23% tribehenin, 3-7% sorbitan isostearate, 0.1-0.5% palmitoyl tripeptide-1, and 0.1-3% lactic acid;

[0010] The composition B comprises the following components in percentage by mass: 84.5-97.5% of rice germ oil, 1-5% of rice bran oil, 0.1-1% of tocopherol acetate, and 1-10% of Bacillus fermentation product.

[0011] Furthermore, the preparation method of the Bacillus fermentation product is:

[0012] Step S1, culturing Bacillus in a culture medium to obtain Bacillus spores; the Bacillus includes Bacillus subtilis, Bacillus coagulans, and Bacillus megaterium;

[0013] Step S2, treating the Bacillus spores obtained in step S1 with lysozyme and protease and then with an ultra-high pressure homogenizer to obtain spore lysis products;

[0014] Step S3, centrifuging, concentrating and heating the spore lysis products obtained in step S2 to obtain Bacillus fermentation products.

[0015] Furthermore, the mass ratio of Bacillus subtilis, Bacillus coagulans, and Bacillus megaterium is 1:(1.3-1.6):(0.5-0.8).

[0016] Furthermore, the specific steps of culturing Bacillus in the culture medium in step S1 to obtain Bacillus spores are:

[0017] (1) Inoculate the Bacillus species into the seed culture medium and culture it aerobically at 30-35°C for 12-15 hours to obtain the seed solution;

[0018] (2) transplanting the seed solution into the fermentation medium and culturing it aerobically at 30-35°C for 40-45 hours to obtain the fermentation liquid;

[0019] (3) The fermentation broth is centrifuged and the precipitate is collected as Bacillus spores.

[0020] Furthermore, the specific steps of treating the Bacillus spores with lysozyme and protease and then with an ultra-high pressure homogenizer to obtain spore lysis products are as follows:

[0021] (1) Add pure water to the Bacillus spores to make the mass fraction of the Bacillus spores 3-6%, add lysozyme, and treat at a temperature of 50-53°C for 8-10 hours;

[0022] (2) Continue to add complex enzyme and treat at a temperature of 45-47°C for 5-7 hours to obtain a feed solution;

[0023] (3) The feed liquid is treated with an ultra-high pressure homogenizer to obtain a spore lysis product.

[0024] Furthermore, the composite enzyme is neutral protease, bromelain and ficin in a mass ratio of 1: (1.2-1.5): (0.6-0.8).

[0025] Furthermore, the added amount of the lysozyme is 0.1-0.3% of the mass of the Bacillus spores; the added amount of the complex enzyme is 0.1-0.3% of the mass of the Bacillus spores.

[0026] By adding composition A and composition B to the moisturizing and anti-wrinkle composition of the present invention, the moisturizing effect of the moisturizing and anti-wrinkle composition can be improved through the synergistic effect of the two compositions. Both composition A and composition B contain components with moisturizing effect, and by compounding multiple components, the moisturizing and anti-wrinkle composition can form a water-locking film on the lips to further improve the moisturizing effect. However, it was found in the experiment that the addition of the above components has an unsatisfactory anti-inflammatory effect on users suffering from cheilitis. The present invention adds a bacillus fermentation product prepared by a specific type of bacillus, and the dryness, keratinization, peeling, redness and swelling of the lips of users with cheilitis are significantly improved. The present invention ferments by using three specific ratios of bacillus, so that the bacillus fermentation product contains rich antioxidant and anti-inflammatory components, and multiple biologically active components can promote better absorption of other components, thereby improving the use effect of users with cheilitis. At the same time, the anti-wrinkle effect of the moisturizing and anti-wrinkle composition is improved.

[0027] Furthermore, the mass percentage of ethylhexyl palmitate in composition A is 72-75%, and the mass percentage of rice germ oil in composition B is 90-93%, and the mass percentage of Bacillus fermentation product is 4-6%.

[0028] The above moisturizing and anti-wrinkle composition has unsatisfactory stability after being prepared into lipstick. The present invention can improve the stability of the moisturizing and anti-wrinkle composition when added to lip products by limiting the usage amount of ethylhexyl palmitate, rice germ oil and Bacillus fermentation product. The reason is that ethylhexyl palmitate can be used as an emulsifier to help the oil and water phases to better combine and reduce the stratification phenomenon. The stable structure of fatty acids in rice germ oil can reduce the possibility of oxidation; the macromolecular substances in the Bacillus fermentation product, such as polysaccharides, can play a stabilizing role in the product and help maintain the stability of the product structure.

[0029] Furthermore, the preparation method of the moisturizing and anti-wrinkle composition comprises: weighing raw materials, stirring them uniformly at a rotation speed of 10-20 r / min, and sterilizing to obtain the moisturizing and anti-wrinkle composition.

[0030] The present invention also provides application of the moisturizing and anti-wrinkle composition in preparing lip products.

[0031] Compared with the prior art, the advantages and beneficial effects of the present invention are:

[0032] 1. The present invention provides an application of a moisturizing and anti-wrinkle composition in the preparation of lip products. The moisturizing and anti-wrinkle composition prepared by the present invention has a good moisturizing and anti-wrinkle effect, and is friendly to consumers suffering from cheilitis, and has broad market prospects.

[0033] 2. By adding composition A and composition B to the moisturizing and anti-wrinkle composition of the present invention, the moisturizing effect of the moisturizing and anti-wrinkle composition can be improved through the synergistic effect of the two compositions.

[0034] 3. The present invention uses 3 kinds of bacillus in specific ratios for fermentation, so that the fermented product of bacillus contains rich antioxidant and anti-inflammatory components, thereby improving the use effect of users with cheilitis and improving the anti-wrinkle effect of the moisturizing anti-wrinkle composition.

[0035] 4. The present invention can improve the stability of the moisturizing and anti-wrinkle composition when it is added to lip products by limiting the usage amounts of ethylhexyl palmitate, rice germ oil and Bacillus fermentation product. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] Figure 1 This is a picture of the cheilitis repair effect on a female subject. DETAILED DESCRIPTION

[0037] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0038] The raw materials used in the present invention are purchased from the following manufacturers:

[0039] Bacillus subtilis, accession number: SHBCCD53052, was purchased from Shanghai Microbiological Collection Center.

[0040] Bacillus coagulans, accession number: SHBCCD10638, was purchased from Shanghai Microbiological Collection Center.

[0041] Bacillus megaterium, accession number: SHBCCD73444, was purchased from Shanghai Microbiological Collection Center.

[0042] Neutral protease, enzyme activity 100u / mg, Shanghai Yuanye Biotechnology Co., Ltd.

[0043] Bromelain, enzyme activity 300u / mg, Shanghai Yuanye Biotechnology Co., Ltd.

[0044] Fig proteinase, enzyme activity 300u / mg, Shanghai Yuanye Biotechnology Co., Ltd.

[0045] The composition of the seed culture medium is: 10 g / L cellobiose, 12 g / L corn steep liquor, 2 g / L tripotassium citrate, 1.25 g / L citric acid monohydrate, 1 g / L sodium sulfate, 1 g / L potassium dihydrogen phosphate, 2.5 g / L sodium bicarbonate, 1.5 g / L ammonium chloride, 5 g / L urea, 1 g / L yeast extract, 50 g / L magnesium chloride hexahydrate, 0.1 g / L ferrous chloride tetrahydrate, 0.2 g / L calcium chloride dihydrate, 1 g / L cysteine ​​hydrochloride monohydrate, 0.02 g / L pyridoxamine dihydrochloride, 0.002 g / L D-biotin, and 0.02 g / L vitamin B12.

[0046] The fermentation medium includes the following components: 4 g / L cellobiose, 8 g / L corn steep liquor, 7 g / L peptone, 3 g / L soybean powder, 9 g / L glucose, 15 g / L glycerol, 5 g / L sodium glutamate, 4 g / L tripotassium citrate, 1.3 g / L potassium dihydrogen phosphate, 3 g / L sodium bicarbonate, 2 g / L ammonium chloride, 5 g / L urea, 1.2 g / L yeast extract, 0.7 g / L calcium chloride dihydrate, 1.5 g / L magnesium sulfate, and 0.007 g / L aminoadipic acid, and is prepared with water. Example 1

[0047] This embodiment provides a moisturizing and anti-wrinkle composition, comprising the following components in parts by mass: 7 parts of composition A, 14 parts of composition B, 3 parts of bis-diglyceryl polyacyl adipate-2, 2 parts of polyglyceryl-2 triisostearate, 5 parts of meadowfoam seed oil, and 9 parts of argania spinosa kernel oil;

[0048] Composition A includes the following components in percentage by mass: 74% ethylhexyl palmitate, 20.2% tribehenin, 5% sorbitan isostearate, 0.4% palmitoyl tripeptide-1, and 0.4% lactic acid.

[0049] Composition B includes the following components in percentage by mass: 92% rice germ oil, 3% rice bran oil, 0.5% tocopherol acetate, and 4.5% Bacillus fermentation product.

[0050] The preparation method of the bacillus fermentation product is:

[0051] Step S1, culturing Bacillus in a culture medium to obtain Bacillus spores; the Bacillus includes Bacillus subtilis, Bacillus coagulans, and Bacillus megaterium;

[0052] Step S2, treating the Bacillus spores obtained in step S1 with lysozyme and protease and then with an ultra-high pressure homogenizer to obtain spore lysis products;

[0053] Step S3, centrifuging, concentrating and heating the spore lysis products obtained in step S2 to obtain Bacillus fermentation products.

[0054] The specific steps of culturing Bacillus in the culture medium in step S1 to obtain Bacillus spores are:

[0055] (1) Bacillus subtilis, Bacillus coagulans, and Bacillus megaterium were mixed in a mass ratio of 1:1.5:0.7 (the mass ratio refers to the mass ratio of the lyophilized materials of Bacillus subtilis, Bacillus coagulans, and Bacillus megaterium, purchased from Shanghai Collection of Microorganisms Center, the same below) to obtain Bacillus strains, inoculated the Bacillus strains into a seed culture medium, and cultured aerobically at 32°C for 13 h to obtain a seed solution;

[0056] (2) The seed solution was transplanted into the fermentation medium and cultured aerobically at 32°C for 42 hours to obtain the fermentation liquid;

[0057] (3) The fermentation broth is centrifuged and the precipitate is collected as Bacillus spores.

[0058] The specific steps of treating the Bacillus spores with lysozyme and protease and then with an ultra-high pressure homogenizer to obtain spore lysis products are as follows:

[0059] (1) Add pure water to the Bacillus spores to make the mass fraction of the Bacillus spores 5%, add lysozyme, and treat at a temperature of 52°C for 9 hours;

[0060] (2) Continue to add complex enzyme and treat at 46°C for 6 hours to obtain liquid;

[0061] (3) The feed liquid is treated with an ultra-high pressure homogenizer to obtain a spore lysis product.

[0062] The complex enzyme is neutral protease, bromelain and ficin in a mass ratio of 1:1.4:0.7.

[0063] The amount of lysozyme added is 0.2% of the mass of Bacillus spores; the amount of complex enzyme added is 0.2% of the mass of Bacillus spores;

[0064] The preparation method of the moisturizing and anti-wrinkle composition comprises: weighing raw materials, stirring them uniformly under a stirring condition of a rotation speed of 15 r / min, and sterilizing to obtain the moisturizing and anti-wrinkle composition. Example 2

[0065] This embodiment provides a moisturizing and anti-wrinkle composition, comprising the following components in parts by mass: 5 parts of composition A, 12 parts of composition B, 1 part of bis-diglyceryl polyacyl adipate-2, 1 part of polyglyceryl-2 triisostearate, 3 parts of meadowfoam seed oil, and 7 parts of argania spinosa kernel oil;

[0066] Composition A includes the following components in percentage by mass: 72% ethylhexyl palmitate, 21.2% tribehenin, 6% sorbitan isostearate, 0.4% palmitoyl tripeptide-1, and 0.4% lactic acid.

[0067] Composition B includes the following components in percentage by mass: 90% rice germ oil, 4% rice bran oil, 0.5% tocopherol acetate, and 5.5% Bacillus fermentation product.

[0068] The preparation method of the bacillus fermentation product is:

[0069] Step S1, culturing Bacillus in a culture medium to obtain Bacillus spores; the Bacillus includes Bacillus subtilis, Bacillus coagulans, and Bacillus megaterium;

[0070] Step S2, treating the Bacillus spores obtained in step S1 with lysozyme and protease and then with an ultra-high pressure homogenizer to obtain spore lysis products;

[0071] Step S3, centrifuging, concentrating and heating the spore lysis products obtained in step S2 to obtain Bacillus fermentation products.

[0072] The specific steps of culturing Bacillus in the culture medium in step S1 to obtain Bacillus spores are:

[0073] (1) Bacillus subtilis, Bacillus coagulans, and Bacillus megaterium were mixed in a mass ratio of 1:1.3:0.5 to obtain Bacillus strains, the Bacillus strains were inoculated into a seed culture medium, and aerobically cultured at a temperature of 30°C for 12 hours to obtain a seed solution;

[0074] (2) The seed solution was transplanted into the fermentation medium and cultured aerobically at 30°C for 40 hours to obtain the fermentation liquid;

[0075] (3) The fermentation broth is centrifuged and the precipitate is collected as Bacillus spores.

[0076] The specific steps of treating the Bacillus spores with lysozyme and protease and then with an ultra-high pressure homogenizer to obtain spore lysis products are as follows:

[0077] (1) adding pure water to the Bacillus spores to make the mass fraction of the Bacillus spores 3%, adding lysozyme, and treating at a temperature of 50°C for 8 hours;

[0078] (2) Continue to add complex enzyme and treat at 45°C for 5 hours to obtain liquid;

[0079] (3) The feed liquid is treated with an ultra-high pressure homogenizer to obtain a spore lysis product.

[0080] The complex enzyme is neutral protease, bromelain and ficin in a mass ratio of 1:1.2:0.6.

[0081] The amount of lysozyme added is 0.1% of the mass of Bacillus spores; the amount of complex enzyme added is 0.1% of the mass of Bacillus spores;

[0082] The preparation method of the moisturizing and anti-wrinkle composition comprises: weighing raw materials, stirring them evenly under a stirring condition of a rotation speed of 10 r / min, and sterilizing to obtain the moisturizing and anti-wrinkle composition. Example 3

[0083] This embodiment provides a moisturizing and anti-wrinkle composition, comprising the following components in parts by mass: 9 parts of composition A, 15 parts of composition B, 4 parts of bis-diglyceryl polyacyl adipate-2, 3 parts of polyglyceryl-2 triisostearate, 8 parts of meadowfoam seed oil and 10 parts of argania spinosa kernel oil;

[0084] Composition A includes the following components in percentage by mass: 75% ethylhexyl palmitate, 19.2% tribehenin, 5% sorbitan isostearate, 0.4% palmitoyl tripeptide-1, and 0.4% lactic acid.

[0085] Composition B includes the following components in percentage by mass: 93% rice germ oil, 2% rice bran oil, 0.5% tocopherol acetate, and 4.5% Bacillus fermentation product.

[0086] The preparation method of the bacillus fermentation product is:

[0087] Step S1, culturing Bacillus in a culture medium to obtain Bacillus spores; the Bacillus includes Bacillus subtilis, Bacillus coagulans, and Bacillus megaterium;

[0088] Step S2, treating the Bacillus spores obtained in step S1 with lysozyme and protease and then with an ultra-high pressure homogenizer to obtain spore lysis products;

[0089] Step S3, centrifuging, concentrating and heating the spore lysis products obtained in step S2 to obtain Bacillus fermentation products.

[0090] The specific steps of culturing Bacillus in the culture medium in step S1 to obtain Bacillus spores are:

[0091] (1) Bacillus subtilis, Bacillus coagulans, and Bacillus megaterium were mixed in a mass ratio of 1:1.6:0.8 to obtain Bacillus strains, the Bacillus strains were inoculated into a seed culture medium, and aerobically cultured at a temperature of 35°C for 15 hours to obtain a seed solution;

[0092] (2) The seed solution was transplanted into the fermentation medium and cultured aerobically at 35°C for 45 hours to obtain the fermentation liquid;

[0093] (3) The fermentation broth is centrifuged and the precipitate is collected as Bacillus spores.

[0094] The specific steps of treating the Bacillus spores with lysozyme and protease and then with an ultra-high pressure homogenizer to obtain spore lysis products are as follows:

[0095] (1) Add pure water to the Bacillus spores to make the mass fraction of the Bacillus spores 6%, add lysozyme, and treat at 53°C for 10 hours;

[0096] (2) Continue to add complex enzyme and treat at 47°C for 7 hours to obtain liquid feed;

[0097] (3) The feed liquid is treated with an ultra-high pressure homogenizer to obtain a spore lysis product.

[0098] The complex enzyme is neutral protease, bromelain and ficin in a mass ratio of 1:1.5:0.8.

[0099] The amount of lysozyme added is 0.3% of the mass of Bacillus spores; the amount of complex enzyme added is 0.3% of the mass of Bacillus spores;

[0100] The preparation method of the moisturizing and anti-wrinkle composition comprises: weighing raw materials, stirring them uniformly under a stirring condition of a rotation speed of 20 r / min, and sterilizing to obtain the moisturizing and anti-wrinkle composition.

[0101] Comparative Example 1

[0102] The difference between this comparative example and Example 1 is: a moisturizing and anti-wrinkle composition, comprising the following components in parts by mass: 14 parts of composition A, 7 parts of composition B, 3 parts of bis-diglyceryl polyacyl adipate-2, 2 parts of polyglyceryl-2 triisostearate, 5 parts of white meadowfoam seed oil and 9 parts of argania spinosa kernel oil.

[0103] Comparative Example 2

[0104] The difference between this comparative example and Example 1 is that composition A includes the following components in mass percentage: 69% ethylhexyl palmitate, 25.2% tribehenin, 5% sorbitan isostearate, 0.4% palmitoyl tripeptide-1, and 0.4% lactic acid.

[0105] Comparative Example 3

[0106] The difference between this comparative example and Example 1 is that composition B includes the following components in mass percentages: 87% rice germ oil, 3% rice bran oil, 0.5% tocopherol acetate, and 9.5% Bacillus fermentation product.

[0107] Comparative Example 4

[0108] The difference between this comparative example and Example 1 is that the mass ratio of Bacillus subtilis, Bacillus coagulans and Bacillus megaterium is 1:1:1.

[0109] Comparative Example 5

[0110] The difference between this comparative example and Example 1 is that the Bacillus fermentation product is a commercially available product purchased from Hubei Xinyuhong Biopharmaceutical Technology Co., Ltd.

[0111] Comparative Example 6

[0112] The difference between this comparative example and Example 1 is that the composite enzyme is neutral protease, bromelain and ficin in a mass ratio of 1:1:1.

[0113] Performance Testing

[0114] 55 parts by mass of solid wax base (mass ratio: 20% cocoa butter, 12% jasmine wax, 14% beeswax, balance avocado oil) were heated and melted until completely liquid, and 45 parts by mass of the composition prepared in Examples 1-3 and Comparative Examples 1-6 were added under low-speed stirring; the speed was 20 r / min, the mixture was mixed and stirred for 40 minutes, and the lipstick was discharged and filled. The lipstick was tested for performance:

[0115] 1. The physical and chemical properties of lipstick were measured according to standard QB / T1977-2004. The results are shown in Table 1.

[0116] Table 1 Performance test results

[0117] Sensory indicators cold resistance Heat resistant Example 1 good good good Example 2 good good good Example 3 good good good Comparative Example 1 good There are cracks There are cracks Comparative Example 2 good good There are cracks Comparative Example 3 good good There are cracks Comparative Example 4 good good good Comparative Example 5 good good There are cracks Comparative Example 6 good good good

[0118] The results show that the sensory indicators of the lipsticks obtained in Examples 1-3 are all good, with a smooth appearance without bubbles, no obvious impurities, uniform color of the paste, and no stratification. The cold and heat resistance is good. After being placed at -10°C (cold resistance) and 45°C (heat resistance) for 24 hours, it is taken out and restored to room temperature without softening or cracking, and its use effect and state have no obvious adverse effects.

[0119] 2. Anti-wrinkle effect test

[0120] The lipsticks prepared in Examples 1-3 and Comparative Examples 1-6 were tested on 30 subjects for each lipstick. The subjects were healthy women (i.e., non-allergic) aged between 20 and 60 years old, and the main symptoms were wrinkles, chapped skin and inflammation on the lips.

[0121] Usage: The subjects should apply lipstick to the test area 15 minutes after cleansing every day, and apply it once after breakfast, lunch, dinner and before going to bed.

[0122] Specific testing method: The inner side of the arm (specifically the middle of the forearm) was used instead of the lips for testing. The subjects applied lipstick on the inner side of their arms after daily cleaning. After 2 months of use, the DermaLab Combo-skin tester (Cortex Technology Company, Denmark) was used to perform skin ultrasound testing on the inner side of the arms of the subjects. The test results of the subjects were recorded and shown in Table 2. Among them, LEB represents the gap between the epidermis and the dermis, in μm; the larger the gap, the more serious the aging.

[0123] The calculation formula of the LEB value improvement rate is: {(LEB value of the inner side of the subject's arm before using the lipstick-LEB value of the inner side of the subject's arm 2 months after using the lipstick) / LEB value of the inner side of the subject's arm before using the lipstick}×100%.

[0124] Table 2 Test results

[0125] LEB value improvement rate % Example 1 25.6 Example 2 25.0 Example 3 25.2 Comparative Example 1 20.5 Comparative Example 2 22.5 Comparative Example 3 21.7 Comparative Example 4 21.3 Comparative Example 5 20.4 Comparative Example 6 22.4

[0126] 3. Moisturizing effect test

[0127] The test subjects are the same as those for the anti-wrinkle effect test, the only difference is the test method, which is as follows:

[0128] Specific detection method: Considering that applying lipstick will have a significant impact on the TEWL test due to factors such as exhalation, the inner side of the arm (specifically the middle of the forearm) is used instead of the lips for testing. The subjects clean the inner side of their arms and apply lipstick. 6 hours after application, the TEWL of the inner side of the subjects' arms is tested using the DermaLabCombo-skin detector (Cortex Technology Company, Denmark). The test results of the subjects are recorded, and the TEWL of the inner side of the subjects' arms is analyzed and recorded based on the test results collected each time. The results are shown in Table 3.

[0129] The calculation formula for the improvement rate of TEWL rate is: {(TEWL rate of the inner side of the subject's arm before using the lipstick - TEWL rate of the inner side of the same subject's arm 6 hours after using the lipstick) / TEWL rate of the inner side of the subject's arm before using the lipstick}×100%.

[0130] Table 3 Performance test results

[0131] Transepidermal water loss rate improvement rate % Example 1 65.46 Example 2 64.78 Example 3 65.02 Comparative Example 1 51.25 Comparative Example 2 53.97 Comparative Example 3 54.65 Comparative Example 4 52.75 Comparative Example 5 48.35 Comparative Example 6 57.59

[0132] 4. Test sample: lipstick sample made from the composition of Example 1.

[0133] Subjects: 20 females aged 25 years, with symptoms of cheilitis such as dry lips, keratinization, peeling, redness and swelling.

[0134] Test method: Clean and dry the test subject's lips once in the morning and evening every day for 15 consecutive days. The symptoms of cheilitis such as dryness, keratinization, peeling, redness and swelling of the test subject's lips are effectively alleviated.

[0135] Figure 1 The following are comparative pictures of a tester using the lipstick prepared in Example 1 of the present invention before application and 15 days after application (photographed on the 16th day).

[0136] pass Figure 1 It can be seen that the dryness, keratinization, peeling, redness and swelling of the lips of the subjects were significantly improved. It can also play an anti-inflammatory role on the dry, chapped, and reddish skin around the lips of the subjects.

[0137] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. Use of a Bacillus fermentation product in preparing a moisturizing and anti-wrinkle composition for improving dryness, keratinization, peeling, and redness of the lips of users with cheilitis, characterized in that: The moisturizing and anti-wrinkle composition is composed of the following components in parts by mass: 5-9 parts of composition A, 12-15 parts of composition B, 1-4 parts of bis-diglycerol polyacyl adipate-2, 1-3 parts of polyglyceryl-2 triisostearate, 0.1-8 parts of meadowfoam seed oil and 0.1-10 parts of argania spinosa kernel oil; Composition A is composed of the following components in percentage by mass: 72-75% ethylhexyl palmitate, 18-23% tribehenin, 3-7% sorbitan isostearate, 0.1-0.5% palmitoyl tripeptide-1, and 0.1-3% lactic acid; Composition B is composed of the following components in percentage by mass: 90-93% rice germ oil, 1-5% rice bran oil, 0.1-1% tocopherol acetate, and 4-6% Bacillus fermentation product; the preparation method of the Bacillus fermentation product is as follows: Step S1, culturing Bacillus in a culture medium to obtain Bacillus spores; the Bacillus includes Bacillus subtilis, Bacillus coagulans, and Bacillus megaterium; Step S2, treating the Bacillus spores obtained in step S1 with lysozyme and complex enzyme and then with an ultra-high pressure homogenizer to obtain spore lysis products; Step S3, centrifuging, concentrating and heating the spore lysis products obtained in step S2 to obtain a Bacillus fermentation product; The mass ratio of Bacillus subtilis, Bacillus coagulans, and Bacillus megaterium is 1:(1.3-1.6):(0.5-0.8); The specific steps of culturing Bacillus in the culture medium in step S1 to obtain Bacillus spores are: (1) Inoculate the Bacillus species into the seed culture medium and culture it aerobically at 30-35°C for 12-15 hours to obtain the seed solution; (2) transplanting the seed solution into the fermentation medium and culturing it aerobically at 30-35°C for 40-45 hours to obtain the fermentation liquid; (3) centrifuging the fermentation broth and collecting the precipitate as Bacillus spores; In step S2, the Bacillus spores are treated with lysozyme and complex enzyme and then treated with an ultra-high pressure homogenizer to obtain spore lysis products in the following specific steps: (1) Add pure water to the Bacillus spores to make the mass fraction of the Bacillus spores 3-6%, add lysozyme, and treat at a temperature of 50-53°C for 8-10 hours; (2) Continue to add complex enzyme and treat at a temperature of 45-47°C for 5-7 hours to obtain a feed solution; (3) treating the feed liquid with an ultra-high pressure homogenizer to obtain a spore lysis product; The composite enzyme is neutral protease, bromelain and ficin in a mass ratio of 1: (1.2-1.5): (0.6-0.8); The added amount of the lysozyme is 0.1-0.3% of the mass of the bacillus spores; the added amount of the complex enzyme is 0.1-0.3% of the mass of the bacillus spores.

2. The use according to claim 1, characterized in that The preparation method of the moisturizing and anti-wrinkle composition comprises: weighing raw materials, and stirring them evenly under a stirring condition of a rotation speed of 10-20 r / min to obtain the moisturizing and anti-wrinkle composition.

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