A composition for resisting glycation and delaying aging and preparation method thereof

Abstract

The invention discloses an anti-glycation and anti-aging composition and a preparation method thereof, and relates to the technical field of functional foods. The raw materials of the composition of the invention are composed of the following components by weight: 1-10 parts of ginger powder, 1-10 parts of mulberry powder, 0.1-10 parts of sea buckthorn powder, 1-20 parts of light bamboo leaf powder, 1-15 parts of grape seed extract, 1-10 parts of Agaricus bisporus extract and 1-13 parts of Haematococcus pluvialis astaxanthin microcapsule powder. The invention simplifies the formula ingredients, improves safety, does not produce side effects, has a simple preparation process, and has good anti-glycation and anti-aging effects.

Description

Technical Field

[0001] The invention relates to the technical field of functional foods, in particular to an anti-glycation and anti-aging composition and a preparation method thereof. Background Art

[0002] Skin aging is an irresistible natural law for humans, and skin glycation will accelerate skin aging. Glycation mainly refers to the effect of sugar on collagen and elastin in the dermis of the skin - glycated collagen and elastin will lose their physiological functions and support and elasticity for skin tissue, causing the skin to show signs of aging. The main manifestations are yellowish and dull skin color, acne, enlarged pores, and loss of skin elasticity. Therefore, anti-glycation is to prevent the skin's collagen and elastin from losing vitality, so that the skin can reach a younger state.

[0003] In addition to endogenous factors such as the polyol pathway, there are also some exogenous factors that accelerate the glycation process of the skin, such as tobacco, ultraviolet rays, air pollution, etc. The glycation process is accompanied by the generation of free radicals (ROS), and advanced glycation end products (AGEs) are one of the important factors that accelerate skin glycation.

[0004] Antioxidant is the abbreviation of antioxidant free radicals. The human body continuously produces free radicals in the body due to continuous contact with the outside world, including breathing (oxidation reaction), external pollution, radiation exposure and other factors. Cancer, aging or some other diseases are related to the production of excessive free radicals. And more and more studies have shown that antioxidation is an important step in preventing aging. Free radicals or oxidants will decompose cells and tissues, affect metabolic functions, and cause different health problems. If excessive oxidative free radicals can be eliminated, many aging-related diseases caused by free radicals can be prevented.

[0005] Ginger powder can accelerate blood flow, improve peripheral circulation, inhibit the formation of AGEs, thereby blocking the cross-linking between proteins and reducing the retention and deposition of AGEs in the skin microcirculation process. Mulberry powder can denature the protein structure, so that it cannot combine with the intermediate carbonyl compound, protect the free amino group, and block the glycosylation reaction. The polyphenols such as anthocyanins in mulberry powder can reduce the content of malondialdehyde (MDA) in myocardial mitochondria, increase the amount of superoxide dismutase (SOD) in myocardial mitochondria, and improve the total antioxidant capacity. Mulberry powder also contains a high level of resveratrol, which can weaken the pro-inflammatory response and exert anti-inflammatory effects. The total flavonoids of seabuckthorn powder have the effect of inhibiting the formation of AGEs. It inhibits the formation of AGEs by inhibiting the expression level of the receptor for advanced glycation end products mRNA. The flavonoid extract in seabuckthorn powder has a strong scavenging ability for hydroxyl free radicals, and can also reduce the formation of lipid peroxidation product MDA, inhibit cell apoptosis and thus delay aging. In addition, the flavonoids of seabuckthorn powder also have the effect of inhibiting tyrosinase activity and whitening. Bamboo leaf flavonoids can inhibit the rearrangement of early glycosylation products and oxidize them to form carbonyl compounds to block non-enzymatic glycosylation reactions. It can also increase the activity of antioxidant enzymes, inhibit lipid peroxidation products, protect collagen, and delay aging.

[0006] Chinese patent CN110574927A discloses an anti-glycation composition and its preparation method, which is specifically as follows by weight: 1-30% apple, 1-25% red pomegranate, 0.5-20% light bamboo leaf powder, 0.5-20% bonito oligopeptide, 0.5-20% grape, 0.5-10% black currant, 0.1-10% mulberry powder, 0.1-10% kudzu root, 0.1-10% sea buckthorn powder, 0.1-10% black ginger powder, 0.01-5% epigallocatechin gallate, and the balance of auxiliary materials. The composition can be made into tablets, granules, capsules, soft capsules, powders or oral liquids. The present invention combines functional compounds, functional foods and traditional Chinese medicines, starting from the human body's metabolic mechanism, and comprehensively resisting glycation, while taking into account the effects of anti-oxidation, anti-aging, anti-inflammatory and whitening. This patented formula has many ingredients, and basically all ingredients must be prepared through a multi-step extraction process. Bonito oligopeptide powder requires the use of multiple enzymes for complex enzymatic hydrolysis, which makes the preparation complex and costly.

[0007] At present, there are few products on the market that can both resist glycation and delay aging, and they generally have the disadvantages of slow effect and high cost, and products with significant efficacy and high safety are relatively rare. In view of this, the present invention provides a composition for resisting glycation and delaying aging and a preparation method thereof. Summary of the invention

[0008] The purpose of the present invention is to provide an anti-glycation and anti-aging composition and a preparation method thereof, which simplifies the formula ingredients, improves safety, does not produce side effects, has a simple preparation process, and has good anti-glycation and anti-aging effects, and is suitable for people who need anti-glycation and anti-aging effects.

[0009] To achieve the above-mentioned purpose of the invention, the technical solution of the present invention is as follows:

[0010] In one aspect, the present invention provides an anti-glycation and anti-aging composition, which comprises the following ingredients by weight: 1-10 parts of ginger powder, 1-10 parts of mulberry powder, 0.1-10 parts of sea buckthorn powder, 1-20 parts of bamboo leaf powder, 1-15 parts of grape seed extract, 1-10 parts of Agaricus bisporus extract and 1-13 parts of Haematococcus pluvialis astaxanthin microcapsule powder;

[0011] The grape seed extract is prepared according to the following method:

[0012] S1, extracting grape seeds by heating and refluxing with a solvent, and drying the extract to obtain a crude grape seed extract;

[0013] S2, applying the crude grape seed extract obtained in step S1 to a macroporous resin column, and eluting with ethanol aqueous solution with a volume fraction of 20-30% and 50-60% in sequence, collecting the eluate eluted with the volume fraction of 50-60% ethanol aqueous solution, and drying to obtain a grape seed eluate;

[0014] S3. The grape seed eluate obtained in step S2 is placed on a silica gel column, and gradient eluted with an organic solvent consisting of ethyl acetate and methanol in a volume ratio of 85-95:5-15 and 80-85:15-20, respectively, collecting the eluate eluted with the organic solvent consisting of ethyl acetate and methanol in a volume ratio of 80-85:15-20, and drying to obtain a grape seed extract.

[0015] Preferably, the anti-glycation and anti-aging composition consists of the following ingredients in parts by weight: 1-3 parts of ginger powder, 2-8 parts of mulberry powder, 0.5-5 parts of sea buckthorn powder, 2-8 parts of loblolly leaf powder, 2-10 parts of grape seed extract, 2-8 parts of Agaricus bisporus extract and 2-8 parts of astaxanthin microcapsule powder from Haematococcus pluvialis.

[0016] Further preferably, the anti-glycation and anti-aging composition consists of the following ingredients in parts by weight: 1-2 parts of ginger powder, 4-6 parts of mulberry powder, 1-2 parts of sea buckthorn powder, 4-5 parts of loblolly leaf powder, 3-6 parts of grape seed extract, 3-4 parts of Agaricus bisporus extract and 5-6 parts of Haematococcus pluvialis astaxanthin microcapsule powder.

[0017] Most preferably, the anti-glycation and anti-aging composition consists of the following ingredients in parts by weight: 1.5 parts of ginger powder, 4 parts of mulberry powder, 2 parts of sea buckthorn powder, 5 parts of loblolly leaf powder, 4 parts of grape seed extract, 4 parts of Agaricus bisporus extract and 5 parts of astaxanthin microcapsule powder from Haematococcus pluvialis.

[0018] On the other hand, the method for preparing the anti-glycation and anti-aging composition of the present invention comprises the steps of:

[0019] (1) Mix ginger powder, mulberry powder, sea buckthorn powder, and bamboo leaf powder in the prescribed amount, and sieve to obtain a mixture;

[0020] (2) mixing the mixture obtained in step (1) with a solvent, extracting, combining the extracts, concentrating, and drying to obtain an extract;

[0021] (3) The extract obtained in step (2) is mixed with a formula amount of grape seed extract, Agaricus bisporus extract, and Haematococcus pluvialis astaxanthin microcapsule powder to obtain the anti-glycation and anti-aging composition.

[0022] Preferably, in step (1), the sieving is through a 60-mesh sieve.

[0023] Preferably, in step (2), the solvent is water or ethanol aqueous solution; further preferably, the volume concentration of the ethanol aqueous solution is 10-50%.

[0024] Preferably, in step (2), the extraction conditions are: solid-liquid ratio of 1:8-15, extraction time of 1-4h, and extraction times of 2-4 times; further preferably, the extraction conditions are: solid-liquid ratio of 1:10-12, extraction time of 2-3h, and extraction times of 2-3 times; most preferably, the extraction conditions are: solid-liquid ratio of 1:10 or 1:12, extraction time of 2h, and extraction times of 3 times.

[0025] The term "material-liquid ratio" refers to the ratio of the mass of the raw material to be extracted to the volume of the extraction solvent, with the unit of g:mL.

[0026] Preferably, the grape seed extract is prepared according to the following method:

[0027] S1, extracting grape seeds by heating and refluxing with a solvent, and drying the extract to obtain a crude grape seed extract;

[0028] S2, applying the crude grape seed extract obtained in step S1 to a macroporous resin column, and eluting with ethanol aqueous solution with a volume fraction of 20-30% and 50-60% in sequence, collecting the eluate eluted with the volume fraction of 50-60% ethanol aqueous solution, and drying to obtain a grape seed eluate;

[0029] S3. The grape seed eluate obtained in step S2 is placed on a silica gel column, and gradient eluted with an organic solvent consisting of ethyl acetate and methanol in a volume ratio of 85-95:5-15 and 80-85:15-20, respectively, collecting the eluate eluted with the organic solvent consisting of ethyl acetate and methanol in a volume ratio of 80-85:15-20, and drying to obtain a grape seed extract.

[0030] Preferably, in step S1, the solvent is at least one of water and ethanol aqueous solution; more preferably, it is ethanol aqueous solution, further preferably, it is ethanol aqueous solution with a volume concentration of 40-60%, and most preferably, it is ethanol aqueous solution with a volume concentration of 50%.

[0031] Preferably, in step S1, the conditions for the heating reflux extraction are: a solid-liquid ratio of 1:10-20, and an extraction time of 1.5-3 hours; most preferably, the conditions for the heating reflux extraction are: a solid-liquid ratio of 1:15, and an extraction time of 2 hours.

[0032] Preferably, in step S2, the macroporous resin column is a H103 macroporous resin column.

[0033] Preferably, in step S2, gradient elution is performed with ethanol aqueous solutions with volume fractions of 25% and 55% in sequence, and the eluate eluted with the ethanol aqueous solution with a volume fraction of 55% is collected.

[0034] Preferably, in step S3, the silica gel column is FCP, 100-140 mesh silica gel.

[0035] Preferably, in step S3, gradient elution is performed with an organic solvent consisting of ethyl acetate and methanol in a volume ratio of 89:11 and 83:17, respectively, and the eluate eluted with the organic solvent consisting of ethyl acetate and methanol in a volume ratio of 83:17 is collected.

[0036] Preferably, in step S3, the water content of the grape seed extract does not exceed 5%.

[0037] The beneficial effects of the present invention are:

[0038] (1) The present invention simplifies the ingredients of the formula, improves safety, does not produce side effects, has a simple preparation process, and has good anti-glycation and anti-aging effects.

[0039] (2) The present invention obtains a formula having good antioxidant and anti-glycation effects through specific compatibility and ratio screening. In the formula, the Agaricus bisporus extract has a synergistic effect with other ingredients.

[0040] (3) The present invention optimizes the extraction method of grape seeds. After the grape seed extract is combined with other ingredients to form a formula, the antioxidant and anti-glycation effects of the entire formula are significantly improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0041] Figure 1 This is a process flow chart of Example 1 of the present invention. DETAILED DESCRIPTION

[0042] The following non-limiting examples can enable those skilled in the art to more fully understand the present invention, but do not limit the present invention in any way. The following content is only an exemplary description of the scope of the present invention, and those skilled in the art can make various changes and modifications to the invention of the present invention based on the disclosed content, and they should also fall within the scope of the present application.

[0043] The present invention is further described below by way of specific examples. The various chemical reagents used in the examples of the present invention are obtained through conventional commercial channels unless otherwise specified. Unless otherwise specified, the contents described below are all weight contents. Unless otherwise specified, it is understood that the process is carried out at room temperature.

[0044] Some of the raw materials in the examples are from the following sources:

[0045]

[0046] Examples 1-6

[0047] The formulas of Examples 1-6 are as follows:

[0048]

[0049] The grape seed extracts in Examples 1-6 were all prepared by the following method:

[0050] S1, heating and refluxing the grape seeds with an ethanol aqueous solution having a volume concentration of 50%, the conditions for the heating and refluxing extraction being: a solid-liquid ratio of 1:15, an extraction time of 2 hours, and drying the extract to obtain a crude grape seed extract;

[0051] S2, applying the crude grape seed extract obtained in step S1 to a H103 macroporous resin column, and eluting with 25% (elution volume 4BV, flow rate 2BV / h) and 55% ethanol aqueous solution (elution volume 10BV, flow rate 2BV / h) in a gradient manner, collecting the eluate eluted with 55% ethanol aqueous solution, and drying to obtain a grape seed eluate;

[0052] S3. The grape seed eluate obtained in step S2 was loaded onto a silica gel column (FCP, 120 mesh), and gradient eluted with an organic solvent consisting of ethyl acetate and methanol in a volume ratio of 89:11 and 83:17 (the elution volume was 6 BV and the flow rate was 2 BV / h), and the eluate eluted with the organic solvent consisting of ethyl acetate and methanol in a volume ratio of 83:17 was collected and dried to obtain a grape seed extract with a moisture content of <5%.

[0053] Examples 1-6 all prepared the compositions according to the following steps:

[0054] (1) Mix ginger powder, mulberry powder, sea buckthorn powder and bamboo leaf powder in the prescribed amount, and pass through a 60-mesh sieve to obtain a mixture;

[0055] (2) mixing the mixture obtained in step (1) with an ethanol aqueous solution having a volume concentration of 30%, with a solid-liquid ratio of 1:10, the extraction time being 2 h, the extraction being performed 3 times, the extracts being combined, concentrated, and dried to obtain an extract;

[0056] (3) The extract obtained in step (2) is mixed with a formulated amount of grape seed extract, Agaricus bisporus extract, and Haematococcus pluvialis astaxanthin microcapsule powder to obtain an anti-glycation and anti-aging composition.

[0057] Example 7

[0058] The difference from Example 2 is that in the preparation process, in step (2), an ethanol aqueous solution with a volume concentration of 50% is used, and the rest is the same.

[0059] Example 8

[0060] The difference from Example 2 is that the grape seed extract is a commercially available product (purchased from Xi'an Tianyi Biotechnology Co., Ltd.), and the rest are the same.

[0061] Comparative Example 1-2

[0062] The formula of Comparative Example 1-2 is as follows, and the rest is the same as Example 2:

[0063]

[0064] Comparative Example 3

[0065] The difference from Example 2 is that the Agaricus bisporus extract is replaced with an equal amount of mulberry leaf extract (purchased from Hunan Hill Natural Pharmaceutical Co., Ltd.), and the rest is the same.

[0066] Comparative Example 4

[0067] The difference from Example 2 is that the grape seed extract is replaced by an equal amount of the grape seed eluate obtained in step S2, and the rest is the same.

[0068] Comparative Example 5

[0069] The difference from Example 2 is that the grape seed extract is prepared by the following method, and the rest is the same:

[0070] S1, heating and refluxing the grape seeds with an ethanol aqueous solution having a volume concentration of 50%, the conditions for the heating and refluxing extraction being: a solid-liquid ratio of 1:15, an extraction time of 2 hours, and drying the extract to obtain a crude grape seed extract;

[0071] S2, applying the crude grape seed extract obtained in step S1 to a H103 macroporous resin column, and eluting with 25% (elution volume 4BV, flow rate 2BV / h) and 55% ethanol aqueous solution (elution volume 10BV, flow rate 2BV / h) in a gradient manner, collecting the eluate eluted with 55% ethanol aqueous solution, and drying to obtain a grape seed eluate;

[0072] S3. The grape seed eluate obtained in step S2 was loaded onto a silica gel column (FCP, 120 mesh), and gradient eluted with an organic solvent consisting of ethyl acetate and methanol in a volume ratio of 89:11 and 83:17 (the elution volume was 6 BV and the flow rate was 2 BV / h), and the eluate eluted with the organic solvent consisting of ethyl acetate and methanol in a volume ratio of 89:11 was collected and dried to obtain a grape seed extract with a moisture content of <5%.

[0073] Comparative Example 6

[0074] The difference from Example 2 is that the grape seed extract is prepared by the following method, and the rest is the same:

[0075] S1, heating and refluxing the grape seeds with an ethanol aqueous solution having a volume concentration of 50%, the conditions for the heating and refluxing extraction being: a solid-liquid ratio of 1:15, an extraction time of 2 hours, and drying the extract to obtain a crude grape seed extract;

[0076] S2, applying the crude grape seed extract obtained in step S1 to a H103 macroporous resin column, and eluting with 25% (elution volume 4BV, flow rate 2BV / h) and 55% ethanol aqueous solution (elution volume 10BV, flow rate 2BV / h) in a gradient manner, collecting the eluate eluted with 55% ethanol aqueous solution, and drying to obtain a grape seed eluate;

[0077] S3. The grape seed eluate obtained in step S2 is loaded onto a silica gel column (FCP, 120 mesh), and gradient eluted with organic solvents consisting of ethyl acetate and methanol in a volume ratio of 82:18 and 78:22, respectively (the elution volume is 6 BV, and the flow rate is 2 BV / h). The eluate eluted with the organic solvent consisting of ethyl acetate and methanol in a volume ratio of 78:22 is collected, and dried to obtain a grape seed extract with a moisture content of <5%.

[0078] Results test:

[0079] 1. Antioxidant Capacity

[0080] 1.1 Determination of DPPH free radical scavenging ability

[0081] DPPH was prepared with anhydrous ethanol to 2×10 -4 mol / L solution, the embodiment and comparative example are used as the liquid to be tested, and several portions of 2 mL of the liquid to be tested, anhydrous ethanol, and DPPH solution are prepared. After mixing, they are placed at room temperature for 30 minutes, and the absorbance is measured at a wavelength of 517 nm to obtain Ai. Each sample is measured in parallel 3 times and the average value is taken. Vc is used as a positive control, and the absorbance value is measured by replacing DPPH with anhydrous ethanol as a control Aj blank, and the absorbance Ac is measured by mixing DPPH solution and anhydrous ethanol.

[0082] Clearance (%) = (1-(Ai-Aj) / Ac) × 100%

[0083] Wherein: Ai is the absorbance of 2 mL of the example or comparative example solution + 2 mL of the DPPH reagent mixture; Aj is the absorbance of 2 mL of the sample solution + 2 mL of anhydrous ethanol mixture; Ac is the absorbance of 2 mL of the DPPH solution + 2 mL of anhydrous ethanol mixture.

[0084] 1.2 Determination of superoxide radical scavenging ability

[0085] The embodiment and comparative example were respectively used as the liquid to be tested, 1 mL of the liquid to be tested was accurately drawn, 4.5 mL of 50 mmol / L Tris-HCl buffer with pH = 8.2 was added, and then mixed, kept in a constant temperature water bath at 25°C for 20 minutes, and then taken out, 0.3 mL of 25 mmol / L pyrogallol solution was immediately added, and the mixture was reacted in a constant temperature water bath at 25°C for 5 minutes after being quickly shaken, and then 1 mL of 8 mmol / L HCl was added, and the reaction was terminated after mixing, and the absorbance was measured at 325 nm, and distilled water was used instead of the sample as a blank control. Vc was used as a positive control. Each sample was measured in parallel 3 times, and the average value was taken.

[0086] Clearance rate (%) = (A blank - A test solution) / A blank × 100%

[0087] 1.3 Determination of hydroxyl radical scavenging ability

[0088] The examples and comparative examples were used as the test solution, and 1 mL 0.15 mmol / LFeSO4, 0.4 mL 2 mmol / L salicylic acid, 0.2 mL test solution, 1 mL 6 mmol / L H2O2 and 0.4 mL distilled water were added to the test tubes of the sample group respectively; the blank group used distilled water instead of the test solution, and the control group used distilled water instead of salicylic acid, and reacted at 37°C for 30 minutes. Vc was used as a positive control. Each sample was measured in parallel 3 times, and the average value was taken.

[0089] Clearance rate (%) = (A blank - (A test - A control)) / A blank × 100%

[0090] The results are as follows:

[0091]

[0092] 2. Anti-glycation ability

[0093] Inhibition of non-enzymatic glycation reaction test

[0094] Glucose solution and bovine serum albumin solution were prepared with PBS solution to make the final concentrations 200mmol / L and 40g / L, respectively. Take the example composition and the comparative example composition, and set up control groups at the same time: ① Group A, non-enzymatic saccharification reaction group without the composition; ② Group B, bovine serum albumin control; ③ Group C, glucose control with the composition; ④ Group D, bovine serum albumin control with the composition, and each group was repeated 3 times. Incubate at 37℃ in the dark for 30 days, and use a fluorescence spectrophotometer to measure the fluorescence value F of each group of reaction solution under the conditions of excitation wavelength 370nm, emission wavelength 440nm, and slit 1nm. Each sample was measured in parallel 3 times, and the average value was taken.

[0095]

[0096] The results are as follows:

[0097]

[0098] The results show that the composition prepared by the embodiment of the present invention has good antioxidant and anti-glycation effects, among which, from the results, embodiment 2 is the best formula. Comparative Examples 1 and 3 show that when the Agaricus bisporus extract is not added, the corresponding antioxidant and anti-glycation effects are significantly reduced. In this formula, the Agaricus bisporus extract and other ingredients have a synergistic effect. Comparative Example 2 shows that when the ratio is inappropriate, the corresponding antioxidant and anti-glycation effects are reduced. Comparative Examples 3, 4, 5, and 6 show that the optimization of the extraction method for grape seeds in the present invention, after the grape seed extract and other ingredients form a formula, significantly improves the antioxidant and anti-glycation effects of the entire formula.

[0099] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A composition for resisting glycation and delaying aging, characterized in that: The raw materials are composed of the following ingredients by weight: 1-10 parts of ginger powder, 1-10 parts of mulberry powder, 0.1-10 parts of sea buckthorn powder, 1-20 parts of bamboo leaf powder, 1-15 parts of grape seed extract, 1-10 parts of Agaricus bisporus extract and 1-13 parts of Haematococcus pluvialis astaxanthin microcapsule powder; The grape seed extract is prepared according to the following method: S1, extracting grape seeds by heating and refluxing with a solvent, and drying the extract to obtain a crude grape seed extract; S2, applying the crude grape seed extract obtained in step S1 to a macroporous resin column, and eluting with ethanol aqueous solution with a volume fraction of 20-30% and 50-60% in sequence, collecting the eluate eluted with the volume fraction of 50-60% ethanol aqueous solution, and drying to obtain a grape seed eluate; S3. The grape seed eluate obtained in step S2 is placed on a silica gel column, and gradient eluted with an organic solvent consisting of ethyl acetate and methanol in a volume ratio of 89:11 and 83:17, respectively, and the eluate eluted with the organic solvent consisting of ethyl acetate and methanol in a volume ratio of 83:17 is collected and dried to obtain a grape seed extract.

2. The anti-glycation and anti-aging composition according to claim 1, characterized in that: The raw materials are composed of the following ingredients by weight: 1-3 parts of ginger powder, 2-8 parts of mulberry powder, 0.5-5 parts of sea buckthorn powder, 2-8 parts of bamboo leaf powder, 2-10 parts of grape seed extract, 2-8 parts of Agaricus bisporus extract and 2-8 parts of Haematococcus pluvialis astaxanthin microcapsule powder.

3. The anti-glycation and anti-aging composition according to claim 2, characterized in that: The raw materials are composed of the following ingredients by weight: 1-2 parts of ginger powder, 4-6 parts of mulberry powder, 1-2 parts of sea buckthorn powder, 4-5 parts of bamboo leaf powder, 3-6 parts of grape seed extract, 3-4 parts of Agaricus bisporus extract and 5-6 parts of Haematococcus pluvialis astaxanthin microcapsule powder.

4. The anti-glycation and anti-aging composition according to claim 3, characterized in that: The raw materials are composed of the following ingredients in parts by weight: 1.5 parts of ginger powder, 4 parts of mulberry powder, 2 parts of sea buckthorn powder, 5 parts of bamboo leaf powder, 4 parts of grape seed extract, 4 parts of Agaricus bisporus extract and 5 parts of Haematococcus pluvialis astaxanthin microcapsule powder.

5. A method for preparing the anti-glycation and anti-aging composition according to any one of claims 1 to 4, characterized in that: Includes steps: (1) Mix ginger powder, mulberry powder, sea buckthorn powder, and bamboo leaf powder in the prescribed amount, and sieve to obtain a mixture; (2) mixing the mixture obtained in step (1) with a solvent, extracting, combining the extracts, concentrating, and drying to obtain an extract; (3) The extract obtained in step (2) is mixed with a formulated amount of grape seed extract, Agaricus bisporus extract, and Haematococcus pluvialis astaxanthin microcapsule powder to obtain the anti-glycation and anti-aging composition.

6. The preparation method according to claim 5, characterized in that: In step (2), the solvent is water or ethanol aqueous solution; the extraction conditions are: solid-liquid ratio 1:8-15, extraction time 1-4h, extraction times 2-4 times.

7. The preparation method according to claim 5, characterized in that: The grape seed extract is prepared according to the following method: S1, extracting grape seeds by heating and refluxing with a solvent, and drying the extract to obtain a crude grape seed extract; S2, applying the crude grape seed extract obtained in step S1 to a macroporous resin column, and eluting with ethanol aqueous solution with a volume fraction of 20-30% and 50-60% in sequence, collecting the eluate eluted with the volume fraction of 50-60% ethanol aqueous solution, and drying to obtain a grape seed eluate; S3. The grape seed eluate obtained in step S2 is placed on a silica gel column, and gradient eluted with an organic solvent consisting of ethyl acetate and methanol in a volume ratio of 89:11 and 83:17, respectively, and the eluate eluted with the organic solvent consisting of ethyl acetate and methanol in a volume ratio of 83:17 is collected and dried to obtain a grape seed extract.

8. The preparation method according to claim 7, characterized in that: In step S1, the solvent is an ethanol aqueous solution with a volume concentration of 40-60%, and the conditions for the heating reflux extraction are: a solid-liquid ratio of 1:10-20, and an extraction time of 1.5-3h.

9. The preparation method according to claim 7, characterized in that: In step S2, gradient elution is performed with ethanol aqueous solutions having volume fractions of 25% and 55% in sequence, and the eluate eluted with the ethanol aqueous solution having volume fraction of 55% is collected.

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