A potassium persulfate complex disinfectant and preparation method thereof

Abstract

The invention provides a potassium persulfate complex disinfectant and a preparation method thereof, belonging to the technical field of disinfectant preparation; the complex disinfectant comprises potassium persulfate, straw extract, sorbitol and PBS buffer. The complex disinfectant does not use sodium chloride and organic acid, has the advantages of short-time onset and good effect; and the disinfectant does not increase the burden on the use environment when used, and has the advantage of being environmentally friendly.

Description

Technical Field

[0001] The invention relates to the technical field of disinfectant preparation, and in particular to a potassium persulfate complex disinfectant and a preparation method thereof. Background Art

[0002] Disinfectants are widely used products in daily life and breeding. They can kill pathogenic microorganisms, cut off the transmission routes of pathogens, and achieve the purpose of controlling infection.

[0003] There are many kinds of disinfectants at present, among which potassium persulfate disinfectants are the most widely used. In water, potassium persulfate can undergo a chain reaction to generate free radicals and new ecological oxygen, change the permeability of bacterial cell membranes, and then achieve the purpose of sterilization and disinfection; the current potassium persulfate disinfectants often add sodium chloride and organic acids when compounding, and these two types of compounds will not decompose by themselves in water, that is, when the number of uses increases, sodium chloride may accumulate, which is easy to affect the water body and thus affect the smooth progress of breeding; and when potassium persulfate disinfectants containing sodium chloride and organic acids are sprayed on the surface of objects, as time goes by, salt will also accumulate on the surface of objects, which is easy to affect the surface of objects; therefore, it is of great significance to the industry to provide a potassium persulfate compound disinfectant that can not only improve the performance of potassium persulfate disinfectants, but also avoid the impact on the environment after use. Summary of the invention

[0004] In view of this, the present invention provides a potassium persulfate complex disinfectant and a preparation method thereof, wherein the complex disinfectant comprises potassium persulfate, straw extract, sorbitol and PBS buffer. The complex disinfectant has the advantages of short-term effectiveness and good effect; and the disinfectant does not increase the burden on the use environment when used, and has the advantage of being environmentally friendly.

[0005] The technical solution of the present invention is as follows:

[0006] A potassium persulfate complex disinfectant, comprising 50-55 parts of potassium persulfate, 25-30 parts of straw extract, 2-5 parts of sorbitol and PBS buffer;

[0007] The straw extract is prepared by:

[0008] (1) Crush the straw, add water, and the weight ratio of straw to water is 25-30:100, and stir thoroughly to obtain slurry;

[0009] (2) The pH of the slurry is adjusted to 6.5±0.1, and then cellulase, hemicellulase and pectinase are added thereto, the amount of cellulase added is 8.0-8.6u / g, the amount of hemicellulase added is 2.5-3.3u / g, and the amount of pectinase added is 4.0-4.4u / g. The slurry is heated to 52±1°C, maintained for 4.8-5.5h, sterilized, and an enzymatic hydrolyzate is obtained. The filtrate A is obtained by filtration, and the filtrate A is evaporated to obtain a paste A.

[0010] (3) Dissolving paste A with anhydrous ethanol and filtering to obtain filtrate B, evaporating filtrate B to obtain paste B; repeating the dissolving, filtering and evaporating process twice to obtain paste E; drying paste E to obtain straw extract; the straw extract mainly contains anthocyanins and anthocyanidins.

[0011] Preferably, the above-mentioned potassium persulfate complex disinfectant includes 53 parts of potassium persulfate, 27 parts of straw extract, 4 parts of sorbitol and 16 parts of PBS buffer.

[0012] Preferably, the pH of the PBS buffer is 6.5.

[0013] Preferably, in step (1), the straw is one or more combinations of tomato stems and leaves, pepper stems and leaves, and sugarcane straw.

[0014] Preferably, the straw is fresh straw, the particle size of the crushed straw is 2-4 mm, and the weight ratio of straw to water is 25:100.

[0015] Preferably, in step (3), formic acid is added dropwise to anhydrous ethanol, with 5-6 drops of formic acid added to every 100 ml of anhydrous ethanol.

[0016] The preparation method of the above-mentioned potassium persulfate complex disinfectant is as follows:

[0017] Step 1, mixing sorbitol with PBS buffer, adding straw extract after sorbitol is dissolved, and performing ultrasonic treatment until dissolved to obtain a mixed solution;

[0018] Step 2: Add potassium persulfate to the mixed solution and stir evenly to obtain a composite disinfectant.

[0019] Preferably, the ultrasonic dissolution conditions are 50-60 KHz, and ultrasound for 3-5 seconds.

[0020] Compared with the prior art, the beneficial effects of the present invention are:

[0021] (1) After the straw extract used in the present invention is compounded with potassium persulfate, the obtained disinfectant can effectively sterilize and eliminate Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Aeromonas hydrophila and Aeromonas sobria. After 20 seconds of action, the sterilization rate of Escherichia coli is 99.7%, the sterilization rate of Staphylococcus aureus is 99.5%, the sterilization rate of Pseudomonas aeruginosa is 99.4%, the sterilization rate of Aeromonas hydrophila is 99.5%, and the sterilization rate of Aeromonas sobria is 99.3%;

[0022] (2) The straw extract used in the disinfectant of the present invention not only makes resource utilization of straw waste, but also the obtained extract is mainly anthocyanin and anthocyanidin, which can cooperate with potassium persulfate to effectively eliminate pathogenic bacteria, and will not have adverse effects on water bodies, surfaces of objects, etc., and is safer to use and conducive to long-term use;

[0023] (3) The disinfectant of the present invention has the advantages of short-term effectiveness and good effect; and the disinfectant does not increase the burden on the use environment when used, and has the advantage of being environmentally friendly. DETAILED DESCRIPTION

[0024] In order to enable those skilled in the art to better understand the technical solutions of the present invention, the technical solutions of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work should fall within the scope of protection of the present invention.

[0025] Example 1

[0026] A potassium persulfate complex disinfectant, comprising 53 parts of potassium persulfate, 27 parts of straw extract, 4 parts of sorbitol and 16 parts of PBS buffer;

[0027] Among them, the pH of PBS buffer is 6.5;

[0028] The straw extract is prepared by:

[0029] (1) crushing straw, wherein the straw is tomato stems and leaves and pepper stems and leaves in a weight ratio of 1:1; the tomato stems and leaves and pepper stems and leaves are all fresh straw, and the crushed straw particle size is 2-4 mm, adding water, and the weight ratio of straw to water is 25:100, and fully stirring to obtain a slurry;

[0030] (2) The pH of the slurry was adjusted to 6.5, and then cellulase, hemicellulase and pectinase were added thereto, with the added amount of cellulase being 8.1 u / g, the added amount of hemicellulase being 2.7 u / g, and the added amount of pectinase being 4.3 u / g. The slurry was heated to 52°C and maintained for 5.0 h, sterilized, and an enzymatic hydrolyzate was obtained. The filtrate A was obtained by filtration, and the filtrate A was evaporated to obtain a paste A.

[0031] (3) dissolving paste A in anhydrous ethanol and filtering to obtain filtrate B, evaporating filtrate B to obtain paste B;

[0032] Use a rubber-tipped dropper to add formic acid to the anhydrous ethanol, adding 5 drops of formic acid per 100 ml of anhydrous ethanol;

[0033] Repeat the dissolving, filtering and evaporating process twice to obtain paste E; dry the paste E to obtain straw extract; the straw extract mainly contains anthocyanin and anthocyanidin;

[0034] The preparation method of the above-mentioned potassium persulfate complex disinfectant is as follows:

[0035] Step 1, mixing sorbitol with PBS buffer, adding straw extract after sorbitol is dissolved, and ultrasonicating until dissolved to obtain a mixed solution; the ultrasonic dissolution condition is 55KHz, and the ultrasonication is 3s;

[0036] Step 2: Add potassium persulfate to the mixed solution and stir evenly to obtain a composite disinfectant.

[0037] Comparative Example 1

[0038] The difference from Example 1 is that step (3) is not performed, and the obtained paste A is the straw extract.

[0039] Comparative Example 2

[0040] The difference from Example 1 is that the straw extract is prepared by the prior art method, which is:

[0041] The straw is crushed into tomato stems and leaves and pepper stems and leaves in a weight ratio of 1:1; the tomato stems and leaves and pepper stems and leaves are all fresh straw, and the crushed straw particle size is 2-4mm, placed in a pot, added with water and boiled, the weight ratio of straw to water is 1:10, boiled, kept for 3 hours, repeated three times, the three extracts were combined, and evaporated to obtain a straw extract.

[0042] Comparative Example 3

[0043] A cleaning disinfectant is prepared according to the patent document with application number 202110571779.2 and subject name "A cleaning disinfectant containing potassium persulfate complex and its use method and application".

[0044] Example 2

[0045] A potassium persulfate complex disinfectant, comprising 50 parts of potassium persulfate, 25 parts of straw extract, 2 parts of sorbitol and 23 parts of PBS buffer;

[0046] Among them, the pH of PBS buffer is 6.5;

[0047] The straw extract is prepared by:

[0048] (1) The straw is tomato stems and leaves and pepper stems and leaves in a weight ratio of 1:1; the tomato stems and leaves and pepper stems and leaves are all fresh straws, the straw particles after crushing have a diameter of 2-4 mm, water is added, the weight ratio of straw to water is 30:100, and slurry is obtained after sufficient stirring;

[0049] (2) The pH of the slurry was adjusted to 6.4, and then cellulase, hemicellulase and pectinase were added thereto, with the addition amount of cellulase being 8.0 u / g, the addition amount of hemicellulase being 2.5 u / g, and the addition amount of pectinase being 4.0 u / g. The slurry was heated to 51°C and maintained for 5.5 h, sterilized, and an enzymatic hydrolyzate was obtained. The filtrate A was obtained by filtration, and the filtrate A was evaporated to obtain a paste A.

[0050] (3) dissolving paste A in anhydrous ethanol and filtering to obtain filtrate B, evaporating filtrate B to obtain paste B;

[0051] Use a rubber-tipped dropper to add formic acid to the anhydrous ethanol, adding 6 drops of formic acid per 100 ml of anhydrous ethanol;

[0052] Repeat the dissolving, filtering and evaporating process twice to obtain paste E; dry the paste E to obtain straw extract; the straw extract mainly contains anthocyanin and anthocyanidin;

[0053] The preparation method of the above-mentioned potassium persulfate complex disinfectant is as follows:

[0054] Step 1, mixing sorbitol with PBS buffer, adding straw extract after sorbitol is dissolved, and performing ultrasonic treatment until dissolved to obtain a mixed solution;

[0055] Ultrasonic dissolution conditions were 50 KHz, 3 s;

[0056] Step 2: Add potassium persulfate to the mixed solution and stir evenly to obtain a composite disinfectant.

[0057] Example 3

[0058] A potassium persulfate complex disinfectant, comprising 55 parts of potassium persulfate, 30 parts of straw extract, 5 parts of sorbitol and 10 parts of PBS buffer;

[0059] Among them, the pH of PBS buffer is 6.5;

[0060] The straw extract is prepared by:

[0061] (1) The straw is tomato stems and leaves and pepper stems and leaves in a weight ratio of 1:1; the tomato stems and leaves and pepper stems and leaves are all fresh straws, the straw particles after crushing have a diameter of 2-4 mm, water is added, the weight ratio of straw to water is 27:100, and slurry is obtained after sufficient stirring;

[0062] (2) The pH of the slurry was adjusted to 6.6, and then cellulase, hemicellulase and pectinase were added thereto, with the added amount of cellulase being 8.6 u / g, the added amount of hemicellulase being 3.3 u / g, and the added amount of pectinase being 4.4 u / g. The slurry was heated to 53°C and maintained for 4.8 h, sterilized, and an enzymatic hydrolyzate was obtained. The filtrate A was obtained by filtration, and the filtrate A was evaporated to obtain a paste A.

[0063] (3) dissolving paste A in anhydrous ethanol and filtering to obtain filtrate B, evaporating filtrate B to obtain paste B;

[0064] Use a rubber-tipped dropper to add formic acid to the anhydrous ethanol, adding 5 drops of formic acid per 100 ml of anhydrous ethanol;

[0065] Repeat the dissolving, filtering and evaporating process twice to obtain paste E; dry the paste E to obtain straw extract; the straw extract mainly contains anthocyanin and anthocyanidin;

[0066] The preparation method of the above-mentioned potassium persulfate complex disinfectant is as follows:

[0067] Step 1, mixing sorbitol with PBS buffer, adding straw extract after sorbitol is dissolved, and performing ultrasonic treatment until dissolved to obtain a mixed solution;

[0068] Ultrasonic dissolution conditions were 60 KHz, 5 s;

[0069] Step 2: Add potassium persulfate to the mixed solution and stir evenly to obtain a composite disinfectant.

[0070] Example 4

[0071] The difference from Example 1 is that the composition of the composite disinfectant is different, and the composition of the straw is different, as follows:

[0072] Potassium persulfate complex disinfectant, including 52 parts of potassium persulfate, 28 parts of straw extract, 3 parts of sorbitol and 17 parts of PBS buffer;

[0073] The straw is the stems and leaves of fresh tomatoes.

[0074] Example 5

[0075] The difference from Example 1 is that

[0076] The difference from Example 1 is that the composition of the composite disinfectant is different, and the composition of the straw is different, as follows:

[0077] A potassium persulfate complex disinfectant, comprising 51 parts of potassium persulfate, 25 parts of straw extract, 4 parts of sorbitol and 15 parts of PBS buffer;

[0078] The straw is fresh sugarcane straw.

[0079] Example 6

[0080] The difference from Example 1 is that the composition of the composite disinfectant is different, as follows:

[0081] A potassium persulfate complex disinfectant comprises 54 parts of potassium persulfate, 26 parts of straw extract, 4 parts of sorbitol and 16 parts of PBS buffer.

[0082] Sterilization test

[0083] The disinfectants of Examples 1-6 and Comparative Examples 1-3 of the present invention are all prepared and used immediately.

[0084] (1) Cultivation

[0085] Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa were cultured in LB medium for 36 h;

[0086] Aeromonas hydrophila and Aeromonas sobria were cultured in beef extract peptone medium for 36 h;

[0087] The culture conditions and the preparation of the culture medium are all prior art and will not be described in detail in this application;

[0088] (2) Preparation of test solution

[0089] Scrape the bacterial moss on the above culture medium, shake it with sterile physiological saline, centrifuge it twice, and then dilute it logarithmically with 30g / L bovine serum albumin solution to prepare a test solution;

[0090] Take ten sterile test tubes, add 1 ml of each of the above test solutions respectively, and then add 2 ml of the disinfectants of Examples 1-6 and Comparative Examples 1-3 respectively, and the control group is PBS buffer (pH 6.5); after 20 seconds, a treated solution is obtained;

[0091] Take 0.5 ml of the treated solution, add 4.5 ml of neutralizer (0.3% sodium thiosulfate) into the test tube, mix well, neutralize for 15 minutes, take samples and inoculate them for live bacteria technology, calculate the killing logarithm, repeat the test 5 times, the results are shown in Table 1 and Table 2, as follows:

[0092] Table 1 Results of logarithmic determination of bacterial counts in the control group

[0093]

[0094] Table 2 Example and comparative example disinfectant killing logarithmic value measurement results

[0095]

[0096] Table 3 Sterilization rate relative to the control group

[0097]

[0098] Combining the results of Example 1 with those of Comparative Example 3, it can be seen that the sterilization effect of the disinfectant provided by the present invention is slightly better than that of the disinfectant in Comparative Example 3 (prior art); under the premise of equivalent sterilization levels, the present invention makes resource utilization of straw waste, which has the advantage of being environmentally friendly; in addition, the disinfectant of the present invention does not use sodium chloride and organic acid, so when used in water or on the surface of an object, it will not have an adverse effect on the water body or the surface of an object, and is more practical and environmentally friendly;

[0099] Combining the results of Examples 1 to 6, it can be seen that the disinfectant of the present invention has a good disinfecting effect on bacteria, and the treatment effect is stable;

[0100] Combining the results of Example 1 and Comparative Example 1, it can be seen that the sterilization effect of the straw extract is significantly reduced when it is not treated in step (3). This may be because the paste A contains polysaccharides, monosaccharides and other substances in addition to anthocyanins and anthocyanidins. The presence of these substances leads to a decrease in the performance of the disinfectant.

[0101] Combining the results of Example 1 and Comparative Example 2, it can be seen that the different extraction processes affect the composition of the straw extract, and the different composition of the straw extract affects the performance of the disinfectant.

[0102] In summary, the composite disinfectant provided by the present invention has the advantages of short-term effectiveness and good effect; it can cooperate with potassium persulfate to effectively eliminate pathogenic bacteria, and will not have adverse effects on water bodies, object surfaces, etc., and is safer to use and is conducive to use over a long period of time.

[0103] Although the present invention has been described in detail by reference to the preferred embodiments, the present invention is not limited thereto. Without departing from the spirit and essence of the present invention, a person of ordinary skill in the art may make various equivalent modifications or substitutions to the embodiments of the present invention, and these modifications or substitutions shall be within the scope of the present invention. Any person of ordinary skill in the art may easily think of changes or substitutions within the technical scope disclosed by the present invention, and these shall be within the scope of protection of the present invention. Therefore, the scope of protection of the present invention shall be subject to the scope of protection of the claims.

Claims

1. A potassium persulfate complex disinfectant, characterized in that: It includes 53 parts of potassium persulfate, 27 parts of straw extract, 4 parts of sorbitol and 16 parts of PBS buffer; The straw extract is prepared by: (1) Crush the straw, add water, and the weight ratio of straw to water is 25-30:100, and stir thoroughly to obtain slurry; The straw is one or more combinations of tomato stems and leaves, pepper stems and leaves, and sugarcane straw; (2) The pH of the slurry is adjusted to 6.5±0.1, and then cellulase, hemicellulase and pectinase are added thereto, the amount of cellulase added is 8.0-8.6u / g, the amount of hemicellulase added is 2.5-3.3u / g, and the amount of pectinase added is 4.0-4.4u / g. The slurry is heated to 52±1°C, maintained for 4.8-5.5h, sterilized, and an enzymatic hydrolyzate is obtained. The filtrate A is obtained by filtration, and the filtrate A is evaporated to obtain a paste A. (3) Dissolving paste A with anhydrous ethanol and filtering to obtain filtrate B, evaporating filtrate B to obtain paste B; repeating the dissolving, filtering and evaporating process twice to obtain paste E; drying paste E to obtain straw extract; the straw extract mainly contains anthocyanins and anthocyanidins; Formic acid is added dropwise to anhydrous ethanol, with 5-6 drops of formic acid added to every 100 ml of anhydrous ethanol.

2. The potassium persulfate complex disinfectant according to claim 1, characterized in that The pH of the PBS buffer is 6.

5.

3. The potassium persulfate complex disinfectant according to claim 1, characterized in that: The straw is fresh straw, the particle size of the crushed straw is 2-4 mm, and the weight ratio of straw to water is 25:

100.

4. The method for preparing the potassium persulfate complex disinfectant according to claim 1, wherein: The process is as follows: Step 1, mixing sorbitol with PBS buffer, adding straw extract after sorbitol is dissolved, and performing ultrasonic treatment until dissolved to obtain a mixed solution; Step 2: Add potassium persulfate to the mixed solution and stir evenly to obtain a composite disinfectant.

5. The method for preparing the potassium persulfate complex disinfectant according to claim 4, characterized in that: The ultrasonic dissolution conditions are 50-60KHz, ultrasound 3-5s.

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