Herb beauty oil with soothing and repairing effects and preparation method thereof

Abstract

The present invention discloses a herbal beauty oil with soothing and repairing effects and a preparation method thereof. Currently, most of the active ingredients for skin allergies such as plant extracts and peptides are added to the formula in the industry to prevent and relieve allergies. Oil-soluble ingredients are easier to penetrate the skin than water-soluble ingredients to exert their effects, but there are few oil-soluble soothing and repairing ingredients on the market. The present invention combines Stemona, Lithospermum officinale and Lanterna in a very certain range of proportions, extracts them through a specific process, and obtains a herbal beauty oil, which has the effects of clearing heat and cooling blood, removing dampness and relieving itching, promoting blood circulation and detoxifying, and is used in cosmetics to have a good soothing and repairing effect on sensitive skin.

Description

Technical Field

[0001] The present invention relates to the field of cosmetics, and in particular to a herb beauty oil with soothing and repairing effects and a preparation method thereof. Background Art

[0002] With the rapid development of the cosmetics industry and the continuous development of the cosmetics manufacturing industry, the types of raw materials that can be used to make cosmetics are increasing, and the number of raw materials used in cosmetic formulas is also increasing, so the safety risks of cosmetics are getting higher and higher. According to the data from the Cosmetic Adverse Reaction Monitoring Network of the National Health and Family Planning Commission of my country (formerly the National Health and Family Planning Commission), contact dermatitis caused by cosmetic allergies or irritation accounts for more than 90% of cosmetic skin diseases.

[0003] In order to reduce the safety risks of cosmetic production and use as much as possible and make the products more gentle, major cosmetic companies have added plant extracts, active peptides and other active ingredients to prevent and relieve skin allergies in their formulas. For example, French L'Oreal cosmetics add chamomile to the formula to improve the skin's immune defense ability, and European and American countries add North American witch hazel extract to cosmetics to promote the repair of skin allergy dermatitis.

[0004] However, most of the soothing and repairing plant extracts on the market are water-soluble raw materials, while oil-soluble soothing and repairing plant extracts are relatively rare. From the perspective of transdermal absorption, in the absence of skin lesions, oil-soluble ingredients are easier to penetrate the skin than water-soluble ingredients. Therefore, it is of great significance to study oil-soluble plant extracts with soothing and repairing effects.

[0005] After extensive research in the field of cosmetics, the inventors of the present invention discovered that by combining Stemona bulbs, Lithospermum officinale and Glehnia littoralis in a very specific range of proportions and extracting them through a specific process, they obtained a herbal beauty oil that has the effects of clearing heat and cooling blood, removing dampness and relieving itching, promoting blood circulation and detoxifying. The oil can be used in cosmetics to soothe and repair sensitive skin, thus completing the present invention. Summary of the invention

[0006] In view of this, the present invention provides an oil-soluble herb beauty oil, which is a combination of Stemona japonica, Lithospermum officinale and Lantern Festival in a very specific range of proportions and is extracted through a specific process. It has the effects of clearing heat and cooling blood, removing dampness and relieving itching, promoting blood circulation and detoxifying. Its application in cosmetics can have good soothing and repairing effects on sensitive skin.

[0007] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0008] A herbal beauty oil with soothing and repairing effects, characterized in that it comprises the following components: STEMONA TUBEROSA root extract, LITHOSPERMUM ERYTHRORHIZON root extract, and CARDIOSPERMUM HALICACABUM flower / leaf / vine extract.

[0009] The Stemona ulmoides Lour. of the present invention is also called the large Stemona ulmoides, and is the root of the Stemona ulmoides Lour. of the Stemonaceae family. It has the effects of moistening the lungs, lowering qi, relieving cough, killing insects and killing lice. It is commonly used for new and long-term coughs, coughs caused by tuberculosis, and sudden coughs; it is externally used for head lice, body lice, pinworm disease, and vaginal itching. Honey Stemona ulmoides moistens the lungs and relieves coughs, and is used for yin deficiency and fatigue coughs.

[0010] The lithospermum described in the present invention is the root of Lithospermum erythrorhizon Siebold & Zucc., a plant of the genus Lithospermum of the Boraginaceae family. Lithospermum has a long history of cultivation in China. It was first widely planted as a dye crop, and then the medicinal value of lithospermum was gradually developed. The Shennong's Herbal Classic describes the medicinal value of lithospermum: "It tastes bitter and cold. It cures evil qi in the heart and abdomen, five jaundices, replenishes the middle and replenishes qi, benefits the nine orifices, and clears the water channels."

[0011] The Cardiospermum halicacabum described in the present invention is the above-ground part of the herbaceous climbing vine Cardiospermum halicacabum L. of the Sapindaceae family, including flowers / leaves / vines. The whole Cardiospermum halicacabum plant can be used as medicine, has a bitter taste and is cold in nature. It has the effects of clearing away heat and dampness, cooling blood and detoxifying, and can be used to treat jaundice, gonorrhea, furuncle, blister sore, scabies, snake bites and other diseases.

[0012] The embodiment of the present invention discloses a preparation process of the herb beauty oil, and the specific steps are as follows:

[0013] (1) Weigh the following raw materials in parts by weight: 1-10 parts of Stemona tuberosa root, 1-10 parts of Lithospermum officinale root, and 1-10 parts of the aerial part of Cyperus rotundus, and grind and sieve to obtain a mixed powder;

[0014] (2) preparing 75-99% V / V ethanol aqueous solution as solvent A, mixing the mixed powder and solvent A at a weight ratio of 1:3-5, and soaking to obtain a soaked material;

[0015] (3) loading the soaked material into a chromatography column and eluting with solvent A to obtain 1-2 BV of eluate;

[0016] (4) adding an equal volume of oil to the eluate, mixing evenly, placing the mixture in an extraction tower and standing to separate layers to obtain a lower clear liquid;

[0017] (5) The lower clear liquid is evaporated to remove the residual ethanol, thereby obtaining the herbal beauty oil.

[0018] In one embodiment, the weight parts of the raw materials in step (1) are: 2 parts of Stemona odorata root, 5 parts of Lithospermum erythrorhizon root, and 5 parts of the aerial part of Cyperus rotundus.

[0019] In some embodiments, the sieving in step (1) is through a 100-mesh sieve.

[0020] In some embodiments, the concentration of the ethanol aqueous solution in step (2) is 95% V / V.

[0021] In some embodiments, the mixed powder and solvent A in step (2) are mixed in a weight ratio of 1:4.

[0022] The oil in step (4) can be animal oil, vegetable oil, or synthetic oil. In some embodiments, the oil is sunflower seed oil.

[0023] In some embodiments, the rotary evaporation temperature in step (5) is 48-55°C.

[0024] The herb beauty oil prepared in the embodiment of the present invention can be used as an active ingredient in cosmetic formulas. The cosmetics can be creams, lotions, facial masks, oils, etc.

[0025] The embodiment of the present invention also provides a repairing and nourishing essential oil, comprising the following components in percentage by weight: 30-50% squalane, 10-30% hydrogenated polyisobutene, 10-20% caprylic / capric triglyceride, 1-2% isododecane, 0.1-1% tocopherol (vitamin E), 1-10% of the above-mentioned herb beauty oil, and the balance grape seed oil.

[0026] The technical solution provided by the embodiment of the present invention has the following beneficial effects:

[0027] (1) The herb beauty oil prepared by the present invention adopts a plant formula, and its effect is better than that of a single prescription preparation;

[0028] (2) The preparation process of the herb beauty oil of the present invention mainly adopts the method of column chromatography + extraction, which can efficiently extract the active ingredients. The temperature of the whole process does not exceed 55°C. Compared with the conventional method of high-temperature frying of lithospermum, the heat-sensitive components are not destroyed. Experiments have shown that the application of it in cosmetics can have good soothing and repairing effects on sensitive skin. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for describing the embodiments or the prior art are briefly introduced below.

[0030] Figure 1 The VISIA-CR facial images of volunteers are shown. The red area image can reflect the aggregation of subcutaneous red blood cells on the face, and thus reflect the degree of facial sensitivity. The decrease in redness represents a decrease in sensitivity. DETAILED DESCRIPTION

[0031] The present invention discloses a herb beauty oil. Those skilled in the art can refer to the content of this article and appropriately improve the process parameters to achieve it. It should be particularly noted that all similar substitutions and modifications are obvious to those skilled in the art, and they are considered to be included in the present invention. The method and application of the present invention have been described through preferred embodiments, and relevant personnel can obviously modify or appropriately change and combine the methods and applications described herein without departing from the content, spirit and scope of the present invention to implement and apply the technology of the present invention.

[0032] The raw materials and reagents used in the herb beauty oil provided by the present invention can be purchased from the market.

[0033] The present invention will be further described below in conjunction with embodiments:

[0034] Example 1

[0035] A herb beauty oil is prepared according to the following preparation process:

[0036] (1) Weigh the following raw materials in parts by weight: 1 part of Stemona radix root, 10 parts of Lithospermum officinale root, and 1 part of the aerial part of Cyperus rotundus, and grind them through a 100-mesh sieve to obtain a mixed powder;

[0037] (2) preparing a 99% V / V ethanol aqueous solution as solvent A, mixing the mixed powder and solvent A in a weight ratio of 1:3, and soaking to obtain a soaked material;

[0038] (3) loading the soaked material into a chromatography column and eluting with solvent A to obtain 2 BV of eluate;

[0039] (4) adding an equal volume of octyl dodecanol to the eluate, mixing evenly, placing the eluate in an extraction tower and allowing it to stand for stratification to obtain a lower clear liquid;

[0040] (5) The lower clear liquid is evaporated at 48°C to remove the residual ethanol, thereby obtaining the herbal beauty oil.

[0041] Example 2

[0042] A herb beauty oil is prepared according to the following preparation process:

[0043] (1) Weigh the following raw materials in parts by weight: 10 parts of Stemona radix root, 1 part of Lithospermum officinale root, and 10 parts of the aerial part of Cyperus rotundus, and grind them through a 100-mesh sieve to obtain a mixed powder;

[0044] (2) preparing a 75% V / V ethanol aqueous solution as solvent A, mixing the mixed powder and solvent A at a weight ratio of 1:5, and soaking to obtain a soaked material;

[0045] (3) Loading the soaked material into a chromatography column and eluting with solvent A to obtain 1 BV of eluate;

[0046] (4) adding an equal volume of deep-sea fish oil to the eluate, mixing evenly, placing the mixture in an extraction tower and standing to separate layers to obtain a lower clear liquid;

[0047] (5) The lower clear liquid is evaporated at 55°C to remove the residual ethanol, thereby obtaining the herbal beauty oil.

[0048] Example 3

[0049] A herb beauty oil is prepared according to the following preparation process:

[0050] (1) Weigh the following raw materials in parts by weight: 2 parts of Stemona tuberosa root, 5 parts of Lithospermum officinale root, and 5 parts of the aerial part of Cyperus rotundus, and grind them through a 100-mesh sieve to obtain a mixed powder;

[0051] (2) preparing a 95% V / V ethanol aqueous solution as solvent A, mixing the mixed powder and solvent A in a weight ratio of 1:4, and soaking to obtain a soaked material;

[0052] (3) loading the soaked material into a chromatography column and eluting with solvent A to obtain 1.2 BV of eluate;

[0053] (4) adding an equal volume of sunflower seed oil to the eluate, mixing evenly, placing the mixture in an extraction tower and standing for stratification to obtain a lower clear liquid;

[0054] (5) The lower clear liquid is evaporated at 50°C to remove the residual ethanol, thereby obtaining the herbal beauty oil.

[0055] Comparative Example 1

[0056] A herb beauty oil is prepared according to the following preparation process:

[0057] (1) Weigh the following raw materials in parts by weight: 12 parts of Stemona tuberosa root, crush through a 100-mesh sieve to obtain a mixed powder;

[0058] (2) preparing a 95% V / V ethanol aqueous solution as solvent A, mixing the mixed powder and solvent A in a weight ratio of 1:4, and soaking to obtain a soaked material;

[0059] (3) loading the soaked material into a chromatography column and eluting with solvent A to obtain 1.2 BV of eluate;

[0060] (4) adding an equal volume of sunflower seed oil to the eluate, mixing evenly, placing the mixture in an extraction tower and standing for stratification to obtain a lower clear liquid;

[0061] (5) The lower clear liquid is evaporated at 50°C to remove the residual ethanol, thereby obtaining the herbal beauty oil.

[0062] Comparative Example 2

[0063] A herb beauty oil is prepared according to the following preparation process:

[0064] (1) Weigh the following raw materials in parts by weight: 12 parts of Lithospermum officinale root, crush through a 100-mesh sieve to obtain a mixed powder;

[0065] (2) preparing a 95% V / V ethanol aqueous solution as solvent A, mixing the mixed powder and solvent A in a weight ratio of 1:4, and soaking to obtain a soaked material;

[0066] (3) loading the soaked material into a chromatography column and eluting with solvent A to obtain 1.2 BV of eluate;

[0067] (4) adding an equal volume of sunflower seed oil to the eluate, mixing evenly, placing the mixture in an extraction tower and standing for stratification to obtain a lower clear liquid;

[0068] (5) The lower clear liquid is evaporated at 50°C to remove the residual ethanol, thereby obtaining the herbal beauty oil.

[0069] Comparative Example 3

[0070] A herb beauty oil is prepared according to the following preparation process:

[0071] (1) Weigh the following raw materials in parts by weight: 12 parts of the aerial part of the wormwood, crush through a 100-mesh sieve to obtain a mixed powder;

[0072] (2) preparing a 95% V / V ethanol aqueous solution as solvent A, mixing the mixed powder and solvent A in a weight ratio of 1:4, and soaking to obtain a soaked material;

[0073] (3) loading the soaked material into a chromatography column and eluting with solvent A to obtain 1.2 BV of eluate;

[0074] (4) adding an equal volume of sunflower seed oil to the eluate, mixing evenly, placing the mixture in an extraction tower and standing for stratification to obtain a lower clear liquid;

[0075] (5) The lower clear liquid is evaporated at 50°C to remove the residual ethanol, thereby obtaining the herbal beauty oil.

[0076] Comparative Example 4

[0077] A herb beauty oil is prepared according to the following preparation process:

[0078] (1) Weigh the following raw materials in parts by weight: 2 parts of Stemona tuberosa root, 5 parts of Lithospermum officinale root, and 5 parts of the aerial part of Cyperus rotundus, and grind them through a 100-mesh sieve to obtain a mixed powder;

[0079] (2) Add an equal volume of sunflower seed oil to the mixed powder, mix well, heat to 85° C., stir and extract for 1 hour, then centrifuge at 3000 rpm for 30 minutes, and take the supernatant to obtain the herb beauty oil.

[0080] Experiment 1: Soothing effect test

[0081] The Baicao beauty oil prepared in Examples 1-3 and Comparative Examples 1-3 was tested in accordance with the method described in the standard TSHRH033-2020 "Cosmetic Soothing Efficacy Test In Vitro - Determination of TNF-α Inflammatory Factor Content".

[0082] Principle: Bacterial lipopolysaccharide (LPS) binds to the antigen recognition receptor on the surface of macrophages, inducing macrophages to secrete TNF-α and other cytokines. TNF-α can activate three signaling pathways of Caspase protease, JNK and transcription factor NF-kB, realize its biological functions such as cytotoxicity, antiviral, immunomodulation and apoptosis, and is one of the important inflammatory factors. LPS induces RAW264.7, which is a classic cell model for studying inflammatory factors. By comparing the difference in the secretion of TNF-α by RAW264.7 after administration of the negative control and the test substance, the effect of the test substance on inhibiting the secretion of TNF-α is evaluated. The determination of TNF-α content adopts enzyme-linked immunosorbent assay (ELISA). The specific principle is: TNF-α specifically binds to the TNF-α antibody coated on the enzyme-labeled plate, and then binds to the anti-TNF-α antibody with substrate labeling. After the substrate is catalyzed by the enzyme, a colored product is generated. The TNF-α content is positively correlated with the color depth of the colored product. The optical density (OD) was measured at 450 nm using an enzyme marker and the TNF-α content was calculated.

[0083] Test steps:

[0084] (1) Cell digestion and inoculation. The concentration of trypsin is 0.25%, 1 mL of trypsin is used for T25 culture flasks, and 3 mL of trypsin is used for T75 culture flasks. Observe under a microscope. When most of the cells become round and suspended, add about 2-3 times the volume of serum-containing DMEM culture medium to terminate the digestion, and collect the cells into a centrifuge tube. Centrifuge at 800r / min for 6 minutes. The speed and centrifugation time can be determined according to the characteristics of the laboratory cells. After centrifugation, discard the supernatant, add a certain volume of cell culture medium to the centrifuge tube, and mix the cells by pipetting with an elbow pipette. Count the cells with a cell counter or a hemocytometer. Dilute the cells with cell culture medium to the inoculation density (the degree of fusion reaches 45% 24 hours after inoculation).～ 60%), inoculated into 96-well plates, with 200 μL per well. After inoculation, the plates were placed in a CO2 incubator and cultured for 24 h±2 h.

[0085] (2) Induction and administration: Discard the culture medium in the 96-well plate and carry out the induction and administration operation. Add the culture medium containing the test substance and LPS to the test substance wells, where the test substances are diluted to a concentration of 0.1% with dimethyl sulfoxide. Add cell culture medium containing LPS to the negative control wells and cell culture medium to the blank / solvent control wells, 200 μL per well. After the administration is completed, place the 96-well plate in a CO2 incubator and culture for 24 h ± 2 h.

[0086] (3) ELISA detection: After the incubation, the cell culture supernatant was collected and the TNF-α content in the test cell culture supernatant was determined using an ELISA kit.

[0087] Table 1 Soothing effect test results of Examples 1-3 and Comparative Examples 1-3

[0088] Experimental Group concentration TNF-α content Example 1 0.125％ 15.14±0.56 Example 2 0.125％ 18.36±0.04 Example 3 0.125％ 12.09±1.17 Comparative Example 1 0.125％ 56.56±1.84 Comparative Example 2 0.125％ 54.14±0.57 Comparative Example 3 0.125％ 69.58±3.13 Comparative Example 4 0.125％ 79.58±3.13

[0089] According to data table 1, the herb beauty oil prepared in Examples 1-3 of the present invention can effectively reduce the secretion of TNF-α by macrophages induced by LPS.

[0090] Example 4

[0091] A repairing and nourishing essential oil comprises the following components in percentage by weight: 30% squalane, 30% hydrogenated polyisobutene, 10% caprylic / capric triglyceride, 2% isododecane, 0.1% tocopherol (vitamin E), 10% herb beauty oil prepared in Example 1, and the balance grape seed oil.

[0092] Example 5

[0093] A repairing and nourishing essential oil comprises the following components in percentage by weight: 50% squalane, 10% hydrogenated polyisobutene, 20% caprylic / capric triglyceride, 1% isododecane, 1% tocopherol (vitamin E), 5% herb beauty oil prepared in Example 3, and the balance grape seed oil.

[0094] Experiment 2: Volunteer Trial Test

[0095] The effect of cosmetics on the human body is a very complex biological process. The evaluation of the efficacy of cosmetics requires comprehensive testing, scientific analysis and reasonable explanation from different angles and levels. Cosmetics are products that focus on experience, and consumer perception is very important. Consumer use testing refers to the process of effectively collecting, organizing and analyzing consumer product usage and efficacy claim evaluation information based on objective and scientific methods [1]. (Reference: [1] Liu Weiyi, Zhou Lin, & Zhao Hua. (2021). Cosmetic Efficacy Evaluation (ⅩⅢ)--Consumer Use Testing. Daily Chemical Industry, 51(6), 6.)

[0096] 30 volunteers were recruited to try the repairing and nourishing essential oil prepared in Example 5. Volunteer selection criteria: females aged 35-55 years old, volunteers with sensitive skin with symptoms such as itching, erythema, papules, blisters, etc. on the face, who had not used products related to the tested efficacy recently, and who were willing to participate and could complete the prescribed content according to the test requirements.

[0097] Specific usage: After cleansing, take an appropriate amount of the repairing and nourishing essential oil and gently circle it on the face until it is completely absorbed. Apply once in the morning and evening, and conduct a follow-up visit after continuous use for 3 months. Among them, the skin sensitivity symptoms of 18 volunteers were well relieved, the skin sensitivity symptoms of 7 volunteers were improved to a certain extent, and the symptoms of the remaining 5 volunteers were not improved. In general, after continuous use of the repairing and nourishing essential oil provided by the embodiment of the present invention for 3 months, the improvement rate of sensitive skin exceeded 80%, achieving good results.

[0098] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A herbal beauty oil with soothing and repairing effects, characterized in that: The preparation process includes the following steps: (1) Weigh the following raw materials in parts by weight: 1-10 parts of Stemona radix root, 1-10 parts of Lithospermum officinale root, and 1-10 parts of the aerial parts of Cyperus rotundus (flowers, leaves, and vines), and grind and sieve them to obtain a mixed powder; (2) preparing a 75-99% V / V ethanol aqueous solution as solvent A, mixing the mixed powder and solvent A in a weight ratio of 1:3-5, and soaking to obtain a soaked material; (3) Load the soaked material into a chromatography column and elute with solvent A to obtain 1-2 BV of eluate; (4) adding an equal volume of sunflower seed oil to the eluate, mixing the mixture evenly, placing the mixture in an extraction tower and allowing it to stand for stratification to obtain a lower clear liquid; (5) The lower clear liquid is evaporated to remove the residual ethanol to obtain the herbal beauty oil.

2. The preparation process of the herb beauty oil with soothing and repairing effects according to claim 1, characterized in that: The weight proportions of the raw materials in step (1) are: 2 parts of Stemona radix roots, 5 parts of Lithospermum officinale roots, and 5 parts of the aerial parts of Cyperus rotundus - flowers / leaves / vines.

3. The preparation process of the herb beauty oil with soothing and repairing effects according to claim 1, characterized in that: The sieving in step (1) is through a 100-mesh sieve.

4. The preparation process of the herb beauty oil with soothing and repairing effects according to claim 1, characterized in that: The concentration of the ethanol aqueous solution in step (2) is 95% V / V.

5. The preparation process of the herb beauty oil with soothing and repairing effects according to claim 1, characterized in that: The mixed powder and solvent A in step (2) are mixed in a weight ratio of 1:

4.

6. The preparation process of the herb beauty oil with soothing and repairing effects according to claim 1, characterized in that: The rotary evaporation temperature in step (5) is 48-55°C.

7. Use of the herb beauty oil according to any one of claims 1 to 6 in the preparation of cosmetics.

8. A repairing and nourishing essential oil, characterized in that: The invention comprises the following components in percentage by weight: 30-50% squalane, 10-30% hydrogenated polyisobutene, 10-20% caprylic / capric triglyceride, 1-2% isododecane, 0.1-1% tocopherol, 1-10% herb beauty oil according to any one of claims 1 to 6, and the balance grape seed oil.

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