Traditional Chinese medicine composition for repelling mosquitoes, relieving itching and relieving swelling and pain as well as preparation method and application of traditional Chinese medicine composition

Abstract

The invention relates to the technical field of traditional Chinese medicines, in particular to a traditional Chinese medicine composition for repelling mosquitoes, relieving itching and relieving swelling and pain as well as a preparation method and application thereof. The blumea balsamifera, the menthol, the fructus cinnamoii oil, the citionella oil and the alpinia zerumbet volatile oil are creatively matched to be used for repelling mosquitoes, relieving itching and relieving swelling and pain, and the five components supplement one another and can achieve a synergistic effect; the traditional Chinese medicine compound mosquito-repelling and itching-relieving preparation obtained by blending and combining has remarkable mosquito-repelling and itching-relieving effects and has the functions of relieving swelling and pain safely and without irritation, promoting recovery of inflammation parts and enhancing the skin repairing function.

Description

Technical Field

[0001] The invention relates to the technical field of traditional Chinese medicines, and in particular to a traditional Chinese medicine composition for repelling mosquitoes, relieving itching, reducing swelling and relieving pain, and a preparation method and application thereof. Background Art

[0002] Mosquitoes are carriers of pathogens of many mosquito-borne infectious diseases, such as malaria, filariasis, dengue fever, and epidemic encephalitis B. The skin bitten by mosquitoes will experience local itching, pain, redness and swelling. Severe scratching can cause infection, seriously interfering with people's normal life and work, and posing a threat to human health.

[0003] At present, most of the mosquito repellent products on the market contain chemical ingredients such as pyrethrin, DEET, and pyrethrins. Long-term use not only pollutes the environment, but also has certain effects on human health. For example, pyrethrin not only induces diseases such as asthma, but long-term use can also cause cancer and teratogenesis. DEET can inhibit the activity of acetylcholinesterase in the central nervous system of insects and mammals, and frequent use can easily irritate the skin. Pyrethrins have a greater impact on the human nervous system, and long-term use can cause symptoms such as headaches, dizziness, and abnormal nerve sensations.

[0004] my country is rich in plant resources, and there are thousands of medicinal plants for mosquito repellent. The development of plant-based mosquito repellent and antipruritic drugs has unique advantages, which meets people's demand for safe and natural plant-based mosquito repellents, which are natural and non-irritating. Therefore, the preparation of safe and effective mosquito repellent, antipruritic, anti-swelling and analgesic products has attracted much attention.

[0005] Therefore, the current key research direction is to find a mosquito repellent and antipruritic drug that has significant mosquito repellent and antipruritic effects, is safe and non-irritating, does not contain ingredients such as chlorpyrifos, DEET, cypermethrin and fragrances, and uses a natural aromatic smell to repel mosquitoes. Summary of the invention

[0006] In order to solve the above technical problems existing in the prior art, the present invention provides a Chinese medicine composition for repelling mosquitoes, relieving itching, reducing swelling and relieving pain, and its preparation method and application. Specifically, it is achieved through the following technical solutions:

[0007] A Chinese medicinal composition for repelling mosquitoes, relieving itching, reducing swelling and relieving pain comprises the following raw materials: moxa tablets, menthol, Litsea cubeba oil, citronella oil and Alpinia galanga volatile oil.

[0008] Furthermore, the weight proportions of the raw materials are: 0.1-20 parts of moxa slices, 0.1-20 parts of menthol, 0.05-6 parts of Litsea cubeba oil, 0.1-5 parts of citronella oil, and 0.05-2.5 parts of Alpinia galanga volatile oil.

[0009] Furthermore, suitable pharmaceutical excipients may be added to prepare suitable external preparations, including but not limited to sprays, ointments, creams or gels.

[0010] Furthermore, the pharmaceutical excipients include one or more of the following ingredients: yellow vaseline, beeswax, paraffin, glycerin, Tween 80, carbomer 940, triethanolamine, purified water, propylene glycol, and 95% ethanol.

[0011] The traditional Chinese medicine composition has good mosquito repelling, itching relieving, swelling and pain relieving effects, and has good application prospects in the preparation of mosquito repelling, itching relieving, swelling and pain relieving drugs.

[0012] Furthermore, the drug for repelling mosquitoes, relieving itching, reducing swelling and relieving pain is an external preparation, which includes but is not limited to sprays, ointments, creams or gels.

[0013] The compound mosquito repellent and antipruritic ointment prepared by using the traditional Chinese medicine composition comprises the following raw materials by weight percentage: 20% of moxa slices, 20% of menthol, 6% of Litsea cubeba oil, 5% of citronella oil, and 2.5% of Alpinia galanga volatile oil. The specific weight percentages of the ingredients are as follows:

[0014]

[0015] The specific preparation method comprises the following steps:

[0016] (1) Grind the moxa slices and menthol together, add Litsea cubeba oil, citronella oil, and Alpinia officinalis volatile oil and mix well;

[0017] (2) melting yellow vaseline, beeswax and paraffin at 80-90° C. and adding the product obtained in step (1);

[0018] (3) Pack and cool.

[0019] The obtained compound mosquito repellent and antipruritic ointment has the characteristics of being easy to use, simple in process, low in cost, safe and non-irritating. The yellow vaseline has a lubricating effect, so that the product has good coating properties. At the same time, the yellow vaseline is a commonly used skin moisturizer with a strong moisturizing effect. Applying it in an appropriate amount is equivalent to a protective film, which makes it difficult for the moisture on the skin surface to evaporate, and can prevent bacteria in the air from contacting the skin, thereby reducing the risk of infection. Therefore, it has the effects of protecting the skin, preventing infection, and preventing allergies.

[0020] The compound mosquito repellent and antipruritic cream prepared by using the traditional Chinese medicine composition comprises the following raw materials by weight percentage: 6% of moxa slices, 6% of menthol, 4% of Litsea cubeba oil, 2.5% of citronella oil, and 1% of Alpinia galanga volatile oil. The specific weight percentages of the ingredients are as follows:

[0021]

[0022] The specific preparation method comprises the following steps:

[0023] (1) Dissolve the moxa tablets and menthol in 95% ethanol;

[0024] (2) Mix the Litsea cubeba oil, citronella oil, and Alpinia galanga volatile oil, add Tween 80 and stir evenly;

[0025] (3) adding the product obtained in step (1) to the product obtained in step (2) and mixing them evenly;

[0026] (4) Carbomer 940 is swollen with water and then glycerol is added;

[0027] (5) slowly adding the product obtained in step (3) to the product obtained in step (4), stirring evenly, and then adding triethanolamine to adjust the pH to 6 to 6.5;

[0028] (6) Add the remaining amount to purified water and divide into portions.

[0029] The compound mosquito repellent and antipruritic spray prepared by using the traditional Chinese medicine composition comprises the following raw materials by weight percentage: 1.5% of moxa slices, 1% of menthol, 0.15% of Litsea cubeba oil, 0.1% of citronella oil, and 0.05% of Alpinia officinalis volatile oil. The specific weight percentages of the components are as follows:

[0030]

[0031] The specific preparation method comprises the following steps:

[0032] (1) Add 95% ethanol to the mugwort tablets, menthol, Litsea cubeba oil, citronella oil, and Alpinia officinalis volatile oil and stir to dissolve;

[0033] (2) adding propylene glycol to the product obtained in step (1) and stirring evenly;

[0034] (3) Make up the remainder with purified water and divide into smaller pieces.

[0035] The compound mosquito repellent and antipruritic gel prepared by using the Chinese medicine composition comprises the following raw materials by weight percentage: 0.1% of moxa slices, 0.1% of menthol, 0.25% of Litsea cubeba oil, 0.2% of citronella oil, and 0.1% of Alpinia galanga volatile oil. The specific weight percentages of the components are as follows:

[0036]

[0037] The specific preparation method comprises the following steps:

[0038] (1) Dissolve the moxa tablets and menthol in 95% ethanol;

[0039] (2) Mix the Litsea cubeba oil, citronella oil, and Alpinia galanga volatile oil, add Tween 80 and stir evenly;

[0040] (3) adding the product obtained in step (1) to the product obtained in step (2), mixing evenly, and adding the remaining amount of purified water in the prescription;

[0041] (4) Sprinkle carbomer on a suitable amount of water to swell, add glycerol and mix evenly, then add triethanolamine to adjust the pH to 6-6.5;

[0042] (5) Slowly add the product obtained in step (3) to the product obtained in step (4), stirring while adding, until the product is formed, and then divide it into portions.

[0043] Mugwort tablets: Mugwort tablets are crystals extracted from the fresh leaves of the Asteraceae plant Artemisia balsamina, which are included in the 2020 edition of the Chinese Pharmacopoeia. They are pungent and bitter, slightly cold in nature, and have the effects of refreshing the mind, clearing away heat and relieving pain. They are often used for fever, coma, stroke, phlegm, coma, red eyes, mouth sores, sore throat, etc. In the 2003 edition of the "Quality Standards for Chinese Medicinal Materials and Ethnic Medicinal Materials in Guizhou Province" and the 2008 edition of the "Jiangxi Province Traditional Chinese Medicine Preparation Specifications", mugwort tablets also have the effects of refreshing the mind, clearing heat and relieving pain. In addition, according to the 2020 edition of the "Inner Mongolia Mongolian Medicine Preparation Specifications", mugwort tablets have the effects of clearing heat, reducing swelling and relieving pain. And there are relevant literature reports that mugwort tablets can relieve the corresponding symptoms such as itching, pain, redness, swelling and inflammation caused by mosquito bites.

[0044] Menthol: Also known as menthol, it is a monoterpene organic compound that can be extracted from the stems and leaves of mint and is the main component of mint volatile oil. Modern pharmacological research shows that menthol has pharmacological effects such as anti-inflammatory and analgesic, antipruritic and detoxifying, and promoting transdermal absorption. It is widely used in medicine, daily necessities, and food. It can not only repel mosquitoes and insects, but also act on mucous membranes or skin, have a cooling and antipruritic effect, and can reduce the itching sensation after mosquito bites.

[0045] Litsea cubeba oil: This product is a volatile oil obtained by steam distillation of the dried fruit of the Lauraceae plant Migao. It is included in the 2019 edition of the Quality Standards for Traditional Chinese and Ethnic Medicinal Materials in Guizhou Province. It can be used externally to repel mosquitoes and flies. The main active ingredients of Litsea cubeba, such as eucalyptol, p-cymene, and α-terpineol, have anti-inflammatory, antibacterial, and antioxidant activities.

[0046] Citronella oil: Citronella oil, also known as vanilla oil or male stalk oil, is a volatile oil obtained by distilling the fresh stems and leaves of the genus Citronella. It is included in the 2003 edition of the Quality Standards for Traditional Chinese and Ethnic Chinese Medicines in Guizhou Province. It has the effects of dispelling wind and relieving exterior symptoms, warming the middle and relieving pain. In addition, literature reports that citronella oil is a natural mosquito repellent. Its main mosquito repellent ingredients are citronellal (acyclic monoterpene) and geraniol. It has the advantages of being natural and safe, and having a good mosquito repellent effect.

[0047] Alpinia galanga volatile oil: This product is a volatile oil obtained by steam distillation of the dried fruit of the ginger plant Alpinia galanga. Most ginger plants have aromatic odors and are common mosquito repellent plants. Literature reports that its volatile oil has significant anti-inflammatory and analgesic effects.

[0048] Mugwort tablets have the effects of refreshing the mind, clearing away heat and relieving pain; menthol has the effects of clearing wind and dispersing heat, and is mainly used for wind-heat colds, headaches, sore throats, and skin itching. For the heat and toxicity accumulation syndrome caused by epidermatitis in traditional Chinese medicine, the combination of the two can effectively relieve symptoms such as local itching, dryness, tingling, and redness and swelling of the skin. In addition, the oil of Litsea cubeba has a fragrant smell and a spicy taste. Modern pharmacological studies have shown that it has insect repellent, antibacterial, and antioxidant activities; citronella oil is spicy and warm in nature, and has the effects of clearing heat and detoxifying, dispelling wind and relieving pain. Alpinia officinalis volatile oil has significant anti-inflammatory and analgesic effects. According to traditional Chinese medicine, the treatment principles of skin inflammation caused by mosquito bites are clearing heat and purging fire, detoxifying and reducing swelling, promoting blood circulation and cooling blood, and dispelling wind and relieving itching. These natural plant essential oils are reasonably matched. In addition, mugwort tablets and menthol are used together, and the material effects complement each other, which can further enhance the efficacy and achieve the effects of insect repellent, anti-swelling and itching, calming and soothing, and antibacterial and moisturizing.

[0049] Compared with the prior art, the technical effects created by the present invention are embodied in:

[0050] (1) The present invention creatively combines moxa slices, menthol, Litsea cubeba oil, citronella oil and Alpinia officinalis volatile oil for mosquito repellent, itching, swelling and pain relief. The five ingredients complement each other and can synergize. Through the synergistic effect between the components, the Chinese medicine compound mosquito repellent and itching relief preparation obtained by the combination has significant mosquito repellent and itching relief effect, safe and non-irritating swelling and pain relief, promotes the recovery of the inflammation site, and enhances the skin repair function. The final product is significantly better than other products that do not use the five ingredients in combination.

[0051] (2) The Chinese herbal compound mosquito repellent and antipruritic preparation of the present invention does not contain ingredients such as chlorpyrifos, DEET, cypermethrin and essence, and uses natural aromatic odor to repel mosquitoes. Insects are not likely to develop drug resistance thereto, and the preparation is safe, environmentally friendly and non-toxic. DETAILED DESCRIPTION

[0052] The technical solution of the present invention is further defined below in conjunction with specific implementation methods, but the scope of protection required is not limited to the description.

[0053] Example 1 A Chinese herbal compound mosquito repellent and itching-relieving stick ointment

[0054] The Chinese medicinal compound mosquito repellent and itching-relieving stick-shaped ointment comprises the following components in percentage by weight:

[0055]

[0056] The preparation method of the above-mentioned Chinese medicine compound mosquito repellent and itching-relieving stick-shaped ointment mainly comprises the following steps:

[0057] (1) Grind the moxa slices and menthol, add Litsea cubeba oil, citronella oil, and Alpinia officinalis volatile oil, and stir evenly;

[0058] (2) melting yellow vaseline, beeswax and paraffin at 80-90° C. and adding the product obtained in step (1);

[0059] (3) Pack and cool.

[0060] Embodiment 2 A kind of Chinese medicine compound mosquito repellent and itching cream

[0061] The Chinese medicinal compound mosquito repellent and antipruritic cream comprises the following components in percentage by weight:

[0062]

[0063] The preparation method of the above-mentioned Chinese medicine compound mosquito repellent and antipruritic cream mainly comprises the following steps:

[0064] (1) Dissolve the moxa tablets and menthol in 95% ethanol;

[0065] (2) Mix the Litsea cubeba oil, citronella oil, and Alpinia officinalis essential oil evenly, add Tween 80 and stir evenly.

[0066] (3) Add the product obtained in step (1) to the product obtained in step (2) and mix well

[0067] (4) Carbomer 940 is swollen with water and then glycerol is added

[0068] (5) Slowly add the product obtained in (3) to the product obtained in (4), stir evenly, then add triethanolamine and adjust the pH to 6-6.5.

[0069] (6) Add the remaining amount to purified water and divide into smaller pieces.

[0070] Embodiment 3 A Chinese medicine compound mosquito repellent and itching spray

[0071] The Chinese medicine compound mosquito repellent and antipruritic spray comprises the following components in percentage by weight:

[0072]

[0073] The preparation method of the above-mentioned Chinese medicine compound mosquito repellent and antipruritic spray mainly comprises the following steps:

[0074] (1) Add 95% ethanol to dissolve the moxa slices, menthol, Litsea cubeba oil, citronella oil and Alpinia officinalis volatile oil.

[0075] (2) Add propylene glycol to the product obtained in step (1) and stir evenly

[0076] (3) Make up the balance with purified water. Then dispense the mixture.

[0077] Embodiment 4 A kind of Chinese medicine compound mosquito repellent and itching relieving gel

[0078] The Chinese medicinal compound mosquito repellent and antipruritic gel comprises the following components in percentage by weight:

[0079]

[0080] The preparation method of the above-mentioned Chinese medicine compound mosquito repellent and antipruritic gel mainly comprises the following steps:

[0081] (1) Dissolve the moxa tablets and menthol in 95% ethanol;

[0082] (2) Mix the Litsea cubeba oil, citronella oil and Alpinia galanga volatile oil, add Tween 80 and stir evenly;

[0083] (3) adding the product obtained in step (1) to the product obtained in step (2), mixing evenly, and adding the remaining amount of purified water in the prescription;

[0084] (4) Sprinkle carbomer on a suitable amount of water to swell, add glycerol and mix evenly, then add triethanolamine to adjust the pH to 6-6.5;

[0085] (5) Slowly add the product obtained in step (3) to the product obtained in step (4), stirring while adding until it is formed, and then divide it into packages to obtain

[0086] Comparative Example 1: The preparation method thereof is the same as that of Example 1 except that 20% of moxa tablets, 20% of menthol, 6% of Litsea cubeba oil, 5% of citronella oil and 2.5% of Alpinia galanga volatile oil in the raw materials are replaced with 31.9% of menthol, 9.6% of Litsea cubeba oil, 8.0% of citronella oil and 4% of Alpinia galanga volatile oil.

[0087] Comparative Example 2: The preparation method is the same as that in Example 1, the raw materials of 20% of mugwort tablets, 20% of menthol, 6% of Litsea cubeba oil, 5% of citronella oil, and 2.5% of Alpinia galanga volatile oil are replaced with 31.9% of mugwort tablets, 9.6% of Litsea cubeba oil, 8.0% of citronella oil, and 4% of Alpinia galanga volatile oil.

[0088] Comparative Example 3: The preparation method is the same as that of Example 1 except that 20% of mugwort tablets, 20% of menthol, 6% of Litsea cubeba oil, 5% of citronella oil, and 2.5% of Alpinia galanga volatile oil in the raw materials are replaced with 22.5% of mugwort tablets, 22.5% of menthol, 5.7% of citronella oil, and 2.8% of Alpinia galanga volatile oil.

[0089] Comparative Example 4: The preparation method is the same as that in Example 1, the raw materials are replaced with 22.1% of mugwort tablets, 22.1% of menthol, 6% of Litsea cubeba oil, 5% of citronella oil, and 2.5% of Alpinia galanga volatile oil. The rest are the same as in Example 1.

[0090] Comparative Example 5: The preparation method is the same as that of Example 1 except that 20% of mugwort tablets, 20% of menthol, 6% of Litsea cubeba oil, 5% of citronella oil, and 2.5% of Alpinia galanga volatile oil in the raw materials are replaced with 21.0% of mugwort tablets, 21.0% of menthol, 6.3% of Litsea cubeba oil, and 5.2% of citronella oil.

[0091] Comparative Example 6: The preparation method is the same as that of Example 1 except that 20% of mugwort tablets, 20% of menthol, 6% of Litsea cubeba oil, 5% of citronella oil and 2.5% of Alpinia galanga volatile oil in the raw materials are replaced with 53.5% of mugwort tablets.

[0092] Comparative Example 7: The preparation method thereof is the same as that of Example 1 except that 20% of moxa tablets, 20% of menthol, 6% of Litsea cubeba oil, 5% of citronella oil and 2.5% of Alpinia galanga volatile oil in the raw materials are replaced with 53.5% of menthol, and the rest are the same as those of Example 1.

[0093] Comparative Example 8: The preparation method thereof is the same as that of Example 1 except that 20% of moxa tablets, 20% of menthol, 6% of Litsea cubeba oil, 5% of citronella oil and 2.5% of Alpinia galanga volatile oil in the raw materials are replaced with 53.5% of Litsea cubeba oil, and the rest are the same as those of Example 1.

[0094] Comparative Example 9: The preparation method thereof is the same as that of Example 1 except that 20% of moxa tablets, 20% of menthol, 6% of Litsea cubeba oil, 5% of citronella oil and 2.5% of Alpinia galanga volatile oil in the raw materials are replaced with 53.5% of citronella oil. The rest are the same as those of Example 1.

[0095] Comparative Example 10: The preparation method thereof is the same as that of Example 1 except that 20% of moxa tablets, 20% of menthol, 6% of Litsea cubeba oil, 5% of citronella oil and 2.5% of Alpinia galanga volatile oil in the raw materials are replaced with 53.5% of Alpinia galanga volatile oil, and the rest are the same as those of Example 1.

[0096] Test Example 1: Antipruritic effect test

[0097] 1. Test materials: mosquito repellent and antipruritic preparations prepared in Examples 1-4, preparations of Comparative Examples 1-10, histamine phosphate (Shanghai Tengzhun Biotechnology Co., Ltd., analytical grade, batch number: DLX441), compound dexamethasone acetate ointment (positive control drug, China Resources Sanjiu Pharmaceutical Co., Ltd., batch number H44024170).

[0098] 2. Experimental subjects: Kunming SPF mice, weighing 18-22g, half male and half female.

[0099] 3. Test method: 96 mice, half male and half female, were randomly divided into 16 groups. The blank control group (normal saline) had 6 mice; the drug administration group (mosquito repellent and antipruritic preparations prepared in Examples 1-4, preparations in Comparative Examples 1-10) had 6 mice each; the positive group (compound dexamethasone acetate ointment) had 6 mice. The back of the right hind foot of each group of mice was shaved, with an area of ​​1 cm×1 cm. The shaved part of the drug administration group and the positive drug group was coated with the mosquito repellent and antipruritic preparations prepared in Example 1-4, the preparations of Comparative Examples 1-10 and the compound dexamethasone acetate ointment, respectively, once every 15 minutes for 6 consecutive times, and then the shaved part of the back of the right hind foot was scratched with coarse sandpaper until bleeding, and then the drug administration group was coated with the mosquito repellent and antipruritic preparations prepared in Example 1-4 and the preparations of Comparative Examples 1-10, and the positive group was coated with the compound dexamethasone acetate ointment once, respectively. 10 minutes after the last coating, 0.01% histamine phosphate was dripped on the wound surface, 10 μL for each, and thereafter, 10 μL was dripped every 3 minutes according to the increasing concentration of 0.02%, 0.03%, 0.04%..., until the mouse turned back and licked the right hind foot, and the cumulative amount of histamine phosphate administered when the mouse finally turned back and licked the right hind foot was taken as the itch threshold, and the itch threshold of each group of animals was recorded and compared.

[0100] Table 1 Effects of Examples and Comparative Examples on the Itch Threshold of Histamine Phosphate-induced Mice ( n=6)

[0101]

[0102] Note: Compared with the blank control group, ### P<0.001; compared with Example 1, * P<0.05, ** P<0.01

[0103] The preparation raw materials of Comparative Examples 1-5 are respectively lacking mugwort tablets, menthol, Litsea cubeba oil, citronella oil and Alpinia galanga volatile oil. Compared with Example 1, the itch threshold of Comparative Examples 1-5 groups is significantly reduced, indicating that the lack of any of the five ingredients of mugwort tablets, menthol, Litsea cubeba oil, citronella oil and Alpinia galanga volatile oil will weaken the antipruritic effect.

[0104] The preparation raw materials of Comparative Examples 6-10 contain only one of the five components of mugwort tablets, menthol, Litsea cubeba oil, citronella oil and Alpinia galanga volatile oil. Compared with Example 1, the itch threshold is significantly reduced, indicating that only one of the five components of mugwort tablets, menthol, Litsea cubeba oil, citronella oil and Alpinia galanga volatile oil cannot fully exert the antipruritic effect, and the five components must be used in combination to synergistically enhance the effect and exert the best antipruritic effect.

[0105] Compared with the blank group, the positive control group and the groups of Examples 1-4 increased the itch threshold of mice caused by histamine phosphate, and the difference was statistically significant (P was less than 0.001). The results showed that the mosquito repellent and antipruritic preparations prepared in Examples 1-4 could significantly increase the itch threshold of histamine phosphate on mice.

[0106] Test Example 2: Detumescence Effect Test

[0107] 1. Test materials: mosquito repellent and antipruritic preparations prepared in Examples 1-4, preparations of Comparative Examples 1-10, o-xylene (analytical grade, Tianjin Komiou Chemical Reagent Co., Ltd.), compound dexamethasone acetate ointment (positive control drug, China Resources Sanjiu Pharmaceutical Co., Ltd., batch number H44024170).

[0108] 2. Experimental subjects: Kunming SPF mice, weighing 18-22g, half male and half female.

[0109] 3. Test method: 96 mice were selected, half male and half female, and randomly divided into 16 groups, 6 in each group. They were respectively a blank control group (normal saline), a drug administration group (mosquito repellent and antipruritic preparations obtained in Examples 1-4, and preparations of Comparative Examples 1-10), and a positive group (compound dexamethasone acetate ointment). The right ear of each group of mice was administered once every 15 minutes, for a total of 6 times, and the left ear was not treated. After the last administration for 15 minutes, the right ear of the mouse was washed with pure water, wiped dry with cotton balls, and both sides of the left ear were coated with 20 μL of xylene to cause inflammation. After 30 minutes, the neck was dislocated and killed, and the ears were cut off along the auricle baseline. A round ear piece was punched in the same position of the ears with a puncher with a diameter of 8 mm, and weighed separately. The left ear was used as a control, and the difference in the mass of the left and right ear pieces was the degree of swelling. The swelling degree of the ear pieces of each group of mice and the ear swelling inhibition rate were calculated. The average value of ear swelling in each group of mice was calculated, and statistical analysis was performed.

[0110] Swelling degree (mg) = right ear auricle mass - left ear auricle mass

[0111] Swelling inhibition rate (%) = (mean swelling of the right ear auricle in the model group - mean swelling of the right ear auricle in the drug-treated group) / mean swelling of the right ear auricle in the model group × 100%

[0112] Table 2 Effects of Examples and Comparative Examples on xylene-induced ear swelling in mice ( n=6)

[0113]

[0114] Note: Compared with the blank control group, ### P<0.001; compared with Example 1, * P<0.05, ** P<0.01

[0115] The preparation raw materials of Comparative Examples 1-5 lacked mugwort tablets, menthol, Litsea cubeba oil, citronella oil and Alpinia galanga volatile oil, respectively. Compared with Example 1, the differences in ear swelling between the mice in Comparative Examples 1-5 groups were statistically significant (all P less than 0.05), indicating that the absence of any of the five components of mugwort tablets, menthol, Litsea cubeba oil, citronella oil and Alpinia galanga volatile oil would weaken the detumescent effect.

[0116] The preparation raw materials of Comparative Examples 6-10 only contain one of the five components of mugwort tablets, menthol, Litsea cubeba oil, citronella oil and Alpinia galanga volatile oil. Compared with Example 1, the difference in the swelling degree of the mouse auricle is statistically significant (P is less than 0.05), indicating that only one of the five components of mugwort tablets, menthol, Litsea cubeba oil, citronella oil and Alpinia galanga volatile oil cannot fully exert the detumescent effect, and the five components must be used in combination to synergize and exert the best detumescent effect.

[0117] Compared with the blank control group, the positive control group and Example 1-4 groups all showed statistically significant differences in the swelling of the mouse auricle (all P less than 0.001); the results showed that the mosquito repellent and antipruritic preparations prepared in Examples 1-4 could significantly inhibit xylene-induced inflammation of the mouse auricle.

[0118] Test Example 3: Analgesia Test

[0119] 1. Experimental materials: mosquito repellent and antipruritic preparations prepared in Examples 1-4, preparations of Comparative Examples 1-10, glacial acetic acid (Chongqing Chuandong Chemical (Group) Co., Ltd., batch number 20191001); Voltaren latex cream (GlaxoSmithKline, UK, batch number FK2C-C).

[0120] 2. Experimental subjects: Kunming SPF mice, weighing 18-22g, half male and half female.

[0121] 3. Experimental method: 96 mice, half male and half female, were randomly divided into 16 groups, 6 mice in each group. They were respectively a blank control group (normal saline), a drug-treated group (mosquito repellent and antipruritic preparations prepared in Examples 1-4, preparations in Comparative Examples 1-10), and a positive group (Voltaren latex cream). The medicine was applied once a day (depilatory cream was used to remove hair one day before administration) for 5 consecutive days. One hour after the last administration, 1% acetic acid solution was injected intraperitoneally, 10 mL / kg, to induce a writhing reaction in mice. The time was recorded immediately after the injection, and the number of writhing times of mice in each group within 20 minutes was observed, with the concavity of the mouse abdomen, extension of the hind limbs, and elevation of the buttocks as indicators. The analgesic rate of each drug-treated group was calculated based on the number of writhing times.

[0122]

[0123] Table 3 Effects of Examples and Comparative Examples on Analgesia Rate in Mice ( n=6)

[0124]

[0125] Note: Compared with the blank control group, ### P<0.001; compared with Example 1, * P<0.05

[0126] The preparation raw materials of Comparative Examples 1-5 lacked mugwort tablets, menthol, Litsea cubeba oil, citronella oil and Alpinia galanga volatile oil, respectively. Compared with Example 1, the analgesic effects of Comparative Examples 1-5 were weakened, and the differences were statistically significant (P was less than 0.05), indicating that the absence of any of the five components of mugwort tablets, menthol, Litsea cubeba oil, citronella oil and Alpinia galanga volatile oil would weaken the analgesic effect.

[0127] The preparation raw materials of Comparative Examples 6-10 contain only one of the five components of mugwort tablets, menthol, Litsea cubeba oil, citronella oil and Alpinia galanga volatile oil. Compared with Example 1, the analgesic effect is significantly weakened, indicating that only one of the five components of mugwort tablets, menthol, Litsea cubeba oil, citronella oil and Alpinia galanga volatile oil cannot fully exert the mosquito repellent effect, and the five components must be used in combination to synergistically enhance the effect and exert the best analgesic effect.

[0128] As shown in Table 3, both the positive control group and the groups of Examples 1-4 had significant analgesic effects on mice, and the results were statistically significant (P was less than 0.001); the results showed that the mosquito repellent and antipruritic preparations prepared in Examples 1-4 could significantly relieve the pain of mice.

[0129] Test Example 4: On-site mosquito repellent effect test

[0130] Test materials: mosquito repellent and antipruritic preparations prepared in Examples 1-4 and preparations in Comparative Examples 1-10

[0131] 2. Experimental subjects: 210 volunteers aged 20-50 years old

[0132] 3. Test method: 210 volunteers were randomly divided into 14 groups, each with 15 people. Each group was sprayed or smeared evenly on the bare arms with the mosquito repellent and antipruritic preparations prepared in Examples 1-4 and the preparations of Comparative Examples 1-10, and tested in waterside, woods and other places with many mosquitoes. After 3 hours, the volunteers were evaluated according to their own test results. The results are shown in Table 4:

[0133] Table 4 Experimental results of mosquito repellent of the embodiment and the contrast agent

[0134]

[0135] The preparation raw materials of Comparative Examples 1-5 are respectively lacking mugwort tablets, menthol, Litsea cubeba oil, citronella oil and Alpinia galanga volatile oil. Compared with Example 1, the mosquito repellent effect of Comparative Examples 1-5 is weakened, indicating that the lack of any of the five ingredients of mugwort tablets, menthol, Litsea cubeba oil, citronella oil and Alpinia galanga volatile oil will weaken the mosquito repellent effect.

[0136] The preparation raw materials of Comparative Examples 6-10 only contain one of the five components of mugwort tablets, menthol, Litsea cubeba oil, citronella oil and Alpinia galanga volatile oil. Compared with Example 1, the mosquito repellent effect is weakened, indicating that only one of the five components of mugwort tablets, menthol, Litsea cubeba oil, citronella oil and Alpinia galanga volatile oil cannot fully exert the mosquito repellent effect, and the five components must be used in combination to synergistically enhance the effect and exert the best mosquito repellent effect.

[0137] As shown in Table 6, Examples 1-4 all have mosquito repellent effects. In the experiment, mosquitoes can be seen flying around the body, but they do not bite the exposed arms. This shows that the mosquito repellent and antipruritic preparations provided by Examples 1-4 of the present invention have effective mosquito repellent and anti-bite effects.

[0138] Based on the above experimental results, it can be seen that the five ingredients in the prescription complement each other and can synergistically enhance their effects to achieve the best mosquito repellent, antipruritic, anti-swelling and analgesic effects.

[0139] Finally, it should be pointed out that the above embodiments are only representative examples of the present invention. Obviously, the technical solution of the present invention is not limited to the above embodiments, and there are many variations. All variations that can be directly derived or associated with the content disclosed by ordinary technicians in this field should be considered as the protection scope of the present invention.

Claims

1. A Chinese medicine composition for repelling mosquitoes, relieving itching, reducing swelling and relieving pain, characterized in that: The invention comprises the following raw materials: mugwort tablets, menthol, Litsea cubeba oil, citronella oil and Alpinia galanga volatile oil.

2. The Chinese medicine composition according to claim 1, characterized in that The weight proportions of the raw materials are as follows: 0.1-20 parts of moxa slices, 0.1-20 parts of menthol, 0.05-6 parts of Litsea cubeba oil, 0.1-5 parts of citronella oil, and 0.05-2.5 parts of Alpinia galanga volatile oil.

3. The Chinese medicine composition according to claim 1, characterized in that It also contains pharmaceutical excipients.

4. The Chinese medicine composition according to claim 3, characterized in that: The pharmaceutical excipients include one or more of the following ingredients: yellow vaseline, beeswax, paraffin, olive oil, glycerin, Tween 80, carbomer 940, triethanolamine, purified water, propylene glycol, and 95% ethanol.

5. Use of the Chinese medicine composition according to claim 1 in preparing drugs for repelling mosquitoes, relieving itching, reducing swelling and relieving pain.

6. The use according to claim 5, characterized in that: The drug for repelling mosquitoes, relieving itching, reducing swelling and relieving pain is an external preparation, which is a spray, an ointment, a cream or a gel.

7. A compound mosquito repellent and antipruritic ointment prepared using the Chinese medicine composition according to claim 1, characterized in that: The raw materials are calculated by weight percentage: 20% of moxa slices, 20% of menthol, 6% of Litsea cubeba oil, 5% of citronella oil and 2.5% of Alpinia galanga volatile oil.

8. A compound mosquito repellent and antipruritic ointment prepared using the Chinese medicine composition according to claim 1, characterized in that: The raw materials are calculated by weight percentage: 6% of moxa slices, 6% of menthol, 4% of Litsea cubeba oil, 2.5% of citronella oil, and 1% of Alpinia galanga volatile oil.

9. The compound mosquito repellent and antipruritic spray prepared using the Chinese medicine composition according to claim 1, characterized in that: The raw materials are calculated by weight percentage: 1.5% of moxa slices, 1% of menthol, 0.15% of Litsea cubeba oil, 0.1% of citronella oil, and 0.05% of Alpinia officinalis volatile oil.

10. The compound mosquito repellent and antipruritic gel prepared using the Chinese medicine composition according to claim 1, characterized in that: The raw materials are calculated by weight percentage as follows: 0.1% of moxa slices, 0.1% of menthol, 0.25% of Litsea cubeba oil, 0.2% of citronella oil, and 0.1% of Alpinia galanga volatile oil.

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