In-situ straw particle bioreactor containing wolfberry waste

Abstract

The invention discloses an in-situ straw particle bioreactor containing Chinese wolfberry fruit waste. The method comprises the following steps: S1, digging grooves in an overwintering idle greenhouse; s2, filling the dug groove with straw and waste; s3, then uniformly spreading strains; s4, soil lifting and backfilling; s5, watering and planting seedlings; s6, punching in time after field planting; according to the method, the concentration of carbon dioxide is increased due to the fact that strains are applied to the wolfberry branches and the crop straw, and the straw waste bioreactor can release carbon dioxide and also can release a large amount of heat; biological pest control; some germs are killed, the pesticide consumption is low, the pesticide residue is low, the pesticide is saved, the harvesting time is prolonged, and the effects of increasing the yield and reducing the pesticide are achieved; after the straws and the Chinese wolfberry wastes are decomposed, organic matters are left in the soil, so that the soil is fertile but not loose, root systems of crops are better bound, and the Chinese wolfberry wastes also contain a large amount of mineral elements, so that the soil is well improved.

Description

Technical Field

[0001] The invention belongs to the technical field of particle bioreactors, and in particular relates to an in-situ straw particle bioreactor containing wolfberry waste. Background Art

[0002] The straw bioreactor technology system is a new concept of organic cultivation theory and technology for increasing agricultural production, quality and efficiency. It is fundamentally different from traditional agricultural technology. Its successful research has fundamentally gotten rid of the situation where agricultural production relies on chemical fertilizers. The straw bioreactor technology replaces chemical fertilizers with straw and pesticides with plant vaccines. It is closely integrated with rural realities, promotes the recycling and value-added utilization of resources and the effective transformation of multiple production factors, and combines ecological improvement and environmental protection with high-yield, high-quality and pollution-free production of crops. It provides scientific and technological support for increasing agricultural efficiency, increasing farmers' income, food safety and sustainable agricultural development, and opens up new paths. However, various straw reactors on the market still have various problems.

[0003] For example, an in-situ straw pellet bioreactor disclosed in the authorization announcement number CN107573119A realizes the crushing of crop straw and pressing it into pellets, and in a winter-warm greenhouse or greenhouse, digging furrows to apply it between crop rows or under ridges, and by adding bacteria and nitrogen sources, covering with soil, watering, punching and other operations, the straw is converted into carbon dioxide, heat, organic matter, etc. through aerobic fermentation, which is used for the growth and development of crops such as vegetables and fruit trees cultivated in facilities. However, it does not solve the problems that the existing straw reactors cannot produce carbon dioxide, improve plant photosynthesis, are not convenient for breeding beneficial bacteria, cannot achieve biological control of pests and diseases, and cannot provide rich mineral elements. For this reason, we propose an in-situ straw pellet bioreactor containing wolfberry waste. Summary of the invention

[0004] The object of the present invention is to provide an in-situ straw particle bioreactor containing wolfberry waste to solve the problems raised in the above background technology.

[0005] To achieve the above-mentioned purpose, the present invention provides the following technical scheme: an in-situ straw particle bioreactor containing wolfberry waste, comprising making a groove in an idle shed during winter, and stacking wolfberry waste branches or weeds from plant seedlings in the field inside the groove, and then placing corn straw on the upper part of the wolfberry waste branches or weeds from plant seedlings in the field, and sprinkling urea and bacteria, and finally backfilling the soil, watering, covering the film and planting seedlings, that is, increasing the concentration of carbon dioxide through wolfberry waste and bacteria, under the same light, the photosynthetic efficiency is improved, and the soil layer temperature can be increased, and the shed temperature can also be increased, when the straw and waste are decomposed by the bacteria, a large number of disease-resistant organisms and their offspring can be bred, effectively inhibiting the growth of pathogens, and the decomposed straw and waste can provide organic matter.

[0006] Preferably, data is collected in the trench by a collection sensor, including temperature collection, humidity collection, carbon dioxide collection and light collection. The temperature collection includes soil layer temperature collection and ambient temperature collection. The collection sensor includes a filter. The filter adopts a second-order IIR low-pass filter in the IIR filter. The calculation formula of the second-order IIR low-pass filter is as follows:

[0007]

[0008] y(n) is the output data, x n is the input data, a and b are the coefficients of the filter, n is the number of filtering times, k=0 is the data information processed this time, k=1 is the data information processed next time, and nk is the position of the data information.

[0009] A method for an in-situ straw particle bioreactor containing wolfberry waste comprises the following steps:

[0010] S1. Digging grooves in the unused greenhouses during the winter: digging grooves in the unused greenhouses during the winter, and the width of the grooves is 60-80 cm, and the depth of the grooves is 25-50 cm;

[0011] S2, filling the trench with straw and waste: filling the bottom of the trench with a layer of discarded wolfberry branches or weeds from plant seedlings in the field, and then laying a layer of corn straw on the upper part of the discarded wolfberry branches or weeds from plant seedlings in the field;

[0012] S3, then evenly spread the fungus: spread the straw and wolfberry waste evenly and compactly, then take out the cultured fungus or directly purchase the ready-made fungus and evenly spread it on the discarded wolfberry branches or the plant seedlings in the field, and the upper part of the corn stalks;

[0013] S4, backfilling: backfilling the soil taken out from the trench and evenly covering the corn stalks;

[0014] S5, watering and planting seedlings: after soil backfilling, watering is performed, and both ends of the corn stalks are also watered, and the abandoned branches of wolfberry or the fallen seedlings of plants in the field and the corn stalks are evenly watered, and then the ridges are leveled, covered with film and the seedlings are planted after a few days;

[0015] S6. Drill holes in time after transplanting: Drill holes obliquely from the outside to the inside in the row of transplanted seedlings, and the hole depth should reach the corn stalk layer.

[0016] Preferably, the trenches in S1 are dug along the planting rows, and the length of the trenches is the same as the row length of the planting field. When digging the trenches, the excavated soil is placed on both sides of the trenches to facilitate subsequent backfilling of the trenches and covering the discarded wolfberry branches or fallen plant seedlings and corn stalks in the field.

[0017] Preferably, abandoned wolfberry branches or weeds from plant seedlings in the field are laid at the bottom of the groove dug in S2, and then corn stalks are laid on the abandoned wolfberry branches or weeds from plant seedlings in the field, and the abandoned wolfberry branches or weeds from plant seedlings in the field and corn stalks are laid in multiple layers, and the thickness of each layer is 5 cm, the thickness of the abandoned wolfberry branches or weeds from plant seedlings in the field is 15-25 cm, and the thickness of the corn stalks is 10-15 cm, and after laying each layer of abandoned wolfberry branches or weeds from plant seedlings in the field and corn stalks in multiple layers, each layer is laid evenly and compacted.

[0018] Preferably, the cultured bacteria in S3 are activated by refrigerated bacteria, that is, the stored bacteria are placed in a suitable culture medium for culture, and the culture is expanded step by step to obtain a pure and strong culture, that is, a culture with vigorous activity and sufficient inoculation quantity;

[0019] First, remove the cryopreservation tube from the cryopreservation condition;

[0020] Then place the cryopreservation tube on a small test tube rack in a 37°C bath, i.e., a 37°C, 70% alcohol bath, in which a small test tube rack is placed in advance, and the liquid level should not reach the tube stopper;

[0021] Then, shake the cryopreservation tube gently continuously, and the liquid level should not reach the tube stopper until the ice is completely dissolved to activate the bacteria.

[0022] Finally, sterile culture is carried out and the culture is expanded step by step to obtain a pure and strong culture.

[0023] Preferably, the backfill thickness in S4 is 10 cm, which can not only completely cover the abandoned branches of wolfberry or the weeds and corn stalks of plant seedlings in the field, but also facilitate uniform watering. When backfilling, the straw must be exposed at both ends of the trench by 5-8 cm to facilitate watering and ventilation.

[0024] Preferably, the amount of discarded wolfberry branches or plant seedlings and weeds in the field in S2 is 2000-3000 kg per mu, the amount of corn stalks in the same proportion is 1300-1900 kg, and the amount of fungus per mu is 8-10 kg.

[0025] Preferably, the ridge leveling in S5 is to fill the upper surface of the groove with soil again after watering, and fill the depth of the settlement so that the upper surface of the groove can remain flush, and then cover the upper part of the groove with a film, and fix the edge of the film with a soil layer, and finally plant the seedlings to determine the planting, and select neat, healthy, virus-free and well-growing seedlings for planting.

[0026] Preferably, the punching in S6 is performed according to the determined distance and row of the planted seedlings, and the punching depth reaches the straw layer, and about 14 round steel bars are used to punch holes obliquely from the outside to the inside in the planting row to achieve planting of the seedlings.

[0027] Compared with the prior art, the present invention has the following beneficial effects:

[0028] The wolfberry branches and crop straws of the present invention increase the concentration of carbon dioxide by applying bacterial strains and the temperature of the facilities, and the photosynthetic efficiency is improved under the same light, thus solving the problem that the yield of greenhouse fruits and vegetables cannot be increased due to lack of carbon dioxide.

[0029] The straw waste bioreactor can not only release carbon dioxide, but also release a lot of heat, which can increase the ground temperature by 4-6℃, and of course the greenhouse temperature can also increase by 2-4℃;

[0030] Biological control of pests and diseases; the special strains contain a variety of beneficial organisms, which can also breed a large number of disease-resistant organisms and their offspring when decomposing straw and waste. The microorganisms distributed in the soil and on the leaves of plants can effectively inhibit the growth of pathogens and kill some pathogens, with less use of drugs and less pesticide residues, thus saving drugs and extending the harvest period, so as to achieve the effect of increasing production and reducing drugs;

[0031] After the straw and wolfberry waste are decomposed, there are still some residues. The residues contain a lot of organic matter. The organic matter remains in the soil, making the soil fertile but not loose, so that the roots of crops can be better taken root. The wolfberry waste also contains a lot of mineral elements, which can improve the soil well, making the fruits rich in nutrition and better tasting. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 It is a schematic diagram of the steps of the present invention. DETAILED DESCRIPTION

[0033] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0034] See also Figure 1 The present invention provides a technical solution: an in-situ straw particle bioreactor containing wolfberry waste, comprising making a groove in an idle shed overwintering, and stacking wolfberry waste branches or plant seedling weeds in the field inside the groove, and then placing corn straw on the upper part of the wolfberry waste branches or plant seedling weeds in the field, and sprinkling urea and bacteria, and finally backfilling the soil, watering, covering the film and planting seedlings, that is, the wolfberry waste and bacteria increase the concentration of carbon dioxide, under the same light, the photosynthetic efficiency is improved, and the soil layer temperature can be increased, and the shed temperature can also be increased, when the straw and waste are decomposed by the bacteria, a large number of disease-resistant organisms and their offspring can be bred, effectively inhibiting the growth of pathogens, and the decomposed straw and waste can provide organic matter.

[0035] In order to collect and process the soil and environmental data in the greenhouse and reduce the noise of the collected data information, in this embodiment, preferably, the groove is used to collect data through a collection sensor, including temperature collection, humidity collection, carbon dioxide collection and light collection. The temperature collection includes soil layer temperature collection and environmental temperature collection. The collection sensor includes a filter. The filter adopts a second-order IIR low-pass filter in the IIR filter. The calculation formula of the second-order IIR low-pass filter is as follows:

[0036]

[0037] y(n) is the output data, x n is the input data, a and b are the coefficients of the filter, n is the number of filtering times, k=0 is the data information processed this time, k=1 is the data information processed next time, and nk is the position of the data information.

[0038] A method for an in-situ straw particle bioreactor containing wolfberry waste comprises the following steps:

[0039] S1. Digging grooves in the unused greenhouses during the winter: digging grooves in the unused greenhouses during the winter, and the width of the grooves is 60-80 cm, and the depth of the grooves is 25-50 cm;

[0040] S2, filling the trench with straw and waste: filling the bottom of the trench with a layer of discarded wolfberry branches or weeds from plant seedlings in the field, and then laying a layer of corn straw on the upper part of the discarded wolfberry branches or weeds from plant seedlings in the field;

[0041] S3, then evenly spread the fungus: spread the straw and wolfberry waste evenly and compactly, then take out the cultured fungus or directly purchase the ready-made fungus and evenly spread it on the discarded wolfberry branches or the plant seedlings in the field, and the upper part of the corn stalks;

[0042] S4, backfilling: backfilling the soil taken out from the trench and evenly covering the corn stalks;

[0043] S5, watering and planting seedlings: after soil backfilling, watering is performed, and both ends of the corn stalks are also watered, and the abandoned branches of wolfberry or the fallen seedlings of plants in the field and the corn stalks are evenly watered, and then the ridges are leveled, covered with film and the seedlings are planted after a few days;

[0044] S6. Drill holes in time after transplanting: Drill holes obliquely from the outside to the inside in the row of transplanted seedlings, and the hole depth should reach the corn stalk layer.

[0045] In order to realize the composting treatment of the corresponding reactor on the planting site, in this embodiment, preferably, the trenches in S1 are dug along the planting rows, and the length of the trenches is the same as the row length of the planting site, and when digging the trenches, the excavated soil is placed on both sides of the trenches to facilitate the subsequent backfilling of the trenches and covering the abandoned wolfberry branches or plant seedlings and corn stalks in the field.

[0046] In order to achieve laying of wolfberry discarded branches or weeds from plant seedlings in the field, and corn stalks inside the trench and determine the laying thickness, in the present embodiment, preferably, wolfberry discarded branches or weeds from plant seedlings in the field are laid at the bottom of the trench in S2, and then corn stalks are laid on the wolfberry discarded branches or weeds from plant seedlings in the field, and the wolfberry discarded branches or weeds from plant seedlings in the field, and corn stalks are laid in multiple layers, and the thickness of each layer is 5 cm, the thickness of the wolfberry discarded branches or weeds from plant seedlings in the field is 15-25 cm, and the thickness of the corn stalks is 10-15 cm, and after laying each layer of the wolfberry discarded branches or weeds from plant seedlings in the field, each layer is laid evenly and compacted.

[0047] In order to realize the activation culture of the strains and the reproduction of the strains, in this embodiment, preferably, the strains cultured in S3 are activated by refrigerated strains, that is, the strains in the preserved state are placed in a suitable culture medium for culture, and the culture is expanded step by step to obtain a pure and strong culture, that is, a culture with vigorous activity and sufficient inoculation quantity is obtained;

[0048] First, remove the cryopreservation tube from the cryopreservation condition;

[0049] Then place the cryopreservation tube on a small test tube rack in a 37°C bath, i.e., a 37°C, 70% alcohol bath, in which a small test tube rack is placed in advance, and the liquid level should not reach the tube stopper;

[0050] Then, shake the cryopreservation tube gently continuously, and the liquid level should not reach the tube stopper until the ice is completely dissolved to activate the bacteria.

[0051] Finally, sterile culture is carried out and the culture is expanded step by step to obtain a pure and strong culture.

[0052] In order to cover the abandoned branches of wolfberry or the weeds and corn stalks of plant seedlings in the field and facilitate watering, in this embodiment, preferably, the thickness of the backfilling in S4 is 10 cm, which can not only achieve complete coverage of the abandoned branches of wolfberry or the weeds and corn stalks of plant seedlings in the field, but also facilitate uniform watering. In addition, when backfilling, the straw must be exposed by 5-8 cm at both ends of the groove to facilitate watering and ventilation.

[0053] In order to determine the amount of bacterial strain to be spread, so as to facilitate the decomposition of discarded wolfberry branches or weeds from plant seedlings in the field and corn stalks, in this embodiment, preferably, the amount of discarded wolfberry branches or weeds from plant seedlings in the field in S2 is 2000-3000 kg per mu, the amount of corn stalks in the same proportion is 1300-1900 kg, and the amount of bacterial strain per mu is 8-10 kg.

[0054] In order to backfill the trench after watering and settlement, keep the trench flush with the ground, and carry out film covering and planting seedlings, in this embodiment, preferably, the ridge leveling in S5 is to backfill the upper surface of the trench again after watering, and backfill the settlement depth so that the upper surface of the trench can remain flush, and then cover the upper part of the trench with film, and fix the edge of the film with the soil layer, and finally select neat, healthy, virus-free and well-growing seedlings for planting.

[0055] In order to realize drilling holes at the positions of the planted seedlings and facilitate the planting of the seedlings, in this embodiment, preferably, the drilling in S6 is performed according to the determined distance and row of the planted seedlings, and the drilling depth must reach the straw layer, and about 14 round steel bars are used to drill oblique holes from the outside to the inside in the planting row to realize the planting of the seedlings.

[0056] The working principle and use process of the present invention:

[0057] The first step is to dig grooves in the unused shed during the winter: dig grooves in the unused shed during the winter, and the width of the grooves is 60-80cm and the depth of the grooves is 25-50cm;

[0058] The second step is to fill the trench with straw and waste: fill the bottom of the trench with a layer of discarded wolfberry branches or weeds from plant seedlings in the field, and then lay a layer of corn straw on the upper part of the discarded wolfberry branches or weeds from plant seedlings in the field;

[0059] Step 3: Evenly spread the fungus: evenly spread the straw and wolfberry waste, then take out the cultured fungus or directly purchase the ready-made fungus and evenly spread it on the discarded wolfberry branches or the plant seedlings in the field, and the upper part of the corn stalks;

[0060] Step 4: Backfill the soil: backfill the soil taken out from the trench and evenly cover the corn stalks;

[0061] Step 5: Watering and planting seedlings: After backfilling, water the soil and both ends of the corn stalks. Evenly water the discarded wolfberry branches or the fallen plant seedlings in the field and the corn stalks. After a few days, level the ridges, cover with film and plant seedlings.

[0062] Step 6. Drill holes in time after planting: Drill holes obliquely from the outside to the inside in the row of planted seedlings, and the depth of the holes should be enough to reach the corn stalk layer.

[0063] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An in-situ straw pellet bioreactor containing wolfberry waste, It is characterized in that The method includes making trenches in idle sheds during the winter, and stacking discarded wolfberry branches or weeds from plant seedlings in the field inside the trenches, and then placing corn straw on top of the discarded wolfberry branches or weeds from plant seedlings in the field, and sprinkling urea and bacteria. Finally, backfilling the soil, watering, covering the film and planting the seedlings, that is, increasing the concentration of carbon dioxide through wolfberry waste and bacteria, and under the same light, the photosynthetic efficiency is improved, and the soil temperature can be increased, and the shed temperature can also be increased. When the straw and waste are decomposed by the bacteria, a large number of disease-resistant organisms and their offspring can be bred, which effectively inhibits the growth of pathogens, and the decomposed straw and waste can provide organic matter.

2. An in-situ straw pellet bioreactor containing wolfberry waste according to claim 1, Features: The groove is used to collect data through a collection sensor, including temperature collection, humidity collection, carbon dioxide collection and light collection. The temperature collection includes soil layer temperature collection and ambient temperature collection. The collection sensor includes a filter. The filter adopts a second-order IIR low-pass filter in the IIR filter. The calculation formula of the second-order IIR low-pass filter is as follows: y(n) is the output data, x n is the input data, a and b are the coefficients of the filter, n is the number of filtering times, k=0 is the data information processed this time, k=1 is the data information processed next time, and nk is the position of the data information.

3. A method for an in-situ straw pellet bioreactor containing wolfberry waste, It is characterized in that The following steps are included: S1. Digging grooves in the unused sheds during the winter: digging grooves in the unused sheds during the winter, and the width of the grooves is 60-80cm and the depth of the grooves is 25-50cm; S2, filling the trench with straw and waste: filling the bottom of the trench with a layer of discarded wolfberry branches or weeds from plant seedlings in the field, and then laying a layer of corn straw on the upper part of the discarded wolfberry branches or weeds from plant seedlings in the field; S3, then evenly spread the fungus: spread the straw and wolfberry waste evenly and compactly, then take out the cultured fungus or directly purchase the ready-made fungus and evenly spread it on the discarded wolfberry branches or the plant seedlings in the field, and the upper part of the corn stalks; S4, backfilling: backfilling the soil taken out from the trench and evenly covering the corn stalks; S5, watering and planting seedlings: after soil backfilling, watering is performed, and both ends of the corn stalks are also watered, and the abandoned branches of wolfberry or the fallen seedlings of plants in the field and the corn stalks are evenly watered, and then the ridges are leveled, covered with film and the seedlings are planted after a few days; S6. Drill holes in time after transplanting: Drill holes obliquely from the outside to the inside in the row of transplanted seedlings, and the hole depth should reach the corn stalk layer.

4. The method of an in-situ straw pellet bioreactor containing wolfberry waste according to claim 3, Features: The trenches in S1 are dug along the planting rows, and the length of the trenches is the same as the row length of the planting field. When digging the trenches, the soil is placed on both sides of the trenches to facilitate subsequent backfilling of the trenches and covering the discarded wolfberry branches or fallen plant seedlings and corn stalks in the field.

5. The method of an in-situ straw pellet bioreactor containing wolfberry waste according to claim 3, Features: The bottom of the groove dug in S2 is laid with abandoned wolfberry branches or weeds from plant seedlings in the field, and then corn stalks are laid on the abandoned wolfberry branches or weeds from plant seedlings in the field, and the abandoned wolfberry branches or weeds from plant seedlings in the field and corn stalks are laid in multiple layers, and the thickness of each layer is 5 cm, the thickness of the abandoned wolfberry branches or weeds from plant seedlings in the field is 15-25 cm, and the thickness of the corn stalks is 10-15 cm, and after laying each layer of abandoned wolfberry branches or weeds from plant seedlings in the field and corn stalks in multiple layers, each layer is laid evenly and compacted.

6. The method of an in-situ straw pellet bioreactor containing wolfberry waste according to claim 3, Features: The cultured bacteria in S3 are activated by refrigerated bacteria, that is, the stored bacteria are placed in a suitable culture medium for culture, and the culture is expanded step by step to obtain a pure and strong culture, that is, a culture with vigorous activity and sufficient inoculation quantity; First, remove the cryopreservation tube from the cryopreservation condition; Then place the cryopreservation tube on a small test tube rack in a 37°C bath, i.e., a 37°C and 70% alcohol bath, in which a small test tube rack is placed in advance, and the liquid level should not reach the tube stopper; Then, shake the cryopreservation tube gently continuously, and the liquid level should not reach the tube stopper until the ice is completely dissolved to activate the bacteria. Finally, sterile culture is carried out and the culture is expanded step by step to obtain a pure and strong culture.

7. The method of an in-situ straw pellet bioreactor containing wolfberry waste according to claim 3, Features: The backfill thickness in S4 is 10 cm, which can not only completely cover the abandoned branches of wolfberry or the weeds and corn stalks in the field, but also facilitate uniform watering. When backfilling, the straw must be exposed 5-8 cm at both ends of the trench to facilitate watering and ventilation.

8. The method of an in-situ straw pellet bioreactor containing wolfberry waste according to claim 3, Features: The amount of discarded wolfberry branches or weeds from plant seedlings in the field in S2 is 2000-3000 kg per mu, the amount of corn stalks in the same proportion is 1300-1900 kg, and the amount of fungus per mu is 8-10 kg.

9. The method of an in-situ straw pellet bioreactor containing wolfberry waste according to claim 3, Features: The ridge leveling in S5 is to fill the upper surface of the groove with soil again after watering, and fill the depth of the settlement so that the upper surface of the groove can remain flush, and then cover the upper part of the groove with film, and press and fix the edge of the film with the soil layer, and finally select the seedlings that are neat, healthy, virus-free and well-growing for planting.

10. The method of an in-situ straw pellet bioreactor containing wolfberry waste according to claim 3, Features: The punching in S6 is performed according to the determined distance and row of the planted seedlings, and the punching depth should reach the straw layer, and about 14 round steel bars are used to punch holes obliquely from the outside to the inside in the planted row to achieve planting of the seedlings.

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