Tick-repelling composition and preparation method thereof

Abstract

The invention discloses a tick-repelling composition and a preparation method thereof, and the composition is prepared from the following components in parts by weight: 15 to 20 parts of N, N-diethyl-2-hydroxy-2-phenylacetamide, 12 to 16 parts of small molecule peptide composition and 1 to 3 parts of nanometer negative ion powder. The tick-repelling composition disclosed by the invention is remarkable in tick-repelling effect, free from toxic and side effects and good in skin care effect; in addition, in the preparation process, a low-temperature drying and air pressure superfine method is adopted, so that the medicine property is reserved to the maximum extent, the preparation process is easy to control, detonation is not prone to being generated, no residues, waste water and the like are discharged, and the preparation method is simple and environmentally friendly.

Description

Technical Field

[0001] The invention relates to the field of biotechnology, and in particular to a tick repellent composition and a preparation method thereof. Background Art

[0002] With global warming and the increasing complexity of the ecological environment, ticks, as carriers of many pathogens, have significantly increased their distribution range and activity. The infectious diseases caused by ticks, such as new viral infections, have become a major challenge in the field of public health.

[0003] As an important group of parasites in nature, ticks have biological characteristics that make them natural vectors of a variety of pathogens, posing a threat to human health, livestock, the ecological environment, and social economy that cannot be ignored. In terms of disease transmission, ticks can carry and spread a variety of pathogens including Lyme disease, forest encephalitis, and new viruses. These diseases not only have complex and diverse symptoms and are difficult to treat, but some diseases can rapidly deteriorate, seriously threatening the patient's life safety. In particular, new virus infections are hidden and highly pathogenic through tick bites, and the wounds are difficult to repair, making prevention and control work particularly difficult.

[0004] In terms of ecological impact, the massive reproduction of ticks not only directly harms host animals, such as high infection rates in wild animal populations, which may lead to a decline in their health and impaired reproductive capacity, thus affecting the stability and biodiversity of the entire ecosystem. The complex relationship between ticks and host animals may also spread through food chains and ecosystem networks, causing chain reactions to a wider range of biological communities.

[0005] From an economic perspective, the economic losses caused by tick-borne diseases should not be underestimated. The high cost of treatment for patients, especially the long-term medical expenses and rehabilitation costs for critically ill patients, imposes a heavy burden on individuals and families. Disease outbreaks may also affect the stability of the labor market, leading to a decline in productivity and hindered economic growth. At the same time, for industries such as tourism and animal husbandry that rely on natural landscapes and wildlife resources, the outbreak of tick epidemics may cause panic among tourists, reduce tourism activities, and further aggravate economic losses.

[0006] In terms of social impact, the frequent occurrence of tick-borne diseases can easily cause public panic and anxiety, and reduce the overall psychological security of society. The asymmetry of epidemic information and the spread of rumors may also aggravate social tensions and affect social stability and harmony. Therefore, strengthening the popularization of tick-borne diseases, raising public awareness of protection, and improving the epidemic monitoring and prevention and control system are of great significance for maintaining social stability and promoting the healthy development of society. Traditional chemical control relies on insecticides to act directly on ticks, killing them through neurotoxicity or physiological interference, which has the characteristics of fast effect, but long-term use is prone to environmental pollution and drug resistance. The development of tick repellent technology needs to take into account efficiency and sustainability. In the future, we should focus on the research and development of green, environmentally friendly, efficient and low-toxic chemical preparations with broad market value and application, which is of great significance. Summary of the invention

[0007] The main purpose of the present invention is to provide a tick repellent composition with significant tick repellent effect, no toxic side effects, and good skin care and repairing effect, and an environmentally friendly and simple preparation method thereof.

[0008] To achieve the above purpose, the technical solution adopted by the present invention provides a tick repellent composition, which includes the following components in parts by weight: 15-20 parts of N,N-diethyl-2-hydroxy-2-phenylacetamide, 12-16 parts of a small molecule peptide composition, and 1-3 parts of a nano negative ion powder; the small molecule peptide composition is composed of the following sequence: SEQ ID NO.1: LPKSALASILPEPSSPSK; SEQ ID NO.2: SIPKLKAEEPGSKLLLASPSG.

[0009] Furthermore, the preparation components of the tick repellent composition also include: the following components in parts by weight: 17 parts of N,N-diethyl-2-hydroxy-2-phenylacetamide, 14 parts of small molecule peptide composition, and 2 parts of nano negative ion powder.

[0010] Furthermore, the average particle size of the nano negative ion powder is 10-50nm.

[0011] Furthermore, the small molecule peptide composition is prepared by the following method: (1) Fasting, cleaning and dehydrating edible insects, and pre-treatment of heating the cleaned and dehydrated insect food with microwaves; (2) During the preheating and microwave treatment of the washed edible insects, the edible insects are subjected to hot air drying; (3) extracting fat from edible insects by suction, and then drying to obtain defatted powder; (4) Mix the defatted powder, pure water and insect enzymes and hydrolyze them under ultra-high pressure and low temperature; (5) The treated sample is centrifuged, and the obtained precipitate is freeze-dried to obtain the enzyme-treated material; (6) Dissolve the lyophilized crude product in water, separate and purify it, concentrate it, and freeze-dry it to obtain small molecule active peptides. Use high performance liquid chromatography-mass spectrometry to determine and select the small molecule sequence.

[0012] Furthermore, the method comprises the following steps: adding other components to N,N-diethyl-2-hydroxy-2-phenylacetamide and mixing them uniformly to obtain a mixture; uniformly mixing the mixture with water at a volume ratio of 1:50-100 and grinding the mixture into a slurry; and then low-temperature drying the mixture to a water content of 5-10wt% and crushing the mixture to 800-1000 meshes with ultra-micro wind pressure to obtain a tick repellent composition.

[0013] Furthermore, the composition is used in ointments, creams, sprays, essential oil dosage forms; and in animal husbandry and pet breeding.

[0014] Due to the application of the above technical solution, the present invention has the following advantages compared with the prior art: (1) The preparation method of the tick repellent composition provided by the present invention has a simple process, is easy to operate, and all the formula components are non-toxic and harmless to the human body; in addition, the preparation process is simple and has no waste discharge, has low dependence on production line requirements, has a short preparation cycle, is suitable for industrial production, and has a high value for promotion and application.

[0015] (2) The tick repellent composition provided by the present invention has a significant effect and no toxic side effects due to the interaction between the various components. The ultrafine powder technology is quickly soluble in water, and the small molecule peptide has strong skin care and repair ability. The nano-ion carrier makes the residual effect longer and better.

[0016] (3) The tick repellent composition provided by the present invention, the small molecule peptide composition, makes its function significant, has small toxic and side effects, interacts with each other, can better play a synergistic effect, and has strong skin care, repair and anti-inflammatory capabilities.

[0017] (4) The tick repellent composition provided by the present invention overcomes the defect that the previous dosage form of N,N-diethyl-2-hydroxy-2-phenylacetamide relies on high-concentration organic solvents. The new compound components have no toxic side effects and are safer and more environmentally friendly to use. DETAILED DESCRIPTION

[0018] The following description is used to disclose the present invention so that those skilled in the art can implement the present invention. The preferred embodiments described below are only examples, and those skilled in the art may think of other obvious variations. Example 1

[0019] A tick repellent composition comprises the following components by weight: 15 parts of N,N-diethyl-2-hydroxy-2-phenylacetamide, 12 parts of a small molecule peptide composition, and 1 part of a nano negative ion powder; the small molecule peptide composition is composed of the following sequence: SEQ ID NO.1: LPKSALASILPEPSSPSK. The average particle size of the nano negative ion powder is 50 nm. The other components are added to N,N-diethyl-2-hydroxy-2-phenylacetamide and mixed evenly to obtain a mixture, the mixture is mixed evenly with water at a volume ratio of 1:50, and then ground into a slurry, and then low-temperature dried to a water content of 5wt% and then crushed to 800 meshes by ultra-micro wind pressure to obtain a tick repellent composition. Example 2

[0020] A tick repellent composition comprises the following components by weight: 20 parts of N,N-diethyl-2-hydroxy-2-phenylacetamide, 16 parts of a small molecule peptide composition, and 3 parts of a nano anion powder; the small molecule peptide composition is composed of the following sequence: SEQ ID NO.2: SIPKLKAEEPGSKLLLASPSG. The average particle size of the nano anion powder is 50 nm.

[0021] Add other components to N,N-diethyl-2-hydroxy-2-phenylacetamide and mix them evenly to obtain a mixture. Mix the mixture evenly with water in a volume ratio of 1:100 and grind it into a slurry. Then, dry it at a low temperature to a water content of 10wt% and crush it to 1000 mesh with ultra-micro wind pressure to obtain a tick repellent composition. Example 3

[0022] The preparation method of the small molecule peptide composition is as follows: the larvae of the double star cricket and the white flower beetle are purchased from a professional breeding farm, fasted for 3 days respectively, and completely discharged waste 3 times. Then, the drying step, the defatting step, the crushing step, the high temperature / high pressure step, the enzyme hydrolysis step and the purification separation identification are completed to complete the manufacture of the ultra-high pressure enzyme treatment material.

[0023] (1) Drying stage: Drying in an industrial microwave dryer at 105°C for 5 minutes, and then drying in a hot air dryer at 60°C for 12 hours to reduce the moisture content of the larvae to less than 5%.

[0024] (2) Degreasing stage: using an industrial milking machine (Mingjin Machinery) at 600kgf / cm 2 Milking was carried out at a pressure of 200 °C for 20 min to achieve a fat-free state. The matrix (sample volume) was 2 kg and the matrix (in the milking machine) temperature was 60 °C.

[0025] (3) Crushing stage: The brown mealworm larvae are crushed to a particle size of 50-60 mesh using an industrial grinder to obtain a powdery powder.

[0026] Then, 100 weight parts of larval defatted powder, 300 weight parts of distilled water, and 1.0 weight part (vol%) of Alcalase (subtilisin Carlsberg, Novozymes) were placed in a sealed container, and then the sealed container was placed in an ultra-high pressure container (055-60, Uhde, Germany), and then the lid was closed and sealed. After the inside of the pressure container was filled with water, the airtight container was pressurized from low temperature to ultra-high pressure using the pressure of water pressure as a medium in water under a vacuum state without contact with air. At this time, the pressure was maintained at 100 MPa, and the temperature inside the pressurized container was set at 50 ° C, and the hydrolysis process was carried out for 24 hours. After centrifuging the sample, the supernatant was freeze-dried to obtain the enzyme treatment.

[0027] Edible insect enzyme-treated products were prepared, but the temperature during ultrahigh pressure and low temperature treatment was set to 50°C.

[0028] Edible insect enzyme-treated products were prepared, but the pressure during ultra-high pressure and low temperature treatment was set to 100 MPa.

[0029] Edible insect enzyme-treated products were prepared, but the pressure during ultra-high pressure and low temperature treatment was set to 200 MPa.

[0030] The lyophilized crude product was dissolved in water, separated and purified, concentrated to 100 mL using a 1000 kD hollow fiber membrane column, purified by an anion exchange chromatography column, and sterilized by filtration through a 0.1 µm membrane to obtain a sterile small molecule active peptide.

[0031] After concentration, freeze-drying was performed to obtain small molecule active peptides, and the small molecule sequence was selected by high performance liquid chromatography-mass spectrometry. The small molecule peptides collected for purity and amino acid sequence determination were detected by liquid chromatography peak detection, and further analyzed by mass spectrometer, and analyzed and identified by mass spectrometry software. The mass spectrometry results were used to blast the substances, analyze and identify them, and identify a variety of peptide segments, from which short peptides with safety and easy absorption were selected as follows: The polypeptide is preferably selected from the following amino acid sequences: SEQ ID NO. 1: LPKSALASILPEPSSPSK, SEQ ID NO. 2: SIPKLKAEEPGSKLLLASPSG. Example 4

[0032] N,N-diethyl-2-hydroxy-2-phenylacetamide is added with water in a volume ratio of 1:100, mixed evenly, ground into slurry, and then low-temperature dried to a water content of 10wt% and crushed to 1000 mesh by ultra-micro wind pressure to obtain a tick repellent composition.

[0033] Comparative Example 1 This example provides a commercially available tick repellent composition, which is basically the same as Example 1, except that N,N-diethyl-2-hydroxy-2-phenylacetamide and small molecule peptide are not added.

[0034] Comparative Example 2 This example provides a tick repellent composition, which is basically the same as Example 1, except that no small molecule peptide composition is added.

[0035] Comparative Example 3 This example provides a tick repellent composition, which is basically the same as Example 1, except that no nano negative ion powder is added. The above Examples 1-4 and Comparative Examples 1-3 were tested for relevant properties, and the test results are shown in Table 1. The test method is: using QB / T2738-2012, etc.

[0036] It can be seen from Table 1 that the tick repellent compositions in Examples 1 and 2 of the present application have better tick repellent effects, have the effect of repairing skin wounds and have no residual toxicity that causes death. This is the result of the synergistic effect of the components. The addition of N,N-diethyl-2-hydroxy-2-phenylacetamide and the small molecule peptide composition is beneficial to improving the above-mentioned properties.

[0037] Table 1 project Skin repair rate Tick ​​repellency rate Delay rate Residual toxicity fatality rate unit % % sky % Example 1 91 98.00 7 0 Example 2 92 98.00 7 0 Example 4 10 90.00 2 0 Comparative Example 1 16 61.00 2 30 Comparative Example 2 14 96.00 7 0 Comparative Example 3 75 80.00 2 0 The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The above embodiments and descriptions only describe the principles of the present invention. The present invention may be subject to various changes and improvements without departing from the spirit and scope of the present invention. These changes and improvements fall within the scope of the present invention. The scope of protection claimed by the present invention is defined by the attached claims and their equivalents.

Claims

1. A tick repellent composition, characterized in that: The invention comprises the following components: N,N-diethyl-2-hydroxy-2-phenylacetamide.

2. A tick repellent composition, characterized in that: The invention comprises the following components: N, N-diethyl-2-hydroxy-2-phenylacetamide, a small molecule peptide composition and nanometer negative ion powder.

3. A tick repellent composition, characterized in that: The invention comprises the following components in parts by weight: 15-20 parts of N,N-diethyl-2-hydroxy-2-phenylacetamide, 12-16 parts of small molecule peptide composition and 1-3 parts of nanometer negative ion powder.

4. The tick repellent composition according to claim 3, characterized in that: The small molecule peptide composition is the following sequence Composition: SEQ ID NO.1: LPKSALASILPEPSSPSK, SEQ ID NO.2: SIPKLKAEEPGSKLLLASPSG.

5. The tick repellent composition according to claim 3, characterized in that: The preparation components of the tick repellent composition are: 17 parts of N,N-diethyl-2-hydroxy-2-phenylacetamide, 14 parts of a small molecule peptide composition, and 2 parts of nano negative ion powder.

6. The tick repellent composition according to claim 3, characterized in that: The average particle size of the nano negative ion powder is 10-50nm.

7. The tick repellent composition according to claim 3, characterized in that: The small molecule peptide composition is prepared by the following method: (1) fasting, cleaning and dehydrating edible insects, and pre-treating the cleaned and dehydrated insect food by heating with microwaves; (2) During the preheating and microwave treatment of the washed edible insects, the edible insects are subjected to hot air drying; (3) extracting fat from edible insects by suction, and then drying to obtain defatted powder; (4) Mix the defatted powder, pure water and insect enzymes and hydrolyze them under ultra-high pressure and low temperature; (5) The treated sample is centrifuged, and the obtained precipitate is freeze-dried to obtain the enzyme-treated material; (6) Dissolve the lyophilized crude product in water, separate and purify it, concentrate it, and freeze-dry it to obtain small molecule active peptides. Use high performance liquid chromatography-mass spectrometry to determine and select the small molecule sequence.

8. The method for preparing the tick repellent composition according to claim 3, characterized in that: The method comprises the following steps: adding a small molecule peptide composition and nanometer negative ion powder to N,N-diethyl-2-hydroxy-2-phenylacetamide and mixing evenly to obtain a mixture; mixing the mixture evenly with water at a volume ratio of 1:50-100, grinding the mixture into a slurry, and then low-temperature drying the mixture to a water content of 5-10wt% and then crushing the mixture to 800-1000 meshes with ultra-micro wind pressure to obtain a tick repellent composition.

9. The use of the composition according to claims 1-7, characterized in that: The composition is used in ointments, creams, sprays and essential oil dosage forms.

10. The use of the composition according to claims 1-7, characterized in that: The composition is used in animal husbandry and pet breeding.