Sample extraction device for stem cell preparation

Abstract

The invention relates to the technical field of stem cell extraction devices, in particular to a sample extraction device for stem cell preparation, which comprises a sampling bottle, a sampling tube connected to the inner wall of the center of the bottom of the sampling bottle, a mounting ring connected to the bottom end of the outer wall of the sampling bottle, a bottom cover connected to the bottom of the mounting ring, and an alcohol cotton sleeve connected to the inner wall of the center of the top of the bottom cover. The bottom of the alcohol cotton sleeve is connected with a bottom block, and the inner wall of the center of the top of the bottom block is connected with a rubber plug. Through a simple connection mode between the mounting ring and the sampling bottle, the dismounting step of the bottom cover is more convenient and quicker, and meanwhile, the extraction device is also convenient to use; the sampling tube has the advantages that the sampling tube is provided with the dustproof function and the sterilization and disinfection effect, bacteria are prevented from being attached to the sampling tube, the bottom end of the sampling tube can be blocked by the rubber plug, and stem cells are prevented from being squeezed out.

Description

Technical Field

[0001] The present invention relates to the technical field of stem cell extraction devices, and in particular to a sample extraction device for stem cell preparation. Background Art

[0002] Stem cells are a type of multipotent cells with the ability to self-renew and self-replicate. Stem cells can be divided into embryonic stem cells and adult stem cells. According to different differentiation potentials, they can be divided into totipotent stem cells, pluripotent stem cells, and unipotent stem cells. They have the characteristics of multidirectional differentiation potential, unlimited division and proliferation, etc.

[0003] The following problems exist: a sample extraction device is usually used to extract stem cells from a culture dish. Before the sample extraction device is used, the sampling tube portion of the extraction device is exposed to the air for a long time, so bacteria in the air will adhere to the sampling tube portion. When in use, the bacteria will enter the sample extraction device and contaminate the stem cells. At the same time, after the sample extraction device is used, if the operator presses the pressure plate by mistake, the stem cells in the sample extraction device will be squeezed out.

[0004] In view of this, research and improvement are conducted on the existing problems, and a sample extraction device for stem cell preparation is provided. By setting a simple connection method between the mounting ring and the sampling bottle, the disassembly and assembly steps of the bottom cover are more convenient and quick, and the extraction device is also convenient to use. By setting a connection structure between the alcohol cotton sleeve and the sampling tube, it not only plays a dust-proof role, but also has a sterilization effect, thereby preventing bacteria from adhering to the sampling tube. By setting a rubber stopper, the bottom end of the sampling tube can be sealed, thereby avoiding the situation where stem cells are squeezed out. The purpose is to achieve the purpose of solving problems and improving practical value through this technology. Summary of the invention

[0005] The purpose of the present invention is to solve the shortcomings of the prior art and to propose a sample extraction device for stem cell preparation.

[0006] In order to achieve the above-mentioned purpose, the present invention adopts the following technical scheme: a sample extraction device for preparing stem cells, comprising a sampling bottle, a sampling tube is connected to the inner wall at the center of the bottom of the sampling bottle, a mounting ring is connected to the bottom end of the outer wall of the sampling bottle, a bottom cover is connected to the bottom of the mounting ring, an alcohol cotton sleeve is connected to the inner wall at the center of the top of the bottom cover, a bottom block is connected to the bottom of the alcohol cotton sleeve, a rubber plug is connected to the inner wall at the center of the top of the bottom block, a top cover is connected to the top of the inner wall of the sampling bottle, a push-pull rod is connected to the inner wall of the top cover, a rubber piston is connected to the bottom of the push-pull rod, a pressure plate is connected to the top of the push-pull rod, a protective sleeve is connected to the outer wall of the sampling bottle, a support ring is connected to the top of the outer wall of the sampling bottle, a threaded shaft is connected to one end of the inner wall of the support ring, a graduated tube is connected to the top of the threaded shaft, a threaded rod is connected to the other end of the inner wall of the support ring, a first nut is connected to the top of the outer wall of the threaded rod, and a second nut is connected to the bottom end of the outer wall of the threaded rod.

[0007] As a further description of the above technical solution:

[0008] The inner wall at the bottom center of the sampling bottle is fixedly connected to the top of the outer wall of the sampling tube, and the bottom end of the outer wall of the sampling bottle is threadedly connected to the inner wall of the mounting ring; by setting a simple connection method between the mounting ring and the sampling bottle, the disassembly and assembly steps of the bottom cover are more convenient and quick, and it is also convenient to use the extraction device.

[0009] As a further description of the above technical solution:

[0010] The bottom of the mounting ring is fixedly connected to the top of the bottom cover, the inner wall at the center of the top of the bottom cover is fixedly connected to the outer wall of the alcohol cotton sleeve, and the inner wall of the alcohol cotton sleeve is sleeved with the bottom end of the outer wall of the sampling tube; by setting the connection structure between the alcohol cotton sleeve and the sampling tube, it not only plays a dust-proof role, but also has the effect of sterilization and disinfection, thereby preventing bacteria from adhering to the sampling tube.

[0011] As a further description of the above technical solution:

[0012] The bottom of the alcohol cotton sleeve is fixedly connected to the top of the bottom block, the outer wall of the bottom block is fixedly connected to the inner wall at the center of the bottom of the bottom cover, and the inner wall at the center of the top of the bottom block is bonded to the outer wall of the rubber plug; by setting the rubber plug, the bottom end of the sampling tube can be sealed to avoid the situation where stem cells are squeezed out.

[0013] As a further description of the above technical solution:

[0014] The top of the inner wall of the sampling bottle is threadedly connected to the bottom end of the outer wall of the top cover, the inner wall of the top cover is sleeved with the outer wall of the push-pull rod, the bottom of the push-pull rod is fixedly connected to the center of the top of the rubber piston, and the outer wall of the rubber piston is sleeved with the bottom end of the inner wall of the sampling bottle; by setting a simple connection method between the top cover and the sampling bottle, the disassembly and assembly steps of the top cover are more convenient and quick, and it is also convenient to clean the inside of the sampling bottle.

[0015] As a further description of the above technical solution:

[0016] The top of the push-pull rod is bonded to the center of the bottom of the pressure plate, and the outer wall of the sampling bottle is sleeved with the inner wall of the protective sleeve; by providing the protective sleeve, the surface of the sampling bottle can be protected to prevent damage to the sampling bottle, and by providing a simple connection method between the protective sleeve and the sampling bottle, the steps of replacing, cleaning and sterilizing the protective sleeve are more convenient and quick.

[0017] As a further description of the above technical solution:

[0018] The top of the outer wall of the sampling bottle is fixedly connected to the inner wall of the support ring, one end of the inner wall of the support ring is threadedly connected to the outer wall of the threaded shaft, and the top of the threaded shaft is bonded to the center of the bottom of the calibrated tube; by setting the calibrated tube, the position of the pressure plate can be measured.

[0019] As a further description of the above technical solution:

[0020] The outer wall of the calibrated tube is sleeved with one end of the inner wall of the pressure plate, one end of the outer wall of the threaded rod is threadedly connected with the other end of the inner wall of the support ring, and the other end of the outer wall of the threaded rod is sleeved with the other end of the inner wall of the pressure plate; by setting the connecting structure between the pressure plate and the calibrated tube, and the pressure plate and the threaded rod, the pressure plate can move up and down on the outer walls of the calibrated tube and the threaded rod.

[0021] As a further description of the above technical solution:

[0022] The top end of the outer wall of the threaded rod is threadedly connected to the inner wall of the first nut, and the bottom end of the outer wall of the threaded rod is threadedly connected to the inner wall of the second nut; by setting the first nut and the second nut structure, the threaded rod can be fixed on the support ring, and the length above the threaded rod can be adjusted and fixed, so that the up and down movement distance of the pressure plate can be limited. At this time, the extraction amount of stem cells can be controlled, thereby avoiding waste of stem cells.

[0023] The present invention has the following beneficial effects:

[0024] 1. By setting a simple connection method between the mounting ring and the sampling bottle, the disassembly and assembly steps of the bottom cover are more convenient and quick, and it is also convenient to use the extraction device. By setting a connection structure between the alcohol cotton sleeve and the sampling tube, it not only plays a dust-proof role, but also has a sterilization effect, thereby preventing bacteria from adhering to the sampling tube. By setting a rubber stopper, the bottom end of the sampling tube can be blocked, thereby preventing the stem cells from being squeezed out.

[0025] 2. By setting a simple connection method between the top cover and the sampling bottle, the disassembly and assembly steps of the top cover are more convenient and quick, and it is also convenient to clean the inside of the sampling bottle. By setting a protective cover, the surface of the sampling bottle can be protected to prevent damage to the sampling bottle. By setting a simple connection method between the protective cover and the sampling bottle, the replacement, cleaning and sterilization steps of the protective cover are more convenient and quick.

[0026] 3. By setting a graduated tube, the position of the pressure plate can be measured. By setting a connecting structure between the pressure plate and the graduated tube, and between the pressure plate and the threaded rod, the pressure plate can move up and down on the outer walls of the graduated tube and the threaded rod. By setting a first nut and a second nut structure, the threaded rod can be fixed on the support ring. By adjusting and fixing the length above the threaded rod, the up and down moving distance of the pressure plate can be limited. At this time, the extraction amount of stem cells can be controlled, thereby avoiding waste of stem cells. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 A three-dimensional diagram of a sample extraction device for stem cell preparation proposed by the present invention;

[0028] Figure 2 This is a front view of a sample extraction device for stem cell preparation proposed by the present invention;

[0029] Figure 3 This is a front cross-sectional view of a sample extraction device for stem cell preparation proposed by the present invention;

[0030] Figure 4 A sample extraction device for stem cell preparation proposed by the present invention Figure 3 A partial enlarged view of the middle A area;

[0031] Figure 5 A sample extraction device for stem cell preparation proposed by the present invention Figure 3 A partial enlarged view of the middle B area;

[0032] Figure 6 A sample extraction device for stem cell preparation proposed by the present invention Figure 3 A partial enlarged view of area C in the middle.

[0033] Legend:

[0034] 1. Sampling bottle; 2. Sampling tube; 3. Mounting ring; 4. Bottom cover; 5. Alcohol cotton sleeve; 6. Bottom block; 7. Rubber plug; 8. Top cover; 9. Push-pull rod; 10. Rubber piston; 11. Pressure plate; 12. Protective cover; 13. Support ring; 14. Threaded shaft; 15. Scale tube; 16. Threaded rod; 17. First nut; 18. Second nut. DETAILED DESCRIPTION

[0035] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0036] In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inside", "outside" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific position, be constructed and operated in a specific position, and therefore cannot be understood as limiting the present invention; the terms "first", "second", and "third" are only used for descriptive purposes, and cannot be understood as indicating or implying relative importance. In addition, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be an indirect connection through an intermediate medium, or it can be a connection between the two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0037] Reference Figure 1-6The present invention provides an embodiment: a sample extraction device for preparing stem cells, comprising a sampling bottle 1, a sampling tube 2 is connected to the inner wall at the bottom center of the sampling bottle 1, a mounting ring 3 is connected to the bottom end of the outer wall of the sampling bottle 1, a bottom cover 4 is connected to the bottom of the mounting ring 3, an alcohol cotton sleeve 5 is connected to the inner wall at the top center of the bottom cover 4, a bottom block 6 is connected to the bottom of the alcohol cotton sleeve 5, a rubber stopper 7 is connected to the inner wall at the top center of the bottom block 6, a top cover 8 is connected to the top of the inner wall of the sampling bottle 1, and the inner wall of the top cover 8 is connected to A push-pull rod 9, a rubber piston 10 is connected to the bottom of the push-pull rod 9, a pressure plate 11 is connected to the top of the push-pull rod 9, a protective sleeve 12 is connected to the outer wall of the sampling bottle 1, a support ring 13 is connected to the top of the outer wall of the sampling bottle 1, a threaded shaft 14 is connected to one end of the inner wall of the support ring 13, a graduated tube 15 is connected to the top of the threaded shaft 14, a threaded rod 16 is connected to the other end of the inner wall of the support ring 13, a first nut 17 is connected to the top of the outer wall of the threaded rod 16, and a second nut 18 is connected to the bottom end of the outer wall of the threaded rod 16.

[0038] The inner wall at the bottom center of the sampling bottle 1 is fixedly connected to the top of the outer wall of the sampling tube 2, the bottom end of the outer wall of the sampling bottle 1 is threadedly connected to the inner wall of the mounting ring 3, the bottom of the mounting ring 3 is fixedly connected to the top of the bottom cover 4, the inner wall at the top center of the bottom cover 4 is fixedly connected to the outer wall of the alcohol cotton sleeve 5, the inner wall of the alcohol cotton sleeve 5 is sleeved with the bottom end of the outer wall of the sampling tube 2, the bottom of the alcohol cotton sleeve 5 is fixedly connected to the top of the bottom block 6, the outer wall of the bottom block 6 is fixedly connected to the inner wall at the bottom center of the bottom of the bottom cover 4, the inner wall at the top center of the bottom block 6 is bonded to the outer wall of the rubber plug 7, the top end of the inner wall of the sampling bottle 1 is threadedly connected to the bottom end of the outer wall of the top cover 8, the inner wall of the top cover 8 is sleeved with the outer wall of the push-pull rod 9, the bottom of the push-pull rod 9 is fixedly connected to the top center of the rubber piston 10, the rubber piston 1 0 outer wall is sleeved with the bottom end of the inner wall of the sampling bottle 1, the top of the push-pull rod 9 is bonded to the bottom center of the pressure plate 11, the outer wall of the sampling bottle 1 is sleeved with the inner wall of the protective sleeve 12, the top of the outer wall of the sampling bottle 1 is fixedly connected with the inner wall of the support ring 13, one end of the inner wall of the support ring 13 is threadedly connected with the outer wall of the threaded shaft 14, the top of the threaded shaft 14 is bonded to the bottom center of the graduated tube 15, the outer wall of the graduated tube 15 is sleeved with one end of the inner wall of the pressure plate 11, one end of the outer wall of the threaded rod 16 is threadedly connected with the other end of the inner wall of the support ring 13, the other end of the outer wall of the threaded rod 16 is sleeved with the other end of the inner wall of the pressure plate 11, the top of the outer wall of the threaded rod 16 is threadedly connected with the inner wall of the first nut 17, and the bottom end of the outer wall of the threaded rod 16 is threadedly connected with the inner wall of the second nut 18.

[0039] Working principle and process: first remove the second nut 18, then slowly turn the first nut 17 upwards, and at the same time turn the threaded rod 16, so that the rotating threaded rod 16 slowly moves up and down at the other end of the inner wall of the support ring 13. When the threaded rod 16 moves upward, the range of movement of the pressure plate 11 will become larger, and the amount of stem cell extraction will increase. When the threaded rod 16 moves downward, the range of movement of the pressure plate 11 will become smaller, and the amount of stem cell extraction will decrease. By relying on the graduated tube 15 at the top of the threaded shaft 14, the specific range of movement of the pressure plate 11 can be known, and the extraction of stem cells can also be clearly known. The specific amount to be taken is determined by the following steps: when the threaded rod 16 does not need to be rotated, the first nut 17 can be rotated to the top of the support ring 13, and the second nut 18 can be installed at the bottom of the threaded rod 16. At the same time, the second nut 18 can be rotated to the bottom of the support ring 13. When the first nut 17 and the second nut 18 moving towards each other can no longer rotate, the entire threaded rod 16 is fixed. The alcohol cotton sleeve 5 is used to not only prevent dust, but also to sterilize and disinfect. The rubber plug 7 on the inner wall of the bottom block 6 can seal the bottom of the sampling tube 2 to prevent the stem cells from being squeezed out.

[0040] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned embodiments or to make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A sample extraction device for stem cell preparation, comprising a sampling bottle (1), characterized in that: The inner wall at the bottom center of the sampling bottle (1) is connected to a sampling tube (2); the bottom end of the outer wall of the sampling bottle (1) is connected to a mounting ring (3); the bottom of the mounting ring (3) is connected to a bottom cover (4); the inner wall at the top center of the bottom cover (4) is connected to an alcohol cotton sleeve (5); the bottom of the alcohol cotton sleeve (5) is connected to a bottom block (6); the inner wall at the top center of the bottom block (6) is connected to a rubber stopper (7); the top of the inner wall of the sampling bottle (1) is connected to a top cover (8); the inner wall of the top cover (8) is connected to a push-pull rod (9); the bottom of the push-pull rod (9) is connected to a rubber stopper (7); The push-pull rod (9) is provided with a pressure plate (11) at the top, the outer wall of the sampling bottle (1) is connected with a protective sleeve (12), the top of the outer wall of the sampling bottle (1) is connected with a support ring (13), one end of the inner wall of the support ring (13) is connected with a threaded shaft (14), the top of the threaded shaft (14) is connected with a graduated tube (15), the other end of the inner wall of the support ring (13) is connected with a threaded rod (16), the top of the outer wall of the threaded rod (16) is connected with a first nut (17), and the bottom of the outer wall of the threaded rod (16) is connected with a second nut (18).

2. A sample extraction device for stem cell preparation according to claim 1, characterized in that: The inner wall at the bottom center of the sampling bottle (1) is fixedly connected to the top of the outer wall of the sampling tube (2), and the bottom end of the outer wall of the sampling bottle (1) is threadedly connected to the inner wall of the mounting ring (3).

3. A sample extraction device for preparing stem cells according to claim 1, characterized in that: The bottom of the mounting ring (3) is fixedly connected to the top of the bottom cover (4), the inner wall at the center of the top of the bottom cover (4) is fixedly connected to the outer wall of the alcohol cotton sleeve (5), and the inner wall of the alcohol cotton sleeve (5) is sleeved with the bottom end of the outer wall of the sampling tube (2).

4. A sample extraction device for preparing stem cells according to claim 1, characterized in that: The bottom of the alcohol cotton sleeve (5) is fixedly connected to the top of the bottom block (6), the outer wall of the bottom block (6) is fixedly connected to the inner wall at the center of the bottom of the bottom cover (4), and the inner wall at the center of the top of the bottom block (6) is bonded to the outer wall of the rubber stopper (7).

5. The sample extraction device for preparing stem cells according to claim 1, characterized in that: The top end of the inner wall of the sampling bottle (1) is threadedly connected to the bottom end of the outer wall of the top cover (8), the inner wall of the top cover (8) is sleeved with the outer wall of the push-pull rod (9), the bottom of the push-pull rod (9) is fixedly connected to the center of the top of the rubber piston (10), and the outer wall of the rubber piston (10) is sleeved with the bottom end of the inner wall of the sampling bottle (1).

6. A sample extraction device for preparing stem cells according to claim 1, characterized in that: The top of the push-pull rod (9) is bonded to the center of the bottom of the pressure plate (11), and the outer wall of the sampling bottle (1) is sleeved with the inner wall of the protective sleeve (12).

7. The sample extraction device for preparing stem cells according to claim 1, characterized in that: The top of the outer wall of the sampling bottle (1) is fixedly connected to the inner wall of the support ring (13), one end of the inner wall of the support ring (13) is threadedly connected to the outer wall of the threaded shaft (14), and the top of the threaded shaft (14) is bonded to the center of the bottom of the graduated tube (15).

8. The sample extraction device for preparing stem cells according to claim 1, characterized in that: The outer wall of the graduated tube (15) is sleeved with one end of the inner wall of the pressure plate (11), one end of the outer wall of the threaded rod (16) is threadedly connected with the other end of the inner wall of the support ring (13), and the other end of the outer wall of the threaded rod (16) is sleeved with the other end of the inner wall of the pressure plate (11).

9. The sample extraction device for preparing stem cells according to claim 1, characterized in that: The top end of the outer wall of the threaded rod (16) is threadedly connected to the inner wall of the first nut (17), and the bottom end of the outer wall of the threaded rod (16) is threadedly connected to the inner wall of the second nut (18).

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