Transfer factor detection equipment and method

Abstract

The invention discloses a transfer factor detection device and method, and relates to the technical field of biological detection.The transfer factor detection device comprises a microplate reader, a detection chamber, a microwell plate and a detection component, sliding grooves are symmetrically formed in the inner wall of the detection chamber, trays are slidably connected to the inner walls of the sliding grooves in an attached mode, a pushing positioning mechanism is arranged at the bottom of each tray, and the pushing positioning mechanisms are arranged on the inner walls of the detection chambers; and during pushing detection, the detection position of the microwell plate is attached and fixed. According to the transfer factor detection equipment, in the pushing detection process, a tray can be pushed to drive a large gear to rotate, and four positioning blocks synchronously move inwards through cooperation of a guide groove and a shaft rod, so that four side walls of a microplate can be attached and positioned through the four positioning blocks when the microplate is pushed to a detection position; and the accuracy of the position of the microplate in the detection process is ensured, so that the accuracy of the subsequent detection result of the equipment is ensured.

Description

Technical Field

[0001] The present invention relates to the field of biological detection technology, in particular to a detection device and method for transfer factors. Background Art

[0002] With the development of modern breeding industry and the continuous improvement of scale and intensiveness, diseases have become a problem that plagues the breeding industry. The current common solution is immunity. However, due to various reasons, it is sometimes inevitable that immune failure will occur, and atypical infectious diseases will break out, which brings certain difficulties to diagnosis and treatment. Transfer factor is a factor released by immune-active T lymphocytes in the body that can transfer immune sensitization information. It can specifically transfer a certain cellular immune function of the donor to the recipient animal, non-specifically enhance the immune function of the recipient animal, and is a new type of immune stimulant. It is widely used to enhance the immune function of poultry and prevent and treat viral diseases.

[0003] After injection, since transfer factor is an important immune regulatory factor in poultry, it is necessary to detect the level of transfer factor, which can evaluate whether the immune function of poultry is sound, so as to timely discover potential immunity. Current detection methods include enzyme-linked immunosorbent assay, immunoblotting, mass spectrometry, etc. Among them, enzyme-linked immunosorbent assay is widely used due to its high sensitivity, high specificity and simple operation. Enzyme label analyzer, also known as enzyme-linked immunosorbent assay, is a special instrument for enzyme-linked immunosorbent assay, also known as microplate detector. It uses colorimetry for analysis and is used in combination with adapter reagents for qualitative and quantitative analysis of transfer factors in biological samples.

[0004] Currently, when using an ELISA reader, the operator will place the microplate containing the enzyme on the pulled-out tray, and then push the tray into the ELISA reader to detect the transfer factor in the sample. The operator will position it when placing it, but the microplate lacks positioning components during the pushing of the tray, so the position accuracy of the microplate in the ELISA reader cannot be guaranteed, which may cause the microplate to tilt or shift. At the same time, due to the instability of the microplate during movement, the solution loaded inside is easy to overflow and mix with the solutions of other microwells to cause contamination, thereby affecting the accuracy of subsequent ELISA test results. Summary of the invention

[0005] The object of the present invention is to provide a transfer factor detection device and method to solve the problems raised in the above background technology.

[0006] To achieve the above object, the present invention provides a transfer factor detection device as follows, comprising an ELISA instrument, a detection chamber, a microplate, and a detection component, wherein the inner wall of the detection chamber is symmetrically provided with a slide groove, and the inner wall of the slide groove is slidably connected with a tray;

[0007] A push positioning mechanism is provided at the bottom of the tray to fit and fix the detection position of the microplate during push detection;

[0008] A push protection mechanism is symmetrically arranged on the top of the tray to block the upper surface of the microplate during push detection;

[0009] The inner wall of the detection chamber is symmetrically provided with a push-pull cleaning mechanism to clean the blocking surface before and after blocking during push detection.

[0010] Preferably, the pushing and positioning mechanism includes a main rod, and the top of the main rod is rotatably connected to the bottom of the tray, the bottom of the main rod is fixedly connected to a large gear, and a small gear is meshed on one side of the large gear, the top of the small gear is fixedly connected to a secondary rod, and the secondary rod is rotatably connected to the bottom of the tray, and a rack is fixedly connected to the inner wall of one side of the detection chamber corresponding to the small gear, and one side of the small gear is meshed and matched with the tooth groove of the rack.

[0011] Preferably, guide grooves are equidistantly penetrated through the top of the large gear, and the inner wall of the guide groove is slidably connected to a shaft rod, a positioning block is fixedly connected to the top of the shaft rod, a limiting groove is penetrated through the corresponding positioning block on the top of the tray, and the outer walls on both sides of the positioning block slide in fit with the inner wall of the limiting groove, support grooves are symmetrically provided on the inner wall of the limiting groove, and support blocks are fixedly connected to the outer walls on both sides of the positioning block corresponding to the support grooves, and the outer walls of the support blocks slide in fit with the inner wall of the support groove.

[0012] Preferably, the pushing protection mechanism includes a first fixed block, and the bottom of the first fixed block is fixedly connected to the top of the tray, the inside of the first fixed block is slidably connected to a rotating rod, and the outer wall of the other end of the rotating rod is slidably connected to a second fixed block, the bottom of the second fixed block is fixedly connected to the top of the tray, the outer wall of the rotating rod is fixedly connected to a baffle, the tops of the two baffles are inclined, and the side of the two baffles close to each other is sprayed with a nano-coating, and the outer wall of the end of the rotating rod close to the second fixed block is fitted with a first spring.

[0013] Preferably, one end of the first spring is fixedly connected to a side wall of the second fixed block, and the other end of the first spring is fixedly connected to a supporting ring, a rotating groove is provided on an outer wall of one end of the rotating rod close to the second fixed block corresponding to the supporting ring, and the inner wall of the supporting ring rotates in fit with the inner wall of the rotating groove, a clamping strip is fixedly connected to the outer wall of one end of the rotating rod close to the first fixed block, and a clamping strip is provided on a side wall of the first fixed block away from the second fixed block corresponding to the clamping strip, and the outer wall of the clamping strip slides in fit with the inner wall of the clamping slot.

[0014] Preferably, torsion springs are arranged at equal distances on the outer wall of the rotating rod, and one end of the torsion spring is overlapped on the outer wall of the rotating rod, and the other ends of the two torsion springs are respectively overlapped on the side wall close to the first fixed block and the second fixed block, and a side wall of the first fixed block away from the second fixed block is fixedly connected with a card column corresponding to the card strip, and a side surface away from the two baffles is fixedly connected with a trigger top block corresponding to the push-pull cleaning mechanism.

[0015] Preferably, the push-pull cleaning mechanism includes a sliding rod, and the two ends of the sliding rod are fixedly connected to the inner wall of the detection chamber, the outer wall of the sliding rod is slidably connected to a slider, and a side wall of the slider is provided with a mounting groove, the inner wall of the mounting groove is slidably connected to a mounting block, and the bottom of the mounting block is fixedly connected to a cleaning block, a cleaning cavity is penetrated through one side wall of the cleaning block corresponding to the baffle, and the inner wall of the cleaning cavity slides in contact with the outer wall of the baffle, and the inner wall of the cleaning cavity is provided with an electrostatic adsorption film.

[0016] Preferably, a second spring is disposed on the outer wall of the slide rod, one end of the second spring is fixedly connected to the inner wall of the detection chamber, and the other end of the second spring is fixedly connected to a side wall of the slide block.

[0017] Preferably, a support plate is fixedly mounted on one side of the tray, and a handle is fixedly mounted on one side of the support plate.

[0018] The present invention provides a transfer factor detection method as follows, comprising the following steps:

[0019] Push positioning: first pull the tray out of the test chamber, then carefully place the microplate in the center of the tray, manually press the two baffles to fit the upper surface of the microplate, and then push the tray back into the test chamber. At this time, the four positioning blocks move inward synchronously during the pushing process, so that when it is pushed to the test position, the four positioning blocks can be used to fit the four side walls of the microplate.

[0020] Push protection: When the baffle is pressed from a vertical state to a horizontal state, the first spring is used to slide the card strip into the card slot to fix the two baffles, so as to block the upper surface of the microplate during the pushing process. When the microplate moves to the detection position, the card strip will be detached from the card slot. At this time, the torsion spring is used to return the two baffles to a vertical state to prevent the baffles from blocking the detection position.

[0021] Push-pull cleaning: During the pushing process, one side of the two trigger top blocks can contact and push one side of the cleaning block. When the two baffles remain in a vertical state after resetting, the cleaning block returns to its original position under the rebound of the second spring and uses the electrostatic adsorption film in the cleaning cavity to adsorb and clean the dust on the surface of the baffle after sealing. When the tray is pulled out, the baffle can slide from the cleaning block to the outside to ensure the cleanliness of the sealing surface before and after the baffle is sealed.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] 1. During the push detection process, the large gear can be driven to rotate by pushing the tray, and the four positioning blocks can be moved inward synchronously by the cooperation of the guide groove and the shaft rod. When pushed to the detection position, the four positioning blocks can be used to fit and position the four side walls of the microplate, thereby ensuring the accuracy of the position of the microplate during the detection process, and thus ensuring the accuracy of the subsequent detection results of this equipment.

[0024] 2. During the push detection process, after placing the microplate, the two baffles can be manually pressed to fit and fix them to the upper surface of the microplate, so as to block the upper surface of the microplate during the pushing process to prevent the solution loaded inside the microplate from overflowing and mixing with other solutions during movement to cause contamination. At the same time, when pushed to the detection position, the two baffles can be automatically released to prevent the baffles from blocking the detection position, thereby ensuring the accuracy of subsequent detection results of the equipment.

[0025] 3. During the push detection process, the trigger top block can be used to accumulate force to push the cleaning block, so that when the two baffles are reset to a vertical state, the cleaning chamber can be used to clean the sealing surface of the baffle. At the same time, when the tray is pulled out for the next group of samples after the detection is completed, the reset cleaning block can be used to clean the sealing surface of the baffle again, ensuring the cleanliness of the sealing surface before and after the baffle is sealed, avoiding the solution from contacting and mixing with the dust on the sealing surface during shaking, ensuring the purity of the solution during detection, and then ensuring the accuracy of the subsequent test results of the equipment. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 It is a detection flow chart of the present invention;

[0027] Figure 2 It is a schematic diagram of the overall structure of the present invention;

[0028] Figure 3 It is a structural schematic diagram of the tray of the present invention in a pulled-out state;

[0029] Figure 4 It is a schematic diagram of the structure inside the detection chamber of the present invention;

[0030] Figure 5 It is a structural schematic diagram of the push positioning mechanism of the present invention;

[0031] Figure 6 It is a structural schematic diagram of the first part of the push protection mechanism of the present invention;

[0032] Figure 7 It is a structural schematic diagram of the second part of the push protection mechanism of the present invention;

[0033] Figure 8 It is a schematic structural diagram of the push-pull cleaning mechanism of the present invention.

[0034] Figure: 1, ELISA instrument; 2, detection chamber; 3, slide; 4, tray; 8, rack; 9, microplate; 10, detection component; 11, support plate; 5, push positioning mechanism; 501, main rod; 502, large gear; 503, small gear; 504, auxiliary rod; 505, guide groove; 506, shaft rod; 507, positioning block; 508, limit groove; 509, support groove; 510, support block; 6, push protection mechanism; 601, first fixed Fixed block; 602, rotating rod; 603, second fixed block; 604, baffle; 605, first spring; 606, support ring; 607, rotating groove; 608, clamping strip; 609, clamping groove; 610, torsion spring; 611, clamping column; 612, trigger top block; 7, push-pull cleaning mechanism; 701, sliding rod; 702, sliding block; 703, mounting groove; 704, mounting block; 705, cleaning block; 706, cleaning chamber; 707, second spring. DETAILED DESCRIPTION

[0035] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0036] For example, see Figure 1-8 The present invention provides a transfer factor detection device, comprising an ELISA instrument 1, a detection chamber 2, a microplate 9, and a detection component 10. The inner wall of the detection chamber 2 is symmetrically provided with a slide groove 3, and the inner wall of the slide groove 3 is slidably connected with a tray 4;

[0037] A push positioning mechanism 5 is provided at the bottom of the tray 4. Further, the push positioning mechanism 5 includes a main rod 501, and the top of the main rod 501 is rotatably connected to the bottom of the tray 4. A large gear 502 is fixedly connected to the bottom of the main rod 501, and a small gear 503 is meshed on one side of the large gear 502. A sub-rod 504 is fixedly connected to the top of the small gear 503, and the sub-rod 504 is rotatably connected to the bottom of the tray 4. A rack 8 is fixedly connected to the inner wall of one side of the detection chamber 2 corresponding to the small gear 503, and one side of the small gear 503 is meshed and matched with the tooth groove of the rack 8.

[0038] Further, after pushing the microplate 9 to the detection position, click the start detection button, and the microplate reader 1 will use the detection component 10 to make each well to be tested enter the light beam in turn for detection. The detection principle of this product is the existing technology product, so the specific principle of the detection will not be further described here;

[0039] In this embodiment, when preparing to put in the microplate 9, first pull the tray 4 out of the detection chamber 2, then carefully place the microplate 9 at the center of the tray 4, and then push the tray 4 back into the detection chamber 2, and connect it to the bottom of the tray 4 through the top of the main rod 501, and the bottom of the main rod 501 is fixedly connected with a large gear 502, and one side of the large gear 502 is meshed with a small gear 503, and at the same time, the top of the small gear 503 is fixedly connected with a sub-rod 504, and the sub-rod 504 is rotationally connected to the bottom of the tray 4, and the inner wall of one side of the detection chamber 2 is fixedly connected with the small gear 503, so that in the process of pushing the tray 4, the small gear 503 can be driven to rotate by the rack 8, and then the large gear 502 can be driven to rotate synchronously;

[0040] Then, guide grooves 505 are equidistantly penetrated through the top of the large gear 502, and the inner wall of the guide groove 505 is slidably connected with an axle rod 506, and the top of the axle rod 506 is fixedly connected with a positioning block 507, and at the same time, a limiting groove 508 is penetrated through the corresponding positioning block 507 at the top of the tray 4, and the outer walls on both sides of the positioning block 507 fit and slide with the inner wall of the limiting groove 508, so that during the rotation of the large gear 502, the positioning block 507 can be restricted by the limiting groove 508, so that the axle rod 506 slides from the inner wall of the farthest end of the guide groove 505 to the inner wall of the nearest end, so that the four positioning blocks 507 move inward synchronously during the pushing process, so that when it is pushed to the detection position, the four positioning blocks 507 can be used to fit and position the four side walls of the microplate 9, so as to ensure the accuracy of the position of the microplate 9 during the detection process;

[0041] Then, support grooves 509 are symmetrically opened through the inner wall of the limiting groove 508, and support blocks 510 are fixedly connected to the outer walls of the two sides of the positioning block 507 corresponding to the support grooves 509, and the outer wall of the support block 510 is fitted and slid with the inner wall of the support groove 509, so that the positioning block 507 can be supported by the cooperation of the support block 510 and the support groove 509, thereby ensuring the stability of the positioning block 507 during the movement;

[0042] As described above, during the push detection process, the large gear 502 can be driven to rotate by pushing the tray 4, and the four positioning blocks 507 can be synchronously moved inward by utilizing the cooperation between the guide groove 505 and the shaft rod 506. When pushed to the detection position, the four positioning blocks 507 can be used to fit and position the four side walls of the microplate 9, thereby ensuring the accuracy of the position of the microplate 9 during the detection process, and further ensuring the accuracy of the subsequent detection results of the device.

[0043] Embodiment 2:

[0044] On the basis of the above embodiment, a push protection mechanism 6 is symmetrically arranged on the top of the tray 4. Further, the push protection mechanism 6 includes a first fixed block 601, and the bottom of the first fixed block 601 is fixedly connected to the top of the tray 4. The first fixed block 601 is internally slidably connected to a rotating rod 602, and the outer wall of the other end of the rotating rod 602 is slidably connected to a second fixed block 603, the bottom of the second fixed block 603 is fixedly connected to the top of the tray 4, and the outer wall of the rotating rod 602 is fixedly connected to a baffle 604. The tops of the two baffles 604 are inclined, and the side surfaces of the two baffles 604 that are close to each other are sprayed with a nano coating. The outer wall of one end of the rotating rod 602 that is close to the second fixed block 603 is fitted with a first spring 605.

[0045] In this embodiment, during the push detection process, the rotating rod 602 is firstly connected to the inner sliding connection of the first fixed block 601, and the outer wall of the other end of the rotating rod 602 is slidably connected to the second fixed block 603, and the outer wall of the rotating rod 602 is fixedly connected to the baffle 604, and at the same time, the tops of the two baffles 604 are inclined, so that after the micro-well plate 9 is placed, the two baffles 604 can be manually pressed to fit with the upper surface of the micro-well plate 9, and then the outer wall of one end of the rotating rod 602 close to the second fixed block 603 is fitted with a first spring 605, and the other end of the first spring 605 is fixedly connected to a support ring 606, and the inner wall of the support ring 606 fits and rotates with the inner wall of the rotating groove 607, and at the same time, the rotating rod 602 closes to one end of the first fixed block 601 The outer wall of the end is fixedly connected with a clamping strip 608, and a clamping groove 609 is provided on a side wall of the first fixing block 601 away from the second fixing block 603 corresponding to the clamping strip 608, and the outer wall of the clamping strip 608 and the inner wall of the clamping groove 609 are fitted and slid, so that when the baffle 604 is pressed from a vertical state to a horizontal state, the clamping strip 608 can be driven to rotate to the position of the clamping groove 609, so that the clamping strip 608 and the clamping groove 609 are aligned, and at this time, the elastic force of the first spring 605 is used to drive the rotating rod 602 to slide, so that the clamping strip 608 slides into the clamping groove 609, and the two baffles 604 are automatically fixed, so as to block the upper surface of the microplate 9 during the pushing process, so as to prevent the solution loaded inside the microplate 9 from overflowing and mixing with other solutions during the movement to cause contamination;

[0046] The nano coating is sprayed on the side of the two baffles 604 that are close to each other, so that when the solution sways during the plugging process, the nano coating can be used to isolate the solution from adhering to the plugging surface, thereby preventing the solution content in the micropores from decreasing, thereby ensuring the accuracy of the subsequent detection results of the device;

[0047] Then, during the pushing process, when the microplate 9 approaches the detection position, one end of the rotating rod 602 conflicts with the inner wall of the detection chamber 2. At this time, the microplate 9 is moved to the detection position. At this time, the rotating rod 602 is restricted by the inner wall of the detection chamber 2 to drive the card strip 608 to disengage from the card slot 609. At this time, torsion springs 610 are equidistantly arranged on the outer wall of the rotating rod 602, and one end of the torsion spring 610 is overlapped on the outer wall of the rotating rod 602, and the other ends of the two torsion springs 610 are overlapped on the first A side wall of the first fixed block 601 close to the second fixed block 603 can use the torsion spring 610 to drive the rotating rod 602 to reverse and drive the two baffles 604 to rotate in the opposite direction. A side wall of the first fixed block 601 away from the second fixed block 603 is fixedly connected with a clamping column 611 corresponding to the clamping strip 608, so that the reverse rotation position of the baffle 604 can be limited, so that the two baffles 604 can accurately maintain a vertical state after resetting, and avoid the baffle 604 from blocking the detection position;

[0048] As described above, during the push detection process, after the microporous plate 9 is placed, the two baffles 604 can be manually pressed to fit and fix them to the upper surface of the microporous plate 9, so as to block the upper surface of the microporous plate 9 during the pushing process to prevent the solution loaded inside the microporous plate 9 from overflowing and mixing with other solutions during movement to cause contamination. At the same time, when pushed to the detection position, the two baffles 604 can be automatically released from their own fixation to prevent the baffles 604 from blocking the detection position, thereby ensuring the accuracy of subsequent detection results of the device.

[0049] Embodiment three:

[0050] On the basis of the above embodiment, the inner wall of the detection chamber 2 is symmetrically provided with a push-pull cleaning mechanism 7. Further, the push-pull cleaning mechanism 7 includes a slide bar 701, and the two ends of the slide bar 701 are fixedly connected to the inner wall of the detection chamber 2, the outer wall of the slide bar 701 is slidably connected with a slider 702, and a side wall of the slider 702 is provided with a mounting groove 703, the inner wall of the mounting groove 703 is slidably connected with a mounting block 704, and the bottom of the mounting block 704 is fixedly connected with a cleaning block 705, a cleaning cavity 706 is provided through one side wall of the cleaning block 705 corresponding to the baffle 604, and the inner wall of the cleaning cavity 706 is slidably fitted with the outer wall of the baffle 604, and the inner wall of the cleaning cavity 706 is provided with an electrostatic adsorption film;

[0051] In this embodiment, a slider 702 is slidably connected to the outer wall of the slide rod 701, and a mounting groove 703 is opened on one side wall of the slider 702, and a mounting block 704 is slidably connected to the inner wall of the mounting groove 703, and a cleaning block 705 is fixedly connected to the bottom of the mounting block 704, and a second spring 707 is provided on the outer wall of the slide rod 701, so that when the tray 4 is not pushed in, the two sliders 702 are located at the entrance of the detection chamber 2 under the elastic force of the second spring 707, and a side wall of the mounting block 704 is in a state of being ... The cleaning block 705 is installed and fixed to the inner wall of the entrance of the detection chamber 2 to prevent the cleaning block 705 from being separated from the slider 702. Then, when the two baffles 604 are pressed to a horizontal state and the tray 4 is pushed, the push-pull cleaning mechanism 7 is fixedly connected with the trigger top block 612 corresponding to the side of the two baffles 604 that is away from each other, so that one side of the two trigger top blocks 612 can contact and push one side of the cleaning block 705, so that the cleaning block 705 moves deep into the inner wall of the detection chamber 2 and compresses the second spring 707.

[0052] When the two baffles 604 remain in a vertical state after being reset, a cleaning cavity 706 is opened through a side wall of the cleaning block 705 corresponding to the baffle 604, and the inner wall of the cleaning cavity 706 slides in contact with the outer wall of the baffle 604, and the inner wall of the cleaning cavity 706 is provided with an electrostatic adsorption film, so that the cleaning block 705 slides from the two side surfaces of the baffle 604 under the rebound of the second spring 707, and uses the electrostatic adsorption film in the cleaning cavity 706 to adsorb and clean the dust on the surface of the baffle 604 after being blocked. Similarly, when the tray 4 is pulled out to test the next group of samples after the test is completed, the baffle 604 in a vertical state can slide from the cleaning block 705 to the outside, and the reset cleaning block 705 can be used to clean the blocking surface of the baffle 604 again, thereby ensuring the cleanliness of the blocking surface of the baffle 604 before and after blocking, and preventing the solution from contacting and mixing with the dust on the blocking surface when it sways during the blocking process, thereby ensuring the purity of the solution during the test.

[0053] At the same time, when cleaning the cleaning chamber 706, since the cleaning block 705 is normally located at the exit of the detection chamber 2, it is convenient to put your hand in. You can manually push the cleaning block 705 deep into the detection chamber 2 for a certain distance, and then press the slider 702 with your other hand. At this time, the cleaning block 705 can be removed, making it convenient to clean the cleaning chamber 706.

[0054] As described above, during the push detection process, the trigger top block 612 can be used to accumulate force to push the cleaning block 705, so that when the two baffles 604 are reset to a vertical state, the cleaning chamber 706 can be used to clean the sealing surface of the baffle 604. At the same time, when the tray 4 is pulled out after the detection is completed to detect the next group of samples, the reset cleaning block 705 can be used to clean the sealing surface of the baffle 604 again, ensuring the cleanliness of the sealing surface before and after the baffle 604 is sealed, avoiding the solution from contacting and mixing with the dust on the sealing surface during shaking, ensuring the purity of the solution during detection, and then ensuring the accuracy of subsequent detection results of the equipment.

[0055] The detection method of the transfer factor detection device is as follows: first, the tray 4 is pulled out from the detection chamber 2, and then the microplate 9 is carefully placed at the center of the tray 4, and the two baffles 604 are manually pressed to fit with the upper surface of the microplate 9, and then the tray 4 is pushed back into the detection chamber 2. In the process of pushing the tray 4, the small gear 503 can be driven to rotate by the rack 8, and then the large gear 502 is driven to rotate synchronously. In the process of the large gear 502 rotating, the positioning block 507 can be restricted by the limit groove 508, so that the shaft 506 slides from the inner wall of the farthest end of the guide groove 505 to the inner wall of the nearest end, so that the four positioning blocks 507 move inward synchronously during the pushing process, so that when it is pushed to the detection position, the four positioning blocks 507 can be used to fit and position the four side walls of the microplate 9;

[0056] Then, when the baffle 604 is pressed from the vertical state to the horizontal state, the clamping strip 608 can be driven to rotate to the position of the clamping slot 609, so that the clamping strip 608 is aligned with the clamping slot 609. At this time, the elastic force of the first spring 605 drives the rotating rod 602 to slide, so that the clamping strip 608 slides into the clamping slot 609, and the two baffles 604 are automatically fixed to block the upper surface of the microplate 9 during the pushing process;

[0057] When the microplate 9 approaches the detection position, one end of the rotating rod 602 contacts the inner wall of the detection chamber 2. At this time, the microplate 9 is pushed further to move to the detection position. At this time, the rotating rod 602 is restricted by the inner wall of the detection chamber 2 to drive the card strip 608 to disengage from the card slot 609. At this time, the torsion spring 610 is used to drive the rotating rod 602 to reverse and drive the two baffles 604 to rotate in the opposite direction. The card column 611 is used to limit the reverse rotation position of the baffle 604, so that the two baffles 604 can accurately maintain a vertical state after resetting, so as to prevent the baffle 604 from blocking the detection position.

[0058] Then, when the two baffles 604 are pressed to a horizontal state and the tray 4 is pushed, the trigger top block 612 is fixedly connected to the push-pull cleaning mechanism 7 through the side of the two baffles 604 that is away from each other, so that one side of the two trigger top blocks 612 can contact and push one side of the cleaning block 705, so that the cleaning block 705 moves deep into the inner wall of the detection chamber 2 and compresses the second spring 707. When the two baffles 604 remain in a vertical state after being reset, the cleaning block 705 is in the second Under the rebound of the spring 707, it slides from the two side surfaces of the baffle 604, and uses the electrostatic adsorption film in the cleaning chamber 706 to adsorb and clean the dust on the surface of the baffle 604 after sealing. Similarly, when the tray 4 is pulled out to test the next group of samples after the test is completed, the baffle 604 in a vertical state can slide from the cleaning block 705 to the outside, and the reset cleaning block 705 can be used to clean the sealing surface of the baffle 604 again, thereby ensuring the cleanliness of the sealing surface of the baffle 604 before and after sealing.

[0059] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A transfer factor detection device, It is characterized in that The invention comprises an enzyme marker (1), a detection chamber (2), a microplate (9), and a detection component (10); the inner wall of the detection chamber (2) is symmetrically provided with a slide groove (3), and the inner wall of the slide groove (3) is slidably connected with a tray (4); A push positioning mechanism (5) is provided at the bottom of the tray (4) to fit and fix the detection position of the microplate (9) during push detection; A push protection mechanism (6) is symmetrically arranged on the top of the tray (4) to block the upper surface of the microplate (9) during push detection; The inner wall of the detection chamber (2) is symmetrically provided with a push-pull cleaning mechanism (7) to clean the blocking surface before and after blocking during push detection.

2. A transfer factor detection device according to claim 1, It is characterized in that The push positioning mechanism (5) comprises a main rod (501), and the top of the main rod (501) is rotatably connected to the bottom of the tray (4), the bottom of the main rod (501) is fixedly connected to a large gear (502), and one side of the large gear (502) is meshed with a small gear (503), the top of the small gear (503) is fixedly connected to a secondary rod (504), and the secondary rod (504) is rotatably connected to the bottom of the tray (4), and the inner wall of one side of the detection chamber (2) is fixedly connected to a rack (8) corresponding to the small gear (503), and one side of the small gear (503) is meshed and matched with the tooth groove of the rack (8).

3. A transfer factor detection device according to claim 2, It is characterized in that The top of the large gear (502) is equidistantly penetrated with guide grooves (505), and the inner wall of the guide groove (505) is slidably connected with a shaft rod (506), and the top of the shaft rod (506) is fixedly connected with a positioning block (507), and the top of the tray (4) is penetrated with a limiting groove (508) corresponding to the positioning block (507), and the outer walls of both sides of the positioning block (507) are fitted and slid with the inner wall of the limiting groove (508), and the inner wall of the limiting groove (508) is symmetrically provided with supporting grooves (509), and the outer walls of both sides of the positioning block (507) are fixedly connected with supporting blocks (510) corresponding to the supporting grooves (509), and the outer wall of the support block (510) is fitted and slid with the inner wall of the supporting groove (509).

4. A transfer factor detection device according to claim 3, It is characterized in that The push protection mechanism (6) comprises a first fixed block (601), and the bottom of the first fixed block (601) is fixedly connected to the top of the tray (4); the first fixed block (601) is slidably connected to a rotating rod (602) inside, and the outer wall of the other end of the rotating rod (602) is slidably connected to a second fixed block (603); the bottom of the second fixed block (603) is fixedly connected to the top of the tray (4); the outer wall of the rotating rod (602) is fixedly connected to a baffle (604); the tops of the two baffles (604) are inclined; the side surfaces of the two baffles (604) that are close to each other are sprayed with a nano coating; and the outer wall of one end of the rotating rod (602) that is close to the second fixed block (603) is fitted with a first spring (605).

5. A transfer factor detection device according to claim 4, It is characterized in that One end of the first spring (605) is fixedly connected to a side wall of the second fixed block (603), and the other end of the first spring (605) is fixedly connected to a support ring (606); an outer wall of one end of the rotating rod (602) close to the second fixed block (603) is provided with a rotating groove (607) corresponding to the support ring (606), and the inner wall of the support ring (606) rotates in close contact with the inner wall of the rotating groove (607); an outer wall of one end of the rotating rod (602) close to the first fixed block (601) is fixedly connected to a clamping strip (608), and a side wall of the first fixed block (601) away from the second fixed block (603) is provided with a clamping slot (609) corresponding to the clamping strip (608), and the outer wall of the clamping strip (608) slides in close contact with the inner wall of the clamping slot (609).

6. A transfer factor detection device according to claim 5, It is characterized in that Torsion springs (610) are arranged at equal distances on the outer wall of the rotating rod (602), and one end of the torsion spring (610) is overlapped on the outer wall of the rotating rod (602), and the other ends of the two torsion springs (610) are overlapped on the side wall of the first fixed block (601) and the second fixed block (603) respectively, and a side wall of the first fixed block (601) away from the second fixed block (603) is fixedly connected to a clamping column (611) corresponding to the clamping strip (608), and a side surface of the two baffles (604) away from each other is fixedly connected to a trigger top block (612) corresponding to the push-pull cleaning mechanism (7).

7. A transfer factor detection device according to claim 6, It is characterized in that The push-pull cleaning mechanism (7) comprises a sliding rod (701), and the two ends of the sliding rod (701) are fixedly connected to the inner wall of the detection chamber (2); the outer wall of the sliding rod (701) is slidably connected to a slider (702), and a side wall of the slider (702) is provided with a mounting groove (703); the inner wall of the mounting groove (703) is slidably connected to a mounting block (704), and the bottom of the mounting block (704) is fixedly connected to a cleaning block (705); a cleaning cavity (706) is formed through one side wall of the cleaning block (705) corresponding to the baffle (604), and the inner wall of the cleaning cavity (706) is slidably fitted with the outer wall of the baffle (604), and the inner wall of the cleaning cavity (706) is provided with an electrostatic adsorption film.

8. A transfer factor detection device according to claim 7, It is characterized in that A second spring (707) is fitted on the outer wall of the slide bar (701), one end of the second spring (707) is fixedly connected to the inner wall of the detection chamber (2), and the other end of the second spring (707) is fixedly connected to a side wall of the slide block (702).

9. A transfer factor detection device according to claim 1, It is characterized in that A support plate (11) is fixedly mounted on one side of the tray (4), and a handle is fixedly mounted on one side of the support plate (11).

10. A detection method of a transfer factor detection device according to claim 8, It is characterized in that The following steps are involved: S1: Pushing and positioning, pulling the tray (4) out of the detection chamber (2), placing the microplate (9) at the center of the tray (4), manually pressing the two baffles (604) to fit the upper surface of the microplate (9), and pushing the tray (4) back into the detection chamber (2). At this time, the four positioning blocks (507) move inward synchronously during the pushing process, so that when the tray is pushed to the detection position, the four side walls of the microplate (9) are fitted and positioned using the four positioning blocks (507); S2: Push protection, when the baffle (604) is pressed from the vertical state to the horizontal state, the first spring (605) is used to make the card strip (608) slide into the card slot (609), so as to fix the two baffles (604) so ​​as to block the upper surface of the microplate (9) during the pushing process. When the microplate (9) moves to the detection position, the card strip (608) will be disengaged from the card slot (609), and the torsion spring (610) is used to make the two baffles (604) return to the vertical state, so as to prevent the baffles (604) from blocking the detection position; S3: Push-pull cleaning. During the pushing process, one side of the two trigger top blocks (612) contacts and pushes against one side of the cleaning block (705). When the two baffles (604) remain in a vertical state after being reset, the cleaning block (705) returns to its original position under the rebound of the second spring (707) and uses the electrostatic adsorption film in the cleaning cavity (706) to adsorb and clean the dust on the surface of the baffle (604) after being blocked. When the tray (4) is pulled out, the baffle (604) slides from the cleaning block (705) to the outside to ensure the cleanliness of the blocking surface of the baffle (604) before and after blocking.

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