Application of hydrolat / extract in preparation of anti-allergic and anti-inflammatory drugs and cosmetics

Abstract

The invention discloses application of hydrolat / extract in preparation of anti-allergic and anti-inflammatory drugs and cosmetics. Belongs to the technical field of natural extracts. The hydrolat / extract is prepared from the following raw materials: rose flowers, peach blossoms and calendula flowers, wherein the mass ratio of the rose flowers to the peach blossoms to the calendula flowers is (30-45): (22-42): (17-43). The pure natural anti-inflammatory and anti-allergic hydrolat / extract provided by the invention does not contain chemical preservatives, ensures the pure natural quality, is safe and environment-friendly, and can be used for producing cosmetics, foods, pet foods, medicines and the like with anti-inflammatory and anti-allergic functions.

Description

Technical Field

[0001] The invention relates to the technical field of natural extracts, and more particularly to the application of hydrosols / extracts in the preparation of anti-allergic and anti-inflammatory drugs and cosmetics. Background Art

[0002] Allergic diseases can occur at all ages from newborns to the elderly, and often have a clear genetic tendency. Among allergic diseases, immediate allergic reactions are more common, and their main types include skin allergic reactions, respiratory allergic reactions, digestive tract allergic reactions and anaphylactic shock. Immediate allergic reactions are mediated by IgE. Antigens (allergens) and specific IgE (combined with tissue mast cells and blood basophils) trigger the release of inflammatory mediators, including existing inflammatory mediators (such as histamine, proteases, chemokines) and newly synthesized other mediators (such as prostaglandins, leukotrienes, platelet-activating factor, interleukins). These inflammatory mediators can lead to vasodilation, increased capillary permeability, hypersecretion of mucus, contraction of smooth muscles, and infiltration of eosinophils, helper T cells (TH2) and other inflammatory cells in tissues.

[0003] At present, anti-allergy mainly uses antihistamines, steroid nasal sprays, anti-allergy ointments, diphenhydramine creams, moisturizers, saline flushing, cold compresses, etc. There is a lack of natural plant anti-inflammatory and anti-allergic raw materials and products.

[0004] In summary, how to provide a natural anti-inflammatory and anti-allergic substance is a problem that technical personnel in this field urgently need to solve. Summary of the invention

[0005] In view of this, the present invention provides the use of hydrosols / extracts in the preparation of anti-allergic and anti-inflammatory drugs and cosmetics.

[0006] In order to achieve the above object, the present invention adopts the following technical solution:

[0007] The invention discloses an application of a hydrosol / extract in the preparation of anti-allergic and anti-inflammatory drugs and cosmetics. The raw materials of the hydrosol / extract include rose, peach and marigold, and the mass ratio of the three is (30-45): (22-42): (17-43).

[0008] Furthermore, the raw materials of the hydrosol / extract include rose, peach blossom and calendula, and the mass ratio of the three is 35:22:43.

[0009] Further, the preparation method of the hydrosol / extract is as follows:

[0010] (1) Soaking the raw materials in water;

[0011] (2) Take out the raw materials and put them into the steaming sheet, pour the water after soaking into the bottom pot of the hydrosol machine, and distill at normal pressure;

[0012] (3) collecting the distillate, and stopping the distillation when the collected distillate reaches 35-45% of the amount of water added to obtain the pure dew;

[0013] (4) filtering the remaining material in the bottom pot of the hydrosol machine to obtain a filtrate;

[0014] (5) The filtrate is concentrated under reduced pressure and spray-dried to obtain an extract.

[0015] Furthermore, in step (1), the mass volume ratio of the raw material to water is 1: (15-18) g / ml, and the soaking time is 12-16 hours.

[0016] Furthermore, in step (2), the distillation temperature is 100-110°C.

[0017] Furthermore, in the step (5), the parameters for vacuum concentration are: -0.1 MPa, temperature 60°C, and vacuum concentration to a relative density of 1.1.

[0018] Furthermore, in step (5), the spray drying parameters are: 125° C., feed rate 30 mL / min, and air extraction volume 100%.

[0019] It can be seen from the above technical solution that, compared with the prior art, the beneficial effects achieved by the present invention are:

[0020] The present invention provides a pure natural anti-inflammatory and anti-allergic hydrosol / extract, which does not contain chemical preservatives, ensures pure natural quality, is safe and environmentally friendly, and can be used to produce cosmetics, food, pet food, medicines, etc. with anti-inflammatory and anti-allergic functions. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without paying creative work.

[0022] Figure 1 The accompanying drawings are raw materials and product diagrams of the present invention. DETAILED DESCRIPTION

[0023] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0024] The drugs required for the present invention are conventional experimental drugs, which are purchased from commercial channels; the experimental methods not mentioned are conventional experimental methods and will not be described in detail here.

[0025] Example 1

[0026] Weigh 30 parts of dried roses, 42 parts of peach blossoms, and 28 parts of marigolds (by weight), wash with clean water, put them in a container, add 18 times the amount of water, soak for 12 hours, and after soaking, remove the flowers and put them in a steaming sheet, pour the soaked water into the bottom pot of a hydrosol machine, start steam heating and atmospheric distillation, collect the distillate, and stop distillation when the collected distillate reaches 35% of the mass of the added water, and obtain the hydrosol for standby use. The heating temperature is 100°C. Filter the residue in the bottom pot of the hydrosol machine to obtain the filtrate, concentrate under reduced pressure (-0.1mpa, temperature is 60°C, relative density is 1.1), and spray dry (125°C, feed rate is 30mL / min, and air extraction volume is 100%) to obtain an extract.

[0027] Example 2

[0028] Weigh 35 parts of dried roses, 22 parts of peach blossoms, and 43 parts of marigolds (by weight), wash with clean water, put them in a container, add 18 times the amount of water, soak for 12 hours, and after soaking, remove the flowers and put them in a steaming sheet, pour the soaked water into the bottom pot of a hydrosol machine, start steam heating and atmospheric distillation, collect the distillate, and stop distillation when the collected distillate reaches 35% of the mass of the added water, and obtain the hydrosol for standby use. The heating temperature is 100°C. Filter the residue in the bottom pot of the hydrosol machine to obtain the filtrate, concentrate under reduced pressure (-0.1mpa, temperature is 60°C, relative density is 1.1), and spray dry (125°C, feed rate is 30mL / min, and air extraction volume is 100%) to obtain the extract.

[0029] Example 3

[0030] Weigh 45 parts of dried roses, 38 parts of peach blossoms, and 17 parts of marigolds (by weight), wash with clean water, put them in a container, add 18 times the amount of water, soak for 12 hours, and after soaking, remove the flowers and put them in a steaming sheet, pour the soaked water into the bottom pot of a hydrosol machine, start steam heating and atmospheric distillation, collect the distillate, and stop distillation when the collected distillate reaches 35% of the mass of the added water, and obtain the hydrosol for standby use. The heating temperature is 100°C. Filter the residue in the bottom pot of the hydrosol machine to obtain the filtrate, concentrate under reduced pressure (-0.1mpa, temperature is 60°C, relative density is 1.1), and spray dry (125°C, feed rate is 30mL / min, and air extraction volume is 100%) to obtain an extract.

[0031] Comparative Example 1

[0032] Weigh 78 parts of dried roses, 14 parts of peach blossoms, and 8 parts of marigolds (by weight), wash with clean water, put them in a container, add 18 times the amount of water, soak for 12 hours, take out the flowers after soaking, put them in a steaming sheet, pour the soaked water into the bottom pot of a hydrosol machine, start steam heating and atmospheric distillation, collect the distillate, and stop distillation when the collected distillate reaches 35% of the mass of the added water, and obtain the hydrosol for standby use. The heating temperature is 100°C.

[0033] Comparative Example 2

[0034] Weigh 100 parts of dried roses, wash with water, put them in a container, add 18 times the amount of water, soak for 12 hours, take out the roses after soaking, put them in a steamer, pour the soaked water into the bottom pot of the hydrosol machine, start water steam heating and atmospheric distillation, collect the distillate, and stop distillation when the collected distillate reaches 35% of the mass of the added water, and obtain rose hydrosol for standby use. The heating temperature is 100°C.

[0035] Comparative Example 3

[0036] Weigh 100 parts of dried peach buds, wash with water, put them in a container, add 18 times the amount of water, soak for 12 hours, take out the peach buds after soaking, put them in a steaming sheet, pour the soaked water into the bottom pot of the hydrosol machine, start water steam heating and atmospheric distillation, collect the distillate, and stop distillation when the collected distillate reaches 35% of the mass of the added water, and obtain peach blossom hydrosol for standby use. The heating temperature is 100°C.

[0037] Comparative Example 4

[0038] Weigh 100 parts of dried marigolds, wash with water, put into a container, add 18 times the amount of water, soak for 12 hours, take out the marigolds after soaking, put them into a steamer, pour the soaked water into the bottom pot of a hydrosol machine, start steam heating and atmospheric distillation, collect the distillate, and stop distillation when the collected distillate reaches 35% of the mass of the added water, and obtain the marigold hydrosol for standby use. The heating temperature is 100°C.

[0039] Experiment 1

[0040] Anti-allergic effect experiment

[0041] SPF BN (Brown-Norway) rats, male, weight (200±10) g. Adaptive feeding for 7 days with standard diet and free access to water. Ambient temperature: 24±1°C, humidity: 55±10%.

[0042] The allergic reaction model was constructed by ovalbumin (OVA) injection. OVA was purchased from Sigma, item number A5503, with a purity of >98%. 1g of OVA was dissolved in 100mL of 0.1M PBS to obtain an OVA solution. Construction method: 1mL of OVA solution was injected intraperitoneally on the 1st, 7th, and 14th days after the start of the experiment, and 2mL of OVA solution was injected intraperitoneally on the 21st day. The score was statistically calculated 7 days after the last intraperitoneal OVA injection, blood was collected from the tail vein, and the animals were killed.

[0043] Allergy symptom scoring: Allergy symptom scoring was performed before the mice were killed. The specific scoring criteria are as follows:

[0044] 0 points: no symptoms;

[0045] 1 point: scratching the face;

[0046] 2 points: facial redness and swelling, shortness of breath, and mental depression;

[0047] 3 points: tearing, rash around the mouth and tail, asthma, and difficulty breathing;

[0048] 4 points: inactivity, muscle contraction, spasm;

[0049] 5 points: Death, shock.

[0050] The scores of mice in each group were counted and the average value was calculated.

[0051] A total of 12 groups were set up, with 7 mice in each group. The gavage samples were 5 mL of the pure dew of each embodiment / comparative example / 300 mg / kg of the extract (if the extract was gavaged, the extract was dissolved in physiological saline and gavaged after the dosage was calculated according to the body weight). The specific treatment methods of each group were as follows:

[0052] ① Blank control group: 1 mL of 0.1 M PBS was injected intraperitoneally on the 1st, 7th, 14th and 21st days after the start of the experiment, and 5 mL of normal saline was gavaged daily;

[0053] ② Allergic reaction model group: The model was constructed according to the above model construction method, and 5 mL of normal saline was gavaged daily;

[0054] ③ Example 1 hydrosol group: the model was constructed according to the above model construction method, and 5 mL of the hydrosol of Example 1 was intragastrically administered daily;

[0055] ④ Example 2 hydrosol group: The model was constructed according to the above model construction method, and 5 mL of the hydrosol of Example 2 was gavaged daily;

[0056] ⑤ Example 3 hydrosol group: the model was constructed according to the above model construction method, and 5 mL of the hydrosol of Example 3 was gavaged daily;

[0057] ⑥ The extract group of Example 1: the model was constructed according to the above model construction method, and 5 mL of the extract of Example 1 was gavaged daily;

[0058] ⑦Example 2 extract group: the model was constructed according to the above model construction method, and 5 mL of the extract of Example 2 was gavaged daily;

[0059] ⑧Example 3 extract group: the model was constructed according to the above model construction method, and 5 mL of the extract of Example 3 was gavaged daily;

[0060] ⑨ Comparative Example 1 Hydrosol Group: The model was constructed according to the aforementioned model construction method, and 5 mL of the hydrosol of Comparative Example 1 was intragastrically administered daily;

[0061] ⑩Comparative Example 2 Hydrosol Group: The model was constructed according to the aforementioned model construction method, and 5 mL of the hydrosol of Comparative Example 2 was intragastrically administered daily;

[0062] Comparative Example 3 Hydrosol Group: The model was constructed according to the above model construction method, and 5 mL of the hydrosol of Comparative Example 3 was intragastrically administered daily;

[0063] Comparative Example 4 Hydrosol Group: The model was constructed referring to the aforementioned model construction method, and 5 mL of the hydrosol of Comparative Example 4 was administered intragastrically daily.

[0064] Detection indicators: ELISA kit detects IgE, IgG, IL-4, IL-13, IL-12, IFN-γ, TNF-α and histamine (HIS). The detection steps are carried out according to the instructions.

[0065] The results are shown in Table 1.

[0066] Table 1 Anti-inflammatory and anti-allergic test results

[0067]

[0068] Table 1 Appendix

[0069]

[0070]

[0071] According to Table 1, it can be seen that the hydrosol groups of Examples 1 to 3, the extract groups of Examples 1 to 3, and the hydrosol groups of Comparative Examples 1 to 4 all have the effect of relieving allergic symptoms, and the hydrosol groups of Examples 1 to 3 and the extract groups of Examples 1 to 3 are better than the hydrosol groups of Comparative Examples 1 to 4 in relieving allergies, and the hydrosol groups of Example 2 and the extract groups of Example 2 are more effective in relieving allergies. The IgE and IgG contents of the allergic reaction model group are much higher than those of the blank control group, indicating that the mice in the allergic reaction model group have allergic reactions; the IgE and IgG contents of the hydrosol groups of Examples 1 to 3, the extract groups of Examples 1 to 3, and the hydrosols of Comparative Examples 1 to 4 are significantly reduced, indicating that the samples of the examples and the comparative examples all have a certain anti-allergic effect, and the effects of the samples of the examples are better than those of the comparative examples, and the hydrosol groups of Example 2 and the extract groups of Example 2 are better in relieving allergies. HIS, IL-4, TNF-α, IFN-γ, and IL-13 are serum inflammatory factors of allergic reactions, which increased significantly in the allergic reaction model group; after oral administration of the product of the embodiment group, the serum inflammatory factors of mice decreased significantly, which was a significant improvement compared with the control group, and the anti-allergic effects of the hydrosol group of embodiment 2 and the extract group of embodiment 2 were the best. IL-12 is an anti-inflammatory factor, which decreased significantly in the allergic reaction model group; after oral administration of the product of the embodiment group, IL-12 was significantly downregulated, which was a significant improvement compared with the control group, and the anti-allergic effects of the hydrosol group of embodiment 2 and the extract group of embodiment 2 were the best.

[0072] Through the study of the raw material ratio and preparation process of hydrosol / extract, the influence of each ratio and process on the anti-allergic effect of hydrosol / extract was investigated, and the appropriate ratio and preparation process were determined as follows: weigh 35 parts of dried rose, 22 parts of peach blossom, and 43 parts of marigold (by weight), wash with clean water, put them in a container, add 18 times the amount of water, soak for 12 hours, after soaking, remove the flowers, put them in the steaming sheet, pour the soaked water into the bottom pot of the hydrosol machine, start water steam heating atmospheric distillation, collect the distillate, and stop distillation when the collected distillate reaches 35% of the mass of the added water, and obtain the hydrosol for standby use. Among them, the heating temperature is 100℃. The residue in the bottom pot of the hydrosol machine is filtered to obtain the filtrate, which is concentrated under reduced pressure (-0.1mpa, temperature is 60℃, relative density is 1.1), spray dried (125℃, feed rate is 30mL / min, and air extraction volume is 100%) to obtain the extract. Under this condition, the hydrosol / extract has good anti-allergic effect, providing new raw materials and new products for the research and development of anti-allergic, anti-inflammatory drugs and cosmetics.

[0073] Application Example 1

[0074] Allergic rhinitis

[0075] The modeling method and drug administration method were the same as those in Experiment 1.

[0076] After nasal provocation, rats showed nasal scratching, sneezing, and rhinorrhoea. The scoring criteria are as follows:

[0077] Scratching the nose 1 to 5 times was 1 point, 6 to 15 times was 2 points, and >15 times was 3 points;

[0078] Sneezing 1 to 3 times is 1 point, 4 to 10 times is 2 points, and >10 times is 3 points;

[0079] Nasal overflow: 1 point if it flows to the anterior nasal aperture, 2 points if it flows out of the anterior nasal aperture, and 3 points if it fills the entire face.

[0080] The scores of rats in each group were counted and the average value was calculated.

[0081] The results are shown in Table 2.

[0082] Table 2 Effects on the behavior of rats with rhinitis

[0083] Group Nose scratching action sneeze Rhinorrhoea Blank control group 0.63±0.17 0.38±0.08 0.22±0.14 Allergic reaction model group 2.86±0.21 2.60±0.16 2.62±0.31 Embodiment 1 Hydrosol Group 1.44±0.53 1.20±0.61 1.37±0.62 Embodiment 2 Hydrosol Group 0.75±0.17 0.59±0.18 0.43±0.17 Embodiment 3 Hydrosol Group 0.94±0.26 0.87±0.44 0.72±0.15 Example 1 Extract Group 1.07±0.11 0.98±0.31 0.93±0.50 Example 2 Extract Group 0.69±0.20 0.48±0.10 0.29±0.19 Example 3 Extract Group 0.83±0.32 0.66±0.27 0.58±0.22 Comparative Example 1 Hydrosol Group 1.69±0.35 1.38±0.53 1.46±0.28 Comparative Example 2 Hydrosol Group 1.98±0.42 1.88±0.38 1.89±0.16 Comparative Example 3 Hydrosol Group 1.87±0.47 1.69±0.45 1.72±0.48 Comparative Example 4 Hydrosol Group 2.34±0.66 2.27±0.55 2.30±0.76

[0084] As can be seen from Table 2, compared with the allergic reaction model group, the behavioral scores of allergic rhinitis in rats in the hydrosol groups of Examples 1 to 3, the extract groups of Examples 1 to 3 and the hydrosol groups of Comparative Examples 1 to 4 were all reduced, especially the hydrosol group of Example 2 and the extract group of Example 2 had more significant effects on the behavior of allergic rhinitis in rats, with better effects.

[0085] Application Example 2

[0086] Anti-skin allergy

[0087] Antipruritic experiment: 120 healthy Kunming mice, half male and half female, weighing 18-22 grams. Randomly divided into 12 groups, 10 mice in each group. 8% sodium sulfide alcohol solution was used to remove hair on the back (2cm×3cm), and the test drug was applied to the skin of the depilated area once a day for 7 consecutive days. The allergic reaction model group and the blank control group were coated with an equal amount of normal saline. 40 minutes after the last administration, normal saline was injected subcutaneously at the back of the neck. The number of lickings in each group within 10 minutes was recorded (if the licking continued until a brief pause, it was counted as 1 licking).

[0088] Determination of skin histamine concentration: After the antipruritic experiment, the mice were immediately killed by cervical dislocation, and the sensitized skin of the same area (1.5 cm×2 cm) was cut off and prepared into tissue homogenate; the histamine concentration of the tissue homogenate was determined by enzyme-linked immunosorbent assay.

[0089] The results are shown in Table 3.

[0090] Table 3 Anti-skin allergy effect

[0091] Group Number of licks within 10 minutes Histamine concentration (μg / gprotein) Blank control group - 2.64±0.13 Allergic reaction model group 46±1 5.63±0.22 Embodiment 1 Hydrosol Group 26±4 3.92±0.34 Embodiment 2 Hydrosol Group 15±2 2.99±0.57 Embodiment 3 Hydrosol Group 21±1 3.32±0.28 Example 1 Extract Group 24±3 3.56±0.38 Example 2 Extract Group 13±2 2.88±0.14 Example 3 Extract Group 18±3 3.07±0.42 Comparative Example 1 Hydrosol Group 29±2 4.02±0.26 Comparative Example 2 Hydrosol Group 40±5 4.86±0.17 Comparative Example 3 Hydrosol Group 33±3 4.13±0.35 Comparative Example 4 Hydrosol Group 36±4 4.41±0.24

[0092] It can be seen from Table 3 that compared with the allergic reaction model group, the hydrosol groups of Examples 1 to 3, the extract groups of Examples 1 to 3 and the hydrosol groups of Comparative Examples 1 to 4 all have anti-skin allergy effects, especially the hydrosol group of Example 2 and the extract group of Example 2 have more significant and better anti-skin allergy effects.

[0093] The various embodiments in this specification are described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts between the various embodiments can be referenced to each other.

[0094] The above description of the disclosed embodiments enables one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. Application of hydrosol / extract in the preparation of anti-allergic, anti-inflammatory drugs and cosmetics, It is characterized in that The raw materials of the hydrosol / extract include rose flower, peach flower and marigold, and the mass ratio of the three is (30-45):(22-42):(17-43).

2. The use according to claim 1, It is characterized in that The raw materials of the hydrosol / extract include rose, peach blossom and calendula, and the mass ratio of the three is 35:22:

43.

3. The use according to claim 1, It is characterized in that The preparation method of hydrosol / extract is as follows: (1) Soaking the raw materials in water; (2) Take out the raw materials and put them into the steaming sheet, pour the water after soaking into the bottom pot of the hydrosol machine, and distill at normal pressure; (3) collecting the distillate, and stopping the distillation when the collected distillate reaches 35-45% of the amount of water added to obtain the pure dew; (4) filtering the remaining material in the bottom pot of the hydrosol machine to obtain a filtrate; (5) The filtrate is concentrated under reduced pressure and spray-dried to obtain an extract.

4. The use according to claim 3, It is characterized in that In the step (1), the mass volume ratio of the raw material to water is 1: (15-18) g / ml, and the soaking time is 12-16 hours.

5. The use according to claim 3, It is characterized in that In the step (2), the distillation temperature is 100-110°C.

6. The use according to claim 3, It is characterized in that In the step (5), the parameters for reduced pressure concentration are: -0.1 MPa, temperature 60°C, and reduced pressure concentration to a relative density of 1.

1.

7. The application as claimed in claim 3, It is characterized in that In the step (5), the spray drying parameters are: 125° C., feed rate 30 mL / min, and air extraction volume 100%.

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