Traditional Chinese medicine formula for treating gastric cancer as well as preparation method and application of traditional Chinese medicine formula

Abstract

The invention discloses a traditional Chinese medicine formula for treating gastric cancer as well as a preparation method and application thereof. The traditional Chinese medicine formula comprises the following raw material medicines in parts by weight: 3-9 parts of radix angelicae, 3-9 parts of coptis chinensis, 3-9 parts of ginseng, 15-30 parts of salvia chinensis, 10-15 parts of akebia fruit, 9-15 parts of polygonum cuspidatum and 3-9 parts of lindera aggregata. The traditional Chinese medicine formula disclosed by the invention is researched and developed on the basis of triple jiao system theoretical arrangement research and laboratory research on the basis of gastric cancer pathogenesis, has the functions of regulating qi and harmonizing stomach, eliminating stagnation and relieving pain, dispersing blood stasis and detoxifying, and dredging triple jiao, and has a good treatment effect on gastric cancer. The general treatment principle is to strengthen body resistance and eliminate pathogenic factors, regulate qi and harmonize stomach, dissipate stagnation and stop pain, and disperse blood stasis and detoxify. Experiments prove that the traditional Chinese medicine composition disclosed by the invention has a good anti-gastric cancer effect and low toxicity, is a great innovation for treating gastric cancer, and has remarkable clinical significance.

Description

Technical Field

[0001] The invention relates to a Chinese medicine formula for treating gastric cancer, a preparation method and application thereof, and belongs to the technical field of Chinese medicines. Background Art

[0002] Gastric cancer refers to a malignant tumor originating from the stomach wall. It is the fifth most common cancer in the world and the third leading cause of cancer-related death. According to Globocan 2022 data, there are 691,000 new cases of gastric cancer worldwide, with the highest incidence in Asia, accounting for 71.4% of global cases. China has about 359,000 new cases of gastric cancer and about 260,000 deaths each year, ranking fifth and third in the incidence and mortality of malignant tumors in China, respectively. Gastric cancer has significant geographical distribution differences. The incidence rate is high in East Asian countries, and China is a high-incidence area for gastric cancer, with both the incidence and mortality rates far higher than the world average. This may be related to diet, Helicobacter pylori infection, and genetic factors. Gastric cancer treatment focuses on surgery, chemotherapy, targeted therapy, and immunotherapy. With the continuous development of molecular biology, genetics, and precision medicine, gastric cancer treatment is becoming more and more diversified, emphasizing individualized and multidisciplinary comprehensive treatment.

[0003] Traditional Chinese medicine has been increasingly valued in the comprehensive treatment of gastric cancer, especially in terms of adjuvant chemotherapy, improving symptoms, improving quality of life, and reducing recurrence. In recent years, with the deepening of basic research on traditional Chinese medicine and the advancement of modern medical technology, the role of traditional Chinese medicine in the treatment of gastric cancer has also had a clearer scientific basis. Therefore, strengthening the research and development of new traditional Chinese medicine prescriptions for gastric cancer and further improving the treatment effect are of great significance for improving the level and ability of clinical prevention and treatment and reducing the mortality rate of gastric cancer patients. Summary of the invention

[0004] In view of the deficiencies in the prior art, the purpose of the present invention is to provide a Chinese medicine formula for treating gastric cancer, a preparation method and an application thereof, so as to play a role in the clinical diagnosis, treatment and prevention of gastric cancer, reduce the mortality rate of gastric cancer patients, prolong survival time and improve the quality of life of patients.

[0005] In order to achieve the above-mentioned purpose, the technical solution of the present invention is, on the one hand, to provide a Chinese medicine formula for treating gastric cancer, comprising the following raw materials in parts by weight: 3-9 parts of Angelica dahurica, 3-9 parts of Coptis chinensis, 3-9 parts of Panax ginseng, 15-30 parts of Lithospermum officinale, 10-15 parts of Semen Preparata, 9-15 parts of Polygonum cuspidatum, and 3-9 parts of Linderae strychnifolia.

[0006] Preferably, the Chinese medicine formula for treating gastric cancer comprises the following raw materials in parts by weight: 3 parts of Angelica dahurica, 3 parts of Coptis chinensis, 3 parts of Panax ginseng, 15 parts of Lithospermum officinale, 10 parts of Semen Preparata, 9 parts of Polygonum cuspidatum, and 3 parts of Linderae scutellariae.

[0007] On the other hand, the technical scheme of the present invention is to provide a method for preparing the traditional Chinese medicine formula for treating gastric cancer, wherein each raw material drug is extracted with ethanol, then dried, and then the dried extracts are mixed. Specifically, the method comprises the following steps:

[0008] (1) Wash and chop Angelica dahurica, Coptis chinensis, Panax ginseng, Lithospermum officinale, Fructus cyrtonemae, Polygonum cuspidatum, and Linderae sibiricum respectively, and grind them into coarse powder;

[0009] (2) adding ethanol solution to each raw material drug respectively, soaking at room temperature in the dark, then adding it to a reflux bottle for extraction, boiling, filtering, taking the filtrate, recovering the drug residue, repeating the above extraction steps, and taking the filtrate;

[0010] (3) The two filtrates of each raw material drug are mixed, respectively, placed on a rotary evaporator to remove ethanol, then evaporated in a water bath, placed in a refrigerator for freezing, and finally freeze-dried and crushed into powder;

[0011] (4) Mix the powders of the raw materials to obtain the product.

[0012] Furthermore, the volume concentration of the ethanol solution in step (2) is 70-75%, and the amount used is 8-10 times the volume of the raw material drug.

[0013] Furthermore, the soaking time in step (2) is 24-48 hours.

[0014] Furthermore, the reflux temperature in step (2) is 90-100°C.

[0015] Furthermore, the evaporation temperature of the rotary evaporator in step (3) is 42-50°C.

[0016] Furthermore, in step (3), the water bath temperature is 70-80°C.

[0017] On the other hand, the technical solution of the present invention is to provide an application of the traditional Chinese medicine formula in the preparation of drugs for treating gastric cancer.

[0018] Pharmacological effects of each raw material drug of the present invention:

[0019] Angelica dahurica is pungent and warm in nature and flavor; it enters the stomach, large intestine, and lung meridians. Its main functions are to relieve the cold, dispel wind and relieve pain, clear the nasal passages, dry dampness and stop leukorrhea, and reduce swelling and discharge pus.

[0020] Coptis chinensis, bitter and cold in nature; enters the heart, spleen, stomach, liver, gallbladder, and large intestine meridians. Main effects: clearing away heat and dampness, purging fire and detoxifying.

[0021] Ginseng is sweet, slightly bitter and slightly warm in nature and flavor; it enters the spleen, lung, heart and kidney meridians. Main effects: greatly replenishing vital energy, restoring pulse and strengthening deficiency, nourishing spleen and lungs, promoting body fluid and nourishing blood, calming the mind and improving intelligence.

[0022] Lithospermum officinale, pungent, bitter, slightly cold in nature; enters the liver and spleen meridians. Main effects: promoting blood circulation and removing blood stasis, clearing away heat and dampness, dispersing nodules and reducing swelling.

[0023] Precipitous seeds are bitter and cold in nature and enter the liver, gallbladder, stomach and bladder meridians. Main effects: soothing the liver and regulating qi, promoting blood circulation and relieving pain, dispersing stagnation and promoting urination.

[0024] Polygonum cuspidatum, slightly bitter and cold in nature; enters the liver, gallbladder, and lung meridians. Main effects: dispelling dampness and jaundice, clearing away heat and detoxifying, dispersing blood stasis and relieving pain, relieving cough and reducing phlegm.

[0025] Linderae odoratum, pungent and warm in nature; enters the lung, spleen, kidney and bladder meridians. Main effects: promoting qi and relieving pain, warming the kidney and dispersing cold.

[0026] Traditional Chinese medicine believes that gastric cancer belongs to the category of cancer, and is mostly caused by factors such as improper diet, emotional imbalance, spleen and stomach weakness, internal blood stasis, phlegm and dampness, cold evil invading the stomach, and damp heat in the spleen and stomach. The basic pathogenesis is the accumulation of qi stagnation, phlegm and dampness, and blood stasis in the stomach, which leads to stomach disharmony. The body is deficient in vital energy and invades with evil toxins, which leads to the disease over time.

[0027] The inventor believes that from the perspective of the three energizers, it can be found that the occurrence of gastric cancer is closely related to pathological mechanisms such as obstruction of the middle energizer, stagnation of qi and blood, and internal accumulation of toxic evil. The three energizers preside over all qi and dredge the waterways. When the three energizers pass the vital energy and the qi is stagnant and not smooth, it gathers and turns into dampness, and stagnation turns into fire, affecting the blood circulation, and it is easy to have the situation of qi stagnation and blood stasis. Qi stagnation leads to blood stasis, and blood stasis becomes accumulation, and stasis turns into heat, which may eventually develop into gastric cancer. The middle energizer includes the spleen, stomach, liver and gallbladder, which is the source of food digestion and absorption and qi and blood biochemistry. As a malignant tumor of the digestive system, gastric cancer is closely related to the functional abnormality of the middle energizer. When the middle energizer is blocked, the spleen and stomach function is impaired, the qi and blood biochemistry is passive, and the qi mechanism rises and falls abnormally, it may lead to the accumulation of pathological products such as phlegm dampness and blood stasis, and then form gastric cancer. Gastric cancer patients often have toxic evils such as damp heat, phlegm turbidity, and blood stasis in their bodies. These toxic evils accumulate in the three energizers, leading to dysfunction of the internal organs, deficiency of righteous qi, and eventually induce gastric cancer. Therefore, when treating gastric cancer, we should focus on comprehensive treatment such as unblocking the three burners, harmonizing qi and blood, detoxifying and dispersing nodules, as well as overall conditioning. Therefore, this prescription uses Lithospermum officinale and Semen Armeniacae Sinensis as the main ingredients. Lithospermum officinale promotes blood circulation and removes blood stasis, clears away heat and removes dampness, disperses nodules and reduces swelling, while Semen Armeniacae Sinensis soothes the liver and regulates qi, detoxifies and relieves pain. The two regulate qi stagnation, clear away blood stasis and heat, and prevent the Shaoyang phase fire from moving rashly, and together dredge the qi and water channels of the triple burner, so that qi and blood can flow without stagnation and water metabolism is normal; Polygonum cuspidatum, Coptis chinensis and Linderae scutellariae are the auxiliary ingredients. Polygonum cuspidatum helps Lithospermum officinale to enhance the effect of promoting blood circulation and removing blood stasis, and can clear away heat and remove toxins, disperse blood stasis and relieve pain, enter the liver and gallbladder meridians, and regulate the triple burner; Coptis chinensis helps Lithospermum officinale to enhance the effect of clearing blood stasis and heat, and can also take into account drying dampness and purging fire and detoxification; Linderae scutellariae helps Linderae scutellariae to enhance the effect of promoting qi and relieving pain and detoxification, and can also take into account soothing the liver and regulating qi, dispersing nodules and promoting diuresis; Angelica dahurica and ginseng can penetrate the evil and open up the way out for the pathogenic factors, so that the pathogenic factors can be discharged smoothly, which can replenish the vital energy, nourish the spleen and benefit the lungs, and enhance the body's positive energy and disease resistance. The combination of the two can not only expel evil spirits but also strengthen the body, achieving better therapeutic effects. The two medicines are used together as adjuvants, and the monarch and ministers work together to regulate qi and harmonize the stomach, disperse knots and relieve pain, remove blood stasis and detoxify, and dredge the three burners.

[0028] Beneficial Effects

[0029] The Chinese medicine prescription of the present invention is developed based on the pathogenesis of gastric cancer, and is developed on the basis of theoretical research on the triple energizer system and laboratory research. It has the functions of regulating qi and harmonizing the stomach, dispersing knots and relieving pain, removing blood stasis and detoxifying, and dredging the triple energizer, and has a good effect on the treatment of gastric cancer. The overall treatment principle is to strengthen the body and eliminate evil, and the treatment method is to regulate qi and harmonize the stomach, dispersing knots and relieving pain, and removing blood stasis and detoxifying. Experiments have shown that the Chinese medicine prescription of the present invention has a good anti-gastric cancer effect and low toxicity, and is a major innovation in the treatment of gastric cancer. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 The effect of Chinese medicine formula on the epigenetic state of tumor cells.

[0031] Figure 2 It is the growth inhibition rate of tumor cells by the Chinese medicine formula.

[0032] Figure 3 The effect of Chinese medicine formula on the migration ability of tumor cells. In the figure, ns means no statistical significance. DETAILED DESCRIPTION

[0033] The specific implementation modes of the present invention are further described in detail below in conjunction with the embodiments.

[0034] Example 1

[0035] A traditional Chinese medicine formula for treating gastric cancer comprises the following raw materials in parts by weight: 3 parts of angelica dahurica, 3 parts of coptis root, 3 parts of ginseng, 15 parts of lithospermum officinale, 10 parts of cyperus rotundus, 9 parts of polygonum cuspidatum and 3 parts of lindera striata.

[0036] Its preparation method is:

[0037] (1) Wash and chop Angelica dahurica, Coptis chinensis, Panax ginseng, Lithospermum officinale, Fructus cyrtonemae, Polygonum cuspidatum, and Linderae sibiricum respectively, and grind them into coarse powder;

[0038] (2) adding 8 times the volume of each of the above raw materials and soaking in 70% (v / v) ethanol solution; covering with plastic wrap and tin foil to avoid light at room temperature, stirring and shaking twice with a glass rod from time to time, and soaking for a total of 24 hours; then adding the soaked raw materials into a reflux bottle for extraction, the temperature is 90°C, boiling for 1 hour, filtering, taking the filtrate, recovering the residue, repeating the above steps again, refluxing extraction, boiling, filtering, taking the filtrate, filtering with three layers of filter paper, and connecting an external vacuum pump to accelerate the filtration speed;

[0039] (3) Mix the two filtrates of each API, place them on a 45°C rotary evaporator to remove ethanol from the filtrate, pour them into a crucible, evaporate the water in a water bath at 78°C, freeze them in a -80°C refrigerator, and finally dry them into a solid state using a freezer and crush them into powder;

[0040] (4) Mix all the raw material drug powders and store at -20°C.

[0041] Example 2

[0042] A traditional Chinese medicine formula for treating gastric cancer comprises the following raw materials in parts by weight: 6 parts of angelica dahurica, 6 parts of coptis root, 3 parts of ginseng, 30 parts of lithospermum officinale, 15 parts of cyperus rotundus, 12 parts of polygonum cuspidatum and 9 parts of lindera striata.

[0043] Its preparation method is:

[0044] (1) Wash and chop Angelica dahurica, Coptis chinensis, Panax ginseng, Lithospermum officinale, Fructus cyrtonemae, Polygonum cuspidatum, and Linderae sibiricum respectively, and grind them into coarse powder;

[0045] (2) adding 8 times the volume of each of the above raw materials and soaking in 75% (v / v) ethanol solution; covering with plastic wrap and tin foil to avoid light at room temperature, stirring and shaking twice with a glass rod from time to time, and soaking for a total of 24 hours; then adding the soaked raw materials into a reflux bottle for extraction, the temperature is 90°C, boiling for 1 hour, filtering, taking the filtrate, recovering the residue, repeating the above steps again, refluxing extraction, boiling, filtering, taking the filtrate, filtering with three layers of filter paper, and connecting an external vacuum pump to accelerate the filtration speed;

[0046] (3) Mix the two filtrates of each API, place them on a 42°C rotary evaporator to remove ethanol from the filtrate, pour them into a crucible, evaporate the water in a water bath at 80°C, freeze them in a -80°C refrigerator, and finally dry them into a solid state using a freezer and crush them into powder;

[0047] (4) Mix all the raw material drug powders and store at -20°C.

[0048] Example 3

[0049] A traditional Chinese medicine formula for treating gastric cancer comprises the following raw materials in parts by weight: 7 parts of angelica dahurica, 7 parts of coptis root, 7 parts of ginseng, 20 parts of lithospermum officinale, 12 parts of cyperus rotundus, 10 parts of polygonum cuspidatum and 6 parts of lindera stolonifera.

[0050] Its preparation method is:

[0051] (1) Wash and chop Angelica dahurica, Coptis chinensis, Panax ginseng, Lithospermum officinale, Fructus cyrtonemae, Polygonum cuspidatum, and Linderae sibiricum respectively, and grind them into coarse powder;

[0052] (2) adding 9 times the volume of each of the above raw materials and soaking in 70% (v / v) ethanol solution; covering with plastic wrap and tin foil to avoid light at room temperature, stirring and shaking twice with a glass rod from time to time, and soaking for a total of 36 hours; then adding the soaked raw materials into a reflux bottle for extraction, boiling at 95°C for 1 hour, filtering, taking the filtrate, recovering the residue, repeating the above steps of reflux extraction again, boiling, filtering, taking the filtrate, filtering with three layers of filter paper, and connecting an external vacuum pump to accelerate the filtration speed;

[0053] (3) Mix the two filtrates of each API, place them on a 50°C rotary evaporator to remove ethanol from the filtrate, pour them into a crucible, evaporate the water in a water bath at 70°C, freeze them in a -80°C refrigerator, and finally dry them into a solid state using a freezer and crush them into powder;

[0054] (4) Mix all the raw material drug powders and store at -20°C.

[0055] Example 4

[0056] A traditional Chinese medicine formula for treating gastric cancer comprises the following raw materials in parts by weight: 9 parts of angelica dahurica, 9 parts of coptis root, 9 parts of ginseng, 30 parts of lithospermum officinale, 15 parts of cyperus rotundus, 15 parts of polygonum cuspidatum and 9 parts of lindera striata.

[0057] Its preparation method is:

[0058] (1) Wash and chop Angelica dahurica, Coptis chinensis, Panax ginseng, Lithospermum officinale, Fructus cyrtonemae, Polygonum cuspidatum, and Linderae sibiricum respectively, and grind them into coarse powder;

[0059] (2) Add 10 times the volume of each of the above raw materials and soak in 75% (v / v) ethanol solution; cover with plastic wrap and tin foil to avoid light at room temperature, stir and shake twice with a glass rod from time to time, and soak for a total of 48 hours; then add the soaked raw materials to a reflux bottle for extraction, boil at 97°C for 1 hour, filter, take the filtrate, recover the residue, repeat the above steps again, reflux extraction, boil, filter, take the filtrate, filter with three layers of filter paper, and connect an external vacuum pump to speed up the filtration speed;

[0060] (3) Mix the two filtrates of each API, place them on a 45°C rotary evaporator to remove ethanol from the filtrate, pour them into a crucible, evaporate the water in a water bath at 75°C, freeze them in a -80°C refrigerator, and finally dry them into a solid state using a freezer and crush them into powder;

[0061] (4) Mix all the raw material drug powders and store at -20°C.

[0062] Experiment on the inhibition of tumor growth by anti-gastric cancer Chinese medicine formula:

[0063] 1 Materials

[0064] 1.1 Cell lines

[0065] Human gastric cancer cell line AGS; human gastric cancer cell line HGC-27; normal human gastric mucosal epithelial cell line GES-1 (provided by the Key Laboratory of Traditional Chinese Medicine Prescription and Syndrome Signal Transduction of Henan Province).

[0066] 1.2 Reagents

[0067] Thiazole blue (MTT) was provided by Beijing Solebow Technology Co., Ltd., catalog number M8180.

[0068] 2 Methods

[0069] 2.1 Cell culture

[0070] AGS cells, HGC-27 cells and GES-1 cells were cultured in 1640 medium containing 10% (v / v) fetal bovine serum (FBS), 5958 mg / L 4-hydroxyethylpiperazineethanesulfonic acid (hepes), 100 u / mL penicillin and 100 μg / mL streptomycin, respectively, and placed in a humidity incubator at 37°C and 5% CO2. The cells were passaged every 1 to 2 days, and logarithmically growing cells were used in all experiments.

[0071] 2.2 Observation of gastric cancer cell growth status in normal group and Chinese medicine group

[0072] The three cell lines in 2.1 were cultured to the logarithmic growth phase. When they covered more than 70% of the 100 mm culture dish area, they were digested with trypsin containing ethylenediaminetetraacetic acid disodium salt (EDTA) and then incubated at 1.2 × 10 5 The cells were inoculated at a density of 100 / mL in a 6-well plate, and a blank group and a drug group (the Chinese medicine formula in Example 1) were set up. The drugs were added every other day. The blank group was directly added with 10% (v / v) fetal bovine serum in 1640 medium, 2 mL / well. The drug group was added with 10% (v / v) fetal bovine serum in 1640 medium with the same concentration of Chinese medicine as the Chinese medicine extract in 2.3 (the Chinese medicine formula in Example 1), 2 mL / well, and continued to be cultured for 48 hours. The cells were taken out for observation and recorded with a microscope.

[0073] 2.3 MTT assay to determine the dose-effect relationship of cell growth inhibition

[0074] AGS cells, HGC-27 cells and GES-1 cells were cultured at 5×10 3 Each well / 200 μL was inoculated in a 96-well flat culture plate and cultured in a 37°C, 5% CO2 incubator for 24 hours. After 24 hours, the cells completely adhered to the wall and grew, the culture medium in the well was discarded, and a control group and 5 Chinese medicine groups were set up, with 3 duplicate wells in each group. The control group was added with 200 μL of 1640 culture medium containing 10% (v / v) fetal bovine serum, and the 5 Chinese medicine groups were added with 200 μL of Chinese medicine extracts with concentrations of 25, 50, 100, 200, and 400 μg / mL (the Chinese medicine formula in Example 1, the solvent is 1640 culture medium containing 10% (v / v) fetal bovine serum), and it was noted that it was added slowly against the well wall. Continue to culture for 48 hours, remove the culture supernatant, add 100 mL of 0.5 mg / mL MTT solution to each well, continue to culture for 4 hours, carefully remove the supernatant, and add 150 μL of dimethyl sulfoxide (DMSO) to dissolve the purple precipitate, and measure the OD value at a wavelength of 490 nm using an enzyme marker. According to the formula:

[0075] Inhibition rate (%) = (1-average A 490值 / Average A of control wells 490值 )×100%

[0076] IC30 Value and IC 50 Calculation of values: After the above experiment was repeated three times or more, the obtained experimental data (cell inhibition rate) were imported into SPSS for analysis and calculation.

[0077] 2.4 Cell scratch assay to detect cell migration ability

[0078] AGS cells were cultured at 6 × 10 5 100 μL / mL was inoculated into a 6-well plate with horizontal lines drawn on the bottom of the dish in advance, and 3 duplicate wells were set up in each well. The plate was cultured in a 37°C, 5% CO2 incubator for 24 hours until the cell monolayer was fully covered. A straight line from top to bottom was drawn in the middle of the well along the ruler using a sterile 200 μL pipette tip, and the supernatant and floating cells were removed. The cells were washed 3 times with PBS buffer. The control group was added with 1640 medium containing 2% (v / v) fetal bovine serum, and the drug group was treated with the IC screened in 2.3. 30 (Drug concentration when the inhibition rate reaches 30%), add 1640 culture medium containing 2% (v / v) fetal bovine serum containing 20μg / mL, 40μg / mL, and 80μg / mL of Chinese medicine extract (Chinese medicine formula in Example 1), and culture in an incubator at 37°C and 5% CO2. Take photos and record at 0h, 24h, and 48h. Draw out the liquid before each photo, wash gently with PBS buffer 1-2 times, and replace the liquid to remove floating cells to avoid affecting the objectivity and aesthetics of the photo. The experiment was repeated three times. The migration area was calculated using ImageJ software. The scratch healing rate was calculated according to the formula:

[0079] Scratch healing rate (%) = (0h-24h / 48h) / 0h×100%

[0080] 2.5 Statistical methods

[0081] The experimental data were tested for homogeneity of variance and normality using the software spss 25.0. GraphPad Prism 8 software was used for graphical analysis.

[0082] 3 Experimental results

[0083] 3.1 Effects of Chinese herbal medicine on the growth and morphology of human gastric cancer cell lines AGS, HGC-27, and GES-1

[0084] Under the microscope, the cells in the blank group grew attached to the wall, with clear cell boundaries, obvious nuclear division phase, and pseudopodia extending; the cell outlines in the drug group were very clear, the cytoplasm was extremely rough, and it was filled with a large amount of granular substances. With the increase of drug concentration, large-scale necrosis blank field and vacuolar appearance appeared (see Figure 1 ). This shows that the state of tumor cells deteriorated after taking the drug, and even died in pieces.

[0085] 3.2 Effect of Chinese medicine formula on tumor cell growth (inhibition rate)

[0086] The Chinese medicine formula can inhibit the growth of human gastric cancer cell lines AGS and HGC-27, and the cell growth inhibition shows a dose-effect dependence with the increase of drug concentration (see Table 1 and Figure 2 ). Excessive doses of Chinese medicine have a strong inhibitory effect on all cells, but at a certain concentration, the inhibition rate on the normal human gastric mucosal epithelial cell line GES-1 is low. It can be seen that the Chinese medicine formula has a strong killing effect on cancer cells, a small killing effect on normal cells, and a small side effect. At the same time, it can be calculated that the IC of AGS at 24h 30 The value is 52μg / mL, IC 50 The value is 142 μg / mL, and the IC 30 The value is 102μg / mL, IC 50 The value is 195 μg / mL, and the IC 30 The value is 203 μg / mL, IC 50 The value was 269 μg / mL; the IC of AGS at 48 h 30 The value is 38μg / mL, IC 50 The value is 120 μg / mL, and the IC 30 The value is 57 μg / mL, IC 50 The value is 150 μg / mL, and the IC 30 The value is 157 μg / mL, IC 50 The value is 158μg / mL.

[0087] Table 1: Changes of Chinese medicine inhibition rate on tumor cell growth with drug concentration (unit: μg / mL)

[0088]

[0089] Note: Compared with 0 μg / mL in each group, *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001.

[0090] 3.3 Scratch test to detect the effect of Chinese medicine formula on the proliferation of AGS gastric cancer cells

[0091] After the scratch was treated with drugs, photos were taken at 0h, 24h, and 48h. The results of the cell scratch experiment showed that the wound healing rate of AGS cell migration decreased with the increase of drug concentration (see Figure 3 , see Table 2).

[0092] Table 2: Comparison of different concentrations of Chinese medicine formula on the healing rate of AGS cell scratches ( n=3)

[0093]

[0094] Note: Compared with 0 μg / mL in each group, **P<0.01, ***P<0.001, ****P<0.0001.

[0095] 4 Conclusion

[0096] 4.1 The experimental results of anti-gastric cancer Chinese medicine formula showed a good anti-gastric cancer effect, which was dose-dependent.

[0097] 4.2 The anti-gastric cancer Chinese medicine formula can effectively inhibit the growth of gastric cancer cells AGS and HGC-27 in a dose-dependent manner, and has little lethality to normal gastric mucosal epithelial cells GES-1. This drug formula has a good effect on inhibiting tumor growth and has few side effects.

[0098] 4.3 By observing and quantifying the ability of gastric cancer cells to migrate to the blank area, it was found that the migration ability of gastric cancer cells intervened by the Chinese medicine formula decreased significantly in a dose-dependent manner. The anti-gastric cancer Chinese medicine formula has a good anti-gastric cancer effect and low toxicity. It is an innovation in the treatment of gastric cancer and has significant clinical significance.

Claims

1. A Chinese medicine formula for treating gastric cancer, characterized in that: The invention comprises the following raw materials in parts by weight: 3-9 parts of angelica dahurica, 3-9 parts of coptis root, 3-9 parts of ginseng, 15-30 parts of lithospermum officinale, 10-15 parts of cyperus rotundus, 9-15 parts of polygonum cuspidatum and 3-9 parts of lindera striata.

2. The Chinese medicine formula for treating gastric cancer according to claim 1, characterized in that: The invention comprises the following raw materials in parts by weight: 3 parts of angelica dahurica, 3 parts of coptis root, 3 parts of ginseng, 15 parts of lithospermum officinale, 10 parts of cyperus rotundus, 9 parts of polygonum cuspidatum and 3 parts of lindera stolonifera.

3. A method for preparing a Chinese medicine formula for treating gastric cancer as claimed in claim 1 or 2, characterized in that: The raw materials are extracted with ethanol respectively, then dried, and the dried extracts are mixed to obtain the product.

4. The preparation method according to claim 3, characterized in that: The specific steps include: (1) Wash and chop Angelica dahurica, Coptis chinensis, Panax ginseng, Lithospermum officinale, Fructus cyrtonemae, Polygonum cuspidatum, and Linderae sibiricum respectively, and grind them into coarse powder; (2) adding ethanol solution to each raw material drug respectively, soaking at room temperature in the dark, then adding it to a reflux bottle for extraction, boiling, filtering, taking the filtrate, recovering the drug residue, repeating the above extraction steps, and taking the filtrate; (3) The two filtrates of each raw material drug are mixed, respectively, placed on a rotary evaporator to remove ethanol, then evaporated in a water bath, placed in a refrigerator for freezing, and finally freeze-dried and crushed into powder; (4) Mix the powders of the raw materials to obtain the product.

5. The preparation method according to claim 4, characterized in that: The volume concentration of the ethanol solution in step (2) is 70-75%, and the amount used is 8-10 times the volume of the raw material drug.

6. The preparation method according to claim 4, characterized in that: The soaking time in step (2) is 24-48 hours.

7. The preparation method according to claim 4, characterized in that: The reflux temperature in step (2) is 90-100°C.

8. The preparation method according to claim 4, characterized in that: The evaporation temperature of the rotary evaporator in step (3) is 42-50°C.

9. The preparation method according to claim 3, characterized in that: In step (3), the water bath temperature is 70-80°C.

10. Use of the Chinese medicine formula according to claim 1 or 2 in preparing a drug for treating gastric cancer.

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