Integrated microfluid driving and real-time observation device

Abstract

The invention provides an integrated microfluid driving and real-time observation device. The integrated microfluid driving and real-time observation device comprises a micro-fluidic chip loading device, an imaging assembly, a liquid driving assembly, a display interaction assembly and a control assembly which are integrally mounted in a shell, the shell comprises a complete machine bottom plate, a back plate, a side wall plate, a first cabin door and a second cabin door, the first cabin door and the second cabin door are rotationally connected with the back plate, and the shell is divided into a first cabin body and a second cabin body; the micro-fluidic chip loading device is arranged in the first cabin, and the imaging assembly, the liquid driving assembly and the control assembly are arranged in the second cabin; the display interaction assembly comprises a control touch screen and a display touch screen, the control touch screen is arranged in the first cabin body, and the display touch screen is arranged on the outer wall of the second cabin door. The driving device is compact in structure, complete in function and high in integration level, different types of micro-fluidic chips can be driven according to actual requirements, and applications such as micro-droplet generation, single-cell wrapping and double-wrapping microsphere preparation are achieved.

Description

Technical field:

[0001] The invention belongs to the technical field of microfluid control devices, and in particular relates to an integrated microfluid driving and real-time observation device. Background technology:

[0002] Microfluidics refers to a technology that uses microchannels to manipulate and process tiny fluids. It is an emerging technology that integrates multiple disciplines such as physics, chemistry, materials, and biology. The emergence of microfluidics can integrate operations such as sample preparation, separation, reaction, and detection in biological, chemical, and medical analysis onto a chip, greatly simplifying the operating process, so it is also called a chip laboratory. Generally, microfluidic chips are designed with multi-channel fluid control. For applications related to material preparation, a product collection device needs to be set up, and some also require a real-time observation device to observe the real-time working status of the flow inside the chip. At present, most microfluidic drive devices have only a single function, or use a single functional module to form a distributed working platform. This often leads to a loose structure of the entire platform, a large volume, and it is impossible to achieve unified control, which brings many inconveniences to the user. Based on this, the present invention provides an integrated microfluidic drive and real-time observation device to solve the above problems. Summary of the invention:

[0003] The purpose of the present invention is to provide an integrated microfluidic drive and real-time observation device to address the deficiencies of the prior art, thereby solving the problems of the existing microfluidic control platform, such as single function, loose structure, large size and inability to achieve unified control, simplifying the operation difficulty and improving work efficiency.

[0004] In order to achieve the above object, the present invention adopts the following technical solutions:

[0005] The present invention provides an integrated microfluidic drive and real-time observation device, comprising: a microfluidic chip loading device, an imaging component, a liquid drive component and a display interaction component; the microfluidic chip loading device, the imaging component, the liquid drive component and the display interaction component are integrated and installed inside a shell.

[0006] Furthermore, the shell includes a shell body and a door; the shell body includes a whole machine bottom plate, a back plate and side wall plates; the door includes a first door and a second door, the first door and the second door are respectively rotatably connected to the back plate, and divide the shell into a first cabin body and a second cabin body; the microfluidic chip loading device is arranged inside the first cabin body, and the imaging component and the liquid drive component are arranged inside the second cabin body; the display interaction component includes a control touch screen and a display touch screen, the control touch screen is arranged inside the first cabin body, and is used for users to control the light source, liquid drive component and imaging system in the device, and the display touch screen is arranged on the outer wall of the second door, and is used to display the real-time working picture of the microfluidic chip taken by the camera.

[0007] Furthermore, the display touch screen may be replaced by an external computer display screen, or the display touch screen and the control touch screen functions may be integrated into one touch screen.

[0008] Furthermore, a first cabin is provided inside the first cabin body, a second cabin is provided inside the second cabin body, and a partition plate is provided between the first cabin and the second cabin; the microfluidic chip loading device is provided inside the first cabin, and the control touch screen is installed on the bottom plate of the whole machine; a pallet is provided in the second cabin, dividing the second cabin into two layers, the imaging component is provided on the upper layer and fixed on the pallet, and a power supply and a control device are provided on the lower layer of the second cabin; the liquid drive component includes one or more injection pumps, and the injection pumps are fixedly installed on the outer surface of the second cabin and / or the bottom plate of the whole machine.

[0009] Furthermore, the injection pump can be replaced by a pneumatic pump, a plunger pump or a peristaltic pump.

[0010] Furthermore, the liquid drive assembly includes a first injection pump, a second injection pump and a third injection pump; a middle layer plate is provided on the side wall of the outer surface of the second cabin; the first injection pump is fixedly set on the top layer plate of the second cabin, the second injection pump is fixedly set on the middle layer plate, and the third injection pump is fixedly set on the bottom plate of the whole machine.

[0011] Furthermore, the control device includes an injection pump control circuit board, a main control circuit board and a power supply filter.

[0012] Furthermore, the microfluidic chip in the microfluidic chip loading device corresponds to the position of the lens of the imaging component, and a camera extension port is provided on the partition plate, and the size of the camera extension port is larger than the size of the lens.

[0013] Furthermore, the second cabin has a rectangular structure, including a front lower support frame, a front upper support frame, a rear upper support frame and a rear lower support frame; the rear upper support frame and the rear lower support frame are fixed on the back plate, the front lower support frame is vertically fixed on the bottom plate of the whole machine, and the front upper support frame is fixed on the front lower support frame; panels are arranged outside the front upper support frame and the front lower support frame.

[0014] Furthermore, the microfluidic chip loading device includes a supporting base, a multifunctional bracket, a chip mounting plate, an ultraviolet curing lamp and an illumination light source; the multifunctional bracket is installed on the supporting base; the chip mounting plate, the ultraviolet curing lamp and the illumination light source are respectively installed on the multifunctional bracket.

[0015] Furthermore, the lighting source is a white LED, and other suitable lighting sources may also be used.

[0016] Furthermore, the multifunctional bracket includes a vertical rod, a first horizontal bracket and a second horizontal bracket; the vertical rod is fixed on a supporting base; the first horizontal bracket is adjustably mounted on the vertical rod, and the chip mounting plate is fixedly mounted on the first horizontal bracket; the second horizontal bracket is fixedly mounted on the bottom of the vertical rod, a UV lamp bracket is fixedly provided on the second horizontal bracket, and the UV curing lamp is mounted on the UV lamp bracket; the lighting light source is fixedly mounted on the vertical rod, and the lighting light source is distributed behind the chip mounting plate.

[0017] Furthermore, the first horizontal bracket includes a horizontal connecting member and a U-shaped slider with a single-sided opening; the U-shaped slider is slidably connected to the vertical rod, the horizontal connecting member is fixed on one side of the slider, and the chip mounting plate is fixedly installed on the horizontal connecting member; a threaded hole is provided on the U-shaped slider, and the first horizontal bracket is fixed by screwing the bolt fixing member into the threaded hole, passing through the threaded hole and tightening the side wall of the vertical rod.

[0018] Furthermore, the UV lamp bracket includes an L-shaped connecting rod and a spherical rotating frame; the L-shaped connecting rod is fixedly connected to the vertical rod, and the spherical rotating frame is fixedly connected to the L-shaped connecting rod; a U-shaped slot is provided on the top of the spherical rotating frame, and the UV curing lamp is fixed in the U-shaped slot.

[0019] Furthermore, the support base includes an XY-axis displacement platform, a Z-axis displacement platform and a connecting plate; the connecting plate is installed on the top of the XY-axis displacement platform, and the Z-axis displacement platform is installed on the connecting plate.

[0020] Furthermore, an operating hole is opened on the side wall of the shell, and the adjustment knobs of the XY-axis displacement platform and the Z-axis displacement platform pass through the operating hole and extend to the outside of the shell.

[0021] Furthermore, the chip mounting plate includes a fixing plate, two groups of chip fixing slides and two groups of fixing bolts; the fixing plate includes a central part and edge parts on both sides of the central part; the central part is provided with a first groove adapted to the size of the microfluidic chip, and an opening for light transmission is provided in the middle position of the first groove; the edge part is provided with one or more horizontally through-going second grooves, the chip fixing slide is provided with a bump adapted to the size of the second groove, and the chip fixing slide is slidably connected to the edge part of the fixing plate through the second groove and the bump; the edge part is provided with a threaded hole, and the chip fixing slide is provided with a through hole corresponding to the position of the threaded hole. When the chip fixing slide slides to the fixed position, the chip fixing slide is fixed by the fixing bolt passing through the through hole and being threadedly connected to the fixing plate.

[0022] Furthermore, the imaging assembly includes a camera, a camera lens and an adjustable supporting device; the adjustable supporting device includes a motor, a camera fixing frame, a lens supporting frame, a screw module, a slide rail, a slider and a slide rail base; the screw module includes a screw base, a screw and a slide seat, the screw base includes a base plate, one end of the base plate is provided with a vertical side plate, and the other end is equipped with a motor; one end of the screw is rotatably connected to the side plate, and the other end is connected to the output end of the motor; the slide seat is threadedly connected to the screw, and the bottom of the slide seat is slidably connected to the screw base; the camera fixing frame is fixedly connected to the slide seat, and the camera is installed on the camera fixing frame; the slide rail is fixedly installed on the slide rail base, the slider is fixedly installed on the bottom of the lens supporting frame, the slider is slidably connected to the slide rail, and the camera lens is installed on the lens supporting frame.

[0023] Furthermore, a foot pad is provided at the bottom of the shell.

[0024] Beneficial effects of the present invention:

[0025] Compared with the prior art, the present invention has the following advantages: the integrated microfluidic drive and real-time observation device described in the present invention integrates an integrated microfluidic chip loading platform, a fully automatic imaging component, a liquid drive component and a display interaction component in an integrated device, which can realize multi-channel liquid drive, real-time observation of chip working status, ultraviolet light curing and other functions, and is convenient for users to prepare various micro droplets. The device is fully functional and highly integrated, and users can use it right away without much preliminary preparation. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 This is a schematic diagram of the structure of the device of the present invention in an open cover state;

[0027] Figure 2 This is a schematic diagram of the structure of the device of the present invention in a closed state;

[0028] Figure 3 This is a schematic diagram of the structure of the device of the present invention when the cover is removed;

[0029] Figure 4 This is a schematic diagram of the structure of the first cabin and the second cabin inside the device of the present invention;

[0030] Figure 5 This is a schematic diagram of the structure of the microfluidic chip loading device of the present invention;

[0031] Figure 6 This is a schematic diagram of the structure of the liquid drive assembly of the present invention;

[0032] Figure 7 This is a schematic diagram of the structure of the fully automatic imaging component of the present invention;

[0033] Figure 8It is a schematic diagram of the structure of the multifunctional support in the microfluidic chip loading device of the present invention;

[0034] Fig. 9 This is a schematic diagram of the structure of the microfluidic chip mounting plate of the present invention.

[0035] The symbols in the accompanying drawings are:

[0036] 1. Microfluidic chip loading device; 101. XY axis displacement platform; 102. Z axis displacement platform; 103. Connecting plate; 104. UV lamp bracket; 105. UV curing lamp; 106. Vertical rod; 107. First horizontal bracket; 108. Fixing plate; 109. Chip fixing slide; 110. Double emulsion microfluidic chip; 111. Illumination light source; 112. Second horizontal bracket; 2. Liquid drive assembly; 21. First injection pump; 22. Second injection pump; 23. Third injection pump; 3. Imaging assembly; 301. Camera; 302. Camera lens; 303. Camera fixing frame; 304. Lens support frame; 305. Screw module ; 306, slide rail; 307, slider; 308, slide rail base; 401, whole machine bottom plate; 402, middle layer plate; 404, front lower support frame; 405, rear lower support frame; 406, front upper support frame; 407, rear upper support frame; 408, foot pad; 5, shell; 51, first hatch; 52, second hatch; 53, side wall plate; 531, camera extension port; 532, operation hole; 61, display touch screen; 62, control touch screen; 701, power supply; 702, injection pump control circuit board; 703, main control circuit board; 704, power supply filter; 705, USB socket; 8, second cabin; 9, first cabin. Specific implementation method:

[0037] In order to make the purpose, technical solution and advantages of the present invention clearer, the technical solution in the embodiment of the present invention will be clearly and completely described below in conjunction with the relevant drawings. Obviously, the described embodiment is a part of the embodiment of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention. The implementation conditions not specified in the embodiments are usually the conditions in routine experiments.

[0038] Embodiment 1:

[0039] An embodiment of the present invention provides an integrated microfluidic driving and real-time observation device, comprising: a microfluidic chip loading device, an imaging component, a liquid driving component and a display interaction component; the microfluidic chip loading device, imaging component, liquid driving component and display interaction component are integrated and installed inside the shell 5.

[0040] Reference Figures 1 to 3In this embodiment, the shell 5 includes a shell body and a hatch, and a foot pad 408 is arranged at the bottom of the shell 5; the shell body includes a whole machine bottom plate 401, a back plate and a side wall plate 53; the hatch includes a first hatch 51 and a second hatch 52, the first hatch 51 and the second hatch 52 are respectively rotatably connected to the back plate, can be opened and closed independently, and divide the shell 5 into a first cabin and a second cabin; the microfluidic chip loading device is arranged inside the first cabin, and the imaging component and the liquid drive component are arranged inside the second cabin; the display interaction component includes a control touch screen 62 and a display touch screen 61, the control touch screen 62 is arranged inside the first cabin, and is used for the user to control the light source, liquid drive component and imaging system in the device, and the display touch screen 61 is arranged on the outer wall of the second cabin 52, and is used to display the real-time working picture of the microfluidic chip taken by the camera.

[0041] Embodiment 2:

[0042] The embodiment of the present invention provides an integrated microfluidic driving and real-time observation device, whose main structure is the same as that of embodiment 1, except that: this embodiment limits the internal settings of the first cabin and the second cabin.

[0043] Specifically, refer to Figures 1 to 4 In this embodiment, a first cabin 9 is provided inside the first cabin body, a second cabin 8 is provided inside the second cabin body, and a partition plate is provided between the first cabin 9 and the second cabin 8; the microfluidic chip loading device is provided inside the first cabin 9, and the control touch screen 62 is installed on the bottom plate 401 of the whole machine; a pallet is provided in the second cabin 8, dividing the second cabin 8 into two layers, the imaging component is provided on the upper layer and fixed on the pallet, and a power supply 701 and a control device are provided on the lower layer of the second cabin 8, and the control device includes an injection pump control circuit board 702, a main control circuit board 703 and a power filter 704.

[0044] In this embodiment, the second cabin 8 is of a rectangular structure, including a front lower support frame 404, a front upper support frame 406, a rear upper support frame 407 and a rear lower support frame 405; the rear upper support frame 407 and the rear lower support frame 405 are fixed on the back plate, the front lower support frame 404 is vertically fixed on the bottom plate 401 of the whole machine, and the front upper support frame 406 is fixed on the front lower support frame 404; the front upper support frame 406 and the front lower support frame 404 are provided with panels on the outside.

[0045] In this embodiment, the microfluidic chip in the microfluidic chip loading device corresponds to the lens position of the imaging component, and a camera extension port 531 is provided on the partition plate. The size of the camera extension port 531 is larger than the size of the lens, so that the camera lens can be extended through the camera extension port 531 and approach the microfluidic chip. The liquid drive component includes one or more injection pumps, which are fixedly mounted on the outer surface of the second chamber 8 and / or the bottom plate 401 of the whole machine.

[0046] Embodiment 3:

[0047] The embodiment of the present invention provides an integrated microfluidic driving and real-time observation device, whose main structure is the same as that of Example 2, except that: this embodiment limits the arrangement of the liquid driving component.

[0048] Specifically, refer to Figure 3 and 6 In this embodiment, the liquid drive assembly includes a first injection pump 21, a second injection pump 22 and a third injection pump 23. A middle plate 402 is provided on the panel of the second cabin 8. The first injection pump 21 is fixedly arranged on the top plate of the second cabin 8, the second injection pump 22 is fixedly arranged on the middle plate 402, and the third injection pump 23 is fixedly arranged on the bottom plate 401 of the whole machine, in a stepped arrangement.

[0049] Embodiment 4:

[0050] The embodiment of the present invention provides an integrated microfluidic driving and real-time observation device, whose main structure is the same as that of Example 3, except that: this embodiment limits the structure of the microfluidic chip loading device.

[0051] Specifically, refer to Figure 5 and 8 In this embodiment, the microfluidic chip loading device includes a support base, a multifunctional bracket, a chip mounting plate, an ultraviolet curing lamp 105 and an illumination light source 111; the multifunctional bracket is mounted on the support base; the chip mounting plate, the ultraviolet curing lamp 105 and the illumination light source 111 are respectively mounted on the multifunctional bracket. The support base can be set as a fixed structure or a three-dimensional adjustment structure.

[0052] In this embodiment, the multifunctional bracket includes a vertical rod 106, a first horizontal bracket 107 and a second horizontal bracket 112; the vertical rod 106 is fixed on a supporting base; the first horizontal bracket 107 is adjustably mounted on the vertical rod 106, and the chip mounting plate is fixedly mounted on the first horizontal bracket 107; the second horizontal bracket 112 is fixedly mounted on the bottom of the vertical rod 106, and a UV lamp bracket 104 is fixedly arranged on the second horizontal bracket 112, and the UV curing lamp 105 is mounted on the UV lamp bracket 104; the lighting light source 111 is fixedly mounted on the vertical rod 106, and the lighting light source 111 is distributed behind the chip mounting plate, and the lighting light source 111 is substantially consistent with the center position of the chip mounting plate.

[0053] In this embodiment, the first horizontal bracket 107 includes a horizontal connecting piece and a U-shaped slider with a single-sided opening; the U-shaped slider is slidably connected to the vertical rod 106, the horizontal connecting piece is fixed on one side of the slider, and the chip mounting plate is fixedly installed on the horizontal connecting piece; a threaded hole is provided on the U-shaped slider, and the first horizontal bracket 107 is fixed by screwing a knurled screw into the threaded hole, passing through the threaded hole and tightening the side wall of the vertical rod 106. After loosening the knurled screw, the first horizontal bracket 107 can slide up and down along the vertical rod 106, and the first horizontal bracket 107 can be fixed after tightening the knurled screw.

[0054] In this embodiment, the UV lamp bracket 104 includes an L-shaped connecting rod and a spherical rotating frame, the L-shaped connecting rod is fixedly connected to the vertical rod 106, and the spherical rotating frame is fixedly connected to the L-shaped connecting rod; a U-shaped groove is provided on the top of the spherical rotating frame, and the UV curing lamp 105 is fixed in the U-shaped groove.

[0055] Embodiment 5:

[0056] The embodiment of the present invention provides an integrated microfluidic driving and real-time observation device, whose main structure is the same as that of Example 4, except that: this embodiment defines the support base of the microfluidic chip loading device.

[0057] Specifically, refer to Figure 5 In this embodiment, the support base includes an XY-axis displacement platform 101, a Z-axis displacement platform 102 and a connecting plate 103; the connecting plate 103 is installed on the top of the XY-axis displacement platform 101, and the Z-axis displacement platform 102 is installed on the connecting plate 103.

[0058] In this embodiment, an operation hole 532 is opened on the side wall of the housing 5, and the adjustment knobs of the XY-axis displacement platform 101 and the Z-axis displacement platform 102 pass through the operation hole 532 and extend to the outside of the housing 5 for easy external operation.

[0059] Embodiment 6:

[0060] The embodiment of the present invention provides an integrated microfluidic driving and real-time observation device, whose main structure is the same as that of embodiment 4, except that: this embodiment limits the structure of the chip mounting plate.

[0061] Specifically, refer to Fig. 9 In this embodiment, the chip mounting plate includes a fixing plate 108, two groups of chip fixing slides 109 and two groups of fixing bolts; the fixing plate 108 includes a central part and edge parts on both sides of the central part; the central part is provided with a first groove, the depth of the first groove is equal to or slightly higher than the thickness of the installed microfluidic chip; an opening for light transmission is provided in the middle position of the first groove, and the shape of the opening can be a notch shape, a circle, a square or other suitable shape; the edge part is provided with one or more than one group of horizontally penetrating second grooves, the chip fixing slide 109 is provided with a bump adapted to the size of the second groove, and the chip fixing slide 109 is slidably connected to the edge part of the fixing plate 108 through the second groove and the bump; the edge part is provided with a threaded hole, and the chip fixing slide 109 is provided with a through hole corresponding to the position of the threaded hole. When the chip fixing slide 109 slides to the fixed position, the chip fixing slide 109 is fixed by passing the fixing bolt through the through hole and being threadedly connected with the fixing plate 108.

[0062] When in use, place the microfluidic chip in the first groove, slide the two sets of chip fixing slides 109 inward and tighten the fixing bolts to fix the microfluidic chip installed on the chip mounting plate, loosen the fixing bolts and slide the two sets of chip fixing slides 109 outward to remove the microfluidic chip installed on the chip mounting plate.

[0063] Embodiment 7:

[0064] The embodiment of the present invention provides an integrated microfluidic driving and real-time observation device, whose main structure is the same as that of Example 6, except that: this embodiment limits the structure of the imaging component.

[0065] Specifically, refer to Figure 7In this embodiment, the imaging assembly includes a camera 301, a camera lens 302 and an adjustable support device. The adjustable support device includes a motor, a camera fixing frame 303, a lens support frame 304, a screw module 305, a slide rail 306, a slider 307 and a slide rail base 308; the screw module 305 includes a screw base, a screw and a slide seat, the screw base includes a bottom plate, one end of the bottom plate is provided with a vertical side plate, and the other end is installed with a motor; one end of the screw is rotatably connected to the side plate, and the other end is connected to the output end of the motor; the slide seat is threadedly connected to the screw, and the bottom of the slide seat is slidably connected to the screw base; the camera fixing frame 303 is fixedly connected to the slide seat, and the camera 301 is installed on the camera fixing frame 303; the slide rail 306 is fixedly installed on the slide rail base 308, the slider 307 is fixedly installed at the bottom of the lens support frame 304, the slider 307 is slidably connected to the slide rail 306, and the camera lens 302 is installed on the lens support frame 304. The center of the camera lens 302 is substantially consistent with the center of the microfluidic chip mounting plate, that is, the center positions of the camera lens 302, the microfluidic chip mounting plate and the flat white light are substantially consistent.

[0066] In this embodiment, the camera fixing frame 303 is an L-shaped fixing frame. The lens support frame 304 is flat as a whole, with a semicircular opening on the top, the size of the opening is equal to the diameter of the lens, the bottom is flush, and the two ends are slightly larger and have screw holes, and the two ends are fixed on the slider 307 by screws.

[0067] When in use, the motor drives the screw to rotate, driving the slide and the camera fixing frame 303 to move horizontally and linearly, and then driving the camera 301 and the camera lens 302 to move horizontally and linearly. At the same time, the slider 307 and the lens support frame 304 move horizontally and linearly along the slide rail 306 to keep the overall structure stable, thereby realizing the extension and retraction of the camera lens assembly.

[0068] Embodiment 8:

[0069] This embodiment takes the device of embodiment 7 as an example to prepare double emulsion droplets, including the following steps:

[0070] The first step is to connect the power supply of the device and turn on the power switch. The device automatically completes the power-on self-test and displays the power-on self-test result on the display touch screen 61.

[0071] The second step is to loosen the fixing screws on the chip fixing slider 109, slide the chip fixing slider 109 outward, insert the double emulsion microfluidic chip 110 into the first groove of the chip mounting plate, and then slide the two chip fixing sliders 109 inward and tighten the fixing screws. Loosen the screws on the side of the first horizontal bracket 107, and manually adjust the height of the first horizontal bracket 107 to a suitable height so that the center heights of the chip mounted on the chip mounting plate, the illumination light source 111, and the camera lens 302 are roughly in a straight line.

[0072] The third step is to install and fix the syringes containing the reagents required for the preparation of double emulsion droplets on three syringe pumps, connect the pipes between the syringes and the microfluidic chip interface, and place the droplet collection vessel under the microfluidic chip.

[0073] The fourth step is to set the flow rates of the three injection pumps on the control touch screen 62 according to specific needs, turn on the illumination light source 111 on the display touch screen 61, control the camera movement of the imaging system, and observe the real-time image on the display touch screen 61 until a clear image of the microfluidic chip appears.

[0074] The fifth step is to operate on the control touch screen 62 to start the injection pump according to the experimental sequence to prepare double emulsion droplets.

[0075] In the sixth step, if necessary, the UV curing lamp 105 can be turned on on the control touch screen 62, and the UV lamp bracket 104 can be adjusted so that the light spot of the UV curing lamp 105 is aligned with the chip outlet to perform photocuring on the generated droplets.

[0076] Step 7: After the droplet preparation is completed, the syringe pump is turned off on the control touch screen 62, the illumination light source 111 is turned off on the display touch screen 61, and the camera is turned off.

[0077] Step 8: Remove the connected pipes, loosen the screws on the chip fixing slide 109, slide the two chip fixing slides 109 outward, and remove the chip.

[0078] Step 9. Turn off the power of the device.

[0079] The above are only preferred implementations of the present invention. The protection scope of the present invention is not limited to the above embodiments. All technical solutions under the concept of the present invention belong to the protection scope of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications without departing from the principle of the present invention should be regarded as the protection scope of the present invention.

Claims

1. An integrated microfluidic drive and real-time observation device, characterized in that: include: A microfluidic chip loading device, an imaging component, a liquid driving component and a display interaction component; the microfluidic chip loading device, the imaging component, the liquid driving component and the display interaction component are integrated and installed inside a housing (5).

2. The integrated microfluidic driving and real-time observation device according to claim 1, characterized in that: The housing (5) comprises a shell body and a door; The shell body comprises a whole machine bottom plate (401), a back plate and a side wall plate (53); the hatch comprises a first hatch (51) and a second hatch (52), the first hatch (51) and the second hatch (52) are respectively rotatably connected to the back plate, and divide the shell body (5) into a first cabin body and a second cabin body; The microfluidic chip loading device is arranged inside the first cabin, and the imaging component and the liquid driving component are arranged inside the second cabin; the display interaction component includes a control touch screen (62) and a display touch screen (61), the control touch screen (62) is arranged inside the first cabin, and the display touch screen (61) is arranged on the outer wall of the second cabin door (52).

3. The integrated microfluidic driving and real-time observation device according to claim 2, characterized in that: A first cabin (9) is arranged inside the first cabin body, a second cabin (8) is arranged inside the second cabin body, and a partition plate is arranged between the first cabin (9) and the second cabin (8); The microfluidic chip loading device is arranged inside the first chamber (9), and the control touch screen (62) is installed on the whole machine bottom plate (401); A support plate is arranged in the second cabin (8), dividing the second cabin (8) into two layers, the imaging assembly is arranged in the upper layer and fixed on the support plate, and a power supply (701) and a control device are arranged in the lower layer of the second cabin (8); the liquid drive assembly includes one or more injection pumps, and the injection pumps are fixedly mounted on the outer surface of the second cabin (8) and / or the bottom plate (401) of the whole machine; The microfluidic chip in the microfluidic chip loading device corresponds to the position of the lens of the imaging component, and a camera extension opening (531) is provided on the partition plate, and the size of the camera extension opening (531) is larger than the size of the lens.

4. The integrated microfluidic driving and real-time observation device according to claim 3, characterized in that: The liquid drive assembly comprises a first injection pump (21), a second injection pump (22) and a third injection pump (23); The outer side wall of the second chamber (8) is provided with a middle layer plate (402); The first injection pump (21) is fixedly arranged on the top plate of the second compartment (8), the second injection pump (22) is fixedly arranged on the middle plate (402), and the third injection pump (23) is fixedly arranged on the bottom plate (401) of the whole machine.

5. The integrated microfluidic driving and real-time observation device according to claim 3, characterized in that: The second cabin (8) is of a rectangular structure, comprising a front lower support frame (404), a front upper support frame (406), a rear upper support frame (407) and a rear lower support frame (405); The rear upper support frame (407) and the rear lower support frame (405) are fixed on the back plate, the front lower support frame (404) is vertically fixed on the whole machine bottom plate (401), and the front upper support frame (406) is fixed on the front lower support frame (404); panels are arranged outside the front upper support frame (406) and the front lower support frame (404).

6. The integrated microfluidic driving and real-time observation device according to claim 1, characterized in that: The microfluidic chip loading device comprises a supporting base, a multifunctional bracket, a chip mounting plate, an ultraviolet curing lamp (105) and an illumination light source (111); the multifunctional bracket is mounted on the supporting base; the chip mounting plate, the ultraviolet curing lamp (105) and the illumination light source (111) are respectively mounted on the multifunctional bracket; The multifunctional bracket comprises a vertical rod (106), a first horizontal bracket (107) and a second horizontal bracket (112); the vertical rod (106) is fixed on a supporting base; the first horizontal bracket (107) is adjustably mounted on the vertical rod (106), and the chip mounting plate is fixedly mounted on the first horizontal bracket (107); the second horizontal bracket (112) is fixedly mounted on the bottom of the vertical rod (106), a UV lamp bracket (104) is fixedly arranged on the second horizontal bracket (112), and the UV curing lamp (105) is mounted on the UV lamp bracket (104); the lighting light source (111) is fixedly mounted on the vertical rod (106), and the lighting light source (111) is distributed behind the chip mounting plate.

7. The integrated microfluidic driving and real-time observation device according to claim 6, characterized in that: The first horizontal bracket (107) comprises a horizontal connecting piece and a U-shaped slider with a single-side opening; the U-shaped slider is slidably connected to the vertical rod (106), the horizontal connecting piece is fixed to one side of the slider, and the chip mounting plate is fixedly mounted on the horizontal connecting piece; a threaded hole is provided on the U-shaped slider, and the first horizontal bracket (107) is fixed by screwing a bolt fixing piece into the threaded hole and supporting the side wall of the vertical rod (106); The ultraviolet lamp bracket (104) comprises an L-shaped connecting rod and a spherical rotating frame; the L-shaped connecting rod is fixedly connected to the vertical rod (106), and the spherical rotating frame is fixedly connected to the L-shaped connecting rod; a U-shaped slot is provided on the top of the spherical rotating frame, and the ultraviolet curing lamp (105) is fixed in the U-shaped slot.

8. The integrated microfluidic driving and real-time observation device according to claim 6, characterized in that: The support base comprises an XY-axis displacement platform (101), a Z-axis displacement platform (102) and a connecting plate (103); The connecting plate (103) is mounted on the top of the XY-axis displacement platform (101), and the Z-axis displacement platform (102) is mounted on the connecting plate (103); An operating hole (532) is provided on the side wall of the shell (5), and the adjustment knobs of the XY-axis displacement platform (101) and the Z-axis displacement platform (102) pass through the operating hole (532) and extend to the outside of the shell (5).

9. The integrated microfluidic driving and real-time observation device according to claim 6, characterized in that: The chip mounting plate comprises a fixing plate (108), two groups of chip fixing slides (109) and two groups of fixing bolts; The fixing plate (108) comprises a central portion and edge portions on both sides of the central portion; The central portion is provided with a first groove adapted to the size of the microfluidic chip, and an opening for light transmission is provided in the middle of the first groove; The edge portion is provided with one or more than one set of horizontally penetrating second grooves, the chip fixing slide (109) is provided with a bump matched to the size of the second grooves, and the chip fixing slide (109) is slidably connected to the edge portion of the fixing plate (108) via the second grooves and the bumps; The edge portion is provided with a threaded hole, and the chip fixing slide (109) is provided with a through hole corresponding to the position of the threaded hole. When the chip fixing slide (109) slides to a fixed position, a fixing bolt passes through the through hole and is threadedly connected to the fixing plate (108) to fix the chip fixing slide (109).

10. The integrated microfluidic driving and real-time observation device according to claim 1, characterized in that: The imaging assembly comprises a camera (301), a camera lens (302) and an adjustable support device; The adjustable support device comprises a motor, a camera fixing frame (303), a lens supporting frame (304), a screw module (305), a slide rail (306), a slider (307) and a slide rail base (308); The screw module (305) comprises a screw base, a screw and a slide seat, wherein the screw base comprises a bottom plate, one end of the bottom plate is provided with a vertical side plate, and the other end is provided with a motor; one end of the screw is rotatably connected to the side plate, and the other end is connected to the output end of the motor; the slide seat is threadedly connected to the screw, and the bottom of the slide seat is slidably connected to the screw base; the camera fixing frame (303) is fixedly connected to the slide seat, and the camera (301) is installed on the camera fixing frame (303); The slide rail (306) is fixedly mounted on a slide rail base (308), the slider (307) is fixedly mounted on the bottom of the lens support frame (304), the slider (307) is slidably connected to the slide rail (306), and the camera lens (302) is mounted on the lens support frame (304).

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