Mixed silage method of silage soybeans and folium isatidis

Abstract

The invention belongs to the field of silage, and particularly discloses a silage soybean and folium isatidis mixed silage method which comprises the following steps: 1) respectively pretreating soybeans and folium isatidis, namely cleaning to remove soil impurities; 2) cleaning the ground and the wall surface of the silage silo with water, sterilizing and drying; 3) crushing the soybeans, cutting the folium isatidis into pieces with the length of 2-3cm, flatly laying on the ground of the silage silo, paving the crushed soybeans on the ground when the paving thickness is more than 10cm, then alternately paving the folium isatidis and the soybeans with the thickness of a soybean layer being 3-8 times of the thickness of a folium isatidis layer, and filling the folium isatidis at a position which is 5-10cm away from the soybeans and the wall surface of the silage silo; 4) spreading the folium isatidis for 10-20 cm on the uppermost layer, pressing a heavy object on the folium isatidis, and ensiling; and 5) during ensiling, controlling the temperature in the silage pit to be 30-40 DEG C, timely cleaning liquid at the bottom, and ensiling for 1-12 months to obtain the mixed silage.

Description

Technical Field

[0001] This invention belongs to the field of silage, and specifically discloses a method for silaging soybeans and Isatis tinctoria leaves. Background Art

[0002] Soybean silage is a feed made by harvesting soybean plants at specific growth stages and then processing them through specific fermentation and storage processes. This feed method has significant potential for application in large-scale livestock farming, especially in animals with high protein requirements. Soybean silage is rich in high-quality protein and fat, which helps improve animal production performance and health. It achieves effective utilization and conversion of soybean resources, reducing waste and increasing economic benefits. The quality and nutritional value of soybean silage can be optimized by adjusting factors such as harvesting timing and silage conditions. Soybean silage production requires advanced technology and strict control at every stage to ensure the quality and safety of the silage. During the silage process, some nutrients may be lost due to fermentation, especially sensitive components such as soluble carbohydrates and vitamins. Improper operation or poor management can easily lead to quality problems such as overheating, mold growth, and poor palatability, affecting the quality and utilization rate of the silage.

[0003] Currently, mastitis is a serious problem in dairy cows, and antibiotics are commonly used for treatment, leading to antibiotic overuse. Isatis tinctoria, a traditional Chinese medicine, has a long history and wide application value. However, the cultivation and processing of Isatis tinctoria into medicinal beverages often generates a lot of waste. Randomly discarding this waste pollutes the environment and causes waste. There are currently no reports of simultaneously ensiling Isatis tinctoria and soybeans as silage for dairy cows, which warrants further research. Summary of the Invention

[0004] To address the above problems, this invention discloses a method for ensiling soybeans and Isatis tinctoria leaves together.

[0005] The objective of this invention is achieved through the following technical solution.

[0006] A method for ensiling soybeans and Isatis tinctoria leaves together includes the following steps:

[0007] 1) Pre-treat soybeans and Chinese indigo leaves separately, including washing to remove soil and impurities; soybeans are usually harvested from the pod-setting stage to the early grain-filling stage, when the nutritional value of soybeans is high and they are easy to ensilage.

[0008] 2) Clean the floor and walls of the silage pit with water, sterilize them, and dry them;

[0009] 3) Crush the soybeans and chop the indigo leaves into 2-3cm lengths. Spread them evenly on the floor of the silage pit. When the spread thickness is more than 10cm, spread the soybeans on top. Then spread the indigo leaves and soybeans alternately. The thickness of the soybean layer is 3-8 times the thickness of the indigo leaf layer. Fill the gap between the soybeans and the walls of the silage pit with indigo leaves 5-10cm.

[0010] 4) Spread 10-20cm of indigo leaves on the top layer, press them down with heavy objects, and ensilage them.

[0011] 5) During silage, control the temperature inside the silage pit between 30-40℃, and clean up the liquid at the bottom in time. The silage time is 1-12 months to obtain mixed silage.

[0012] Furthermore, in the above-mentioned method for ensiling soybeans and Isatis tinctoria leaves, the moisture content of both soybeans and Isatis tinctoria leaves after pretreatment in step 1) is between 60-70%.

[0013] Furthermore, in the above-mentioned method for ensiling soybeans and Isatis tinctoria leaves, the sterilization in step 2) is ultraviolet sterilization, wherein the ultraviolet wavelength is 253.7 nm and the irradiation dose is greater than or equal to 15000 μW·s / cm. 2 .

[0014] Furthermore, in the above-mentioned method for ensiling soybeans and Isatis tinctoria leaves, in step 3), after the Isatis tinctoria leaves are spread and before the soybeans are spread, a compound microbial agent 1 is sprayed on the surface of the Isatis tinctoria leaves at a spraying rate of ≥10mL / m². 3 ;

[0015] The compound microbial agent 1 is composed of the fermentation broth of the following bacteria: Lactobacillus plantarum, Streptococcus lactis, Enterococcus faecalis, yeast, and Bacillus subtilis; the number of each of the above bacteria in each 1 mL of compound microbial agent 1 is not less than 10. 5 indivual.

[0016] Furthermore, in the above-mentioned method for ensiling soybeans and Isatis tinctoria leaves, in step 3), the soybeans are spread on the Isatis tinctoria leaves, and after each layer of soybeans is spread, a compound microbial agent 2 needs to be sprayed on the surface of the soybeans once, with a spraying amount of ≥10mL / m². 3 ;

[0017] The compound microbial agent 2 is composed of the fermentation broth of the following bacteria: Lactobacillus plantarum, Streptococcus lactis, Enterococcus faecalis, Lactobacillus casei, Lactobacillus brunelli, Lactobacillus brevis, and Rhodopseudomonas palustris; the number of each of the above bacteria in each 1 mL of compound microbial agent 1 is not less than 10. 5 indivual.

[0018] Furthermore, in the above-mentioned method for ensiling soybeans and Isatis tinctoria leaves, in step 3), after spraying the soybean surface with compound microbial agent 2 once, a layer of Chinese medicinal herb mixture is spread out.

[0019] Furthermore, in the above-mentioned method for ensiling soybeans and Isatis tinctoria leaves, the medicinal material mixture, calculated by weight, consists of the following medicinal materials:

[0020]

[0021]

[0022] Furthermore, in the above-mentioned method for ensiling soybeans and Isatis tinctoria leaves, the medicinal herb mixture layer is prepared by the following steps: the formula amounts of Astragalus membranaceus, Lycium barbarum, Angelica sinensis, jujube, hawthorn, honeysuckle, and Sophora flavescens are thoroughly mixed, then fed into a pulverizing device to be pulverized into powder, collected through a 100-mesh sieve, and the collected powder is spread on the surface of soybeans to a thickness of 2-3 cm.

[0023] The present invention also discloses a silage feed prepared by the method described in any of the above-mentioned methods.

[0024] The present invention also discloses the application of the above-mentioned silage in the preparation of dairy cow feed.

[0025] Compared with existing technologies, the present invention has the following advantages and beneficial effects:

[0026] This invention discloses a method for ensiling soybeans and Isatis tinctoria leaves together. Firstly, by precisely controlling the timing of harvesting and pre-treatment of soybeans and Isatis tinctoria leaves, the method ensures high nutritional value and favorable ensiling conditions, thereby improving the overall quality of the silage. Simultaneously, the cleaning and sterilization of the silage pit effectively prevents contamination by miscellaneous bacteria, ensuring hygiene and safety during the ensiling process. Secondly, the layered laying and spraying of a compound microbial agent not only promotes the fermentation process of soybeans and Isatis tinctoria leaves but also enhances the palatability and nutritional value of the feed. In particular, the selection of the compound microbial agent fully considers the synergistic effects of different microbial strains during fermentation, making the nutrients in the feed more comprehensive and easily absorbed and utilized by dairy cows. The Isatis tinctoria leaves of this invention have medicinal effects of clearing heat and detoxifying, reducing inflammation and fighting bacteria. As waste, they can be mixed with soybeans for ensiling and fermentation, effectively solving the problems of antibiotic abuse and high drug resistance in livestock feed. Furthermore, the addition of the mixed layer of traditional Chinese medicine herbs further enhances the unique health benefits of the silage. Traditional Chinese medicinal herbs such as astragalus and wolfberry are rich in various active ingredients, which can enhance the immunity and disease resistance of dairy cows, and improve milk yield and milk quality. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 The effect of mixed silage on milk yield (average) in dairy cows;

[0028] Figure 2 The effect of mixed silage on the milk quality (average) of dairy cows;

[0029] Figure 3 The effect of mixed silage on reducing mastitis in dairy cows. DETAILED DESCRIPTION

[0030] A method for ensiling soybeans and Isatis tinctoria leaves together includes the following steps:

[0031] 1) Pre-treat the soybeans and indigo leaves separately, including washing to remove soil and impurities;

[0032] 2) Clean the floor and walls of the silage pit with water, sterilize them, and dry them;

[0033] 3) Crush the soybeans and chop the indigo leaves into 2-3cm lengths. Spread them evenly on the floor of the silage pit. When the spread thickness is more than 10cm, spread the soybeans on top. Then spread the indigo leaves and soybeans alternately. The thickness of the soybean layer is 3-8 times the thickness of the indigo leaf layer. Fill the gap between the soybeans and the walls of the silage pit with indigo leaves 5-10cm apart to form a silage structure with indigo leaves around the perimeter and alternating layers of soybeans and indigo leaves in the middle.

[0034] 4) Spread 10-20cm of indigo leaves on the top layer, press them down with heavy objects, and ensilage them.

[0035] 5) During silage, control the temperature inside the silage pit between 30-40℃, clean up the liquid at the bottom in time, and the silage time is 1-12 months to obtain mixed silage.

[0036] After the pretreatment in step 1), the moisture content of both soybeans and Isatis indigotica leaves is between 60-70%.

[0037] The sterilization in step 2) is ultraviolet sterilization, with an ultraviolet wavelength of 253.7 nm and an irradiation dose greater than or equal to 15000 μW·s / cm. 2 .

[0038] In step 3), after the Isatis leaf is spread and before the soybean is spread, compound microbial agent 1 is sprayed on the surface of the Isatis leaf at a rate of ≥10mL / m. 3 ;

[0039] The compound microbial agent 1 is composed of the fermentation broth of the following bacteria: Lactobacillus plantarum, Streptococcus lactis, Enterococcus faecalis, yeast, and Bacillus subtilis; the number of each of the above bacteria in each 1 mL of compound microbial agent 1 is not less than 10. 5 indivual.

[0040] In step 3), the soybeans are spread on the Isatis leaf. After each layer of soybeans is spread, compound microbial agent 2 needs to be sprayed on the surface of the soybeans once, with a spraying amount of ≥10mL / m. 3 ;

[0041] The compound microbial agent 2 is composed of the fermentation broth of the following bacteria: Lactobacillus plantarum, Streptococcus lactis, Enterococcus faecalis, Lactobacillus casei, Lactobacillus brunelli, Lactobacillus brevis, and Rhodopseudomonas palustris; the number of each of the above bacteria in each 1 mL of compound microbial agent 1 is not less than 10. 5 indivual.

[0042] In step 3), after spraying the soybean surface with compound microbial agent 2 once, a layer of Chinese herbal medicine mixture is then spread.

[0043] The mixture of Chinese medicinal materials, calculated by weight, consists of the following medicinal materials:

[0044]

[0045]

[0046] The mixture of Chinese medicinal materials is prepared by the following steps: the prescribed amounts of Astragalus membranaceus, Lycium barbarum, Angelica sinensis, jujube, hawthorn, honeysuckle, and Sophora flavescens are thoroughly mixed and then fed into a pulverizing device to be pulverized into powder. The powder is then collected by passing it through a 100-mesh sieve and spread on the surface of soybeans to a thickness of 2-3 cm.

[0047] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to the accompanying drawings and embodiments. However, it should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the scope of the invention. Furthermore, descriptions of well-known structures and technologies are omitted in the following description to avoid unnecessarily obscuring the concept of the invention.

[0048] It should be noted that, unless otherwise specified, the embodiments and features described in this application can be combined with each other. The present invention will now be described in detail with reference to the embodiments.

[0049] Example 1

[0050] A method for ensiling soybeans and Isatis tinctoria leaves together includes the following steps:

[0051] 1) Pre-treat the soybeans and indigo leaves separately, including washing to remove soil and impurities;

[0052] 2) Clean the floor and walls of the silage pit with water, sterilize them, and dry them;

[0053] 3) Crush the soybeans and chop the indigo leaves into 2-3cm lengths. Spread them evenly on the floor of the silage pit. When the spread thickness is more than 10cm, spread the soybeans on top. Then spread the indigo leaves and soybeans alternately. The thickness of the soybean layer is 3-8 times the thickness of the indigo leaf layer. Fill the gap between the soybeans and the walls of the silage pit with indigo leaves 5-10cm apart to form a silage structure with indigo leaves around the perimeter and alternating layers of soybeans and indigo leaves in the middle.

[0054] 4) Spread 10-20cm of indigo leaves on the top layer, press them down with heavy objects, and ensilage them.

[0055] 5) During silage, control the temperature inside the silage pit between 30-40℃, clean up the liquid at the bottom in time, and the silage time is 1-12 months to obtain mixed silage.

[0056] After the pretreatment in step 1), the moisture content of both soybeans and Isatis indigotica leaves is between 60-70%.

[0057] The sterilization in step 2) is ultraviolet sterilization, with an ultraviolet wavelength of 253.7 nm and an irradiation dose greater than or equal to 15000 μW·s / cm. 2 .

[0058] In step 3), after the Isatis leaf is spread and before the soybean is spread, compound microbial agent 1 is sprayed on the surface of the Isatis leaf at a rate of ≥10mL / m. 3 ;

[0059] The compound microbial agent 1 is composed of the fermentation broth of the following bacteria: Lactobacillus plantarum, Streptococcus lactis, Enterococcus faecalis, yeast, and Bacillus subtilis; the number of each of the above bacteria in each 1 mL of compound microbial agent 1 is not less than 10. 5 indivual.

[0060] In step 3), the soybeans are spread on the Isatis leaf. After each layer of soybeans is spread, compound microbial agent 2 needs to be sprayed on the surface of the soybeans once, with a spraying amount of ≥10mL / m. 3 ;

[0061] The compound microbial agent 2 is composed of the fermentation broth of the following bacteria: Lactobacillus plantarum, Streptococcus lactis, Enterococcus faecalis, Lactobacillus casei, Lactobacillus brunelli, Lactobacillus brevis, and Rhodopseudomonas palustris; the number of each of the above bacteria in each 1 mL of compound microbial agent 1 is not less than 10. 5 indivual.

[0062] In step 3), after spraying the soybean surface with compound microbial agent 2 once, a layer of Chinese herbal medicine mixture is then spread.

[0063] The mixture of Chinese medicinal materials, calculated by weight, consists of the following medicinal materials:

[0064]

[0065] The mixture of Chinese medicinal materials is prepared by the following steps: the prescribed amounts of Astragalus membranaceus, Lycium barbarum, Angelica sinensis, jujube, hawthorn, honeysuckle, and Sophora flavescens are thoroughly mixed and then fed into a pulverizing device to be pulverized into powder. The powder is then collected by passing it through a 100-mesh sieve and spread on the surface of soybeans to a thickness of 2-3 cm.

[0066] Example 2

[0067] A method for ensiling soybeans and Isatis tinctoria leaves together includes the following steps:

[0068] 1) Pre-treat the soybeans and indigo leaves separately, including washing to remove soil and impurities;

[0069] 2) Clean the floor and walls of the silage pit with water, sterilize them, and dry them;

[0070] 3) Crush the soybeans and chop the indigo leaves into 2-3cm lengths. Spread them evenly on the floor of the silage pit. When the spread thickness is more than 10cm, spread the soybeans on top. Then spread the indigo leaves and soybeans alternately. The thickness of the soybean layer is 3-8 times the thickness of the indigo leaf layer. Fill the gap between the soybeans and the walls of the silage pit with indigo leaves 5-10cm apart to form a silage structure with indigo leaves around the perimeter and alternating layers of soybeans and indigo leaves in the middle.

[0071] 4) Spread 10-20cm of indigo leaves on the top layer, press them down with heavy objects, and ensilage them.

[0072] 5) During silage, control the temperature inside the silage pit between 30-40℃, clean up the liquid at the bottom in time, and the silage time is 1-12 months to obtain mixed silage.

[0073] After the pretreatment in step 1), the moisture content of both soybeans and Isatis indigotica leaves is between 60-70%.

[0074] The sterilization in step 2) is ultraviolet sterilization, with an ultraviolet wavelength of 253.7 nm and an irradiation dose greater than or equal to 15000 μW·s / cm. 2 .

[0075] In step 3), after the Isatis leaf is spread and before the soybean is spread, compound microbial agent 1 is sprayed on the surface of the Isatis leaf at a rate of ≥10mL / m. 3 ;

[0076] The compound microbial agent 1 is composed of the fermentation broth of the following bacteria: Lactobacillus plantarum, Streptococcus lactis, Enterococcus faecalis, yeast, and Bacillus subtilis; the number of each of the above bacteria in each 1 mL of compound microbial agent 1 is not less than 10. 5 indivual.

[0077] In step 3), the soybeans are spread on the Isatis leaf. After each layer of soybeans is spread, compound microbial agent 2 needs to be sprayed on the surface of the soybeans once, with a spraying amount of ≥10mL / m. 3 ;

[0078] The compound microbial agent 2 is composed of the fermentation broth of the following bacteria: Lactobacillus plantarum, Streptococcus lactis, Enterococcus faecalis, Lactobacillus casei, Lactobacillus brunelli, Lactobacillus brevis, and Rhodopseudomonas palustris; the number of each of the above bacteria in each 1 mL of compound microbial agent 1 is not less than 10. 5 indivual.

[0079] In step 3), after spraying the soybean surface with compound microbial agent 2 once, a layer of Chinese herbal medicine mixture is then spread.

[0080] The mixture of Chinese medicinal materials, calculated by weight, consists of the following medicinal materials:

[0081]

[0082] The mixture of Chinese medicinal materials is prepared by the following steps: the prescribed amounts of Astragalus membranaceus, Lycium barbarum, Angelica sinensis, jujube, hawthorn, honeysuckle, and Sophora flavescens are thoroughly mixed and then fed into a pulverizing device to be pulverized into powder. The powder is then collected by passing it through a 100-mesh sieve and spread on the surface of soybeans to a thickness of 2-3 cm.

[0083] Example 3

[0084] A method for ensiling soybeans and Isatis tinctoria leaves together includes the following steps:

[0085] 1) Pre-treat the soybeans and indigo leaves separately, including washing to remove soil and impurities;

[0086] 2) Clean the floor and walls of the silage pit with water, sterilize them, and dry them;

[0087] 3) Crush the soybeans and chop the indigo leaves into 2-3cm lengths. Spread them evenly on the floor of the silage pit. When the spread thickness is more than 10cm, spread the soybeans on top. Then spread the indigo leaves and soybeans alternately. The thickness of the soybean layer is 3-8 times the thickness of the indigo leaf layer. Fill the gap between the soybeans and the walls of the silage pit with indigo leaves 5-10cm apart to form a silage structure with indigo leaves around the perimeter and alternating layers of soybeans and indigo leaves in the middle.

[0088] 4) Spread 10-20cm of indigo leaves on the top layer, press them down with heavy objects, and ensilage them.

[0089] 5) During silage, control the temperature inside the silage pit between 30-40℃, clean up the liquid at the bottom in time, and the silage time is 1-12 months to obtain mixed silage.

[0090] After the pretreatment in step 1), the moisture content of both soybeans and Isatis indigotica leaves is between 60-70%.

[0091] The sterilization in step 2) is ultraviolet sterilization, with an ultraviolet wavelength of 253.7 nm and an irradiation dose greater than or equal to 15000 μW·s / cm. 2 .

[0092] In step 3), after the Isatis leaf is spread and before the soybean is spread, compound microbial agent 1 is sprayed on the surface of the Isatis leaf at a rate of ≥10mL / m. 3 ;

[0093] The compound microbial agent 1 is composed of the fermentation broth of the following bacteria: Lactobacillus plantarum, Streptococcus lactis, Enterococcus faecalis, yeast, and Bacillus subtilis; the number of each of the above bacteria in each 1 mL of compound microbial agent 1 is not less than 10. 5 indivual.

[0094] In step 3), the soybeans are spread on the Isatis leaf. After each layer of soybeans is spread, compound microbial agent 2 needs to be sprayed on the surface of the soybeans once, with a spraying amount of ≥10mL / m. 3 ;

[0095] The compound microbial agent 2 is composed of the fermentation broth of the following bacteria: Lactobacillus plantarum, Streptococcus lactis, Enterococcus faecalis, Lactobacillus casei, Lactobacillus brunelli, Lactobacillus brevis, and Rhodopseudomonas palustris; the number of each of the above bacteria in each 1 mL of compound microbial agent 1 is not less than 10. 5 indivual.

[0096] In step 3), after spraying the soybean surface with compound microbial agent 2 once, a layer of Chinese herbal medicine mixture is then spread.

[0097] The mixture of Chinese medicinal materials, calculated by weight, consists of the following medicinal materials:

[0098]

[0099]

[0100] The mixture of Chinese medicinal materials is prepared by the following steps: the prescribed amounts of Astragalus membranaceus, Lycium barbarum, Angelica sinensis, jujube, hawthorn, honeysuckle, and Sophora flavescens are thoroughly mixed and then fed into a pulverizing device to be pulverized into powder. The powder is then collected by passing it through a 100-mesh sieve and spread on the surface of soybeans to a thickness of 2-3 cm.

[0101] Comparative Example 1

[0102] The compound bacterial agent 2 is not contained, and the compound bacterial agent 1 is used to replace the function of the compound bacterial agent 2. Other steps are the same as those in Example 2.

[0103] Comparative Example 2

[0104] It does not contain compound microbial agents 1 and 2, and is otherwise the same as in Example 2.

[0105] Comparative Example 3

[0106] It does not contain a mixture of Chinese medicinal herbs; otherwise, it is the same as in Example 2.

[0107] Comparative Example 4

[0108] It does not contain Isatis tinctoria leaves, only uses soybean silage, and does not contain compound microbial agent 1. Otherwise, it is the same as in Example 2. Test Example 1

[0109] Verify the application of the mixed silage in Examples 1-3 and Comparative Examples 1-4 in dairy cow feed.

[0110] The experiment was conducted on a dairy farm. Twenty Holstein cows (700±50 kg weight, lactation period 150±5 days) were randomly selected for each group. The total mixed diet (TMR) for the treatment and control groups is shown in Table 1. The cows were housed in separate free-range pens. The feeding period was set at 60 days. Milk samples were collected on days 15, 30, and 60 for inter-group analysis. The results are shown in Table 2. The results indicate that the formulation with added mixed silage improved milk yield and milk quality.

[0111] Table 1 Total Mixed Diet Formulas for Treatment and Control Groups

[0112] Treatment group wt% control group wt% hay 10％ 10％ Flake corn 20％ 40％ High-yield high-quality grain 40％ 40％ water 10％ 10％ Mixed silage 20％ 0％

[0113] Table 2 shows the impact on the average daily milk yield of dairy cows.

[0114]

[0115] From Table 2 and Figure 1 It can be seen that the mixed silage prepared by using the composite bacterial agents 1 and 2, and the solution with the addition of the mixture of Isatis indigotica and Chinese medicinal materials, has the best milk production after dairy cows consume it, which is higher than that of comparative examples 1-4 and significantly better than the control example.

[0116] Table 3. Effects on milk quality (protein content) in dairy cows

[0117] Protein content (wt%) (30 days) Protein content (wt%) (60 days) Example 1 3.35 3.42 Example 2 3.38 3.45 Example 3 3.41 3.53 Comparative Example 1 3.15 3.17 Comparative Example 2 3.13 3.15 Comparative Example 3 3.18 3.21 Comparative Example 4 3.12 3.15 Comparison Example 3.03 3.11

[0118] From Table 3 and Figure 2 It can be seen that the mixed silage prepared using the composite bacterial agents 1 and 2, and the solution with the addition of the mixture of Isatis indigotica and Chinese medicinal materials, has the best milk protein content after being consumed by dairy cows, which is higher than that of comparative examples 1-4 and significantly better than the control example.

[0119] Furthermore, the incidence of mastitis in dairy cows over 60 days was statistically analyzed. Specifically, the number of times mastitis occurred in each group and treatment intervention was accumulated. The results are shown in Table 4.

[0120] Table 4. Number of interventions required for mastitis during the feeding process (cumulative value for 20 cows per group over 60 days)

[0121]

[0122] From Table 4 and Figure 3 It can be seen that the mixed silage prepared by adding Daqingye (Isatis tinctoria leaf) and a mixture of traditional Chinese medicine significantly improved the symptoms of mastitis in dairy cows after they consumed it.

[0123] As can be seen from the above embodiments, this invention discloses a method for ensiling soybeans and Isatis tinctoria leaves together. Firstly, by precisely controlling the timing of harvesting and pre-treatment of soybeans and Isatis tinctoria leaves, this method ensures high nutritional value and favorable ensiling conditions for the raw materials, thereby improving the overall quality of the ensiling feed. Simultaneously, the cleaning and sterilization of the ensiling pit effectively prevents contamination by miscellaneous bacteria, ensuring hygiene and safety during the ensiling process. Secondly, the layered laying and spraying of compound microbial agents not only promotes the fermentation process of soybeans and Isatis tinctoria leaves but also enhances the palatability and nutritional value of the feed. In particular, the selection of the compound microbial agent fully considers the synergistic effects of different microbial strains during fermentation, making the nutrients in the feed more comprehensive and easier for dairy cows to absorb and utilize. The Isatis tinctoria leaves of this invention have medicinal effects of clearing heat and detoxifying, reducing inflammation and fighting bacteria. As waste, they can be mixed with soybeans for ensiling and fermentation, effectively solving the problems of antibiotic abuse and high drug resistance in livestock feed. Furthermore, the addition of the mixed layer of traditional Chinese medicine materials adds unique health benefits to the ensiling feed. Traditional Chinese medicinal herbs such as astragalus and wolfberry are rich in various active ingredients, which can enhance the immunity and disease resistance of dairy cows, and improve milk yield and milk quality.

[0124] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Therefore, any changes and modifications made to the embodiments described herein based on the innovative concept of the present invention, or equivalent structural or procedural transformations made using the content of the present invention's specification and drawings, directly or indirectly applying the above technical solutions to other related technical fields, are all included within the scope of protection of this patent.

Claims

1. A method for mixed silage of soybean silage and Folium Isatidis, It is characterized in that The following steps are involved: 1) pre-treating soybeans and isatis indigofera folium separately, including washing to remove soil impurities; 2) Clean the floor and walls of the silo with water, sterilize and dry them; 3) Crush the soybeans, chop the isatis indigo leaves into pieces of 2-3 cm in length, and spread them flat on the ground of the silo. When the paving thickness is more than 10 cm, spread the crushed soybeans on it, and then spread the isatis indigo leaves and soybeans alternately. The thickness of the soybean layer is 3-8 times the thickness of the isatis indigo leaf layer, and fill the isatis indigo leaves 5-10 cm between the soybeans and the silo wall; 4) On the top layer, spread 10-20 cm of Isatis indigo leaves and put heavy objects on them for silage; 5) During the silage period, the temperature in the silo is controlled between 30-40°C, and the liquid at the bottom is cleaned in time. The silage time is 1-12 months to obtain mixed silage.

2. A method for mixed silage of soybean silage and Folium Isatidis according to claim 1, It is characterized in that After the pretreatment in step 1), the moisture content of soybean and Folium Isatidis is between 60% and 70%.

3. A method for mixed silage of soybean silage and Folium Isatidis according to claim 1, It is characterized in that The sterilization in step 2) is ultraviolet sterilization, the ultraviolet wavelength is 253.7nm, and the irradiation dose is greater than or equal to 15000μW·s / cm 2 .

4. The method for mixed silage of soybean silage and Folium Isatidis according to claim 1, It is characterized in that In the step 3), after the folium isatidis is spread and before the soybean is spread, the composite bacterial agent 1 is sprayed on the surface of the folium isatidis, and the spraying amount is ≥10mL / m 3 ; The composite bacterial agent 1 is a mixture of fermented broths of the following bacteria: Lactobacillus plantarum, Streptococcus lactis, Streptococcus faecalis, yeast and Bacillus subtilis; the number of each of the above bacteria in each mL of the composite bacterial agent 1 is not less than 10 5 indivual.

5. The method for mixed silage of soybean silage and Folium Isatidis according to claim 1, It is characterized in that In the step 3), soybeans are spread on the isatis indigo leaves. Each time the soybean layer is spread, the composite bacterial agent 2 needs to be sprayed on the soybean surface once, and the spraying amount is ≥10mL / m 3 ; The composite bacterial agent 2 is a mixture of fermented broths of the following bacteria: Lactobacillus plantarum, Streptococcus lactis, Streptococcus faecalis, Lactobacillus casei, Lactobacillus brunneri, Lactobacillus brevis and Rhodopseudomonas palustris; the number of each of the above bacteria in each 1 mL of the composite bacterial agent 1 is not less than 10 5 indivual.

6. A method for mixed silage of soybean silage and Folium Isatidis according to claim 5, It is characterized in that In the step 3), after the composite bacterial agent 2 is sprayed once on the soybean surface, a layer of the Chinese medicinal material mixture is spread.

7. A method for mixed silage of soybean silage and Folium Isatidis according to claim 6, It is characterized in that The Chinese medicinal material mixture is composed of the following medicinal materials by weight:

8. The method for mixed silage of soybean silage and Folium Isatidis according to claim 7, It is characterized in that The Chinese medicinal material mixture layer is prepared by the following steps: after fully mixing the Chinese medicinal drinks of astragalus, wolfberry, angelica, red dates, hawthorn, honeysuckle and sophora flavescens in the formula amount, entering into a crushing device, crushing into powder, collecting through a 100-mesh sieve, and spreading the collected powder on the surface of soybeans with a spreading thickness of 2-3 cm.

9. A kind of silage, It is characterized in that Prepared by the method according to any one of claims 1 to 8.

10. Use of the silage according to claim 9 in preparing dairy cow feed.

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