Veterinary traditional Chinese medicine granule for preventing and treating poultry renal infectious bronchitis and application of veterinary traditional Chinese medicine granule

Abstract

The invention relates to the technical field of traditional Chinese medicine preparations, and provides veterinary traditional Chinese medicine granules for preventing and treating poultry renal infectious bronchitis and application of the veterinary traditional Chinese medicine granules. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 20 to 40 parts of herba pogostemonis, 40 to 50 parts of herba artemisiae scopariae, 30 to 50 parts of radix isatidis, 40 to 60 parts of rhizoma atractylodis macrocephalae, 50 to 70 parts of polyporus umbellatus, 40 to 60 parts of rhizoma alismatis, 30 to 40 parts of pericarpium citri reticulatae, 10 to 30 parts of radix sophorae flavescentis, 30 to 50 parts of radix platycodonis, 50 to 70 parts of radix scutellariae, 10 to 30 parts of herba polygoni avicularis, 30 to 50 parts of stir-fried rhizoma dioscoreae and 10 to 30 parts of radix glycyrrhizae preparata. According to the technical scheme, the problems of high incidence rate and high death rate of poultry renal infectious bronchitis in related technologies are solved.

Description

Technical Field

[0001] The present invention relates to the technical field of Chinese medicine preparations, and in particular to a Chinese medicine granule for preventing and treating poultry renal bronchitis and an application thereof. Background Art

[0002] In the poultry farming industry, nephrotic bronchitis has always been one of the major diseases that troubles farmers. Nephrotic infectious bronchitis is an acute, highly contagious disease caused by avian infectious bronchitis virus, which mainly attacks the kidneys and other organs of poultry. Severe nephrotic infectious bronchitis can lead to a high mortality rate, especially in young poultry.

[0003] In recent years, with the continuous expansion of poultry farming and the improvement of intensiveness, the incidence of renal tuberculosis has also shown an upward trend. Traditional prevention and treatment methods mainly rely on chemical drugs and vaccines, but the long-term use of chemical drugs can easily lead to problems such as increased bacterial resistance and drug residues, which not only affects the quality and safety of poultry products, but also causes certain pollution to the environment. Although vaccines can prevent the occurrence of renal tuberculosis to a certain extent, due to the rapid mutation of the virus, the protective effect of the vaccine is sometimes not ideal. At the same time, with people's pursuit of green, safe and residue-free breeding concepts, the advantages of veterinary Chinese medicine in the prevention and treatment of poultry diseases have gradually become prominent. Chinese medicine has the characteristics of being natural, safe, and having few side effects. Its mechanism of action is often multi-target and overall regulation, which can improve the immunity of poultry and enhance the body's resistance to disease. Therefore, it is of great practical significance to develop a veterinary Chinese medicine granule for the prevention and treatment of poultry renal tuberculosis. Summary of the invention

[0004] The present invention provides a veterinary Chinese medicine granule for preventing and treating poultry renal tracheitis and application thereof, which has the effects of warming yang and transforming qi, promoting dampness and promoting water circulation, clearing the lungs and resolving phlegm, and solves the problems of high incidence and high mortality of poultry renal tracheitis in the related art.

[0005] The technical solution of the present invention is as follows: The invention provides a veterinary Chinese medicine granule for preventing and treating poultry renal bronchitis. The raw materials include the following components in parts by weight: 20-40 parts of patchouli, 40-50 parts of wormwood, 30-50 parts of isatis root, 40-60 parts of atractylodes, 50-70 parts of umbellatus, 40-60 parts of oriental rhizome, 30-40 parts of tangerine peel, 10-30 parts of sophora flavescens, 30-50 parts of platycodon, 50-70 parts of scutellaria baicalensis, 10-30 parts of polygala polyphylla, 30-50 parts of stir-fried yam and 10-30 parts of roasted liquorice.

[0006] As a further technical solution, the raw materials include the following components in parts by weight: 30-35 parts of patchouli, 45-50 parts of wormwood, 35-45 parts of isatis root, 50-55 parts of atractylodes, 60-65 parts of umbellatus, 50-55 parts of oriental rhizome, 30-35 parts of tangerine peel, 20-25 parts of sophora flavescens, 35-45 parts of platycodon, 55-65 parts of scutellaria baicalensis, 15-25 parts of polygala japonica, 35-45 parts of stir-fried yam, and 20-30 parts of roasted licorice.

[0007] This scheme also proposes a method for preparing a veterinary Chinese medicine granule for preventing and treating poultry renal bronchitis, comprising the following steps: S1. Weigh the above raw materials, add them into water, soak, decoct, filter to obtain a decoction, and then concentrate and filter to obtain an extract; S2, mixing the extract and auxiliary materials uniformly, granulating, drying, and granulating to obtain a granule semi-finished product, and packaging to obtain a veterinary Chinese medicine granule for preventing and treating poultry renal bronchitis; The auxiliary materials include powdered sugar and water-melted realgar.

[0008] As a further technical solution, polyethylene glycol is also added during the soaking, and the mass ratio of the water to the polyethylene glycol is 20-16:1.

[0009] In the present invention, polyethylene glycol with different molecular weights is added as one of the extractants to broaden the solubility range of the extractant for various effective ingredients, improve the comprehensiveness of extraction, optimize the extraction process, thereby improving the efficiency and quality of traditional Chinese medicine extraction, strengthening the preventive and therapeutic effects of the medicine, and reducing the morbidity and mortality of poultry nephrotic infectious bronchitis.

[0010] In the present invention, the extractant is added twice to gradually infiltrate the Chinese medicinal raw materials, so that the tissues of the medicinal materials are gradually relaxed, which is beneficial to the subsequent release of effective ingredients, reduces the extraction of impurities, improves the quality of the extract, enhances the prevention and treatment effects of the drug, and reduces the morbidity and mortality of poultry renal bronchitis.

[0011] As a further technical solution, the polyethylene glycol consists of a first polyethylene glycol and a second polyethylene glycol, and the molecular weight of the first polyethylene glycol is smaller than the molecular weight of the second polyethylene glycol.

[0012] As a further technical solution, the molecular weight of the first polyethylene glycol is 270-330, the molecular weight of the second polyethylene glycol is 720-880, and the mass ratio of the first polyethylene glycol to the second polyethylene glycol is 2:2-3.

[0013] As a further technical solution, the decoction is carried out twice, the first decoction time is 1.5-2.5 hours, and the second decoction time is 0.5-1.5 hours.

[0014] As a further technical solution, the concentration is carried out twice, the drug content of the first concentrated clear paste is 1.08-1.12 g / mL, and the drug content of the second concentrated extract is 1.28-1.32 g / mL.

[0015] As a further technical solution, the mass ratio of the extract, powdered sugar and water-melted realgar is 1:5:0.01.

[0016] The present invention also provides the application of the Chinese herbal granules for preventing and treating poultry renal herpes simplex disease or the Chinese herbal granules for preventing and treating poultry renal herpes simplex disease prepared by the preparation method in preventing and treating poultry renal herpes simplex disease.

[0017] The working principle and beneficial effects of the present invention are: In the present invention, water and polyethylene glycol are added as extractants at the same time, and the synergistic effect of the two is fully exerted, the diffusion and dissolution of the effective ingredients of the Chinese medicine raw materials are promoted, and a more stable environment is provided for the effective ingredients. By adjusting the mass ratio of the two, a more suitable concentration gradient is formed between the extract and the Chinese medicine raw materials, the viscosity of the extract is adjusted, the penetration speed and diffusion capacity of the extract are affected, the overall efficacy of the medicine is enhanced, the preventive and therapeutic effects of the medicine are strengthened, and the morbidity and mortality of poultry renal bronchitis are reduced. DETAILED DESCRIPTION

[0018] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0019] In the following examples and comparative examples: The first polyethylene glycol: molecular weight is 270~330, hydroxyl value is 340~416mgKOH / g, the manufacturer is Shanghai Lianji Chemical Co., Ltd.; The second polyethylene glycol: molecular weight is 720~880, hydroxyl value is 127~156mgKOH / g, and the manufacturer is Shanghai Lianji Chemical Co., Ltd.

[0020] Example 1 The preparation method of the extract comprises the following steps: S1, weigh 30 parts of patchouli, 45 parts of wormwood, 35 parts of isatis root, 50 parts of atractylodes, 60 parts of umbellatus, 50 parts of oriental rhizome, 30 parts of dried orange peel, 20 parts of sophora flavescens, 35 parts of platycodon, 55 parts of scutellaria, 15 parts of polysaccharides, 35 parts of fried yam, 20 parts of moxibustion liquorice, put them into a multifunctional extraction tank, add 3720 parts of extraction water and 148.8 parts of the first polyethylene glycol, soak for 3.5h, boil and extract for 1.5h, filter to obtain the first decoction; add 2790 parts of extraction water again, boil and extract for 0.5h, filter to obtain the second decoction; decompress the first decoction and the second decoction for the first time, concentrate to a drug content of about 1.08g / mL, obtain a clear paste, concentrate for the second time to a drug content of about 1.28g / mL, filter with a 300-mesh oscillating sieve to remove the charred residue, and obtain an extract; S2. Put the extract, powdered sugar and water-frozen realgar in a mass ratio of 1:5:0.01 into a wet mixer and mix them evenly to prepare an ointment. After granulation, drying and granulation, a semi-finished granule product is obtained. After packaging, a veterinary Chinese medicine granule for preventing and treating poultry renal bronchitis is obtained.

[0021] Example 2 The preparation method of the extract comprises the following steps: S1, weigh 32 parts of patchouli, 48 parts of wormwood, 40 parts of isatis root, 52 parts of atractylodes, 64 parts of umbellatus, 52 parts of oriental rhizome, 32 parts of dried orange peel, 24 parts of sophora flavescens, 40 parts of platycodon, 60 parts of scutellaria baicalensis, 20 parts of polysaccharides polyphylla, 40 parts of fried yam, 25 parts of moxibustion liquorice, put them into a multifunctional extraction tank, add 4240 parts of extraction water and 169.6 parts of the first polyethylene glycol, soak for 4h, boil and decoct for 2h, and filter to obtain the first decoction; add 3180 parts of extraction water again, boil and decoct for 1h, and filter to obtain the second decoction; decompress the first decoction and the second decoction for the first time, concentrate to a drug content of about 1.1g / mL, and obtain a clear paste, concentrate for the second time to a drug content of about 1.3g / mL, filter with a 300-mesh oscillating sieve to remove the charred residue, and obtain an extract; S2. Put the extract, powdered sugar and water-frozen realgar in a mass ratio of 1:5:0.01 into a wet mixer and mix them evenly to prepare an ointment. After granulation, drying and granulation, a semi-finished granule product is obtained. After packaging, a veterinary Chinese medicine granule for preventing and treating poultry renal bronchitis is obtained.

[0022] Example 3 The preparation method of the extract comprises the following steps: S1, weigh 35 parts of patchouli, 50 parts of wormwood, 45 parts of isatis root, 55 parts of atractylodes, 65 parts of umbellatus, 55 parts of oriental rhizome, 35 parts of dried orange peel, 25 parts of sophora flavescens, 45 parts of platycodon, 65 parts of scutellaria baicalensis, 25 parts of polysaccharides polyphylla, 45 parts of fried yam, 30 parts of moxibustion liquorice, put them into a multifunctional extraction tank, add 4600 parts of extraction water and 184 parts of the first polyethylene glycol, soak for 4.5h, boil and decoct for 2.5h, and filter to obtain the first decoction; add 3450 parts of extraction water again, boil and decoct for 1.5h, and filter to obtain the second decoction; decompress the first decoction and the second decoction for the first time, concentrate to a drug content of about 1.12g / mL, and obtain a clear paste, concentrate for the second time to a drug content of about 1.32g / mL, filter with a 300-mesh oscillating sieve to remove the charred residue, and obtain an extract; S2. Put the extract, powdered sugar and water-frozen realgar in a mass ratio of 1:5:0.01 into a wet mixer and mix them evenly to prepare an ointment. After granulation, drying and granulation, a semi-finished granule product is obtained. After packaging, a veterinary Chinese medicine granule for preventing and treating poultry renal bronchitis is obtained.

[0023] Example 4 Compared with Example 2, Example 4 is different in that 169.6 parts of the first polyethylene glycol are replaced by 212 parts of the first polyethylene glycol.

[0024] Example 5 Compared with Example 2, the difference in Example 5 is that 169.6 parts of the first polyethylene glycol are replaced by 265 parts of the first polyethylene glycol.

[0025] Example 6 Compared with Example 2, Example 6 is different in that 169.6 parts of the first polyethylene glycol are replaced by 280 parts of the first polyethylene glycol.

[0026] Example 7 Compared with Example 5, the difference of Example 7 is that 265 parts of the first polyethylene glycol are replaced by an equal amount of the second polyethylene glycol.

[0027] Example 8 Compared with Example 5, Example 8 is different in that 265 parts of the first polyethylene glycol are replaced by 150 parts of the first polyethylene glycol and 115 parts of the first polyethylene glycol.

[0028] Example 9 Compared with Example 5, the difference in Example 9 is that 265 parts of the first polyethylene glycol are replaced by 132.5 parts of the first polyethylene glycol and 132.5 parts of the first polyethylene glycol.

[0029] Example 10 Compared with Example 5, Example 10 is different in that 265 parts of the first polyethylene glycol are replaced by 106 parts of the first polyethylene glycol and 159 parts of the first polyethylene glycol.

[0030] Embodiment 11 Compared with Example 5, Example 11 is different in that 265 parts of the first polyethylene glycol are replaced by 100 parts of the first polyethylene glycol and 165 parts of the first polyethylene glycol.

[0031] Comparative Example 1 Compared with Example 2, Comparative Example 1 is different in that the first polyethylene glycol is not added.

[0032] Test 1 High performance liquid chromatography was used to detect the contents of hesperidin and matrine in the veterinary Chinese medicine granules prepared in Examples 1 to 11 and Comparative Example 1. The detection method refers to the 2020 edition of the Chinese Pharmacopoeia "Pharmacopoeia of the People's Republic of China".

[0033] Table 1 Test results of the contents of various components of Chinese veterinary medicine granules in Examples 1 to 11 and Comparative Example 1

[0034] Compared with Comparative Example 1, Example 2 simultaneously adds extraction water and polyethylene glycol as extractants, and the results show that the hesperidin and matrine contents of Example 2 are greater than those of Comparative Example 1, indicating that the extraction water and polyethylene glycol play a synergistic role and can improve the effective extraction of Chinese medicine components. In Examples 2 and Examples 4 to 6, different masses of extraction water and polyethylene glycol are added as extracting solutions, and the results show that the hesperidin and matrine contents of Examples 4 and 5 are greater than those of Examples 2 and 6, indicating that when the mass ratio of extraction water to polyethylene glycol is 20 to 16:1, the content of each Chinese medicine active ingredient in the obtained extract is higher.

[0035] Compared with Examples 5 and 7, Examples 8 to 11 simultaneously added the first polyethylene glycol and the second polyethylene glycol, and the results showed that the hesperidin and matrine contents of Examples 8 to 11 were greater than those of Examples 5 and 7, indicating that the first polyethylene glycol and the second polyethylene glycol played a synergistic role and could improve the extraction of effective components of each Chinese medicine. In Examples 8 to 11, the first polyethylene glycol and the second polyethylene glycol were added in different mass ratios, and the hesperidin and matrine contents of Examples 9 and 10 were greater than those of Examples 8 and 11, indicating that when the mass ratio of the first polyethylene glycol to the second polyethylene glycol was 2:2 to 3, the content of effective components in the obtained extract was higher.

[0036] Test 2 The veterinary Chinese medicine granules prepared in Example 10 were applied to a flock of chicks suffering from renal bronchitis.

[0037] A chicken farm in Yuanshi County, Shijiazhuang has 18,000 17-day-old chicks, which are cultivated in 2 sheds, with 9,000 chicks in each shed, and 10 to 20 chicks die every day in each shed. The two sheds are randomly divided into a control group and a test group. The control group has a normal diet, and the test group is fed with the Chinese medicine granules for veterinary use for preventing and treating poultry renal bronchitis obtained in embodiment 10 once a day, 0.3g for each chicken, 400kg of concentrated drinking water for each 1kg, and continuous medication for 5 days. During the test, the feed, drinking water and utensils used are strictly separated to avoid cross infection. The health status, drinking water, feeding and feces of the chicks are observed three times a day, and the morbidity and mortality of each group of chicks are recorded.

[0038] Symptoms of the disease: depression, decreased food intake, loose white watery stools, heaping, and chills; Incidence rate = total number of diseased chickens in each group / total number of test chickens in each group × 100%; Mortality rate = total number of dead chickens in each group / total number of experimental chickens in each group × 100%.

[0039] The test results are shown in Table 2 below: Table 2 Clinical observation record of 5-day use of veterinary Chinese medicine granules

[0040] As can be seen from Table 2, in the control group without drug intervention, the number of diseased chickens and the number of deaths were significantly larger. After the experimental group took the medicine, the morbidity and mortality of the chickens were effectively controlled, the feces morphology and condition of the chicks were significantly improved, and the feed intake increased, indicating that the veterinary Chinese medicine granules prepared in the experimental group can effectively achieve effective control of renal infectious bronchitis.

[0041] Test 3 The veterinary Chinese medicine granules prepared in Example 10 were applied to laying hens suffering from renal bronchitis.

[0042] A chicken farm in Gaocheng District, Shijiazhuang has 20,000 laying hens aged 200 days, which are raised in two sheds, with 10,000 laying hens in each shed. The two sheds are randomly divided into a control group and a test group. The control group has a normal diet, and the test group is fed with the Chinese medicine granules for preventing and treating poultry renal bronchitis prepared in Example 10 once a day, 0.25g per chicken, 450kg of concentrated drinking water per 1kg, and continuous medication for 5 days. During the test, the feed, drinking water and utensils used are strictly separated to avoid cross infection. The health status of the laying hens is observed, and the egg production rate and egg weight of each group are recorded.

[0043] The test results are shown in Table 3 below: Table 3 Clinical observation record of 5-day use of veterinary Chinese medicine granules

[0044] As shown in Table 3, in the control group that did not take the Chinese herbal granules, the morbidity and mortality rates of laying hens were high, the egg production rate and average egg weight were low, the egg quality was poor, and the chicken house environment was bad. After the experimental group took the Chinese herbal granules, the morbidity and mortality rates were effectively controlled, the egg production rate and average egg weight were significantly improved, the laying hens were well-proportioned, and the chicken house environment was good, indicating that the veterinary Chinese herbal granules show excellent efficacy in preventing and treating renal infectious bronchitis in laying hen farming.

[0045] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A Chinese medicinal granule for preventing and treating poultry renal bronchitis, characterized in that: The raw materials include the following components in parts by weight: 20-40 parts of patchouli, 40-50 parts of wormwood, 30-50 parts of isatis root, 40-60 parts of atractylodes, 50-70 parts of umbellatus, 40-60 parts of oriental rhizome, 30-40 parts of tangerine peel, 10-30 parts of sophora flavescens, 30-50 parts of platycodon, 50-70 parts of scutellaria baicalensis, 10-30 parts of polygala polyphylla, 30-50 parts of stir-fried yam, and 10-30 parts of roasted liquorice.

2. The veterinary Chinese medicine granules for preventing and treating poultry renal bronchitis according to claim 1, characterized in that: The raw materials include the following components in parts by weight: 30-35 parts of patchouli, 45-50 parts of wormwood, 35-45 parts of isatis root, 50-55 parts of atractylodes, 60-65 parts of umbellatus, 50-55 parts of oriental rhizome, 30-35 parts of tangerine peel, 20-25 parts of sophora flavescens, 35-45 parts of platycodon, 55-65 parts of scutellaria baicalensis, 15-25 parts of polygala japonica, 35-45 parts of stir-fried yam, and 20-30 parts of roasted liquorice.

3. The method for preparing a veterinary Chinese medicine granule for preventing and treating poultry renal bronchitis according to claim 1 or 2, characterized in that: The following steps are involved: S1. Weigh the above raw materials, add them into water, soak, decoct, filter to obtain a decoction, and then concentrate and filter to obtain an extract; S2, mixing the extract and auxiliary materials uniformly, granulating, drying, and granulating to obtain a granule semi-finished product, and packaging to obtain a veterinary Chinese medicine granule for preventing and treating poultry renal bronchitis; The auxiliary materials include powdered sugar and water-melted realgar.

4. The method for preparing a veterinary Chinese medicine granule for preventing and treating poultry renal bronchitis according to claim 3, characterized in that: Polyethylene glycol is also added during the soaking, and the mass ratio of the water to the polyethylene glycol is 20-16:

1.

5. The method for preparing a veterinary Chinese medicine granule for preventing and treating poultry renal bronchitis according to claim 4, characterized in that: The polyethylene glycol consists of a first polyethylene glycol and a second polyethylene glycol, and the molecular weight of the first polyethylene glycol is smaller than that of the second polyethylene glycol.

6. The method for preparing a veterinary Chinese medicine granule for preventing and treating poultry renal bronchitis according to claim 4, characterized in that: The molecular weight of the first polyethylene glycol is 270-330, the molecular weight of the second polyethylene glycol is 720-880, and the mass ratio of the first polyethylene glycol to the second polyethylene glycol is 2:2-3.

7. The method for preparing a veterinary Chinese medicine granule for preventing and treating poultry renal bronchitis according to claim 3, characterized in that: The decoction is performed twice, the first decoction time is 1.5-2.5 hours, and the second decoction time is 0.5-1.5 hours.

8. The method for preparing a veterinary Chinese medicine granule for preventing and treating poultry renal bronchitis according to claim 3, characterized in that: The concentration is carried out twice, the drug content of the first concentrated clear paste is 1.08-1.12 g / mL, and the drug content of the second concentrated extract is 1.28-1.32 g / mL.

9. The method for preparing a veterinary Chinese medicine granule for preventing and treating poultry renal bronchitis according to claim 3, characterized in that: The mass ratio of the extract, powdered sugar and water-melted realgar is 1:5:0.

01.

10. Use of the veterinary Chinese medicinal granules for preventing and treating poultry renal herpes simplex disease according to any one of claims 1 to 2 or the veterinary Chinese medicinal granules for preventing and treating poultry renal herpes simplex disease prepared by the preparation method according to any one of claims 3 to 9 in the prevention and treatment of poultry renal herpes simplex disease.

Images