Integrated nucleic acid detection device compatible with multiple sample types and nucleic acid detection method

Abstract

The invention relates to the technical field of nucleic acid detection, and discloses an integrated nucleic acid detection device compatible with multiple sample types and a nucleic acid detection method.The nucleic acid detection device comprises multiple cavities, and a saliva sample bin, a plasma sample bin and a nasopharynx swab sample bin are arranged at the top end of a nucleic acid extraction cavity; the test strip detection chamber comprises a first test paper chamber and a second test paper chamber, and the second test paper chamber is in sliding connection with the first test paper chamber; a through hole penetrating through the two end faces of each cavity is formed in each cavity, thorn-shaped structures extending towards the top faces of the cavities are arranged on the side walls of the through holes, the bottom ends of the through holes are sealed through sealing films, and the thorn-shaped structures are used for puncturing the corresponding sealing films; the test paper is fixed in the test paper storage groove of the second test paper chamber, the test paper storage groove is communicated with the through hole in the first test paper chamber, and the test paper strip detection chamber is provided with a detection result output window for outputting a detection result. Detection is easy to operate, the detection process is in a sealed state, environmental pollution is avoided, and various samples can be compatible.

Description

Technical Field

[0001] The present invention relates to the technical field of nucleic acid detection, and in particular to an integrated nucleic acid detection device and a nucleic acid detection method that are compatible with multiple sample types. Background Art

[0002] Pathogenic microorganisms are common hazards, and rapid and accurate diagnostic methods are the primary condition for effectively reducing the spread of pathogens. Nucleic acid testing includes the steps of preliminary sampling, nucleic acid extraction, nucleic acid amplification and nucleic acid testing. This process is very likely to cause environmental pollution, so professionals, professional equipment and specific locations (such as hospitals, laboratories, etc.) are required, which greatly increases the cost and time of testing.

[0003] In addition, portable nucleic acid detection devices have shown great potential in the field of point-of-care (POC) due to their compact design and convenient operation. However, existing portable nucleic acid detection devices are difficult to effectively accommodate multiple sample types that require different pretreatment steps, such as saliva, nasopharyngeal swabs, and plasma samples. These sample types have significant differences in composition, resulting in different pretreatment requirements.

[0004] Saliva samples usually contain higher concentrations of enzymes and proteins, which may inhibit the subsequent nucleic acid amplification process; nasopharyngeal swabs need to undergo a specific lysis step to release nucleic acids in pathogens; plasma samples need to remove blood cells and other components that may interfere with the test results. These differentiated pre-processing steps are relatively easy to implement in traditional laboratory environments, but they are particularly difficult in portable nucleic acid testing devices due to their small size and technical complexity.

[0005] In view of this, there is an urgent need to develop a new type of portable nucleic acid detection device that is effectively compatible with various sample types and can integrate necessary sample preprocessing functions in a limited space. Summary of the invention

[0006] Therefore, the technical problem to be solved by the present invention is that the existing portable nucleic acid detection devices are not compatible with the detection of saliva, nasopharyngeal swabs and plasma samples at the same time, so as to provide an integrated nucleic acid detection device and nucleic acid detection method that are compatible with multiple sample types.

[0007] In order to solve the above technical problems, the technical solution of the present invention is as follows:

[0008] An integrated nucleic acid detection device compatible with multiple sample types, the nucleic acid detection device comprises a nucleic acid extraction chamber, a first buffer chamber for accommodating a buffer solution, a nucleic acid amplification chamber, a second buffer chamber for accommodating a diluent, and a test strip detection chamber, which are threaded and sealed in sequence; wherein:

[0009] A sample compartment is provided at the top of the nucleic acid extraction chamber, and the sample compartment includes a saliva sample compartment, a plasma sample compartment, and a nasopharyngeal swab sample compartment, each of which has an injection port on the top. The injection ports of the saliva sample compartment, the plasma sample compartment, and the nasopharyngeal swab sample compartment are detachably sealed by a saliva compartment sealing plug, a plasma compartment sealing plug, and a nasopharyngeal swab compartment sealing plug, respectively. The bottom ends of the saliva sample compartment and the plasma sample compartment are sealed by a saliva compartment sealing membrane and a plasma compartment filter membrane, respectively.

[0010] The test paper detection chamber comprises a first test paper chamber and a second test paper chamber, and the second test paper chamber is slidably connected to the first test paper chamber along the length direction of the nucleic acid detection device;

[0011] Each of the chambers is provided with a through hole penetrating through both end surfaces, and a thorn-like structure extending toward the top surface is provided on the side wall of the through hole, the bottom end of the through hole is sealed by a sealing film, and the thorn-like structure is used to pierce the corresponding sealing film;

[0012] The test paper is fixed in the test paper storage slot of the second test paper chamber, the second test paper storage slot is connected to the through hole in the first test paper chamber, and the test paper strip detection chamber is provided with a test result output window for outputting the test result.

[0013] Furthermore, the through hole comprises a connected internal thread section and a light hole section, the diameter of the internal thread section is larger than the diameter of the light hole section, so as to form a step surface at the transition between the internal thread section and the light hole section, and the thorn-like structure is protruding from the step surface.

[0014] Furthermore, a plurality of the thorn-like structures are provided on the step surface.

[0015] Furthermore, the light hole section includes a conical cavity and a flow-blocking channel, the top end of the flow-blocking channel is connected to the conical cavity, and the bottom end is connected to the second buffer chamber when the corresponding sealing film is broken.

[0016] Furthermore, the optical hole section of the nucleic acid amplification chamber also includes a storage chamber, the sealing film seals the bottom end of the storage chamber, the top end of the storage chamber is connected to the flow blocking channel, and the bottom end is connected to the second buffer chamber when the corresponding sealing film is broken.

[0017] Furthermore, the cross-section of the flow-blocking channel is circular, and the diameter is 0.5 mm to 1 mm.

[0018] Furthermore, the nucleic acid extraction chamber, the first buffer chamber, the nucleic acid amplification chamber, and the second buffer chamber are collectively referred to as chambers, and each chamber has an external thread section on the outer wall of the bottom end, and the length of the external thread section is equivalent to the length of the corresponding internal thread section.

[0019] Furthermore, the second test paper chamber comprises two sub-bodies which are relatively buckled together, and grooves are respectively provided on the opposite surfaces of the two sub-bodies, and the two grooves are matched to form the test paper storage slot, and the opposite surfaces are the surfaces of the sub-bodies facing each other.

[0020] Furthermore, a receiving groove for receiving the bottom end of the first test paper chamber is provided at the top of the second test paper chamber, the top of the test paper storage groove is connected to the receiving groove, a slider / slide groove is provided on the side wall of the receiving groove, and a slide groove / slider is correspondingly provided on the outer side wall of the first test paper chamber, and the slider slides in cooperation with the slide groove.

[0021] The technical solution of the present invention has the following advantages:

[0022] 1. The integrated nucleic acid detection device provided by the present invention is compatible with multiple sample types. The nucleic acid extraction chamber, the first buffer chamber, the nucleic acid amplification chamber, the second buffer chamber and the first test paper chamber are collectively referred to as chambers. The two adjacent chambers are connected by a threaded structure. The liquid is transferred by rotating the chamber to pierce the corresponding sealing film, and the nucleic acid extraction, amplification, detection and other processes can be completed in sequence. The operation is simple, and does not require reliance on professionals and professional equipment, which reduces the detection cost and time. In addition, a saliva sample chamber, a plasma sample chamber, and a nasopharyngeal swab sample chamber are provided on the top of the nucleic acid extraction chamber for saliva, plasma, and nasopharyngeal swab samples, respectively, so that the nucleic acid detection device can be compatible with multiple sample types, reduces dependence on specific sample collection technology, increases the flexibility of detection, and improves the efficiency of large-scale screening and monitoring. In addition, the device is in a completely sealed state during the entire detection process to avoid environmental pollution.

[0023] 2. The integrated nucleic acid detection device compatible with a variety of sample types provided by the present invention, the light hole section of the nucleic acid amplification chamber includes a conical cavity and a flow-blocking channel, the top of the flow-blocking channel is connected to the conical cavity, and the bottom is connected to the second buffer chamber when the corresponding sealing film is broken. The setting of the conical cavity is conducive to the smooth flow of the amplification product into the second buffer chamber. When the nucleic acid detection device is inverted, the flow-blocking channel can prevent the liquid from flowing from the second buffer chamber via the nucleic acid amplification chamber into the first buffer chamber, so that the loading end of the test paper can contact the detection liquid.

[0024] 3. The present invention provides an integrated nucleic acid detection device that is compatible with multiple sample types. The nucleic acid extraction chamber, the first buffer chamber, the nucleic acid amplification chamber, the second buffer chamber and the first test paper chamber are collectively referred to as chambers. Each chamber has an external thread section on the outer wall at the bottom end. The length of the external thread section is equivalent to the length of the internal thread section in the adjacent chamber below. In this way, even if the size of the thorn-like structure is small, it can pierce the sealing film without designing the size of the thorn-like structure to be larger, thereby increasing the processing cost and difficulty.

[0025] A nucleic acid detection method based on the aforementioned integrated nucleic acid detection device compatible with multiple sample types comprises the following steps:

[0026] Add the sample into the corresponding sample chamber through the corresponding injection port, seal the corresponding injection port, and allow the sample to enter the nucleic acid extraction chamber along the sample chamber, and obtain a cleavage product after the reaction;

[0027] Twisting the nucleic acid extraction chamber toward the first buffer chamber to puncture the sealing film at the bottom of the nucleic acid extraction chamber, so that the lysate enters the first buffer chamber and mixes with the buffer solution;

[0028] Twisting the first buffer chamber toward the nucleic acid amplification chamber to puncture the sealing film at the bottom of the first buffer chamber, so that the cleavage product and the buffer solution enter the nucleic acid amplification chamber, and obtaining an amplification product after the reaction;

[0029] Twisting the nucleic acid amplification chamber toward the second buffer chamber, puncturing the sealing film at the bottom of the nucleic acid amplification chamber, and shaking the nucleic acid detection device so that the amplification product enters the second buffer chamber and is diluted to obtain a detection solution;

[0030] Inverted nucleic acid detection device;

[0031] Twisting the first test paper chamber toward the second buffer chamber to puncture the sealing film at the bottom of the second buffer chamber;

[0032] Pushing the second test paper chamber toward the first test paper chamber so that the sample loading end of the test paper contacts the detection liquid;

[0033] After the preset time has passed, the test results will be output from the test result output window.

[0034] The technical solution of the present invention has the following advantages:

[0035] The present invention provides a nucleic acid detection method for an integrated nucleic acid detection device that is compatible with multiple sample types. Liquid transfer can be performed by rotating the chamber to puncture the corresponding sealing film to complete nucleic acid extraction, amplification, detection and other processes in sequence. The operation is simple, does not require reliance on professionals or professional equipment, reduces detection costs and reduces detection time. In addition, the device is in a completely sealed state during the entire detection process to avoid environmental pollution. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] In order to more clearly illustrate the specific implementation methods of the present invention or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some implementation methods of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0037] Figure 1 It is a three-dimensional perspective view of a nucleic acid detection device in an embodiment of the present invention;

[0038] Figure 2 is a three-dimensional perspective view of a sample bin in an embodiment of the present invention;

[0039] Figure 3 is a three-dimensional schematic diagram of a saliva bin sealing plug in an embodiment of the present invention;

[0040] Figure 4 is a three-dimensional schematic diagram of a first buffer chamber in an embodiment of the present invention;

[0041] Figure 5 is a front view schematic diagram of a nucleic acid amplification chamber in an embodiment of the present invention;

[0042] Figure 6 is a cross-sectional schematic diagram of a nucleic acid amplification chamber in an embodiment of the present invention;

[0043] Figure 7 This is a three-dimensional schematic diagram of the first test paper chamber at a first angle in an embodiment of the present invention;

[0044] Figure 8 This is a three-dimensional schematic diagram of the first test paper chamber at a second angle in an embodiment of the present invention;

[0045] Fig. 9 is a three-dimensional exploded schematic diagram of the second test paper chamber in an embodiment of the present invention;

[0046] Fig.10 Flow chart of nucleic acid detection in an embodiment of the present invention.

[0047] 11. Saliva sample chamber; 110. Saliva chamber sealing film; 111. Saliva chamber sealing plug; 112. Diversion groove; 12. Plasma sample chamber; 120. Plasma chamber filter membrane; 121. Plasma chamber sealing plug; 13. Nasopharyngeal swab sample chamber; 131. Nasopharyngeal swab chamber sealing plug; 2. Nucleic acid extraction chamber; 3. First buffer chamber; 4. Nucleic acid amplification chamber; 41. Conical chamber; 42. Blocking channel; 43. Storage chamber ; 5. Second buffer chamber; 6. Test strip detection chamber; 61. First test strip chamber; 6111. Slide groove; 62. Second test strip chamber; 621. Receiving groove; 6211. Sliding block; 622. Test strip storage groove; 623. Test result output window; 624. Buckling structure; A. Through hole; A1. Internal thread section; A2. Light hole section; A3. Step surface; B. Thorn-like structure; C. Sealing film; D. External thread section. DETAILED DESCRIPTION

[0048] The technical solution of the present invention will be described clearly and completely below in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0049] In the description of the present invention, it should be noted that the directions or positional relationships indicated by the terms "upper", "lower", "left", "right", "inner", "outer", etc. are based on the directions or positional relationships shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore cannot be understood as limiting the present invention. In addition, the terms "first" and "second" are only used for descriptive purposes and cannot be understood as indicating or implying relative importance.

[0050] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0051] In addition, the technical features involved in the different embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.

[0052] Example 1

[0053] like Figures 1 to 9As shown, this embodiment provides an integrated nucleic acid detection device (hereinafter referred to as nucleic acid detection device) that is compatible with multiple sample types. The nucleic acid detection device includes a nucleic acid extraction chamber 2, a first buffer chamber 3 for accommodating a buffer solution, a nucleic acid amplification chamber 4, a second buffer chamber 5 for accommodating a diluent, and a test strip detection chamber 6, which are threaded and sealed together in sequence. A sample compartment is provided on the top of the nucleic acid extraction chamber 2, as shown in FIG. Figure 2 As shown, the sample compartment includes a saliva sample compartment 11, a plasma sample compartment 12, and a nasopharyngeal swab sample compartment 13, each of which has an injection port on the top. The injection ports of the saliva sample compartment 11, the plasma sample compartment 12, and the nasopharyngeal swab sample compartment 13 are detachably sealed by a saliva compartment sealing plug 111, a plasma compartment sealing plug 121, and a nasopharyngeal swab compartment sealing plug 131, respectively. The bottom ends of the saliva sample compartment 11 and the plasma sample compartment 12 are sealed by a saliva compartment sealing membrane 110 and a plasma compartment filter membrane 120, respectively. The test strip detection chamber 6 includes a first test paper chamber 61 and a second test paper chamber 62, and the second test paper chamber 62 is slidably connected to the first test paper chamber 61 along the length direction of the nucleic acid detection device. The nucleic acid extraction chamber 2, the first buffer chamber 3, the nucleic acid amplification chamber 4, the second buffer chamber 5 and the first test paper chamber 61 are all provided with through holes A penetrating through the two end faces thereof, and the side walls of the through holes A are provided with thorn-like structures B extending toward the respective top faces, the bottom ends of the through holes A are sealed by sealing films C, and the thorn-like structures B are used to pierce the corresponding sealing films C. The test paper is fixed in the test paper storage slot 622 of the second test paper chamber 62. The test paper storage slot 622 is connected to the through hole A in the first test paper chamber 61. The test paper strip detection chamber 6 is provided with a test result output window 623 for outputting the test result.

[0054] The present embodiment provides an integrated nucleic acid detection device that is compatible with multiple sample types, including a nucleic acid extraction chamber 2, a first buffer chamber 3, a nucleic acid amplification chamber 4, a second buffer chamber 5, and a test strip detection chamber 6, which are threadedly sealed and connected in sequence. The corresponding sealing films are pierced by rotating the chambers to transfer liquids, so as to complete nucleic acid extraction, amplification, detection and other processes in sequence without relying on professionals and professional equipment. The operation is convenient and instant detection can be achieved. In addition, since the nucleic acid detection device is provided with a saliva sample chamber 11, a plasma sample chamber 12, and a nasopharyngeal swab sample chamber 13 on the top of the nucleic acid extraction chamber 2, it can effectively be compatible with various sample types and can integrate necessary sample pretreatment functions in a limited space. Moreover, the device is in a completely sealed state during the entire detection process to avoid environmental pollution.

[0055] The number and order of the aforementioned chambers can be increased, deleted or exchanged according to experimental methods and requirements.

[0056] In this embodiment, since the nucleic acid extraction chamber 2, the first buffer chamber 3, the nucleic acid amplification chamber 4, the second buffer chamber 5, and the test strip detection chamber 6 are threadedly sealed, any chamber can be replaced as needed (for example, the contents of a chamber need to be replaced due to expiration or the experimental method needs to be changed) to avoid discarding the entire acid detection device and causing a waste of resources.

[0057] Furthermore, if Figure 4 As shown, taking the through hole A in the first buffer chamber 3 as an example, the structure of the through hole A is introduced. The structures of the through hole A in the nucleic acid extraction chamber 2, the nucleic acid amplification chamber 4, the second buffer chamber 5 and the first test paper chamber 61 are basically the same and will not be repeated here. The through hole A includes an internal thread segment A1 and a light hole segment A2 that are interconnected. The diameter of the internal thread segment A1 is larger than the diameter of the light hole segment A2, so as to form a step surface A3 at the junction of the internal thread segment A1 and the light hole segment A2. The thorn-like structure B protrudes from the step surface A3. Specifically, the cross-section of the through hole A is circular. A plurality of thorn-like structures B are provided at equal intervals on each step surface A3.

[0058] Furthermore, if Figure 4 As shown, the optical hole section A2 of the nucleic acid amplification chamber 4 includes a conical cavity 41 and a blocking channel 42. The top of the blocking channel 42 is connected to the conical cavity 41, and the bottom is connected to the second buffer chamber 5 when the corresponding sealing film C is broken. Specifically, the cross section of the blocking channel 42 is circular, and the diameter is 0.5 mm to 1 mm.

[0059] Furthermore, the optical hole section A2 of the nucleic acid amplification chamber 4 also includes a storage chamber 43, the sealing film C seals the bottom end of the storage chamber 43, the top end of the storage chamber 43 is connected to the flow blocking channel 42, and the bottom end is connected to the second buffer chamber 5 when the corresponding sealing film C is broken.

[0060] Further, the nucleic acid extraction chamber 2, the first buffer chamber 3, the nucleic acid amplification chamber 4, and the second buffer chamber 5 are collectively referred to as chambers. The nucleic acid extraction chamber 2, the first buffer chamber 3, the nucleic acid amplification chamber 4, and the second buffer chamber 5 all have external thread segments D on their bottom outer walls, and the length of the external thread segments D is equivalent to the length of the corresponding internal thread segments A1, so that even if the length of the thorn-like structure B is small, it can pierce the sealing film C without designing the size of the thorn-like structure B to be longer, thereby increasing the processing cost and difficulty.

[0061] Furthermore, the second test paper chamber 62 includes two sub-bodies that are relatively buckled together, and grooves are respectively provided on the opposite surfaces of the two sub-bodies. The two grooves are matched to form a test paper storage slot 622, and the opposite surfaces are the surfaces of the sub-bodies facing each other. A buckling structure 624 is correspondingly provided on the opposite surfaces. In this embodiment, Fig. 9From the perspective of the embodiment, the buckling structure 624 includes a groove arranged on the left sub-body and a corresponding clamping column arranged on the right sub-body, and the clamping column cooperates with the groove to fix the two sub-bodies together.

[0062] Furthermore, a receiving groove 621 for receiving the bottom end of the first test paper chamber 61 is provided at the top of the second test paper chamber 62, and the top of the test paper storage groove 622 is connected to the receiving groove 621, and a slider 6211 / slide groove is provided on the side wall of the receiving groove 621, and a slide groove 6111 / slide groove is correspondingly provided on the outer side wall of the first test paper chamber 61, and the slider 6211 slides in cooperation with the slide groove 6111.

[0063] Furthermore, the nucleic acid detection device is cylindrical or rectangular in shape as a whole.

[0064] Example 2

[0065] like Fig.10 As shown, the following takes saliva samples as an example to introduce an integrated nucleic acid detection device (such as Figures 1 to 9 The nucleic acid detection method of the present invention comprises the following steps:

[0066] Place the nucleic acid detection device upright, with the top facing up and the bottom facing down;

[0067] Remove the saliva chamber sealing plug 111 to expose the sampling port of the saliva sample chamber 11;

[0068] Add saliva into the saliva sample chamber 11 from the sampling port, cover the saliva chamber sealing plug 111, and press down to the bottom to puncture the saliva chamber sealing film 110, so that the pre-treated saliva sample passes through the guide groove 112 (such as Figure 3 As shown) enters the nucleic acid extraction chamber 2, and obtains a cleavage product after a preset reaction time;

[0069] Twist the nucleic acid extraction chamber 2 toward the first buffer chamber 3 to pierce the sealing film C at the bottom of the nucleic acid extraction chamber 2, so that the lysate enters the first buffer chamber 3 and mixes with the buffer solution;

[0070] The first buffer chamber 3 is twisted toward the nucleic acid amplification chamber 4 to puncture the sealing film C at the bottom of the first buffer chamber 3 so that the cleavage product and the buffer solution enter the nucleic acid amplification chamber 4, and the amplification product is obtained after a predetermined reaction time;

[0071] Twist the nucleic acid amplification chamber 4 toward the second buffer chamber 5 to puncture the sealing film C at the bottom of the nucleic acid amplification chamber 4, and swing the nucleic acid detection device to allow the amplification product to enter the second buffer chamber 5 and be diluted to obtain the detection solution;

[0072] Inverted nucleic acid detection device;

[0073] The first test paper chamber 61 is twisted toward the second buffer chamber 5 to pierce the sealing film C at the bottom of the second buffer chamber 5. The diluted amplification product in the second buffer chamber 5 flows toward the nucleic acid amplification chamber 4. However, due to the blocking effect of the blocking channel 42, the test liquid does not enter the first buffer chamber 3 through the blocking channel 42, and part of it enters the aforementioned storage chamber 43.

[0074] Push the second test paper chamber 62 toward the first test paper chamber 61, so that the slider 6211 slides along the slide groove 6111, and the sample loading end of the test paper moves along with the second test paper chamber 62, enters the second buffer chamber 5, and contacts the detection liquid;

[0075] After being left for a preset time, the detection result is outputted through the detection result output window 623 .

[0076] The detection process of plasma samples and nasopharyngeal swab samples is the same as that of saliva samples, except that the plasma samples need to be added to the plasma sample chamber 12 and the nasopharyngeal swab samples need to be added to the nasopharyngeal swab sample chamber 13 .

[0077] The present invention provides a nucleic acid detection method for an integrated nucleic acid detection device that is compatible with multiple sample types. The method can complete nucleic acid extraction, amplification, detection and other processes in sequence by rotating the chamber to puncture the corresponding sealing film to transfer liquid. The operation is simple, does not require reliance on professionals or professional equipment, reduces detection costs and reduces detection time. In addition, the device is in a completely sealed state during the entire detection process to avoid environmental pollution.

[0078] Obviously, the above embodiments are merely examples for the purpose of clear explanation, and are not intended to limit the implementation methods. For those skilled in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the implementation methods here. The obvious changes or modifications derived therefrom are still within the scope of protection of the invention.

Claims

1. An integrated nucleic acid detection device compatible with multiple sample types, characterized in that: The nucleic acid detection device comprises a nucleic acid extraction chamber (2), a first buffer chamber (3) for accommodating a buffer solution, a nucleic acid amplification chamber (4), a second buffer chamber (5) for accommodating a diluent, and a test strip detection chamber (6) which are threadedly and sealedly connected in sequence; wherein: A sample chamber is provided at the top of the nucleic acid extraction chamber (2), and the sample chamber comprises a saliva sample chamber (11), a plasma sample chamber (12), and a nasopharyngeal swab sample chamber (13), each of which has an injection port at the top; the injection ports of the saliva sample chamber (11), the plasma sample chamber (12), and the nasopharyngeal swab sample chamber (13) are respectively detachably sealed by a saliva chamber sealing plug (111), a plasma chamber sealing plug (121), and a nasopharyngeal swab chamber sealing plug (131); and the bottom ends of the saliva sample chamber (11) and the plasma sample chamber (12) are respectively sealed by a saliva chamber sealing membrane (110) and a plasma chamber filtering membrane (120); The test paper detection chamber (6) comprises a first test paper chamber (61) and a second test paper chamber (62), wherein the second test paper chamber (62) is slidably connected to the first test paper chamber (61) along the length direction of the nucleic acid detection device; The nucleic acid extraction chamber (2), the first buffer chamber (3), the nucleic acid amplification chamber (4), the second buffer chamber (5) and the first test paper chamber (61) are all provided with through holes (A) penetrating through the respective two end surfaces, and thorn-like structures (B) extending toward the respective top surfaces are provided on the side walls of the through holes (A), the bottom ends of the through holes (A) are sealed by sealing films (C), and the thorn-like structures (B) are used to pierce the corresponding sealing films (C); The test paper is fixed in the test paper storage slot (622) of the second test paper chamber (62); the test paper storage slot (622) is connected to the through hole (A) in the first test paper chamber (61); and the second test paper chamber (62) is provided with a test result output window (623) for outputting the test result.

2. The integrated nucleic acid detection device compatible with multiple sample types according to claim 1, characterized in that: The through hole (A) comprises an internal thread section (A1) and a light hole section (A2) which are connected to each other. The diameter of the internal thread section (A1) is larger than the diameter of the light hole section (A2), so as to form a step surface (A3) at the transition point between the internal thread section (A1) and the light hole section (A2), and the thorn-like structure (B) is protrudingly arranged on the step surface (A3).

3. The integrated nucleic acid detection device compatible with multiple sample types according to claim 2, characterized in that: A plurality of thorn-like structures (B) are provided on the step surface (A3).

4. The integrated nucleic acid detection device compatible with multiple sample types according to claim 2, characterized in that: The light hole section (A2) of the nucleic acid amplification chamber (4) comprises a conical cavity (41) and a flow-blocking channel (42); the top end of the flow-blocking channel (42) is connected to the conical cavity (41), and the bottom end is connected to the second buffer chamber (5) when the corresponding sealing film (C) is broken.

5. The integrated nucleic acid detection device compatible with multiple sample types according to claim 4, characterized in that: The optical hole section (A2) of the nucleic acid amplification chamber (4) further comprises a storage chamber (43), the sealing film (C) seals the bottom end of the storage chamber (43), the top end of the storage chamber (43) is connected to the flow blocking channel (42), and the bottom end is connected to the second buffer chamber (5) when the corresponding sealing film (C) is broken.

6. The integrated nucleic acid detection device compatible with multiple sample types according to claim 4, characterized in that: The cross section of the flow blocking channel (42) is circular, and the diameter is 0.5 mm to 1 mm.

7. The integrated nucleic acid detection device compatible with multiple sample types according to claim 2, characterized in that: The nucleic acid extraction chamber (2), the first buffer chamber (3), the nucleic acid amplification chamber (4), and the second buffer chamber (5) are collectively referred to as chambers, and each chamber has an external thread section (D) on its bottom outer wall, and the length of the external thread section (D) is equivalent to the length of the corresponding internal thread section (A1).

8. The integrated nucleic acid detection device compatible with multiple sample types according to any one of claims 1 to 7, characterized in that: The second test paper chamber (62) comprises two sub-bodies which are relatively buckled together, and grooves are respectively provided on the opposite surfaces of the two sub-bodies. The two grooves are matched to form the test paper storage slot (622), and the opposite surfaces are the surfaces on the sub-bodies facing each other.

9. The integrated nucleic acid detection device compatible with multiple sample types according to any one of claims 1 to 7, characterized in that: The top of the second test paper chamber (62) is provided with a receiving groove (621) for receiving the bottom end of the first test paper chamber (61); the top of the test paper storage groove (622) is connected to the receiving groove (621); a slider (6211) / slide groove is provided on the side wall of the receiving groove (621); a slide groove (6111) / slide groove is correspondingly provided on the outer side wall of the first test paper chamber (61); the slider (6211) is slidably matched with the slide groove (6111).

10. A nucleic acid detection method based on the integrated nucleic acid detection device compatible with multiple sample types according to any one of claims 1 to 9, characterized in that: The steps include: Adding the sample into the sample chamber through the corresponding injection port, sealing the corresponding injection port, allowing the sample to enter the nucleic acid extraction chamber (2) along the sample chamber, and obtaining a cleavage product after the reaction; Twisting the nucleic acid extraction chamber (2) toward the first buffer chamber (3) to puncture the sealing film (C) at the bottom of the nucleic acid extraction chamber (2) so that the lysate enters the first buffer chamber (3) and mixes with the buffer solution; Twisting the first buffer chamber (3) toward the nucleic acid amplification chamber (4) to puncture the sealing film (C) at the bottom of the first buffer chamber (3) so that the cleavage product and the buffer solution enter the nucleic acid amplification chamber (4) and obtain an amplification product after the reaction; Twisting the nucleic acid amplification chamber (4) toward the second buffer chamber (5), puncturing the sealing film (C) at the bottom of the nucleic acid amplification chamber (4), and shaking the nucleic acid detection device so that the amplification product enters the second buffer chamber (5) and is diluted to obtain a detection solution; Inverting the nucleic acid detection device; Twisting the first test paper chamber (61) toward the second buffer chamber (5) to puncture the sealing film (C) at the bottom of the second buffer chamber (5); Pushing the second test paper chamber (62) toward the first test paper chamber (61) so that the sample loading end of the test paper contacts the detection liquid; After being left to stand for a preset time, the detection result is outputted from the detection result output window (623).

Images