Integrated nucleic acid detection device based on flexible structure and push-pull structure

Abstract

The invention relates to the technical field of nucleic acid detection, and discloses a nucleic acid integrated detection device based on a flexible structure and a push-pull structure, which comprises a detection main body, an upper cover, a bottom shell and an extrusion block arranged between the upper cover and the bottom shell, the detection main body is arranged in an accommodating cavity between the upper cover and the bottom shell; the detection main body comprises a sample introduction tube, a nucleic acid extraction-free reagent chamber, a first buffer solution chamber, a nucleic acid amplification reagent chamber, a second buffer solution chamber, a uniform mixing chamber, a sample loading groove and chromatography test paper which are connected in sequence, wherein the nucleic acid extraction-free reagent chamber, the first buffer solution chamber, the nucleic acid amplification reagent chamber, the second buffer solution chamber and the uniform mixing chamber are flexible reagent chambers; the head end of the sample loading groove is connected with the uniform mixing chamber, and the tail end of the sample loading groove is connected with the chromatography test paper; and the extrusion block can move from one end of the upper cover to the other end and is used for extruding the liquid in the detection main body and forcing the liquid to flow downstream. The liquid is extruded to flow downstream only when the extrusion block is manually pushed to move, so that the controllability of the detection process can be realized.

Description

Technical Field

[0001] The present invention relates to the technical field of nucleic acid detection, and in particular to an integrated nucleic acid detection device based on a flexible structure and a push-pull structure. Background Art

[0002] Molecular diagnostic technology plays a very important role in pathogen detection, among which nucleic acid detection is the most reliable and direct method. Conventional nucleic acid detection technology mainly refers to fluorescent quantitative PCR, but this process must rely on large-scale instruments and equipment, as well as laboratory personnel with professional knowledge reserves, which limits its application in instant nucleic acid detection.

[0003] In addition, in recent years, the expansion of testing needs and the enrichment of testing scenarios have promoted the rapid development of instant nucleic acid testing. Once the existing instant nucleic acid testing equipment starts testing, it will continue until the test results are displayed. However, the test personnel will inevitably be interrupted during the test process, and when they return to the test site, the test results may no longer be accurate due to the long time interval.

[0004] Therefore, it is necessary to provide a new detection device to overcome the above-mentioned defects. Summary of the invention

[0005] The object of the present invention is to provide an integrated nucleic acid detection device based on a flexible structure and a push-pull structure with a controllable detection process.

[0006] In order to solve the above technical problems, the technical solution of the present invention is as follows:

[0007] A nucleic acid integrated detection device based on a flexible structure and a push-pull structure comprises a detection body, an upper cover, a bottom shell, and an extrusion block installed between the upper cover and the bottom shell;

[0008] The detection body is arranged in the accommodating chamber between the upper cover and the bottom shell; the detection body comprises an injection tube, a nucleic acid-free extraction reagent chamber, a first buffer chamber, a nucleic acid amplification reagent chamber, a second buffer chamber, a mixing chamber, a sample loading groove and a chromatography test paper which are connected in sequence; the nucleic acid-free extraction reagent chamber, the first buffer chamber, the nucleic acid amplification reagent chamber, the second buffer chamber and the mixing chamber are all flexible reagent chambers; the head end of the sample loading groove is connected to the mixing chamber, and the tail end of the sample loading groove is connected to the chromatography test paper; wherein;

[0009] The squeezing block can move from one end of the upper cover to the other end, and is used for squeezing the liquid in the detection body, forcing the liquid to flow downstream.

[0010] Furthermore, two adjacent flexible reagent chambers are divided into two independent chambers by a weld, and one third of the length of the weld is a cold weld.

[0011] Furthermore, an upper slide groove is provided on the upper cover, a partition wall is provided inside the bottom shell, and the extrusion block includes a handle, a neck and an extrusion head connected in sequence, the handle protrudes from the top surface of the upper cover, the neck slides along the upper slide groove, and the extrusion head is located between the partition wall and the long side wall of the bottom shell.

[0012] Furthermore, a side slide groove is inwardly recessed on a side of the partition wall facing the extrusion head, and the extrusion head slides along the side slide groove.

[0013] Furthermore, a locking structure is recessed on the side wall of the upper slide groove corresponding to the first buffer chamber, and a limiting protrusion is provided on the neck, and the limiting protrusion cooperates with the locking structure.

[0014] Furthermore, the detection device also includes a U-shaped buckle, the opening of the buckle faces the neck and is clamped on the neck, and the bottom surface of the buckle abuts against the top surface of the upper cover, so that the slider does not squeeze the liquid in the detection body.

[0015] Furthermore, the sample loading groove is arranged parallel to the short side wall of the bottom shell, the chromatography test paper is arranged parallel to the long side wall of the bottom shell, and a test result display window is opened on the upper cover corresponding to the chromatography test paper.

[0016] Further, the head end of the sample injection tube is clamped on a short side wall of the bottom shell and protrudes from the short side wall, or the head end of the sample injection tube is clamped on the top surface of the upper cover and protrudes from the top surface of the upper cover.

[0017] Furthermore, the sample injection tube is connected to the nucleic acid-free extraction reagent chamber via a rigid pipe and a flexible pipe in sequence, and the rigid pipe is arranged to be inclined toward the bottom wall of the bottom shell.

[0018] Furthermore, it also includes two heating modules, which are used to heat the nucleic acid-free extraction reagent chamber and the nucleic acid amplification reagent chamber respectively.

[0019] The technical solution of the present invention has the following advantages:

[0020] 1. The nucleic acid integrated detection device based on flexible structure and push-pull structure provided by the present invention comprises a detection body, an upper cover, a bottom shell, and an extrusion block installed between the upper cover and the bottom shell; the detection body is arranged in the accommodating cavity between the upper cover and the bottom shell; the detection body comprises an injection tube, a nucleic acid-free extraction reagent chamber, a first buffer chamber, a nucleic acid amplification reagent chamber, a second buffer chamber, a mixing chamber, a sample loading groove and a chromatography test paper connected in sequence; the nucleic acid-free extraction reagent chamber, the first buffer chamber, the nucleic acid amplification reagent chamber, the second buffer chamber, and the mixing chamber are all flexible reagent chambers; the head end of the sample loading groove is connected to the mixing chamber, and the tail end of the sample loading groove is connected to the chromatography test paper; wherein the extrusion block moves from one end of the upper cover to the other end, and is used to squeeze the liquid in the detection body and force the liquid to flow downstream, so that the liquid will be squeezed to flow downstream only when the extrusion block is manually pushed to move, thereby realizing the controllability of the detection process.

[0021] 2. The integrated nucleic acid detection device based on flexible structure and push-pull structure provided by the present invention has two adjacent flexible reagent chambers divided into two independent chambers by a weld, and one-third of the length of the weld is a virtual weld. In this way, the independence of the two adjacent flexible reagent chambers can be guaranteed, and when the extrusion block squeezes the flexible reagent chamber, the two adjacent flexible reagent chambers can be smoothly connected, thereby ensuring the smooth progress of the detection.

[0022] 3. The integrated nucleic acid detection device based on a flexible structure and a push-pull structure provided by the present invention has a recessed locking structure on the side wall of the upper slide groove corresponding to the first buffer chamber, and a limiting protrusion is provided on the neck of the extrusion block. The limiting protrusion cooperates with the locking structure to indicate that the extrusion block has slid into place, thereby avoiding excessive movement of the extrusion block and further improving the controllability of the detection process.

[0023] 4. The present invention provides an integrated nucleic acid detection device based on a flexible structure and a push-pull structure, and the detection device also includes a U-shaped buckle, the opening of the buckle faces the neck and is clamped on the neck, the bottom surface of the buckle is against the top surface of the upper cover, so that the slider will not produce an squeezing effect on the liquid in the detection body. The setting of the buckle can prevent the squeezing block from unexpectedly squeezing the detection body, and prevent the detection body from being scrapped due to accidental squeezing of the squeezing block.

[0024] 5. In the integrated nucleic acid detection device based on a flexible structure and a push-pull structure provided by the present invention, the sample loading groove is arranged parallel to the short side wall of the bottom shell, the chromatography test paper is arranged parallel to the long side wall of the bottom shell, and a detection result display window is provided on the upper cover corresponding to the chromatography test paper. In this way, the size of the bottom shell can be fully utilized and the volume of the detection device can be reduced. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to more clearly illustrate the specific implementation methods of the present invention or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some implementation methods of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0026] Figure 1 is a three-dimensional perspective schematic diagram of a detection device in an embodiment of the present invention;

[0027] Figure 2 A three-dimensional perspective schematic diagram of a detection column in an embodiment of the present invention;

[0028] Figure 3 It is a three-dimensional perspective schematic diagram of the upper cover in an embodiment of the present invention;

[0029] Figure 4 is a three-dimensional perspective schematic diagram of the bottom shell in an embodiment of the present invention;

[0030] Figure 5 It is a three-dimensional schematic diagram of the extrusion block at a first angle in an embodiment of the present invention;

[0031] Figure 6 A perspective schematic diagram of an extrusion block at a second angle in an embodiment of the present invention;

[0032] Figure 7 It is a three-dimensional perspective schematic diagram of the cooperation between the buckle and the extrusion block in the embodiment of the present invention;

[0033] Figure 8 is a three-dimensional perspective schematic diagram of a buckle in an embodiment of the present invention;

[0034] Fig. 9 Schematic diagram of the internal weld of the mixing chamber in an embodiment of the present invention.

[0035] Description of reference numerals:

[0036] 1. Detection body; 11. Sealing cover; 12. Sample injection tube; 121. Rigid pipeline; 122. Flexible pipeline; 13. Nucleic acid extraction-free reagent chamber; 14. First buffer chamber; 15. Nucleic acid amplification reagent chamber; 16. Second buffer chamber; 17. Mixing chamber; 18. Sample loading tank; 178. Flexible bag tube; 171. Virtual welding seam; 172. Real welding seam; 19. Chromatographic test paper;

[0037] 2. Upper cover; 21. Result display window; 22. Upper slide; 23. Clamping structure;

[0038] 3. Bottom shell; 31. Partition wall; 32. Side slide groove; 33. First heating plate storage groove; 34. First battery storage groove; 35. First switch button; 36. Second heating plate storage groove; 37. Second battery storage groove; 38. Second switch button; 39. Test strip holder; 391. Test strip fixing piece; 310. Sample injection tube limiting hole; 312. First display light; 313. Second display light;

[0039] 4. Extrusion block; 41. Handle; 42. Neck; 421. Limiting protrusion; 44. Extrusion head;

[0040] 5. Buckle. DETAILED DESCRIPTION

[0041] The technical solution of the present invention will be described clearly and completely below in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0042] In the description of the present invention, it should be noted that the terms "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first" and "second" are only used for descriptive purposes and cannot be understood as indicating or implying relative importance.

[0043] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0044] In addition, the technical features involved in the different embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.

[0045] like Figures 1 to 9As shown, the present invention provides a nucleic acid integrated detection device (hereinafter referred to as the detection device) based on a flexible structure and a push-pull structure, and the detection device mainly includes a detection body 1, an upper cover 2, a bottom shell 3, an extrusion block 4 and a buckle 5. The detection body 1 is placed in the accommodating cavity between the bottom shell 3 and the upper cover 2.

[0046] The detection body 1 includes a sample injection tube 12, a nucleic acid extraction-free reagent chamber 13, a first buffer chamber 14, a nucleic acid amplification reagent chamber 15, a second buffer chamber 16, a mixing chamber 17, a sample loading tank 18, and a chromatography test paper 19 connected in sequence. Since the nucleic acid extraction-free reagent chamber 13, the first buffer chamber 14, the nucleic acid amplification reagent chamber 15, the second buffer chamber 16, and the mixing chamber 17 are all chambers made of flexible materials, they are collectively referred to as flexible reagent chambers. The number of flexible reagent chambers can be determined according to the required reaction steps and functions.

[0047] exist Figure 2 From the perspective of the embodiment, the sample injection tube 12 is arranged perpendicular to the short side wall on the left side of the bottom shell 3 for injecting the sample, and the outer end of the sample injection tube 12 is threadedly connected to the sealing cover 11 and sealed by the sealing cover 11. Figure 2 As shown, one end of the sample injection tube 12 located in the bottom shell 3 is connected to a rigid pipe 121 that is tilted downward toward the bottom of the bottom shell 3 to reduce the reagent residue in the rigid pipe 121. A flexible pipe 122 is connected to the end of the rigid pipe 121 away from the sample injection tube 12 so that the extrusion block 4 can squeeze the sample into the flexible reagent chamber. The pipeline connected to the end of the sample injection tube 12 located in the bottom shell 3 can also be a soft pipe. In this case, the length of the soft pipe is relatively large. In addition to being used for liquid samples, it can also be used for swab samples, and the sample loss can be reduced by squeezing the soft pipe by the swab. Before adding the sample, the sample injection tube 12 can be placed in a vertical upward state by changing the placement orientation of the entire device. In this way, in addition to facilitating the sample addition, it is also beneficial for the sample to enter the flexible reagent chamber smoothly.

[0048] The buffer solution in the first buffer chamber 14 is the buffer solution in the corresponding nucleic acid amplification system in the nucleic acid amplification reagent chamber 15. The nucleic acid amplification reagent chamber 15 can use constant temperature amplification techniques such as RPA and LAMP, or can use PCR variable temperature amplification technology. The volume of the nucleic acid amplification reagent chamber 15 is twice the volume of the liquid therein, so that the nucleic acid amplification system can be spread as flat as possible, increase the contact area with the heating plate described below, and make the liquid heated evenly. The reagent in the second buffer chamber 16 can be water or other diluents, which are used to dilute the amplified product before reaching the chromatography test paper 19 for detection.

[0049] When mixing reagents in each flexible reagent chamber, the entire device can be turned upside down 2-3 times to obtain a better mixing effect. The size of each flexible reagent chamber can be adjusted according to the volume of different reaction reagents, but the uniform length of the weld between the two adjacent flexible reagent chambers must be guaranteed, and the width of the weld must be reserved to avoid affecting the volume of each flexible reagent chamber. 1 / 3 of the length of each weld between the two adjacent flexible reagent chambers is welded by virtual welding technology, and the remaining 2 / 3 of the length is welded by ordinary thermal welding technology. It should be noted that the virtual welding weld located downstream can be opened by increasing the pressure of the liquid in the previous flexible reagent chamber, thereby achieving the mixing of the liquid in the two adjacent flexible reagent chambers, and the real welding weld will not be affected in this process. The flexible reagent chamber is made of flexible material, and materials such as polypropylene, polyethylene, polyurethane, and polyolefin copolymer can be selected.

[0050] exist Figure 2 From the perspective of the sample loading tank 18, the head end is connected to the mixing chamber 17 through a flexible bag tube 178 (the flexible bag tube 178 is essentially an extension of the mixing chamber 17), and a chromatography test paper 19 for detection is provided at the tail end of the sample loading tank 18. Figure 1 From a viewing angle, the sample loading slot 18 is arranged parallel to the short side wall on the right side of the bottom shell 3. The chromatography test paper 19 is arranged parallel to the long side wall of the bottom shell 3. The lower surface of the sample loading slot 18 is fixed to the inner bottom surface of the bottom shell 3 by double-sided tape to prevent it from shaking during the transportation of the detection body 1. The detection results are intuitively presented through the chromatography test paper 19, and different primer probe labels can be used to achieve multiple detections of the same system.

[0051] The upper cover 2 is provided with a result display window 21, an upper slide groove 22 for the neck 42 of the extrusion block 4 described below to slide, and a locking structure 23 (in this embodiment, the locking structure 23 is a circular groove, and in other embodiments, it can also be an elongated groove) that cooperates with the limiting protrusion 421 on the extrusion block 4. The locking structure 23 is arranged corresponding to the first buffer chamber 14. The width of the upper slide groove 22 is consistent with the width dimension at the neck 42 to prevent the extrusion block 4 from shaking left and right during the sliding process. The limiting protrusion 421 on the extrusion block 4 enters the preset locking structure 23, which can remind the operator that the extrusion block 4 has slid to the preset position, and there is no need to push the extrusion block 4 to continue to move to the right to control the reaction process.

[0052] A partition wall 31 is provided in the bottom shell 3. A side slide groove 32 is provided on the partition wall 31 on one side facing the extrusion head 44. The extrusion head 44 of the extrusion block 4 described below slides along the side slide groove 32 between the partition wall 31 and a long side wall of the bottom shell 3. The side slide groove 32 is "L"-shaped, and its height is consistent with the height of the extrusion head 44 of the extrusion block 4.

[0053] The bottom shell 3 is provided with a first heating plate storage slot 33 for storing the first heating plate, a first battery storage slot 34 for powering the first heating plate, a first switch button 35 and a first display light 312. The first heating plate storage slot 33 is arranged directly below the nucleic acid extraction-free reagent chamber 13.

[0054] The bottom shell 3 is also provided with a second heating plate storage slot 36 for storing the second heating plate, a second battery storage slot 37 for powering the second heating plate, a second switch button 38 and a second display light 313. The second heating plate storage slot 36 is arranged directly below the nucleic acid amplification reagent chamber 15.

[0055] Of course, the aforementioned heating plate can also be replaced by a heating paste or a self-heating bag. For the convenience of explanation, the heating plate, the heating paste and the self-heating bag are collectively referred to as a heating module. When the heating module is a heating paste and a self-heating bag, there is no need to set a battery. In addition, whether the heating module is set depends on whether the reaction system has a heating demand. When the heating module is not set, the first heating plate storage slot 33, the second heating plate storage slot 36, the first battery storage slot 34, and the second battery storage slot 37 can be retained. Of course, when the reaction system has no heating demand, the first heating plate storage slot 33, the second heating plate storage slot 36, the first battery storage slot 34, and the second battery storage slot 37 may not be set.

[0056] A test strip holder 39 is also provided at the bottom of the bottom shell 3. The test strip holder 39 is provided with a plurality of test strip fixing members 391 for fixing the chromatographic test strip 19 to prevent it from shifting during transportation or use.

[0057] An injection tube limiting hole 310 is provided on the short side wall of the bottom shell 3 adjacent to the injection tube 12 for fixing the injection tube 12 of the detection body 1 . The inner diameter of the injection tube limiting hole 310 is equivalent to the outer diameter of the injection tube 12 .

[0058] like Figure 5 and Figure 6 As shown, as an embodiment, the extrusion block 4 includes a handle 41, a neck 42 and an extrusion head 44 connected in sequence. The handle 41 can be of any shape and always protrudes from the top surface of the upper cover 2. A limiting protrusion 421 is provided on one side of the neck 42, and the limiting protrusion 421 cooperates with the following positioning structure 23. The extrusion head 44 is used to squeeze the flexible reagent chamber so that the upstream flexible reagent chamber is connected with the downstream flexible reagent chamber. Specifically, the extrusion head 44 is a thin wedge-shaped head, which is in a straight line. In the width direction of the flexible reagent chamber, the width of the extrusion head 44 at least completely covers the flexible reagent chamber, and the width value of the extrusion head 44 is equivalent to the spacing value between the side slide groove 32 described below and the corresponding long side wall of the bottom shell 3, so as to ensure that the extrusion block 4 can drive out the liquid in the corresponding flexible reagent chamber during the sliding process, and all enter the downstream flexible reagent chamber, and there will be no positional displacement.

[0059] like Figure 7 and Figure 8 As shown, the buckle 5 is U-shaped, and the opening of the buckle 5 faces the neck 42. The width of the buckle 5 is greater than the width of the upper slide 22, and less than the width of the handle 41, so that the buckle 5 can be stuck on the neck 42 of the extrusion block 4 without detaching from the extrusion block 4 upward. During transportation or before detection, the bottom surface of the buckle 5 is against the top surface of the upper cover 2, so that the extrusion head 44 of the extrusion block 4 is not in contact with the flexible pipe 122 and the flexible reagent chamber below, or although it is in contact, the extrusion force is small. When the extrusion head 44 is needed to extrude the flexible pipe 122 and the flexible reagent chamber, remove the buckle 5, press the extrusion block 4 downward to the bottom of the side slide 32, and slide the extrusion block 4. The first heating plate and the second heating plate are collectively referred to as heating plates.

[0060] like Fig. 9 As shown, virtual welds 171 are respectively provided at both ends of the mixing chamber 17. The virtual welds 171 will only open under the extrusion of the extrusion block 4. A plurality of real welds 172 are alternately arranged between the two virtual welds 171 to form a Z-shaped mixing flow channel in the mixing chamber 17 to enhance the mixing effect.

[0061] The specific operation process of the nucleic acid integrated detection device based on the flexible structure and push-pull structure in this embodiment is as follows:

[0062] Stand the detection device upright, make the sample injection tube 12 point vertically upward, add the sample, tighten the sealing cover 11, and then the sample flows into the nucleic acid extraction-free reagent chamber 13 and mixes with the nucleic acid extraction-free reagent;

[0063] Remove the buckle 5 and press the extrusion block 4 toward the bottom of the bottom shell 3 until the bottom of the extrusion head 44 abuts against the flexible pipe 122 to squeeze the sample in the flexible pipe 122 into the nucleic acid extraction-free reagent chamber 13;

[0064] The detection device is placed horizontally. Due to the obstruction of the squeezing block 4 , the mixed liquid in the nucleic acid-free extraction reagent chamber 13 will not flow back toward the injection tube 12 .

[0065] Turn on the first switch button 35, the first display light 312 lights up, the first heating plate heats the nucleic acid extraction-free reagent chamber 13 to a preset temperature, and the sample begins to lyse until the first display light 312 goes out;

[0066] The handle 41 is held by hand to push the extrusion block 4 to move away from the injection tube 12. During the sliding process of the extrusion block 4, the lysed solution in the nucleic acid-free extraction reagent chamber 13 and the RPA buffer in the first buffer chamber 14 enter the nucleic acid amplification reagent chamber 15 and are mixed with the RPA reagent in the nucleic acid amplification reagent chamber 15. When the limiting protrusion 421 on the extrusion block 4 coincides with the locking structure 23 and a sense of jamming occurs, the sliding of the extrusion block 4 is stopped.

[0067] Turn on the second switch button 38, the second display light 313 lights up, the second heating plate heats the nucleic acid amplification reagent chamber 15, and the sample undergoes an amplification reaction in the nucleic acid amplification reagent chamber 15 until the second display light 313 goes out, and the amplification is completed to obtain an amplification product;

[0068] Holding the handle 41, continue to push the squeezing block 4 in the direction away from the sample injection tube 12. The amplified product flows through the second buffer chamber 16 and is mixed and diluted with the diluent in the second buffer chamber 16, and then enters the mixing chamber 17 for mixing, and finally reaches the chromatography test paper 19 through the flexible bag tube 178 and the sample loading slot 18 for detection, and the detection result is output from the result display window 21.

[0069] The nucleic acid integrated detection device based on the flexible structure and push-pull structure provided by the present invention adopts a unique chamber isolation and fluid control technology to achieve rapid, simple and low-cost nucleic acid detection. Specifically, the device uses solid welds and virtual welds to construct different functional chambers and mixing channels in the detection body 1, and the solid welds can ensure the sealing and isolation between the chambers, while the virtual welds provide a controllable propulsion connection mechanism, allowing the virtual welds to be forced to open by physical means (through the extrusion of the extrusion block 4) when necessary to achieve communication between adjacent chambers.

[0070] In addition, the nucleic acid integrated detection device based on flexible structure and push-pull structure provided by the present invention adopts a slider extrusion mechanism (in this embodiment, the physical extrusion of the chamber by the extrusion block 4 promotes the flow of liquid), so that the reaction process can be controlled. The advantage of this design is its simplicity and directness, without relying on an external pumping system or complex valve control. The implementation of the slider extrusion mechanism promotes the continuous flow of liquid in the microchannel and between chambers by applying mechanical force in a specific area of ​​the flexible reagent chamber, ensuring the uniform mixing and rapid transfer of reactants. This design not only simplifies the fluid manipulation mechanism, reduces the manufacturing cost and operating difficulty of the equipment, but also expands the use environment of the detection system, making it easier to use in resource-limited areas or on-site rapid detection scenarios.

[0071] In addition, the design of the integrated nucleic acid detection device based on flexible structure and push-pull structure provided by the present invention fully considers the user experience and ease of operation, so that non-professionals can easily perform nucleic acid detection operations. Moreover, due to its small size and good portability, it can be used in a variety of application scenarios (including clinical laboratories, on-site testing, home self-testing, etc.), greatly expanding the accessibility and application scope of nucleic acid testing.

[0072] Obviously, the above embodiments are merely examples for the purpose of clear explanation, and are not intended to limit the implementation methods. For those skilled in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the implementation methods here. The obvious changes or modifications derived therefrom are still within the scope of protection of the invention.

Claims

1. A nucleic acid integrated detection device based on a flexible structure and a push-pull structure, characterized in that: It comprises a detection body (1), an upper cover (2), a bottom shell (3), and an extrusion block (4) installed between the upper cover (2) and the bottom shell (3); The detection body (1) is arranged in a containing cavity between the upper cover (2) and the bottom shell (3); the detection body (1) comprises an injection tube (12), a nucleic acid extraction-free reagent chamber (13), a first buffer chamber (14), a nucleic acid amplification reagent chamber (15), a second buffer chamber (16), a mixing chamber (17), a sample loading groove (18) and a chromatography test paper (19) which are connected in sequence; the nucleic acid extraction-free reagent chamber (13), the first buffer chamber (14), the nucleic acid amplification reagent chamber (15), the second buffer chamber (16) and the mixing chamber (17) are all flexible reagent chambers; the head end of the sample loading groove (18) is connected to the mixing chamber (17), and the tail end of the sample loading groove (18) is connected to the chromatography test paper (19); wherein; The squeezing block (4) can move from one end of the upper cover (2) to the other end, and is used to squeeze the liquid in the detection body (1), forcing the liquid to flow downstream.

2. The nucleic acid integrated detection device based on flexible structure and push-pull structure according to claim 1 is characterized in that: Two adjacent flexible reagent chambers are divided into two independent chambers by a weld, and one third of the length of the weld is a virtual weld.

3. The nucleic acid integrated detection device based on flexible structure and push-pull structure according to claim 1 is characterized in that: The upper cover (2) is provided with an upper slide groove (22), the bottom shell (3) is provided with a partition wall (31), the extrusion block (4) comprises a handle (41), a neck (42) and an extrusion head (44) which are connected in sequence, the handle (41) protrudes from the top surface of the upper cover (2), the neck (42) can slide along the upper slide groove (22), and the extrusion head (44) is located between the partition wall (31) and the long side wall of the bottom shell (3).

4. The nucleic acid integrated detection device based on flexible structure and push-pull structure according to claim 3 is characterized in that: A side sliding groove (32) is provided on the partition wall (31) on one side facing the extrusion head (44) and is recessed inwardly, and the extrusion head (44) slides along the side sliding groove (32).

5. The nucleic acid integrated detection device based on flexible structure and push-pull structure according to claim 3 is characterized in that: A locking structure (23) is recessed on the side wall of the upper slide groove (22) corresponding to the first buffer chamber (14), and a limiting protrusion (421) is provided on the neck (42), and the limiting protrusion (421) is engaged with the locking structure (23).

6. The nucleic acid integrated detection device based on flexible structure and push-pull structure according to claim 3 is characterized in that: The detection device further comprises a U-shaped buckle (5), the opening of the buckle (5) faces the neck (42) and is clamped on the neck (42), and the bottom surface of the buckle (5) abuts against the top surface of the upper cover (2), so that the squeezing block (4) does not exert a squeezing effect on the liquid in the detection body (1).

7. The nucleic acid integrated detection device based on a flexible structure and a push-pull structure according to any one of claims 1 to 6, characterized in that: The sample loading groove (18) is arranged parallel to the short side wall of the bottom shell (3), the chromatography test paper (19) is arranged parallel to the long side wall of the bottom shell (3), and a test result display window (21) is provided on the upper cover (2) corresponding to the chromatography test paper (19).

8. The nucleic acid integrated detection device based on flexible structure and push-pull structure according to claim 7, characterized in that: The head end of the sample injection tube (12) is clamped on a short side wall of the bottom shell (3) and protrudes from the short side wall, or the head end of the sample injection tube (12) is clamped on the top surface of the upper cover (2) and protrudes from the top surface of the upper cover (2).

9. The nucleic acid integrated detection device based on flexible structure and push-pull structure according to claim 8, characterized in that: The sample injection tube (12) is connected to the nucleic acid extraction-free reagent chamber (13) via a rigid pipe (121) and a flexible pipe (122) in sequence, and the rigid pipe (121) is arranged to be inclined toward the bottom wall of the bottom shell (3).

10. The nucleic acid integrated detection device based on a flexible structure and a push-pull structure according to any one of claims 1 to 6 or 8 or 9, characterized in that: It also includes two heating modules, which are used to heat the nucleic acid-free extraction reagent chamber (13) and the nucleic acid amplification reagent chamber (15) respectively.

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