Optical detection device, control method thereof and sample analyzer

Abstract

The invention discloses an optical detection device and a control method thereof, and a sample analyzer, in the optical detection device, a fluorescence excitation assembly is used for emitting an excitation light beam, and a bright / dark field illumination assembly is used for emitting an illumination light beam; the bearing table is located on a light emitting path of the excitation light beam and the illumination light beam; the objective lens is positioned on the lower side of the bearing table and is used for carrying out microscopic imaging on fluorescent particles in the to-be-detected sample to form a first imaging light beam, imaging the to-be-detected sample in a bright field to form a second imaging light beam and imaging the to-be-detected sample in a dark field to form a third imaging light beam; the imaging receiving assembly is located on a light emitting path of the objective lens and used for receiving the first imaging light beam, the second imaging light beam and the third imaging light beam. The optical detection device can perform fluorescence detection on a sample, can perform bright / dark field detection on the sample, is simple and compact in structure, low in cost and clear in imaging effect, and can improve the accuracy of sample detection.

Description

Technical Field

[0001] The present application relates to the technical field of medical equipment, and in particular to an optical detection device and a control method thereof, and a sample analyzer. Background Art

[0002] Blood component testing mainly includes red blood cell testing, white blood cell testing and platelet testing. Blood component testing is an important clinical medical indicator. Optical testing is commonly used for testing blood components. Different optical testing devices are generally used for different testing items.

[0003] In the related art, the optical detection device of the sample analyzer generally adopts an infinite microscope system for imaging. The cost of optical components is relatively high, and the detection items are single, making it difficult to switch between multiple detection modes. Summary of the invention

[0004] The present application provides an optical detection device and a control method thereof, and a sample analyzer to solve the technical problems in the prior art that the optical components are relatively high in cost, the detection items are single, and it is difficult to switch between multiple detection modes.

[0005] In order to solve the above technical problems, a technical solution adopted in the present application is: to provide an optical detection device, which includes: a fluorescence excitation component and a bright / dark field illumination component arranged at intervals, a carrier platform, an objective lens and an imaging receiving component, the fluorescence excitation component is used to emit an excitation light beam, and the bright / dark field illumination component is used to emit an illumination light beam; the carrier platform is located on the light output path of the excitation light beam and the illumination light beam, and the carrier platform is used to carry a sample to be detected; the objective lens is located on the lower side of the carrier platform, and is used to perform microscopic imaging of fluorescent particles in the sample to be detected to form a first imaging light beam, and is used to image the sample to be detected under the bright field to form a second imaging light beam, and to image the sample to be detected under the dark field to form a third imaging light beam; the imaging receiving component is located on a light output path of the objective lens, and is used to receive the first imaging light beam, the second imaging light beam and the third imaging light beam.

[0006] Furthermore, the imaging receiving component includes: a light selecting component and a receiver, the receiver is located on the light output path of the light selecting component, the light selecting component includes at least two light selecting sub-components connected in sequence, and the at least two light selecting sub-components are movably arranged so that at least two light selecting sub-components can be selectively moved between the objective lens and the receiver for selecting the imaging mode of the first imaging light beam, the second imaging light beam or the third imaging, and the receiver is used to receive the imaging light beam of the preset mode.

[0007] Furthermore, at least two photoselective components include a cavity body and at least one filter connected in sequence, the cavity body is formed with an empty hole, the empty hole is used to allow the first imaging beam and / or the second imaging beam to pass directly, and the filter is used to filter the first imaging beam.

[0008] Furthermore, the bright / dark field lighting assembly includes: an illumination light source for emitting an illumination light beam; a collimating lens located on the light output path of the illumination light source and used to collimate the illumination light beam; a shading member provided on the light output path of the collimating lens and used to block the illumination light beam within a preset range; and a first condensing lens located on the light output path of the shading member and used to focus the illumination light beam.

[0009] Furthermore, the bright / dark field lighting assembly includes: an illumination light source for emitting an illumination light beam; a collimating lens located on the light output path of the illumination light source for collimating the illumination light beam; and a first condensing lens located on the light output path of the collimating lens for focusing the collimated illumination light beam.

[0010] Furthermore, the bright / dark field illumination assembly is movably disposed above the carrier platform in the vertical direction so that the bright / dark field illumination assembly has a first state and a second state. In the first state, the first condensing lens is used to focus the collimated illumination light beam on the pupil of the objective lens; in the second state, a shading member is provided between the first condensing lens and the collimating lens, the shading member is used to block the illumination light beam within a preset range, and the first condensing lens is used to focus the illumination light beam passing through the shading member on the sample to be detected.

[0011] Furthermore, the bright / dark field lighting assembly also includes: a light homogenizer, which is arranged on the light output path of the collimating lens and is used to homogenize the collimated illumination light beam.

[0012] Furthermore, the optical detection device also includes: a spectroscopic component, which is located on the light output path of the fluorescent excitation component, and is used to emit the excitation light beam to the objective lens, and the objective lens is used to collimate the excitation light beam and then emit it to the sample to be detected. The spectroscopic component is also used to guide the first imaging light beam, the second imaging light beam and the third imaging light beam emitted by the objective lens to the imaging receiving component.

[0013] In order to solve the above technical problems, a technical solution adopted in the present application is: to provide a control method for an optical detection device, the control method is based on the optical detection device of any of the above embodiments, specifically, the control method includes: placing the sample to be tested on a carrier table; emitting an excitation beam through a fluorescent excitation component, and emitting an illumination beam through a bright / dark field illumination component, wherein the light emission paths of the excitation beam and the illumination beam pass through the sample to be tested; microscopically imaging the fluorescent particles in the sample to be tested through an objective lens to form a first imaging beam, imaging the sample to be tested in a bright field through the objective lens to form a second imaging beam, and imaging the sample to be tested in a dark field through the objective lens to form a third imaging beam; receiving the first imaging beam, the second imaging beam and the third imaging beam through an imaging receiving component.

[0014] In order to solve the above technical problems, a technical solution adopted in the present application is: to provide a sample analyzer, which includes: a sample preparation device and an optical detection device of any of the above embodiments, the sample preparation device is used to prepare the sample to obtain a sample to be detected; the optical detection device is used to perform optical detection on the sample to be detected.

[0015] Beneficial effects of the present application: Different from the prior art, the optical detection device provided by the present application includes: a fluorescence excitation component and a bright / dark field illumination component arranged at intervals, a carrier, an objective lens and an imaging receiving component. The sample to be detected on the carrier can generate fluorescence under the action of the excitation light. The objective lens can be used to perform microscopic imaging of the fluorescent particles in the sample to be detected to form a first imaging beam, and can also image the sample to be detected in a bright field to form a second imaging beam, and image the sample to be detected in a dark environment to form a third imaging beam. The imaging receiving component is used to receive the first imaging beam, the second imaging beam and the third imaging beam to obtain an image of the sample to be detected. The optical detection device in the present application has a simple and compact structure and low cost. It can perform fluorescence detection on the sample to be detected and can also perform bright / dark field detection on the sample to be detected. That is, the optical detection device can perform detection of multiple items, the optical imaging effect is clear, and the detection reliability is high. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings required for use in the description of the embodiments are briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For those skilled in the art, other drawings can be obtained based on these drawings without creative work, among which:

[0017] Figure 1 is a structural schematic diagram of an embodiment of an optical detection device provided by the present application;

[0018] Figure 2 is a structural schematic diagram of another embodiment of the optical detection device provided by the present application;

[0019] Figure 3 is a flow chart of an embodiment of a control method for an optical detection device provided by the present application;

[0020] Figure 4 It is a structural schematic diagram of an embodiment of a sample analyzer provided by the present application. DETAILED DESCRIPTION

[0021] In order to make the above-mentioned purposes, features and advantages of the present application more obvious and easy to understand, the specific implementation methods of the present application are described in detail below in conjunction with the accompanying drawings. It is to be understood that the specific embodiments described herein are only used to explain the present application, rather than to limit the present application. It should also be noted that, for ease of description, only some structures related to the present application are shown in the accompanying drawings, rather than all structures. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of this application.

[0022] The terms "first", "second", etc. in this application are used to distinguish different objects, rather than to describe a specific order. In addition, the terms "including" and "having" and any variations thereof are intended to cover non-exclusive inclusions. For example, a process, method, system, product or device that includes a series of steps or units is not limited to the listed steps or units, but optionally includes steps or units that are not listed, or optionally includes other steps or units inherent to these processes, methods, products or devices.

[0023] Reference to "embodiments" herein means that a particular feature, structure, or characteristic described in conjunction with the embodiments may be included in at least one embodiment of the present application. The appearance of the phrase in various locations in the specification does not necessarily refer to the same embodiment, nor is it an independent or alternative embodiment that is mutually exclusive with other embodiments. It is explicitly and implicitly understood by those skilled in the art that the embodiments described herein may be combined with other embodiments.

[0024] The present application provides an optical detection device, which can be used for fluorescence detection of samples to be detected, and can also detect samples to be detected in bright / dark fields, thereby expanding the detection range of the optical detection device. In addition, the optical detection device provided by the present application has a simple and compact structure, low cost, clear optical imaging effect of the samples to be detected, and high reliability and strong practicality.

[0025] See also Figure 1 As shown, Figure 1 1 is a schematic diagram of the structure of an embodiment of the optical detection device provided by the present application. Specifically, the optical detection device 10 includes: a fluorescence excitation component 11, a bright / dark field illumination component 12, a carrier 13, an objective lens 14 and an imaging receiving component 15 arranged at intervals. Optionally, the optical detection device 10 may also include a spectroscopic component 16.

[0026] The carrier 13 is used to carry the sample to be tested (not shown in the figure), which can be a blood sample, sweat sample or saliva sample after specific treatment. In a specific embodiment, when performing fluorescence detection on a blood sample, the blood sample can be stained with acridine orange dye solution in a ratio of 1:1 for 4 minutes to obtain the sample to be tested, and then 5 microliters of the sample to be tested can be drawn with a pipette and dropped on the center of the slide, and then a cover glass is used to press positively on the droplet of the sample to be tested at a small acute angle to make the droplet spread evenly. After the diffusion is completed, the carrier is placed on the carrier 13 to be optically detected by the optical detection device 10. In other embodiments, the sample can also be processed in other ways according to the requirements of the detection project to obtain the sample to be tested, such as diluting with a diluent, etc., which are not listed here one by one.

[0027] The fluorescence excitation component 11 is used to emit an excitation light beam, and the bright / dark field illumination component 12 is used to emit an illumination light beam. The carrier 13 is located on the light exit path of the excitation light beam and the illumination light beam. Specifically, the excitation light beam emitted by the fluorescence excitation component 11 can be emitted to the sample to be detected on the carrier 13, and the fluorescent particles in the sample to be detected can be excited to emit a fluorescent light beam under the action of the excitation light beam. The illumination light beam emitted by the bright / dark field illumination component 12 is emitted to the sample to be detected on the carrier 13, and the sample to be detected can transmit the illumination light beam.

[0028] The objective lens 14 is located below the carrier 13, and is used to perform microscopic imaging of fluorescent particles in the sample to be detected to form a first imaging beam, and to perform imaging of the sample to be detected in a bright field to form a second imaging beam, and to perform imaging of the sample to be detected in a dark field to form a third imaging beam. That is, the objective lens 14 is used to magnify and image the sample to be detected. The objective lens 14 can be used for both fluorescence detection and bright / dark field detection, and has a simple and compact structure and low cost.

[0029] The imaging receiving component 15 is located on a light outgoing path of the objective lens 14 and is used to receive the first imaging light beam, the second imaging light beam and the third imaging light beam. In other words, the imaging receiving component 15 is used to receive the image formed by the objective lens 14 on the sample to be detected.

[0030] In the present application, a fluorescence detection system can be composed of a fluorescence excitation component 11, an objective lens 14 and an imaging receiving component 15. The excitation light beam emitted by the fluorescence excitation component 11 is emitted to the sample to be detected, and the sample to be detected is excited to produce fluorescence. The fluorescent particles are imaged by the objective lens 14 to form a first imaging light beam. The imaging receiving component 15 receives the first imaging light beam to obtain an image of the sample to be detected, and fluorescence detection and analysis of the sample to be detected is performed based on the imaging.

[0031] A bright / dark field detection system can be formed by a bright / dark field lighting component 12, an objective lens 14 and an imaging receiving component 15. The illumination light beam emitted by the bright / dark field lighting component 12 is emitted to the sample to be detected. In the bright field, the objective lens 14 images the sample to be detected to form a second imaging light beam. In the dark field, the objective lens 14 images the sample to be detected to form a third imaging light beam, so as to obtain an image of the sample to be detected, and a bright / dark field detection and analysis is performed on the sample to be detected based on the imaging.

[0032] Therefore, the optical detection device 10 of the present application integrates the fluorescence detection system and the bright / dark field detection system, and can perform both fluorescence detection and bright / dark field detection on the sample to be detected, so that the optical detection device 10 can perform multiple project detections, thereby improving the scope of application of the optical detection device 10. The fluorescence detection system and the bright / dark field detection system can share components such as the objective lens 14 and the imaging receiving assembly 15, thereby simplifying the structure of the optical detection device 10 and saving the cost of the optical detection device 10. Moreover, the present application adopts a finite distance system for imaging, and the imaging effect is clear, which can improve the accuracy of the detection of the sample to be detected.

[0033] Furthermore, if Figure 1 As shown, the imaging receiving assembly 15 includes a light selecting element 151 and a receiver 152. The receiver 152 is located on the light output path of the light selecting element 151, and the light selecting element 151 is used to select the imaging mode of the first imaging light beam, the second imaging light beam and the third imaging light beam.

[0034] Specifically, the light selection component 151 may include at least two light selection components (not marked) connected in sequence. The light selection component 151 is movably arranged so that one of the at least two light selection components is selected to move between the objective lens 14 and the receiver 152, so as to select the imaging mode of the first imaging light beam, the second imaging light beam and the third imaging light beam. That is, during the movement of the light selection component 151, the required light selection component can be located between the objective lens 14 and the receiver 152 to select the mode of the imaging light beam. A plurality of light selection components can be arranged side by side in a straight line, and the light selection component 151 can reciprocate in a straight line direction to switch the light selection components. In other embodiments, a plurality of light selection components can also be arranged in a circle, and the circular light selection component 151 rotates to switch the light selection components.

[0035] exist Figure 1In the illustrated embodiment, the light selection component 151 includes a light selection sub-component 1511, a light selection sub-component 1512, a light selection sub-component 1513, and a light selection sub-component 1514. When the optical detection device 10 performs classification and counting of white blood cells, red blood cells, and platelets on a blood sample, the light selection sub-component 1511 may be a green filter, the light selection sub-component 1512 may be a red filter, the light selection sub-component 1513 may be a long-pass filter, and the light selection sub-component 1514 may be a cavity. When it is necessary to observe red fluorescent cells, the light selection component 151 is moved to switch to the red filter, when it is necessary to observe green fluorescent cells, the light selection component 151 is moved to switch to the green filter, and when it is necessary to observe red and green fluorescent cells at the same time, the light selection component 151 is moved to switch to the long-pass filter. For example, the transmission band of the long-pass filter may be: 510-690nm. The filter filters the first imaging light beam. The cavity is formed with an empty hole (not shown) for allowing the second imaging beam or the third imaging beam to pass directly. It is understandable that the number and type of filters in the optical selection element 151 can be specifically set according to the requirements of the detection project, and are not specifically limited here.

[0036] In other embodiments, the light selection element 151 may also include only a cavity and a long-pass filter to simplify the structure of the light selection element 151 and save costs.

[0037] The receiver 152 is located on the light path of the light selection element 151 and is used to receive the imaging light beam of the preset mode. The receiver 152 can be a camera module, for example, the receiver 152 can be a CMOS (Complementary Metal Oxide Semiconductor) camera.

[0038] Furthermore, if Figure 1 As shown, the optical detection device 10 may further include: a spectroscopic component 16, the spectroscopic component 16 is located on the light output path of the fluorescent excitation component 11, the spectroscopic component 16 is used to guide the excitation light beam to the objective lens 14, the objective lens 14 is used to collimate the excitation light beam and then emit it to the sample to be detected, that is, the excitation light beam is emitted from the objective lens 14 as collimated light, so as to achieve uniform excitation, thereby improving the accuracy of sample detection.

[0039] The light splitting component 16 is also used to guide the first imaging light beam, the second imaging light beam and the third imaging light beam emitted by the objective lens 14 to the imaging receiving component 15. By using the light splitting component 16, the structure of the optical detection device 10 is more compact.

[0040] exist Figure 1In the illustrated embodiment, the spectroscopic component 16 can reflect the excitation beam into the objective lens 14, and the spectroscopic component 16 can allow the first imaging beam, the second imaging beam, and the third imaging beam to pass through the imaging receiving component 15. It is understood that in other embodiments, the spectroscopic component 16 can also reflect the first imaging beam, the second imaging beam, and the third imaging beam to the imaging receiving component 15, and pass the laser beam into the objective lens 14. In this way, the structure of the optical detection device 10 can be made more compact.

[0041] exist Figure 1 In the illustrated embodiment, the beam splitting component 16 is a dichroic mirror and an aperture. The dichroic mirror is used to filter the first imaging light beam, and the aperture is used to allow the second imaging light beam or the third imaging light beam to pass through.

[0042] Furthermore, if Figure 1 As shown, the fluorescence excitation component 11 includes: an excitation light source 111, an aperture 113, a second condensing lens 112 and a filter 114 arranged in sequence.

[0043] The excitation light source 111 is used to emit an excitation light beam. Specifically, a light emitting diode can be used as the excitation light source 111. For example, when counting white blood cells and platelets in a blood sample, a blue light emitting diode can be used as the excitation light source 111, and the output wavelength is 470nm±20nm. The filter 114 can be a blue filter that allows 470nm blue light to pass through. In other embodiments, a green light emitting diode can also be used as the excitation light source 111.

[0044] The aperture 113 can be used to reduce the divergence angle of the light beam and block stray light. The excitation light beam emitted by the excitation light source 111 passes through the aperture 113 to filter out stray light with a large divergence angle, and then is converged by the second condenser lens 112, and then filtered by the filter 114 to allow the excitation light beam of a specific wavelength to pass through. The excitation light beam of a specific wavelength is reflected to the rear focus of the objective lens 14 through the beam splitter 16, so that the excitation light beam is emitted from the objective lens 14 as collimated light, thereby achieving uniform excitation of the sample to be detected.

[0045] Furthermore, the geometric diameters of the aperture 113, the second condensing lens 112, and the filter 114 are the same, and the excitation light source 111, the aperture 113, the second condensing lens 112, the filter 114, the beam splitter component 16, and the objective lens 14 can be coaxially placed to improve the utilization of light.

[0046] In other embodiments, the fluorescence excitation component 11 may also only include the excitation light source 111 and the second condensing lens 112, the second condensing lens 112 is arranged on the light output path of the excitation light source 111, and the second condensing lens 112 is used to converge the excitation light beam, and the converged excitation light beam is guided to the rear focus of the objective lens 14 through the light splitting component 16 to simplify the structure of the fluorescence excitation component 11. The structure of the fluorescence excitation component 11 can be selected by conventional optical devices according to the actual detection items, and they are not listed one by one here.

[0047] Furthermore, if Figure 1 As shown, the bright / dark field illumination assembly 12 may include: an illumination light source 121 , a collimating lens 122 , and a first condensing lens 123 , which are arranged in sequence.

[0048] The illumination light source 121 is used to emit an illumination light beam, and the illumination light source 121 may be a white light emitting diode. In other embodiments, the illumination light source 121 may also be a yellow light emitting diode, a red light emitting diode, or a green light emitting diode (eg, with an output wavelength of 518±18 nm).

[0049] The collimating lens 122 is located on the light path of the illumination light source 121 and is used to collimate the illumination beam. Optionally, a light homogenizer 125 may be provided on the light path of the collimating lens 122 to homogenize the collimated illumination beam so that the illumination beam has a uniform incident light intensity.

[0050] The first condenser lens 123 is used to focus the collimated illumination beam. Preferably, the first condenser lens 123 can be configured to focus the collimated illumination beam on the pupil of the objective lens 14. After the objective lens 14 processes the light, the image plane has uniform brightness, thereby improving the imaging effect and the accuracy of the sample analyzer detection.

[0051] In this embodiment, the illumination light source 121, the collimating lens 122, the light homogenizer 125, the first condensing lens 123, and the objective lens 14 can be coaxially placed to improve the utilization of light. It is understandable that the bright / dark field illumination assembly 12 may also not be provided with the light homogenizer 125 to simplify the structure of the bright / dark field illumination assembly 12.

[0052] The structure of the bright / dark field illumination assembly 12 can detect samples to be detected in a bright field, such as detecting red blood cells in a blood sample. The bright field detection system has a simple structure, low cost, and clear imaging, which can improve the accuracy of sample detection.

[0053] In another embodiment, Figure 2 As shown, Figure 2It is a structural schematic diagram of another embodiment of the optical detection device provided in the present application. Different from the above-mentioned bright field illumination embodiment, in this embodiment, a shading member 124 is arranged on the light output path of the collimating lens 122, and the shading member 124 is used to block the illumination light beam within a preset range so that the scattered light of the illumination light source 121 cannot enter the field of view of the objective lens 14, thereby providing a dark field environment for the detection of the sample to be detected.

[0054] In this embodiment, the illumination light beam emitted by the illumination light source 121 is collimated by the collimating lens 122, and then the light intensity of the illumination light beam can be uniformed through the light homogenizer 125, and then through the shading member 124, and then through the first condensing lens 123, so that the illumination light beam is focused on the sample to be detected, that is, the glass slide on the support platform 13 is located at the focal plane of the first condensing lens 123, and the position of the focal plane is the object plane of the objective lens 14, providing a dark field environment for imaging of the sample to be detected.

[0055] In this embodiment, the scattered light of the illumination light source 121 cannot enter the field of view of the objective lens 14, thereby forming a dark field detection system. In this dark field detection system, the scattered light of the illuminated sample to be detected is magnified and imaged by the objective lens 14, and guided to the imaging receiving component 15 through the light splitting component 16. For example, the dark field detection system can be used to classify and count red blood cells and platelets in a blood sample.

[0056] In this embodiment, the light homogenizer 125 and the light shielding member 124 have the same geometric dimensions, and the collimating lens 122 and the first condensing lens 123 have the same geometric dimensions. The illumination light source 121, the collimating lens 122, the light homogenizer 125, the light shielding member 124, the first condensing lens 123, and the objective lens 14 can be coaxially placed to improve the utilization of light.

[0057] Further, in some embodiments, the bright / dark field lighting assembly 12 is movably disposed above the carrier 13 in a vertical direction, so that the bright / dark field lighting assembly 12 has a first state and a second state. Specifically, the bright / dark field lighting assembly 12 can move in a direction close to and away from the carrier 13, so that the bright / dark field lighting assembly 12 switches between the first state and the second state.

[0058] In the first state, the light shielding member 124 is not provided on the bright / dark field illumination assembly 12, and the light shielding member 12 is close to the carrier platform 13, so that the first focusing lens 123 can focus the collimated illumination light beam on the pupil of the objective lens 14, and the objective lens 14 performs microscopic imaging on the sample to be tested with uniform illumination of the sample area to form a second imaging light beam for bright field detection.

[0059] In the second state, the light shielding member 124 is provided in the bright / dark field lighting assembly 12. Specifically, the light shielding member 124 is provided between the first focusing lens 123 and the collimating lens 122. The light shielding member 124 is used to shield the illumination light beam within a preset range. The bright / dark field lighting assembly 12 is away from the carrier platform 13. The illumination light beam emitted by the bright / dark field lighting assembly 12 is shielded by the light shielding member 124 and focused on the sample to be tested. The light beam passing through the sample to be tested cannot enter the objective lens 14. The illuminated sample to be tested is imaged through the objective lens 14 to form a third imaging light beam for dark field detection.

[0060] By making the bright / dark field lighting assembly 12 movably arranged, for example, the bright / dark field lighting assembly 12 is adjustable up and down in the vertical direction, it is possible to achieve switching between bright / dark fields, thereby improving the flexibility of the use of the optical detection device 10 and further improving the applicability of the optical detection device 10.

[0061] The optical detection device 10 of the present application has a simple and compact structure, low cost, clear optical imaging effect, and can improve the accuracy of sample detection. Moreover, the optical detection device 10 can perform both fluorescence detection and bright / dark field detection on the sample to be detected, and the optical detection device 10 has a wide range of applications.

[0062] The present application also provides a control method for an optical detection device, the control method is based on the optical detection device 10 of any of the above embodiments. Figure 3 As shown, the control method includes:

[0063] S11: placing the sample to be tested on the carrying platform.

[0064] S12: an excitation beam is emitted through the fluorescence excitation component, and an illumination beam is emitted through the bright / dark field illumination component, wherein the light emission paths of the excitation beam and the illumination beam pass through the sample to be measured.

[0065] S13: Microscopically image the fluorescent particles in the sample to be tested through the objective lens to form a first imaging beam, image the sample to be tested in a bright field through the objective lens to form a second imaging beam, and image the sample to be tested in a dark field through the objective lens to form a third imaging beam.

[0066] S14: receiving the first imaging light beam, the second imaging light beam and the third imaging light beam through the imaging receiving component.

[0067] When the optical detection device of the present application detects the sample to be detected, the sample to be detected is first placed on a carrier, and a fluorescence detection system is formed by a fluorescence excitation component, an objective lens, and an imaging receiving component. The excitation light beam emitted by the fluorescence excitation component is emitted to the sample to be detected, and the sample to be detected is excited to produce fluorescence. The fluorescent particles are imaged by the objective lens to form a first imaging light beam, and the imaging receiving component receives the first imaging light beam to obtain an image of the sample to be detected, and fluorescence detection and analysis are performed on the sample to be detected based on the imaging. A bright / dark field illumination component, an objective lens, and an imaging receiving component form a bright / dark field detection system, and an illumination light beam emitted by the bright / dark field illumination component is emitted to the sample to be detected. In the bright field, the objective lens images the sample to be detected to form a second imaging light beam, and in the dark field, the objective lens images the sample to be detected to form a third imaging light beam to obtain an image of the sample to be detected, and bright / dark field detection and analysis are performed on the sample to be detected based on the imaging.

[0068] Regarding the specific detection methods of the fluorescence detection system and the bright / dark field detection system, please refer to the introduction of the above embodiments, which will not be repeated here.

[0069] The optical detection device of the present application can perform both fluorescence detection and bright / dark field detection on the sample to be detected, so that the optical detection device can perform multiple item detections, thereby improving the application scope of the optical detection device.

[0070] The present application also provides a sample analyzer, such as Figure 4 As shown, Figure 4 It is a structural schematic diagram of an embodiment of a sample analyzer provided in the present application. Specifically, the sample analyzer 30 includes: a sample preparation device 31 and an optical detection device 32. The sample preparation device 31 is used to prepare the sample to obtain a sample to be detected, and the optical detection device 32 is used to perform optical detection on the sample to be detected.

[0071] The optical detection device 32 of this embodiment may be the optical detection device 10 of any of the above embodiments. For the structure of the optical detection device 32 , please refer to the introduction of any of the above embodiments, which will not be repeated here.

[0072] The sample analyzer 30 in the present application may be a blood analyzer or a body fluid analyzer, etc. The test sample of the sample analyzer 30 may be blood, such as animal blood or human blood, etc., and the test sample may also be body fluids such as saliva, sweat or urine. The sample analyzer 30 of the present application can perform multiple tests on the sample, has a wide range of applications, and has a simple structure and low cost.

[0073] The above description is only an implementation method of the present application, and does not limit the patent scope of the present application. Any equivalent structure or equivalent process transformation made using the contents of the present application specification and drawings, or directly or indirectly used in other related technical fields, are also included in the patent protection scope of the present application.

Claims

1. An optical detection device, characterized in that: The optical detection device comprises: A fluorescence excitation component and a bright / dark field illumination component are arranged at intervals, wherein the fluorescence excitation component is used to emit an excitation light beam, and the bright / dark field illumination component is used to emit an illumination light beam; A carrying platform, located on the light exit path of the excitation light beam and the illumination light beam, and the carrying platform is used to carry the sample to be detected; an objective lens, located at the lower side of the carrier, for performing microscopic imaging of the fluorescent particles in the sample to be detected to form a first imaging beam, for imaging the sample to be detected in a bright field to form a second imaging beam, and for imaging the sample to be detected in a dark field to form a third imaging beam; The imaging receiving component is located on a light output path of the objective lens and is used for receiving the first imaging light beam, the second imaging light beam and the third imaging light beam.

2. The optical detection device according to claim 1, characterized in that: The imaging receiving assembly comprises: a light selecting element and a receiver, wherein the receiver is located on the light output path of the light selecting element. The light selection component includes at least two light selection sub-components connected in sequence, and the at least two light selection sub-components are movably arranged so that one of the at least two light selection sub-components can be moved between the objective lens and the receiver to select the imaging mode of the first imaging beam, the second imaging beam or the third imaging, and the receiver is used to receive the imaging beam of a preset mode.

3. The optical detection device according to claim 2, characterized in that: The at least two light selection components include a cavity body and at least one filter connected in sequence, The cavity is formed with an empty hole, and the empty hole is used to allow the first imaging light beam and / or the second imaging light beam to pass directly, and the filter is used to filter the first imaging light beam.

4. The optical detection device according to claim 1, characterized in that: The bright / dark field lighting assembly comprises: An illumination light source, for emitting an illumination light beam; A collimating lens, located on the light output path of the illumination light source, and used for collimating the illumination light beam; A light shielding member, arranged on the light output path of the collimating lens, and used for shielding the illumination light beam within a preset range; The first condensing lens is located on the light-emitting path of the shading element and is used for focusing the illumination light beam.

5. The optical detection device according to claim 1, characterized in that: The bright / dark field lighting assembly comprises: An illumination light source, for emitting an illumination light beam; A collimating lens, located on the light output path of the illumination light source, and used for collimating the illumination light beam; The first condensing lens is located on the light output path of the collimating lens and is used for focusing the collimated illumination light beam.

6. The optical detection device according to claim 5, characterized in that: The bright / dark field lighting assembly is movably disposed above the carrier in a vertical direction, so that the bright / dark field lighting assembly has a first state and a second state. In the first state, the first condenser lens is used to focus the collimated illumination light beam on the pupil of the objective lens; In the second state, a shading member is provided between the first condensing lens and the collimating lens, the shading member is used to shield the illumination light beam within a preset range, and the first condensing lens is used to focus the illumination light beam passing through the shading member on the sample to be detected.

7. The optical detection device according to claim 5 or 6, characterized in that: The bright / dark field lighting assembly also includes: The light homogenizer is arranged on the light output path of the collimating lens and is used to homogenize the collimated illumination light beam.

8. The optical detection device according to claim 1, characterized in that: The optical detection device also includes: A spectroscopic component, wherein the spectroscopic component is located on the light output path of the fluorescent excitation component, and the spectroscopic component is used to emit the excitation light beam to the objective lens, and the objective lens is used to collimate the excitation light beam and then emit it to the sample to be detected. The spectroscopic component is also used to guide the first imaging light beam, the second imaging light beam and the third imaging light beam emitted by the objective lens to the imaging receiving component.

9. A control method for an optical detection device, characterized in that: Based on the optical detection device according to any one of claims 1 to 8, the control method comprises: Place the sample to be tested on the carrier; An excitation light beam is emitted through a fluorescence excitation component, and an illumination light beam is emitted through a bright / dark field illumination component, wherein the light emission paths of the excitation light beam and the illumination light beam pass through the sample to be tested; Performing microscopic imaging of the fluorescent particles in the sample to be tested through an objective lens to form a first imaging beam, performing imaging of the sample to be tested in a bright field through the objective lens to form a second imaging beam, and performing imaging of the sample to be tested in a dark field through the objective lens to form a third imaging beam; The first imaging light beam, the second imaging light beam and the third imaging light beam are received by the imaging receiving component.

10. A sample analyzer, characterized in that: The sample analyzer comprises: A sample preparation device, used for preparing samples to obtain samples to be tested; The optical detection device according to any one of claims 1 to 8 is used to perform optical detection on the sample to be detected.

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