Sunscreen additive composition and application thereof

Abstract

The invention relates to the technical field of daily chemicals, in particular to a sunscreen additive composition and application thereof. The composition comprises a roxburgh rose fruit extract and a cactus extract. The composition provided by the invention has very strong oxidation resistance, no preservative is added, and the composition is multi-skin and zero-irritation; and SPF60 PA < + + + + > can be reached under the condition that the number of the chemical sun-screening agents is not more than 3.

Description

Technical Field

[0001] The present invention relates to the technical field of daily chemicals, and in particular to a sunscreen additive composition and application thereof. Background Art

[0002] At present, the market demand for high-factor sunscreen products is getting stronger and stronger, but most high-factor (for example, SPF60PA++++) sunscreen products rely on a large number of and a variety of chemical sunscreens and physical sunscreens to achieve the sun protection index. Although physical sunscreens are not easily absorbed by the skin, most consumers apply sunscreen products on their faces for a long time. Too much chemical sunscreen and physical sunscreen will make the skin airtight and too many chemical ingredients will cause people with sensitive skin to be prone to allergies.

[0003] Although physical sunscreens are not absorbed by the skin, in order to achieve sunscreen effects, more and more researchers are now applying nano-inorganic materials to physical sunscreens. However, as in-depth research has found that nano-inorganic materials are easily absorbed by the skin due to their small particle size and long-term presence on the skin surface, so there is now opposition to nano-inorganic materials.

[0004] At present, high-factor sunscreen requires reducing the content of chemical sunscreens as much as possible, and using biological sunscreen additives to increase the SPF index of the basic formula. However, the current biological sunscreen solution has a relatively low degree of improvement in the SPF index and cannot replace chemical sunscreens to a large extent.

[0005] In response to the above problem, the present invention provides a sunscreen additive composition that can achieve a relatively good sunscreen effect and can increase the SPF of existing sunscreen products of SPF50 to SPF60. Summary of the invention

[0006] The object of the present invention is to provide a sunscreen additive composition, which comprises: roxburghii fruit extract and cactus extract.

[0007] As an embodiment of the present invention, the composition further comprises: SOD.

[0008] As an embodiment of the present invention, the composition comprises 75 parts of roxburghii fruit extract, 10 parts of cactus extract, and 5 parts of SOD in terms of mass ratio.

[0009] As an embodiment of the present invention, the roxburghii fruit extract is prepared by fermenting roxburghii fruit.

[0010] As an embodiment of the present invention, the cactus extract is prepared by fermenting cactus.

[0011] As an embodiment of the present invention, the strains of bacteria used to ferment the roxburghii fruit are: Lactobacillus acidophilus and Bacillus licheniformis.

[0012] As an embodiment of the present invention, the culture medium components of the fermented cactus are: 5.0 g of peptone, 3.0 g of beef extract powder, 5.0 g of NaCl, 15.0 g of oatmeal powder, 1.0 g of Tween 80, 5.0 g of sodium acetate, 2.0 g of triammonium citrate, 2.0 g of K2HPO4, 0.2 g of MgSO4·7H2O, 0.05 g of MnSO4·H2O, 0.1 g of tripeptide-29, and 1000.0 mL of distilled water.

[0013] As an embodiment of the present invention, the composition further comprises 10 parts of hydrolyzed Opuntia pyrifolia flower extract.

[0014] The sunscreen additive composition is used as an additive in the field of sunscreen.

[0015] As an embodiment of the present invention, the additive is added in an amount of 1wt%-5wt%.

[0016] By adopting the above technical solution, the present invention has the following beneficial effects: The composition provided by the present invention has strong antioxidant capacity, no added preservatives, and zero skin irritation; and can reach SPF60PA++++ when no more than three chemical sunscreens are used. DETAILED DESCRIPTION

[0017] The technical solution of the present invention will be described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0018] The present invention is further explained below in conjunction with specific implementation modes.

[0019] Provided is a sunscreen additive composition, comprising: roxburghii fruit extract and cactus extract.

[0020] As an embodiment of the present invention, the composition further comprises: SOD.

[0021] As an embodiment of the present invention, the composition includes 75 parts of roxburghii fruit extract, 10 parts of cactus extract, and 5 parts of SOD in terms of mass ratio.

[0022] As an embodiment of the present invention, the composition further comprises 10 parts of hydrolyzed Opuntia pyrifolia flower extract.

[0023] Rosa roxburghii fruit extract, Opuntia cactus extract In the present invention, the roxburghii fruit and the cactus are extracted together, and the specific extraction method is as follows: the roxburghii fruit and the cactus are cleaned respectively, and the surface moisture is dried at room temperature, and half of the weight of the roxburghii fruit and the whole weight of the cactus are chopped and mixed with the culture solution as soon as possible, and 15.0 g of agar is added after mixing, and the fermentation bacteria are inoculated after stirring, and fermented at 30°C for 4h, and the remaining half of the weight of the roxburghii fruit (now chopped) is added, and the fermentation is continued for 2h, and then the fermentation is stopped, and the filtrate is filtered to obtain the roxburghii fruit extract and the cactus extract.

[0024] The components of the culture medium are: 5.0 g of peptone, 3.0 g of beef extract powder, 5.0 g of NaCl, 15.0 g of oatmeal powder, 1.0 g of Tween 80, 5.0 g of sodium acetate, 2.0 g of triammonium citrate, 2.0 g of K2HPO4, 0.2 g of MgSO4·7H2O, 0.05 g of MnSO4·H2O, 0.1 g of tripeptide-29, and 1000.0 mL of distilled water.

[0025] The fermentation bacteria are Lactobacillus acidophilus and Bacillus licheniformis, and the inoculation amounts are 3wt% and 5wt% respectively.

[0026] Among them, Lactobacillus acidophilus, China Industrial Microbiological Culture Collection Management Center, CICC ® 20244.

[0027] Bacillus licheniformis, China Industrial Microbiology Culture Collection Center, CICC ® 10084.

[0028] Hydrolyzed Opuntia cactus flower extract: The preparation method of the hydrolyzed Opuntia cactus flower extract of the present invention is as follows: (1) Collect and clean the flowers of Opuntia cactus, dry them, crush them into uniform fine powder, and seal them for storage.

[0029] (2) Opuntia cactus pollen was mixed with ethanol in a ratio of 1:10 and extracted in a constant temperature water bath at 60°C and 200 rpm for 2 h.

[0030] (3) After extraction, centrifuge, filter the supernatant, and collect the filtrate as the extract.

[0031] (4) Repeat the extraction twice, combine the extracts, and concentrate to 1 / 5 of the original volume under reduced pressure to obtain a preliminary extract.

[0032] (5) The extract was added to 0.5 M HCl solution (volume ratio 1:10) and placed in a constant temperature water bath at 80 °C for 3 h for hydrolysis.

[0033] (6) After the hydrolysis is completed, add an equal volume of sodium hydroxide solution to neutralize the acidity.

[0034] (7) Remove the precipitate by centrifugation and collect the supernatant as the hydrolysate.

[0035] (8) Add cellulase (1 mg / mL) to the hydrolyzate and incubate at 37°C for 3 h with shaking.

[0036] (9) After the reaction is complete, heat to inactivate the enzyme and filter to collect the supernatant.

[0037] (10) The supernatant is concentrated by rotary evaporation until it becomes viscous or completely dry to obtain a hydrolyzed Opuntia cactus flower extract.

[0038] SOD: The SOD described in the present invention was purchased from Shenzhen Zhongke Xinyang Biotechnology Co., Ltd.

[0039] The polysaccharides of cactus are composed of macromolecular polysaccharides, which are slow to produce effects and are prone to agglomeration. The inventors found that if the polar macromolecular polysaccharides are decomposed into small molecular polysaccharides, the above phenomenon can be alleviated. At the same time, since polysaccharides exist in various forms, such as bound state and free state, many of them are often separated and purified by columns, filter membranes, etc. This step is not only cumbersome to operate, but also has limitations, and often loses a lot of effective ingredients. Therefore, the present invention uses fermentation to decompose macromolecular polysaccharides; by changing the culture medium and the culture method, macromolecular polysaccharides with smaller molecular weights are prepared. At the same time, the inventors found that although the present invention uses fermentation to obtain polysaccharides with a relatively small molecular weight range, the fermentation of cactus is slow during fermentation. The inventors found that this is because the pigments present in mature cacti have an inhibitory effect. The inventors found that the use of roxburghii can improve the above phenomenon and ferment quickly.

[0040] In addition, the inventors found in the initial research that the market demand for high-factor sunscreen products is becoming stronger and stronger, but most high-factor (SPF60 PA++++) sunscreen products rely on a large number of and a variety of chemical sunscreens and physical sunscreens to achieve the sun protection index. Most consumers apply sunscreen products on their faces for a long time. Too much chemical sunscreen and physical sunscreen will make the skin airtight and too many chemical ingredients will cause people with sensitive skin to be prone to allergies.

[0041] At present, high-factor sun protection requires reducing the content of chemical sunscreens as much as possible, but the current biological sunscreen solution has a relatively low degree of improvement in the SPF index and cannot replace chemical sunscreens to a large extent; in addition, products with high SPFs generally choose nano sunscreens when it comes to physical sunscreens, but due to their physical properties, nano sunscreens have become more and more controversial as research deepens.

[0042] However, the biological composition of the present invention is prone to discoloration, precipitation and the like due to the presence of roxburghii added to the cactus. In this regard, the inventors of the present invention have conducted in-depth research in the extraction method of roxburghii. It is found that adding tripeptide-29 and oatmeal powder to the fermented medium can improve the discoloration of roxburghii. The inventors speculate that the possible reason is that after the fermentation of tripeptide-29, when the non-enzymatic browning reaction intermediate occurs, Schiff base is formed, which can slowly inhibit the rearrangement of the intermediate and thus delay the production of stable products, i.e., colored products. It is particularly found that the technicians in this field originally used phytic acid to solve the problem of non-enzymatic browning, but the effect of phytic acid in the fermentation system is weakened. If the above-mentioned tripeptide-29 is added, the effect will not be weakened.

[0043] Embodiment 1: This embodiment provides a sunscreen additive composition, which comprises: 10 parts of cactus extract and 5 parts of SOD.

[0044] The cactus is co-extracted, and the specific extraction method is as follows: the cacti are cleaned separately, and the surface moisture is dried at room temperature, and the cacti are chopped and mixed with the culture solution as soon as possible, and 15.0 g of agar is added after mixing, and the fermentation bacteria are inoculated after stirring, and fermented at 30°C for 6h, and then the fermentation is stopped, and the filtrate is filtered to obtain the cactus extract.

[0045] The components of the culture medium are: 5.0 g of peptone, 3.0 g of beef extract powder, 5.0 g of NaCl, 15.0 g of oatmeal powder, 1.0 g of Tween 80, 5.0 g of sodium acetate, 2.0 g of triammonium citrate, 2.0 g of K2HPO4, 0.2 g of MgSO4·7H2O, 0.05 g of MnSO4·H2O, 0.1 g of tripeptide-29, and 1000.0 mL of distilled water.

[0046] The fermentation bacteria are Lactobacillus acidophilus and Bacillus licheniformis, and the inoculation amounts are 3wt% and 5wt% respectively.

[0047] Among them, Lactobacillus acidophilus, China Industrial Microbiological Culture Collection Management Center, CICC ® 20244.

[0048] Bacillus licheniformis, China Industrial Microbiology Culture Collection Center, CICC® 10084.

[0049] The SOD was purchased from Shenzhen Zhongke Xinyang Biotechnology Co., Ltd.

[0050] Example 2 This embodiment provides a sunscreen additive composition, which comprises: 75 parts of roxburghii fruit extract, 10 parts of cactus extract, and 5 parts of SOD.

[0051] The roxburghii fruit and the cactus are extracted together, and the specific extraction method is as follows: the roxburghii fruit and the cactus are cleaned separately, and the surface moisture is dried at room temperature, and half of the weight of the roxburghii fruit and the whole weight of the cactus are chopped and mixed with the culture solution as soon as possible, and 15.0 g of agar is added after mixing, and the fermentation bacteria are inoculated after stirring, and fermented at 30° C. for 4 hours, and the remaining half of the weight of the roxburghii fruit (now chopped) is added, and the fermentation is continued for 2 hours, and then the fermentation is stopped, and the filtrate is filtered to obtain the roxburghii fruit extract and the cactus extract.

[0052] The components of the culture medium are: 5.0 g of peptone, 3.0 g of beef extract powder, 5.0 g of NaCl, 15.0 g of oatmeal powder, 1.0 g of Tween 80, 5.0 g of sodium acetate, 2.0 g of triammonium citrate, 2.0 g of K2HPO4, 0.2 g of MgSO4·7H2O, 0.05 g of MnSO4·H2O, 0.1 g of tripeptide-29, and 1000.0 mL of distilled water.

[0053] The fermentation bacteria are Lactobacillus acidophilus and Bacillus licheniformis, and the inoculation amounts are 3wt% and 5wt% respectively.

[0054] Among them, Lactobacillus acidophilus, China Industrial Microbiological Culture Collection Management Center, CICC ® 20244.

[0055] Bacillus licheniformis, China Industrial Microbiology Culture Collection Center, CICC ® 10084.

[0056] The SOD was purchased from Shenzhen Zhongke Xinyang Biotechnology Co., Ltd.

[0057] Embodiment 3: The present embodiment provides a sunscreen additive composition, which comprises: 75 parts of roxburghii fruit extract, 10 parts of cactus extract, 10 parts of hydrolyzed roxburghii cactus flower extract, and 5 parts of SOD.

[0058] The roxburghii fruit and the cactus are extracted together, and the specific extraction method is as follows: the roxburghii fruit and the cactus are cleaned separately, and the surface moisture is dried at room temperature, and half of the weight of the roxburghii fruit and the whole weight of the cactus are chopped and mixed with the culture solution as soon as possible, and 15.0 g of agar is added after mixing, and the fermentation bacteria are inoculated after stirring, and fermented at 30° C. for 4 hours, and the remaining half of the weight of the roxburghii fruit (now chopped) is added, and the fermentation is continued for 2 hours, and then the fermentation is stopped, and the filtrate is filtered to obtain the roxburghii fruit extract and the cactus extract.

[0059] The components of the culture medium are: 5.0 g of peptone, 3.0 g of beef extract powder, 5.0 g of NaCl, 15.0 g of oatmeal powder, 1.0 g of Tween 80, 5.0 g of sodium acetate, 2.0 g of triammonium citrate, 2.0 g of K2HPO4, 0.2 g of MgSO4·7H2O, 0.05 g of MnSO4·H2O, 0.1 g of tripeptide-29, and 1000.0 mL of distilled water.

[0060] The fermentation bacteria are Lactobacillus acidophilus and Bacillus licheniformis, and the inoculation amounts are 3wt% and 5wt% respectively.

[0061] Among them, Lactobacillus acidophilus, China Industrial Microbiological Culture Collection Management Center, CICC ® 20244.

[0062] Bacillus licheniformis, China Industrial Microbiology Culture Collection Center, CICC ® 10084.

[0063] The preparation method of the hydrolyzed prickly pear cactus flower extract is as follows: (1) Collect and clean the flowers of Opuntia cactus, dry them, crush them into uniform fine powder, and seal them for storage.

[0064] (2) Opuntia cactus pollen was mixed with ethanol in a ratio of 1:10 and extracted in a constant temperature water bath at 60°C and 200 rpm for 2 h.

[0065] (3) After extraction, centrifuge, filter the supernatant, and collect the filtrate as the extract.

[0066] (4) Repeat the extraction twice, combine the extracts, and concentrate to 1 / 5 of the original volume under reduced pressure to obtain a preliminary extract.

[0067] (5) The extract was added to 0.5 M HCl solution (volume ratio 1:10) and placed in a constant temperature water bath at 80 °C for 3 h for hydrolysis.

[0068] (6) After the hydrolysis is completed, add an equal volume of sodium hydroxide solution to neutralize the acidity.

[0069] (7) Remove the precipitate by centrifugation and collect the supernatant as the hydrolysate.

[0070] (8) Add cellulase (1 mg / mL) to the hydrolyzate and incubate at 37°C for 3 h with shaking.

[0071] (9) After the reaction is complete, heat to inactivate the enzyme and filter to collect the supernatant.

[0072] (10) The supernatant is concentrated by rotary evaporation until it becomes viscous or completely dry to obtain a hydrolyzed Opuntia cactus flower extract.

[0073] The SOD was purchased from Shenzhen Zhongke Xinyang Biotechnology Co., Ltd.

[0074] Example 4 The present embodiment provides a sunscreen additive composition, which comprises: 75 parts of roxburghii fruit extract, 10 parts of cactus extract, 10 parts of hydrolyzed roxburghii cactus flower extract, and 5 parts of SOD.

[0075] The specific extraction method of the roxburghii fruit extract is as follows: wash the roxburghii fruit and dry the surface moisture at room temperature, chop the roxburghii fruit and mix it with the culture solution as soon as possible, add 15.0 g of agar after mixing, stir and inoculate the fermentation bacteria, ferment at 30°C for 6h, then stop the fermentation, filter and take the filtrate to obtain the roxburghii fruit extract.

[0076] The extraction method of the cactus extract is as follows: the cactus is cleaned and the surface moisture is dried at room temperature, the cactus is chopped and mixed with the culture solution as soon as possible, and 15.0 g of agar is added after mixing, and the fermentation bacteria are inoculated after stirring, and the fermentation is performed at 30° C. for 6 hours, and then the fermentation is stopped, and the filtrate is filtered to obtain the cactus extract.

[0077] The culture medium components of the two are the same, specifically: peptone 5.0 g, beef extract powder 3.0 g, NaCl 5.0 g, oatmeal powder 15.0 g, Tween 80 1.0 g, sodium acetate 5.0 g, ammonium citrate tribasic 2.0 g, K2HPO4 2.0 g, MgSO4·7H2O 0.2 g, MnSO4·H2O 0.05 g, tripeptide-29 0.1 g, and distilled water 1000.0 mL.

[0078] The fermentation bacteria are Lactobacillus acidophilus and Bacillus licheniformis, and the inoculation amounts are 3wt% and 5wt% respectively.

[0079] Among them, Lactobacillus acidophilus, China Industrial Microbiological Culture Collection Management Center, CICC ® 20244.

[0080] Bacillus licheniformis, China Industrial Microbiology Culture Collection Center, CICC ® 10084.

[0081] The preparation method of the hydrolyzed prickly pear cactus flower extract is as follows: (1) Collect and clean the flowers of Opuntia cactus, dry them, crush them into uniform fine powder, and seal them for storage.

[0082] (2) Opuntia cactus pollen was mixed with ethanol in a ratio of 1:10 and extracted in a constant temperature water bath at 60°C and 200 rpm for 2 h.

[0083] (3) After extraction, centrifuge, filter the supernatant, and collect the filtrate as the extract.

[0084] (4) Repeat the extraction twice, combine the extracts, and concentrate to 1 / 5 of the original volume under reduced pressure to obtain a preliminary extract.

[0085] (5) The extract was added to 0.5 M HCl solution (volume ratio 1:10) and placed in a constant temperature water bath at 80 °C for 3 h for hydrolysis.

[0086] (6) After the hydrolysis is completed, add an equal volume of sodium hydroxide solution to neutralize the acidity.

[0087] (7) Remove the precipitate by centrifugation and collect the supernatant as the hydrolysate.

[0088] (8) Add cellulase (1 mg / mL) to the hydrolyzate and incubate at 37°C for 3 h with shaking.

[0089] (9) After the reaction is complete, heat to inactivate the enzyme and filter to collect the supernatant.

[0090] (10) The supernatant is concentrated by rotary evaporation until it becomes viscous or completely dry to obtain a hydrolyzed Opuntia cactus flower extract.

[0091] The SOD was purchased from Shenzhen Zhongke Xinyang Biotechnology Co., Ltd.

[0092] Example 5 The present embodiment provides a sunscreen additive composition, which comprises: 75 parts of roxburghii fruit extract, 10 parts of cactus extract, 10 parts of hydrolyzed roxburghii cactus flower extract, and 5 parts of SOD.

[0093] The extraction method of the roxburghii fruit extract is as follows: the roxburghii fruit is cleaned and crushed, and then distilled water is used as the extraction solvent according to a solid-liquid ratio of 1:10, and after soaking for 2 hours, the mixture is extracted at 6 degrees Celsius for 4 hours; and the roxburghii fruit extract can be obtained by filtering.

[0094] The extraction method of the cactus extract is as follows: the cactus is cleaned and the surface moisture is dried at room temperature, the cactus is chopped and mixed with the culture solution as soon as possible, and 15.0 g of agar is added after mixing, and the fermentation bacteria are inoculated after stirring, and the fermentation is performed at 30° C. for 6 hours, and then the fermentation is stopped, and the filtrate is filtered to obtain the cactus extract.

[0095] The specific components of the culture medium are: peptone 5.0 g, beef extract powder 3.0 g, NaCl 5.0 g, oatmeal powder 15.0 g, Tween 80 1.0 g, sodium acetate 5.0 g, ammonium citrate tribasic 2.0 g, K2HPO4 2.0 g, MgSO4·7H2O 0.2 g, MnSO4·H2O 0.05 g, tripeptide-29 0.1 g, and distilled water 1000.0 mL.

[0096] The fermentation bacteria are Lactobacillus acidophilus and Bacillus licheniformis, and the inoculation amounts are 3wt% and 5wt% respectively.

[0097] Among them, Lactobacillus acidophilus, China Industrial Microbiological Culture Collection Management Center, CICC ® 20244.

[0098] Bacillus licheniformis, China Industrial Microbiology Culture Collection Center, CICC ® 10084.

[0099] The SOD was purchased from Shenzhen Zhongke Xinyang Biotechnology Co., Ltd.

[0100] Example 6 The present embodiment provides a sunscreen additive composition, which comprises: 75 parts of roxburghii fruit extract, 10 parts of cactus extract, 10 parts of hydrolyzed roxburghii cactus flower extract, and 5 parts of SOD.

[0101] The roxburghii fruit and the cactus are extracted together, and the specific extraction method is as follows: the roxburghii fruit and the cactus are cleaned separately, and the surface moisture is dried at room temperature, and half of the weight of the roxburghii fruit and the whole weight of the cactus are chopped and mixed with the culture solution as soon as possible, and 15.0 g of agar is added after mixing, and the fermentation bacteria are inoculated after stirring, and fermented at 30° C. for 4 hours, and the remaining half of the weight of the roxburghii fruit (now chopped) is added, and the fermentation is continued for 2 hours, and then the fermentation is stopped, and the filtrate is filtered to obtain the roxburghii fruit extract and the cactus extract.

[0102] The components of the culture medium are: 5.0 g of peptone, 3.0 g of beef extract powder, 5.0 g of NaCl, 15.0 g of oatmeal powder, 1.0 g of Tween 80, 5.0 g of sodium acetate, 2.0 g of triammonium citrate, 2.0 g of K2HPO4, 0.2 g of MgSO4·7H2O, 0.05 g of MnSO4·H2O, and 1000.0 mL of distilled water.

[0103] The fermentation bacteria are Lactobacillus acidophilus and Bacillus licheniformis, and the inoculation amounts are 3wt% and 5wt% respectively.

[0104] Among them, Lactobacillus acidophilus, China Industrial Microbiological Culture Collection Management Center, CICC ® 20244.

[0105] Bacillus licheniformis, China Industrial Microbiology Culture Collection Center, CICC ® 10084.

[0106] The preparation method of the hydrolyzed prickly pear cactus flower extract is as follows: (1) Collect and clean the flowers of Opuntia cactus, dry them, crush them into uniform fine powder, and seal them for storage.

[0107] (2) Opuntia cactus pollen was mixed with ethanol in a ratio of 1:10 and extracted in a constant temperature water bath at 60°C and 200 rpm for 2 h.

[0108] (3) After extraction, centrifuge, filter the supernatant, and collect the filtrate as the extract.

[0109] (4) Repeat the extraction twice, combine the extracts, and concentrate to 1 / 5 of the original volume under reduced pressure to obtain a preliminary extract.

[0110] (5) The extract was added to 0.5 M HCl solution (volume ratio 1:10) and placed in a constant temperature water bath at 80 °C for 3 h for hydrolysis.

[0111] (6) After the hydrolysis is completed, add an equal volume of sodium hydroxide solution to neutralize the acidity.

[0112] (7) Remove the precipitate by centrifugation and collect the supernatant as the hydrolysate.

[0113] (8) Add cellulase (1 mg / mL) to the hydrolyzate and incubate at 37°C for 3 h with shaking.

[0114] (9) After the reaction is complete, heat to inactivate the enzyme and filter to collect the supernatant.

[0115] (10) The supernatant is concentrated by rotary evaporation until it becomes viscous or completely dry to obtain a hydrolyzed Opuntia cactus flower extract.

[0116] The SOD was purchased from Shenzhen Zhongke Xinyang Biotechnology Co., Ltd.

[0117] Example 7 The present embodiment provides a sunscreen additive composition, which comprises: 75 parts of roxburghii fruit extract, 10 parts of cactus extract, 10 parts of hydrolyzed roxburghii cactus flower extract, and 5 parts of SOD.

[0118] The roxburghii fruit and the cactus are extracted together, and the specific extraction method is as follows: the roxburghii fruit and the cactus are cleaned separately, and the surface moisture is dried at room temperature, and half of the weight of the roxburghii fruit and the whole weight of the cactus are chopped and mixed with the culture solution as soon as possible, and 15.0 g of agar is added after mixing, and the fermentation bacteria are inoculated after stirring, and fermented at 30° C. for 4 hours, and the remaining half of the weight of the roxburghii fruit (now chopped) is added, and the fermentation is continued for 2 hours, and then the fermentation is stopped, and the filtrate is filtered to obtain the roxburghii fruit extract and the cactus extract.

[0119] The components of the culture medium are: 5.0 g of peptone, 3.0 g of beef extract powder, 5.0 g of NaCl, 1.0 g of Tween 80, 5.0 g of sodium acetate, 2.0 g of triammonium citrate, 2.0 g of K2HPO4, 0.2 g of MgSO4·7H2O, 0.05 g of MnSO4·H2O, 0.1 g of tripeptide-29, and 1000.0 mL of distilled water.

[0120] The fermentation bacteria are Lactobacillus acidophilus and Bacillus licheniformis, and the inoculation amounts are 3wt% and 5wt% respectively.

[0121] Among them, Lactobacillus acidophilus, China Industrial Microbiological Culture Collection Management Center, CICC ® 20244.

[0122] Bacillus licheniformis, China Industrial Microbiology Culture Collection Center, CICC ® 10084.

[0123] The preparation method of the hydrolyzed prickly pear cactus flower extract is as follows: (1) Collect and clean the flowers of Opuntia cactus, dry them, crush them into uniform fine powder, and seal them for storage.

[0124] (2) Opuntia cactus pollen was mixed with ethanol in a ratio of 1:10 and extracted in a constant temperature water bath at 60°C and 200 rpm for 2 h.

[0125] (3) After extraction, centrifuge, filter the supernatant, and collect the filtrate as the extract.

[0126] (4) Repeat the extraction twice, combine the extracts, and concentrate to 1 / 5 of the original volume under reduced pressure to obtain a preliminary extract.

[0127] (5) The extract was added to 0.5 M HCl solution (volume ratio 1:10) and placed in a constant temperature water bath at 80 °C for 3 h for hydrolysis.

[0128] (6) After the hydrolysis is completed, add an equal volume of sodium hydroxide solution to neutralize the acidity.

[0129] (7) Remove the precipitate by centrifugation and collect the supernatant as the hydrolysate.

[0130] (8) Add cellulase (1 mg / mL) to the hydrolyzate and incubate at 37°C for 3 h with shaking.

[0131] (9) After the reaction is complete, heat to inactivate the enzyme and filter to collect the supernatant.

[0132] (10) The supernatant is concentrated by rotary evaporation until it becomes viscous or completely dry to obtain a hydrolyzed Opuntia cactus flower extract.

[0133] The SOD was purchased from Shenzhen Zhongke Xinyang Biotechnology Co., Ltd.

[0134] Example 8 The present embodiment provides a sunscreen additive composition, which comprises: 75 parts of roxburghii fruit extract, 10 parts of cactus extract, 10 parts of hydrolyzed roxburghii cactus flower extract, and 5 parts of SOD.

[0135] The roxburghii fruit and the cactus are extracted together, and the specific extraction method is as follows: the roxburghii fruit and the cactus are cleaned separately, and the surface moisture is dried at room temperature, and half of the weight of the roxburghii fruit and the whole weight of the cactus are chopped and mixed with the culture solution as soon as possible, and 15.0 g of agar is added after mixing, and the fermentation bacteria are inoculated after stirring, and fermented at 30° C. for 4 hours, and the remaining half of the weight of the roxburghii fruit (now chopped) is added, and the fermentation is continued for 2 hours, and then the fermentation is stopped, and the filtrate is filtered to obtain the roxburghii fruit extract and the cactus extract.

[0136] The components of the culture medium are: 5.0 g of peptone, 3.0 g of beef extract powder, 5.0 g of NaCl, 15.0 g of oatmeal powder, 1.0 g of Tween 80, 5.0 g of sodium acetate, 2.0 g of triammonium citrate, 2.0 g of K2HPO4, 0.2 g of MgSO4·7H2O, 0.05 g of MnSO4·H2O, 0.1 g of tripeptide-29, and 1000.0 mL of distilled water.

[0137] The fermentation bacteria is Lactobacillus acidophilus, and the inoculation amount is 8wt%.

[0138] Among them, Lactobacillus acidophilus, China Industrial Microbiological Culture Collection Management Center, CICC ® 20244.

[0139] The preparation method of the hydrolyzed prickly pear cactus flower extract is as follows: (1) Collect and clean the flowers of Opuntia cactus, dry them, crush them into uniform fine powder, and seal them for storage.

[0140] (2) Opuntia cactus pollen was mixed with ethanol in a ratio of 1:10 and extracted in a constant temperature water bath at 60°C and 200 rpm for 2 h.

[0141] (3) After extraction, centrifuge, filter the supernatant, and collect the filtrate as the extract.

[0142] (4) Repeat the extraction twice, combine the extracts, and concentrate to 1 / 5 of the original volume under reduced pressure to obtain a preliminary extract.

[0143] (5) The extract was added to 0.5 M HCl solution (volume ratio 1:10) and placed in a constant temperature water bath at 80 °C for 3 h for hydrolysis.

[0144] (6) After the hydrolysis is completed, add an equal volume of sodium hydroxide solution to neutralize the acidity.

[0145] (7) Remove the precipitate by centrifugation and collect the supernatant as the hydrolysate.

[0146] (8) Add cellulase (1 mg / mL) to the hydrolyzate and incubate at 37°C for 3 h with shaking.

[0147] (9) After the reaction is complete, heat to inactivate the enzyme and filter to collect the supernatant.

[0148] (10) The supernatant is concentrated by rotary evaporation until it becomes viscous or completely dry to obtain a hydrolyzed Opuntia cactus flower extract.

[0149] The SOD was purchased from Shenzhen Zhongke Xinyang Biotechnology Co., Ltd.

[0150] Example 9 The present embodiment provides a sunscreen additive composition, which comprises: 75 parts of roxburghii fruit extract, 10 parts of cactus extract, 10 parts of hydrolyzed roxburghii cactus flower extract, and 5 parts of SOD.

[0151] The roxburghii fruit and cactus are extracted together, and the specific extraction method is as follows: the roxburghii fruit and the cactus are cleaned separately, and the surface moisture is dried at room temperature, half the weight of the roxburghii fruit and the whole weight of the cactus are chopped up and mixed with the culture solution as soon as possible, and fermented at 30°C for 4 hours. After adding the remaining half weight of the roxburghii fruit (chopped), the fermentation is continued for 2 hours, and then the fermentation is stopped, and the filtrate is filtered to obtain the roxburghii fruit extract and the cactus extract.

[0152] The components of the culture medium are: 5.0 g of peptone, 3.0 g of beef extract powder, 5.0 g of NaCl, 15.0 g of oatmeal powder, 1.0 g of Tween 80, 5.0 g of sodium acetate, 2.0 g of triammonium citrate, 2.0 g of K2HPO4, 0.2 g of MgSO4·7H2O, 0.05 g of MnSO4·H2O, 0.1 g of tripeptide-29, and 1000.0 mL of distilled water.

[0153] The fermentation bacteria are Lactobacillus acidophilus and Bacillus licheniformis, and the inoculation amounts are 3wt% and 5wt% respectively.

[0154] Among them, Lactobacillus acidophilus, China Industrial Microbiological Culture Collection Management Center, CICC ® 20244.

[0155] Bacillus licheniformis, China Industrial Microbiology Culture Collection Center, CICC ® 10084.

[0156] The preparation method of the hydrolyzed prickly pear cactus flower extract is as follows: (1) Collect and clean the flowers of Opuntia cactus, dry them, crush them into uniform fine powder, and seal them for storage.

[0157] (2) Opuntia cactus pollen was mixed with ethanol in a ratio of 1:10 and extracted in a constant temperature water bath at 60°C and 200 rpm for 2 h.

[0158] (3) After extraction, centrifuge, filter the supernatant, and collect the filtrate as the extract.

[0159] (4) Repeat the extraction twice, combine the extracts, and concentrate to 1 / 5 of the original volume under reduced pressure to obtain a preliminary extract.

[0160] (5) The extract was added to 0.5 M HCl solution (volume ratio 1:10) and placed in a constant temperature water bath at 80 °C for 3 h for hydrolysis.

[0161] (6) After the hydrolysis is completed, add an equal volume of sodium hydroxide solution to neutralize the acidity.

[0162] (7) Remove the precipitate by centrifugation and collect the supernatant as the hydrolysate.

[0163] (8) Add cellulase (1 mg / mL) to the hydrolyzate and incubate at 37°C for 3 h with shaking.

[0164] (9) After the reaction is complete, heat to inactivate the enzyme and filter to collect the supernatant.

[0165] (10) The supernatant is concentrated by rotary evaporation until it becomes viscous or completely dry to obtain a hydrolyzed Opuntia cactus flower extract.

[0166] The SOD was purchased from Shenzhen Zhongke Xinyang Biotechnology Co., Ltd.

[0167] Test 1: Patch test 1. Materials and Methods 1. Test substance: the original solution of the composition of Example 3.

[0168] 2. Negative control: blank control.

[0169] 3. Subjects: A total of 30 subjects, including 3 males and 27 females, aged 33 to 59 years old, with an average age of 48.10±8.13 years old, met the voluntary inclusion criteria.

[0170] 4. Patch test method: Select qualified patch test equipment and use the closed patch test method. Place about 0.020g~0.025g of the test substance in the patch tester and apply it to the back of the subject with low-allergenic tape. Remove the test substance after 24 hours and observe the skin reaction 0.5, 24, and 48 hours after removal. Record the results according to the skin reaction grading standards in the "Technical Specifications for Safety of Cosmetics" (2015 edition).

[0171] 2. Test results Table 1 Summary of cosmetics human skin patch test results

[0172] The results of human skin patch tests showed that no adverse skin reactions occurred among 30 people.

[0173] Test 2: Sunscreen test The compositions obtained in Examples 1 to 9 were added according to the formula in Table 2 to prepare a sunscreen preparation, namely Preparation 1 to Preparation 9.

[0174] Table 2 Preparation formula

[0175] SPF test method: Materials and methods 1. Test object: Preparations 3 to 9.

[0176] 2. Reference product: SPF value 16.1±2.4, prepared according to the standard formula of high SPF standard product (P2).

[0177] 3. Subjects: A total of 10 people, including 2 males and 8 females, aged 19 to 59 years old, with an average age of 37.6±13.7 years old, who met the voluntary inclusion criteria.

[0178] 4. Light source: Daylight simulator xenon arc lamp, all performance indicators meet the measurement specification requirements.

[0179] 5. Test method: Test in accordance with the specific requirements of the currently valid technical specifications. The subject takes a forward leaning position and the back is irradiated. The minimum erythema dose (MED value) of the subject's skin to ultraviolet radiation is predicted 24 hours before the test, and the ultraviolet radiation dose is adjusted according to the predicted results for testing the object. On the day of the test, a spot no less than 30cm on the subject's back is first selected. 2 Normal skin area, (2.00 ± 0.05) mg / cm 2 Apply the test object or reference product evenly to the above area, and then select the irradiation dose according to the specification requirements, and irradiate in three situations: ① The test object is not applied to the subject's skin; ② The reference product is applied; ③ The test object is applied. Observe the experimental results after 24 hours and record the MED values ​​of the three situations respectively. 6. SPF value calculation method: The SPF value of the test object or reference product protecting a single subject is expressed by the following formula: SPFi=(MED value of protected skin) / (MED value of unprotected skin).

[0180] Table 3 Test results of test 2

[0181] Test 3: Place preparations 1 to 9 at 5°C, 25°C, and 40°C for 90 days, leave them uncovered, and observe changes in properties. In addition, conduct a hot and cold cycle experiment, place them at 5°C and 40°C for 12 hours each, repeat this cycle three times, and observe changes in properties.

[0182] Table 4 Test results of test 3

[0183] Test 4: Hyaluronidase inhibition rate The test is based on the HMC-W1-029 hyaluronidase inhibition rate.

[0184] Preparation 3-9: Dilute with pure water to a sample concentration of 0.2%; Positive control (dipotassium glycyrrhizinate, purity ≥98%): Dilute with water to a positive control concentration of 3%; Negative control: pure water.

[0185] Test operation steps: Design the embodiment group, positive control group and negative control group, and set up 3 parallels for each group. Add different reagent solutions respectively, shake well, and place at room temperature for 30 minutes for color development. Use ultraviolet spectrophotometer to measure the absorbance value at a wavelength of 528nm.

[0186] Calculation formula Hyaluronidase inhibition rate (%) = (1-(CD) / (AB)) × 100 Wherein, A is the absorbance of the reaction solution without sample; B is the absorbance of the reaction solution without sample and enzyme; C is the absorbance of the reaction solution containing sample and enzyme; D is the absorbance of the reaction solution containing sample and no enzyme.

[0187] The statistical analysis software was SPSS, and the comparison of hyaluronidase inhibition rates among the test samples, positive controls, and negative controls was performed using an independent sample t-test. The above statistical analyses were all two-tailed tests, with a significance level of α = 0.05, P > 0.05, indicating no significant difference between the two groups; P < 0.05, indicating a significant difference between the two groups.

[0188] Table 1 Test results

[0189] The hyaluronidase inhibition rate was rounded to three decimal places, and P < 0.05 indicated that there were significant differences between the sample and the positive control and the negative control.

[0190] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A sunscreen additive composition, characterized in that: The composition comprises: roxburghii fruit extract and cactus extract.

2. The sunscreen additive composition according to claim 1, characterized in that: The composition further comprises: SOD.

3. The sunscreen additive composition according to claim 2, characterized in that: According to the mass ratio, the composition comprises 75 parts of roxburghii fruit extract, 10 parts of cactus extract and 5 parts of SOD.

4. The sunscreen additive composition according to any one of claims 1 to 3, characterized in that: The roxburghii fruit extract is prepared by fermenting the roxburghii fruit.

5. The sunscreen additive composition according to any one of claims 1 to 3, characterized in that: The cactus extract is prepared by fermenting cactus.

6. The sunscreen additive composition according to claim 4, characterized in that The bacterial species for fermenting the roxburghii fruit are: Lactobacillus acidophilus and Bacillus licheniformis.

7. The sunscreen additive composition according to claim 5, characterized in that The culture medium for fermenting cactus comprises: 5.0 g of peptone, 3.0 g of beef extract powder, 5.0 g of NaCl, 15.0 g of oatmeal powder, 1.0 g of Tween 80, 5.0 g of sodium acetate, 2.0 g of triammonium citrate, 2.0 g of K2HPO4, 0.2 g of MgSO4·7H2O, 0.05 g of MnSO4·H2O, 0.1 g of tripeptide-29, and 1000.0 mL of distilled water.

8. The sunscreen additive composition according to claim 3, characterized in that The composition also includes 10 parts of hydrolyzed Opuntia pyrifolia flower extract.

9. The sunscreen additive composition according to any one of claims 1 to 8, characterized in that: It is used as an additive in the field of sun protection.

10. The use according to claim 9, characterized in that: The additive is added in an amount of 1 wt% to 5 wt%.

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