Mass production method of mushroom extract
By optimizing the extraction process of mushroom extract and using two heating and microwave drying technology, the problems of high mass production cost, large energy consumption and low extraction rate of mushroom extract are solved, and efficient and low-cost mushroom extract production are achieved. Improved product quality.
Patent Information
- Application Number
- CN202411497073.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-10-25
- Publication Date
- 2024-12-27
AI Technical Summary
The existing mass production methods of mushroom extracts are high cost, high energy consumption, low extraction rate and insufficient crude polysaccharide content, which affects product quality.
After crushing the dried shiitake mushroom rod, the extraction was performed twice in a steam-heated extraction tank. Combined with microwave drying technology, the water content is controlled at 5-7%, and the water addition and cooking time are optimized to ensure efficient extraction of shiitake mushroom extract.
It significantly improves the yield and crude polysaccharide content of mushroom extract, shortens production time, reduces energy consumption and cost, improves product quality, and is suitable for industrial mass production.
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Figure CN119185381A_ABST
Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of traditional Chinese medicine extraction, and particularly relates to a mass production method of shiitake mushroom extract. Background Art
[0002] Shiitake mushrooms are not only a delicious food for ordinary people, but also have many beneficial effects and functions for the human body. The further processed shiitake mushroom extract is mainly used as one of the raw material formulas of traditional Chinese medicine health food. Relevant data prove that after taking it, it can improve the body's immune function, prevent cancer, lower blood pressure, lower blood lipids, lower cholesterol, promote calcium absorption and lose weight. It also has a therapeutic effect on diabetes, tuberculosis, infectious hepatitis, neuritis, etc. It can also be used for indigestion, constipation, weight loss, etc.
[0003] The conventional method of extracting shiitake mushroom extract is boiling. In actual mass production, there are many problems in details such as the amount of water added, cooking time, number of cooking times, and drying methods, which leads to high mass production costs for most mass production companies, large energy losses, low crude polysaccharide content in the extract, and low yield, which greatly reduces the quality of shiitake mushroom extract.
[0004] Therefore, there is an urgent need for a mass production method of shiitake mushroom extract that is suitable for industrial mass production and low cost. Summary of the invention
[0005] The purpose of the present invention is to overcome the above-mentioned shortcomings and provide a method for mass production of shiitake mushroom extracts. The method can improve the yield of shiitake mushroom extracts and the content of crude polysaccharides of shiitake mushrooms while mass producing shiitake mushroom extracts, shorten the time of actual mass production of shiitake mushroom extracts, and significantly reduce energy consumption. The method of the present invention can improve product quality on the basis of greatly reducing mass production costs.
[0006] The object of the present invention is achieved in the following ways:
[0007] A method for mass production of shiitake mushroom extract, the method comprising the following steps:
[0008] Take dried shiitake mushroom sticks, crush them through a 30-60 mesh sieve, and then put them into a steam-heated extraction tank, add water 20-30 times the weight of the shiitake mushroom sticks, heat to 100°C, turn off the steam heating, keep boiling and stand overnight; heat and keep boiling for 3-4 hours on the second day, extract the extract into a concentration tank; add water 20-30 times the weight of the shiitake mushroom sticks into the extraction tank for the second time, heat to 100°C and keep boiling for 3-4 hours, and extract the extract into a concentration tank; mix and concentrate the two shiitake mushroom extracts into a fluid extract, and dry until the water content is controlled to 5-7%, preferably 6%.
[0009] Preferably, the shiitake mushroom sticks are crushed and then passed through a 30-mesh sieve.
[0010] Preferably, 20 times the weight of water of the shiitake mushroom sticks is added each time.
[0011] Preferably, the boiling time is 3 hours each time.
[0012] Preferably, the drying is microwave drying.
[0013] Preferably, the microwave drying is to spread the fluid extract flat on a plate with a thickness of 1-1.5 cm, first use low power 3KW to dry for 1 hour, then use high power 5KW to dry for 2 hours, and finally use low power 3KW to dry for 1 hour, the total time is 4 hours, and the material is turned over every 1 hour.
[0014] Through a large number of tests, the present invention has found that under the optimal process conditions for mass production of shiitake mushroom extract, the yield of shiitake mushroom extract is about 31%, and the shiitake mushroom extract contains about 11% crude polysaccharide. The water content of the material is relatively uniform, at 5-7%, and there is no burning phenomenon. The present invention ensures the quality of mass-produced shiitake mushroom extract under the conditions of lower power consumption, reduced resource waste, reduced sewage, etc.
[0015] Compared with the prior art, the present invention has the following beneficial effects: the shiitake mushroom extract processed by the method of the present invention has a high extraction rate and effectively maintains the effective ingredients in the product, saves energy and reduces consumption, reduces sewage, greatly reduces mass production costs, and is particularly suitable for industrial batch production. BRIEF DESCRIPTION OF THE DRAWINGS
[0016] Figure 1 This is a photo of the shiitake mushroom extract after microwave drying and crushing in Experimental Example 12.
[0017] Figure 2 This is a photo of the shiitake mushroom extract after being dried and crushed in a hot air circulation oven in Test Example 13.
[0018] Figure 3 This is a photograph of the spray drying failure of Test Example 14. DETAILED DESCRIPTION
[0019] The technical solution of the present invention is further described in detail below in conjunction with several preferred embodiments and drawings. This embodiment is implemented on the premise of the technical solution of the invention, and a detailed implementation method and specific operation process are given, but the protection scope of the present invention is not limited to the following embodiments.
[0020] The experimental methods described in the following examples are conventional methods unless otherwise specified, and are performed according to the techniques or conditions described in the literature in the field or according to the product instructions. The materials, reagents, etc. described in the following examples are all commercially available unless otherwise specified.
[0021] The equipment used in the following embodiments and test examples is a 200L multifunctional extraction and concentration mass production line, a HWZ-30B box-type microwave vacuum drying equipment, a ZPG-50 centrifugal sprayer, and a TG-ZAI hot air circulation oven.
[0022] Example 1
[0023] Take dried shiitake mushroom sticks, crush them through a 30-mesh sieve to get 5.00 kg, put them into a 200L extraction tank heated by steam, add water 20 times the weight of the shiitake mushroom sticks, heat to 100°C, turn off the steam heating, keep boiling until the end and let it stand overnight; heat and keep boiling for 3 hours on the second day, extract the liquid into the concentration tank; add 100 kg of water 20 times the weight of the shiitake mushroom sticks to the extraction tank for the second time, heat to 100°C and keep boiling for 3.0 hours, and also extract the liquid into the concentration tank. The two shiitake mushroom extracts are mixed in series and concentrated into a fluid extract, and then microwave drying is performed. The microwave drying is to spread the fluid extract on a plate with a thickness of 1-1.5 cm, first use low power 3KW to dry for 1 hour, then use high power 5KW to dry for 2 hours, and finally use low power 3KW to dry for 1 hour, the total time is 4 hours, and the material is turned over every 1 hour. After the water content was controlled to 6%, 1.55 kg of shiitake mushroom extract was obtained, and the extract yield was 31.00%. The shiitake mushroom residue contained 0% crude polysaccharide, and the shiitake mushroom extract contained 11.2% crude polysaccharide.
[0024] In the following test examples, the main influencing factors of shiitake mushroom extraction were tested, and only the process data with obvious differences were listed for comparison. Finally, a stable, economical and environmentally friendly mass production process of shiitake mushroom extract was explored, providing a reference for improving mass production capacity and efficiency and reducing mass production costs.
[0025] Test Example 1
[0026] Raw material pretreatment: Fresh whole shiitake mushroom fruiting bodies were dried and then crushed through a 30-mesh sieve to obtain shiitake mushroom crumbs No. 1, and the crude polysaccharide content was measured to be 5.1%.
[0027] Take 5.00kg of No. 1 shiitake mushroom minced meat and put it into a 200L extraction tank, and add 15 times the weight of water, heat it to 100℃, then turn off the steam heating, keep boiling until the end and let it stand overnight; on the second day, heat and keep boiling for 3h, extract the medicinal liquid into the concentration tank; for the second time, add 15 times the amount of water, i.e. 75kg, to the extraction tank, heat it to 100℃ and keep boiling for 3.0h, and also extract the medicinal liquid into the concentration tank.
[0028] The secondary shiitake extracts were mixed and concentrated in series and then microwave-dried. After the water content was controlled to 6%, 1.28 kg of shiitake extract was obtained, with an extract yield of 25.60%. The shiitake residues contained 0.20% crude polysaccharides, and the shiitake extract contained 10.50% crude polysaccharides.
[0029] Test Example 2
[0030] Take 5.00 kg of No. 1 shiitake mushroom minced meat pretreated in Experimental Example 1 and put it into a 200L extraction tank, add 20 times the weight of water, heat it to 100°C, turn off the steam heating, keep boiling until the end and let it stand overnight; on the second day, heat and keep boiling for 3 hours, extract the medicinal liquid into the concentration tank; for the second time, add 20 times the amount of water, i.e. 100 kg, to the extraction tank, heat it to 100°C and keep boiling for 3.0 hours, and also extract the medicinal liquid into the concentration tank.
[0031] The two shiitake mushroom extracts were mixed and concentrated in series to obtain a fluid extract, which was then microwave-dried and the water content was controlled to 6% to obtain 1.52 kg of shiitake mushroom extract with an extract yield of 30.40%. The shiitake mushroom residues contained 0% crude polysaccharides, and the shiitake mushroom extract contained 11.00% crude polysaccharides.
[0032] Test Example 3
[0033] Take 5.00 kg of No. 1 shiitake mushroom minced meat pretreated in Experimental Example 1 and put it into a 200L extraction tank, add 30 times the weight of water, heat to 100°C and then turn off the steam heating, keep boiling until the end and let it stand overnight; on the second day, heat and keep boiling for 3 hours, extract the medicinal liquid into the concentration tank; for the second time, add 30 times the amount of water, i.e. 150 kg, to the extraction tank, heat to 100°C and keep boiling for 3.0 hours, and also extract the medicinal liquid into the concentration tank.
[0034] The two shiitake mushroom extracts were mixed and concentrated in series to obtain a fluid extract, which was then microwave-dried and the water content was controlled to 6% to obtain 1.57 kg of shiitake mushroom extract with an extract yield of 31.40%. The shiitake mushroom residues contained 0% crude polysaccharides, and the shiitake mushroom extract contained 11.52% crude polysaccharides.
[0035] With other experimental conditions unchanged, the optimal amount of water added was found in the typical data test examples 1, 2, and 3. Considering the cost control and environmental protection issues of actual industrial mass production, the amount of water added for mushroom extraction was 20 times the weight.
[0036] Test Example 4
[0037] Take 5.00 kg of No. 1 shiitake mushroom minced meat pretreated in Experimental Example 1 and put it into a 200L extraction tank, add 20 times the weight of water, heat to 100°C, turn off the steam heating, keep boiling until the end and let it stand overnight; on the second day, heat and keep boiling for 2 hours, extract the medicinal liquid into the concentration tank; for the second time, add 20 times the amount of water, i.e. 100 kg, to the extraction tank, heat to 100°C and keep boiling for 2.0 hours, and also extract the medicinal liquid into the concentration tank.
[0038] The two shiitake mushroom extracts were mixed and concentrated in series to obtain a fluid extract, which was then microwave-dried and the water content was controlled to 6% to obtain 1.30 kg of shiitake mushroom extract with an extract yield of 26.00%. The shiitake mushroom residues contained 0.11% crude polysaccharides, and the shiitake mushroom extract contained 10.82% crude polysaccharides.
[0039] Test Example 5
[0040] Take 5.00 kg of No. 1 shiitake mushroom minced meat pretreated in Experimental Example 1 and put it into a 200L extraction tank, add 20 times the weight of water, heat it to 100°C, turn off the steam heating, keep boiling until the end and let it stand overnight; on the second day, heat and keep boiling for 4 hours, extract the medicinal liquid into the concentration tank; for the second time, add 20 times the amount of water, i.e. 100 kg, to the extraction tank, heat it to 100°C and keep boiling for 4.0 hours, and also extract the medicinal liquid into the concentration tank.
[0041] The two shiitake mushroom extracts were mixed and concentrated in series to obtain a fluid extract, which was then microwave-dried and the water content was controlled to 6% to obtain 1.55 kg of shiitake mushroom extract with an extract yield of 31.00%. The shiitake mushroom residues contained 0% crude polysaccharides, and the shiitake mushroom extract contained 11.53% crude polysaccharides.
[0042] Through the optimal amount of water added and other conditions unchanged, the typical data of test examples 2, 4, and 5 show that short boiling time cannot fully release lentinan, and long boiling time wastes energy and has no obvious effect. The best boiling time for shiitake mushroom extraction is 3 hours each time.
[0043] Test Example 6
[0044] Take 5.00 kg of No. 1 shiitake mushroom minced meat pretreated in Experiment 1 and put it into a 200L extraction tank, add 20 times the weight of water, heat to 100°C and then turn off the steam heating, keep boiling until the end and let it stand overnight; on the next day, heat and keep boiling for 3 hours, and extract the liquid into the concentration tank.
[0045] The shiitake extracts were mixed and concentrated in series to obtain a fluid extract, which was then microwave dried. After the water content was controlled to 6%, 1.01 kg of shiitake extract was obtained, with an extract yield of 20.20%. The shiitake residues contained 0.53% crude polysaccharides, and the shiitake extract contained 8.5% crude polysaccharides.
[0046] Test Example 7
[0047] Take 5.00 kg of No. 1 shiitake mushroom minced meat pretreated in Test Example 1 and put it into a 200L extraction tank, add 20 times the weight of water, heat to 100°C, turn off the steam heating, keep boiling until the end and let it stand overnight; heat and keep boiling for 3 hours on the second day, extract the medicinal liquid into the concentration tank; add 20 times the amount of water, i.e. 100 kg, to the extraction tank for the second time, heat to 100°C and keep boiling for 3.0 hours, and extract the medicinal liquid into the concentration tank in the same way; add 20 times the amount of water, i.e. 100 kg, to the extraction tank for the third time, heat to 100°C and keep boiling for 3.0 hours, and extract the medicinal liquid into the concentration tank in the same way (the color of the medicinal liquid is obviously clear the third time).
[0048] The three shiitake mushroom extracts were mixed and concentrated in series to obtain a fluid extract, which was then microwave-dried. After the water content was controlled to 6%, 1.62 kg of shiitake mushroom extract was obtained, with an extract yield of 32.40%. The shiitake mushroom residues contained 0% crude polysaccharides, and the shiitake mushroom extract contained 11.84% crude polysaccharides.
[0049] From the typical data of test examples 2, 6, and 7, it can be seen that the yield is low and the crude polysaccharide content of shiitake mushrooms is also low if the number of boiling and filtering is small. Excessive boiling and filtering wastes energy and has no obvious effect. Therefore, the best number of boiling times for shiitake mushroom extraction is twice.
[0050] Test Example 8
[0051] Take the dried shiitake mushroom fruiting bodies of the same batch in Test Example 1, directly take 5.00 kg and put them into a 200L extraction tank, add 20 times the weight of water, heat to 100°C, turn off the steam heating, keep boiling until the end and let it stand overnight; on the second day, heat and keep boiling for 3 hours, extract the drug liquid into the concentration tank; for the second time, add 20 times the amount of water, i.e. 100 kg, to the extraction tank, heat to 100°C and keep boiling for 3.0 hours, and also extract the drug liquid into the concentration tank.
[0052] The two shiitake mushroom extracts were mixed and concentrated in series to obtain a fluid extract, which was then microwave-dried and the water content was controlled to 6% to obtain 1.08 kg of shiitake mushroom extract with an extract yield of 21.60%. The shiitake mushroom residues contained 0.56% crude polysaccharides, and the shiitake mushroom extract contained 10.06% crude polysaccharides.
[0053] Test Example 9
[0054] Take the dried shiitake mushroom fruiting bodies from the same batch in Test Example 1, crush them through a 60-mesh sieve, take 5.00 kg and put them into a 200L extraction tank, add 20 times the weight of water, heat to 100°C, turn off the steam heating, keep boiling until the end and let it stand overnight; on the second day, heat and keep boiling for 3 hours, extract the liquid into the concentration tank; for the second time, add 20 times the amount of water, i.e. 100 kg, to the extraction tank, heat to 100°C and keep boiling for 3.0 hours, and also extract the liquid into the concentration tank.
[0055] The fluid extract obtained by mixing and concentrating the two shiitake mushroom extracts in series was microwave-dried, and the water content was controlled to 6% to obtain 1.55 kg of shiitake mushroom extract, with an extract yield of 31.00%. The shiitake mushroom residue contained 0% crude polysaccharides, and the shiitake mushroom extract contained 11.26% crude polysaccharides.
[0056] The typical data of test examples 2, 8, and 9 show that the yield of direct steaming of shiitake mushrooms without crushing is low and the crude polysaccharide content of shiitake mushrooms is also low. If the crushing mesh is too fine, processing time and energy will be wasted, and there will be no obvious effect. Therefore, the best crushing mesh of shiitake mushrooms is to pass through a 30-mesh sieve.
[0057] Test Example 10
[0058] Since the price of complete shiitake fruiting bodies in the market is too high, and the incomplete fruiting bodies such as shredded shiitake caps and shiitake sticks are not good-looking, the price is low in the market. The average price of shredded shiitake caps or shiitake sticks is about half of the good-looking, complete shiitake fruiting bodies. Therefore, the fresh and complete shiitake fruiting bodies were separated from the shiitake caps and shiitake sticks, and each was crushed and tested. It was found that the shiitake caps contained 4.8% crude polysaccharides, while the shiitake sticks contained 5.5% crude polysaccharides. The crude polysaccharide content in the sticks was significantly higher than that in the caps.
[0059] Take the mushroom caps, crush them through a 30-mesh sieve to get 5.00 kg, put them into a 200L extraction tank, and add 20 times the weight of water. Heat to 100°C and then turn off the steam heating. Keep boiling until the end and let it stand overnight. Heat and keep boiling for 3 hours on the second day, extract the liquid into the concentration tank. For the second time, add 20 times the amount of water, i.e. 100 kg, to the extraction tank, heat to 100°C and keep boiling for 3.0 hours, and extract the liquid into the concentration tank in the same way.
[0060] The two shiitake mushroom extracts were mixed and concentrated in series to obtain a fluid extract, which was then microwave-dried. After the water content was controlled to 6%, 0.96 kg of shiitake mushroom extract was obtained, with an extract yield of 19.20%. The shiitake mushroom residues contained 0% crude polysaccharides, and the shiitake mushroom extract contained 9.32% crude polysaccharides.
[0061] Test Example 11
[0062] Take shiitake mushroom sticks, crush them through a 30-mesh sieve to get 5.00 kg, put them into a 200L extraction tank heated by steam, add water 20 times the weight of the shiitake mushroom sticks, heat to 100°C and then turn off the steam heating, keep boiling until the end and let it stand overnight; on the second day, heat and keep boiling for 3 hours, extract the medicinal liquid into a concentration tank; for the second time, add 20 times the amount of water, i.e. 100 kg, to the extraction tank, heat to 100°C and keep boiling for 3.0 hours, and similarly extract the medicinal liquid into the concentration tank.
[0063] The two shiitake mushroom extracts were mixed and concentrated in series to obtain a fluid extract, which was then microwave-dried. After the water content was controlled to 6%, 1.55 kg of shiitake mushroom extract was obtained, with an extract yield of 31.00%. The shiitake mushroom residues contained 0% crude polysaccharides, and the shiitake mushroom extract contained 11.2% crude polysaccharides.
[0064] The typical data test examples 2, 10, and 11 show that the yield and crude polysaccharide content of the three types of complete shiitake mushroom fruiting bodies, shiitake mushroom caps, and shiitake mushroom sticks in the market can meet expectations, and greatly reduce the comprehensive mass production cost of the product. Therefore, when purchasing raw materials, it is best to purchase clean shiitake mushroom sticks.
[0065] The microwave drying in the above test example is to spread the fluid extract flat on a plate with a thickness of 1-1.5 cm, first use low power 3KW to dry for 1 hour, then use high power 5KW to dry for 2 hours, and finally use low power 3KW to dry for 1 hour, the total time is 4 hours, and the material is turned over every 1 hour.
[0066] Test Example 12
[0067] A microwave drying process for shiitake mushroom extracts comprises the following steps:
[0068] The shiitake mushroom extract liquid extract obtained by the method of Test Example 11 was spread on a plate with a thickness of 1-1.5 cm. It was first dried at low power of 3KW for 1 hour, then at high power of 5KW for 2 hours, and finally at low power of 3KW for 1 hour. The total time was 4 hours, and the material was turned over every 1 hour.
[0069] Product properties: The crude polysaccharide content of shiitake mushroom extract is about 11.0%, and the average water content is about 6%, which meets the material standards, such as Figure 1 .
[0070] Test Example 13
[0071] A process for hot air circulation drying of shiitake mushroom extract comprises the following steps:
[0072] The shiitake mushroom extract liquid extract obtained by the method of Test Example 11 (same weight as that of Test Example 12) was spread on a plate with a thickness of 1-1.5 cm. It was first dried with hot air at 70 degrees for 48 hours, and the material was turned over several times when appropriate.
[0073] Product properties: The crude polysaccharide content of shiitake mushroom extract is about 11.0%, and the average water content is about 6%, which meets the material standards, such as Figure 2 However, the drying time is too long, the energy consumption is too high, and the mass production efficiency is too low.
[0074] Test Example 14
[0075] A process for spray drying of shiitake mushroom extract comprises the following steps:
[0076] The shiitake mushroom extract liquid extract obtained by the method of Test Example 11 (same weight as that of Test Example 12) was transported to the spray drying feeding room and added into the feeding tank and kept warm at 60°C.
[0077] First, turn on the air supply fan, then turn on the exhaust fan and the heating device, and check for leaks: If normal, the system can be preheated. When the air supply temperature reaches about 200°C and the air outlet temperature is about 110°C, the preheating is complete. When the inlet temperature and outlet temperature of the drying chamber reach the set temperature, turn on the centrifugal sprayer, turn on the feed pump, and start atomization heating. Pay attention to the inlet and outlet temperatures during the drying process. After a few minutes, the viscous liquid blocked the spray gun nozzle of the spray tower, and the experiment failed. Figure 3 Agglomeration.
[0078] All the above experiments were performed using a 200L multifunctional extraction and concentration mass production line.
[0079] The above crude polysaccharide content is determined in accordance with the polysaccharide determination method in Appendix B (Normative Appendix) of Q / NJZK 0007S-2020.
[0080] Polysaccharide determination method
[0081] B.1 Principle
[0082] Use hot water to extract total sugars, and use high-concentration ethanol to precipitate polysaccharides; sugars react with sulfuric acid and dehydrate to generate hydroxymethyl furan formaldehyde (hydroxymethyl sugar alcohol), which then condenses with anthrone to form a blue compound, the color depth of which is proportional to the concentration of sugar in the solution. Quantitative determination can be performed using a spectrophotometer, and the absorption wavelength is 625nm.
[0083] B.2 Instruments and reagents
[0084] Spectrophotometer, electric constant temperature water bath, analytical balance, centrifuge, glucose (AR), anthrone (AR), concentrated sulfuric acid (AR), ethanol (AR). Anthrone reagent (0.1 g anthrone dissolved in 100 ml of 80% concentrated sulfuric acid).
[0085] B.3 Sample processing
[0086] Take 1g of the sample contents under the item of filling volume difference, weigh accurately, add 90ml of hot water, extract in a boiling water bath for 2h, dilute to 100ml volumetric flask, filter, accurately measure 2ml, add 30ml of ethanol, shake well, place at 4℃ for 12h, take out and centrifuge, pour off the supernatant, dissolve the precipitate in water, shake well, dilute to 100ml as the test solution.
[0087] B.4 Preparation of standard curve
[0088] Prepare 100 mg / L glucose solution, accurately measure 0.2 ml, 0.4 ml, 0.6 ml, 0.8 ml, 1.0 ml, 1.2 ml of the reference solution, respectively place them in 10 ml volumetric flasks, add water to 2.0 ml, then accurately add 6 ml of anthrone sulfate solution, shake well, heat in a boiling water bath for 15 minutes, take out, shake well, cool in ice water for 15 minutes, use the corresponding reagent as blank, and test according to the UV-visible spectrophotometry, measure the absorbance at a wavelength of 625 nm, and draw a standard curve with absorbance as the ordinate and concentration as the abscissa.
[0089] B.5 Determination of polysaccharides in samples
[0090] Accurately measure 2 ml of the test solution and place it in a 10 ml stoppered test tube. According to the method under the standard curve preparation, starting from "add 6 ml of anthrone sulfate solution", determine the absorbance according to the law, read the amount of glucose in the test solution from the standard curve, and calculate the result.
[0091] B.6 Calculation
[0092] Calculation formula:
[0093]
[0094] Where:
[0095] X---------polysaccharide content in the sample, g / 100g;
[0096] m1--------the mass of glucose in the sample solution, mg; m--------the mass of the sample, mg;
[0097] V1--------Sample processing volume, ml;
[0098] V2--------volume of sample solution used for precipitating polysaccharide, ml; V3--------volume of precipitated polysaccharide, ml;
[0099] V4--------Volume of sample solution used for measurement, ml.
Claims
1. A method for mass production of shiitake mushroom extract, characterized in that The method comprises the following steps: Take dried shiitake mushroom sticks, crush them through a 30-60 mesh sieve, and then put them into a steam-heated extraction tank, add water 20-30 times the weight of the shiitake mushroom sticks, heat to 100°C, turn off the steam heating, keep boiling until the end and let it stand overnight; heat and keep boiling for 3-4 hours on the second day, extract the extract into a concentration tank; add water 20-30 times the weight of the shiitake mushroom sticks into the extraction tank for the second time, heat to 100°C and keep boiling for 3-4 hours, and extract the extract into a concentration tank; the two shiitake mushroom extracts are mixed and concentrated into a fluid extract, and dried until the water content is controlled to 5-7%.
2. The mass production method of shiitake mushroom extract according to claim 1, characterized in that The mushroom sticks are crushed and then passed through a 30-mesh sieve.
3. The mass production method of shiitake mushroom extract according to claim 1, characterized in that The water of 20 times the weight of the mushroom sticks is added each time.
4. The method for mass production of shiitake mushroom extract according to claim 1, characterized in that The boiling is maintained for 3 hours each time.
5. The method for mass production of shiitake mushroom extract according to claim 1, characterized in that The drying is microwave drying.
6. The method for mass production of shiitake mushroom extract according to claim 5, characterized in that The microwave drying is to spread the fluid extract flat on a plate with a thickness of 1-1.5 cm, first use low power 3KW to dry for 1 hour, then use high power 5KW to dry for 2 hours, and finally use low power 3KW to dry for 1 hour, the total time is 4 hours, and the material is turned over every 1 hour.
7. The method for mass production of shiitake mushroom extract according to claim 1, characterized in that The drying is performed until the moisture content is controlled to 6%.