Veterinary traditional Chinese medicine oral liquid for preventing and treating proventriculitis of poultry and application thereof

Abstract

The invention relates to the technical field of traditional Chinese medicine preparations, and provides veterinary traditional Chinese medicine oral liquid for preventing and treating poultry glandular and muscular gastritis and application of the veterinary traditional Chinese medicine oral liquid for preventing and treating the poultry glandular and muscular gastritis. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 30 to 50 parts of rhizoma coptidis, 20 to 30 parts of cortex phellodendri, 40 to 50 parts of herba taraxaci, 20 to 30 parts of herba patriniae, 30 to 40 parts of radix astragali seu hedysari, 80 to 100 parts of cortex fraxini, 20 to 40 parts of rhizoma atractylodis, 50 to 70 parts of poria cocos, 10 to 30 parts of rhizoma alismatis, 20 to 40 parts of pericarpium citri reticulatae, 10 to 30 parts of fried medicated leaven, 20 to 30 parts of fried fructus crataegi, 10 to 30 parts of fried malt, 10 to 30 parts of radix angelicae sinensis, 20 to 30 parts of rhizoma cyperi, 10 to 30 parts of rhizoma curcumae and 10 to 20 parts of radix glycyrrhizae preparata. According to the technical scheme, the problems of reduced feed intake, slow growth and development, emaciation and the like of poultry caused by glandular ventriculitis in the prior art are solved.

Description

Technical Field

[0001] The invention relates to the technical field of Chinese medicine preparations, and in particular to an veterinary Chinese medicine oral liquid for preventing and treating poultry adenomyotic gastritis and an application thereof. Background Art

[0002] Poultry adenomyotic gastritis is a poultry digestive system disease. The main manifestations are enlarged glandular stomach, flat or even disappeared glandular stomach papillae, thickened, eroded, and ulcerated stratum corneum of the muscular stomach. In the process of poultry farming, adenomyotic gastritis is a common disease, which has brought serious troubles to the poultry farming industry. When poultry suffer from adenomyotic gastritis, the digestive system of poultry will be damaged. The glandular stomach and muscular stomach mucosa of the diseased poultry will be damaged, which will affect the secretion of digestive enzymes and the digestion and absorption of food, resulting in a decrease in feed conversion rate. A large amount of inadequately digested feed is excreted from the body, resulting in a waste of resources. At the same time, indigestion may also cause intestinal diseases such as diarrhea and enteritis, further damaging the health of poultry. This not only directly affects the growth rate and weight gain of poultry, but also prolongs the breeding cycle and increases breeding costs.

[0003] As a traditional medical resource in my country, Chinese medicine has fewer side effects than chemical drugs and will not cause serious damage to the liver, kidneys and other organs of poultry. At the same time, Chinese medicine is not easy to develop drug resistance and can effectively prevent and treat diseases in the long term, which is of great significance to ensure the health of poultry and food safety. Based on this, it is of great practical significance to develop a veterinary Chinese medicine oral liquid for the prevention and treatment of poultry adenomyosis gastritis. Summary of the invention

[0004] The present invention provides a veterinary Chinese medicine oral liquid for preventing and treating poultry adenomyosis gastritis and application thereof, which has the effects of clearing away heat and dampness, strengthening the spleen and stomach, and solves the problems of reduced feed intake, slow growth and development, and emaciation of poultry caused by adenomyosis gastritis in the related art.

[0005] The technical scheme of the present invention is as follows: The present invention proposes an veterinary Chinese medicine oral liquid for preventing and treating poultry glandular gastritis, wherein the raw materials include the following components in parts by weight: 30-50 parts of coptis chinensis, 20-30 parts of phellodendron, 40-50 parts of dandelion, 20-30 parts of patrinia herb, 30-40 parts of astragalus, 80-100 parts of qinpi, 20-40 parts of atractylodes, 50-70 parts of tuckahoe, 10-30 parts of orientalis, 20-40 parts of tangerine peel, 10-30 parts of stir-fried Shenqu, 20-30 parts of stir-fried hawthorn, 10-30 parts of stir-fried malt, 10-30 parts of angelica, 20-30 parts of cyperus rotundus, 10-30 parts of curcuma, and 10-20 parts of roasted liquorice.

[0006] As a further technical solution, the raw materials include the following components in parts by weight: 40-45 parts of Coptis chinensis, 25-30 parts of Phellodendron chinense, 40-45 parts of Taraxacum herb, 20-30 parts of Patrinia chinensis, 30-35 parts of Astragalus membranaceus, 85-90 parts of Qinpi, 30-35 parts of Atractylodes lancea, 60-65 parts of Poria cocos, 20-25 parts of Alisma orientalis, 25-30 parts of Tangerine peel, 15-20 parts of stir-fried Shenqu, 20-25 parts of stir-fried Hawthorn, 15-20 parts of stir-fried malt, 15-20 parts of Angelica sinensis, 20-25 parts of Cyperus rotundus, 15-20 parts of Curcuma zedoaria, and 10-15 parts of roasted Licorice root.

[0007] As a further technical solution, the raw materials include the following components in parts by weight: 42 parts of Coptis chinensis, 28 parts of Phellodendron chinense, 41 parts of Taraxacum officinale, 26 parts of Patrinia scabra, 32 parts of Astragalus membranaceus, 89 parts of Qinpi, 32 parts of Atractylodes macrocephala, 62 parts of Poria cocos, 21 parts of Alisma orientalis, 26 parts of Tangerine peel, 18 parts of stir-fried Shenqu, 22 parts of stir-fried Hawthorn, 18 parts of stir-fried malt, 16 parts of Angelica sinensis, 23 parts of Cyperus rotundus, 18 parts of Curcuma zedoaria, and 12 parts of roasted Licorice root.

[0008] The present invention also provides a method for preparing a veterinary Chinese medicine oral liquid for preventing and treating poultry adenomyotic gastritis, comprising the following steps: S1, mixing coptis root and phellodendron amurense and extracting them with ethanol, filtering to obtain alcohol-extracted medicinal residues and a filtrate, and concentrating the filtrate under reduced pressure to obtain an extract; S2, soaking the remaining raw materials in water, adding the alcohol-extracted medicinal residue, using a water decoction method to obtain a decoction, and filtering after concentration to obtain a clear liquid; S3, after mixing the extract with the clear liquid, adding auxiliary materials to prepare the liquid, packaging and sterilizing to obtain a finished product of veterinary Chinese medicine oral liquid; The auxiliary materials include 0.01 g / mL sodium benzoate, 0.0003 g / mL Tween 80, and 0.0001 g / mL PEG400.

[0009] As a further technical solution, the extraction method of the clear liquid is step-by-step extraction, wherein coptis root and phellodendron amurense are first extracted with ethanol, mixed with other raw materials and then extracted with water.

[0010] The invention adopts a step-by-step extraction preparation method, firstly, the coptis chinensis and the phellodendron chinense are subjected to alcohol extraction, and then to water extraction, the alcohol extraction can better dissolve the alkaloid components, destroy the plant cell structure, and make the effective components more easily released, thereby increasing the extraction amount of the effective components in the coptis chinensis and the phellodendron chinense, promoting the weight gain of poultry, and enhancing the prevention and treatment effect of the veterinary Chinese medicine oral liquid on poultry adenomyosis.

[0011] As a further technical solution, the extract during the ethanol extraction also includes polysorbate, and the mass ratio of the ethanol to the polysorbate is 400:2-3.

[0012] As a further technical solution, the HLB value of the polysorbate is in the range of 13.3 to 15.6.

[0013] In the present invention, polysorbate with a certain range of HLB value is added to adjust the balance between the hydrophilicity and lipophilicity of the extract, improve the solubility of coptis root and phellodendron chinense in ethanol, reduce the aggregation and precipitation of effective ingredients, maintain the stable dispersion state of effective ingredients in the extract, further improve the extraction effect, promote the weight gain of poultry, and enhance the preventive and therapeutic efficacy of veterinary Chinese medicine oral liquid.

[0014] As a further technical solution, the HLB value of the polysorbate is 15.

[0015] As a further technical solution, the particle size of the Coptis chinensis and the Phellodendron chinense is 0.8-1.2 cm, the concentration of the ethanol is 65wt%, the time of the ethanol extraction is 3.5-4.5h, the temperature of the ethanol extraction is 38-42°C, and the ethanol extraction is stirred for 5min every 0.5h.

[0016] In the present invention, ethanol with a concentration of 65wt% is used as the extracting liquid, the solubility of the effective ingredients in Coptis chinensis and Phellodendron amurense is balanced, and suitable extraction temperature and time are combined, so that the effective ingredients can be fully extracted within a certain period of time without causing excessive thermal damage to the effective ingredients, thereby improving production efficiency and reducing production costs.

[0017] As a further technical solution, the pH value of the extract is 4.5, and the solution used to adjust the pH is a 0.05 g / mL phosphoric acid solution.

[0018] As a further technical solution, the water decoction method is divided into a first water decoction and a second water decoction, the time of the first water decoction is 1.5-2.5h, the time of the second water decoction is 45-75min, and the drug content of the clear liquid is 1g / mL.

[0019] The present invention also proposes the application of the veterinary Chinese medicinal oral liquid for preventing and treating poultry adenomyotic gastritis or the veterinary Chinese medicinal oral liquid for preventing and treating poultry adenomyotic gastritis prepared by the preparation method in the prevention and treatment of poultry adenomyotic gastritis.

[0020] The working principle and beneficial effects of the present invention are: In the present invention, polysorbate in different mass ratios is added as one of the extracting solutions, the surface tension of the extraction system is reduced, the diffusion of ethanol in the medicinal material is promoted, and the ethanol can penetrate into the medicinal material more quickly and fully contact with the effective ingredients, thereby improving the extraction efficiency, adjusting the viscosity of the extracting solution, facilitating subsequent filtering and separation operations, shortening the extraction time, effectively promoting the weight gain of poultry, and enhancing the preventive and therapeutic efficacy of the oral solution. DETAILED DESCRIPTION

[0021] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0022] Example 1 S1. Weigh 40 parts of Coptis chinensis and 25 parts of Phellodendron chinense, crush them into granules with a particle size of 0.8 cm using a crusher, add 520 parts of 65wt% ethanol and 1.3 parts of polysorbate (HLB value is 10, molecular weight is 648), extract at 38°C for 3.5 hours, stir for 5 minutes every 0.5 hours, filter to obtain alcohol-extracted drug residues and filtrate, concentrate the filtrate under reduced pressure, add 0.05g / mL phosphoric acid solution, adjust the pH value to about 4.5, and obtain an extract; S2, weigh 40 parts of dandelion, 20 parts of Patrinia, 30 parts of Astragalus, 85 parts of Qinpi, 30 parts of Atractylodes, 60 parts of Poria, 20 parts of Alisma, 25 parts of Tangerine Peel, 15 parts of stir-fried Shenqu, 20 parts of stir-fried Hawthorn, 15 parts of stir-fried Malt, 15 parts of Angelica, 20 parts of Cyperus, 15 parts of Curcuma, 10 parts of Radix Glycyrrhizae Preparata, put them into a multifunctional extraction tank, add 3360 parts of extraction water, soak for 3.5 hours, add Coptis chinensis and Phellodendron chinense residues, decoct for 1.5 hours, collect the distillate, and filter out decoction I; add 2520 parts of extraction water to the extraction tank again, decoct for 45 minutes, filter out decoction II, and reduce the pressure to concentrate decoction I and decoction II to contain about 1g / mL of crude drug to obtain a concentrated solution; refrigerate the concentrated solution and let it stand for 12 hours, filter out the precipitate with an oscillating sieve to obtain a clear solution; S3. After the extract is mixed with the clear liquid and the distillate, 0.01 g / mL of sodium benzoate, 0.0003 g / mL of Tween 80 and 0.0001 g / mL of PEG400 are added to prepare the liquid. After packaging and sterilization, the finished product of the veterinary Chinese medicine oral liquid is obtained.

[0023] Example 2 S1. Weigh 42 parts of Coptis chinensis and 28 parts of Phellodendron chinense, crush them into granules with a particle size of 1 cm using a crusher, add 560 parts of 65wt% ethanol and 1.4 parts of polysorbate (HLB value is 10, molecular weight is 648), extract at 40°C for 4 hours, stir for 5 minutes every 0.5 hours, filter the decoction, concentrate under reduced pressure, add 0.05g / mL phosphoric acid solution, adjust the pH value to about 4.5, and obtain an extract; S2, weigh 41 parts of dandelion, 26 parts of Patrinia, 32 parts of Astragalus, 89 parts of Qinpi, 32 parts of Atractylodes, 62 parts of Poria, 21 parts of Alisma, 26 parts of Tangerine Peel, 18 parts of stir-fried Shenqu, 22 parts of stir-fried Hawthorn, 18 parts of stir-fried Malt, 16 parts of Angelica, 23 parts of Cyperus, 18 parts of Curcuma, 12 parts of Radix Glycyrrhizae Preparata, put them into a multifunctional extraction tank, add 3648 parts of extraction water, soak for 4 hours, add Coptis chinensis and Phellodendron chinense residues, decoct for 2 hours, collect the distillate, and filter out decoction I; add 2736 parts of extraction water to the extraction tank again, decoct for 60 minutes, filter out decoction II, and reduce the pressure to concentrate decoction I and decoction II to contain about 1g / mL of crude drug to obtain a concentrated solution; refrigerate the concentrated solution and let it stand for 12 hours, filter out the precipitate with an oscillating sieve to obtain a clear solution; S3. After the extract is mixed with the clear liquid and the distillate, 0.01 g / mL of sodium benzoate, 0.0003 g / mL of Tween 80 and 0.0001 g / mL of PEG400 are added to prepare the liquid. After packaging and sterilization, the finished product of the veterinary Chinese medicine oral liquid is obtained.

[0024] Example 3 S1. Weigh 45 parts of Coptis chinensis and 30 parts of Phellodendron chinense, crush them into granules with a particle size of 1.2 cm using a crusher, add 600 parts of 65wt% ethanol and 1.5 parts of polysorbate (HLB value is 10, molecular weight is 648), extract at 42°C for 4.5 hours, stir for 5 minutes every 0.5 hours, filter the decoction, concentrate under reduced pressure, add 0.05g / mL phosphoric acid solution, adjust the pH value to about 4.5, and obtain an extract; S2, weigh 45 parts of dandelion, 30 parts of Patrinia, 35 parts of Astragalus, 90 parts of Qinpi, 35 parts of Atractylodes, 65 parts of Poria, 25 parts of Alisma, 30 parts of Tangerine peel, 20 parts of stir-fried Shenqu, 25 parts of stir-fried Hawthorn, 20 parts of stir-fried Malt, 20 parts of Angelica, 25 parts of Cyperus, 20 parts of Curcuma, 15 parts of Radix Glycyrrhizae Preparata, put them into a multifunctional extraction tank, add 4000 parts of extraction water, soak for 4.5 hours, add Coptis chinensis and Phellodendron chinense residues, decoct for 2.5 hours, collect the distillate, and filter out decoction I; add 3000 parts of extraction water to the extraction tank again, decoct for 75 minutes, filter out decoction II, and reduce the pressure to concentrate decoction I and decoction II to contain about 1g / mL of crude drug to obtain a concentrated solution; refrigerate the concentrated solution and let it stand for 12 hours, filter out the precipitate with an oscillating sieve to obtain a clear solution; S3. After the extract is mixed with the clear liquid and the distillate, 0.01 g / mL of sodium benzoate, 0.0003 g / mL of Tween 80 and 0.0001 g / mL of PEG400 are added to prepare the liquid. After packaging and sterilization, the finished product of the veterinary Chinese medicine oral liquid is obtained.

[0025] Example 4 Compared with Example 2, the difference of Example 4 is that the added amount of polysorbate (HLB value is 10, molecular weight is 648) is 2.8 parts.

[0026] Example 5 Compared with Example 2, the difference in Example 5 is that the added amount of polysorbate (HLB value is 10, molecular weight is 648) is 4.2 parts.

[0027] Example 6 Compared with Example 2, the difference in Example 6 is that the added amount of polysorbate (HLB value is 10, molecular weight is 648) is 5.6 parts.

[0028] Example 7 Compared with Example 5, the difference of Example 7 is that 4.2 parts of polysorbate (HLB value is 10, molecular weight is 648) are replaced by an equal amount of polysorbate (HLB value is 13.3, molecular weight is 522).

[0029] Example 8 Compared with Example 5, the difference of Example 8 is that 4.2 parts of polysorbate (HLB value is 10, molecular weight is 648) are replaced by an equal amount of polysorbate (HLB value is 15, molecular weight is 1309.63).

[0030] Example 9 Compared with Example 5, Example 9 is different in that 4.2 parts of polysorbate (HLB value is 10, molecular weight is 648) are replaced with an equal amount of polysorbate (HLB value is 15.6, molecular weight is 1284.6).

[0031] Example 10 Compared with Example 5, the difference of Example 10 is that 4.2 parts of polysorbate (HLB value is 10, molecular weight is 648) are replaced by an equal amount of polysorbate (HLB value is 16.7, molecular weight is 1226.48).

[0032] Embodiment 11 Compared with Example 2, the difference of Example 11 is that the preparation method of the extract comprises the following steps: Weigh 42 parts of Coptis chinensis and 28 parts of Phellodendron chinense, crush them into particles with a particle size of 1 cm using a crusher, add 560 parts of 65wt% ethanol and extract at 40°C for 4 hours, stir for 5 minutes every 0.5 hours, filter the decoction, concentrate under reduced pressure, add 0.05g / mL phosphoric acid solution, adjust the pH to about 4.5, and obtain an extract.

[0033] Comparative Example 1 Compared with Example 2, the difference of Comparative Example 1 is that the preparation method of the veterinary Chinese medicine oral liquid comprises the following steps: S1, weigh 42 parts of coptis root and 28 parts of phellodendron, crush them into granules with a particle size of 1 cm with a crusher, weigh 41 parts of dandelion, 26 parts of patrinia, 32 parts of astragalus, 89 parts of qinpi, 32 parts of atractylodes, 62 parts of tuckahoe, 21 parts of oriental rhizome, 26 parts of dried tangerine peel, 18 parts of stir-fried Shenqu, 22 parts of stir-fried hawthorn, 18 parts of stir-fried malt, 16 parts of angelica, 23 parts of cyperus, 18 parts of curcuma, 12 parts of roasted liquorice, put them into a multifunctional extraction tank, add 3648 parts of extraction water, soak for 4 hours, add the crushed coptis root and phellodendron, decoct for 2 hours, collect the distillate, and filter the decoction I; add 2736 parts of extraction water to the extraction tank again, decoct for 60 minutes, filter the decoction II, and reduce the pressure to concentrate the decoction I and decoction II to contain about 1g / mL of crude drug to obtain a concentrated solution; refrigerate the concentrated solution and let it stand for 12 hours, filter out the precipitate with an oscillating sieve to obtain a clear solution; S2. Add 0.01 g / mL of sodium benzoate, 0.0003 g / mL of Tween 80 and 0.0001 g / mL of PEG400 to the clear liquid to prepare the liquid, and then pack and sterilize to obtain the finished product of veterinary Chinese medicine oral liquid.

[0034] Comparative Example 2 Compared with Example 2, the difference of Comparative Example 2 is that the preparation method of the clear solution comprises the following steps: The alcohol-extracted residues of Coptis chinensis and Phellodendron amurense were put into 560 parts of extraction water, soaked for 4 hours, decocted for 2 hours, and the decoction was filtered; 41 parts of Taraxacum officinale, 26 parts of Patrinia scabra, 32 parts of Astragalus, 89 parts of Qinpi, 32 parts of Atractylodes macrocephala, 62 parts of Poria, 21 parts of Alisma orientalis, 26 parts of Citrus reticulata, 18 parts of stir-fried Shenqu, 22 parts of stir-fried Crataegus pinnatifida, 18 parts of stir-fried Malt, 16 parts of Angelica sinensis, 23 parts of Cyperus rotundus, 18 parts of Curcuma zedoaria, and 12 parts of roasted Licorice root were weighed and put into a multifunctional extraction tank, 3648 parts of extraction water were added, soaked for 4 hours, decocted for 2 hours, the distillate was collected, and decoction I was filtered; 2736 parts of extraction water were added to the extraction tank again, decocted for 60 minutes, and decoction II was filtered, and the decoction, decoction I and decoction II were concentrated under reduced pressure to contain about 1 g / mL of crude drug to obtain a concentrated solution; the concentrated solution was refrigerated and allowed to stand for 12 hours, and the precipitate was filtered out with an oscillating sieve to obtain a clear solution.

[0035] Test 1 The veterinary Chinese medicine oral liquids prepared in Examples 1 to 11 and Comparative Examples 1 to 2 were respectively taken by ultraviolet spectrophotometry to detect the concentration of berberine therein. The detection method refers to the Chinese Pharmacopoeia 2020 edition "Pharmacopoeia of the People's Republic of China".

[0036] Table 1 Berberine content in veterinary Chinese medicine oral solution in Examples 1 to 11 and Comparative Examples 1 to 2

[0037] Compared with Example 2, Comparative Example 1 was directly subjected to water extraction, while Comparative Example 2 Coptis chinensis and Phellodendron chinense were first subjected to alcohol extraction and then to water extraction alone. As a result, the berberine content in Example 2 was higher than that in Comparative Examples 1 and 2, indicating that when the extraction step is to first extract Coptis chinensis and Phellodendron chinense with alcohol and then to extract with other raw materials with water, the berberine content in the obtained extract is higher.

[0038] Compared with Example 11, polysorbate was added in Example 2, and the berberine content in Example 2 was higher than that in Example 11, indicating that the addition of polysorbate can further improve the dissolution of berberine; in Example 2 and Examples 4 to 6, ethanol and polysorbate were added in different mass ratios, and the berberine content in Examples 4 to 5 was higher than that in Examples 2 and 6, indicating that when the mass ratio of ethanol to polysorbate was 400:2 to 3, the berberine content in the obtained extract was higher.

[0039] Compared with Example 5, Examples 7 to 10 added polysorbates with different HLB values. As a result, the berberine content of Examples 7 to 9 was greater than that of Examples 5 and 10, indicating that when the HLB value of polysorbate was 13.3 to 15.6, and preferably 15, the berberine content in the obtained extract was higher.

[0040] Test 2 The veterinary Chinese medicine oral liquid prepared in Example 8 was applied to a flock of white-feathered broiler chickens suffering from adenomyotic gastritis.

[0041] A chicken farm in Lingshou County, Shijiazhuang, has a total of 2,000 white-feathered broilers aged 30 days, which are raised in two sheds, with 1,000 chickens in each shed. An average of 2 to 3 chickens die every day. The two sheds are randomly divided into a control group and a test group. The control group has a normal diet, and the test group is fed with the veterinary Chinese medicine oral liquid prepared in Example 8 twice a day, 50 mL of the Chinese medicine oral liquid is diluted with 180 L of water each time, and used for 5 consecutive days. The feed, drinking water and utensils used during the test are strictly separated to avoid cross infection. The health status, feeding and feces of the chicks are observed three times a day, and the incidence and mortality of white-feathered broilers in each group are recorded; Symptoms of disease: lethargy, ruffled feathers, slow growth, and loss of appetite; Incidence rate = total number of diseased chickens in each group / total number of test chickens in each group × 100%; Mortality rate = total number of dead chickens in each group / total number of experimental chickens in each group × 100%.

[0042] The test results are shown in Table 2 below: Table 2 Clinical observation record of 5-day use of veterinary Chinese medicine oral solution

[0043] As shown in Table 2, the morbidity and mortality rates of the control group that did not take the oral liquid were higher than those of the experimental group, the feed intake was less than that of the experimental group, and the feather condition and mental state of the broilers were poor. In the experimental group that took the oral liquid, the morbidity and mortality rates of white-feathered broilers were greatly reduced, the feed intake of the broilers increased, the feathers became smoother and neater, the digestive state of the broilers was good, the spirits were full, and the eyes were bright, indicating that the veterinary Chinese medicine oral liquid can effectively prevent and treat adenomyotic gastritis in white-feathered broilers.

[0044] Test 3 280 20-day-old broilers with adenomyotic gastritis of similar weight were selected as experimental subjects and randomly divided into 14 groups, each with 20 chickens, namely 1 control group and 13 experimental groups. The 13 experimental groups were fed with the veterinary Chinese medicine oral liquid prepared in Examples 1 to 11 and Comparative Examples 1 to 2, twice a day. Each experimental group was fed with 0.5 mL of the Chinese medicine oral liquid diluted with 1.8 L of water each time for 10 consecutive days. Each chicken was weighed before and after the experiment on an empty stomach for 12 hours. The average daily increase of each group of experimental broilers was recorded to judge their growth status and the preventive and therapeutic effect of adenomyotic gastritis.

[0045] The test results are shown in Table 3 below: Table 3 Test results of Chinese medicinal oral liquids for preventing and treating poultry adenomyotic gastritis prepared in the control group, Examples 1 to 11 and Comparative Examples 1 to 2

[0046] Compared with the control group, the broilers in the experimental group took the veterinary Chinese medicine oral liquid for preventing and treating poultry adenomyosis gastritis every day. The results showed that the average daily weight gain of the experimental group was greater than that of the control group, indicating that the veterinary Chinese medicine oral liquid for preventing and treating poultry adenomyosis gastritis has a better preventive and therapeutic effect.

[0047] Compared with Comparative Examples 1 and 2, in Example 2, Coptis chinensis and Phellodendron chinense are first subjected to alcohol extraction, and then water extraction is performed with other raw materials. Comparative Example 1 is directly subjected to water extraction, and in Comparative Example 2, Coptis chinensis and Phellodendron chinense are first subjected to alcohol extraction, and then water extraction is performed separately. As a result, the average daily weight gain of Example 2 is greater than that of Comparative Examples 1 and 2, indicating that the step-by-step extraction process of first alcohol extraction and then mixed water extraction can increase the content of effective ingredients in the veterinary Chinese medicine oral liquid, increase the daily weight gain of poultry, and contribute to the preventive and therapeutic effect of the veterinary Chinese medicine oral liquid for preventing and treating poultry adenomyotic gastritis.

[0048] Compared with Example 11, Example 2 added polysorbate, and the average daily weight gain of Example 2 was greater than that of Example 11, indicating that the addition of polysorbate can increase the content of active ingredients in the veterinary Chinese medicine oral liquid, increase the average daily weight gain of poultry, and improve the preventive and therapeutic effect of the veterinary Chinese medicine oral liquid for preventing and treating poultry adenomyotic gastritis.

[0049] In Example 2 and Examples 4 to 6, ethanol and polysorbate were added in different mass ratios. As a result, the average daily weight gain of Examples 4 to 5 was greater than that of Examples 2 and 6, indicating that when the mass ratio of ethanol to polysorbate was 400:2 to 3, the obtained veterinary Chinese medicine oral liquid for preventing and treating poultry adenomyotic gastritis had better prevention and treatment effect.

[0050] Compared with Example 5, Examples 7 to 10 added polysorbates with different HLB values. As a result, the average daily weight gain of Examples 7 to 9 was greater than that of Examples 5 and 10, indicating that when the HLB value of polysorbate was 13.3 to 15.6, and preferably 15, the content of active ingredients in the obtained veterinary Chinese medicine oral liquid for preventing and treating poultry adenomyotic gastritis was higher, the daily weight gain of poultry could be increased, and the prevention and treatment effect was better.

[0051] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A veterinary Chinese medicine oral liquid for preventing and treating poultry adenomyotic gastritis, characterized in that: The raw materials include the following components in parts by weight: 30-50 parts of coptis root, 20-30 parts of phellodendron, 40-50 parts of dandelion, 20-30 parts of patrinia herb, 30-40 parts of astragalus, 80-100 parts of qinpi, 20-40 parts of atractylodes, 50-70 parts of tuckahoe, 10-30 parts of oriental water plantain, 20-40 parts of tangerine peel, 10-30 parts of stir-fried Shenqu, 20-30 parts of stir-fried hawthorn, 10-30 parts of stir-fried malt, 10-30 parts of angelica, 20-30 parts of cyperus rotundus, 10-30 parts of curcuma, and 10-20 parts of roasted liquorice.

2. The veterinary Chinese medicine oral liquid for preventing and treating poultry adenomyotic gastritis according to claim 1, characterized in that: The raw materials include the following components in parts by weight: 40-45 parts of coptis root, 25-30 parts of phellodendron, 40-45 parts of dandelion, 20-30 parts of patrinia herb, 30-35 parts of astragalus, 85-90 parts of qinpi, 30-35 parts of atractylodes, 60-65 parts of tuckahoe, 20-25 parts of oriental water plantain, 25-30 parts of tangerine peel, 15-20 parts of stir-fried Shenqu, 20-25 parts of stir-fried hawthorn, 15-20 parts of stir-fried malt, 15-20 parts of angelica, 20-25 parts of cyperus rotundus, 15-20 parts of curcuma, and 10-15 parts of roasted liquorice.

3. A method for preparing a veterinary Chinese medicine oral liquid for preventing and treating poultry adenomyotic gastritis according to claim 1 or 2, characterized in that: The following steps are involved: S1, mixing coptis root and phellodendron amurense and extracting them with ethanol, filtering to obtain alcohol-extracted medicinal residues and a filtrate, and concentrating the filtrate under reduced pressure to obtain an extract; S2, soaking the remaining raw materials in water, adding the alcohol-extracted medicinal residue, using a water decoction method to obtain a decoction, and filtering after concentration to obtain a clear liquid; S3, after mixing the extract with the clear liquid, adding auxiliary materials to prepare the liquid, packaging and sterilizing to obtain a finished product of veterinary Chinese medicine oral liquid; The auxiliary materials include 0.01 g / mL sodium benzoate, 0.0003 g / mL Tween 80, and 0.0001 g / mL PEG400.

4. The method for preparing a veterinary Chinese medicine oral liquid for preventing and treating poultry adenomyotic gastritis according to claim 3, characterized in that: The extraction method of the clear liquid is step-by-step extraction. Coptis chinensis and Phellodendron chinense are firstly extracted with ethanol to obtain an ethanol extract; and the drug residues are mixed with other raw materials and then extracted with water.

5. The method for preparing a veterinary Chinese medicine oral liquid for preventing and treating poultry adenomyotic gastritis according to claim 3, characterized in that: During the ethanol extraction, the extract also includes polysorbate, and the mass ratio of the ethanol to the polysorbate is 400:2-3.

6. The method for preparing a veterinary Chinese medicine oral liquid for preventing and treating poultry adenomyotic gastritis according to claim 5, characterized in that: The HLB value of the polysorbate is in the range of 13.3 to 15.

6.

7. The method for preparing a veterinary Chinese medicine oral liquid for preventing and treating poultry adenomyotic gastritis according to claim 3, characterized in that: The particle sizes of the coptis chinensis and the phellodendron amurense are 0.8-1.2 cm, the concentration of the ethanol is 65 wt %, the time of the ethanol extraction is 3.5-4.5 h, the temperature of the ethanol extraction is 38-42° C., and the extraction is stirred for 5 min every 0.5 h.

8. The method for preparing a veterinary Chinese medicine oral liquid for preventing and treating poultry adenomyotic gastritis according to claim 3, characterized in that: The pH value of the extract is 4.5, and the solution used to adjust the pH is a 0.05 g / mL phosphoric acid solution.

9. The method for preparing a veterinary Chinese medicine oral liquid for preventing and treating poultry adenomyotic gastritis according to claim 3, characterized in that: The water decoction method is divided into a first water decoction and a second water decoction. The time of the first water decoction is 1.5 to 2.5 hours, the time of the second water decoction is 45 to 75 minutes, and the drug content of the clear solution is 1 g / mL.

10. Use of the veterinary Chinese medicinal oral liquid for preventing and treating poultry adenomyotic gastritis according to any one of claims 1 to 2 or the veterinary Chinese medicinal oral liquid for preventing and treating poultry adenomyotic gastritis prepared by the preparation method according to any one of claims 3 to 9 in the prevention and treatment of poultry adenomyotic gastritis.

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